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RLMGESAVLAFGDREYTLTELDALAGGMAATLAQRGVGPGDRVAMMSSNRPEFVIALRAIWRLGAAAVLI SPAWKTTEIAHALELTRANHAVGDQPLLAEHLPMVHLDEPITPGERPGPAVAPDADALFVFSSGTTGMPK AVRHSHAGFAAAIEHWRDALGLTAADRMQIMTPPSHILGLLNIAMVLDTGAWMRLHPRFDVETMLRHIES DGITIEMAVAPIALALAAHPGLERFDLSSLRFVMWCATPVTRSVAEDITARAGIRWLTAYGTTELPVIAC NPLDDTRIETVGKPVRGVRVRIADTGEIQVCSPSAMCGYLPESATGGAFDDGWYRTGDIGFLDDDGYLHV TDRAKEMVKVRGFQVAPAEVEAVLHGHPAVADCAVFGEPHDTDGEAVVAAVATCAEVSAEELIDLVGARL ASYKRPRRIEFVTEIPRLPSGKVLRRVLKERHGRPSDG*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4876 | - | - | 100% (459) | acyl-CoA synthase |
| M. smegmatis MC2 155 | MSMEG_2360 | - | 3e-53 | 31.61% (484) | 4-coumarate:CoA ligase |
| M. smegmatis MC2 155 | MSMEG_2883 | - | 1e-51 | 31.83% (487) | acyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3591 | fadD3 | 2e-55 | 36.12% (490) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_2392 | - | 1e-179 | 68.53% (464) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv3561 | fadD3 | 6e-56 | 36.33% (490) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_02546 | fadD2 | 2e-39 | 30.08% (482) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_4055c | - | 5e-56 | 32.50% (480) | putative acyl-CoA synthetase |
| M. marinum M | MMAR_4793 | - | 1e-160 | 63.36% (464) | acyl-CoA synthetase |
| M. avium 104 | MAV_0863 | - | 1e-164 | 62.28% (464) | AMP-binding enzyme, putative |
| M. thermoresistible (build 8) | TH_4258 | - | 0.0 | 69.51% (469) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_0362 | - | 1e-159 | 62.93% (464) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_4256 | - | 1e-179 | 67.23% (473) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3591|M.bovis_AF2122/97 ----------------------------------------MINDLRTVPA
Rv3561|M.tuberculosis_H37Rv ----------------------------------------MINDLRTVPA
Mflv_2392|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4256|M.vanbaalenii_PYR-1 --------------------------------------------------
TH_4258|M.thermoresistible__bu --------------------------------------------------
MMAR_4793|M.marinum_M --------------------------------------------------
MUL_0362|M.ulcerans_Agy99 --------------MPGSAMTALPAPPLMYFRSSLLRILFASGDRIFSDC
MAV_0863|M.avium_104 --------------------------------------------------
MSMEG_4876|M.smegmatis_MC2_155 --------------------------------------------------
MAB_4055c|M.abscessus_ATCC_199 ---------------------------------MTFRSPLPDVSIPDCSV
MLBr_02546|M.leprae_Br4923 MTKLRHYLERGAAEVHYLRSIFESGALGFEPPLTYAAMAVAMRKWGDLAM
Mb3591|M.bovis_AF2122/97 ALDRLVRQLPDHTALIAED----RRFTSTELRDAVYGAAAALIALGVEPA
Rv3561|M.tuberculosis_H37Rv ALDRLVRQLPDHTALIAED----RRFTSTELRDAVYGAAAALIALGVEPA
Mflv_2392|M.gilvum_PYR-GCK --------MSEPTALAFED----RCLSLADIDALAAGMAVELQRRGVGAG
Mvan_4256|M.vanbaalenii_PYR-1 --------MSDPWALAFED----RQYSLADLDALAGGMAAELHRRGVAAG
TH_4258|M.thermoresistible__bu --------MADRVALTFED----RAYSAAELDALTGGTAAALAARGVCPG
MMAR_4793|M.marinum_M --------MTEPAALVFED----RGFSLPGLGAMADGLAAALRKQGVVAG
MUL_0362|M.ulcerans_Agy99 RDRREGRDVTEPAALVFED----RGFSLPGLGAMADGLAAALRKQGVVAG
MAV_0863|M.avium_104 --------MTEPAALVFEE----RRFSVPELDALADGWAAALAKDGVTAG
MSMEG_4876|M.smegmatis_MC2_155 -----MRLMGESAVLAFGD----REYTLTELDALAGGMAATLAQRGVGPG
MAB_4055c|M.abscessus_ATCC_199 YEYVFGDTGAEDSRIALIDGLTGAQTTLGELRSQIDATAAGLAERGFGIG
MLBr_02546|M.leprae_Br4923 LPAMPARRSPHRAALIDEE----GTLTYKELDRAAHALANGLIAKGVRGG
. : : : : . * * *. .
