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MADSTTDLGRILVTGGSGFVGANLVTELLDRGYAVRSFDRVPSPLPAHAGLEVATGDICDLDNVTNAVAG VDTVFHTAAIIDLMGGASVTAEYRQRSFAVNVGGTENLVRAAQSAGVKRFVYTASNSVVMGGQHIVHGDE TLPYTERFNDLYTETKVVAEKFVLGRNGVAGMLTCSIRPSGIWGRGDQTMFRKVFESVLAGHVKVLVGRK STLLDNSYVHNLVHGFILAAEHLTPNGTAPGQAYFINDGEPVNMFEFARPVIEACGRKLPRVRVPGRAVH AAMSGWQRLHFRFGIPEPLLEPLAVERLYLNNYFSIAKATRDLGYRPLFTTEQARVDCLPYYVDLFKQME AQARPA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5228 | - | - | 100% (356) | 3-beta hydroxysteroid dehydrogenase/isomerase family protein |
| M. smegmatis MC2 155 | MSMEG_2247 | - | 1e-17 | 27.06% (340) | dihydrokaempferol 4-reductase |
| M. smegmatis MC2 155 | MSMEG_5956 | - | 5e-14 | 26.39% (269) | NAD dependent epimerase/dehydratase family protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1136c | - | 1e-162 | 77.90% (353) | cholesterol dehydrogenase |
| M. gilvum PYR-GCK | Mflv_2075 | - | 1e-163 | 80.75% (348) | 3-beta hydroxysteroid dehydrogenase/isomerase |
| M. tuberculosis H37Rv | Rv1106c | - | 1e-162 | 77.90% (353) | cholesterol dehydrogenase |
| M. leprae Br4923 | MLBr_01942 | - | 1e-153 | 73.11% (357) | putative cholesterol dehydrogenase |
| M. abscessus ATCC 19977 | MAB_0718c | - | 7e-18 | 25.23% (333) | NAD-dependent epimerase/dehydratase |
| M. marinum M | MMAR_4359 | - | 1e-159 | 77.34% (353) | cholesterol dehydrogenase |
| M. avium 104 | MAV_1225 | - | 1e-167 | 81.09% (349) | 3-beta hydroxysteroid dehydrogenase/isomerase family protein |
| M. thermoresistible (build 8) | TH_1114 | - | 1e-158 | 77.12% (354) | PROBABLE CHOLESTEROL DEHYDROGENASE |
| M. ulcerans Agy99 | MUL_0172 | - | 1e-159 | 77.05% (353) | cholesterol dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_4635 | - | 1e-163 | 81.02% (353) | 3-beta hydroxysteroid dehydrogenase/isomerase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2075|M.gilvum_PYR-GCK --------MLRTELGRVLVTGGSGFVGANLVTELLDRGLHVRSFDRVASA
Mvan_4635|M.vanbaalenii_PYR-1 ---MGDATTPTTELGRVLVTGGSGFVGANLVTELLDRGLQVRSFDRVPSP
MSMEG_5228|M.smegmatis_MC2_155 ------MADSTTDLGRILVTGGSGFVGANLVTELLDRGYAVRSFDRVPSP
Mb1136c|M.bovis_AF2122/97 MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSL
Rv1106c|M.tuberculosis_H37Rv MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSL
MAV_1225|M.avium_104 MIGPMGDPSLTAELGRVLVTGGSGFVGANLVTTLLERGYQVRSFDRAPSP
MMAR_4359|M.marinum_M MLRPMGDASLTTELGRVLVTGGSGFVGSNLVTTLLDRGHHVRSFDRAPSP
MUL_0172|M.ulcerans_Agy99 MLRPMGDASLTTELGRVLVTGGSGFVGSNLVTTLLDRGHHVRSFDRAPSP
MLBr_01942|M.leprae_Br4923 MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMP
TH_1114|M.thermoresistible__bu ----MGDPTLRTDLGRVLVTGGSGFVGTNLVTTLLERGHHVRSCDRVGSP
MAB_0718c|M.abscessus_ATCC_199 --------------MKTAVTGAAGFIGTNLVNLLVDNGHEVIAIDRVVPA
: ***.:**:*:***. *::.* * : **.
Mflv_2075|M.gilvum_PYR-GCK LPAH-ARLEIVEGDITDADDVAAAVEG----VDTVFHTAAIIDLMGGASV
Mvan_4635|M.vanbaalenii_PYR-1 LPDH-PGLEVVQGDITDVDDVARAVGTGADKADTVFHTAAIIDLMGGASV
MSMEG_5228|M.smegmatis_MC2_155 LPAH-AGLEVATGDICDLDNVTNAVAG----VDTVFHTAAIIDLMGGASV
Mb1136c|M.bovis_AF2122/97 LPAH-PQLEVLQGDITDADVCAAAVDG----IDTIFHTAAIIELMGGASV
Rv1106c|M.tuberculosis_H37Rv LPAH-PQLEVLQGDITDADVCAAAVDG----IDTIFHTAAIIELMGGASV
MAV_1225|M.avium_104 LPAH-PHLEVLQGDITDAGVCAAAVEG----IDTIFHTAAIIDLMGGASV
MMAR_4359|M.marinum_M LPPH-PQLEVLQGDITDAATCATAVDG----VDTVFHTAAIIELMGGASV
MUL_0172|M.ulcerans_Agy99 LPPH-PQLEVLQGDITDAATCATAVDG----VDTVFHTAAIIELMGGASV
MLBr_01942|M.leprae_Br4923 LPQH-PQLEVLQGDITDATVCTTAMDS----IDTVFHTAAIIELMGGASV
TH_1114|M.thermoresistible__bu LPDH-PRLEVIDGDICDADAVAAAVDG----IDTVFHTAAVIDLMGGAGV
MAB_0718c|M.abscessus_ATCC_199 SPETREAVTWVSGDVLDPDSMTKALDG----VEVVYHLVAVITLK-----
* : **: * : *: :.::* .*:* *
Mflv_2075|M.gilvum_PYR-GCK SEEYRQRSFAVNVTGTQNLVHAAQKAGVTRFVYTASNSVVMGGQRIAGGD
Mvan_4635|M.vanbaalenii_PYR-1 TEEYRQRSFAVNVTGTKNLVHAAQKAGVQRFVYTASNSVVMGGQRIAGGD
MSMEG_5228|M.smegmatis_MC2_155 TAEYRQRSFAVNVGGTENLVRAAQSAGVKRFVYTASNSVVMGGQHIVHGD
Mb1136c|M.bovis_AF2122/97 TDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGD
Rv1106c|M.tuberculosis_H37Rv TDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNSVVMGGQNIAGGD
MAV_1225|M.avium_104 TEEYRQRSFAVNVGGTENLVRAGQAAGVQRFVYTSSNSVVMGGQNIVGGD
MMAR_4359|M.marinum_M TDEYRKRSFSVNVGGTENLVREGQQAGVKRFVYTSSNSVVMGGQIIAGGD
MUL_0172|M.ulcerans_Agy99 TDEYRKRSFSVNVGGTENLVRAGQQAGVKRFVYTSSNSVVMGGQIIAGGD
MLBr_01942|M.leprae_Br4923 TDEYRQRSYTVNVGGTENLLRAGQKSGVKRFVYTASNSVVMGGTPITGGD
TH_1114|M.thermoresistible__bu TDEYRERSFAVNVHGTERLVRAAQQAGVRRFVYTASNSVVMGGQPIADGD
MAB_0718c|M.abscessus_ATCC_199 --HEDELCWRINTEGARTVAQAALTVGARRMVHCSS--IDSYSNSVATID
. : .: :*. *:. : : . *. *:*: :* : . :. *
Mflv_2075|M.gilvum_PYR-GCK ETLPYTERFN-DLYTETKVVAEKFVLSQNGV---SGLLTCSIRPSGIWGR
Mvan_4635|M.vanbaalenii_PYR-1 ETLPYTERFN-DLYTETKVVAEKFVLSQNGV---SGMLTCSIRPSGIWGR
MSMEG_5228|M.smegmatis_MC2_155 ETLPYTERFN-DLYTETKVVAEKFVLGRNGV---AGMLTCSIRPSGIWGR
Mb1136c|M.bovis_AF2122/97 ETLPYTDRFN-DLYTETKVVAERFVLAQNGV---DGMLTCAIRPSGIWGN
Rv1106c|M.tuberculosis_H37Rv ETLPYTDRFN-DLYTETKVVAERFVLAQNGV---DGMLTCAIRPSGIWGN
MAV_1225|M.avium_104 ETLPYTDRFN-DLYTETKVLAERFVLGQNGV---DGMLTCAIRPSGIWGR
MMAR_4359|M.marinum_M ETLPYTNRFN-DLYTETKVVAEKFVLSQNGN---NEMLTCAIRPSGIWGT
MUL_0172|M.ulcerans_Agy99 ETLPYTNRFN-DLYTETKVVAEKFVLSQNGN---NEMLTCAIRPSGIWGT
MLBr_01942|M.leprae_Br4923 ETMPYTKRFN-DLYTETKVVAEKFVLSQNGVPDGETMLTCSIRPSGIWGR
TH_1114|M.thermoresistible__bu ETLPYTDRFN-DLYTETKVIAERFVLGQNGI---EGMLTCSIRPSGIWGP
MAB_0718c|M.abscessus_ATCC_199 EKSPRSAAADLPVYQRSKWGGEVAVRETIAS----GLDAVICNPTGVYGP
*. * : : :* .:* .* * . : : .*:*::*
Mflv_2075|M.gilvum_PYR-GCK GDQTMFRKVFESVLAGHVKVLVGNKETKLDNSYVHNLVHGFILAAEHLVE
Mvan_4635|M.vanbaalenii_PYR-1 GDQTMFRKVFESVLAGHVKVLVGNKNVKLDNSYVHNLVHGFILAAQHLVD
MSMEG_5228|M.smegmatis_MC2_155 GDQTMFRKVFESVLAGHVKVLVGRKSTLLDNSYVHNLVHGFILAAEHLTP
Mb1136c|M.bovis_AF2122/97 GDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVP
Rv1106c|M.tuberculosis_H37Rv GDQTMFRKLFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVP
MAV_1225|M.avium_104 GDQTMFRKLFESVIAGHVKVLIGRKSARLDNSYVHNLIHGFILAAQHLTP
MMAR_4359|M.marinum_M GDQLMFRKLFESVIKGHVKVLVGPKSALLDNSYVDNLIHGFILAAQHLVP
MUL_0172|M.ulcerans_Agy99 GDQLMFRKLFESVIKGHVKVLVGPKSVLLDNSYVDNLIHGFMLAAQHLVP
MLBr_01942|M.leprae_Br4923 GDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAEHLVP
TH_1114|M.thermoresistible__bu GDQTMFRKLFESVHAGHVKVLIGSRHARLDNSYVHNLVHGFILAAEHLVP
MAB_0718c|M.abscessus_ATCC_199 VDLPNLSRINQLLFDSARGRLPGMVNSQYDLVDARDVAAGLYLAGEKGR-
* : : : : . * * * . :: *: **. :
Mflv_2075|M.gilvum_PYR-GCK GGSAPGQAYFINDGEPINMFEFARPVVQACGEPFPKFRVPGRLVWFAMTI
Mvan_4635|M.vanbaalenii_PYR-1 GGTAPGQAYFINDGEPINMFEFARPVMEACGEPWPTFRVSGRLVWFAMTI
MSMEG_5228|M.smegmatis_MC2_155 NGTAPGQAYFINDGEPVNMFEFARPVIEACGRKLPRVRVPGRAVHAAMSG
Mb1136c|M.bovis_AF2122/97 DGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTG
Rv1106c|M.tuberculosis_H37Rv DGTAPGQAYFINDAEPINMFEFARPVLEACGQRWPKMRISGPAVRWVMTG
MAV_1225|M.avium_104 GGTAPGQAYFINDAEPINMFEFARPVVEACGVNWPRVRVNGPIVRAAMTG
MMAR_4359|M.marinum_M GGSAPGQAYFINDAEPINMFEFARPVVEACGEKWPRVRVSGPMVHRAMTG
MUL_0172|M.ulcerans_Agy99 GGSAPGQAYFINDAEPINMFDFARPVVEACGEKWPRVRVSGPMVHRAMTG
MLBr_01942|M.leprae_Br4923 GGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVMAV
TH_1114|M.thermoresistible__bu GGTAPGQAYFINDGEPINMFEFARPVVTACGQRWPTLRVSGPLVRAVMSL
MAB_0718c|M.abscessus_ATCC_199 ----TGENYLLG-GHMGSLLQVCRLAARRAGKRGPRFAVPMGLLNAAIPV
.*: *::. .. .:::. .* * . : : .:.
Mflv_2075|M.gilvum_PYR-GCK WQFLHFKFGLPKPLLEPLAVERLYLDNYFSIAKAQRDLGYQPLFTTEQAL
Mvan_4635|M.vanbaalenii_PYR-1 WQFLHFRFGLPKPLLEPLAVERLYLDNYFSIAKAERDLGYRPLYTTEQAL
MSMEG_5228|M.smegmatis_MC2_155 WQRLHFRFGIPEPLLEPLAVERLYLNNYFSIAKATRDLGYRPLFTTEQAR
Mb1136c|M.bovis_AF2122/97 WQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQAL
Rv1106c|M.tuberculosis_H37Rv WQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGYEPLFTTQQAL
MAV_1225|M.avium_104 WQRLHFRFGIPAPLLEPLAVERLYLDNFFSIAKASRDLGYQPLFTTEQAM
MMAR_4359|M.marinum_M WQRLHFRFGLPAPLLEPLAVERLYLDNYFSVDKARRDLGYEPKFTTEQAL
MUL_0172|M.ulcerans_Agy99 WQRLHFRFGLPAPLLEPLAVERLYLDNYFSVDKARRDLGYEPKFTTEQAL
MLBr_01942|M.leprae_Br4923 WQRLHFGFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGYRPLFTTEQAM
TH_1114|M.thermoresistible__bu WQRLHFRFGLPKPPLEPLAVERLYLDNYFSIDKARRELGYRPRYTTEQAL
MAB_0718c|M.abscessus_ATCC_199 IEPIGKLLKN--DALSRAAFNKLTVSPAVDITKARTELGYEPR-STETTV
: : : :. *.::: :. ..: ** :***.* :*: :
Mflv_2075|M.gilvum_PYR-GCK AQCIPYYVELFDRMKREAGNPVVQAAAPAPPKG
Mvan_4635|M.vanbaalenii_PYR-1 EHCIPYYVDLFGRMKAEAAQPAVQAAAPVPPAG
MSMEG_5228|M.smegmatis_MC2_155 VDCLPYYVDLFKQMEAQAR-PA-----------
Mb1136c|M.bovis_AF2122/97 TECLPYYVSLFEQMKNEARAEKTAATVKP----
Rv1106c|M.tuberculosis_H37Rv TECLPYYVSLFEQMKNEARAEKTAATVKP----
MAV_1225|M.avium_104 SECLPYYVGMFEQMKRQALAGKASA--------
MMAR_4359|M.marinum_M KECLPYYVDLFRQMKSEVKAGGR----------
MUL_0172|M.ulcerans_Agy99 KECLPYYVDLFRQMKSEVKAGGR----------
MLBr_01942|M.leprae_Br4923 AECLPYYTELFEQIKVAAKPHLASIAATPRPE-
TH_1114|M.thermoresistible__bu QECLPYYVDLFHRMKAEPATV------------
MAB_0718c|M.abscessus_ATCC_199 NEFVDFLLGSGRL--------------------
. : :