For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
MTDTVDALLRAQAERHPDTEAVVDPAERITYRELDAATRELGAAFVASGITKGTRVGLLMPNGVEWARTA LALMRIGAVLVPLSTLLTPPELDAQLRTASVQHLIAVEEFRGHRYLDRLDADLPALSTVWTTDQITGLRP GPGTAAVSDAMSRGVRAADPMAILFTSGSSGPPKGVIHSHGNAIGAVRSGLDARCIDDRTRLYLPMPFFW VGGFGGGLLSALVAGATLVTEAVPQPDSTLRLLTAERVTLFRGWPDQAEALARHLPASGVELNLRPGSLE ALLPPNLRARPGARANLFGMTESFGPYCGHPADTDMPETAWGSCGTPFDGMQVRITDPGTGEELPAGATG MIQIRGPHLMRGICRRTREDVFTPDGFYPTGDLGHLDSDGFLFYHGRCDDMFKVRGATVYPAEVEQALRT VDGVRAASVTNLPGPEGNRVGAAVVCDASLTVDVLRRAAREVLSSFKVPSVWALLPDEAAIPRGATGKVD TARLRELLQD
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4364 | - | - | 100% (500) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_4363 | - | 8e-81 | 38.13% (514) | AMP-dependent synthetase and ligase |
| M. gilvum PYR-GCK | Mflv_3245 | - | 5e-50 | 32.15% (535) | AMP-dependent synthetase and ligase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3591 | fadD3 | 2e-45 | 31.79% (519) | acyl-CoA synthetase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 4e-45 | 31.79% (519) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 5e-27 | 24.30% (502) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_0596c | - | 2e-41 | 28.05% (517) | fatty-acid-CoA ligase FadD |
| M. marinum M | MMAR_4027 | - | 0.0 | 65.28% (504) | fatty-acid-CoA ligase |
| M. avium 104 | MAV_1577 | - | 0.0 | 66.40% (503) | AMP-binding enzyme, putative |
| M. smegmatis MC2 155 | MSMEG_2215 | - | 0.0 | 67.27% (498) | AMP-binding enzyme |
| M. thermoresistible (build 8) | TH_4352 | - | 1e-124 | 74.25% (299) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 3e-44 | 31.29% (521) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_1980 | - | 0.0 | 76.80% (500) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3591|M.bovis_AF2122/97 ------------------------------------MINDLR----TVPA
Rv3561|M.tuberculosis_H37Rv ------------------------------------MINDLR----TVPA
MUL_4126|M.ulcerans_Agy99 ------------------------------------MTSDPR----TVPT
MAB_0596c|M.abscessus_ATCC_199 ------------------------------------MESAQQTPGLTIPA
Mflv_4364|M.gilvum_PYR-GCK ------------------------------------MTD-------TVDA
Mvan_1980|M.vanbaalenii_PYR-1 ------------------------------------MTD-------TIDS
MMAR_4027|M.marinum_M ------------------------------------MPD-------TIDH
MAV_1577|M.avium_104 ------------------------------------MPEAST---PTIDR
TH_4352|M.thermoresistible__bu --------------------------------------------------
MSMEG_2215|M.smegmatis_MC2_155 ------------------------------------MTEH-----LTVGA
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mb3591|M.bovis_AF2122/97 ALDRLVRQLPDHTALIAEDRRFTSTELRDAVYGAAAALIALGVEPADRVA
Rv3561|M.tuberculosis_H37Rv ALDRLVRQLPDHTALIAEDRRFTSTELRDAVYGAAAALIALGVEPADRVA
MUL_4126|M.ulcerans_Agy99 ALDRLVRRLPNHDALVTDERSFTVSELNDEVYRAAAALIELGVKPAARVG
MAB_0596c|M.abscessus_ATCC_199 ALDQAAARFGERDALYADGSWLSFSELRARARRFAAALIALGVEHGQRLA
Mflv_4364|M.gilvum_PYR-GCK LLRAQAERHPDTEAVVDPAERITYRELDAATRELGAAFVASGITKGTRVG
Mvan_1980|M.vanbaalenii_PYR-1 LLRDQAAAHGARDAVVDPAERISYAELNRSTGELGAAFVASGIGKGMRVA
MMAR_4027|M.marinum_M LVRSQAARHGAKPMVIDQASRLSYRELDSSTSDLAAAFIQAGAGKGTRVG
MAV_1577|M.avium_104 LVRSRAAEFGGKPMVIDPGYRITYDQLDTATRELAAVFVQAGVGKGTRVG
TH_4352|M.thermoresistible__bu --------------------------------------------------
MSMEG_2215|M.smegmatis_MC2_155 LIRHHAADTPDKPMVVDPKERIAYGDLDEKTRAMASGLVAAGVGKGSRVG
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEEGTLTYKELDRAAHALANGLIAKGVRGGDGIA
Mb3591|M.bovis_AF2122/97 IWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAA
Rv3561|M.tuberculosis_H37Rv IWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPVLFAA
MUL_4126|M.ulcerans_Agy99 IWSPNTWHWVVACLAIHHVGAAMVPLNTRYTADEAADILARTEAPVLFAM
MAB_0596c|M.abscessus_ATCC_199 IWSPNSWHWPIACLGGQYAGAAVVPMNTRYTVDEATDILQRSHARVLVSV
Mflv_4364|M.gilvum_PYR-GCK LLMPNGVEWARTALALMRIGAVLVPLSTLLTPPELDAQLRTASVQHLIAV
Mvan_1980|M.vanbaalenii_PYR-1 LLMPNGVAWARIALALMRFGAVVVPLSTLLTARELSAQLQTASVQHLIAV
MMAR_4027|M.marinum_M LIMPNGVRWVQIAIALTRIGAVLVPLSTLLRPRELASQLRSAAVQFLISV
MAV_1577|M.avium_104 LIMPNNTRWVLIAIALTRIGAVLVPLSTLLRAGELVAQLRVAAVQFLVSV
TH_4352|M.thermoresistible__bu --------------------------------------------------
MSMEG_2215|M.smegmatis_MC2_155 LLMPNGTDWVLTALAVTRIGAILVPLSTMLTPTELEAQLRTASVQFLIAV
MLBr_02546|M.leprae_Br4923 ILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVIIYD
Mb3591|M.bovis_AF2122/97 GLFLGADRAAGLDRAALPALRHVVRVPVEADDGT-WDEFIATGAG-ALDA
Rv3561|M.tuberculosis_H37Rv GLFLGADRAAGLDRAALPALRHVVRVPVEADDGT-WDEFIATGAG-ALDA
MUL_4126|M.ulcerans_Agy99 GHFLGSDRVAGLDRKALPALRHIVRIPIEEPDGT-WDEFIETGT--DTGA
MAB_0596c|M.abscessus_ATCC_199 GKFLGTDRIEQLDTSSLPDLRHIVRVAVEDGDESGWDAFVAHGDGVSDGD
Mflv_4364|M.gilvum_PYR-GCK EEFRGHRYLDRLDAD----LPALSTVWTTDQITG------LRPGPGTAAV
Mvan_1980|M.vanbaalenii_PYR-1 EEFRGHRY--HLDAT----PPALRSVRTPEQVFA------LRGHPAAAEV
MMAR_4027|M.marinum_M PEFRGHRYLDAVESVPRAELPALQRVWADDQVSS------SNAGARALRM
MAV_1577|M.avium_104 DEFRGHRYLDDVAAV-RSELPALQQVWPTEQLDA------AAAGARAGQI
TH_4352|M.thermoresistible__bu --------------------------------------------------
MSMEG_2215|M.smegmatis_MC2_155 DEFRGRGYPVAPERL-----PALRQMWTAEQALT------MTGEPAS---
MLBr_02546|M.leprae_Br4923 DEYTKVVSKAEPALG---KLRALGVNPDEEEPPG----STDETLAELIAR
Mb3591|M.bovis_AF2122/97 VAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKIT
Rv3561|M.tuberculosis_H37Rv VAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKIT
MUL_4126|M.ulcerans_Agy99 VTARAAAVSPDDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGKIT
MAB_0596c|M.abscessus_ATCC_199 IDTRKAAVSSEDISDILFTSGTTGRSKGVLCMHRQPLAASLAWATYGEFT
Mflv_4364|M.gilvum_PYR-GCK SDAMSRGVRAADPMAILFTSGSSGPPKGVIHSHGNAIGAVRSGLDARCID
Mvan_1980|M.vanbaalenii_PYR-1 AEAMSRGVRPADPLTVVFTSGSSGAPKGVRHSHGNAIGAVRSGLAARCVD
MMAR_4027|M.marinum_M VEPIAETVTAADTLVIMFTSGSTGAPKGVLHSHGSALGAVRSGLTARRIT
MAV_1577|M.avium_104 VDAMTQTVTPADPLVIMFTSGSSGTPKGVWHSHGSALGAVQSGLAARCID
TH_4352|M.thermoresistible__bu --------------------------------------------------
MSMEG_2215|M.smegmatis_MC2_155 ADALAARVRPSDPMVILFTSGSSGPPKGVIHSHGNAVRAVASGLAARCVT
MLBr_02546|M.leprae_Br4923 SSSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFR
Mb3591|M.bovis_AF2122/97 SDDRYLCINPFFHNFGYKAGILACLQTGATLIPHVTFDPLHALRAIERHR
Rv3561|M.tuberculosis_H37Rv SDDRYLCINPFFHNFGYKAGILACLQTGATLIPHVTFDPLHALRAIERHR
MUL_4126|M.ulcerans_Agy99 ADDRHLCINPFFHNFGYKAGILACLQTGATLFPHLTFDPLRTLQAIERHR
MAB_0596c|M.abscessus_ATCC_199 ENDRYLCVNPFFHNFGYKAGILACLQTGAALMPQLTFDPEKAMAAVQEHK
Mflv_4364|M.gilvum_PYR-GCK DRTRLYLPMPFFWVGGFGGGLLSALVAGATLVTEAVPQPDSTLRLLTAER
Mvan_1980|M.vanbaalenii_PYR-1 ADTRLYLPMPFFWIGGLGGGLLSALTAGATLITEPVPQPDSTLRLLADER
MMAR_4027|M.marinum_M SATRLYLPMPFFWVGGFGGGILSALLAGATLVTEARPFPENTLRLLEAER
MAV_1577|M.avium_104 ADSRLYLPMPFFWVGGFGSGILSALLAGATLVTEEIPRPETTLRLLESER
TH_4352|M.thermoresistible__bu ----LYLPMPFFWVGGFGGGVLSALVAGATLVTEEKPQPHTTLRLLERER
MSMEG_2215|M.smegmatis_MC2_155 ADTRLYLPMPFFWVGGFGAGIVTALVAGATLVTEPIPSPQSTLDLLHRER
MLBr_02546|M.leprae_Br4923 AHEVTLLPAPMFHALGYLHATLAMFLG-STLVLRRRFKPATVLEDIEKHQ
*:* * . :: : ::*. . * .: : .:
Mb3591|M.bovis_AF2122/97 ITVLPGPPTIYQSLLD--HPARKDFDLSSLRFAVTGAATVPVVLVERMQS
Rv3561|M.tuberculosis_H37Rv ITVLPGPPTIYQSLLD--HPARKDFDLSSLRFAVTGAATVPVVLVERMQS
MUL_4126|M.ulcerans_Agy99 ITVLPGPPTIYQTLLD--HPARDDYDLSSLRFAVTGAATVPVVLVERMQS
MAB_0596c|M.abscessus_ATCC_199 ITVLPGAPTIFQMLLD--HPARKNYDLTSLRYSVTGAAVVPVVLIERMQS
Mflv_4364|M.gilvum_PYR-GCK VTLFRGWPDQAEALAR--HLPASGVELN-LRPGSLEALLPPNLRAR----
Mvan_1980|M.vanbaalenii_PYR-1 VTLFRGWPDQAEALAR--HLPASGVELTELRAGSLDALLPPRLRSR----
MMAR_4027|M.marinum_M VTMFRGWPDQAEALAR--HPSSVDVDLSALQPGSLPALLPPERRAQ----
MAV_1577|M.avium_104 VTLFRGWPDQAETLAR--HAGTVGADLSALRPGSLQALLPPEQRAR----
TH_4352|M.thermoresistible__bu VTLFRGWPDQAEALAR--HRESVGADLSSLRPGSLEALLPPDQRAD----
MSMEG_2215|M.smegmatis_MC2_155 VTLFRGWPDQAEALAR--HASST--DLSALRPGSLEALLPHELRAQ----
MLBr_02546|M.leprae_Br4923 ATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATR---
* : * . : . :*. *: .
