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MSLLHQLISSVAAAAPHRPAIIADDGSTIGYAEFDHQIRAVARWIASRTEPGDRVAVIADNSPAYAVLYY AVPLGGRVLTLINQRLSPAEQAAQLETTRPALVLGDTVYLDALPQIDSVAFGSPAWRDAVSTTAAAPEVD HAEAQPGDPAWLLFTSGSTGVPKGVVHSHRSILAAVHGTVDGRSVTPAGVYLLPFPMCHIAGYNMLVQHA VGATVVLMSRFRPESFAAQVRAHGVRSCSLAPTMLHALLAHVESTGTTLPTLEAVAYGSAAMPLDLLRRA IEALGVEFHQGYGMTETGGNVTFLGPDDHRAGAAGHPEVLVSAGHPHSGVEIGIVDADGAPLPPGGIGEI VVRGAQVARGYWPDRPSTVDGWLHTGDIGRLDHTGRLFVVDRLKDIIVTGGENVSSREVEDVLSGHPDVD MVAVVGVPDGYWGEAVCAVVVPVPGRNPRADDLIDHVRSAIAAFKRPREVLFVDELPLTGNGKIAKDRVR DIARTALTA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_3694 | - | - | 100% (499) | feruloyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_4065 | - | 2e-75 | 37.98% (466) | feruloyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_4883 | - | 9e-68 | 35.43% (494) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0276 | fadD2 | 2e-55 | 31.55% (504) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_2385 | - | 4e-69 | 36.20% (489) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv0270 | fadD2 | 2e-55 | 31.55% (504) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 1e-53 | 30.66% (512) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4559 | - | 2e-73 | 37.14% (490) | putative long-chain-fatty-acid CoA ligase |
| M. marinum M | MMAR_0466 | - | 6e-64 | 34.62% (491) | long-chain-fatty-acid-CoA ligase |
| M. avium 104 | MAV_1843 | - | 1e-68 | 35.22% (494) | AMP-dependent synthetase and ligase |
| M. thermoresistible (build 8) | TH_3281 | - | 1e-176 | 63.64% (506) | PUTATIVE feruloyl-CoA synthetase |
| M. ulcerans Agy99 | MUL_1115 | - | 3e-62 | 34.28% (493) | long-chain-fatty-acid-CoA ligase |
| M. vanbaalenii PYR-1 | Mvan_4263 | - | 6e-71 | 36.31% (493) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_3694|M.smegmatis_MC2_155 --------------------------------------------------
TH_3281|M.thermoresistible__bu --------------------------------------------------
MAB_4559|M.abscessus_ATCC_1997 --------------------------------------------------
MMAR_0466|M.marinum_M --------------------------------------------------
MUL_1115|M.ulcerans_Agy99 --------------------------------------------------
MAV_1843|M.avium_104 --------------------------------------------------
Mflv_2385|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4263|M.vanbaalenii_PYR-1 --------------------------------------------------
Mb0276|M.bovis_AF2122/97 MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
Rv0270|M.tuberculosis_H37Rv MPNLTDLPGQAVSKLQKSIGQYVARGTAELHYLRKIIESGAIGLEPPLNY
MLBr_02546|M.leprae_Br4923 -----------MTKLR----HYLERGAAEVHYLRSIFESGALGFEPPLTY
