For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
TEPAPDPARPLAGVRVVEISSFVAVPLAGMTLAQLGAEVLRVDPVGGAADYRRWPLTESGDSIYWAGLNK GKKSLAVDMRSGQGQALVARLIADAGVFITNVAGRQWHSYDALRTDRPDLIHAEVSGRHDGGTGVDYTVN AAIGFPLVTGPPELSTPVNHVLPAWDVTCGIYTALSVVTALRHRDATGHGQQISIPLENVALATAGNLSL LTEAMINGTSRQRIGNAVYGTYGQNFTSSDGVSFMVVALTGRHFRDLNALTGTTESVAALSRSLDVDFSD EGERYRHREALGGLYAEWFSAHTGAEISAALSETSVLWERYQTFAETAADARVTDNPLFTELDQPRVGTH LAAGLPVSIDGVYPRAVPAPALGDDTDSVLGDWLSLDAARIDELRETGTVA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4144 | - | - | 100% (402) | L-carnitine dehydratase/bile acid-inducible protein F |
| M. gilvum PYR-GCK | Mflv_4465 | - | 8e-30 | 26.42% (405) | Formyl-CoA transferase |
| M. gilvum PYR-GCK | Mflv_4365 | - | 5e-29 | 28.12% (409) | L-carnitine dehydratase/bile acid-inducible protein F |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3300 | - | 3e-20 | 28.31% (378) | hypothetical protein Mb3300 |
| M. tuberculosis H37Rv | Rv3272 | - | 4e-20 | 28.31% (378) | hypothetical protein Rv3272 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4651c | - | 2e-27 | 26.98% (404) | putative L-carnitine dehydratase |
| M. marinum M | MMAR_4031 | - | 1e-140 | 60.96% (397) | acyl-CoA transferase/carnitine dehydratase |
| M. avium 104 | MAV_1574 | - | 1e-139 | 61.21% (397) | L-carnitine dehydratase/bile acid-inducible protein F |
| M. smegmatis MC2 155 | MSMEG_2468 | - | 1e-176 | 74.07% (405) | L-carnitine dehydratase/bile acid-inducible protein F |
| M. thermoresistible (build 8) | TH_2496 | - | 6e-33 | 27.14% (409) | PUTATIVE L-carnitine dehydratase/bile acid-inducible protein F |
| M. ulcerans Agy99 | MUL_3896 | - | 1e-137 | 59.95% (397) | acyl-CoA transferase/carnitine dehydratase |
| M. vanbaalenii PYR-1 | Mvan_2197 | - | 0.0 | 88.06% (402) | L-carnitine dehydratase/bile acid-inducible protein F |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_4144|M.gilvum_PYR-GCK MTEP---APDPARPLAGVRVVEISSFVAVPLAGMTLAQLGAEVLRVDPVG
Mvan_2197|M.vanbaalenii_PYR-1 MTEP---APDPARPLAGVRVVEISSFVAVPLAGMTLAQLGAEVLRVDPVG
MSMEG_2468|M.smegmatis_MC2_155 MAEPVAHTPDPTRPLAGVRIIEISSFVAVPLAGMTLAQLGAEVVRVDPIG
MMAR_4031|M.marinum_M -------MPGPDLPLSGISVVEVSSFVAAPLCGMTLSQLGAQVIRVDPIG
MUL_3896|M.ulcerans_Agy99 -------MPGPDLPLSGISVVEVSSFVAAPLCGMTLSQLGAQVIRVDPIG
MAV_1574|M.avium_104 -------MTDPRLPLHGLSVVEVSSFVAAPLCGMTLSQLGAEVIRVDPIG
Mb3300|M.bovis_AF2122/97 -----MPTSNPAKPLDGFRVLDFTQNVAGPLAGQVLVDLGAEVIKVEAPG
Rv3272|M.tuberculosis_H37Rv -----MPTSNPAKPLDGFRVLDFTQNVAGPLAGQVLVDLGAEVIKVEAPG
TH_2496|M.thermoresistible__bu ---MAESEHADPAPLAGITVVALEQAVSAPMCTRVLADFGARVIKVENPD
MAB_4651c|M.abscessus_ATCC_199 ----------MTGALDGIRVLELGTLIAGPFAGRLLGDMGAEVLKIEPPG
.* *. :: . :: *:. * ::**.*:::: .
Mflv_4144|M.gilvum_PYR-GCK GAADYRRWPLTESGD--SIYWAGLNKGKKSLAVDMRSGQGQALVARLIAD
Mvan_2197|M.vanbaalenii_PYR-1 GAADYKRWPLTEAGD--SIYWAGLNKGKRSLAVDMRSDTGQELVARLIAD
MSMEG_2468|M.smegmatis_MC2_155 GAADYHRWPVTDGGD--SIYWAGLNKGKRSVAADMRSPEGQQVVQRLIAE
MMAR_4031|M.marinum_M GASDIRRWPLAASGT--SIYWTGLNKGKRSATIDLRSAAGQRLVQRLVLE
MUL_3896|M.ulcerans_Agy99 GASDIRRWPLAASGT--SIYWTGLNKGKRSATIDLRSAAGQRLVQRLVLE
MAV_1574|M.avium_104 GASDVQRWPLAADGT--SIYWTGLNKGKRSATIDLRSAEGQRLVQRLIVE
Mb3300|M.bovis_AF2122/97 GEAARQITSVLPGRPPLATYFLPNNRGKKSVTVDLTTEQAKQQMLRLADT
Rv3272|M.tuberculosis_H37Rv GEAARQITSVLPGRPPLATYFLPNNRGKKSVTVDLTTEQAKQQMLRLADT
TH_2496|M.thermoresistible__bu GGDFARHYDDVVKGQ--AAHFVWANRGKESVTLNLKSEAGMAVLHRLLDE
MAB_4651c|M.abscessus_ATCC_199 APDPLRTWGQAEVDG-HHVFWTVHARNKKAITLDLRTEAGRALFLDLVDK
. : .: :.*.: : :: : . . *
Mflv_4144|M.gilvum_PYR-GCK A-GVFITNVAG---RQWHSYDALRTDRPDLIHAEVSGRHDGG-----TGV
Mvan_2197|M.vanbaalenii_PYR-1 S-GVFITNVAG---RQWHSYDALRQIRPDLIHVEVSGRHDGG-----TGV
MSMEG_2468|M.smegmatis_MC2_155 S-GVLITNAAG---RQWHSYEELAALRSDLIHVEVSGRADGG-----TGV
MMAR_4031|M.marinum_M GDGVVVTNAS----LPWLSHEVLAATRSDIIHLQLLGRRDGG-----TGV
MUL_3896|M.ulcerans_Agy99 GDGVVVTNAS----LPWLSHEVLAATRSDVIHLQLLGRRDGG-----TGV
MAV_1574|M.avium_104 GDGVVVTNAAG---LSWLSHDALAALRADVIHVQLLGRGDGS-----TGV
Mb3300|M.bovis_AF2122/97 ADVVLEAFRPGTMEKLGLGPDDLRSRNPNLIYARLTAYGGNGPHGSRPGI
Rv3272|M.tuberculosis_H37Rv ADVVLEAFRPGTMEKLGLGPDDLRSRNPNLIYARLTAYGGNGPHGSRPGI
TH_2496|M.thermoresistible__bu ADVLVSNLAPGALARMGLAADDLKTSHPRVLPVQIDGYGAGGPISHKRAY
MAB_4651c|M.abscessus_ATCC_199 SDVIVENFRPGTLERWGLGYDVLAQRNKGIILVRVSGYGQTGPDAHKAGY
. :. . . : * . :: .: . . .
Mflv_4144|M.gilvum_PYR-GCK DYTVNAAIGFPLVTGPPELSTPVNHVLPAWDVTCGIYTALSVVTALRHRD
Mvan_2197|M.vanbaalenii_PYR-1 DYTVNAAIGFPLVTGPPELTTPVNHVLPAWDVTCGIYAALSVVTALRHRD
MSMEG_2468|M.smegmatis_MC2_155 DYTVNAALGFPLVTGPAEVSTPVNHVLPAWDVSCGLYVALAVTAALRHRD
MMAR_4031|M.marinum_M DYTVNAATGFPLVTGPADHAGPINHVLPAWDVSCGLYAALSVVAALRRRD
MUL_3896|M.ulcerans_Agy99 DYTVNAATGFPLVTGPADHAGPINHVLPAWDVSCGLYAALVVVAALRRRD
MAV_1574|M.avium_104 DYTVNAGIGYPLVTGPAEHAGPINHVLPAWDVCCGLYAALSVVTAVHRRE
Mb3300|M.bovis_AF2122/97 DLVVAAEAGMTTGMPTPEGK-PQIIPFQLVDNASGHVLAQAVLAALLHRE
Rv3272|M.tuberculosis_H37Rv DLVVAAEAGMTTGMPTPEGK-PQIIPFQLVDNASGHVLAQAVLAALLHRE
TH_2496|M.thermoresistible__bu DLLIQAESGACAVTG--YPGMPAKPGPPVADISTGLYAALSVMALLYGRD
MAB_4651c|M.abscessus_ATCC_199 ASVAEAASGLRHLNGFPGGP-PPRMALSIGDTLAGMFAAQGALAALYRRT
* * * * * * . : : *
Mflv_4144|M.gilvum_PYR-GCK AT-----GHGQQISIPLENVALATAGNLSLLTEAMING---------TSR
Mvan_2197|M.vanbaalenii_PYR-1 AT-----GHGQQISIPLENVALATAGNLSLLTEAMING---------TSR
MSMEG_2468|M.smegmatis_MC2_155 AT-----GEGARISVPLENVALATAGNLGFLTEVMING---------TSR
MMAR_4031|M.marinum_M QT-----GAGAQISLALEDVALATAGNLGLLTEPQVNG---------TQR
MUL_3896|M.ulcerans_Agy99 QT-----GAGAQISLALEDVALATAGNLGLLTEPQVNG---------TQR
MAV_1574|M.avium_104 QS-----GAGARISLALEDVALATAANLGLLTEPQVVG---------TQR
Mb3300|M.bovis_AF2122/97 RN-----GVADVVQVAMYDVAVGLQANQLMMHLNRAASDQPKPEPAPKAK
Rv3272|M.tuberculosis_H37Rv RN-----GVADVVQVAMYDVAVGLQANQLMMHLNRAASDQPKPEPAPKAK
TH_2496|M.thermoresistible__bu RRDRATPGPVPAVAVSLFDTMTDIMGYQLTYTQHSGVD---------QQP
MAB_4651c|M.abscessus_ATCC_199 VT-----GRGQVVDAALTESCLAVQESTIPDYDVGRVVR----------G
* : .: :
Mflv_4144|M.gilvum_PYR-GCK QRIGNAVYGTYGQNFTSSDGVSFMVVALTGRHFRDLNALTGTTESVAALS
Mvan_2197|M.vanbaalenii_PYR-1 QRIGNAVYGTYGQNFTSSDGISYMVVALTGRHFRDLTALTGTTEAVAVLA
MSMEG_2468|M.smegmatis_MC2_155 ERLGNSLFGTYGQNFTSSDGVFFMLVALTGRHFRDLTEVTGTTKAVAALG
MMAR_4031|M.marinum_M ERLGNSIYGQYGQDFASRDGTSFMVITLTRRHFRDLVDVTGTGPAVTALA
MUL_3896|M.ulcerans_Agy99 ERLGNSIYGQYGQDFASRDGTSFMVITLTRRHFRDLVDVTGTGPAVTALA
MAV_1574|M.avium_104 ERLGNAIYGQYGQDFTSRDGARFMVVTLTGRHFRDLLDVTGTGAAVSALA
Mb3300|M.bovis_AF2122/97 RRKGVGFATQPSDAFRTADG-YIVISAYVPKHWQKLCYLIGRPDLVE---
Rv3272|M.tuberculosis_H37Rv RRKGVGFATQPSDAFRTADG-YIVISAYVPKHWQKLCYLIGRPDLVE---
TH_2496|M.thermoresistible__bu LGMSSPAVAPYG-AFRTRDGQTVVLGTTNDREWQRLARDIIERPDLAD--
MAB_4651c|M.abscessus_ATCC_199 PSGTRLEGIAPSNIYQSADGSWVVIAANQDTVFRRLCEAMGRPELSS---
. : : ** :: : :: *
Mflv_4144|M.gilvum_PYR-GCK RSLDVDFSDEGERYRHREALGGLYAEWFSAHTGAEISAALSETSVLWERY
Mvan_2197|M.vanbaalenii_PYR-1 DSLGADFSDEGERYRHREALSGLFAEWFTSHTGVEVAAALSATSVLWERY
MSMEG_2468|M.smegmatis_MC2_155 EALGADFTDEGQRYTHRDALTGLFTLWFSEHTADEITEALSGTSVLWERY
MMAR_4031|M.marinum_M EALGVDFDAEGDRYRYRSVLNGLFAAWFADHTAEQIGAALSATTVLFERY
MUL_3896|M.ulcerans_Agy99 EALGVDFDAEGDRYRYRSVLNGLFAAWFADHRAEQIGAALSATTVLFERY
MAV_1574|M.avium_104 DALGVDFGSEGERYRYRDVLSGLFATWFGDHTADEITAALSHTTVLFERY
Mb3300|M.bovis_AF2122/97 ---DQRFAEQRSRSINYAELTAELELALASKTATEWVQLLQANGLMACLA
Rv3272|M.tuberculosis_H37Rv ---DQRFAEQRSRSINYAELTAELELALASKTATEWVQLLQANGLMACLA
TH_2496|M.thermoresistible__bu ---DPAYATNSDRVRHRDVLNEAIAAWCARHDLAHIQKTADDAGIGNSRY
MAB_4651c|M.abscessus_ATCC_199 ---DARFANHVARGRNQDDLDAIIAQWAAQRLPTDIIDVLSAAGVIAGPI
. : . * * : . . :
Mflv_4144|M.gilvum_PYR-GCK QTFAETAADARVTDNPLFTELDQPRVGTHLAAG--LPVSIDGVYPRAVPA
Mvan_2197|M.vanbaalenii_PYR-1 QTFGETAAAPRVTDNPLFTELDQPRVGRYLAAG--LPLSINGAYPAAVPA
MSMEG_2468|M.smegmatis_MC2_155 RTFAQTAGDRRVTDNPLFTPLDQPRIGRYLAPG--LPVSIGGVYPAAVAA
MMAR_4031|M.marinum_M RTFAEVAADPKVTANPLFSQLSQPGVGDYLAPG--LPTAFDGCHPAGAPA
MUL_3896|M.ulcerans_Agy99 RTFAEVAADPKVTANPLFSQLSQPGVGDYFAPG--LPTAFDGCHPAGAPA
MAV_1574|M.avium_104 RTFAEVAAGPKVTANPLFSRLHQPGLGDYFAPG--LPAAFDGTHPAGAPA
Mb3300|M.bovis_AF2122/97 HTWKQVVDTPLFAESDLTLEVGR-GADTITVIR--TPARYASFRAVVTDP
Rv3272|M.tuberculosis_H37Rv HTWKQVVDTPLFAENDLTLEVGR-GADTITVIR--TPARYASFRAVVTDP
TH_2496|M.thermoresistible__bu NLPSEVVAHPHLSARDRWRPVDT-PNGQITALR--PPPVIEGFEQPMGPV
MAB_4651c|M.abscessus_ATCC_199 NTVADVVQDPQLKAREMLVEHFDERLGRSVLGPGVVPVLSESPGGVRNAG
. :.. . . * .
Mflv_4144|M.gilvum_PYR-GCK PA-LGDDTDSVLGDWLSLDAARIDELRETGTVA-----------
Mvan_2197|M.vanbaalenii_PYR-1 PA-LGDDTAAVLGDWLAMDRGRIDDLFGAGTVA-----------
MSMEG_2468|M.smegmatis_MC2_155 PA-LGDDTAAVLAEHLGLSAEKIAELTESGTVATGSAQK-----
MMAR_4031|M.marinum_M PA-LGEHTADVLTEYLGLTTADIARLASAKTIGGPDGQQDKEYA
MUL_3896|M.ulcerans_Agy99 PA-LGEHTADVLTEYLGLTTADTARLASAKTIGGPDGQQDKEYA
MAV_1574|M.avium_104 PA-LGQDTAEVLAR-LGLTAADIERLADAKTIAC----------
Mb3300|M.bovis_AF2122/97 PP-TAGEHNAVFLARP----------------------------
Rv3272|M.tuberculosis_H37Rv PP-TAGEHNAVFLARP----------------------------
TH_2496|M.thermoresistible__bu PG-LGQHTDAVLSGLLGMTDDEIAALREQGAIGPAYN-------
MAB_4651c|M.abscessus_ATCC_199 PARPGQDNDDVYVGLLGKSAQDIERLRAEGVL------------
* . . *