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RTTKSHVIALLAELHHSGDHEAAWRLPTGRPELISDYGHYLSLAREAERGGFDALFFADFQAFREGFRDR MPWGFDPISLLAAIAADCPEIGIVATASTVFNRADSLARTFATLHQQTSGRVGWNIVTSGTPEAAASFGL STNPPHDERYRQAEDFVLNVLDWWRKDPNNIGLSAENRPALVQAGASPVGRDFAARYAELVFTAVPDVRA AADFRADIRRRASRIGRSPNDVKVTPGFFVVVGSTEAEAKALREELDAKLTDNQLRRMLADFGIDIDRYG LDDEIQGHLRDLTGSHSGIQSRIEVFQRVASSMGSEVTLRKLARRVAGSRGHLSCTGTPEQIADTMERVL DAGGADGFVIKFSHNPGGITDFVDGVIPELQRRGLYRGGYESQSLRARFGSNPHIANTFQQRAATGAALT AEKHHV*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4085 | - | - | 100% (427) | nitrilotriacetate monooxygenase component A |
| M. smegmatis MC2 155 | MSMEG_1162 | - | 7e-71 | 37.02% (443) | nitrilotriacetate monooxygenase component A |
| M. smegmatis MC2 155 | MSMEG_4096 | ssuD | 5e-65 | 36.79% (386) | nitrilotriacetate monooxygenase component A |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. gilvum PYR-GCK | Mflv_0250 | - | 3e-40 | 29.53% (403) | hypothetical protein Mflv_0250 |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4232c | - | 3e-47 | 31.54% (428) | putative oxygenase |
| M. marinum M | MMAR_0703 | - | 1e-40 | 30.43% (414) | FMNH2-utilizing oxygenase |
| M. avium 104 | MAV_3425 | - | 2e-58 | 35.37% (410) | nitrilotriacetate monooxygenase component A |
| M. thermoresistible (build 8) | TH_4079 | - | 6e-68 | 37.38% (428) | PUTATIVE nitrilotriacetate monooxygenase component A |
| M. ulcerans Agy99 | MUL_4507 | fgd2 | 2e-06 | 22.61% (314) | F420-dependent glucose-6-phosphate dehydrogenase Fgd2 |
| M. vanbaalenii PYR-1 | Mvan_6020 | - | 7e-52 | 31.18% (449) | xenobiotic compound DszA family monooxygenase |
CLUSTAL 2.0.9 multiple sequence alignment
TH_4079|M.thermoresistible__bu ----------------LPETRLSG--------------------ITDIDH
Mvan_6020|M.vanbaalenii_PYR-1 --------------MMHLITWLMGNSYHAAGWRHQLAWERT---AMRLDV
MAB_4232c|M.abscessus_ATCC_199 MSNPADAVRTREGKDRKPVHLAAHFPGVNNTTVWSDPASGS---QIDFDS
MMAR_0703|M.marinum_M -------------MIRKPVHLAAHFPGVNNTTVWADPGAGS---QIEFES
MAV_3425|M.avium_104 --------MSRRERGLTLNVNVLNLGIHTASWRRSAEPPTA---FTDPGY
MSMEG_4085|M.smegmatis_MC2_155 -------MRTTKSHVIALLAELHHSGDHEAAWRLPTGRPEL---ISDYGH
Mflv_0250|M.gilvum_PYR-GCK -------MTDSAKRKAHLLGFVQHGVMNHASTMWAHPRDKVGYHWSRPEY
MUL_4507|M.ulcerans_Agy99 -------------MALLFHWFLPTYGDSRGLVAGGHGLPMSGNRPATLRY
TH_4079|M.thermoresistible__bu YLELARIAERGKLDAIFFADHPVLKGKTEFRPFDA---------LDPVTL
Mvan_6020|M.vanbaalenii_PYR-1 LIEIAKIAEEAKLDALFVADGNGVRNMDKVGLFEANTPSARPTVYDPVTL
MAB_4232c|M.abscessus_ATCC_199 FVHLARTAERGLFDFFFLAEGLRLR-EHRERIYDLDVVGR----PDTFTV
MMAR_0703|M.marinum_M FVHLARTAERGLFDFFFLAEGLRLR-EHRGRIHDLDVVGR----PDTFTL
MAV_3425|M.avium_104 YVRMARIAERGTLDALFLADGPAVRDHPALRPSQA---------LEPSVI
MSMEG_4085|M.smegmatis_MC2_155 YLSLAREAERGGFDALFFADFQAFREGFRDRMPWG---------FDPISL
Mflv_0250|M.gilvum_PYR-GCK WRDLGRTMERGLFDAMFIADELAPYTTYKGSSDPVVKFAGQCPVHEPASV
MUL_4507|M.ulcerans_Agy99 LNQIAAAAESNGFEAVLTPAGLWCEDAWLTT-------------------
:. * :: .: .
TH_4079|M.thermoresistible__bu VTALSTATTHIGLVATASTTYTEPYGLARRFATLDHVSRGRAGWNIVTTA
Mvan_6020|M.vanbaalenii_PYR-1 MAAISQHTKHIGLVGTASTTYESPWVVARRFASLDHLSNGRASWNVVTGS
MAB_4232c|M.abscessus_ATCC_199 LAALGAVTERLGLVGTINTTFNEPFNVARQFASLDHLSDGRAGWNMVTSS
MMAR_0703|M.marinum_M LAALAGVTDRIGLTGTINTTFNEPFEVARQFATLDHLSEGRAGWNIVTSS
MAV_3425|M.avium_104 LAAVAAETEHLGLIGTFSSTFNDPVELAHRLLSLDQLSGGRLAWNIVTTY
MSMEG_4085|M.smegmatis_MC2_155 LAAIAADCPEIGIVATASTVFNRADSLARTFATLHQQTSGRVGWNIVTSG
Mflv_0250|M.gilvum_PYR-GCK VPIVGAFTKHLGIGITLSTSFVPPYMMARQLSTLDHLTGGRVGWNIVTSY
MUL_4507|M.ulcerans_Agy99 -AMLVGTTKTLKFLVALRPGLVSPTLAAQMATTFQQQSGGRLLVNVVTGG
. : : : : . . *: ::.: : ** *:**
TH_4079|M.thermoresistible__bu NAAAAANFGRPAHPDPEHRYSRADEFLQVALRLWDSWEDDAIVGDKQAPR
Mvan_6020|M.vanbaalenii_PYR-1 NPGDSENFGLAHHPDRDSRYARAEEFVSACKALWNSWDEDAFVERKDTGQ
MAB_4232c|M.abscessus_ATCC_199 DAFTGENFRRGGFLDHADRYRRAEEFITVARKFWDSWDAAG---------
MMAR_0703|M.marinum_M DAFTGANFRRGGYLDHADRYQRAEEFLTVARKFWDSWAPGAVLADIENGD
MAV_3425|M.avium_104 SEAAGGNFGLADLPDRSTRYRRAAEFVDVALALWRDAATGG---------
MSMEG_4085|M.smegmatis_MC2_155 TPEAAASFGLSTNPPHDERYRQAEDFVLNVLDWWR---------------
Mflv_0250|M.gilvum_PYR-GCK SKSEFQAMGKENLTPRDKRYEVVEEYMQLLYQLWDSWDDDAIVYDRDNGV
MUL_4507|M.ulcerans_Agy99 EAHEQRAYG--DFLDKQQRYRRTGEFLHVVRQLWASGQP-----------
** . ::: *
TH_4079|M.thermoresistible__bu FVDPDKIHAIDHRGEHFSVAGPLEVPRSPQGHPVLFQAGSSEPGKQLAAK
Mvan_6020|M.vanbaalenii_PYR-1 YLNARKVRVPDYKGRHLSVKGPLNVSRSPQGRPVLFHAGQSEGGRRLAAR
MAB_4232c|M.abscessus_ATCC_199 --EP---LPVNHRGPQFTVRGVATAPASPQRHPVLLQAGDSADGRDFGAK
MMAR_0703|M.marinum_M YVDPERIRSVEHSGPQFEVRGFATLPAGPQGHPVLLQAGDSEQGRAFGAR
MAV_3425|M.avium_104 --------GVDHRGEFFAVTGSLPQGPSPQGHPLLVQAGGSPQGRELAGR
MSMEG_4085|M.smegmatis_MC2_155 -------------------KDPNNIGLSAENRPALVQAGASPVGRDFAAR
Mflv_0250|M.gilvum_PYR-GCK FADPAKVREVNFQGEFFQSRGRHFVAPSPQKRPVLWQAGSSAQGREFASR
MUL_4507|M.ulcerans_Agy99 ---------VTFEGEHIHVHQAQ-LGRLPDPPPPVFFGGSSESAGAVAAR
.: * : .* * . ...:
TH_4079|M.thermoresistible__bu YAEAIFTAQPTVEEARQFYADVKARVRQHGRDPDRVHILPGLSAIIGGTE
Mvan_6020|M.vanbaalenii_PYR-1 HADCIFEAAASVEAAREFYADLKRRRVEIGGEPDHLRIIVSVAVYLGRTE
MAB_4232c|M.abscessus_ATCC_199 HADALFTIHSSIPAGQKYYADVKARAVAHGRNPDQLKVFPGVTYVLGDTE
MMAR_0703|M.marinum_M HADAMFTLHSSLRDGQRYYADVKGRARSYGRDPDQLKVFPAATFVIGDTP
MAV_3425|M.avium_104 VADAVFSAELDLDAAIEHYRYVKDAAVAHGRGRDAVRILPGLITTIGSSR
MSMEG_4085|M.smegmatis_MC2_155 YAELVFTAVPDVRAAADFRADIRRRASRIGRSPNDVKVTPGFFVVVGSTE
Mflv_0250|M.gilvum_PYR-GCK HAESVFGIFPTPKSMRAYATDIRTRASDAGRDPESVKLIYGLQTIIDRDK
MUL_4507|M.ulcerans_Agy99 YADTYLTWGEPLSAVGKKIDWIRGLAADHGR---ALSYGLRIHVITRDTA
*: : :: * :
TH_4079|M.thermoresistible__bu EEARRIQRRLED-LTVPDYGLEQLSRIVGYRVGRDDLDRRLQFGSDDPPA
Mvan_6020|M.vanbaalenii_PYR-1 SEAVELYSELNS-LISPDLGVDFLAKAVSEDLTGYPVDGPMPDLS--APV
MAB_4232c|M.abscessus_ATCC_199 QEAIEKAAYIRDQQVGPQTAIAYLEQVWGRDLSEYDPEGPLPDVDPTGDA
MMAR_0703|M.marinum_M DQAGEKARQISYQQVSGATAIAMLEQVWGRDLSDYDPDGPLPDIDPIADA
MAV_3425|M.avium_104 AEAQRRYEEQNA-LLPAGHDVERLSQTLGADLSGLPLDEPIPARLLGSVP
MSMEG_4085|M.smegmatis_MC2_155 AEAKALREELDAKLTDN--QLRRMLADFGIDIDRYGLDDEIQGHLRDLTG
Mflv_0250|M.gilvum_PYR-GCK GRANDRYAEFVENVQIES-ALGILSGHTGFDFSTLGLDDNVVDADVQGIR
MUL_4507|M.ulcerans_Agy99 EEAWRVADRLLAGIDPAD------IERMQANLARSESEGQRRMAELHG--
.* . :
TH_4079|M.thermoresistible__bu IHMQS---------RYALVRRLVSTENLTVRELLLRLSGGRGHRVFAGTP
Mvan_6020|M.vanbaalenii_PYR-1 VGGNS---------IRGQLDAIAKAEQLTVRQMYERVVPTMGNTALIGTA
MAB_4232c|M.abscessus_ATCC_199 SITRGKARHGDPREIAAKYREIAEAKKLSIRELIKEVTS---RQQFIGTP
MMAR_0703|M.marinum_M AISQGRVRHADPVAVARRYRERSQAENLSIRELVIAVTS---RQQFVGTP
MAV_3425|M.avium_104 DPSKFVGSLG----FRESVVRLVVSGGLTLRQTLRAFSGS-GHRVIVGSP
MSMEG_4085|M.smegmatis_MC2_155 SHSGIQSR-----IEVFQRVASSMGSEVTLRKLARRVAGSRGHLSCTGTP
Mflv_0250|M.gilvum_PYR-GCK G-------------LFDAILEAKDGAPVTVREAAQIYGVSMGAPVAVGTP
MUL_4507|M.ulcerans_Agy99 ----------------GVLDQLEIAPNLWAGVGLVRGGAG---TALVGSH
: *:
TH_4079|M.thermoresistible__bu EQVADTIEEWFTTGAADGFNLIPPALPSSLTAFVDHVVPELQRRGLFRTE
Mvan_6020|M.vanbaalenii_PYR-1 TQIADVMEEWYTTGACDGFVLGASISPFTLLLIRDELVPELQRRGLFRRH
MAB_4232c|M.abscessus_ATCC_199 SQVATEVDRYIQADACDGFILVPHLTPGGLDDFVDTVVPLLQERGAFRAE
MMAR_0703|M.marinum_M SHIADEIDRYVQADACDGFILVPHLTPHGLDEFVDRVVPLLQERGAFRTA
MAV_3425|M.avium_104 VDVADTIEQWFGAGAADGFNLMPDVFPDGLEVFVDEVVPILRSRGLFRAS
MSMEG_4085|M.smegmatis_MC2_155 EQIADTMERVLDAGGADGFVIKFSHNPGGITDFVDGVIPELQRRGLYRGG
Mflv_0250|M.gilvum_PYR-GCK ADVADQMEQYLDEGDCNGFMMLATDTPGCFNDMTELLVPELQKRGRFRTR
MUL_4507|M.ulcerans_Agy99 QEVAERIIEYARLG-ISHFILSGYPHLEEAYWFGEGVLPILERKRLWHRP
.:* : . . . * : : : ::* *. : ::
TH_4079|M.thermoresistible__bu YTGTTLRDHLGLPRPRNRFTTGRPVAAAS--------
Mvan_6020|M.vanbaalenii_PYR-1 YTGSTLRENLGLPPVDN-----SPFAT----------
MAB_4232c|M.abscessus_ATCC_199 YEHETLREHLGLDPISRASGDFRRETASA--------
MMAR_0703|M.marinum_M YTGQTLRDHLGLAGLPS--------------------
MAV_3425|M.avium_104 YAEATLRERLTGDRHVVAA------------------
MSMEG_4085|M.smegmatis_MC2_155 YESQSLRARFGSNPHIANTFQQRAATGAALTAEKHHV
Mflv_0250|M.gilvum_PYR-GCK YPGTTLRESLREY------------------------
MUL_4507|M.ulcerans_Agy99 HGSSPHSAALPVAHSSVN-------------------
: . :