Mb3591|M.bovis_AF2122/97 DRVAIWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPV
Rv3561|M.tuberculosis_H37Rv DRVAIWSPNTWHWVVACLAIHHAGAAVVPLNTRYTATEATDILDRAGAPV
Mflv_2392|M.gilvum_PYR-GCK SRVALMSSNRPEFVVALRAIWRLGAAAVLLSPAWKQTEVGHAVALTEATH
Mvan_4256|M.vanbaalenii_PYR-1 SRVAVMSSNRPEFVVALRAIWRLGASAVLLSPAWKQAEVGHALSLTEPTH
TH_4258|M.thermoresistible__bu SRVAIMSSNRPEFVVALRAIWSLGAAAVLLSPAWKRAEVDHALALTTPSH
MMAR_4793|M.marinum_M DRVAVMSSNRPEFVAVLNAIWCLGASAVLISPAWKRAEVDHALALTGPAH
MUL_0362|M.ulcerans_Agy99 DRVAVMSSNRPEFVAVLNVIWCLGASAVLISPAWKRAEVDHALALTGPAH
MAV_0863|M.avium_104 RRVAVMTSNRPEFLAVLLAIWRLAATAVLISPAWKRDEVEHALALTDPGH
MSMEG_4876|M.smegmatis_MC2_155 DRVAMMSSNRPEFVIALRAIWRLGAAAVLISPAWKTTEIAHALELTRANH
MAB_4055c|M.abscessus_ATCC_199 DVAAVFLPNCSAFAVVLHGILRAGGTASTVNVLYTAEELAKQLIDSKAQL
MLBr_02546|M.leprae_Br4923 DGIAILARNHRWFVIANYGCARVGARIILLNSEFSGPQIKEVSEREGAKV
*: * : . .. :. :. : . .
Mb3591|M.bovis_AF2122/97 LFAAGLFLGADRAAGLDRAALPALRHVVRVPVEADDGTWDEFIATGAGAL
Rv3561|M.tuberculosis_H37Rv LFAAGLFLGADRAAGLDRAALPALRHVVRVPVEADDGTWDEFIATGAGAL
Mflv_2392|M.gilvum_PYR-GCK AVGDHPVLADAMP---------------------------MLSLDDEIAP
Mvan_4256|M.vanbaalenii_PYR-1 AVGDHPVLAGSMP---------------------------MLSLDEEIAP
TH_4258|M.thermoresistible__bu AVGDHPVLADLMP---------------------------MLHLDEPVTA
MMAR_4793|M.marinum_M AAGDHPVLAGLMP---------------------------MLHLDEPITP
MUL_0362|M.ulcerans_Agy99 AAGDHPVLAGLMP---------------------------MLHLDEPITP
MAV_0863|M.avium_104 AVGDHPVLAGLMP---------------------------MLHLDEPVPP
MSMEG_4876|M.smegmatis_MC2_155 AVGDQPLLAEHLP---------------------------MVHLDEPITP
MAB_4055c|M.abscessus_ATCC_199 IFTVSPLLSRALEA-------AEIAGIDAAGVITVDPVEGRLSLADIARP
MLBr_02546|M.leprae_Br4923 IIYDDEYTKVVSKAEPALG-----KLRALGVNPDEEEPPGSTDETLAELI
Mb3591|M.bovis_AF2122/97 DAVAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGK
Rv3561|M.tuberculosis_H37Rv DAVAARAAAVAPQDVSDILFTSGTTGRSKGVLCAHRQSLSASASWAANGK
Mflv_2392|M.gilvum_PYR-GCK RDP-EAAPDPDAGADAVFVFSSGTTGMPKAVRHTHASLAVAIRHWRDALG
Mvan_4256|M.vanbaalenii_PYR-1 VAHGAVQPAPTPDADAVFVFSSGTTGMPKAVRHTHASLAVAVRHWRAALG
TH_4258|M.thermoresistible__bu DPDGAGRPVPPEDADALFVFSSGTTGMPKAVRHTHGALRTAVRHWRDALG
MMAR_4793|M.marinum_M AAPLPGAP--RRAGDALLVFSSGTTGLPKAVRHTHASLNAAIGHWRDALG
MUL_0362|M.ulcerans_Agy99 AAPLPGAP--RRAGDALLVFSSGTTGLPKAVRHTHASLNAAIGHWRDALG
MAV_0863|M.avium_104 AQPTAGAP--PRAGDAVLVFSSGTTGLPKAVRHTHASLDEAVRHWREALR
MSMEG_4876|M.smegmatis_MC2_155 GER--PGPAVAPDADALFVFSSGTTGMPKAVRHSHAGFAAAIEHWRDALG
MAB_4055c|M.abscessus_ATCC_199 DLAPPQVTFDPATHLAVLPYSSGTTGKAKGVMLTHHNLVANIAQAKHLYG
MLBr_02546|M.leprae_Br4923 ARSSSAPAPKVRKRASIIILTSGTTGTPKGANRNTPASLAPLGGILSKVP
. : : :***** .*..
Mb3591|M.bovis_AF2122/97 ITSDDRYLCINPFFHNFGYKAGILACLQTGATLIPHVTFDPLHALRAIER
Rv3561|M.tuberculosis_H37Rv ITSDDRYLCINPFFHNFGYKAGILACLQTGATLIPHVTFDPLHALRAIER
Mflv_2392|M.gilvum_PYR-GCK LTAADRMQVMTPPSHILGLLNII-MALDTGAWIRLHRRFDIDAMLRHIES
Mvan_4256|M.vanbaalenii_PYR-1 LTSADRMQVMTPPSHILGLLNIV-MALDTGAWIRLHRRFDIDAMLRHIES
TH_4258|M.thermoresistible__bu LTAADRMQIMTPPSHILGLLNIV-MALDTGTWIRLHRRFDIDRMLHHIQA
MMAR_4793|M.marinum_M LTERDRIQIVTPPSHILGLLNIV-TALRTGVRVRLHRRFDIDQMLAHIES
MUL_0362|M.ulcerans_Agy99 LTERDRIQIVTPPSHILGLLNIV-AALRTGVRVRLHRRFDIDQMLAHIES
MAV_0863|M.avium_104 LTRRDRIQVATPPSHILGLLNLL-TALKTGACVRLHPRFDIDKVLHHIES
MSMEG_4876|M.smegmatis_MC2_155 LTAADRMQIMTPPSHILGLLNIA-MVLDTGAWMRLHPRFDVETMLRHIES
MAB_4055c|M.abscessus_ATCC_199 VQRGDRVLAVLPFFHIYGLVVLLNVQLKLGAELVILPRFELDTFLGSIAN
MLBr_02546|M.leprae_Br4923 FRAHEVTLLPAPMFHALGYLHAT-LAMFLGSTLVLRRRFKPATVLEDIEK
. : * * * : * : *. * *
Mb3591|M.bovis_AF2122/97 HRITVLPGPPTIYQSLLDHPA--RKDFDLSSLRFAVTGAATVPVVLVERM
Rv3561|M.tuberculosis_H37Rv HRITVLPGPPTIYQSLLDHPA--RKDFDLSSLRFAVTGAATVPVVLVERM
Mflv_2392|M.gilvum_PYR-GCK DRITIEMAVAPIALALSAHPR--LEDHDLSSLRYIMWCATPVTQSVAEAV
Mvan_4256|M.vanbaalenii_PYR-1 DRITIEMAVAPIALALSAHPG--LEEHDLSSLRYIMWCATPVTQTVAEAV
TH_4258|M.thermoresistible__bu DRITIEMAVAPIALALAGHPG--LESYDLSSLRYIMWCATPVTQSVADAV
MMAR_4793|M.marinum_M DRITVEMAVAPIALAMASHPN--LESYDLSSLRFIMWGATPVSAGIAETV
MUL_0362|M.ulcerans_Agy99 DRITVEMAVAPIALAMASHPN--LESYDLSSLRFIMWGATPVSAGIAETV
MAV_0863|M.avium_104 DRVTVEMAVAPIALAIASHPD--LESYDLSSLRYIVWGATPVNAEVAQTV
MSMEG_4876|M.smegmatis_MC2_155 DGITIEMAVAPIALALAAHPG--LERFDLSSLRFVMWCATPVTRSVAEDI
MAB_4055c|M.abscessus_ATCC_199 YRVDHVFVAPPVAVVLAKHPD--VDKYDVSCLRSVFSGAAPLDEQLGNAV
MLBr_02546|M.leprae_Br4923 HQATAMVVVPVMLSRILDTLENLETKSDLSSLRVVFVSGSQLGAELATRA
. : : *:*.** . .: : :
Mb3591|M.bovis_AF2122/97 QSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRIA
Rv3561|M.tuberculosis_H37Rv QSELDIDIVLTAYGLTEANGMGTMCRPEDDAVTVATTCGRPFADFELRIA
Mflv_2392|M.gilvum_PYR-GCK TARTGVTWVT-AYGASELP-VISCNDLQHAR---LDTVGRAVTGVGIRIV
Mvan_4256|M.vanbaalenii_PYR-1 SARTGATWVT-AYGASELP-VISCNDLQDAR---LDTVGRAVAGVDIRIV
TH_4258|M.thermoresistible__bu TARTGVGWIA-AYGTTELP-VIACNPLQGGR---LDTVGRPVPGVQVRIV
MMAR_4793|M.marinum_M TNRTGIGWVP-AYGTTELP-VIACSPLRGAR---LDTVGRVLPGVDLRVV
MUL_0362|M.ulcerans_Agy99 TNRTGIGWVP-AYGTTELP-VIACSPLRGAR---LDTVGRVLPGVDLRVV
MAV_0863|M.avium_104 TRRTGVGWLP-AYGTTELP-VIACNPIEDAR---LDTVGRPVPGVEVRVV
MSMEG_4876|M.smegmatis_MC2_155 TARAGIRWLT-AYGTTELP-VIACNPLDDTR---IETVGKPVRGVRVRIA
MAB_4055c|M.abscessus_ATCC_199 AARLNCRVSQ-GYGMTELS-PVSHLIPPDRPDIPLNSVGIPVPNSENKII
MLBr_02546|M.leprae_Br4923 LEELGPVIYN-MYGSTEIAFATIAGPKDLQIN--SATVGPVVKGVKVKIL
. . ** :* : * . . ::
Mb3591|M.bovis_AF2122/97 D----------------DGEVLLRGPNVMVGYLDDTEATAAAIDADGWLH
Rv3561|M.tuberculosis_H37Rv D----------------DGEVLLRGPNVMVGYLDDTEATAAAIDADGWLH
Mflv_2392|M.gilvum_PYR-GCK SLQTGEVLGA-----GEEGEIQVRSDSAMAGYLPDQWTAQAFS--DGWYR
Mvan_4256|M.vanbaalenii_PYR-1 SLESGAPLGP-----GDEGEIQVRSDSAMAGYLPDHWTADAFC--DGWYR
TH_4258|M.thermoresistible__bu SLDTGAPVGP-----GEEGEVQARSASVMAGYLPDQ--PGAFV--EGWYR
MMAR_4793|M.marinum_M SIETGERVGP-----GEVGEIQARSHSLMAGYLPAAATAEVMS--QGWYR
MUL_0362|M.ulcerans_Agy99 STETGERVGP-----GEVGEIQARSHLLMAGYLPAAATAEVMS--QGWYR
MAV_0863|M.avium_104 SLESGEPAGA-----GEVGEIQARSASLMAGYLPGEATGEAIR--DGWYR
MSMEG_4876|M.smegmatis_MC2_155 ----------------DTGEIQVCSPSAMCGYLPESATGGAFD--DGWYR
MAB_4055c|M.abscessus_ATCC_199 DTETGDEIEIPAEGESAPGELLVRGPNVMAGYLGNEEATAATIEPDGFLH
MLBr_02546|M.leprae_Br4923 DDSGKEVPRG------QVGRIFVGNAFPFEGYTGGGGKQIIDG----LLS
*.: . : **
Mb3591|M.bovis_AF2122/97 TGDIGAVDQAGNLRINDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAV
Rv3561|M.tuberculosis_H37Rv TGDIGAVDQAGNLRITDRLKDMYICGGFNVYPAEVEQVLARMDGVADAAV
Mflv_2392|M.gilvum_PYR-GCK TGDVGTLDGEGWLRITDRAKEMIKVRGFQVAPAEVEAVLHGHPAVEDCAV
Mvan_4256|M.vanbaalenii_PYR-1 TGDVGSLDADGWLRITDRAKEMIKVRGFQVAPAEVEAVLHGHPAVEDCAV
TH_4258|M.thermoresistible__bu TGDVGYLDTDGWLRLTDRAKDMVKVRGFQVAPAEVEAVLHEHPAVDDCAV
MMAR_4793|M.marinum_M TGDIGSLDAAGWLRVTDRLKEMIKVRGFQVAPAEIEAVLHGHPAVRDCAV
MUL_0362|M.ulcerans_Agy99 TGDIGSLDAAGWLRVTDRLKEMIKVRGFQVAPTEIEAVLHGHPAVRDCAV
MAV_0863|M.avium_104 TGDAGWLDTGGWLRITDRLKEMIKVRGFQVAPAEIDAVLQGHPAVADCAV
MSMEG_4876|M.smegmatis_MC2_155 TGDIGFLDDDGYLHVTDRAKEMVKVRGFQVAPAEVEAVLHGHPAVADCAV
MAB_4055c|M.abscessus_ATCC_199 TGDIAVVRADGVVTIVDRLKELIKYKGYQVPPAELEALLLTHPGIGDAAV
MLBr_02546|M.leprae_Br4923 SGDVGYFDEHDLLYISGRDDEMIVSGGENVFPAEVEDLISGHPEVVEATA
:** . . . : : .* .:: * :* *:*:: :: : :.:.
Mb3591|M.bovis_AF2122/97 IGVPDQRLGEVGRAFVVARPGTGLDEASVIAYTREHLANFKTPRSVRFVD
Rv3561|M.tuberculosis_H37Rv IGVPDQRLGEVGRAFVVARPGTGLDEASVIAYTREHLANFKTPRSVRFVD
Mflv_2392|M.gilvum_PYR-GCK FGIPA-ADGEAIVAAVTLGHP--VDTDELADLVADRLASYKRPSRVVVVD
Mvan_4256|M.vanbaalenii_PYR-1 FGVPA-ADGEAIVAAVAASGP--LDTDDLVALVAERLAGYKRLSQVFVVD
TH_4258|M.thermoresistible__bu FGVPS-PDGEALVAAVTRRGE--VSADELLALVGERLASYKRPSRVVFVP
MMAR_4793|M.marinum_M FGVPDGANGEAVVAAVAARTP--VGDAELRARVAASLASYKRLSRIVFVP
MUL_0362|M.ulcerans_Agy99 FGVPDGANGEAVVVAVAARTP--VDAAELRARVAASLASYKRLSRIVFVP
MAV_0863|M.avium_104 FGVPDGLNGEAVVAAVAVSAP--VDVTELTALVRQKLASYKHLSRVVVVP
MSMEG_4876|M.smegmatis_MC2_155 FGEPHDTDGEAVVAAVATCAE--VSAEELIDLVGARLASYKRPRRIEFVT
MAB_4055c|M.abscessus_ATCC_199 IGVPDPSSGEIPKAFVVRTDDD-LTDEAVMAFVEQKVAPHKRIRQVEFID
MLBr_02546|M.leprae_Br4923 IGVDDKEWGARLRAFVVKKQDATIDEDAIKAYVRDHLARYKVPREVIFLE
:* * . *. : : . :* .* : .:
Mb3591|M.bovis_AF2122/97 VLPRNAAGKVSKPQLRELG-----------------
Rv3561|M.tuberculosis_H37Rv VLPRNAAGKVSKPQLRELG-----------------
Mflv_2392|M.gilvum_PYR-GCK EIPRLPSGKVLRRVLRERAMGAGAKSGGF-------
Mvan_4256|M.vanbaalenii_PYR-1 EIPRLPSGKVLRRVLRERAMSARAESGGFGGRPSDQ
TH_4258|M.thermoresistible__bu EIPRLPSGKVLRRVLKER---HGCPSDG--------
MMAR_4793|M.marinum_M DIPRLPSGKVLRRALKEL---YGCASDS--------
MUL_0362|M.ulcerans_Agy99 DIPRLPSGKVLRRALKEL---YGCTSDS--------
MAV_0863|M.avium_104 EIPRLPSGKVLRRVLKER---HGCTSDS--------
MSMEG_4876|M.smegmatis_MC2_155 EIPRLPSGKVLRRVLKER---HGRPSDG--------
MAB_4055c|M.abscessus_ATCC_199 AIPKSAAGKILRKDLRARI-----------------
MLBr_02546|M.leprae_Br4923 ELPRNPTGKVLKRELRDFEVH---------------
:*: .:**: : *: