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SGAPHVAVALEGYGWHPQAWRHSAGAPPVTSGGYWAELVATAERGLLDFVTFDDAPAPQRRRRPEIDPRW LAGRPDAVLVAARVAPLTRHIGLIRVATTTHTEPAALSESLATLDHLSQGRAGWQVRISASRHEAQLFGR RDPDDLGDLFAHARGVVEIVRTRWEGGGGRPVVTALAHTPTVYEFAADSADLVFVTPRDDESLSTILAEV REAGGHELRVYADLYVTFTGVVDERSDARVFSGTADELADTIAGWHRHGVHGVRLRPAVNAVDLPVIADE VIPLLQCRTGFRSTYRPGETLRGRLGIES*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0216 | - | - | 100% (310) | fmnh2-utilizing oxygenase |
| M. gilvum PYR-GCK | Mflv_0214 | - | 5e-13 | 30.49% (305) | fmnh2-utilizing oxygenase |
| M. gilvum PYR-GCK | Mflv_0250 | - | 1e-11 | 33.63% (113) | hypothetical protein Mflv_0250 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | - | - | - | - | - |
| M. tuberculosis H37Rv | - | - | - | - | - |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4234c | - | 5e-66 | 44.06% (345) | putative FMNH2-utilizing oxygenase |
| M. marinum M | MMAR_0701 | - | 3e-88 | 50.85% (352) | oxidoreductase |
| M. avium 104 | MAV_2050 | - | 7e-92 | 52.38% (357) | fmnh2-utilizing oxygenase |
| M. smegmatis MC2 155 | MSMEG_2177 | - | 1e-57 | 38.34% (373) | fmnh2-utilizing oxygenase |
| M. thermoresistible (build 8) | TH_4079 | - | 4e-16 | 34.31% (137) | PUTATIVE nitrilotriacetate monooxygenase component A |
| M. ulcerans Agy99 | - | - | - | - | - |
| M. vanbaalenii PYR-1 | Mvan_6020 | - | 2e-12 | 27.88% (165) | xenobiotic compound DszA family monooxygenase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_0701|M.marinum_M ------MTRPLHLGVALDGYGWHPQAWQTTLAADAQTRSVLSGDYWADLA
MAV_2050|M.avium_104 ------MT-ELHLAVALDGYGWHPQAWRYAFAHNATGGGQFSGRYWAALA
Mflv_0216|M.gilvum_PYR-GCK ------MSGAPHVAVALEGYGWHPQAWR----HSAGAPPVTSGGYWAELV
MAB_4234c|M.abscessus_ATCC_199 --MGVAPTVTTLLGLALDGHGWHPDADK-------HAGKPLTGRYWTQQV
MSMEG_2177|M.smegmatis_MC2_155 MATPSDPAHLPRLAVALDGTGWHPASWR---EPDARPAEVLRAGYWTDLI
TH_4079|M.thermoresistible__bu -----------LPETRLSG--------------------ITDIDHYLELA
Mvan_6020|M.vanbaalenii_PYR-1 ---------MMHLITWLMGNSYHAAGWR---HQLAWERTAMRLDVLIEIA
* *
MMAR_0701|M.marinum_M ITAERGLLDFLTIDDTLMPQPGR----RERIDPKRLAGRADAVLVAARIA
MAV_2050|M.avium_104 RTAERGLLDFLTIDDSLDAQPGR----RAEISPRRLVGRADATLVAARVA
Mflv_0216|M.gilvum_PYR-GCK ATAERGLLDFVTFDDAPAPQRRR----RPEIDPRWLAGRPDAVLVAARVA
MAB_4234c|M.abscessus_ATCC_199 QLAEQGLLDFVTFEDSFAPTPG---------------GRLDAVLTAGRVA
MSMEG_2177|M.smegmatis_MC2_155 GAAEHGLIDFVTIEDGLTLQSDHPF--RPDDRTDRLRGRLDAVLIASRVA
TH_4079|M.thermoresistible__bu RIAERGKLDAIFFADHPVLKGKTEFRPFDA---------LDPVTLVTALS
Mvan_6020|M.vanbaalenii_PYR-1 KIAEEAKLDALFVADGNGVRNMDKVGLFEANTPSARPTVYDPVTLMAAIS
**.. :* : . * *.. ::
MMAR_0701|M.marinum_M PLTRHIGLIPVATVTHTEPFHISKAIATLDYVSHGRAGWQARVSATTHEA
MAV_2050|M.avium_104 PVTEHIGLIPVATVTHTEPFHVSKAIATLDHISHGRAGWQVRVSATEQEA
Mflv_0216|M.gilvum_PYR-GCK PLTRHIGLIRVATTTHTEPAALSESLATLDHLSQGRAGWQVRISASRHEA
MAB_4234c|M.abscessus_ATCC_199 PATHHIGLIPTAPATHNEPFHLSKSIATLDIVSGGRAGWQVTIADTEDEA
MSMEG_2177|M.smegmatis_MC2_155 PTTRHIGLIPTAVTTFTEPFHISKAVATLDYVSTGRAGVRVQVSSRRDAA
TH_4079|M.thermoresistible__bu TATTHIGLVATASTTYTEPYGLARRFATLDHVSRGRAGWNIVTTANAAAA
Mvan_6020|M.vanbaalenii_PYR-1 QHTKHIGLVGTASTTYESPWVVARRFASLDHLSNGRASWNVVTGSNPGDS
* ****: .* .*. .* ::. .*:** :* ***. . :
MMAR_0701|M.marinum_M ALFGRRIVRR--GESSAEAFGEARDFVEVVRKLWDSWEDDAVIRDVTTGR
MAV_2050|M.avium_104 ALFGRRAVP-----GVDELFDEAADAVEVARRLWDSWEDDAVIRDVATGR
Mflv_0216|M.gilvum_PYR-GCK QLFGRRDPD-----DLGDLFAHARGVVEIVRTRWEGGG------------
MAB_4234c|M.abscessus_ATCC_199 DAFGRTKVL-----PLQDLLDEAADSVEVVRRLWDSWEDDAEIRDVATGR
MSMEG_2177|M.smegmatis_MC2_155 AHFGRRQFPDDRAALVAELFGEATDYVEVLRRLWDSWEDDAEIRDVSTGR
TH_4079|M.thermoresistible__bu ANFGRPAHP-----DPEHRYSRADEFLQVALRLWDSWEDDAIVGDKQAPR
Mvan_6020|M.vanbaalenii_PYR-1 ENFGLAHHP-----DRDSRYARAEEFVSACKALWNSWDEDAFVERKDTGQ
** .* :. *:.
MMAR_0701|M.marinum_M YIDRDKVHYIDFVGTYFSVKGPSITPRPPQGQPVVAALAHAGPIYEFAAA
MAV_2050|M.avium_104 YIDRDKLHYIDFAGKYFSVKGPSITPRPPQGQPVVAALAHTPPVYEFAAA
Mflv_0216|M.gilvum_PYR-GCK ------------------------------GRPVVTALAHTPTVYEFAAD
MAB_4234c|M.abscessus_ATCC_199 FIDRDKLHYIDFEGRFFSVKGPSITPRSPQGQAVVAALARSRPGQAFAAK
MSMEG_2177|M.smegmatis_MC2_155 FIDRDKLHYIDFVGAHFSVKGPSITPRPPQGQPVVAALGHVDAAYRLIAT
TH_4079|M.thermoresistible__bu FVDPDKIHAIDHRGEHFSVAGPLEVPRSPQGHPVLFQAGSSEPGKQLAAK
Mvan_6020|M.vanbaalenii_PYR-1 YLNARKVRVPDYKGRHLSVKGPLNVSRSPQGRPVLFHAGQSEGGRRLAAR
*:.*: . : *
MMAR_0701|M.marinum_M SADVVFITPTDEDSLRSILGEV-------RATGVALKVFADVVVSLHDG-
MAV_2050|M.avium_104 NADLVFVTPREDDSLRAILAEVDRA---RRAAGRAIRVYADLVVSLVGHP
Mflv_0216|M.gilvum_PYR-GCK SADLVFVTPRDDESLSTILAEVR------EAGGHELRVYADLYVTFTGV-
MAB_4234c|M.abscessus_ATCC_199 NADLIFVTPHDGASAAEIPARLHN-----TAGHRVIKVLADLVVSFDRE-