MSMEG_3694|M.smegmatis_MC2_155 ---------MSLLHQLISSVAAAAPHRPAIIADDGSTIGYAEFDHQIRAV
TH_3281|M.thermoresistible__bu ---------MTLIHDLIEHAAHATPDRPAVATPGGTVITFAEFDRLVRNV
MAB_4559|M.abscessus_ATCC_1997 ----------MQFTQWLHRALQQYPDRLITIDQGREHTVEQFADRVARLS
MMAR_0466|M.marinum_M ----------MSVSLLLEMAASASPDRIAVVSGDLRLTTQQLSDLADGGA
MUL_1115|M.ulcerans_Agy99 ----------MSVSLLLEMAASASPDRIAVVSGDLRLKTQQLSDLADGGA
MAV_1843|M.avium_104 -----MKAEALSISLLLEMAVSEDPDRVALVDGANRLTLGELSSLADGGA
Mflv_2385|M.gilvum_PYR-GCK ----------MSISLLLEMATDAGPDRTAVVSDDTRLTTAELSALADGGA
Mvan_4263|M.vanbaalenii_PYR-1 ----------MSISLLLEMAQDAGPDRTAVVSDDLRLTTAELSALADGGA
Mb0276|M.bovis_AF2122/97 AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
Rv0270|M.tuberculosis_H37Rv AALAADIRKWGEVGMLPSHNARRAPNRAAVIDEEGTLTFSELDEAAHAVA
MLBr_02546|M.leprae_Br4923 AAMAVAMRKWGDLAMLPAMPARRSPHRAALIDEEGTLTYKELDRAAHALA
. *.*
MSMEG_3694|M.smegmatis_MC2_155 ARWIASRTEPGDRVAVIADNSPAYAVLYYAVPLGGRVLTLINQRLSPAEQ
TH_3281|M.thermoresistible__bu ASWLAAHSRRGDRIAVVADNSPAYAVLYYAVPRSGRVLALINQRLHPDEQ
MAB_4559|M.abscessus_ATCC_1997 AGLRANGVRPGDRVGMLSLNSDRYIEYLTAVPWLGAALNAVNIRWSLAEI
MMAR_0466|M.marinum_M GAIAASD---AQHVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
MUL_1115|M.ulcerans_Agy99 GAIAASD---AQHVVYVGTGGAMLPLLIFASARAGLPFTSINYRLSADGI
MAV_1843|M.avium_104 GVIAASG---AQHVAYVGSGVTMAPLLIFASARAGNAYTPINYRLSAGGI
Mflv_2385|M.gilvum_PYR-GCK GVIAASG---ARHVAYVGTGGALLPLLLFASARAAVPVTPLNYRLSAEGL
Mvan_4263|M.vanbaalenii_PYR-1 GVIAASG---ARHVAYVGAGGAMLPLLLFASARAAVPVTPLNYRLSADGL
Mb0276|M.bovis_AF2122/97 NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
Rv0270|M.tuberculosis_H37Rv NGLLAKGVRAGDGVAILARNHRWFVIANYGAARVGARIILLNSEFSGPQI
MLBr_02546|M.leprae_Br4923 NGLIAKGVRGGDGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQI
* . : :. . . . . :* .
MSMEG_3694|M.smegmatis_MC2_155 AAQLETTRPALVLGDTVYLDALP-------QIDSVA-FGSPAWRDAVS--
TH_3281|M.thermoresistible__bu VAQLVRTGATVLFGDPEHLAALSNSAALHGQLDHVVGFGGAGWRQVLA--
MAB_4559|M.abscessus_ATCC_1997 GYSLRESGTRVLLVDDTFKAAAAPLRAACACLETVIYCGAGPAPQDMIGY
MMAR_0466|M.marinum_M AALIRRLPRPLVVVDERYRDTI--AADQSLSSQQFLELAGRAEPVAEFP-
MUL_1115|M.ulcerans_Agy99 AALIRRLPRPLVVVDERYRETI--AADQSLSSQQFLELAGRAEPVAEFP-
MAV_1843|M.avium_104 QELIVRLPDPLVIVDSRYRDMVGDVGKLVMDSDEFLAAARAADPVAEFA-
Mflv_2385|M.gilvum_PYR-GCK RALLDRLPDPLVVVDDEYRDAVG-DSYRTMGSREFFEASRTAEPAAEFA-
Mvan_4263|M.vanbaalenii_PYR-1 RALLDRLPDPLVVVDDEYRDAVG-DGYRTIGSAEFLDAARTAEPAAEFA-
Mb0276|M.bovis_AF2122/97 KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
Rv0270|M.tuberculosis_H37Rv KEVSDREGAKVIIYDDEYTKAVSLAQPPLGKLRALGVNPDDDKPSGSSDE
MLBr_02546|M.leprae_Br4923 KEVSEREGAKVIIYDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDE
::. * . .
MSMEG_3694|M.smegmatis_MC2_155 ---TTAAAPEVDHAEAQPGDPAWLLFTSGSTGVPKGVVHSHRSILAAVHG
TH_3281|M.thermoresistible__bu ---TTSGP--VD--PPGPDDPAWLLFTSGSTGTPKPVLHTHRSVLAGVWG
MAB_4559|M.abscessus_ATCC_1997 -EDLLASHEPIDDTRTGGDSLLGVFYTGGTTGNPKGVMLSHNNVLASAMG
MMAR_0466|M.marinum_M ----------------DPDSVAIVLFTSGTTSQPKAVELTHSNLTSYVTT
MUL_1115|M.ulcerans_Agy99 ----------------DPDSVAIVLFTSGTTSQPKAVELTHSNLTSYVTT
MAV_1843|M.avium_104 ----------------DPEDVAIVLFTSGTTSKPKAVELTHGNLTSYITG
Mflv_2385|M.gilvum_PYR-GCK ----------------DPDSVGVVLFTSGTTSKPKAVELTHTNLTSYIMG
Mvan_4263|M.vanbaalenii_PYR-1 ----------------DPDAVGVVLFTSGTTSKPKAVELSHANLTSYIMG
Mb0276|M.bovis_AF2122/97 TLAELIAHSSTAPAPKASRRASIIILTSGTTGTPKGANRNTPPTLAPIGG
Rv0270|M.tuberculosis_H37Rv TLAELIAHSSTAPAPKASRRASIIILTSGTTGTPKGANRNTPPTLAPIGG
MLBr_02546|M.leprae_Br4923 TLAELIARSSSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGG
:: *.*:*. ** . . :
MSMEG_3694|M.smegmatis_MC2_155 TVD-GRSVTPAGVYLLPFPMCHIAGYN-MLVQHAVGATVVLMSRFRPESF
TH_3281|M.thermoresistible__bu TVA-GRSVQPSAVYLLPFPMCHIAGYN-MLVHHAVGATVVLVDRFRPETS
MAB_4559|M.abscessus_ATCC_1997 SLSTGNFLTRGGRLLHSAPMFHMADFSAVLAGNLSGSTHVIVPSFSPQGV
MMAR_0466|M.marinum_M TVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLTNFDAHEW
MUL_1115|M.ulcerans_Agy99 TVEFGCAAP-SDAALICVPPYHIAGVSAVLSNLYAGRKMVYLNNFDAHEW
MAV_1843|M.avium_104 TVEFGSAEP-EDAALICVPPYHIAGVNAAISNLYAGRKMVYLANFDAREW
Mflv_2385|M.gilvum_PYR-GCK TVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLRHFDAEKW
Mvan_4263|M.vanbaalenii_PYR-1 TVEFASAEP-DDAALICVPPYHIAGVSAALSNLYAGRKMVYLTQFDAGRW
Mb0276|M.bovis_AF2122/97 ILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPALV
Rv0270|M.tuberculosis_H37Rv ILSHVPFKA-GEVTLLPSPMFHALGYMHAALAMFLGSTLVLRRRFKPALV
MLBr_02546|M.leprae_Br4923 ILSKVPFRA-HEVTLLPAPMFHALGYLHATLAMFLGSTLVLRRRFKPATV
: * * * . * . * * .
MSMEG_3694|M.smegmatis_MC2_155 AAQVRAHGVRSCSLAPTMLHALLAHVESTG--TTLPTLEAVAYGSAAMPL
TH_3281|M.thermoresistible__bu AQAVIEYGVTSCSLAPTMLHSLLAHLTATG--ARLPTLSAVAYGSAAMPA
MAB_4559|M.abscessus_ATCC_1997 LDAIVAHDVQDMLLVPTMIQMLVDHPGAAQ--LDLSGIRSITYGASVISE
MMAR_0466|M.marinum_M VRLTNAEQVTTATVVPTMLDRIVAVLETGE--HKLPSLRNLAYGGSKVGL
MUL_1115|M.ulcerans_Agy99 VRLTNAEQVTTATVVPTMLDRIVAVLETGE--HKLPSLRNLAYGCSKVGL
MAV_1843|M.avium_104 VRLVAAERVTTATVVPTMLDRIVAALERDP--VELPSLRNLAYGGSKVGL
