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TMYLTQGLHRAVQQTPGATMTVFGERRRTFLEVADRVARLAGALRALGVRAGDRVAMLAQNSDRYHEYLL AVPWANGVLNPVNIRWTPAEIAYSLEDSGTKVMFVDDSFARMLPAIKDAWAELSTVIYAGEASPPAGTLA YEELIDKSDPVDDARRGGDELAGLFYTGGTTGFPKGVMLSHANLLTSTYGLLTPGFAFRPGGICLHAAPM FHLADLMSWSAGLLQGGRHVFIPSFEPVTVMQAIAQHQVTDALLVPTMIQKLIDHPDINRYDLTSFRALF YAASAIPQALLERAMKVFPNAEFVQGYGMTELSPLATLLSPDDHQKPRLLRSAGRAAPSAEVRIVDPDGN EVPRRTVGEVAVRGGNVMLGYWNKPQETAAAIRDGWMHTGDAAYMDDDGYIYVVDRIKDMIITGGENVYS AEVENAVVQHPSVAACAVIGVPDADWGERVHAVVVLQPGAELAACQIREHAKRLIAGYKCPRSIEFVNAL PLSGAGKVLKRELRERHLGARERDRGSLP*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4065 | - | - | 100% (520) | feruloyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_0131 | - | 8e-88 | 39.03% (515) | acyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_2883 | - | 9e-79 | 35.93% (501) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0172 | fadD5 | 5e-83 | 37.15% (506) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_0027 | - | 1e-96 | 40.95% (503) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0166 | fadD5 | 1e-82 | 37.15% (506) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 2e-56 | 31.81% (503) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 1e-179 | 60.47% (511) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_0409 | fadD5 | 4e-87 | 37.18% (503) | fatty-acid-CoA ligase FadD5 |
| M. avium 104 | MAV_3523 | - | 2e-87 | 38.33% (514) | acyl-CoA synthase |
| M. thermoresistible (build 8) | TH_2878 | - | 3e-87 | 40.08% (509) | PUTATIVE AMP-binding enzyme, putative |
| M. ulcerans Agy99 | MUL_0650 | fadD8 | 3e-69 | 33.72% (513) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_3993 | - | 1e-178 | 59.39% (522) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_4065|M.smegmatis_MC2_155 -------------------------------------------MTMYLTQ
MAB_4559|M.abscessus_ATCC_1997 ---------------------------------------------MQFTQ
Mvan_3993|M.vanbaalenii_PYR-1 -----------------------------------------MSDGMYLTQ
Mb0172|M.bovis_AF2122/97 --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
Rv0166|M.tuberculosis_H37Rv --------------------MTAQLASHLTRALTLAQQQPYLARRQNWVN
MMAR_0409|M.marinum_M --------------------MTAELASHLPQAA-HALEQPYLARRQNWVN
TH_2878|M.thermoresistible__bu ------------------------------------LQQPYLARRQNWVN
Mflv_0027|M.gilvum_PYR-GCK -------------------------------------------MFHNLAD
MAV_3523|M.avium_104 --------------------------------------MPAAETRETLAG
MUL_0650|M.ulcerans_Agy99 ------------------------------------MSDDLLRNPTHNGH
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
MSMEG_4065|M.smegmatis_MC2_155 GLHRAVQQTPGATMTVFGERRRTFLEVADRVARLAGALRALGVRAGDRVA
MAB_4559|M.abscessus_ATCC_1997 WLHRALQQYPDRLITIDQGREHTVEQFADRVARLSAGLRANGVRPGDRVG
Mvan_3993|M.vanbaalenii_PYR-1 GLHRSLRATPDATMTICGERVRTFADVGDRVSRLAGGFRSLGASPGDRIA
Mb0172|M.bovis_AF2122/97 QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
Rv0166|M.tuberculosis_H37Rv QLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVM
MMAR_0409|M.marinum_M QLERHAMMQPRATAIRYLGHTVTWADLRYRVAALAGSLSRRGVGFGDRVM
TH_2878|M.thermoresistible__bu HLARHALMQPDATALRFMGQTTTWADLHHRVNRLAGALRRRGVEFGDRVL
Mflv_0027|M.gilvum_PYR-GCK IVRSHGRNRPDAPALIVGDRTVSYRELDERSNRTAQAFSRTGVGPGDRVA
MAV_3523|M.avium_104 IVERHAQRRPDAIAIRYGERQWSWAEWSSRIRRAAGALRGDGIERGQCVA
MUL_0650|M.ulcerans_Agy99 LLVGALERHKNRPVLFLGDTTLTGGQLAERISQYIQAFEALGAGTGVTVG
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
: : .: * * :
MSMEG_4065|M.smegmatis_MC2_155 MLAQNSDRYHEYLLAVPWANGVLNPVNIRWTPAEIAYSLEDSGTKVMFVD
MAB_4559|M.abscessus_ATCC_1997 MLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEIGYSLRESGTRVLLVD
Mvan_3993|M.vanbaalenii_PYR-1 ILSLNSDRYQESLLATVWSGCVTNPVNIRWSAAEIAYALRDSDSRILLID
Mb0172|M.bovis_AF2122/97 ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCAAHVMLTE
Rv0166|M.tuberculosis_H37Rv ILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVMLTE
MMAR_0409|M.marinum_M ILMLNRTEFVESVLAANMLGAIAVPLNFRLTPSEIAFLVEDCAPRLVITE
TH_2878|M.thermoresistible__bu ILMLNRTEFIETMLAANRLGAIAVPVNFRMTPPEIAFLAKDCEAKVIATE
Mflv_0027|M.gilvum_PYR-GCK FVERNGIEFFDVAFGSAKLGAVAVPVNWRLTAPEMRHVIADCGASVVVVG
MAV_3523|M.avium_104 FLDKNHPACLEVLIGGASVGAVTTVVNWRVIGDELVHVLADSGARVLVVG
MUL_0650|M.ulcerans_Agy99 LLSLNRPEVLMILGASQARGYRRTALHPLGSLDDHAYVLYDAGASALIID
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
:: * . . :: : . :
MSMEG_4065|M.smegmatis_MC2_155 --DSFARMLPAIKDAWAELSTVIYAG--EASPPAGTLAYEELIDKSD-PV
MAB_4559|M.abscessus_ATCC_1997 --DTFKAAAAPLRAACACLETVIYCG--AGPAPQDMIGYEDLLASHE-PI
Mvan_3993|M.vanbaalenii_PYR-1 --DAFLPMLPELRREFPGLGDVIHCG--DGVAPPECLAFEDLLGVSD-PV
Mb0172|M.bovis_AF2122/97 --AALAPVAIGVRNIQPLLSVIVVAG---GSSQDSVFGYEDLLNEAG-DV
Rv0166|M.tuberculosis_H37Rv --AALAPVAIGVRNIQPLLSVIVVAG---GSSQDSVFGYEDLLNEAG-DV
MMAR_0409|M.marinum_M --EVLAQVAVGVREIAPALSTIVVAG---GASDPTVVGYDELISETG-DP
TH_2878|M.thermoresistible__bu --PVLAGVATAVRDLAPSLSSVIVAGAADGSDTPDVLDYEALIAEPG-ED
Mflv_0027|M.gilvum_PYR-GCK --QEFTGRIEAIADDLDVEVVVIGKHR-------HWPTFDDLLAAAP-AV
MAV_3523|M.avium_104 --AELRPAAEAAARRVPSLERIIEVG----------DEYESLLAAAE-PA
MUL_0650|M.ulcerans_Agy99 PNPMFVERALGLLEKVDSLEQVLTIGPVPEALEDRAVDLSAEAAKYQPKP
MLBr_02546|M.leprae_Br4923 --DEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSS---
:
MSMEG_4065|M.smegmatis_MC2_155 D-DARRGGDELAGLFYTGGTTGFPKGVMLSHANLLTSTYGLLTPGFAFRP
MAB_4559|M.abscessus_ATCC_1997 D-DTRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAMGSLSTGNFLTR
Mvan_3993|M.vanbaalenii_PYR-1 T-DAHRSGSDLAGVFYTGGTSGHPKGVMLTHDNLMTSAMGALASGFFLTS
Mb0172|M.bovis_AF2122/97 HEPVDIPNDSPALIMYTAGTTGRPKGAVLTHANLTGQAMTALYTSGANIN
Rv0166|M.tuberculosis_H37Rv HEPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALYTSGANIN
MMAR_0409|M.marinum_M PEPVDIPNDSPALIMYTSGTTGRPKGAVLTHTNLTGQVMTALYTGGANIN
TH_2878|M.thermoresistible__bu PPPVDIPDDSPALIMYTSGTTGNPKGAVLTHSNLTGQAMTNLFTIGVDLA
Mflv_0027|M.gilvum_PYR-GCK DPGVVTGPDDLVFLMYTSGTTGAPKGVMLSNANVVCKTAGVGGPWKFDAD
MAV_3523|M.avium_104 PSDAGVDTDETALVIYSSGTTGRPKGVLLSQRALVNHAANLAPAFPFGDG
MUL_0650|M.ulcerans_Agy99 LVVADLPPNHIGGLTYTGGTTGKPKGVIGTAGNITAMTSIQLAEWEWPEH
MLBr_02546|M.leprae_Br4923 SAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFRAH
. . : :.**:* ***. .
MSMEG_4065|M.smegmatis_MC2_155 GGICLHAAPMFHLADLM-SWSAGLLQGGRHVFIPS--FEPVTVMQAIAQH
MAB_4559|M.abscessus_ATCC_1997 GGRLLHSAPMFHMADFS-AVLAGNLSGSTHVIVPS--FSPQGVLDAIVAH
Mvan_3993|M.vanbaalenii_PYR-1 GGPLLHAAPMFHLADLA-IWLEQVIRGGTHVFIPS--FKPEGVLDALQRH
Mb0172|M.bovis_AF2122/97 SDVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAE
Rv0166|M.tuberculosis_H37Rv SDVGFVGVPLFHIAGIG-NMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAE
MMAR_0409|M.marinum_M SDVGFIGVPFFHIAGIG-NMLSGMLLGVPTVIYPLGAFNPGQLLDVLEAE
TH_2878|M.thermoresistible__bu NDVGFIGVPLFHIAGIAGNVLSGLTLGLPTVLYPLGAFDPGGLLDVLEAE
Mflv_0027|M.gilvum_PYR-GCK -SVSLAVMPLFHMAGFG-WALAGLWEGAVTVVLRD--VDPGAILDAVARH
MAV_3523|M.avium_104 -DANLVAMPLFHVGGIG-YALFGIRAGAPTIMTRE--PDAAALIGAVRAG
MUL_0650|M.ulcerans_Agy99 -PRFLMCTPLSHAGAAF--FTPTLVKGGEMIVLAK--FDPGEVLRVIEEQ
MLBr_02546|M.leprae_Br4923 -EVTLLPAPMFHALGYL-HATLAMFLGSTLVLRRR--FKPATVLEDIEKH
: *: * * :. .. :: :
MSMEG_4065|M.smegmatis_MC2_155 QVTDALLVPTMIQKLIDHPD--INRYDLTSFRALFYAASAIPQALLERAM
MAB_4559|M.abscessus_ATCC_1997 DVQDMLLVPTMIQMLVDHPG--AAQLDLSGIRSITYGASVISEAVLQRAT
Mvan_3993|M.vanbaalenii_PYR-1 RVSDLLLVPTMVQMLVDHPA--LPVYDTSALQMVMYGGSPIAAALLERAS
Mb0172|M.bovis_AF2122/97 KVTGIFLVPAQWQAVCTEQQ--ARPRDLR-LRVLSWGAAPAPDALLRQMS
Rv0166|M.tuberculosis_H37Rv KVTGIFLVPAQWQAVCTEQQ--ARPRDLR-LRVLSWGAAPAPDALLRQMS
MMAR_0409|M.marinum_M RVSGIFLVPAQWQAVCAEQQ--ARPRELS-LRVMSWGAAPAPDALLRQMS
TH_2878|M.thermoresistible__bu KVTGIFLVPAQWQAVCAEQR--ARPRDLR-LRALSWGAAPASDTLLQEMT
Mflv_0027|M.gilvum_PYR-GCK RVTNMLLVPAVIQFLLDTTG--LDDTDLSRLRIVVYGASPITDDVLMRGI
MAV_3523|M.avium_104 -ATHAFFVPPVIARFLDAGE--AARASIAGLRYIVYGAAPMPLPLLHRAL
MUL_0650|M.ulcerans_Agy99 RITATMLVPSMLYALMDHPD--PHTRDLSSLETVYYGASAINPVRLAEAI
MLBr_02546|M.leprae_Br4923 QATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRAL
..** . . :. : ..: .
MSMEG_4065|M.smegmatis_MC2_155 KVFPNAEFVQGYGMTELSPLATLLSPDDHQ---KPRLLRSAGRAAPSAEV
MAB_4559|M.abscessus_ATCC_1997 RVFPNARFTQAYGMTEVSPVATLLLPDDHD---DPALLRSAGRAAPHCEV
Mvan_3993|M.vanbaalenii_PYR-1 KRLPTASFVQAYGMTEVSPIATLLSPRDHD---DPVLRRSGGVAAPHSEV
Mb0172|M.bovis_AF2122/97 ATFPETQILAAFGQTEMSPVTCMLLGEDA-----IAKRGSVGRVIPTVAA
Rv0166|M.tuberculosis_H37Rv ATFPETQILAAFGQTEMSPVTCMLLGEDA-----IAKRGSVGRVIPTVAA
MMAR_0409|M.marinum_M EVFPGTQIMAAFGQTEMSPVTCMLLGEDA-----IRKRGSVGKVIPTVSA
TH_2878|M.thermoresistible__bu EVFPGTKIFAAFGQTEMSPVTCMLLAEDA-----VRKLGSVGKVIPTVAA
Mflv_0027|M.gilvum_PYR-GCK DRFGG-IFAQVYGMTESTGSVTQLDGDEH----LPQLLRSCGRPYPWVQI
MAV_3523|M.avium_104 STWPGTKFVQVYGQTELCGAVTALSDDDHRDAARPQLQLSAGKAVQGCEI
MUL_0650|M.ulcerans_Agy99 DRFGP-IFAQYYGQSEAPMVITYLPKADHD----EKRLTSCGRPTLFARV
MLBr_02546|M.leprae_Br4923 EELGP-VIYNMYGSTEIAFATIAGPKDLQIN------SATVGPVVKGVKV
: :* :* : *
MSMEG_4065|M.smegmatis_MC2_155 RIVDP-DGNEVPRRTVGEVAVRGGNVMLGYWNKPQETAAAIRD-GWMHTG
MAB_4559|M.abscessus_ATCC_1997 RIVDP-DDNEVPRGEIGEVIVKGDNVMLGYWELPEESAAAIRD-GWMHTG
Mvan_3993|M.vanbaalenii_PYR-1 RIVDE-DDVEVPRGDVGEITVRGGHVMAGYWGRPEDTATALRG-GWMHTG
Mb0172|M.bovis_AF2122/97 RVVDQ-NMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFAG-GWFHSG
Rv0166|M.tuberculosis_H37Rv RVVDQ-NMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFAG-GWFHSG
MMAR_0409|M.marinum_M RVVDD-EMNDVPIGQVGEIVYRAPTLMSGYWNNPDATAEAFAG-GWFHSG
TH_2878|M.thermoresistible__bu RIVDD-TMNDVPVGEVGEIVYRAPTLMAGYWNNPQATAEAFAG-GWFHSG
Mflv_0027|M.gilvum_PYR-GCK RVVDP-TGRDVAPGTVGEVWTRSDQNMLGYWNNPEATAATLTPDGWLRTG
MAV_3523|M.avium_104 RIVDPNNCAELPAGRSGEVWVRSNQNMSGYLNRAEATAETITADGWVRTG
MUL_0650|M.ulcerans_Agy99 ALLGE-DGKPVPQGEPGEICVSGPLLAGGYWNKPDDTAQTFRD-GWLHTG
MLBr_02546|M.leprae_Br4923 KILDD-SGKEVPRGQVGRIFVGNAFPFEGYTG---GGGKQIID-GLLSSG
::. :. *.: * . : * . :*
MSMEG_4065|M.smegmatis_MC2_155 DAAYMDDDGYIYVVDRIKDMIITGGENVYSAEVENAVVQHPSVAACAVIG
MAB_4559|M.abscessus_ATCC_1997 DAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVENALAKHPAVAACAVIG
Mvan_3993|M.vanbaalenii_PYR-1 DAGYMDERGYVFVVDRIKDMIVSGGENVYSVEVENALARHPAVAACAVIG
Mb0172|M.bovis_AF2122/97 DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
Rv0166|M.tuberculosis_H37Rv DLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIG
MMAR_0409|M.marinum_M DLVRMDEDGYVWVVDRKKDMIISGGENVYCAEVENVLASHPSIVEVAVIG
TH_2878|M.thermoresistible__bu DLVRQDEEGYIWVVDRKKDMIISGGENIYCAEVENVLAAHPAVAEVAVVG
Mflv_0027|M.gilvum_PYR-GCK DAGYLDRDGYLYLHDRIKDMIVSGAENVYPAEVENVLMTHPAVTDVAVIG
MAV_3523|M.avium_104 DVGRLDADGYVYIEDRLKDMIITGGENVYGPEVESVLIEHPAVVDAAVIG
MUL_0650|M.ulcerans_Agy99 DMAREDEDGFYFIVDRVKDMIVTGGFNVFPREVEDVVAEHSAVAQVCVVG
MLBr_02546|M.leprae_Br4923 DVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIG
* * . :: .* .:*:::*. *:: *:*. : *. :. .:*
MSMEG_4065|M.smegmatis_MC2_155 VPDADWGERVHAVVVLQPGAELAAC-------QIREHAKRLIAGYKCPRS
MAB_4559|M.abscessus_ATCC_1997 IPDEQWGERVHAVVVKQAQSECCDG-------DLQEHCREHIANYKVPRS
Mvan_3993|M.vanbaalenii_PYR-1 VPDERWGERVHAVVVPSAEGGADARNLD--PEELREFCRSLIGGYKVPRT
Mb0172|M.bovis_AF2122/97 RADEKWGEVPIAVAAVT-NDDLRIE-------DLGEFLTDRLARYKHPKA
Rv0166|M.tuberculosis_H37Rv RADEKWGEVPIAVAAVT-NDDLRIE-------DLGEFLTDRLARYKHPKA
MMAR_0409|M.marinum_M RADEKWGEVPIAVAAVT-KDHLRIE-------DLDEYLTERLARYKHPKA
TH_2878|M.thermoresistible__bu RPHEKWGEVPVAVVALNPDAELTMA-------ELEEFLADRLARYKHPKV
Mflv_0027|M.gilvum_PYR-GCK IPDTRWGEAVKAVVVAG--APVTEA-------ELIAFAREQLAGFKLPKS
MAV_3523|M.avium_104 VPDDFWGESVKAIVVAD--GDVDAA-------DVIEFCRRHLAGFKCPRT
MUL_0650|M.ulcerans_Agy99 APDEKWGEAVTAVVVLRADAARDDAAIETMTAEIQAAVKQRKGSVQSPKQ
MLBr_02546|M.leprae_Br4923 VDDKEWGARLRAFVVKKQDATIDED-------AIKAYVRDHLARYKVPRE
. ** *... : . : *:
MSMEG_4065|M.smegmatis_MC2_155 IEFVNALPLSGAGKVLKRELRERHLGARERDRGSLP-------------
MAB_4559|M.abscessus_ATCC_1997 FEFVDELPLSGAGKILKRVLRQKYWESRDAGVS----------------
Mvan_3993|M.vanbaalenii_PYR-1 VEVVAALPLSAAGKVLKRELRARYWEGSDRSVG----------------
Mb0172|M.bovis_AF2122/97 LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
Rv0166|M.tuberculosis_H37Rv LEIVDALPRNPAGKVLKTELRLRYGACVNVERRSASAGFTERRENRQKL
MMAR_0409|M.marinum_M LEIVDALPRNPSGKVLKTELRLRYGVGVYSESRSVSRSFEFREGS----
TH_2878|M.thermoresistible__bu LEIVDALPRNPAGKVLKTELRARFAAGPSAEKEQSSSGATTSAGSS---
Mflv_0027|M.gilvum_PYR-GCK VDFVDALPRNPSGKLLKRQLREPYWT--DEDRHIG--------------
MAV_3523|M.avium_104 VDFVAELPRNASGKILKTQLREPFWR--DRDRRV---------------
MUL_0650|M.ulcerans_Agy99 VVVVDSLPLTGLGKPDKKAVRARFWEGAGRAVG----------------
MLBr_02546|M.leprae_Br4923 VIFLEELPRNPTGKVLKRELRDFEVH-----------------------
. .: ** . ** * :*