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PGSAMTALPAPPLMYFRSSLLRILFASGDRIFSDCRDRREGRDVTEPAALVFEDRGFSLPGLGAMADGLA AALRKQGVVAGDRVAVMSSNRPEFVAVLNVIWCLGASAVLISPAWKRAEVDHALALTGPAHAAGDHPVLA GLMPMLHLDEPITPAAPLPGAPRRAGDALLVFSSGTTGLPKAVRHTHASLNAAIGHWRDALGLTERDRIQ IVTPPSHILGLLNIVAALRTGVRVRLHRRFDIDQMLAHIESDRITVEMAVAPIALAMASHPNLESYDLSS LRFIMWGATPVSAGIAETVTNRTGIGWVPAYGTTELPVIACSPLRGARLDTVGRVLPGVDLRVVSTETGE RVGPGEVGEIQARSHLLMAGYLPAAATAEVMSQGWYRTGDIGSLDAAGWLRVTDRLKEMIKVRGFQVAPT EIEAVLHGHPAVRDCAVFGVPDGANGEAVVVAVAARTPVDAAELRARVAASLASYKRLSRIVFVPDIPRL PSGKVLRRALKELYGCTSDS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. ulcerans Agy99 | MUL_0362 | - | - | 100% (511) | acyl-CoA synthetase |
| M. ulcerans Agy99 | MUL_4126 | fadD3 | 2e-44 | 32.46% (496) | acyl-CoA synthetase |
| M. ulcerans Agy99 | MUL_4803 | fadD7 | 4e-41 | 30.83% (506) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3116 | fadD13 | 5e-48 | 30.46% (476) | chain-fatty-acid-CoA ligase |
| M. gilvum PYR-GCK | Mflv_2392 | - | 1e-174 | 67.17% (460) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3089 | fadD13 | 5e-48 | 30.46% (476) | chain-fatty-acid-CoA ligase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 8e-38 | 28.98% (490) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4055c | - | 2e-52 | 31.32% (479) | putative acyl-CoA synthetase |
| M. marinum M | MMAR_4793 | - | 0.0 | 98.07% (467) | acyl-CoA synthetase |
| M. avium 104 | MAV_0863 | - | 0.0 | 76.23% (467) | AMP-binding enzyme, putative |
| M. smegmatis MC2 155 | MSMEG_4876 | - | 1e-159 | 62.93% (464) | acyl-CoA synthase |
| M. thermoresistible (build 8) | TH_4258 | - | 0.0 | 68.80% (468) | PUTATIVE AMP-dependent synthetase and ligase |
| M. vanbaalenii PYR-1 | Mvan_4256 | - | 1e-178 | 68.33% (461) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3116|M.bovis_AF2122/97 --------------------------------------------MKNIGW
Rv3089|M.tuberculosis_H37Rv --------------------------------------------MKNIGW
MUL_0362|M.ulcerans_Agy99 ------------MPGSAMTALPAPPLMYFRSSLLRILFASGDRIFSDCRD
MMAR_4793|M.marinum_M --------------------------------------------------
MAV_0863|M.avium_104 --------------------------------------------------
Mflv_2392|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4256|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_4258|M.thermoresistible__bu --------------------------------------------------
MSMEG_4876|M.smegmatis_MC2_155 --------------------------------------------------
MAB_4055c|M.abscessus_ATCC_199 -------------------------------MTFRSPLPDVSIPDCSVYE
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mb3116|M.bovis_AF2122/97 MLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDR
Rv3089|M.tuberculosis_H37Rv MLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDR
MUL_0362|M.ulcerans_Agy99 RREGRDVTEPAALVFED----RGFSLPGLGAMADGLAAALRKQGVVAGDR
MMAR_4793|M.marinum_M ------MTEPAALVFED----RGFSLPGLGAMADGLAAALRKQGVVAGDR
MAV_0863|M.avium_104 ------MTEPAALVFEE----RRFSVPELDALADGWAAALAKDGVTAGRR
Mflv_2392|M.gilvum_PYR-GCK ------MSEPTALAFED----RCLSLADIDALAAGMAVELQRRGVGAGSR
Mvan_4256|M.vanbaalenii_PYR-1 ------MSDPWALAFED----RQYSLADLDALAGGMAAELHRRGVAAGSR
TH_4258|M.thermoresistible__bu ------MADRVALTFED----RAYSAAELDALTGGTAAALAARGVCPGSR
MSMEG_4876|M.smegmatis_MC2_155 ---MRLMGESAVLAFGD----REYTLTELDALAGGMAATLAQRGVGPGDR
MAB_4055c|M.abscessus_ATCC_199 YVFGDTGAEDSRIALIDGLTGAQTTLGELRSQIDATAAGLAERGFGIGDV
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALID--EEGTLTYKELDRAAHALANGLIAKGVRGGDG
. . : : : * * *. *
Mb3116|M.bovis_AF2122/97 VALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVI
Rv3089|M.tuberculosis_H37Rv VALLMPNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVI
MUL_0362|M.ulcerans_Agy99 VAVMSSNRPEFVAVLNVIWCLGASAVLISPAWKRAEVDHALALTGPAHAA
MMAR_4793|M.marinum_M VAVMSSNRPEFVAVLNAIWCLGASAVLISPAWKRAEVDHALALTGPAHAA
MAV_0863|M.avium_104 VAVMTSNRPEFLAVLLAIWRLAATAVLISPAWKRDEVEHALALTDPGHAV
Mflv_2392|M.gilvum_PYR-GCK VALMSSNRPEFVVALRAIWRLGAAAVLLSPAWKQTEVGHAVALTEATHAV
Mvan_4256|M.vanbaalenii_PYR-1 VAVMSSNRPEFVVALRAIWRLGASAVLLSPAWKQAEVGHALSLTEPTHAV
TH_4258|M.thermoresistible__bu VAIMSSNRPEFVVALRAIWSLGAAAVLLSPAWKRAEVDHALALTTPSHAV
MSMEG_4876|M.smegmatis_MC2_155 VAMMSSNRPEFVIALRAIWRLGAAAVLISPAWKTTEIAHALELTRANHAV
MAB_4055c|M.abscessus_ATCC_199 AAVFLPNCSAFAVVLHGILRAGGTASTVNVLYTAEELAKQLIDSKAQLIF
MLBr_02546|M.leprae_Br4923 IAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKVII
*:: * * .. :. :: .
Mb3116|M.bovis_AF2122/97 YGAPSAPVIDAIRAQADPPGTVTDWIG----ADSLAERLRSAAADEPAVE
Rv3089|M.tuberculosis_H37Rv YGAPSAPVIDAIRAQADPPGTVTDWIG----ADSLAERLRSAAADEPAVE
MUL_0362|M.ulcerans_Agy99 GDHPVLAGLMP--------------------MLHLDEPITPAAP--LPGA
MMAR_4793|M.marinum_M GDHPVLAGLMP--------------------MLHLDEPITPAAP--LPGA
MAV_0863|M.avium_104 GDHPVLAGLMP--------------------MLHLDEPVPPAQP--TAGA
Mflv_2392|M.gilvum_PYR-GCK GDHPVLADAMP--------------------MLSLDDEIAPRDP-EAAPD
Mvan_4256|M.vanbaalenii_PYR-1 GDHPVLAGSMP--------------------MLSLDEEIAPVAHGAVQPA
TH_4258|M.thermoresistible__bu GDHPVLADLMP--------------------MLHLDEPVTADPDGAGRPV
MSMEG_4876|M.smegmatis_MC2_155 GDQPLLAEHLP--------------------MVHLDEPITPGER--PGPA
MAB_4055c|M.abscessus_ATCC_199 TVSPLLSRALEAAEIAGIDAAGVITVDPVEGRLSLADIARPDLAPPQVTF
MLBr_02546|M.leprae_Br4923 YDDEYTKVVSKAEPALGKLRALGVNPDEEEPPGSTDETLAELIARSSSAP
:
