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ARFPKPAEGSWTQHYPELGTGPVSYEDSISPEIYELERQAIFKRAWLNVGRVELLPRKGSYFTKELKAAN TSIIVVRNKSGKINAFHNVCRHRGNKLVWDDLPQQETSGVCRQFTCKYHAWRYDLDGDLTFVQQEEEFFD LDKSRYGLVPVHCQVWEGFIFVNFAQEPEQSLRDFLGPMITNLEGYPFDKLTSRFTYRSEVKANWKLYMD AFQEFYHAPVLHANQSPTAYSRAAAEAGFEAPHYRIEGPHRLVSTSGVRAWEMPDEMRKPIEDICQSGLF GPWDKPDLGEMPVGLNPAKCDPWGLDSFQLFPNFVMLFWGQGWYLTYHYWPTSYNTHTFECTLYFPQART PRERLAQELAAASFKEYGLQDANTLEATQSMIETRVVEEFLLCDQEILLRHLHSETAAWIEDYQRKTAGV *
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_0299 | - | - | 100% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_4840 | - | e-151 | 57.51% (426) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_0293 | - | e-133 | 51.80% (417) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 7e-22 | 29.47% (207) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_2464 | - | 0.0 | 85.51% (421) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 2e-21 | 28.99% (207) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 7e-20 | 32.16% (199) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 1e-154 | 59.48% (422) | dioxygenase |
| M. avium 104 | MAV_3967 | - | 0.0 | 86.26% (422) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_3362 | - | 0.0 | 85.24% (420) | PUTATIVE Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_4884 | - | 3e-15 | 29.76% (205) | dioxygenase |
| M. vanbaalenii PYR-1 | Mvan_4190 | - | 0.0 | 85.51% (421) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 ------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTS---SELW
Rv3161c|M.tuberculosis_H37Rv ------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTS---SELW
Mflv_2464|M.gilvum_PYR-GCK ---------------MARFPKPPEGSWTEHYPQLGTGPVSYEDSIDPEFY
Mvan_4190|M.vanbaalenii_PYR-1 ---------------MARFPKPPEGSWTQHYPQLGTGPVSYRDSVDPAFY
TH_3362|M.thermoresistible__bu ---------------MARFPKPPEGSWTEHHPELGTAPVSYRDCTDPEIY
MSMEG_0299|M.smegmatis_MC2_155 ---------------MARFPKPAEGSWTQHYPELGTGPVSYEDSISPEIY
MAV_3967|M.avium_104 ---------------MARFPKPPEGSWTQHYPELGTAPVSYEDSISPEVY
MMAR_4744|M.marinum_M ---------------MPHFTKPAAGSWTQNYPELGTAPVDYTDSIDPQYF
MUL_4884|M.ulcerans_Agy99 MSSPTKPLVAEDAEIVRRVLAHIDAGTTDEGEAWREPVENYAS---PERF
MAB_4370c|M.abscessus_ATCC_199 -MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELY
: . :
Mb3186c|M.bovis_AF2122/97 QLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPV----MVVRDVDGQ
Rv3161c|M.tuberculosis_H37Rv QLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPV----MVVRDVDGQ
Mflv_2464|M.gilvum_PYR-GCK ELERKAVFRRAWLNVGRVEQVPRKGSYFTKEMKVVNT--SIIVVRTTSGE
Mvan_4190|M.vanbaalenii_PYR-1 ELERKAVFRRAWLNVGRVEQIPRKGSYFTKEMKVVNT--SIIVVRTTTGE
TH_3362|M.thermoresistible__bu QKERHAIFKRAWLNVGRVERLPRKGSYFTKEIKVVNT--SIIVVRTGSGE
MSMEG_0299|M.smegmatis_MC2_155 ELERQAIFKRAWLNVGRVELLPRKGSYFTKELKAANT--SIIVVRNKSGK
MAV_3967|M.avium_104 ELEREAIFKRAWLNVARVEQLPRKGSYLTRELKVVNT--SIILVRNGSGE
MMAR_4744|M.marinum_M ADEQRAIFRRTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGV
MUL_4884|M.ulcerans_Agy99 AAELRLLRGMPSVFVP-SAAIPNNGDHVERTAFGVPL----FAVRGADGR
MAB_4370c|M.abscessus_ATCC_199 RQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPV----IVSMDKNRR
* . : . : : . . *... :
Mb3186c|M.bovis_AF2122/97 LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Rv3161c|M.tuberculosis_H37Rv LHALSPICRHR------LMLMVEPGAGRIDTLTCQYHLWRYGLDGRLRGA
Mflv_2464|M.gilvum_PYR-GCK VKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
Mvan_4190|M.vanbaalenii_PYR-1 VKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
TH_3362|M.thermoresistible__bu IKAFHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFV
MSMEG_0299|M.smegmatis_MC2_155 INAFHNVCRHRGNKLVWDDLPQQETSGVCRQFTCKYHAWRYDLDGDLTFV
MAV_3967|M.avium_104 IKAFHNVCRHRGNKLVWNDMPLEETRGVCRQFTCKYHAWRYDLDGNLTFV
MMAR_4744|M.marinum_M IRAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFI
MUL_4884|M.ulcerans_Agy99 ARVFRNARRHRG-------FALVENAGCSHALVCRYHGWIYRLDGSLSHV
MAB_4370c|M.abscessus_ATCC_199 IRAFLNSCRHRG------ARVCRADYGVTRGFTCTYHGWAYDLEGKLISV
.. *** * :.* ** * * *:* *
Mb3186c|M.bovis_AF2122/97 PHMAANLD-FNRRECRLPQFAVATWNGLVWINLDADAEP-IAAHLDLTDD
Rv3161c|M.tuberculosis_H37Rv PHMAANLD-FNRRECRLPQFAVATWNGLVWINLDADAEP-IAAHLDLTDD
Mflv_2464|M.gilvum_PYR-GCK QQEGEFFD-LDKSRYGLVPVHCEVWEGFIFVNFAPEPEQSLREFLGPMIT
Mvan_4190|M.vanbaalenii_PYR-1 QQEGEFFD-LDKSRYGLVPVHCDVWEGFIFVNFAKQPEQSLRDFLGPMIT
TH_3362|M.thermoresistible__bu QQEEEFFD-LHKSRYGLVPVHCDVWEGFIFVNFAEQPEQSLREFLGPMIT
MSMEG_0299|M.smegmatis_MC2_155 QQEEEFFD-LDKSRYGLVPVHCQVWEGFIFVNFAQEPEQSLRDFLGPMIT
MAV_3967|M.avium_104 QQEEEFFD-LDKSRYGLVPVHCDVWEGFVFVNFAKTPEQSLREFLGPMIT
MMAR_4744|M.marinum_M QQEDEFFD-VDKSQYGLKSVRCEVWEGFIFINLDP-AAAPLSEYLGSMAK
MUL_4884|M.ulcerans_Agy99 PHAEAFPD-LDMSARGLVEVESHEVDGLIVIGPLDPVDAGLIEDEGAIGW
MAB_4370c|M.abscessus_ATCC_199 PNEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDMAW
: * . * . : .. : .
Mb3186c|M.bovis_AF2122/97 EFAGYRLGEMVQ-----VESWSHEWRANWKVAAENGH-ENYHVLGLHRQT
Rv3161c|M.tuberculosis_H37Rv EFAGYRLGEMVQ-----VESWSHEWRANWKVAAENGH-ENYHVLGLHRQT
Mflv_2464|M.gilvum_PYR-GCK NLEGYPFDRMTS-----RFHYRSEVKANWKLYMDAFQ-EFYHAPVLHANQ
Mvan_4190|M.vanbaalenii_PYR-1 DLEGYPFDRMTS-----RFHYRSEVKANWKLYMDAFQ-EFYHAPVLHANQ
TH_3362|M.thermoresistible__bu GLEGYPFDRMTS-----RFTYRSEVKANWKLYMDAFQ-EFYHAPVLHANQ
MSMEG_0299|M.smegmatis_MC2_155 NLEGYPFDKLTS-----RFTYRSEVKANWKLYMDAFQ-EFYHAPVLHANQ
MAV_3967|M.avium_104 ALEGYPFDKMTS-----RWCYRSEVKANWKLYMDAFQ-EFYHAPVLHANQ
MMAR_4744|M.marinum_M GLQGYPFHEMTE-----VYSYRAEVGSNWKLFIDAFA-EFYHAPVLHQGQ
MUL_4884|M.ulcerans_Agy99 LTDGSPWRAKLVPAERLIVVESTLREINWKVLVEQFL-EGYHIRSTHKDT
MAB_4370c|M.abscessus_ATCC_199 YMDAMLDHDSEG-TVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHASA
. ***: : : ** *
Mb3186c|M.bovis_AF2122/97 ---------------LEPFVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS
Rv3161c|M.tuberculosis_H37Rv ---------------LEPFVPGGGDLDVRQYSRWALRLRVPFTVPVEAKS
Mflv_2464|M.gilvum_PYR-GCK SPTAYSKAAAQAGFEAPHYQIDGPHRLVSTSGVRAWEMADEMRKPIEDIC
Mvan_4190|M.vanbaalenii_PYR-1 SPTAYSKAAAEAGFEAPHYRIEGPHRLVSTSGIRAWELADEMRKPIEDIC
TH_3362|M.thermoresistible__bu SPTAYSKAAAEAGFEAPHYRIDGPHRLVSTSGVRAWEMSEEMRKPIEDIC
MSMEG_0299|M.smegmatis_MC2_155 SPTAYSRAAAEAGFEAPHYRIEGPHRLVSTSGVRAWEMPDEMRKPIEDIC
MAV_3967|M.avium_104 SPTAYSKAAAEAGFEAPHYRLDGPHRLVSTSGVRAWEMSPEMRKPIEDIC
MMAR_4744|M.marinum_M YTKEEAAKIMKYGFEALHYELAGPHAMISVWGGQSPPADLKMVKPMDRVL
MUL_4884|M.ulcerans_Agy99 -----------------FFPVQYDDLNVVEAFGPNTRITFPYRN-IERLR
MAB_4370c|M.abscessus_ATCC_199 LMVMAPQGKAP---KDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVH
.
Mb3186c|M.bovis_AF2122/97 -----------------------LQLNEVQKSNLVVLWTFPNSALAIAG-
Rv3161c|M.tuberculosis_H37Rv -----------------------LQLNEVQKSNLVVLWTFPNSALAIAG-
Mflv_2464|M.gilvum_PYR-GCK QSGLFGPWDKP----DLGEMPAGLNPAKCDPWGLDSFQLFPNFVILFWGQ
Mvan_4190|M.vanbaalenii_PYR-1 ESGLFGPWDKP----DLGEMPVGLNPAKCDPWGLDSFQLFPNFVMLFWGQ
TH_3362|M.thermoresistible__bu RSGLFGPWDKP----DLGEMPKGLNPARCDPWGLDSFQLFPNFVILFWGQ
MSMEG_0299|M.smegmatis_MC2_155 QSGLFGPWDKP----DLGEMPVGLNPAKCDPWGLDSFQLFPNFVMLFWGQ
MAV_3967|M.avium_104 RSGLFGPWDKP----DLGPMPDGLNPAKCDPWGLDSFQLFPNFVILFWGQ
MMAR_4744|M.marinum_M RSGLFGPWDRPAPITDLEELPPGINPTKAKQWGIDSFLFFPNFMLLIWEP
MUL_4884|M.ulcerans_Agy99 DRP--------------------EHTWTTGARVTYLYQLFPNVMLATFP-
MAB_4370c|M.abscessus_ATCC_199 QWTDYGALRER-----------LGEARVQGPLTNGHATVFPNLSYLPVNG
. ***
Mb3186c|M.bovis_AF2122/97 ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN---
Rv3161c|M.tuberculosis_H37Rv ERVVWFGFIPQSIDRVQVLGGVLTTPELAADAAATAQTSQFVMAMIN---
Mflv_2464|M.gilvum_PYR-GCK GWYLTYHYWPTSHNTHIFEGTVYFPQPRTPRERIAQELAAVSFKEYG---
Mvan_4190|M.vanbaalenii_PYR-1 GWYLTYHYWPTSHNTHLFEGTVYFPQPRTPRERIAQELAAVSFKEYG---
TH_3362|M.thermoresistible__bu GWYLTYHYWPTSYNTHVFEGNLYFPAARTPRERVAQELAAASFKEYG---
MSMEG_0299|M.smegmatis_MC2_155 GWYLTYHYWPTSYNTHTFECTLYFPQARTPRERLAQELAAASFKEYG---
MAV_3967|M.avium_104 GWYLTYHYWPTSYHSHVFEGTLYFPAPRTPRERVAQELAAVSFKEYG---
MMAR_4744|M.marinum_M GWYLTYHYWPTTVDKHIFETTLYFVPPRNARERLAQELAAVTFKEYA---
MUL_4884|M.ulcerans_Agy99 DVIAMVVIDPVSVDRTAVTIYSMVTPDVAERDSLDASSNLAGAGSFIGQG
MAB_4370c|M.abscessus_ATCC_199 SVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIFEQD
* . . .
Mb3186c|M.bovis_AF2122/97 -DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPG
Rv3161c|M.tuberculosis_H37Rv -DEDRLGLEAVQVGAGSRFAERGHLSSKEWPGMLAFYRNLAMALVGDHPG
Mflv_2464|M.gilvum_PYR-GCK -LQDANTLEATQSMVESRVLDDFVLCDQ--EVLIRHLHTETAAWVDDYRR
Mvan_4190|M.vanbaalenii_PYR-1 -LQDANTLEATQSMVESEVLDEFVLCDQ--EVLIRHLHTETAAWVDEYRR
TH_3362|M.thermoresistible__bu -LQDANTLEATQSMIESGVLDDFVLCDQ--EVLIRHLHKETADWIDRYER
MSMEG_0299|M.smegmatis_MC2_155 -LQDANTLEATQSMIETRVVEEFLLCDQ--EILLRHLHSETAAWIEDYQR
MAV_3967|M.avium_104 -LQDANTLEATQTMLESRVVDKFVLNDQ--EILLRHLHKETAAWIDDYRR
MMAR_4744|M.marinum_M -LQDSNTLEATQTMIGTGVVKDFPLCDQ--EILLRHLHKMTGDYVKEYRN
MUL_4884|M.ulcerans_Agy99 LFEDNEMSEGVQRGLAAGANAFVEFGRH--ESAVGRFHAALGRRLARLTS
MAB_4370c|M.abscessus_ATCC_199 DGENWSEVQSISRGFVTNSVALNYQMGLDSEREDGRHPGLTSELYSDAAG
:: :. . :
Mb3186c|M.bovis_AF2122/97 AS-------------------
Rv3161c|M.tuberculosis_H37Rv AS-------------------
Mflv_2464|M.gilvum_PYR-GCK K-TQQVKEGV-----------
Mvan_4190|M.vanbaalenii_PYR-1 K-TAGV---------------
TH_3362|M.thermoresistible__bu E-STGTAAGVAGR--------
MSMEG_0299|M.smegmatis_MC2_155 K-TAGV---------------
MAV_3967|M.avium_104 MSTAGV---------------
MMAR_4744|M.marinum_M NGHAK----------------
MUL_4884|M.ulcerans_Agy99 AG-------------------
MAB_4370c|M.abscessus_ATCC_199 RSFYARWRDLMNTKAWHEEHV