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LEGTIHIAVTGGIGYLGAHTVRALLEAGHSVRLLVLPQEQDAPVIDRLRALGEVSVVVGDIRSESTVTEL LSGADAVLHAAGVVGTDERRSQLMWDINAYATEQILTRAVDLGLDPVVSVSSYSALFPPKNGVISADSPT ADGRSSYAKTKGYADRAARRLQDAGAPVVVTYPSSVVGAAFGGTASGVTERGWAPIVRARVAPRVRAGMQ MIDVRDVADVHTRLMSPGRGPHRYVCGGVMLTFDEMIDALEEGLGRPVRRIPMSPKALLAVGRISDVAGR YVPLGDGLSYEAALLLTSATPTDDSVTRQDLGLSWRSPRAAIVESFAAARVSAGRGIA
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1564 | - | - | 100% (338) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_3154 | - | 3e-53 | 36.17% (329) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_1108 | - | 2e-11 | 22.99% (335) | NAD-dependent epimerase/dehydratase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3752 | - | 5e-57 | 36.86% (331) | putative oxidoreductase |
| M. tuberculosis H37Rv | Rv3725 | - | 3e-45 | 39.84% (251) | oxidoreductase |
| M. leprae Br4923 | MLBr_01942 | - | 2e-09 | 28.57% (161) | putative cholesterol dehydrogenase |
| M. abscessus ATCC 19977 | MAB_2971c | - | 7e-58 | 38.87% (337) | putative oxidoreductase |
| M. marinum M | MMAR_5252 | - | 2e-58 | 38.65% (326) | oxidoreductase |
| M. avium 104 | MAV_5157 | - | 3e-13 | 24.11% (336) | 3-beta hydroxysteroid dehydrogenase/isomerase family protein |
| M. smegmatis MC2 155 | MSMEG_5909 | - | 1e-129 | 69.02% (326) | oxidoreductase |
| M. thermoresistible (build 8) | TH_1114 | - | 2e-10 | 30.13% (156) | PROBABLE CHOLESTEROL DEHYDROGENASE |
| M. ulcerans Agy99 | MUL_4328 | - | 1e-58 | 38.65% (326) | oxidoreductase |
| M. vanbaalenii PYR-1 | Mvan_5194 | - | 1e-150 | 85.44% (309) | NAD-dependent epimerase/dehydratase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_1564|M.gilvum_PYR-GCK ----------MEGTIHIAVTGGIGYLGAHTVRALLEAGHSVRLLVLPQEQ
Mvan_5194|M.vanbaalenii_PYR-1 ------------------------------MRALLEAQHTIRLLVLPQEQ
MSMEG_5909|M.smegmatis_MC2_155 ---------MSEESVRIAVTGGTGYVGAHTVRGLLTAGHEVRLLVAPGCE
MMAR_5252|M.marinum_M ---------MHNRCMHVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPAK
MUL_4328|M.ulcerans_Agy99 ---------MHNRCMHVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPAK
Mb3752|M.bovis_AF2122/97 ---------MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPAR
Rv3725|M.tuberculosis_H37Rv ---------MQNATMRVLVTGGTGFVGGWTAKAIADAGHSVRFLVRNPAR
MAB_2971c|M.abscessus_ATCC_199 --------------MRVLVTGGTGFVGGWTAKAMAEAGHKVRFLVRKEAR
MLBr_01942|M.leprae_Br4923 MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAP--
TH_1114|M.thermoresistible__bu ----MGDPTLRTDLGRVLVTGGSGFVGTNLVTTLLERGHHVRSCDRVG--
MAV_5157|M.avium_104 -----------MSGKPKLVIGANGFLGSHVTRQLVADGAQVRAMVRAGAN
: :*
Mflv_1564|M.gilvum_PYR-GCK DAPVIDRLRALGEVSVVVGDIRSESTVTELLSGADAVLHAAGVVGTD---
Mvan_5194|M.vanbaalenii_PYR-1 DAPVIRRLRSLGDVSVIVGDVRAESTVTALLTGVDAVVHAAGVVGTD---
MSMEG_5909|M.smegmatis_MC2_155 GEPVIDKLAEAGEVETLVGDIRDSGTVDRLIKGCDSVIHAAGVVGTD---
MMAR_5252|M.marinum_M LESSVAKLG-VDVSDFIIGDIKDRDLVREALTGCDAVVHSAALVATD---
MUL_4328|M.ulcerans_Agy99 LESSVAKLG-VDVSDFIIGDIKDRDLVREALTGCDAVVHSAALVATD---
Mb3752|M.bovis_AF2122/97 LKTSVAKLG-VDVSDFAVADISDRDSVREALNGCDAVVHSAALVATD---
Rv3725|M.tuberculosis_H37Rv LKTSVAKLG-VDVSDFAVADISDRDSVREALNGCDAVVHSAALVATD---
MAB_2971c|M.abscessus_ATCC_199 LHTTVATLG-VDVSDYAVGDITDRASVEAALDGCDAVVHSAAMVSTD---
MLBr_01942|M.leprae_Br4923 -----MPLPQHPQLEVLQGDITDATVCTTAMDSIDTVFHTAAIIELMGGA
TH_1114|M.thermoresistible__bu -----SPLPDHPRLEVIDGDICDADAVAAAVDGIDTVFHTAAVIDLMGGA
MAV_5157|M.avium_104 TRG-IDDLS----LTRFHGDVFDTAVLSEAMDGVDDVYYCVVDTRAW---
* .*: : . * * : .
Mflv_1564|M.gilvum_PYR-GCK ---ERRSQLMWDINAYATEQILTRAVDL-GLDPVVSVSSYSALFPPKNGV
Mvan_5194|M.vanbaalenii_PYR-1 ---ERRAQLMWDINAYATEQVLTRAVEL-GLDPVVSVSSYSALFPPPDGI
MSMEG_5909|M.smegmatis_MC2_155 ---KSRTQLMWEINAHATEAVLIRAAEA-GLDPIVSVSSYSSLFPPPDGV
MMAR_5252|M.marinum_M ---QRQTQDMLSTNMEGARNVLGQAVAL-GLDPIVHVSSFTALFHPGLQT
MUL_4328|M.ulcerans_Agy99 ---QRQTQDMLSTNMEGARNVLGQAVAL-GLDPIVHVSSFTALFHPGLQT
Mb3752|M.bovis_AF2122/97 ---PRETSRMLSTNMAGAQNVLGQAVEL-GMDPIVHVSSFTALFRPNLAT
Rv3725|M.tuberculosis_H37Rv ---PRETSRMLSTNMAGAQNVLGQAVEL-GMDPIVHVSSFTALFRPNLAT
MAB_2971c|M.abscessus_ATCC_199 ---PAEADRMMAINLEGTRNVLGAAVDR-GLDPIVHVSSITALFRPGLET
MLBr_01942|M.leprae_Br4923 SVTDEYRQRSYTVNVGGTENLLRAGQKS-GVKRFVYTASNSVVMGGTPIT
TH_1114|M.thermoresistible__bu GVTDEYRERSFAVNVHGTERLVRAAQQA-GVRRFVYTASNSVVMGGQPIA
MAV_5157|M.avium_104 ---VRDTSPLFRTNVEGLRNVLDVAVTQPELRKFIFTSTYATVGRRRGHV
. * . . :: . : .: .:: : :
Mflv_1564|M.gilvum_PYR-GCK ISADSPTADG--RSSYAKTKGYADRAARRLQDAGAP----VVVTYPSSVV
Mvan_5194|M.vanbaalenii_PYR-1 ISADSPTSSG--RSSYAKTKGYADRVARRLQDAGAP----VVVTYPSSVV
MSMEG_5909|M.smegmatis_MC2_155 ISADTPPVAG--RSPYAQTKAYADRAARRLQDTGAP----VVVTYPSSVV
MMAR_5252|M.marinum_M LSADLPVVGG--SDGYGTSKAQVEIYARGLQDAGAP----VNITYPGMVL