Mb3116|M.bovis_AF2122/97 CGGDDN--LFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRL
Rv3089|M.tuberculosis_H37Rv CGGDDN--LFIMYTSGTTGHPKGVVHTHESVHSAASSWASTIDVRYRDRL
MUL_0362|M.ulcerans_Agy99 PRRAGD--ALLVFSSGTTGLPKAVRHTHASLNAAIGHWRDALGLTERDRI
MMAR_4793|M.marinum_M PRRAGD--ALLVFSSGTTGLPKAVRHTHASLNAAIGHWRDALGLTERDRI
MAV_0863|M.avium_104 PPRAGD--AVLVFSSGTTGLPKAVRHTHASLDEAVRHWREALRLTRRDRI
Mflv_2392|M.gilvum_PYR-GCK PDAGAD--AVFVFSSGTTGMPKAVRHTHASLAVAIRHWRDALGLTAADRM
Mvan_4256|M.vanbaalenii_PYR-1 PTPDAD--AVFVFSSGTTGMPKAVRHTHASLAVAVRHWRAALGLTSADRM
TH_4258|M.thermoresistible__bu PPEDAD--ALFVFSSGTTGMPKAVRHTHGALRTAVRHWRDALGLTAADRM
MSMEG_4876|M.smegmatis_MC2_155 VAPDAD--ALFVFSSGTTGMPKAVRHSHAGFAAAIEHWRDALGLTAADRM
MAB_4055c|M.abscessus_ATCC_199 DPATHL--AVLPYSSGTTGKAKGVMLTHHNLVANIAQAKHLYGVQRGDRV
MLBr_02546|M.leprae_Br4923 APKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVPFRAHEVT
.: :***** .*.. . . :
Mb3116|M.bovis_AF2122/97 LLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCIGG
Rv3089|M.tuberculosis_H37Rv LLPLPMFHVAALTTVIFSAMR-GVTLISMPQFDATKVWSLIVEERVCIGG
MUL_0362|M.ulcerans_Agy99 QIVTPPSHILGLLNIV-AALRTGVRVRLHRRFDIDQMLAHIESDRITVEM
MMAR_4793|M.marinum_M QIVTPPSHILGLLNIV-TALRTGVRVRLHRRFDIDQMLAHIESDRITVEM
MAV_0863|M.avium_104 QVATPPSHILGLLNLL-TALKTGACVRLHPRFDIDKVLHHIESDRVTVEM
Mflv_2392|M.gilvum_PYR-GCK QVMTPPSHILGLLNII-MALDTGAWIRLHRRFDIDAMLRHIESDRITIEM
Mvan_4256|M.vanbaalenii_PYR-1 QVMTPPSHILGLLNIV-MALDTGAWIRLHRRFDIDAMLRHIESDRITIEM
TH_4258|M.thermoresistible__bu QIMTPPSHILGLLNIV-MALDTGTWIRLHRRFDIDRMLHHIQADRITIEM
MSMEG_4876|M.smegmatis_MC2_155 QIMTPPSHILGLLNIA-MVLDTGAWMRLHPRFDVETMLRHIESDGITIEM
MAB_4055c|M.abscessus_ATCC_199 LAVLPFFHIYGLVVLLNVQLKLGAELVILPRFELDTFLGSIANYRVDHVF
MLBr_02546|M.leprae_Br4923 LLPAPMFHALGYLHAT-LAMFLGSTLVLRRRFKPATVLEDIEKHQATAMV
* * . : * : :*. . *
Mb3116|M.bovis_AF2122/97 AVPAILNFMRQVPE--FAELDAPDFRYFITGGAPMPEALIKIYAAKN-IE
Rv3089|M.tuberculosis_H37Rv AVPAILNFMRQVPE--FAELDAPDFRYFITGGAPMPEALIKIYAAKN-IE
MUL_0362|M.ulcerans_Agy99 AVAPIALAMASHPN--LESYDLSSLRFIMWGATPVSAGIAETVTNRTGIG
MMAR_4793|M.marinum_M AVAPIALAMASHPN--LESYDLSSLRFIMWGATPVSAGIAETVTNRTGIG
MAV_0863|M.avium_104 AVAPIALAIASHPD--LESYDLSSLRYIVWGATPVNAEVAQTVTRRTGVG
Mflv_2392|M.gilvum_PYR-GCK AVAPIALALSAHPR--LEDHDLSSLRYIMWCATPVTQSVAEAVTARTGVT
Mvan_4256|M.vanbaalenii_PYR-1 AVAPIALALSAHPG--LEEHDLSSLRYIMWCATPVTQTVAEAVSARTGAT
TH_4258|M.thermoresistible__bu AVAPIALALAGHPG--LESYDLSSLRYIMWCATPVTQSVADAVTARTGVG
MSMEG_4876|M.smegmatis_MC2_155 AVAPIALALAAHPG--LERFDLSSLRFVMWCATPVTRSVAEDITARAGIR
MAB_4055c|M.abscessus_ATCC_199 VAPPVAVVLAKHPD--VDKYDVSCLRSVFSGAAPLDEQLGNAVAARLNCR
MLBr_02546|M.leprae_Br4923 VVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRALEELGPV
... : : * . :* .. .: : : .
Mb3116|M.bovis_AF2122/97 VVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREH
Rv3089|M.tuberculosis_H37Rv VVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREH
MUL_0362|M.ulcerans_Agy99 WVPAYGTTELPVIACSPLRGAR---LDTVGRVLPGVDLRVVSTETGERVG
MMAR_4793|M.marinum_M WVPAYGTTELPVIACSPLRGAR---LDTVGRVLPGVDLRVVSIETGERVG
MAV_0863|M.avium_104 WLPAYGTTELPVIACNPIEDAR---LDTVGRPVPGVEVRVVSLESGEPAG
Mflv_2392|M.gilvum_PYR-GCK WVTAYGASELPVISCNDLQHAR---LDTVGRAVTGVGIRIVSLQTGEVLG
Mvan_4256|M.vanbaalenii_PYR-1 WVTAYGASELPVISCNDLQDAR---LDTVGRAVAGVDIRIVSLESGAPLG
TH_4258|M.thermoresistible__bu WIAAYGTTELPVIACNPLQGGR---LDTVGRPVPGVQVRIVSLDTGAPVG
MSMEG_4876|M.smegmatis_MC2_155 WLTAYGTTELPVIACNPLDDTR---IETVGKPVRGVRVRIA---------
MAB_4055c|M.abscessus_ATCC_199 VSQGYGMTELSPVSHLIPPDRPDIPLNSVGIPVPNSENKIIDTETGDEIE
MLBr_02546|M.leprae_Br4923 IYNMYGSTEIAFATIAGPKDLQIN-SATVGPVVKGVKVKILDDSGKEVPR
*. :* :.* :
Mb3116|M.bovis_AF2122/97 G-------EGEVVIKSDILLKEYWNRPEATRDAFD-NGWFRTGDIGEIDD
Rv3089|M.tuberculosis_H37Rv G-------EGEVVIKSDILLKEYWNRPEATRDAFD-NGWFRTGDIGEIDD
MUL_0362|M.ulcerans_Agy99 P-----GEVGEIQARSHLLMAGYLPAAATAEVMS--QGWYRTGDIGSLDA
MMAR_4793|M.marinum_M P-----GEVGEIQARSHSLMAGYLPAAATAEVMS--QGWYRTGDIGSLDA
MAV_0863|M.avium_104 A-----GEVGEIQARSASLMAGYLPGEATGEAIR--DGWYRTGDAGWLDT
Mflv_2392|M.gilvum_PYR-GCK A-----GEEGEIQVRSDSAMAGYLPDQWTAQAFS--DGWYRTGDVGTLDG
Mvan_4256|M.vanbaalenii_PYR-1 P-----GDEGEIQVRSDSAMAGYLPDHWTADAFC--DGWYRTGDVGSLDA
TH_4258|M.thermoresistible__bu P-----GEEGEVQARSASVMAGYLPDQ--PGAFV--EGWYRTGDVGYLDT
MSMEG_4876|M.smegmatis_MC2_155 -------DTGEIQVCSPSAMCGYLPESATGGAFD--DGWYRTGDIGFLDD
MAB_4055c|M.abscessus_ATCC_199 IPAEGESAPGELLVRGPNVMAGYLGNEEATAATIEPDGFLHTGDIAVVRA
MLBr_02546|M.leprae_Br4923 G------QVGRIFVGNAFPFEGYTGGGGKQIIDG----LLSSGDVGYFDE
*.: . : * :** . .