MSMEG_2177|M.smegmatis_MC2_155 SADVGFITPHSTQEVTSLVETIAGH---RPADTEPVRVFADVVVFLDDTA
TH_4079|M.thermoresistible__bu YAEAIFTAQPTVEEARQFYADVKARVRQHGRDPDRVHILPGLSAIIGGTE
Mvan_6020|M.vanbaalenii_PYR-1 HADCIFEAAASVEAAREFYADLKRRRVEIGGEPDHLRIIVSVAVYLGRTE
*: * : : : ::: .: . :
MMAR_0701|M.marinum_M ---------------ADFRSD-----------------------------
MAV_2050|M.avium_104 L-----------AAAAAISSD-----------------------------
Mflv_0216|M.gilvum_PYR-GCK ---------------VDERSD-----------------------------
MAB_4234c|M.abscessus_ATCC_199 ---------------DEFPSD-----------------------------
MSMEG_2177|M.smegmatis_MC2_155 AGAQARRDRLDEVAGSEYVSD-----------------------------
TH_4079|M.thermoresistible__bu EEARRIQRRLEDLTVPDYGLEQLSRIVGYRVGRDDLDRRLQFGSDDPPAI
Mvan_6020|M.vanbaalenii_PYR-1 SEAVELYSELNSLISPDLGVDFLAKAVSEDLTGYPVDGPMPDLS--APVV
:
MMAR_0701|M.marinum_M --------------------------------ALVWAGSPTELVEMLLDW
MAV_2050|M.avium_104 --------------------------------ARVFAGSATDLAELVVAW
Mflv_0216|M.gilvum_PYR-GCK --------------------------------ARVFSGTADELADTIAGW
MAB_4234c|M.abscessus_ATCC_199 --------------------------------AAVFTGRAPELAELIAQW
MSMEG_2177|M.smegmatis_MC2_155 --------------------------------AEIFAGTPAQLADLVQQW
TH_4079|M.thermoresistible__bu HMQSRYALVRRLVSTENLTVRELLLRLSGGRGHRVFAGTPEQVADTIEEW
Mvan_6020|M.vanbaalenii_PYR-1 GGNSIRGQLDAIAKAEQLTVRQMYERVVPTMGNTALIGTATQIADVMEEW
* . ::.: : *
MMAR_0701|M.marinum_M RRLG-IDGARLRPAVNATDLAGIVDEVVPLLQRAGCFRTGYRDGETLRER
MAV_2050|M.avium_104 QDAG-VDGVRLRPAVNALDLPAIVDDLVPRLQRAGRFRTGYRDGETLRER
Mflv_0216|M.gilvum_PYR-GCK HRHG-VHGVRLRPAVNAVDLPVIADEVIPLLQCRTGFRSTYRPGETLRGR
MAB_4234c|M.abscessus_ATCC_199 HEWG-IDGVRLRPAANALDIPVVVDELVPLLQAHQGFRTEYENG-ALRER
MSMEG_2177|M.smegmatis_MC2_155 HAAG-ASGLRLRPATLPHDLRQITDGLVPELQRRGAFRDGYDTT-TLRGL
TH_4079|M.thermoresistible__bu FTTGAADGFNLIPPALPSSLTAFVDHVVPELQRRGLFRTEYTGT-TLRDH
Mvan_6020|M.vanbaalenii_PYR-1 YTTGACDGFVLGASISPFTLLLIRDELVPELQRRGLFRRHYTGS-TLREN
* * * .. . : . * ::* ** ** * :**
MMAR_0701|M.marinum_M LGLPPAPNRYAAVSR-----
MAV_2050|M.avium_104 LGLAPAPNRYATAAS-----
Mflv_0216|M.gilvum_PYR-GCK LGIES---------------
MAB_4234c|M.abscessus_ATCC_199 LGLPAAINRYARKSA-----
MSMEG_2177|M.smegmatis_MC2_155 LGLPRPANRYAAA-------
TH_4079|M.thermoresistible__bu LGLPRPRNRFTTGRPVAAAS
Mvan_6020|M.vanbaalenii_PYR-1 LGLPPVDN-----SPFAT--
**: