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THRASALISAQEWFSAGERVGYDAERPGINPRSPLRAFIRRAAGTGVTRTFLPGWPDGSYGWAKVEAFLS SRFHFPRIYLDYIGHGDSDKPRDYPYSTFERADLVEALWHAEGIAQTVVVAFDYSCIVSLELLARRIDRE RAGNDQRTRITACLLANGGIFADGHTHAWYTTPLLTSPLGAAITPIGQRSWRMFAPFLRPVFSRGYPLSA AEMKELHDAISRRDGVRVLPATAGFVDEHREHAARWDLARIISALGDEVAFGVVGSAEDPFEGEQLRLAR ERLADSVEITELAGGHLTTAEQPDRLAEVIAALPERS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. tuberculosis H37Rv | Rv3176c | mesT | - | 100% (318) | PROBABLE EPOXIDE HYDROLASE MEST (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE) |
| M. tuberculosis H37Rv | Rv1834 | - | 9e-21 | 28.01% (307) | hydrolase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3202c | mesTa | 1e-119 | 100.00% (201) | epoxide hydrolase MesTa |
| M. gilvum PYR-GCK | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4300 | - | 7e-15 | 27.20% (250) | putative hydrolase, alpha/beta fold |
| M. marinum M | MMAR_2711 | - | 4e-18 | 27.04% (270) | hydrolase |
| M. avium 104 | - | - | - | - | - |
| M. smegmatis MC2 155 | MSMEG_2777 | - | 2e-18 | 27.99% (268) | alpha/beta hydrolase fold |
| M. thermoresistible (build 8) | TH_4027 | - | 1e-21 | 29.14% (278) | PUTATIVE hydrolase |
| M. ulcerans Agy99 | MUL_3053 | - | 9e-19 | 27.04% (270) | hydrolase |
| M. vanbaalenii PYR-1 | - | - | - | - | - |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_2711|M.marinum_M ----------------------MTSPSVSDWRAGGGWINTAA--------
MUL_3053|M.ulcerans_Agy99 MNCRPITLVTLLSWSSRWYARRVTSPSVSDWRAGGGWINTAA--------
TH_4027|M.thermoresistible__bu ---------------------------VARWRAEGRWIRTGV--------
MAB_4300|M.abscessus_ATCC_1997 -----------------------MDAELEEWKAAGHYFDYLG--------
MSMEG_2777|M.smegmatis_MC2_155 -----------------------MHDELQRWKDAGQYFDYLG--------
Rv3176c|M.tuberculosis_H37Rv -----------------MTHRASALISAQEWFSAGERVGYDAERPGINPR
Mb3202c|M.bovis_AF2122/97 -----------------MTHRASALISAQEWFSAGERVGYDAERPGINPR
* * .
MMAR_2711|M.marinum_M --GKVFVRSG---EGIAPTVLLLHGFPSSSFDFRSVIPYLAGQA---WLT
MUL_3053|M.ulcerans_Agy99 --GKVFVRSG---EGIAPTVLLLHGFPSSSFNFRSVIPYLAGQA---WLT
TH_4027|M.thermoresistible__bu --GSVFVRSA---SGRGSPVVLLHGYPSSSYDYRAVLPELGDRG---WVT
MAB_4300|M.abscessus_ATCC_1997 --FDIFYRVE----GSGPPLLLIHGYPFNSWDWALIWPTLVERFT--VIA
MSMEG_2777|M.smegmatis_MC2_155 --FDIFYRVDGVAPGTGPNLLLIHGYPFNSWDWSLIWPTLTQRFT--VIA
Rv3176c|M.tuberculosis_H37Rv SPLRAFIRRA---AGTGVTRTFLPGWPDGSYGWAKVEAFLSSRFHFPRIY
Mb3202c|M.bovis_AF2122/97 SPLRAFIRRA---AGTGVTRTFLPGWPDGSYGWAKVEAFLSSRFHFPRIY
* * * . :: *:* .*:.: : . * : :
MMAR_2711|M.marinum_M MDFLGFGLSDKPRPHRYSLLEQADLVQNVVAQNVTGPVVVLAHDMGTSVT
MUL_3053|M.ulcerans_Agy99 MDFLGFGLSDKPRPHRYSLLEQADLVQNVVAQNVTGPVVVLAHDMGTSVT
TH_4027|M.thermoresistible__bu FDFLGFGLSDKPRPHRYSLFEQADLVQTVVRSVTDEPVLLIAHDMGTSVA
MAB_4300|M.abscessus_ATCC_1997 PDMIGMGFSDKPVQYGYSVLDHADMHEALLSYLGIERFHLLTHDLGNSVG
MSMEG_2777|M.smegmatis_MC2_155 PDMIGMGFSDKPVAYEYRVTDHADIHEALLAHLGITSTHILAHDLGDSVG
Rv3176c|M.tuberculosis_H37Rv LDYIGHGDSDKPRDYPYSTFERADLVEALWHAEGIAQTVVVAFDYSCIVS
Mb3202c|M.bovis_AF2122/97 LDYIGHGDSDKPRDYPYSTFERADLVEALWHAEGIAQTVVVAFDYSCIVS
* :* * **** : * ::**: : : :::.* . *
MMAR_2711|M.marinum_M TELLARDLEGRLP-----FELQRAVLSNGSVILERASLRPIQKVLR-GPL
MUL_3053|M.ulcerans_Agy99 TELLARDLEGRLP-----FELQRVVLSNGSVILERASLRLIQKVLR-GPL
TH_4027|M.thermoresistible__bu TELMARDLQGQLP-----FRLQRVVLSNGSVLLDRASLRPIQKVLR-GRF
MAB_4300|M.abscessus_ATCC_1997 QEMLARFEFEQQSRG--RVPIDSVTWLNGGLFIEAYRPRIAQTLMSRTPL
MSMEG_2777|M.smegmatis_MC2_155 QEMLARHEFGGQAYG--GFQIESVTWLNGGLFNEAYRPRLMQKVMSRTPL
Rv3176c|M.tuberculosis_H37Rv LELLARRIDRERAGNDQRTRITACLLANGGIFADGHTHAWYTTPLLTSPL
Mb3202c|M.bovis_AF2122/97 LELLARRIDRERAGNDQRTRITACLLANGGIFADGHTHAWYTTPLLTSPL
*::** . : **.:: : . : :
MMAR_2711|M.marinum_M GPIAARLVN----RGGFTRGFGKLFSAQHPLSAQEAEAQWELLTHNNGHR
MUL_3053|M.ulcerans_Agy99 GPIAARLVN----RGGFTRGFGKLFSAQHPLSAQEPEAQWELLTHNNGHR
TH_4027|M.thermoresistible__bu GPVVSQLVN----EPMFIRGLGKVFSKQRPLSAAEGAAQWALMSYHDGHR
MAB_4300|M.abscessus_ATCC_1997 GDLVSRFQGTPVTRRIMDAAVSEMFGPDTKPSPRLLSLFQQVLEYNDGAR
MSMEG_2777|M.smegmatis_MC2_155 GDILSPLQGSPLSRRVFESTLDQMFGVNTKPSREMIEKFHQILDHNDGKR
Rv3176c|M.tuberculosis_H37Rv GAAITPIGQR--SWRMFAPFLRPVFSRGYPLSAAEMKELHDAISRRDGVR
Mb3202c|M.bovis_AF2122/97 GAAITPIGQR--SWRMFAPFLRPS-SR-----------------------
* : : : . .
MMAR_2711|M.marinum_M IAHLLISYLEERERYAQRWHN----AVRDWPKPLGFVWGLDDPVATTNVL
MUL_3053|M.ulcerans_Agy99 IAHLLISYLEERERYAQRWNN----AVRDWPKPLGFVWGLDDPVATTNVL
TH_4027|M.thermoresistible__bu IMHLLTAYLNERVRYADRWHG----AVADWPKPLGLLWGTDDPVATTDVL
MAB_4300|M.abscessus_ATCC_1997 VTHQVGRFINDRYDNRSRWVR----AMRETAVPMRMIDGPIDPNSGLHMA
MSMEG_2777|M.smegmatis_MC2_155 VLHKVGRFVTDRYTHRNRWVR----AMRETSVPMRLIDGPSDPNSGRHMA
Rv3176c|M.tuberculosis_H37Rv VLPATAGFVDEHREHAARWDLARIISALGDEVAFGVVGSAEDPFEGEQLR
Mb3202c|M.bovis_AF2122/97 --------ADTH--------------------------------------
:
MMAR_2711|M.marinum_M NGLRELRPNAGVIELPG-LGHYPQVEDPKAYTQAALDLL-----VG
MUL_3053|M.ulcerans_Agy99 NGLRELRPNAGVIELPG-LGHYPQVEDPKAYTQPALDLL-----VG
TH_4027|M.thermoresistible__bu AGLRELRSDAEVIELAG-VGHYPQIEVPEVFARAALELLGLNRTIG
MAB_4300|M.abscessus_ATCC_1997 ERYLEVIHDPDVVLLDDNIGHWPQIEAPEAVLTHFLEHIDRITP--
MSMEG_2777|M.smegmatis_MC2_155 QRYLEVIPDPDVVMLADDIAHWPQIEAPDAVLAHFLEHIDRVVAL-
Rv3176c|M.tuberculosis_H37Rv LARERLADSVEITELAG--GHLTTAEQPDRLAEVIAALPERS----
Mb3202c|M.bovis_AF2122/97 ----------------------------------------------