For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. smegmatis MC2 155 MSMEG_3470 (-)

annotation: NAD-dependent epimerase/dehydratase
coordinates: 3536879 - 3537790
length: 303

MRVFVTGASGFIGSAVADELIAAGHTVIGLARSDASAEALTRAGHEVHRGDLTDLDSLRAGAASADGVIH
LAFVHDFENFAGAVQTDSAAIEALGETLAGSDRPLVVAAGVAGLPRGTTEDDAIPAGFPRLSETTALNQV
SKGVRASVVRLPPSVHGRGDYGFVPRLISIARERGVSGYPGDGSNAWSAVHRVDAARVFRLALEDAPAGS
RWHAVADEGIAVREIAETIGRGLGLPAVSVPPDQATAHFGWLGGFFAVDANLGSALTRQRLGWEPTGPGL
LADLDAGHYFESAAGQGLETVTG
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_3470--100% (303)NAD-dependent epimerase/dehydratase
M. smegmatis MC2 155MSMEG_5228-7e-0934.78% (115) 3-beta hydroxysteroid dehydrogenase/isomerase family protein
M. smegmatis MC2 155MSMEG_5799-1e-0830.92% (152) nucleoside-diphosphate-sugar epimerase

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97Mb3658cgalE11e-0826.06% (307) UDP-glucose 4-epimerase GALE1 (galactowaldenase)
M. gilvum PYR-GCKMflv_2075-1e-0731.06% (132) 3-beta hydroxysteroid dehydrogenase/isomerase
M. tuberculosis H37RvRv3634cgalE11e-0826.06% (307) UDP-glucose 4-epimerase GALE1 (galactowaldenase)
M. leprae Br4923MLBr_00204rmlB23e-0826.38% (307) putative sugar-nucleotide dehydratase
M. abscessus ATCC 19977MAB_0994-1e-10767.58% (293) putative NAD-dependent epimerase/dehydratase
M. marinum MMMAR_5133galE14e-0925.74% (303) UDP-glucose 4-epimerase GalE1
M. avium 104MAV_0588-9e-7552.53% (297) oxidoreductase
M. thermoresistible (build 8)TH_1114-8e-0833.85% (130) PROBABLE CHOLESTEROL DEHYDROGENASE
M. ulcerans Agy99MUL_4210galE13e-0925.74% (303) UDP-glucose 4-epimerase GalE1
M. vanbaalenii PYR-1Mvan_1926-8e-9460.62% (292) NAD-dependent epimerase/dehydratase

CLUSTAL 2.0.9 multiple sequence alignment


MMAR_5133|M.marinum_M               ----------MRALVTGAAGFIGSTLVDRLLADGHTVVGLDNFATGRATN
MUL_4210|M.ulcerans_Agy99           ----------MRALVTGAAGFIGSTLVDRLLADGHTVVGLDNFATGRATN
Mb3658c|M.bovis_AF2122/97           ----------MRALVTGAAGFIGSTLVDRLLADGHSVVGLDNFATGRATN
Rv3634c|M.tuberculosis_H37Rv        ----------MRALVTGAAGFIGSTLVDRLLADGHSVVGLDNFATGRATN
MLBr_00204|M.leprae_Br4923          -----MYGEPVRALVTGAAGFIGSTLVDRLLADGHTVVGLDNFATGHAAN
Mflv_2075|M.gilvum_PYR-GCK          ----MLRTELGRVLVTGGSGFVGANLVTELLDRGLHVRSFDRVAS-----
TH_1114|M.thermoresistible__bu      MGDPTLRTDLGRVLVTGGSGFVGTNLVTTLLERGHHVRSCDRVGS-----
MSMEG_3470|M.smegmatis_MC2_155      ----------MRVFVTGASGFIGSAVADELIAAGHTVIGLARSDAS----
MAB_0994|M.abscessus_ATCC_1997      ----------MRVFVTGASGFIGSAVVRELIDSGHTVTGLARSDSS----
Mvan_1926|M.vanbaalenii_PYR-1       ----------MKVFVTGASGFIGSAVTAELLGHGHHVLGLARSDAA----
MAV_0588|M.avium_104                ----------MRVLVTGASGGIGSAVVKELLAAGHHVIGLARSEAS----
                                               :.:***.:* :*: :.  *:  *  * .  .  :     

MMAR_5133|M.marinum_M               LEHLVDNLAHVFVEADIVDADLQAIFEQHRPEVIFHLAAQIDVR----HS
MUL_4210|M.ulcerans_Agy99           LEHLVDNLAHVFVEADIVDADLQAIFEQHRPEVIFHLAAQIDVR----HS
Mb3658c|M.bovis_AF2122/97           LEHLADNSAHVFVEADIVTADLHAILEQHRPEVVFHLAAQIDVR----RS
Rv3634c|M.tuberculosis_H37Rv        LEHLADNSAHVFVEADIVTADLHAILEQHRPEVVFHLAAQIDVR----RS
MLBr_00204|M.leprae_Br4923          LEHLASTPALAFVEADIVTADLQTILDEHRPEVVFHLAAQIDVR----HS
Mflv_2075|M.gilvum_PYR-GCK          --ALPAHARLEIVEGDITDADDVAAAVEG-VDTVFHTAAIIDLMGGASVS
TH_1114|M.thermoresistible__bu      --PLPDHPRLEVIDGDICDADAVAAAVDG-IDTVFHTAAVIDLMGGAGVT
MSMEG_3470|M.smegmatis_MC2_155      -AEALTRAGHEVHRGDLTDLDSLRAGAAS-ADGVIHLAFVHDFE------
MAB_0994|M.abscessus_ATCC_1997      -AAALTAAGADVHRGSLDDLESLRAGAVA-ADGVAHLAFVHDFE------
Mvan_1926|M.vanbaalenii_PYR-1       -AQRITATGAEAVRGDLDDLDALEAGASA-ADAVIHLAFQHDFT------
MAV_0588|M.avium_104                -AATVSGLGAEPLRGDIADLDVLQKAAVD-TDGVAYLAFSHDFS------
                                                  ..:   :          : : : *   *.       

MMAR_5133|M.marinum_M               VADPQFDASVNVIGTLRLAEAARLTGVRKVVHTSSGGSIYGTPPQYPTSE
MUL_4210|M.ulcerans_Agy99           VADPQFDASVNVIGTLRLAEAARLTGVRKVVHTSSGGSIYGTPPQYPTSE
Mb3658c|M.bovis_AF2122/97           VADPQFDAAVNVIGTVRLAEAARQTGVRKIVHTSSGGSIYGTPPEYPTPE
Rv3634c|M.tuberculosis_H37Rv        VADPQFDAAVNVIGTVRLAEAARQTGVRKIVHTSSGGSIYGTPPEYPTPE
MLBr_00204|M.leprae_Br4923          VVDPQFDASVNVIGTVRLAEAARHTGVRKIVHTSSGGSIYGTPSQYPTPE
Mflv_2075|M.gilvum_PYR-GCK          EEYRQRSFAVNVTGTQNLVHAAQKAGVTRFVYTASNSVVMGGQRIAGGDE
TH_1114|M.thermoresistible__bu      DEYRERSFAVNVHGTERLVRAAQQAGVRRFVYTASNSVVMGGQPIADGDE
MSMEG_3470|M.smegmatis_MC2_155      ----NFAGAVQTDSAAIEALGETLAGSDRPLVVAAGVA--GLPRGTTEDD
MAB_0994|M.abscessus_ATCC_1997      ----NYAEAVNADKRAIATIGEALAGTERPFVVTSGLAGFGLGRPLTEED
Mvan_1926|M.vanbaalenii_PYR-1       ----AYDTAARVDRQAIETLGEALAGSDRALVVASGMAGLGRGRILTEDQ
MAV_0588|M.avium_104                ----DVGDAIADEARAIDALGAALADTGKPLVLASGTP-ARPGSVSTEDD
                                            :         . .   :.  : .  ::.             :

MMAR_5133|M.marinum_M               RVPTDP--ASPYAAGKVAGEIYLNTFRHLYGLECSHIAPANVYGPRQDPH
MUL_4210|M.ulcerans_Agy99           RVPTDP--ASPYAAGKVAGEIYLNTFRHLYGLECSHIAPANVYGPRQDPH
Mb3658c|M.bovis_AF2122/97           TAPTDP--ASPYAAGKVAGEIYLNTFRHLYGLDCSHIAPANVYGPRQDPH
Rv3634c|M.tuberculosis_H37Rv        TAPTDP--ASPYAAGKVAGEIYLNTFRHLYGLDCSHIAPANVYGPRQDPH
MLBr_00204|M.leprae_Br4923          TVPTDP--TSPYAAGKVAGEIYLNTFRHLCGLDCSHIAPANVYGPRQDPY
Mflv_2075|M.gilvum_PYR-GCK          TLPYTERFNDLYTETKVVAEKFVLSQNGVSGLLTCSIRPSGIWGR-----
TH_1114|M.thermoresistible__bu      TLPYTDRFNDLYTETKVIAERFVLGQNGIEGMLTCSIRPSGIWGP-----
MSMEG_3470|M.smegmatis_MC2_155      AIPAGF---PRL----SETTALNQVSKGVRASVVRLPPSVHGRGD-----
MAB_0994|M.abscessus_ATCC_1997      AAAPES---PRL----SEE-ALNLADRGVRVSAVRLAPSVHGEGD-----
Mvan_1926|M.vanbaalenii_PYR-1       AAPPDA---PRE----SEPAVFAFTDRGVRAAAVRLAPTVHGEGD-----
MAV_0588|M.avium_104                PFIADG---PLAGRGRTGQAVVELAGRGVRSAVVRLPRAVHDAGGR----
                                                              . :         .    *      

MMAR_5133|M.marinum_M               GEAGVVAIFAQALLSGKPTKVFGDGTNTRDYVFVDDVVDAFVRAGSDVG-
MUL_4210|M.ulcerans_Agy99           GEAGVVAIFAQALLSGKPTKVFGDGTNTRDYVFVDDVVDAFVKAGSDVG-
Mb3658c|M.bovis_AF2122/97           GEAGVVAIFAQALLSGKPTRVFGDGTNTRDYVFVDDVVDAFVRVSADVG-
Rv3634c|M.tuberculosis_H37Rv        GEAGVVAIFAQALLSGKPTRVFGDGTNTRDYVFVDDVVDAFVRVSADVG-
MLBr_00204|M.leprae_Br4923          GEAGVVAIFVQALLSDRPTKVFGDGTHTRDYVFVDDVVDAFIKASGDAG-
Mflv_2075|M.gilvum_PYR-GCK          GDQTMFRKVFESVLAGHVKVLVGNKETKLDNSYVHNLVHGFILAAEHLVE
TH_1114|M.thermoresistible__bu      GDQTMFRKLFESVHAGHVKVLIGSRHARLDNSYVHNLVHGFILAAEHLVP
MSMEG_3470|M.smegmatis_MC2_155      --YGFVPRLISIARERGVSGYPGDGSNAWSAVHRVDAARVFRLALEDAP-
MAB_0994|M.abscessus_ATCC_1997      --HGFVPRLIEIARAKGVSAYPGDGSNRWPAVHRLDAAHLFRLALENAP-
Mvan_1926|M.vanbaalenii_PYR-1       --HGFVPTLISIARATGVSTYPGDGSNRWPAVHRLDAAALFRIAAEDAP-
MAV_0588|M.avium_104                --YGLAGILIQLARQRGVSSFAGDGTQRWPAVHRDDAAALFRLALEQAP-
                                        .   . .       .   *.        .  : .  *  .  .   

MMAR_5133|M.marinum_M               ----GGQRFNIGTGVETSDRQLHSAVAAAVG-------------------
MUL_4210|M.ulcerans_Agy99           ----GGQRFNIGTGVETSDRQLHSAVAAAVG-------------------
Mb3658c|M.bovis_AF2122/97           ----GGLRFNIGTGKETSDRQLHSAVAAAVG-------------------
Rv3634c|M.tuberculosis_H37Rv        ----GGLRFNIGTGKETSDRQLHSAVAAAVG-------------------
MLBr_00204|M.leprae_Br4923          ----GGQRFNIGTGIETSDRQLHTAVSAAVG-------------------
Mflv_2075|M.gilvum_PYR-GCK          GGSAPGQAYFINDGEPINMFEFARPVVQACGEPFPKFRVPGRLVWFAMTI
TH_1114|M.thermoresistible__bu      GGTAPGQAYFINDGEPINMFEFARPVVTACGQRWPTLRVSGPLVRAVMSL
MSMEG_3470|M.smegmatis_MC2_155      ----AGSRWHAVADEGIAVREIAETIGRGLGLP-----------------
MAB_0994|M.abscessus_ATCC_1997      ----TGSRLHGVAEDGIPVRDIAEVIGRHLNLP-----------------
Mvan_1926|M.vanbaalenii_PYR-1       ----AGTLLHAVADEGIPVRQIAEAIGHGLDVS-----------------
MAV_0588|M.avium_104                ----AGSVLHAVGDEGVPLRAIAEVIGRRLGVP-----------------
                                         *               :   :    .                   

MMAR_5133|M.marinum_M               --------GPDDPEFHPPRLGDLKRS-CLDISRAEEVLGWRPQVELADGV
MUL_4210|M.ulcerans_Agy99           --------GPDDPEFHPPRLGDLKRS-CLDISRAEEVLGWRPQVELADGV
Mb3658c|M.bovis_AF2122/97           --------GPDDPEFHPPRLGDLKRS-CLDIGLAERVLGWRPQIELADGV
Rv3634c|M.tuberculosis_H37Rv        --------GPDDPEFHPPRLGDLKRS-CLDIGLAERVLGWRPQIELADGV
MLBr_00204|M.leprae_Br4923          --------GPDDPEFHPPRLGDLKRS-CLDIGLATTVLGWSPQVQLDDGV
Mflv_2075|M.gilvum_PYR-GCK          WQFLHFKFGLPKPLLEPLAVERLYLDNYFSIAKAQRDLGYQPLFTTEQAL
TH_1114|M.thermoresistible__bu      WQRLHFRFGLPKPPLEPLAVERLYLDNYFSIDKARRELGYRPRYTTEQAL
MSMEG_3470|M.smegmatis_MC2_155      ---AVSVPPDQATAHFGWLGGFFAVDANLGSALTRQRLGWEPTGPGLLAD
MAB_0994|M.abscessus_ATCC_1997      ---VESVPLAQAFDHFGWLGGFFAMDLPATSVLTQERLGWRPVQPGLIAD
Mvan_1926|M.vanbaalenii_PYR-1       ---VEATTAQASIDRAGFIGAVFAMDLPASSALTRTQFGWQPTHPGLLAD
MAV_0588|M.avium_104                ---VESAP----ADAFGPLGQVFAVDQPSSSALTQRRFGWQPVGPGLLDD
                                                          :  .       :   :*: *        

MMAR_5133|M.marinum_M               RRTVDYFRRTHGS--------------------
MUL_4210|M.ulcerans_Agy99           RRTVDYFRRTHGS--------------------
Mb3658c|M.bovis_AF2122/97           RRTVEYFRHKHTD--------------------
Rv3634c|M.tuberculosis_H37Rv        RRTVEYFRHKHTD--------------------
MLBr_00204|M.leprae_Br4923          RRTVEYFRAAQRS--------------------
Mflv_2075|M.gilvum_PYR-GCK          AQCIPYYVELFDRMKREAGNPVVQAAAPAPPKG
TH_1114|M.thermoresistible__bu      QECLPYYVDLFHRMKAEPATV------------
MSMEG_3470|M.smegmatis_MC2_155      LDAGHYFESAAGQGLETVTG-------------
MAB_0994|M.abscessus_ATCC_1997      LEKGHYFDE------------------------
Mvan_1926|M.vanbaalenii_PYR-1       LSQAHYFATPEA---------------------
MAV_0588|M.avium_104                LETGVYPE-------------------------
                                         *