For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
VRTVVLATCVFLAALTGCSPGDRVDLGAGAADALIAAIAGEPDQLDPHKTTSYFSFEVLENVFDTLVEPD GNLQMQPALARAWEVSPDQLTWTFHLRPGVTFHDGTPLTADDVVFSYRRIIDEQLANSDKFSSVAAVDAP DPNTVVIRVDRPTPNMLTNLGGFKGMAIVSRANVESGRIATQPVGTGPFRFLGQKSGDSISLAANPNYWD GPPGVSGVTYRFISEPSTALSALQAGEIDWTDSVPPQRVTQLRDDESLELAVTPSNDYWYLALNEAREPW NDVRVRQAVAYAVDRDAIVQATSYGTAAVNQLAIPQGNPWFTPYDRYRDDGEQGLETARTLLREAGAQPR ELDMLVTTEYPETVTAAQIIADNLAPLGISVKIRTVDFATWLDEQNSGNFDMLMMGWLGNIDPDDFYYAQ HHTDGTSNAQKFSDPEVDRLLDASRVETDEAARKEQYAGAATLIADKVSYIYLYNPSVIQAWNPALSGYE ARRDGAVRFRSATFGADGGA
Operon Prediction Model: Genebank
Paralogs
species | id | gene | e-value | identity (len) | annotation |
M. vanbaalenii PYR-1 | Mvan_3773 | - | - | 100% (510) | extracellular solute-binding protein |
M. vanbaalenii PYR-1 | Mvan_1024 | - | 4e-36 | 27.52% (476) | extracellular solute-binding protein |
M. vanbaalenii PYR-1 | Mvan_0438 | - | 9e-31 | 24.15% (501) | extracellular solute-binding protein |
Closest Orthologs (e-value cutoff: 1e-4)
species | id | gene | e-value | identity (len) | annotation |
M. bovis AF2122 / 97 | Mb1311c | oppA | 3e-20 | 23.45% (563) | periplasmic oligopeptide-binding lipoprotein OppA |
M. gilvum PYR-GCK | Mflv_2761 | - | 0.0 | 86.61% (508) | extracellular solute-binding protein |
M. tuberculosis H37Rv | Rv1280c | oppA | 3e-20 | 23.45% (563) | periplasmic oligopeptide-binding lipoprotein OppA |
M. leprae Br4923 | MLBr_01121 | - | 6e-17 | 22.94% (449) | putative extracellular solute-binding dependent transport |
M. abscessus ATCC 19977 | MAB_0426 | - | 7e-18 | 25.89% (336) | peptide ABC transporter DppA |
M. marinum M | MMAR_4139 | oppA | 2e-17 | 22.88% (472) | periplasmic oligopeptide-binding lipoprotein OppA |
M. avium 104 | MAV_1429 | - | 2e-16 | 22.97% (492) | extracellular solute-binding protein, family protein 5 |
M. smegmatis MC2 155 | MSMEG_4354 | - | 0.0 | 79.68% (502) | dipeptide-binding protein of ABC transport system |
M. thermoresistible (build 8) | TH_4152 | - | 0.0 | 73.16% (503) | PUTATIVE extracellular solute-binding protein, family 5 |
M. ulcerans Agy99 | MUL_4002 | oppA | 1e-16 | 22.57% (474) | periplasmic oligopeptide-binding lipoprotein OppA |
CLUSTAL 2.0.9 multiple sequence alignment Mvan_3773|M.vanbaalenii_PYR-1 ----------------------------------MRTVVLATCVFLAALT Mflv_2761|M.gilvum_PYR-GCK -----------------------------MARTAVGAAVLAVCALVLSLT MSMEG_4354|M.smegmatis_MC2_155 ---------------------------------MAGMATAWLAALVLVFT TH_4152|M.thermoresistible__bu -------------------------------------VSNLVAVLLIAAT Mb1311c|M.bovis_AF2122/97 MADRGQRRGCAPGIASALRASFQGKSRPWTQTRYWAFALLTPLVVAMVLT Rv1280c|M.tuberculosis_H37Rv MADRGQRRGCAPGIASALRASFQGKSRPWTQTRYWAFALLTPLVVAMVLT MMAR_4139|M.marinum_M MLTNADR---HDGVQPGPGARSRRPTRPWAR-RRIRCAGAALLTVTLLLA MUL_4002|M.ulcerans_Agy99 -----------------------------------------MLTVTLLLA MAV_1429|M.avium_104 ------------------------------MGRCGFRPAALVAAVVLAVA MLBr_01121|M.leprae_Br4923 -------------------------------MIMLSRIVAALLVVGLILT MAB_0426|M.abscessus_ATCC_1997 -----------------------------------MRLRRLAIVLLVGIV .. . Mvan_3773|M.vanbaalenii_PYR-1 GCSPG-DRVDLGAGAADALIAAIAGEPDQLDPHKTT----SYFSFEVLEN Mflv_2761|M.gilvum_PYR-GCK GCNTG-ERVDLGDGAGGALIAAIAGEPDQLDPHKTT----AYFSFEVLEN MSMEG_4354|M.smegmatis_MC2_155 ACSTG-ERVDLGDEASGNLIAAIAGEPDQLDPQKTS----AYFSFEVLEN TH_4152|M.thermoresistible__bu GCAPG-QRVDLGD-LSGNLIAAIAGEPDQLDPHKTS----AYFSFQVLEN Mb1311c|M.bovis_AF2122/97 GCSASGTQLELAPTADRRAAVGTTSDINQQDPATLQDGGNLRLSLTDFPP Rv1280c|M.tuberculosis_H37Rv GCSASGTQLELAPTADRRAAVGTTSDINQQDPATLQDGGNLRLSLTDFPP MMAR_4139|M.marinum_M GCSAS-TQLPAATSGS--TAVGSSSDINPQDPATLEDGGDLRLSLTDFPP MUL_4002|M.ulcerans_Agy99 GCSAS-TQLPAATSGS--TAVGSSSDINPQDPATLEDGGDLRLSLTDFPP MAV_1429|M.avium_104 GCSPA---INLVPETGQNARIGTTSDINPRDPATLQDGGNLRLALTDFPP MLBr_01121|M.leprae_Br4923 GCSAS---NRLVPSAASNAALGKTSDINPQDPATLKDGGNLRLALTDFPA MAB_0426|M.abscessus_ATCC_1997 AVLPAGCGDLVTPAPPGYFSMYITEPEHTLVPGNTT----ENQGGRILRS . .. : . * . . : Mvan_3773|M.vanbaalenii_PYR-1 VFDTLVE----PDGNLQMQPALARAWEVSPD------------------- Mflv_2761|M.gilvum_PYR-GCK VFDTLVE----PDQDLQMKPALAESWEVSPD------------------- MSMEG_4354|M.smegmatis_MC2_155 VFDTLVE----PDENLQMQPALAESWEVSPD------------------- TH_4152|M.thermoresistible__bu VFDTLVE----PDEDLRMRPALAESWQVSAD------------------- Mb1311c|M.bovis_AF2122/97 NFNILHIDGNNAEVAAMMKATLPRAFIIGPDGSTTVDTNYFTSIELTRTA Rv1280c|M.tuberculosis_H37Rv NFNILHIDGNNAEVAAMMKATLPRAFIIGPDGSTTVDTNYFTSIELTRTA MMAR_4139|M.marinum_M NFNILHIDGNSAEVAAMVKATLPRAFIIGPDGSTTVDHDYFTSVELTKTN MUL_4002|M.ulcerans_Agy99 NFNILHIDGNSAEVAAMVKATLPRAFIIGPDGSTTVDHDYFTSVELTKTN MAV_1429|M.avium_104 NFNILHIDGNSADVSAMMKATLPRAFVIGPDGSTTVDTDYFTSVELTGTA MLBr_01121|M.leprae_Br4923 NFNILHIDGNNAEVTSMMKATMPRAFIIGTDGSTTVDTNYFTSIKLTSTS MAB_0426|M.abscessus_ATCC_1997 LFTALVEFN--NETAAVEYTGVAESITSHDN------------------- * * : . :..: : Mvan_3773|M.vanbaalenii_PYR-1 QLTWTFHLRPGVTFHDGTPLTADDVVFSYRR---IID-----EQLANSD- Mflv_2761|M.gilvum_PYR-GCK QLTWTFRLRPGVTFHDGTPLAAEDVVFSYRR---IID-----EQLANSD- MSMEG_4354|M.smegmatis_MC2_155 QLTWTFHLRDGVRWHDGTPLTADDVVYSYRR---IID-----EQLTNVD- TH_4152|M.thermoresistible__bu QRVWTFRLRPGVTFHDGSPLTADDVVFSYRR---IID-----EHLTNVD- Mb1311c|M.bovis_AF2122/97 PQVVTYTINPEAVWSDGTPITWRDIASQIHA---ISGADKAFEIASSSG- Rv1280c|M.tuberculosis_H37Rv PQVVTYTINPEAVWSDGTPITWRDIASQIHA---ISGADKAFEIASSSG- MMAR_4139|M.marinum_M PQVITYTINPKAFWSDGTPITWRDIASQIHA---TSGADNRFEIASSGG- MUL_4002|M.ulcerans_Agy99 PQVITYTINPKAFWSDGTPVTWRDIASQIHA---TSGADNRFEIASSGG- MAV_1429|M.avium_104 PQVVTYTINPKAVWSDGTPITWEDIASQIHA---LSGADKTFEIAGSSG- MLBr_01121|M.leprae_Br4923 PQVVTYTINPKAVWSDGTPITWRDIDRQIHA---NSGKNKAFEIAGTNG- MAB_0426|M.abscessus_ATCC_1997 -VNWTIKLKPGWTFHNGEPVTAQSYVDAWNYTALSTNAQGSSSFLANVDG * :. : :* *:: . . . . . . Mvan_3773|M.vanbaalenii_PYR-1 --------------KFSSVAAVDAPDPNTVVIRVDRPTPNMLTNLGG--- Mflv_2761|M.gilvum_PYR-GCK --------------KFSSVQAVEAPDPSTVVIRVDRPTPNMLTNLGG--- MSMEG_4354|M.smegmatis_MC2_155 --------------KFSAVTDVSAPDPATVRIVVSHPTPNLLTNLGG--- TH_4152|M.thermoresistible__bu --------------RLSAVTEVTAVDPLTVRITVARPTPNLLTNLGG--- Mb1311c|M.bovis_AF2122/97 --------------AERVASVTRGVDDRQAVVTFAKPYAEWRGMFAGNGM Rv1280c|M.tuberculosis_H37Rv --------------AERVASVTRGVDDRQAVVTFAKPYAEWRGMFAGNGM MMAR_4139|M.marinum_M --------------SDRVASVTPGVDDRQAIVTFAKPYAEWRGMFAGNGM MUL_4002|M.ulcerans_Agy99 --------------SDRVASVIPGVDDRQAMVTFAKPYAEWRGMFAGNGM MAV_1429|M.avium_104 --------------AERVASVTRGVDDRQAVVTFAKPYAEWRGMFAGNGM MLBr_01121|M.leprae_Br4923 --------------CERVASIRPGANDRQAIVTFAQPYAEWRGMFAGNGM MAB_0426|M.abscessus_ATCC_1997 FDDLQGDPAHKIAPRATQMRGLHVVDDVTFTVRLNTPFAIYPMTLGY--- : : . * . :. Mvan_3773|M.vanbaalenii_PYR-1 -FKGMAIVSRANVESGRIATQPVGTGPFRFLGQ-KSGDSISLAANPNYWD Mflv_2761|M.gilvum_PYR-GCK -FKGMAIVSRANVESGRIATHPVGTGPFSFLGQ-KSGDSISLRANPDYWA MSMEG_4354|M.smegmatis_MC2_155 -FKGMAIVQRGNVESGQIATHPIGTGPFAFAGR-KSGDSITLKANPHYWG TH_4152|M.thermoresistible__bu -FKGMAIVQRANVETGRIATHPVGTGPFEFRGA-RSGDSITLVANDDHWA Mb1311c|M.bovis_AF2122/97 LLPASMTATPEAFNKGQLDGPGPSAGPFVVSALDRTAQRIVLTRNPRWWG Rv1280c|M.tuberculosis_H37Rv LLPASMTATPEAFNKGQLDGPGPSAGPFVVSALDRTAQRIVLTRNPRWWG MMAR_4139|M.marinum_M LLPSSMTATPEAFNKGQLAGPGPSAGPFIVTSLDRSAQRIVLTRNPKWWG MUL_4002|M.ulcerans_Agy99 LLPSSMTATPEAFNKGQLTGPGPSAGPFIVTSLDRSAQRIVLIRNPKWWG MAV_1429|M.avium_104 LLPKSMTATPEAFNKGQLEGPGPSAGPFIVSSLDRTTQRIVLSRNPKWWG MLBr_01121|M.leprae_Br4923 LLPATMTATPEAFNKGQLDRPGPSAGPFIVSSLDRTTQRIVLTHNPKWWG MAB_0426|M.abscessus_ATCC_1997 -TAFLPLPKAFFRDPVKFGVHPIGNGPFKADGDWVRGVGFTLSRYDQYAG : :: . *** . : * Mvan_3773|M.vanbaalenii_PYR-1 -GPPGVSGVTYRFISEPSTALSALQAGEIDWTDSVPPQRVTQLRDDESLE Mflv_2761|M.gilvum_PYR-GCK -GPPGVAGVTFRFIPEPSTALSALQAGEVDWTDSVPPQRVSQLRDDESLQ MSMEG_4354|M.smegmatis_MC2_155 -PEPRVSGVTFRFISEPATALSALQAGEIDWTDSVPPQRVAQLRDDDSLT TH_4152|M.thermoresistible__bu -GPPRLNGVTFRFISEPATALSALQAGEIDWTDAIPTQRVTQLRNDDSLT Mb1311c|M.bovis_AF2122/97 -ARPRLDSITY-LVLDDAARLPALQNNTIDATGVGTLDQLTIAARTKGIS Rv1280c|M.tuberculosis_H37Rv -ARPRLDSITY-LVLDDAARLPALQNNTIDATGVGTLDQLTIAARTKGIS MMAR_4139|M.marinum_M -KKPRLDSITY-LVLDDAARIPALQNFTIDATGIGSLDQLTIAERTKGIS MUL_4002|M.ulcerans_Agy99 -KKPRLHSITY-LVLDDAARIPALQNFTIDATGIGSLDQLTIAERTKGIS MAV_1429|M.avium_104 -TRPRLDSITY-LVLDDAARLPALQNNTIDATGVGTLDQLTIAEHTAGIS MLBr_01121|M.leprae_Br4923 -TPPRLDSITY-LVLDDAARLPALQNNTIDATGVGTLDQLTTAQRTNGIS MAB_0426|M.abscessus_ATCC_1997 EKKAKAKGLIWRVYTEGLTAYTDMQAGALDIQLDLPDAAYENAKAEFGSA . .: : . : : . :* :* . . Mvan_3773|M.vanbaalenii_PYR-1 LAVTPSNDYWYLALNEAR-EPWNDVRVRQAVAYAVDRDAIVQATSYGTAA Mflv_2761|M.gilvum_PYR-GCK LTVTPSNDYWYLALNQAR-APWNDVRVRQAIAYAIDRDAIVQATSYGTAA MSMEG_4354|M.smegmatis_MC2_155 LAVTPSNDYWYLALNEAR-APWNDVRVRQAIAYGIDRDAIATATSYRTAA TH_4152|M.thermoresistible__bu LAVTPSNDYWYLALNHAR-APWHDLRVRRAIAFGIDREAIVTATGYGTAT Mb1311c|M.bovis_AF2122/97 IRRAPGPSWYHFTLNGAPGSILADKALRLAIAKGIDRYTIARVAQYG--L Rv1280c|M.tuberculosis_H37Rv IRRAPGPSWYHFTLNGAPGSILADKALRLAIAKGIDRYTIARVAQYG--L MMAR_4139|M.marinum_M IRRAPAPSWSHFTLNGAPGAVLADPALRLAVCKGIDRRTIAKVALYG--L MUL_4002|M.ulcerans_Agy99 IRRAPAPSWSHFTLNGAPGAVLADPALRLAVCKGIDRRTIAKVALYG--L MAV_1429|M.avium_104 IRRAPAPAWSHFTFNGAPGSILADRALRVAVSKGIDRQTIAKVVQYG--L MLBr_01121|M.leprae_Br4923 IRRTPAPRWSHFTFNGAVGSILADQGLRLAVSKGIDRRTIAKVVQYG--L MAB_0426|M.abscessus_ATCC_1997 YLERPRPDITSLGFPMYD-PRYRDVRVRQAISMAIDREAITQVIFSG--- * : : * :* *:. .:** :*. . Mvan_3773|M.vanbaalenii_PYR-1 VNQLAIPQGNPWFTPYDR--YRDDGEQGLETARTLLREAGAQ-------- Mflv_2761|M.gilvum_PYR-GCK ANQLAIPEGNPWFTPYDR--YREDGEEGLETARDLLRQANAT-------- MSMEG_4354|M.smegmatis_MC2_155 INQLAIPEGNPWFTSYDR--YRFD----LDESKRLLDEAGAH-------- TH_4152|M.thermoresistible__bu ANQLAIPPGDFWYTHYDR--YRYD----LDRARALLDEARAAGV------ Mb1311c|M.bovis_AF2122/97 TSDPVPLNNHVFVAGQDG--YQDNSGVVAYNPEQAKRELDALGWRRSGAF Rv1280c|M.tuberculosis_H37Rv TSDPVPLNNHVFVAGQDG--YQDNSGVVAYNPEQAKRELDALGWRRSGAF MMAR_4139|M.marinum_M TNDPAPLNNHVFVAGQEG--YQDNS--SPYDPEQAKRELDALGWKLNGKF MUL_4002|M.ulcerans_Agy99 TNDPAPLNNHVFVAGQEG--YQDNS--SPYDPEQAKRELDALGWKLNGKF MAV_1429|M.avium_104 TSNPVALGNHIYVAGQQG--YQDNSTTVPYDPAAAGRELDALGWKLHGRF MLBr_01121|M.leprae_Br4923 ANNPEPLNNHVYVDGQEG--YQDNSGVVGYDPEQAQHELDALGWKLNGQF MAB_0426|M.abscessus_ATCC_1997 TRTPATSYGSAVVYGYRKGACGKLCELHVDEANKLLDEAHFDR------- . . : Mvan_3773|M.vanbaalenii_PYR-1 -----PRELDMLVTTEYPETVTAAQIIADNLAP-LGISVKIRTVDFATWL Mflv_2761|M.gilvum_PYR-GCK -----PGDLDMLVTTEYPETVTAAQIVADNLAP-LGITVNIRTVDFATWL MSMEG_4354|M.smegmatis_MC2_155 -----PENLDMLVTSEYPETVTAAQIIADNLAP-LGISVNIRTVDFATWL TH_4152|M.thermoresistible__bu ---DPPRRLDMLVTGDYPQTVTAAQIIADNVAP-LGITVDIRTVDFATWL Mb1311c|M.bovis_AF2122/97 REKDGRQLVIRDLFYDAQSTRQFAQIAQHTLAQ-IGVKLELQAKSGSGFF Rv1280c|M.tuberculosis_H37Rv REKDGRQLVIRDLFYDAQSTRQFAQIAQHTLAQ-IGVKLELQAKSGSGFF MMAR_4139|M.marinum_M REKDGHQLVVRLLFYEAQSSRQFAQVAQYSLAQ-IGVKLELQSRSGSGFF MUL_4002|M.ulcerans_Agy99 REKDGHQLVVRLLFYEAQSSRQFAQVAQYSLAQ-IGVKLELQSRSGSGFF MAV_1429|M.avium_104 REKDGRQLVIRDLFYDAQGSRQFAQIAQHSLAQ-IGVRLELVARAGSGFF MLBr_01121|M.leprae_Br4923 REKDGRQLVIRYLFYDAQGSREFAQLAQHSLAQ-IGVKLELQAKAGSGFF MAB_0426|M.abscessus_ATCC_1997 -----NKPIELWYSSSSTGALSWVQAIGNQLQSNLKVPYLLKSLPWSQFL : . : .* : : : : : : :: Mvan_3773|M.vanbaalenii_PYR-1 DE-QNSGNFDMLMMGWLGNID-----PDDFYYAQHHTDGTSNAQKFSDPE Mflv_2761|M.gilvum_PYR-GCK DE-QNSGNFDMLMMGWLGNID-----PDDFYYAQHHTDGTSNAQKFSNPE MSMEG_4354|M.smegmatis_MC2_155 DE-QNTGNFDMLMMGWLGNID-----PDDFYYAQHHSNGTSNAQKFSDPE TH_4152|M.thermoresistible__bu DE-QNNGTFDMLMMGWLGNID-----PDDYYYAQHHTDGASNAQRFSDPE Mb1311c|M.bovis_AF2122/97 SDYVNVGAFDIAQFGWVGDAF-----PLSSLTQIYASDGESNFGKIGSPQ Rv1280c|M.tuberculosis_H37Rv SDYVNVGAFDIAQFGWVGDAF-----PLSSLTQIYASDGESNFGKIGSPQ MMAR_4139|M.marinum_M SNYVNVGAFDIALFGWVGDAF-----PLSSLTQIYMSDGESNFGKIGSPQ MUL_4002|M.ulcerans_Agy99 SNYVNVGAFDIALFGWVGDAF-----PLSSLTQIYMSDGESNFGKIGSPQ MAV_1429|M.avium_104 TNYINVGAFDMAQFGWLGDAF-----PLSALTQIYKSDGESNFGKIGSPQ MLBr_01121|M.leprae_Br4923 TNYVNVGAFDIVQFGWVGDAF-----PLSALTQIYQSDGESNFGKISSPA MAB_0426|M.abscessus_ATCC_1997 PLQDGKGMTGPFRSGWVMDYPSIYNFLESLYGTVALPPNGSNYVFYSNPA . * . **: : . . . ** ..* Mvan_3773|M.vanbaalenii_PYR-1 VDRLLDASRVETDEAARKEQYAGAATLIADKVSYIYLYNPSVIQAWNPAL Mflv_2761|M.gilvum_PYR-GCK VDRLLDAGRVETDEQTRKAGYARAATLIADEVSYIYLYNPSVIQAWNPAL MSMEG_4354|M.smegmatis_MC2_155 VDRLLDAGRVETDTAERKDVYARAATRIADEVSYIYLYNPSVIQAWSNDL TH_4152|M.thermoresistible__bu VDRLLDAARAEPDRDRRKALYDRAATLIADRVSYLFLYNPAVVQAWSPDL Mb1311c|M.bovis_AF2122/97 IDAAIERTLAELDPGKARALANQVDELIWAEGFSLPLTQSPGTVAVRSTL Rv1280c|M.tuberculosis_H37Rv IDAAIERTLAELDPGKARALANQVDELIWAEGFSLPLTQSPGTVAVRSTL MMAR_4139|M.marinum_M IDAAIERTLQELDPAKALALANEVDKLIWAEGFSLPLTQSPGTIAVRSTL MUL_4002|M.ulcerans_Agy99 IDAAIERTLQELDPAKALALANQVDKLIWAEGFSLPLTQSPGTIAVRSTL MAV_1429|M.avium_104 IDAAIERTLQELDPDKARALANDLDKLIWAEGFSLPLTQSPGDVAVRSTL MLBr_01121|M.leprae_Br4923 VDAAIERTLAELDPAKARALANEVDKLLWAEGFSLPLTQSPGDVAVRSTL MAB_0426|M.abscessus_ATCC_1997 FDELLRQGNNEPTVELATKAYQKAEDLLFKDLPAAPLFYEVNQAVHSKRV .* : * : * . : Mvan_3773|M.vanbaalenii_PYR-1 SGYEARRDGAVRFRSATFGADGGA----- Mflv_2761|M.gilvum_PYR-GCK SGYEARRDGAVRFRSASLDSDGNV----- MSMEG_4354|M.smegmatis_MC2_155 SGYVARRDGAIRFRTADLGRGGPE----- TH_4152|M.thermoresistible__bu SGYRARRDAAVRFRDASLNRDGLNRDGPP Mb1311c|M.bovis_AF2122/97 ANFGATGLADLDYTAIGFMRR-------- Rv1280c|M.tuberculosis_H37Rv ANFGATGLADLDYTAIGFMRR-------- MMAR_4139|M.marinum_M ANFGARGLADLDYTAIGFMRS-------- MUL_4002|M.ulcerans_Agy99 ANFGARGLADLDYTAIGFMRS-------- MAV_1429|M.avium_104 ANFGAAGLGDLNYTAIGFTRS-------- MLBr_01121|M.leprae_Br4923 ANFGAAGFGDLNYTAIGFMQN-------- MAB_0426|M.abscessus_ATCC_1997 RNVKIGIQDCIEYADVEVVQ--------- . : : .