Mflv_2385|M.gilvum_PYR-GCK VRLVADEGVTSATVVPTMLDRIVTVLETTD--TALPTLRTLAYGGSKVPL
Mvan_4263|M.vanbaalenii_PYR-1 VDLVTGEGVTSATVVPTMLDRIVSVLETRG--TTLPTLRTLAYGGSKVPL
Mb0276|M.bovis_AF2122/97 LEDIEKHKATSMVVVPVMLSRILDQLEKTEPKPDLSSLKIVFVSGSQLGA
Rv0270|M.tuberculosis_H37Rv LEDIEKHKATSMVVVPVMLSRILDQLEKTEPKPDLSSLKIVFVSGSQLGA
MLBr_02546|M.leprae_Br4923 LEDIEKHQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGA
. :.*.*: :: *. : : . : :
MSMEG_3694|M.smegmatis_MC2_155 DLLRRAIEALG-VEFHQGYGMTETGGNVTFLGPDDHRAGAA----GHPEV
TH_3281|M.thermoresistible__bu GLIRRAIELLD-VDFHQGYGMTETAGNVTFLGPDDHRS-------DDPEI
MAB_4559|M.abscessus_ATCC_1997 AVLQRATRVFPNARFTQAYGMTEVSPVATLLLPDDHDD---------PAL
MMAR_0466|M.marinum_M PLVRRALELLPDVGFVNAYGLTETSSTIAVLTPDDHRAAQSAAAAGLARR
MUL_1115|M.ulcerans_Agy99 PLVRRALELLPDVGFVNAYRLTETSSTIAVLTPDDHRAAQSAAAAGLARR
MAV_1843|M.avium_104 PLVRKALDLLPHVGFVNAYGLTETSSTIAVLTPDDHREAYGNPDEAVAKR
Mflv_2385|M.gilvum_PYR-GCK PLVRKALSLLPDVGFVNAYGLTETSSTIAVLTPDDHRAALAAADDAALRR
Mvan_4263|M.vanbaalenii_PYR-1 PLVRKALSLLPAVGFVNAYGLTETSSTIAVLTPDDHRAALAASDEAAIRR
Mb0276|M.bovis_AF2122/97 ELATRALGDLGPVIYN-MYGSTEVAFATIAGPKD------------LQFN
Rv0270|M.tuberculosis_H37Rv ELATRALGDLGPVIYN-MYGSTEVAFATIAGPKD------------LQFN
MLBr_02546|M.leprae_Br4923 ELATRALEELGPVIYN-MYGSTEIAFATIAGPKD------------LQIN
: :* : . : * ** . *
MSMEG_3694|M.smegmatis_MC2_155 LVSAGHPHSGVEIGIVDADGAPLPPGGIGEIVVRGAQVARGYWPD----R
TH_3281|M.thermoresistible__bu LASAGRPHDGVRLRIVDPHGVEVAPGGIGEIQVRGEQVCS----------
MAB_4559|M.abscessus_ATCC_1997 LRSAGRAAPHCEVRIVDPDDNEVPRGEIGEVIVKGDNVMLGYWELPEESA
MMAR_0466|M.marinum_M LGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGGQVSGRYTGIG---S
MUL_1115|M.ulcerans_Agy99 LGSVGRPVPGVELQIRGQDGRVLGPGETGELFVRGEQVSGRYTGIG---S
MAV_1843|M.avium_104 LGSVGRPVPGVEVQIRDESGRVLGPGEPGELYVRGEQVSGKYTGIG---S
Mflv_2385|M.gilvum_PYR-GCK LGSVGQPVPGIEVEVRAEDGTVLGPGETGELFVRGEQVSGKYTDIG---S
Mvan_4263|M.vanbaalenii_PYR-1 LGSVGQPVPGIEVEIRADDGTVLGAGETGELFVRGEQVSGKYTDIG---S
Mb0276|M.bovis_AF2122/97 PSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGGG---G
Rv0270|M.tuberculosis_H37Rv PSTVGPVVKGVTVKILDENGNEVPQGAVGRIFVGNAFPFEGYTGGG---G
MLBr_02546|M.leprae_Br4923 SATVGPVVKGVKVKILDDSGKEVPRGQVGRIFVGNAFPFEGYTGGG---G
:.* : : . : * *.: * .
MSMEG_3694|M.smegmatis_MC2_155 PSTVDGWLHTGDIGRLDHTGRLFVVDRLKDIIVTGGENVSSREVEDVLSG
TH_3281|M.thermoresistible__bu ----PGWLATGDVGRLDRTGRLTVVDRAKDIIITGGENVSSREVEDVLSS
MAB_4559|M.abscessus_ATCC_1997 AAIRDGWMHTGDAGRMDDRGYVYIVDRIKDMVVTGGENVYSAEVENALAK
MMAR_0466|M.marinum_M VLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLIE
MUL_1115|M.ulcerans_Agy99 VLDGHGWFGTKDIAMLDEDGYLFIGGRSDDTIIRGGENIAPAELEEVLIE
MAV_1843|M.avium_104 VLDDEGWFPTRDIATLDEAGYLFITGRADDTIIRGGENIAPAELEDVLIE
Mflv_2385|M.gilvum_PYR-GCK VLDENGWFPTKDVAYLDSEGYLFIGGRSDDTIIRGGENIAPAEIEDVLVE
Mvan_4263|M.vanbaalenii_PYR-1 VLDEQGWFPTKDVAFLDEEGYLFIGGRSDDTIIRGGENIAPAEIEDVLVE
Mb0276|M.bovis_AF2122/97 KQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVEDLISG
Rv0270|M.tuberculosis_H37Rv KQIIDGLLSSGDVGYFDERGLLYVSGRDDEMIVSGGENVFPAEVEDLISG
MLBr_02546|M.leprae_Br4923 KQIIDGLLSSGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISG
* : : * . :* . : : .* .: :: ****: . *:*: :
MSMEG_3694|M.smegmatis_MC2_155 HPDVDMVAVVGVPDGYWGEAVCAVVVPVPGRNPRADDLIDHVRSAIAAFK
TH_3281|M.thermoresistible__bu HPGVDMCAVVAAPDEHWGEAVCAVVVPRRRPPPTADDLVAHVRSRLAAFK
MAB_4559|M.abscessus_ATCC_1997 HPAVAACAVIGIPDEQWGERVHAVVVKQAQSECCDGDLQEHCREHIANYK
MMAR_0466|M.marinum_M HPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSLRGSR
MUL_1115|M.ulcerans_Agy99 HPQVRDVAVVGVEDAQWGQEIVAVVVPVAGVDPDPEELREFVRRSLRGSR
MAV_1843|M.avium_104 HPHVHEVAVVGVDDPQWGQAIVAVIVPVPGVTPDPDELRDYAHKYLRGSR
Mflv_2385|M.gilvum_PYR-GCK HPHVRDCAVVGADDPQWGQIIVAVVVPHQDTAPDPEDLRAHVRSQLRGSR
Mvan_4263|M.vanbaalenii_PYR-1 HPHVRDCAVVGADDPEWGQIIVAVVVAQPGTEPDVEDLRSHVRSQLRGSR
Mb0276|M.bovis_AF2122/97 HPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHLARYK
Rv0270|M.tuberculosis_H37Rv HPDVVEAAAIGVDDKEFGARLRAFVVKKPGADLDEDTIKQYVRDHLARYK
MLBr_02546|M.leprae_Br4923 HPEVVEATAIGVDDKEWGARLRAFVVKKQDATIDEDAIKAYVRDHLARYK
** * :.:. * :* : *.:* : . : : :
MSMEG_3694|M.smegmatis_MC2_155 RPREVLFVDELPLTGNGKIAKDRVRDIARTALTA-------
TH_3281|M.thermoresistible__bu RPRHVLFVDELPMTPNGKVAKDEVRRHVRERLTPHTLENRT
MAB_4559|M.abscessus_ATCC_1997 VPRSFEFVDELPLSGAGKILKRVLRQKYWESRDAGVS----
MMAR_0466|M.marinum_M TPDRVVFRHELPTTATGKILRREIIEELAGTKS--------
MUL_1115|M.ulcerans_Agy99 TPDRVVFRRELPTTATGKILRREIIEELAGTKS--------
MAV_1843|M.avium_104 TPDRVVFRDELPTNATGKLLRREIVDSLSATMAEK------
Mflv_2385|M.gilvum_PYR-GCK TPDRVVFRDELPTNATGKVLRRELVSELNQDRSPAQPAV--
Mvan_4263|M.vanbaalenii_PYR-1 TPDRVVFRDELPTNATGKVLRRELVDELNQDPSPAQPAV--
Mb0276|M.bovis_AF2122/97 VPREVIFLDELPRNPTGKVLKRELRKL--------------
Rv0270|M.tuberculosis_H37Rv VPREVIFLDELPRNPTGKVLKRELRKL--------------
MLBr_02546|M.leprae_Br4923 VPREVIFLEELPRNPTGKVLKRELRDFEVH-----------
* . * *** . **: : :