MUL_4328|M.ulcerans_Agy99 LSADLPVVGG--SDGYGTSKAQVEIYARGLQDAGAP----VNITYPGMVL
Mb3752|M.bovis_AF2122/97 LSADLPVAGG--TDGYGQSKAQIEIYARGLQDAGAP----VNITYPGMVL
Rv3725|M.tuberculosis_H37Rv LSADLPVAGG--TDGYGQSKAQIEIYARGLQDAGAP----VNITYPGMVL
MAB_2971c|M.abscessus_ATCC_199 FAADLPAAGG--SDGYGQSKARVELYVRALQDSGAP----VTITYPGMVL
MLBr_01942|M.leprae_Br4923 GGDETMPYTKRFNDLYTETKVVAEKFVLSQNGVPDGETMLTCSIRPSGIW
TH_1114|M.thermoresistible__bu DGDETLPYTDRFNDLYTETKVIAERFVLGQNGIEG---MLTCSIRPSGIW
MAV_5157|M.avium_104 ATEDDVIGTRG-LSDYVKSRVQAENLVMRYVAEAGLP---AVAMCVSTTY
: . * :: : . . .
Mflv_1564|M.gilvum_PYR-GCK G---AAFGGTASGVTERGWAPIVRARVAPRVRAGMQMIDVRDVADVHTRL
Mvan_5194|M.vanbaalenii_PYR-1 G---PAFG-TAAGVTERGWEPIVRARVAPRVRAGMQMIDVRDVADVHVRL
MSMEG_5909|M.smegmatis_MC2_155 G---PAWF-TAPGVTERGWGPIVKARVAPRLRGGMQMIDVRDVADVHVAL
MMAR_5252|M.marinum_M G---PPVG-NQFGEAGEGVKAALEIRTIPGRNAAWLIIDVRDVAAVHAAL
MUL_4328|M.ulcerans_Agy99 G---PPVG-NQFGEAGEGVKAALEIRTIPGRNAAWLIIDVRDVAAVHAAL
Mb3752|M.bovis_AF2122/97 G---PPVG-DQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAAL
Rv3725|M.tuberculosis_H37Rv G---PPVG-DQFGEAGEGVRSALWMHVIPGRGAAWLIVDVRDVAALHAAL
MAB_2971c|M.abscessus_ATCC_199 G---PPVG-DQFGEAAEGVRWVLLTRSVPGRSAAWLVVDVRDLAALHTAL
MLBr_01942|M.leprae_Br4923 GRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSYVHNLVHGLILAAEHL
TH_1114|M.thermoresistible__bu GPGDQTMFRKLFESVHAGHVKVLIGSRHARLDNSYVHNLVHGFILAAEHL
MAV_5157|M.avium_104 G------SGDWGRTPHGAFIAGAVFGKLPFTMEGIQLEVVGVTDAAKAMV
* . . . * :
Mflv_1564|M.gilvum_PYR-GCK MSPGRGP--HRYVCGGVMLTFDEMIDALEEGLGRPVRRIPMSPKALLAVG
Mvan_5194|M.vanbaalenii_PYR-1 MRPGRGP--RRYVCGGVMLTFDEMIDALEAGLGRPVRRVPLSPKVLLAVG
MSMEG_5909|M.smegmatis_MC2_155 MKPGRGP--HRYVCGGVLVSFNEWIDILEQGVGRPIRRIPLSPAMFHAIG
MMAR_5252|M.marinum_M LEPGRGP--RRYMVGGHRIPVPELAKMLGQVAETPIVSVPIPDSVLRVAG
MUL_4328|M.ulcerans_Agy99 LEPGRGP--RRYMVGGHRIPVPELAKMLGQVAETPIVSVPIPDSVLRVAG
Mb3752|M.bovis_AF2122/97 LESGRGP--RRYTAGGHRIPVPELAKILGEVAGTTMLAVPVPDSALRVAG
Rv3725|M.tuberculosis_H37Rv LESGRGP--RRYTAGGHRIPVPELAKILGGSPAPRCWPSRCPIPRCVSRD
MAB_2971c|M.abscessus_ATCC_199 LEPSRGP--RRYTAGGHRLEVGELVDLLTEAAGSAVLAVPVPDSLLRAAG
MLBr_01942|M.leprae_Br4923 VPGGTAPGQAYFINDGEPINFFDFMGPIIKACGENWPRVRISGRLVRNVM
TH_1114|M.thermoresistible__bu VPGGTAPGQAYFINDGEPINMFEFARPVVTACGQRWPTLRVSGPLVRAVM
MAV_5157|M.avium_104 LAADRGRVGERYLISERMIALKEVVRIAADEAGVPPPRRSISVPTLYALG
: . . : . : . : .
Mflv_1564|M.gilvum_PYR-GCK RISDVAGRYVPLGDGLSYEAALLLTSAT-PTDDSVTRQDLGL-SWRSPRA
Mvan_5194|M.vanbaalenii_PYR-1 RLSDAVGRYVPLGDGLSYEAALLLTSAT-PTDDRATTEDLGL-HWRSPRQ
MSMEG_5909|M.smegmatis_MC2_155 WVSDLLGNVLPLGDGISYEAAMLLTAAT-PTDDAKTLADLGIPAWRSPRA
MMAR_5252|M.marinum_M SLLDLAGPYLPFDNPFTAAGMQYYTQMP-DSDDSPSELELGVTYRDP-RT
MUL_4328|M.ulcerans_Agy99 SLLDLAGPYLPFDNPFTAAGMQYYTQMP-DSDDSPSELELGVTYRDP-RT
Mb3752|M.bovis_AF2122/97 SVLDQAGPYLPFNTPFTAAGMQYYTQMP-EPDDSPSEKELGITYRDP-RD
Rv3725|M.tuberculosis_H37Rv RCWIKPGP-------------ICLSILR-SPRQVCS-----TTHRCR-SP
MAB_2971c|M.abscessus_ATCC_199 TVMDQIGRYVPWETPMTEAGMQYYTQMP-ASDDTPSERELGITYRDP-RE
MLBr_01942|M.leprae_Br4923 AVWQRLHFGFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGYRPLFTTEQ
TH_1114|M.thermoresistible__bu SLWQRLHFRFGLPKPPLEPLAVERLYLDNYFSIDKARRELGYRPRYTTEQ
MAV_5157|M.avium_104 ALGDLRARLTGKDAELSLASVRMMRAEA-PVDHSKAVRELGWQPRPVEES
:
Mflv_1564|M.gilvum_PYR-GCK AIVESFAAARVSAGRGIA----------------
Mvan_5194|M.vanbaalenii_PYR-1 AIIESFGVAGR-LGDPVS----------------
MSMEG_5909|M.smegmatis_MC2_155 AVLESFGR--------------------------
MMAR_5252|M.marinum_M TLADTVAALTA-----------------------
MUL_4328|M.ulcerans_Agy99 TLADTVAALTA-----------------------
Mb3752|M.bovis_AF2122/97 TVADTVTALRGLGS--------------------
Rv3725|M.tuberculosis_H37Rv TIRRAKKN--------------------------
MAB_2971c|M.abscessus_ATCC_199 TLADTVVSLRAGRTTSKLWGLWPFSE--------
MLBr_01942|M.leprae_Br4923 AMAECLPYYTELFEQIKVAAKPHLASIAATPRPE
TH_1114|M.thermoresistible__bu ALQECLPYYVDLFHRMKAEP----ATV-------
MAV_5157|M.avium_104 IREAARFWAAMRNAKGKSATPR------------