Mb3591|M.bovis_AF2122/97 ELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVAT-TCGRPFADFELRIAD
Rv3561|M.tuberculosis_H37Rv ELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVAT-TCGRPFADFELRIAD
MUL_4126|M.ulcerans_Agy99 ELDIDIVLTAYGLTEANGMGTMCRADDDALTVAT-TCGRPFADFELRIGP
MAB_0596c|M.abscessus_ATCC_199 ELDFDLVLTAYGLTETQGFATICRSDDDAVTVAT-TCGRPMLGYELRIDG
Mflv_4364|M.gilvum_PYR-GCK ---PGARANLFGMTESFGPYCGHPADTDMPETAWGSCGTPFDGMQVRITD
Mvan_1980|M.vanbaalenii_PYR-1 ---PGARARLFGMTESFGPYCGYPADTDMPETAWGSCGKPFDGMQVRIVG
MMAR_4027|M.marinum_M ---PGARARLFGMTEAFGPYCGYRADTDMPPTAWGSCGKPFPGMEIRIVD
MAV_1577|M.avium_104 ---PGARATLFGMTEAFGPYSGYPADTDMPVSARGSCGKPFDGMEVRIVD
TH_4352|M.thermoresistible__bu ---RGARANLFGMTESFGPYCGYRADTDMPRSAWGSCGKPFPGMEVRIVD
MSMEG_2215|M.smegmatis_MC2_155 ---PGARASLFGMTESFGPYCGYPADADMPRSAWGSCGRPFDSMDVRIVD
MLBr_02546|M.leprae_Br4923 -ALEELGPVIYNMYGSTEIAFATIAGPKDLQINSATVGPVVKGVKVKILD
:.: : . . : * . . .::*
Mb3591|M.bovis_AF2122/97 D-----------GEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGDIGA
Rv3561|M.tuberculosis_H37Rv D-----------GEVLLRGPNVMVGYLDDTEATAAAIDADGWLHTGDIGA
MUL_4126|M.ulcerans_Agy99 DT----------GEVLLRGPNVMLGYLDDPRATAAAIDADGWLHTGDIGV
MAB_0596c|M.abscessus_ATCC_199 AEK-----PGDEGEVLLRSANVMKGYLDDPKATAEAIDDDGWLHTGDIGK
Mflv_4364|M.gilvum_PYR-GCK PGTGEELPAGATGMIQIRGPHLMRGICR--RTREDVFTPDGFYPTGDLGH
Mvan_1980|M.vanbaalenii_PYR-1 ADTGEPAPTGAIGMIQIRGRHVMRGICR--RSREEVFTSDGWYATGDLGH
MMAR_4027|M.marinum_M PETGTRVPAETIGQIQIRGPHTLRGLCR--RSREEVFTADGYYCSGDLGH
MAV_1577|M.avium_104 PDTGAPVGAGTAGIIQIRGPHTLRGMCG--RSREELFTVDGFYPTGDLGH
TH_4352|M.thermoresistible__bu PDSGAPVPAGTVGEIQLRGPHLMRGICR--RSREEVFTPDGFYPTGDLGR
MSMEG_2215|M.smegmatis_MC2_155 TTTGTPLRVDEVGEIQIRGPHILRGICR--RGREEVFTRDGYYPTGDLGR
MLBr_02546|M.leprae_Br4923 DS-GKEVPRGQVGRIFVGNAFPFEGYTG----GGGKQIIDGLLSSGDVGY
* : : . : * ** :**:*
Mb3591|M.bovis_AF2122/97 VDQAGNLRINDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAVIGVPDQ
Rv3561|M.tuberculosis_H37Rv VDQAGNLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAVIGVPDQ
MUL_4126|M.ulcerans_Agy99 LDEAGNLRITDRLKDMYICGGFNVYPAEVEQVLARLDGVADVAVIGIPDH
MAB_0596c|M.abscessus_ATCC_199 LDARGNLTITDRLKDMYICGGFNVYPAEIEQVLMRLDGVADVAVIGVPDA
Mflv_4364|M.gilvum_PYR-GCK LDSDGFLFYHGRCDDMFKVRGATVYPAEVEQALRTVDGVRAASVTNLP-G
Mvan_1980|M.vanbaalenii_PYR-1 LDADGFLYYHGRCDDMFKVRGATVYPSEVEQALRSLDGVSAAFVTNVA-G
MMAR_4027|M.marinum_M LDANGYLFYHGRSDDMFKVSGATVYPVEVETALRTIDGVSNAFVTNVA-G
MAV_1577|M.avium_104 LDDAGFLFYHGRADDMFKVSGATVYPSEVERALRTIDGVDGAVVTNVP-G
TH_4352|M.thermoresistible__bu LDADGFLFYHGRRDDMFKVSGATVYPGEVADALRSIDGVRAAYVTDVADG
MSMEG_2215|M.smegmatis_MC2_155 LDSDGFLFYHGRSDDMFKVSGATVYPGEAQHALRGIAGVRAAYVVNVP-D
MLBr_02546|M.leprae_Br4923 FDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIGVDDK
.* . * .* .:* * .*:* * : * . . .:
Mb3591|M.bovis_AF2122/97 RLGEVGRAFVVARPGTGLDEASVIAYTREHLANFKTPRSVRFVD---VLP
Rv3561|M.tuberculosis_H37Rv RLGEVGRAFVVARPGTGLDEASVIAYTREHLANFKTPRSVRFVD---VLP
MUL_4126|M.ulcerans_Agy99 RLGEVGRAFIVARPGFNLDEKSVIDYTREHLANFKAPRSVRFVD---TLP
MAB_0596c|M.abscessus_ATCC_199 RLGEVGKAYIVLKPGATLNADSVIEYSAKHVANFKKPRFVDFLD---ELP
Mflv_4364|M.gilvum_PYR-GCK PEGNRVGAAVVCD--ASLTVDVLRRAAREVLSSFKVPSVWALLPDEAAIP
Mvan_1980|M.vanbaalenii_PYR-1 PEGDRVGAAVVCD--APATAERLRSAAREVLSSFKVPTVWLLLDSQDGVP
MMAR_4027|M.marinum_M AQGQRVAAAVVCST-PALTSEDLRTSAGQVLSAFKVPTVWLLLADDANIP
MAV_1577|M.avium_104 ATGDRVGAAVVCR---ELTAAQLRAAARNLLSSFKVPSVWLVLRSDDDLP
TH_4352|M.thermoresistible__bu RGGDRVGAAVVGT---VTDVGQLRREARKLLSSFKVPTVWLLLDSDDAVP
MSMEG_2215|M.smegmatis_MC2_155 HQRNRVGAVVVTD---GMSARDLCAAAREVLSPFKVPSVWALLDDEAAVP
MLBr_02546|M.leprae_Br4923 EWGARLRAFVVKKQDATIDEDAIKAYVRDHLARYKVPREVIFLE---ELP
* :* : . :: :* * .: :*
Mb3591|M.bovis_AF2122/97 RNAAGKVSKPQLRELG-------------
Rv3561|M.tuberculosis_H37Rv RNAAGKVSKPQLRELG-------------
MUL_4126|M.ulcerans_Agy99 RNAGGKVVKPQLRELA-------------
MAB_0596c|M.abscessus_ATCC_199 RNLGGKVVKPTLRAMHGEQRRAQKESS--
Mflv_4364|M.gilvum_PYR-GCK RGATGKVDTARLRELLQD-----------
Mvan_1980|M.vanbaalenii_PYR-1 RGATGKVDVTRLRALLEQS----------
MMAR_4027|M.marinum_M RGPTGKVDLQRLRDMLANTA---------
MAV_1577|M.avium_104 RGVTGKVDVRRLRELLADADRRQETRVQG
TH_4352|M.thermoresistible__bu RRATGKVDVNRLRDLLATHPDRVVT----
MSMEG_2215|M.smegmatis_MC2_155 IGATGKVDNNGLRAMLAERGER-------
MLBr_02546|M.leprae_Br4923 RNPTGKVLKRELRDFEVH-----------
*** ** :