Mb3116|M.bovis_AF2122/97 EGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWG
Rv3089|M.tuberculosis_H37Rv EGYLYIKDRLKDMIISGGENVYPAEIESVIIGVPGVSEVAVIGLPDEKWG
MUL_0362|M.ulcerans_Agy99 AGWLRVTDRLKEMIKVRGFQVAPTEIEAVLHGHPAVRDCAVFGVPDGANG
MMAR_4793|M.marinum_M AGWLRVTDRLKEMIKVRGFQVAPAEIEAVLHGHPAVRDCAVFGVPDGANG
MAV_0863|M.avium_104 GGWLRITDRLKEMIKVRGFQVAPAEIDAVLQGHPAVADCAVFGVPDGLNG
Mflv_2392|M.gilvum_PYR-GCK EGWLRITDRAKEMIKVRGFQVAPAEVEAVLHGHPAVEDCAVFGIP-AADG
Mvan_4256|M.vanbaalenii_PYR-1 DGWLRITDRAKEMIKVRGFQVAPAEVEAVLHGHPAVEDCAVFGVP-AADG
TH_4258|M.thermoresistible__bu DGWLRLTDRAKDMVKVRGFQVAPAEVEAVLHEHPAVDDCAVFGVP-SPDG
MSMEG_4876|M.smegmatis_MC2_155 DGYLHVTDRAKEMVKVRGFQVAPAEVEAVLHGHPAVADCAVFGEPHDTDG
MAB_4055c|M.abscessus_ATCC_199 DGVVTIVDRLKELIKYKGYQVPPAELEALLLTHPGIGDAAVIGVPDPSSG
MLBr_02546|M.leprae_Br4923 HDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATAIGVDDKEWG
. : : .* .::: * :* *:*:: :: * : : :.:* *
Mb3116|M.bovis_AF2122/97 EIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGK
Rv3089|M.tuberculosis_H37Rv EIAAAIVVADQNE-VSEQQIVEYCGTRLARYKLPKKVIFAEAIPRNPTGK
MUL_0362|M.ulcerans_Agy99 EAVVVAVAARTP--VDAAELRARVAASLASYKRLSRIVFVPDIPRLPSGK
MMAR_4793|M.marinum_M EAVVAAVAARTP--VGDAELRARVAASLASYKRLSRIVFVPDIPRLPSGK
MAV_0863|M.avium_104 EAVVAAVAVSAP--VDVTELTALVRQKLASYKHLSRVVVVPEIPRLPSGK
Mflv_2392|M.gilvum_PYR-GCK EAIVAAVTLGHP--VDTDELADLVADRLASYKRPSRVVVVDEIPRLPSGK
Mvan_4256|M.vanbaalenii_PYR-1 EAIVAAVAASGP--LDTDDLVALVAERLAGYKRLSQVFVVDEIPRLPSGK
TH_4258|M.thermoresistible__bu EALVAAVTRRGE--VSADELLALVGERLASYKRPSRVVFVPEIPRLPSGK
MSMEG_4876|M.smegmatis_MC2_155 EAVVAAVATCAE--VSAEELIDLVGARLASYKRPRRIEFVTEIPRLPSGK
MAB_4055c|M.abscessus_ATCC_199 EIPKAFVVRTDDD-LTDEAVMAFVEQKVAPHKRIRQVEFIDAIPKSAAGK
MLBr_02546|M.leprae_Br4923 ARLRAFVVKKQDATIDEDAIKAYVRDHLARYKVPREVIFLEELPRNPTGK
. *. : : :* :* .: . :*: .:**
Mb3116|M.bovis_AF2122/97 ILKTVLREQYSATVPK-----------
Rv3089|M.tuberculosis_H37Rv ILKTVLREQYSATVPK-----------
MUL_0362|M.ulcerans_Agy99 VLRRALKEL---YGCTSDS--------
MMAR_4793|M.marinum_M VLRRALKEL---YGCASDS--------
MAV_0863|M.avium_104 VLRRVLKER---HGCTSDS--------
Mflv_2392|M.gilvum_PYR-GCK VLRRVLRERAMGAGAKSGGF-------
Mvan_4256|M.vanbaalenii_PYR-1 VLRRVLRERAMSARAESGGFGGRPSDQ
TH_4258|M.thermoresistible__bu VLRRVLKER---HGCPSDG--------
MSMEG_4876|M.smegmatis_MC2_155 VLRRVLKER---HGRPSDG--------
MAB_4055c|M.abscessus_ATCC_199 ILRKDLRARI-----------------
MLBr_02546|M.leprae_Br4923 VLKRELRDFEVH---------------
:*: *: