For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
TEHLTVGALIRHHAADTPDKPMVVDPKERIAYGDLDEKTRAMASGLVAAGVGKGSRVGLLMPNGTDWVLT ALAVTRIGAILVPLSTMLTPTELEAQLRTASVQFLIAVDEFRGRGYPVAPERLPALRQMWTAEQALTMTG EPASADALAARVRPSDPMVILFTSGSSGPPKGVIHSHGNAVRAVASGLAARCVTADTRLYLPMPFFWVGG FGAGIVTALVAGATLVTEPIPSPQSTLDLLHRERVTLFRGWPDQAEALARHASSTDLSALRPGSLEALLP HELRAQPGARASLFGMTESFGPYCGYPADADMPRSAWGSCGRPFDSMDVRIVDTTTGTPLRVDEVGEIQI RGPHILRGICRRGREEVFTRDGYYPTGDLGRLDSDGFLFYHGRSDDMFKVSGATVYPGEAQHALRGIAGV RAAYVVNVPDHQRNRVGAVVVTDGMSARDLCAAAREVLSPFKVPSVWALLDDEAAVPIGATGKVDNNGLR AMLAERGER*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2215 | - | - | 100% (500) | AMP-binding enzyme |
| M. smegmatis MC2 155 | MSMEG_2216 | - | 8e-80 | 36.71% (493) | AMP-binding enzyme |
| M. smegmatis MC2 155 | MSMEG_3468 | - | 8e-61 | 35.46% (533) | AMP-binding enzyme |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3591 | fadD3 | 4e-51 | 31.27% (518) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_4364 | - | 0.0 | 67.27% (498) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 9e-51 | 31.27% (518) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 8e-27 | 30.39% (464) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4377c | - | 3e-42 | 29.92% (518) | AMP-binding domain protein |
| M. marinum M | MMAR_4027 | - | 1e-170 | 60.96% (502) | fatty-acid-CoA ligase |
| M. avium 104 | MAV_1577 | - | 1e-177 | 64.29% (504) | AMP-binding enzyme, putative |
| M. thermoresistible (build 8) | TH_4352 | - | 1e-119 | 70.72% (304) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 3e-50 | 30.74% (501) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_1980 | - | 1e-176 | 62.98% (497) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3591|M.bovis_AF2122/97 ----------MINDLRTVPAALDRLVRQLPDHTALIAE--DRRFTSTELR
Rv3561|M.tuberculosis_H37Rv ----------MINDLRTVPAALDRLVRQLPDHTALIAE--DRRFTSTELR
MUL_4126|M.ulcerans_Agy99 ----------MTSDPRTVPTALDRLVRRLPNHDALVTD--ERSFTVSELN
MAB_4377c|M.abscessus_ATCC_199 MKAYDAGATLSPIIEETIGENFERIARAHPDVGALVDVSGDRRWTYRELD
MMAR_4027|M.marinum_M ---------MPD----TIDHLVRSQAARHGAKPMVIDQ--ASRLSYRELD
MAV_1577|M.avium_104 ---------MPEASTPTIDRLVRSRAAEFGGKPMVIDP--GYRITYDQLD
TH_4352|M.thermoresistible__bu --------------------------------------------------
Mflv_4364|M.gilvum_PYR-GCK ---------MTD----TVDALLRAQAERHPDTEAVVDP--AERITYRELD
Mvan_1980|M.vanbaalenii_PYR-1 ---------MTD----TIDSLLRDQAAAHGARDAVVDP--AERISYAELN
MSMEG_2215|M.smegmatis_MC2_155 ---------MTEH--LTVGALIRHHAADTPDKPMVVDP--KERIAYGDLD
MLBr_01051|M.leprae_Br4923 ----------------------MLLASLNPSVVTATDIADAVCVDGVVLS
Mb3591|M.bovis_AF2122/97 DAVYGAAAALIALGVEPADRVAIWSPNTWHWVVACLAIHHAGAAVVPLNT
Rv3561|M.tuberculosis_H37Rv DAVYGAAAALIALGVEPADRVAIWSPNTWHWVVACLAIHHAGAAVVPLNT
MUL_4126|M.ulcerans_Agy99 DEVYRAAAALIELGVKPAARVGIWSPNTWHWVVACLAIHHVGAAMVPLNT
MAB_4377c|M.abscessus_ATCC_199 AEINRVARGLMSLGVAAGDRVGIWAPNCAEWVLVQYATAKIGAILVNINP
MMAR_4027|M.marinum_M SSTSDLAAAFIQAGAGKGTRVGLIMPNGVRWVQIAIALTRIGAVLVPLST
MAV_1577|M.avium_104 TATRELAAVFVQAGVGKGTRVGLIMPNNTRWVLIAIALTRIGAVLVPLST
TH_4352|M.thermoresistible__bu --------------------------------------------------
Mflv_4364|M.gilvum_PYR-GCK AATRELGAAFVASGITKGTRVGLLMPNGVEWARTALALMRIGAVLVPLST
Mvan_1980|M.vanbaalenii_PYR-1 RSTGELGAAFVASGIGKGMRVALLMPNGVAWARIALALMRFGAVVVPLST
MSMEG_2215|M.smegmatis_MC2_155 EKTRAMASGLVAAGVGKGSRVGLLMPNGTDWVLTALAVTRIGAILVPLST
MLBr_01051|M.leprae_Br4923 RSGLVGAATSVAERVGGARLVAVLATPTASTVLAITGCLIAGVPVVPVPA
Mb3591|M.bovis_AF2122/97 RYTATEATDILDRAGAPVLFAAGLFLGADRAAGLDRAALPALRHVVRVPV
Rv3561|M.tuberculosis_H37Rv RYTATEATDILDRAGAPVLFAAGLFLGADRAAGLDRAALPALRHVVRVPV
MUL_4126|M.ulcerans_Agy99 RYTADEAADILARTEAPVLFAMGHFLGSDRVAGLDRKALPALRHIVRIPI
MAB_4377c|M.abscessus_ATCC_199 AYRTHELAYALNQSGVRTLICAKAFKSSDYVA-MASQVMPDAPGLRDVVF
MMAR_4027|M.marinum_M LLRPRELASQLRSAAVQFLISVPEFRGHRYLDAVESVPRAELPALQRVWA
MAV_1577|M.avium_104 LLRAGELVAQLRVAAVQFLVSVDEFRGHRYLDDVAAV-RSELPALQQVWP
TH_4352|M.thermoresistible__bu --------------------------------------------------
Mflv_4364|M.gilvum_PYR-GCK LLTPPELDAQLRTASVQHLIAVEEFRGHRYLDRLDAD----LPALSTVWT
Mvan_1980|M.vanbaalenii_PYR-1 LLTARELSAQLQTASVQHLIAVEEFRGHRY--HLDAT----PPALRSVRT
MSMEG_2215|M.smegmatis_MC2_155 MLTPTELEAQLRTASVQFLIAVDEFRGRGYPVAPERL-----PALRQMWT
MLBr_01051|M.leprae_Br4923 DIGVVERRHMLTDS------GAQAWLGPGPSADSAPDGLPHIPVQLDARS
Mb3591|M.bovis_AF2122/97 EADDGTWDEFIATGAGALDAVAARAAAVAPQDVSDILFTSGTTGRSKGVL
Rv3561|M.tuberculosis_H37Rv EADDGTWDEFIATGAGALDAVAARAAAVAPQDVSDILFTSGTTGRSKGVL
MUL_4126|M.ulcerans_Agy99 EEPDGTWDEFIETGT-DTGAVTARAAAVSPDDVSDILFTSGTTGRSKGVL
MAB_4377c|M.abscessus_ATCC_199 IGTSDWTELVAGAERVTETALRARMSQLSNTDPINIQYTSGTTGYPKGAT
MMAR_4027|M.marinum_M DDQ----VSSSNAGARALRMVEPIAETVTAADTLVIMFTSGSTGAPKGVL
MAV_1577|M.avium_104 TEQ----LDAAAAGARAGQIVDAMTQTVTPADPLVIMFTSGSSGTPKGVW
TH_4352|M.thermoresistible__bu --------------------------------------------------
Mflv_4364|M.gilvum_PYR-GCK TDQ----ITGLRPGPGTAAVSDAMSRGVRAADPMAILFTSGSSGPPKGVI
Mvan_1980|M.vanbaalenii_PYR-1 PEQ----VFALRGHPAAAEVAEAMSRGVRPADPLTVVFTSGSSGAPKGVR
MSMEG_2215|M.smegmatis_MC2_155 AEQ----ALTMTGEPAS---ADALAARVRPSDPMVILFTSGSSGPPKGVI
MLBr_01051|M.leprae_Br4923 WNR---------------------YPEPSPDATAMVIYTSGTTGPPKGVL
Mb3591|M.bovis_AF2122/97 CAHRQSLSASASWAANGKITSDDRYLCINPFFHNFGYKAGILACLQTGAT
Rv3561|M.tuberculosis_H37Rv CAHRQSLSASASWAANGKITSDDRYLCINPFFHNFGYKAGILACLQTGAT
MUL_4126|M.ulcerans_Agy99 CAHRQSLSASASWAANGKITADDRHLCINPFFHNFGYKAGILACLQTGAT
MAB_4377c|M.abscessus_ATCC_199 LSHRNVLNNGFFVAESIQLQAGDRLCIPVPFYHCFGMVMGNLGCTTHGAT
MMAR_4027|M.marinum_M HSHGSALGAVRSGLTARRITSATRLYLPMPFFWVGGFGGGILSALLAGAT
MAV_1577|M.avium_104 HSHGSALGAVQSGLAARCIDADSRLYLPMPFFWVGGFGSGILSALLAGAT
TH_4352|M.thermoresistible__bu ------------------------LYLPMPFFWVGGFGGGVLSALVAGAT
Mflv_4364|M.gilvum_PYR-GCK HSHGNAIGAVRSGLDARCIDDRTRLYLPMPFFWVGGFGGGLLSALVAGAT
Mvan_1980|M.vanbaalenii_PYR-1 HSHGNAIGAVRSGLAARCVDADTRLYLPMPFFWIGGLGGGLLSALTAGAT
MSMEG_2215|M.smegmatis_MC2_155 HSHGNAVRAVASGLAARCVTADTRLYLPMPFFWVGGFGAGIVTALVAGAT
MLBr_01051|M.leprae_Br4923 LSRRAIAVDLDALAQAWQWTADDVLVHGLPLFHVHGLVLGLLGSLRIGNR
*:: * * : . *
Mb3591|M.bovis_AF2122/97 LIPHVT-FDPLHALRAIERHRITVLPGPPTIYQSLLDHPARKDFDLSSLR
Rv3561|M.tuberculosis_H37Rv LIPHVT-FDPLHALRAIERHRITVLPGPPTIYQSLLDHPARKDFDLSSLR
MUL_4126|M.ulcerans_Agy99 LFPHLT-FDPLRTLQAIERHRITVLPGPPTIYQTLLDHPARDDYDLSSLR
MAB_4377c|M.abscessus_ATCC_199 IVMPAPGFDPGRTLEAIERERCVGVYGVPTMFIAMLADPGFAHRDLSTLR
MMAR_4027|M.marinum_M LVTEAR-PFPENTLRLLEAERVTMFRGWPDQAEALARHPSSVDVDLSALQ
MAV_1577|M.avium_104 LVTEEI-PRPETTLRLLESERVTLFRGWPDQAETLARHAGTVGADLSALR
TH_4352|M.thermoresistible__bu LVTEEK-PQPHTTLRLLERERVTLFRGWPDQAEALARHRESVGADLSSLR
Mflv_4364|M.gilvum_PYR-GCK LVTEAV-PQPDSTLRLLTAERVTLFRGWPDQAEALARHLPASGVELN-LR
Mvan_1980|M.vanbaalenii_PYR-1 LITEPV-PQPDSTLRLLADERVTLFRGWPDQAEALARHLPASGVELTELR
MSMEG_2215|M.smegmatis_MC2_155 LVTEPI-PSPQSTLDLLHRERVTLFRGWPDQAEALARHASST--DLSALR
MLBr_01051|M.leprae_Br4923 FVHTGK-PTPTAYAQACSEAGGSLYFGVPTVWSRLVADEAVAR-ALRPAR
:. * * * : . * :
Mb3591|M.bovis_AF2122/97 FAVTGAATVPVVLVERMQSELDIDIVLTAYGLTEANGMGTMCRPEDDAV-
Rv3561|M.tuberculosis_H37Rv FAVTGAATVPVVLVERMQSELDIDIVLTAYGLTEANGMGTMCRPEDDAV-
MUL_4126|M.ulcerans_Agy99 FAVTGAATVPVVLVERMQSELDIDIVLTAYGLTEANGMGTMCRADDDAL-
MAB_4377c|M.abscessus_ATCC_199 TGIMAGSVCPVEVMKRCIDEMHMAGVAIAYGMTETSPVSCQTLFDDDLD-
MMAR_4027|M.marinum_M PGSLPALLPPERRAQ-------PGARARLFGMTEAFGPYCGYRADTDMP-
MAV_1577|M.avium_104 PGSLQALLPPEQRAR-------PGARATLFGMTEAFGPYSGYPADTDMP-
TH_4352|M.thermoresistible__bu PGSLEALLPPDQRAD-------RGARANLFGMTESFGPYCGYRADTDMP-
Mflv_4364|M.gilvum_PYR-GCK PGSLEALLPPNLRAR-------PGARANLFGMTESFGPYCGHPADTDMP-
Mvan_1980|M.vanbaalenii_PYR-1 AGSLDALLPPRLRSR-------PGARARLFGMTESFGPYCGYPADTDMP-
MSMEG_2215|M.smegmatis_MC2_155 PGSLEALLPHELRAQ-------PGARASLFGMTESFGPYCGYPADADMP-
MLBr_01051|M.leprae_Br4923 LLVSGSASLPVPVFDR-------LAHLTGHRPIERYGSTESLITLSTLAD
. . *
Mb3591|M.bovis_AF2122/97 -TVAT-TCGRPFADFELRIADD------------GEVLLRGPNVMVGYLD
Rv3561|M.tuberculosis_H37Rv -TVAT-TCGRPFADFELRIADD------------GEVLLRGPNVMVGYLD
MUL_4126|M.ulcerans_Agy99 -TVAT-TCGRPFADFELRIGPDT-----------GEVLLRGPNVMLGYLD
MAB_4377c|M.abscessus_ATCC_199 -RRTA-TVGRAHPHIEIKIVDPNSG-ETVQRGQSGELCTRGYSVMLGYWN
MMAR_4027|M.marinum_M -PTAWGSCGKPFPGMEIRIVDPETG-TRVPAETIGQIQIRGPHTLRGLCR
MAV_1577|M.avium_104 -VSARGSCGKPFDGMEVRIVDPDTG-APVGAGTAGIIQIRGPHTLRGMCG
TH_4352|M.thermoresistible__bu -RSAWGSCGKPFPGMEVRIVDPDSG-APVPAGTVGEIQLRGPHLMRGICR
Mflv_4364|M.gilvum_PYR-GCK -ETAWGSCGTPFDGMQVRITDPGTG-EELPAGATGMIQIRGPHLMRGICR
Mvan_1980|M.vanbaalenii_PYR-1 -ETAWGSCGKPFDGMQVRIVGADTG-EPAPTGAIGMIQIRGRHVMRGICR
MSMEG_2215|M.smegmatis_MC2_155 -RSAWGSCGRPFDSMDVRIVDTTTG-TPLRVDEVGEIQIRGPHILRGICR
MLBr_01051|M.leprae_Br4923 GERRAGWVGLPLAGVQTRLVDESGGPVPYDGETVGRLQVRSPTMFGGYLN
* . .: :: * : *. : *
Mb3591|M.bovis_AF2122/97 DTEATAAAIDADGWLHTGDIGAVDQAGNLRINDR-LKDMYICGGFNVYPA
Rv3561|M.tuberculosis_H37Rv DTEATAAAIDADGWLHTGDIGAVDQAGNLRITDR-LKDMYICGGFNVYPA
MUL_4126|M.ulcerans_Agy99 DPRATAAAIDADGWLHTGDIGVLDEAGNLRITDR-LKDMYICGGFNVYPA
MAB_4377c|M.abscessus_ATCC_199 DEAHTREVLDTDGWMHTGDLAVMRDDGYCTIIGR-LKDMVIRGGENIYPR
MMAR_4027|M.marinum_M --RSREEVFTADGYYCSGDLGHLDANGYLFYHGR-SDDMFKVSGATVYPV
MAV_1577|M.avium_104 --RSREELFTVDGFYPTGDLGHLDDAGFLFYHGR-ADDMFKVSGATVYPS
TH_4352|M.thermoresistible__bu --RSREEVFTPDGFYPTGDLGRLDADGFLFYHGR-RDDMFKVSGATVYPG
Mflv_4364|M.gilvum_PYR-GCK --RTREDVFTPDGFYPTGDLGHLDSDGFLFYHGR-CDDMFKVRGATVYPA
Mvan_1980|M.vanbaalenii_PYR-1 --RSREEVFTSDGWYATGDLGHLDADGFLYYHGR-CDDMFKVRGATVYPS
MSMEG_2215|M.smegmatis_MC2_155 --RGREEVFTRDGYYPTGDLGRLDSDGFLFYHGR-SDDMFKVSGATVYPG
MLBr_01051|M.leprae_Br4923 RPEATAEAFDEDGWYRTGDVAVVDSGGMHRIVGRESVDLIKLGGYRIGAG
: **: :**:. : * .* *: * : .
Mb3591|M.bovis_AF2122/97 EVEQVLARMDGVADAAVIGVPDQRLGEVGRAFVVARPG-TGLDEASVIAY
Rv3561|M.tuberculosis_H37Rv EVEQVLARMDGVADAAVIGVPDQRLGEVGRAFVVARPG-TGLDEASVIAY
MUL_4126|M.ulcerans_Agy99 EVEQVLARLDGVADVAVIGIPDHRLGEVGRAFIVARPG-FNLDEKSVIDY
MAB_4377c|M.abscessus_ATCC_199 EIEEFLLTHPDIEDVHVVGVPDEKYGEELCAWVRMRPDRVVIDAVAIRAF
MMAR_4027|M.marinum_M EVETALRTIDGVSNAFVTNVA-GAQGQRVAAAVVCSTP--ALTSEDLRTS
MAV_1577|M.avium_104 EVERALRTIDGVDGAVVTNVP-GATGDRVGAAVVCR----ELTAAQLRAA
TH_4352|M.thermoresistible__bu EVADALRSIDGVRAAYVTDVADGRGGDRVGAAVVGT----VTDVGQLRRE
Mflv_4364|M.gilvum_PYR-GCK EVEQALRTVDGVRAASVTNLP-GPEGNRVGAAVVCD-A--SLTVDVLRRA
Mvan_1980|M.vanbaalenii_PYR-1 EVEQALRSLDGVSAAFVTNVA-GPEGDRVGAAVVCD-A--PATAERLRSA
MSMEG_2215|M.smegmatis_MC2_155 EAQHALRGIAGVRAAYVVNVP-DHQRNRVGAVVVTD----GMSARDLCAA
MLBr_01051|M.leprae_Br4923 EIEMALLGHPDVREVAVVGLPDEDLGQRIVAFVVGAEA---LDADELINY
* * .: . * .:. : * : :
Mb3591|M.bovis_AF2122/97 TREHLANFKTPRSVRFVDV---LPRNAAGKVSKPQLRELG----------
Rv3561|M.tuberculosis_H37Rv TREHLANFKTPRSVRFVDV---LPRNAAGKVSKPQLRELG----------
MUL_4126|M.ulcerans_Agy99 TREHLANFKAPRSVRFVDT---LPRNAGGKVVKPQLRELA----------
MAB_4377c|M.abscessus_ATCC_199 ASGRLAHYKIPRYVHVVES---FPMTVTGKVRKIEMRQQTIQIFGLPEPG
MMAR_4027|M.marinum_M AGQVLSAFKVPTVWLLLADDANIPRGPTGKVDLQRLRDMLANTA------
MAV_1577|M.avium_104 ARNLLSSFKVPSVWLVLRSDDDLPRGVTGKVDVRRLRELLADADRRQETR
TH_4352|M.thermoresistible__bu ARKLLSSFKVPTVWLLLDSDDAVPRRATGKVDVNRLRDLLATHPDRVVT-
Mflv_4364|M.gilvum_PYR-GCK AREVLSSFKVPSVWALLPDEAAIPRGATGKVDTARLRELLQD--------
Mvan_1980|M.vanbaalenii_PYR-1 AREVLSSFKVPTVWLLLDSQDGVPRGATGKVDVTRLRALLEQS-------
MSMEG_2215|M.smegmatis_MC2_155 AREVLSPFKVPSVWALLDDEAAVPIGATGKVDNNGLRAMLAERGER----
MLBr_01051|M.leprae_Br4923 VAQQLSIHKRPREVRFVDA---LPRNAMGKVLKKQLLFEG----------
. *: .* * .: .* *** :
Mb3591|M.bovis_AF2122/97 -----
Rv3561|M.tuberculosis_H37Rv -----
MUL_4126|M.ulcerans_Agy99 -----
MAB_4377c|M.abscessus_ATCC_199 RTDEQ
MMAR_4027|M.marinum_M -----
MAV_1577|M.avium_104 VQG--
TH_4352|M.thermoresistible__bu -----
Mflv_4364|M.gilvum_PYR-GCK -----
Mvan_1980|M.vanbaalenii_PYR-1 -----
MSMEG_2215|M.smegmatis_MC2_155 -----
MLBr_01051|M.leprae_Br4923 -----