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VSDKPFQTIRQVPVVIVGAGPTGITAATLLAQYGVESMVLERWTDVYPQPRAVHLDDEIYRVIARLGIAD EFAAISRPTQGLRLLDNRFNVLTEFHRDPARSLHGFPQANMFDQPELETLLRANLKRYAHAQLRGGAEVI EICDTNELLRVTFTDRSDGRVHHVDAEYVLGCDGANSMVRSHVGSTMADLNFQQRWLVVDVATECDLAQW DGVYQVCDPFRAATYMRIGQSRYRWEFQLRPGERTEDFNSISALRPLIAPWTTDIAEGDLTLLRVAEYTF RAQIADQWRRGNIFILGDAAHLTPPFIGQGMGAGLRDAMNLSWKIAAVRCGTLTADALDTYQQERKPHTR QLIRLALAIGQFMTGGGRLGNVVRRALLPRVRFVPGLRDKISDSTTPALHASALVHKTHRLCRSRQLAGT LCPNPLLPEGQRLDAVIGTRFAVITSTSLGEAQELLLRRRDVLVVVTPPASSLADWLRRGGVKAALVRPD RTVMRAARDTVALCSWADRALQAS
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_2987 | mhpA | - | 100% (514) | 3-(3-hydroxy-phenyl) propionate hydroxylase |
| M. marinum M | MMAR_1503 | - | 3e-59 | 29.62% (503) | FAD-dependent oxidoreductase |
| M. marinum M | MMAR_4777 | mhpA | 4e-52 | 32.73% (440) | hydroxylase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1780 | - | 8e-09 | 23.40% (312) | hypothetical protein Mb1780 |
| M. gilvum PYR-GCK | Mflv_2401 | mhpA | 5e-56 | 33.18% (440) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. tuberculosis H37Rv | Rv1260 | - | 3e-09 | 23.35% (334) | hypothetical protein Rv1260 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_2573c | mhpA | 1e-166 | 58.32% (487) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. avium 104 | MAV_1804 | mhpA | 1e-174 | 63.98% (472) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. smegmatis MC2 155 | MSMEG_4866 | mhpA | 1e-52 | 31.08% (518) | 3-(3-hydroxyphenyl)propionate hydroxylase |
| M. thermoresistible (build 8) | TH_0968 | - | 8e-52 | 33.71% (445) | 3-(3-hydroxy-phenyl)propionate hydroxylase |
| M. ulcerans Agy99 | MUL_4482 | - | 1e-10 | 25.00% (336) | hypothetical protein MUL_4482 |
| M. vanbaalenii PYR-1 | Mvan_4906 | mhpA | 1e-180 | 62.15% (494) | 3-(3-hydroxyphenyl)propionate hydroxylase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_2987|M.marinum_M -----------------------------------------MSDKPFQTI
MAV_1804|M.avium_104 --------------------------------------------------
Mvan_4906|M.vanbaalenii_PYR-1 --------------------------------------------------
MAB_2573c|M.abscessus_ATCC_199 --------------------------------------------MNASAQ
Mflv_2401|M.gilvum_PYR-GCK ----------------------------------------------MTEQ
TH_0968|M.thermoresistible__bu ----------------------------------------------MTER
MSMEG_4866|M.smegmatis_MC2_155 ---------------------------------------------MMNSP
Rv1260|M.tuberculosis_H37Rv --------------------------------------------------
MUL_4482|M.ulcerans_Agy99 ------------------------------------------------MV
Mb1780|M.bovis_AF2122/97 MIATMPSMARRSRHDNKITTPAVDCLTIERLDSPASGAPQVTPYARALMG
MMAR_2987|M.marinum_M RQVPVVIVGAGPTGITAATLLAQYGVESMVLERWTDVYPQPRAVHLDDEI
MAV_1804|M.avium_104 --MPVVIVGAGPTGITAATLLAQHGIRCLVLERWDDVYPQPRAVHLDDEV
Mvan_4906|M.vanbaalenii_PYR-1 --MPVVVVGGGPTGVTAATLLAQYGVDTLVLDRWSQVYPQPRAVHLDDEI
MAB_2573c|M.abscessus_ATCC_199 LSFPVVIIGAGPTGVTAATLLAQHGVQCLLLERWATVYPQPRAVHLDDEI
Mflv_2401|M.gilvum_PYR-GCK TDVDVVIVGAGPAGLTLANILGLEGVRVLVVDERDKLIDYPRGVGLDDES
TH_0968|M.thermoresistible__bu --VDVVIVGAGPSGLTLANILGLQGVRTLVVEERDTLIDYPRGVGLDDEA
MSMEG_4866|M.smegmatis_MC2_155 GAVDVVIVGAGPVGLTLANILGGQGVRTLIIEERETLIDYPRGVGLDDES
Rv1260|M.tuberculosis_H37Rv -MKTVVVSGASVAGTAAAYWLGRHGYSVTMVERHPGLRPGGQAIDVRGPA
MUL_4482|M.ulcerans_Agy99 GVQKVLISGASVAGLTTAYWLEQQGYSVTIVERHPGLRPGGQAIDVRGPA
Mb1780|M.bovis_AF2122/97 ETTTCAIIGGGPAGMVLGLLLARAGVQVTLLEKHGDFLRDFRGDTVHPTT
: *.. * . . * * :::. . :. :
MMAR_2987|M.marinum_M YRVIARLGIADEFAAISRPTQGLRLLDNRFNVLTEFHRDPARSLHGFPQA
MAV_1804|M.avium_104 RRILARLGINDEFAAISRPALGLRLLDPEFGVLAQFNRDPAESVHGFPQA
Mvan_4906|M.vanbaalenii_PYR-1 CRILARLGVAEEFARISRPARGLRLVDRNLAVLAEFRRDTAVTRSGYPAA
MAB_2573c|M.abscessus_ATCC_199 YRILAQLGIAEQFATISRPGAGMRLLDHRGQILSQIARAPIG-THGYPQA
Mflv_2401|M.gilvum_PYR-GCK LRTFQAIGLVDRVLPHTVPNQILRFFDAKRNLLAEMAPPDAR--FGWPKR
TH_0968|M.thermoresistible__bu LRTFQSIGLVDRILPHTVPNQILRFFDAKRRLLAEMAPPDAR--FGWPKR
MSMEG_4866|M.smegmatis_MC2_155 LRTFQSIGLVDAILPHTVPNQILRFYDANRRLLAEMAPPDAR--FGWPKR
Rv1260|M.tuberculosis_H37Rv LDVLERMGLLAAAQEHKTRIRGASFVDRDGNELFRDTESTPTGGPVNSPD
MUL_4482|M.ulcerans_Agy99 LTVLDRMGILDAARDRKTGIRGASVVDRDGNELSRDTESTPTGGPIDSPN
Mb1780|M.bovis_AF2122/97 MRLLDELGLWERFAALPYSEVRTATLHSNGRAVTYIDFERLH--QPYPYV
: :*: . : .
MMAR_2987|M.marinum_M NMFDQPELETLLRANLKRYAHAQLRGGAEVIEICDTN-ELLRVTFTDRSD
MAV_1804|M.avium_104 NMFDQPEFEALLRRNLKNFPGAVMRGNSEVTDVTQTEVGRVRVTFADRAT
Mvan_4906|M.vanbaalenii_PYR-1 NMFDQPELENLLRRNLTRFRRARFRGDVEVLDIAVDAAGVTRVAFSDRLS
MAB_2573c|M.abscessus_ATCC_199 NMFDQPELEQLLRANLTNYPQVSLRGGVEVTTIAHISASQVQVTFTDRDS
Mflv_2401|M.gilvum_PYR-GCK NGFVQPMVDAELFGGLQRFDNVEVRFGHRMHQCTQTTDR----VEVEFDG
TH_0968|M.thermoresistible__bu NGFVQPMVDAELYAGLDRFDHVEVRFGHRMQNCVEASDAGEDGVTVEFAD
MSMEG_4866|M.smegmatis_MC2_155 NGFVQPMVDAELLAGLDRFDHVEVMWGRRMQTIAEDADG----VTVEVSG
Rv1260|M.tuberculosis_H37Rv IELLRDDLVELLYG--ATQPSVEYLFDDSISTLQDDGDS----VRVTFER
MUL_4482|M.ulcerans_Agy99 IELLRDDLIELLDG--TIQSTTEFIFDDSIATLQDDGAA----VEVTFVR
Mb1780|M.bovis_AF2122/97 AMVPQWDLLNLLAEAAQAEPSFTLRMKTEVTGLLREGGKVTGVRYQGAEG
. : . * :
MMAR_2987|M.marinum_M -GRVHHVDAEYVLGCDGANS-MVRSHVGSTMADLNFQQRWLVVDVATECD
MAV_1804|M.avium_104 -GEGHVVDADYVLGCDGANS-VVRSAIGAAMDDLHFEQRWLVVDVATDVD
Mvan_4906|M.vanbaalenii_PYR-1 -GRRSVITADYVLGCDGANS-MVRNAIGATMRNLKFDQRWLVVDVATTAD
MAB_2573c|M.abscessus_ATCC_199 -GDEHKVTATYVLGCDGANS-VVRAAIGASMTARRFAQRWLVVDLNTDAD
Mflv_2401|M.gilvum_PYR-GCK --GQASVSARYVVGCDGGRS-VTRRLMGVSFDGTTSSTRWLVVDIANDPL
TH_0968|M.thermoresistible__bu --GHPPVRASYVVGCDGGRS-TTRRLMGVSFDGTTSSTRWLVVDVANDPL
MSMEG_4866|M.smegmatis_MC2_155 PDGPASVHAQYVVGCDGGRS-ATRHLMGVSFDGTTSSTRWVVIDLANDPL
Rv1260|M.tuberculosis_H37Rv ---AAAREFDLVIGADGLHSNVRRLVFGPEEQFVKRLGTHAAIFTVPNFL
MUL_4482|M.ulcerans_Agy99 ---SGTRTFDFLIGADGLHSNVRRKVFGPEEQFIKRLGTYAAIFTVPNFL
Mb1780|M.bovis_AF2122/97 ---PGELRAELTVACDGRWS-IARHEAG--LKAREFPVNFDVWWFKLPRE
:..** * * * .
MMAR_2987|M.marinum_M LAQWDGVYQVCDPFRAATYMRIGQSRYRWEFQLRPGERTEDFNSISALRP
MAV_1804|M.avium_104 LNQWDGVYQVCSPVRAATYMRIGATRYRWEFRLLPGETADDYRSCAALRS
Mvan_4906|M.vanbaalenii_PYR-1 LQQWEGVHQICDPRRAGTYMRIGDTRYRWEFALSAGESADDFATLSALLP
MAB_2573c|M.abscessus_ATCC_199 LGHWDGVHHLCDPDRATTFMRVGERRYRWEFKLHPGETAADYDTVEKLRP
Mflv_2401|M.gilvum_PYR-GCK GHPNSEVG--ADPRRPYVSIAIAHGIRRFEFMIHPDETDEEADDPAFVRR
TH_0968|M.thermoresistible__bu GHPNSEVG--ADPARPYVSISIAHGIRRFEFMIHPHETDAEVDDPAFVRR
MSMEG_4866|M.smegmatis_MC2_155 GHPNSEVG--ADPQRPYASISIAHGIRRFEFMIHADETDEQAEDPEFVAE
Rv1260|M.tuberculosis_H37Rv ELDYWQTWHYGDSTMAGVYSARNNTEARAALAFMDTELRIDYRDTEAQFA
MUL_4482|M.ulcerans_Agy99 ELDYWQKWHYGDNTMAGVYSARDNSEARAALGFMDTELQIDYRDTEAQFA
Mb1780|M.bovis_AF2122/97 GDAEFSFLPRFSPGKGLGVIPREGYFQIAYLGPKGTDAQLRERGIEEFRR
. : :
MMAR_2987|M.marinum_M LIAPWTTDIAEGDLTLLRVAEYT-------FRAQIADQWRRGNIFILGDA
MAV_1804|M.avium_104 LISPWVGDVADEDLELVRVAEYT-------FRAQLANRWRDRNVFLLGDA
Mvan_4906|M.vanbaalenii_PYR-1 LLAPWTRHVAEADLQLVRVAEYT-------FRAQIADHWRSGSIFLLGDA
MAB_2573c|M.abscessus_ATCC_199 LIAVWTGASAISELTLIRVTEYT-------FRAHVASHWRDRNVFILGDA
Mflv_2401|M.gilvum_PYR-GCK MLGQLIPYPER--VDMIRHRVYT-------HHSRIAGSFREGRLMLAGDA
TH_0968|M.thermoresistible__bu MLGRRIPYPER--VDMIRYRVYT-------HHSRIAGSFRKGRLLLAGDA
MSMEG_4866|M.smegmatis_MC2_155 LLRPFVPHPDR--VDVIRRRVYT-------HHSRIAGSFRKGRMLLAGDA
Rv1260|M.tuberculosis_H37Rv ELQRRMAEDGWVRAQLLHYMRSAPDFYFDEMSQILMDRWSRGRVALVGDA
MUL_4482|M.ulcerans_Agy99 ELERRMIEDGWVRAQLLHYMRSAPDFYFDEMAQIVMDSWAKGRVALVGDA
Mb1780|M.bovis_AF2122/97 DVSELLPEATASVAALASTDEVKHLN----VKVNRLRRWHIDGLLCIGDA
: : : : ***
MMAR_2987|M.marinum_M AHLTPPFIGQGMGAGLRDAMNLSWKIAAVRCGTLTADALDTYQQERKPHT
MAV_1804|M.avium_104 AHLTPPFIGQGMGAGLRDAMNLAWKLAGVLGEDLPEEVLETYEQERKPHA
Mvan_4906|M.vanbaalenii_PYR-1 AHLTPPFIGQGMGAGLRDAMNLAWKLAGVHHGSLPPTVLDSYEQERKPHA
MAB_2573c|M.abscessus_ATCC_199 AHLTPPFVGQGMGAGIRDAANLTWKLAGVLDGQLPTRVLDTYEQERKPHA
Mflv_2401|M.gilvum_PYR-GCK AHLMPVWQGQGYNSGIRDAANLGWKLAAVVTGRAGEELLDTYDVERRKHA
TH_0968|M.thermoresistible__bu AHLMPVWQGQGYNSGIRDAANLGWKLAAVVNGHAHDALLDTYDLERREHA
MSMEG_4866|M.smegmatis_MC2_155 AHLMPVWQGQGYNSGIRDAFNLGWKLAAVVRGQAGDALLDTYDAERRKHA
Rv1260|M.tuberculosis_H37Rv GYCCSPLSGQGTSVALLGAYILAGELKAAG---------DDYQLGFANYH
MUL_4482|M.ulcerans_Agy99 AYCCSPLSGQGTSVAVLGAYILAGELAAAG---------NDYQRGFANYH
Mb1780|M.bovis_AF2122/97 AHAMSPVAGVGINLAVQDAVAAATILAEPLR----EHRVSSRHLAAVRRR
.: . * * . .: .* : . .
MMAR_2987|M.marinum_M RQLIRLALAIGQFMTGGGRLGNVVRRALLPRVRFVPGLRDKISDS---TT
MAV_1804|M.avium_104 RALIALALGMGWAMTAGGGIGDAIRRTVVPRLHLVPGMRGRIVNS---RT
Mvan_4906|M.vanbaalenii_PYR-1 RHMISLALNMGRAMTAGGRLGAAVRRLIVPRLHLIPGMRGKVIDS---ET
MAB_2573c|M.abscessus_ATCC_199 WKMITIALAVGAAMTARGTAATAIRNLLVPRLQYIPGLRSRIQHS---GT
Mflv_2401|M.gilvum_PYR-GCK RAMIDLSTMVGRVISPTNRKVAALRDRVIHAASAVPSLKRYVLEMRFKPM
TH_0968|M.thermoresistible__bu RAMIDLSTMVGRVISPTNRRVAALRDRLIHAASVVPTLKRYVLEMRFKPM
MSMEG_4866|M.smegmatis_MC2_155 RAMIDLSTMVGRVISPTNRKVAALRDKLIRGASIVPTLKRYVLEMRFKPM
Rv1260|M.tuberculosis_H37Rv AEFHGFVERNQWLVSDNIPGGAPIPQEEFERIVHSITIKDY---------
MUL_4482|M.ulcerans_Agy99 AEFSGFGERNQWLVTDNIPGGEPIPEEAFERIVNSLELEDYRQG------
Mb1780|M.bovis_AF2122/97 RAFPTAVTQAVQRVLHRRLLGPLLQGRDPTPPAALLGLVERLPWLS--AV
: : : :
MMAR_2987|M.marinum_M PALHASALVHKTHRLCRSRQLAGTLCPNPLLPEGQR----LDAVIGTRFA
MAV_1804|M.avium_104 PALRRSALVVKSR---LRRDLAGTLCPNPLLDNGAR----FDNVVGNRFA
Mvan_4906|M.vanbaalenii_PYR-1 PALKASSLVRRTP---ASRRVAGRLCPNPVLADGRR----LDEALGSGFG
MAB_2573c|M.abscessus_ATCC_199 PALTRSALVNKTP---RCRQLAGTQIPNLVLSQGHR----IDTLLGSGFA
Mflv_2401|M.gilvum_PYR-GCK PRYQQGAVLHHAHA--APNSPTGTLFIQPRVDTRDTQNALLDDVLGTGFA
TH_0968|M.thermoresistible__bu PRYLQGAVYHGGPR--SKSSPTGTLFIQPRVDTRDAENVLLDDVLGPGFA
MSMEG_4866|M.smegmatis_MC2_155 PRYHEGAVYHAKPP--TPASPVGTLFIQPRVDTREQDNVLLDDVLGTGFA
Rv1260|M.tuberculosis_H37Rv --------------------------------------------------
MUL_4482|M.ulcerans_Agy99 --------------------------------------------------
Mb1780|M.bovis_AF2122/97 PAYFVGVGVRPEHAPAFARRGPGNRKGP----------------------
MMAR_2987|M.marinum_M VITS-----TSLGEAQELLLRRRDVLVVVTPPASSLADWLRRGGVKAALV
MAV_1804|M.avium_104 LIAS-----LPLTKGQRIELDHRGAVVLSVKPGCELDRWLRRGRVTSRGV
Mvan_4906|M.vanbaalenii_PYR-1 VVTT-----GALTPAQQALVERCGARALCVAPATELASWLRRGRVTTAMI
MAB_2573c|M.abscessus_ATCC_199 LVAS-----ETVDADQQAQLRRRGAAIIVEPRGSELDRWLRRGGARAALI
Mflv_2401|M.gilvum_PYR-GCK VVCWSNNLRAVLGEEAFGRWKALGAKFVEVRPMSQLR-WPGHDDPDVAVI
TH_0968|M.thermoresistible__bu VLCWSNNLRAVLGEDAFSHWKSLGATFVEARPITQLH-WPGHDDPDVVVV
MSMEG_4866|M.smegmatis_MC2_155 VLCWNNNPRTLLGEAAFTRWKALGATFIAARPSTQLH-WTKDDDPDVVIV
Rv1260|M.tuberculosis_H37Rv --------------------------------------------------
MUL_4482|M.ulcerans_Agy99 --------------------------------------------------
Mb1780|M.bovis_AF2122/97 --------------------------------------------------
MMAR_2987|M.marinum_M RPDRTVMRAARDTVALC----------SWADRALQAS-------------
MAV_1804|M.avium_104 V-------------------------------------------------
Mvan_4906|M.vanbaalenii_PYR-1 RPDRTVMSAGRDVDALCRQFLSGNLVGVWNSVAATPD-------------
MAB_2573c|M.abscessus_ATCC_199 RPDRTVMQTARSPSQIAFTTTLFAPTHQPAPTYRETP-------------
Mflv_2401|M.gilvum_PYR-GCK GDRTGALKAWFDVHTESVLFVRPDRCIAAACIAQRAPEISEGLFAALHLT
TH_0968|M.thermoresistible__bu GDRTGALKGWFDVHTDSVLFLRPDRCIAAACIAQRAPETSAALSEVMCLT
MSMEG_4866|M.smegmatis_MC2_155 GDRTGALKAFFDAHTESVLVLRPDRCIAGADIAQRAPELSTALFGILHLR
Rv1260|M.tuberculosis_H37Rv --------------------------------------------------
MUL_4482|M.ulcerans_Agy99 --------------------------------------------------
Mb1780|M.bovis_AF2122/97 --------------------------------------------------
MMAR_2987|M.marinum_M --------------------------------------
MAV_1804|M.avium_104 --------------------------------------
Mvan_4906|M.vanbaalenii_PYR-1 --------------------------------------
MAB_2573c|M.abscessus_ATCC_199 --------------------------------------
Mflv_2401|M.gilvum_PYR-GCK SGAHLTQGGGTDGEKPDRAVLHVAQPAAESSGTGAGTS
TH_0968|M.thermoresistible__bu ------QGGGNGAASP---VLHVAQSAAEPSGTATRST
MSMEG_4866|M.smegmatis_MC2_155 ------QGGENGATGP---VLYVPQPTAESSGTVGRAS
Rv1260|M.tuberculosis_H37Rv --------------------------------------
MUL_4482|M.ulcerans_Agy99 --------------------------------------
Mb1780|M.bovis_AF2122/97 --------------------------------------