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M. smegmatis MC2 155 MSMEG_5354 (-)

annotation: O-methyltransferase, family protein 2
coordinates: 5436911 - 5437936
length: 341

MATRSIPAALPDYERMMAMLTGFWVTQIVRAAAVFNLADHLASGTDTAVAIASAEATDVDATRRLLRACA
TLGLVTSADGEHFAGTSLLSTLRKDEPNSLRGMVLSHTAPGHWLPWGLLPDAIRSGERQIKAAHGTETYF
DYLAENLEQAGHFTEAMSNLSAAAAVEVAEVLDTRGVDYALDVGGANGELVRALMRANPELRGGVFDLPH
VVADAVEAAHRDGLQGRFTARGGDFFESVPPADLYTLKYILHDWDDESCVRILKTCRAALQDGGRIVVID
HLVGDLDAADTTTMMDVNMLVMTGGRERDIAEFDALFAAAGLRRTEVGRAGQFAIIETVAV

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. smegmatis MC2 155 MSMEG_5354 - - 100% (341) O-methyltransferase, family protein 2
M. smegmatis MC2 155 MSMEG_2213 - 1e-52 37.01% (335) O-methyltransferase
M. smegmatis MC2 155 MSMEG_0367 - 1e-47 34.63% (335) O-demethylpuromycin-O-methyltransferase

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb0582 - 5e-20 26.88% (320) methyltransferase/methylase
M. gilvum PYR-GCK Mflv_3940 - 2e-49 34.83% (333) O-methyltransferase family protein
M. tuberculosis H37Rv Rv0567 - 1e-20 27.19% (320) methyltransferase/methylase
M. leprae Br4923 - - - - -
M. abscessus ATCC 19977 - - - - -
M. marinum M MMAR_0934 - 3e-22 27.73% (321) methyltransferase/methylase
M. avium 104 - - - - -
M. thermoresistible (build 8) TH_3167 - 8e-45 33.44% (326) PUTATIVE hydroxyneurosporene-O-methyltransferase
M. ulcerans Agy99 MUL_0686 - 7e-22 27.41% (321) methyltransferase/methylase
M. vanbaalenii PYR-1 Mvan_2460 - 2e-44 32.93% (331) O-methyltransferase family protein

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_3940|M.gilvum_PYR-GCK          -----------------MTHTSTPRGSAARSAPNRVPPVRLIRAVDRLRA
Mvan_2460|M.vanbaalenii_PYR-1       ----------------------------MRTMLMTPPPVVLA-AVGRFRG
TH_3167|M.thermoresistible__bu      LQTEYVEVTRIAPVRSRRYDPITRSGGVVMSARVRVPPARIARTADAARH
MSMEG_5354|M.smegmatis_MC2_155      --------------------------------------------------
Mb0582|M.bovis_AF2122/97            --------------------------------------------------
Rv0567|M.tuberculosis_H37Rv         --------------------------------------------------
MMAR_0934|M.marinum_M               --------------------------------------------------
MUL_0686|M.ulcerans_Agy99           --------------------------------------------------
                                                                                      

Mflv_3940|M.gilvum_PYR-GCK          GLQRLHRSTAPGNVALMELATGAWTTQVLYVAATLGIPDELADGPAHAAD
Mvan_2460|M.vanbaalenii_PYR-1       LLDRLHTLTAPSHIAVLELSLGSWFTAALYATVRLGIADALADGPLRADD
TH_3167|M.thermoresistible__bu      YLLRLQQKLTAPQAVMLEMILAGWTSQALTTAADLGVADALADGPLAIGD
MSMEG_5354|M.smegmatis_MC2_155      MATRSIPAALPDYERMMAMLTGFWVTQIVRAAAVFNLADHLASGTDTAVA
Mb0582|M.bovis_AF2122/97            -------MELS-PDRIMAIGGGYGPSKVLLTAVGLGLFTELGDEAMTAEA
Rv0567|M.tuberculosis_H37Rv         -------MELS-PDRIMAIGGGYGPSKVLLTAVGLGLFTELGDEAMTAEA
MMAR_0934|M.marinum_M               -------MDQSSPDRIMAIGAGYGPSKVLLTAVGLGLFTELGDRAMTAQA
MUL_0686|M.ulcerans_Agy99           -------MDQSRPDRIMAIGAGYGPSKVLLTAVGLGLFTELGDRAMTAQA
                                              .    :: :  .   :  : .:. :.:   *.. .     

Mflv_3940|M.gilvum_PYR-GCK          VATRVGADAGAVYRLMRAMVSRGALREERDG---RFALTPVGDALRTDSE
Mvan_2460|M.vanbaalenii_PYR-1       VARKVGADPEATYRLMRALASRSVLKLRRDG---RFALTRLGHALRVDHP
TH_3167|M.thermoresistible__bu      LARKVDADPERLARLMCALTSRGVFRQRRDG---RYALNALATTLRSDAS
MSMEG_5354|M.smegmatis_MC2_155      IASAEATDVDATRRLLRACATLGLVTSADGE---HFAGTSLLSTLRKDEP
Mb0582|M.bovis_AF2122/97            IADRLGLLKRPAIDFLDALVSLDLLARDGDGPGSHYRNTPETAHFLDEAR
Rv0567|M.tuberculosis_H37Rv         IADRLGLLKRPAIDFLDALVSLDLLARDGDGPGSHYRNTPETAHFLDEAR
MMAR_0934|M.marinum_M               IAELLGLVPRPATDFLDALVSLDLLARDGDGPDARYRNTAETARYLDEAS
MUL_0686|M.ulcerans_Agy99           LAELLGLVPRPATDFLDALVSLDLLARDGDGPDARYRNTAETARYLDEAS
                                    :*            :: * .: . .    .    ::  .        :  

Mflv_3940|M.gilvum_PYR-GCK          GSLRDMVLFIGHPARWADWGSLEYSVRTGEPAADMLRGMP-FFDYLDTDP
Mvan_2460|M.vanbaalenii_PYR-1       ESMAPLIAFVGSRQHWEHWGELLYSVQTGRTAVEKLRGSE-FFEYLDTDP
TH_3167|M.thermoresistible__bu      ASLAGAARFYGSRLHREHWSMLTDSVRTGKPGVPTLRGMD-WWQFSAANP
MSMEG_5354|M.smegmatis_MC2_155      NSLRGMVLSHTAPGHWLPWGLLPDAIRSGERQIKAAHGTETYFDYLAENL
Mb0582|M.bovis_AF2122/97            PTYAGGLLKIWNERNYRFWADLTEALKTGKAQSEVKQTGRPFFEALYADP
Rv0567|M.tuberculosis_H37Rv         PTYAGGLLKIWNERNYRFWADLTEALKTGKAQSEVKQTGRPFFEALYADP
MMAR_0934|M.marinum_M               PEYQGGLLKIWDQRNYRFWADLTDALQTGKPQNEIKQTGRPFFETVYADP
MUL_0686|M.ulcerans_Agy99           PEYQGGLLKIWDQRNYRFWADLTDALQTGKPQNEIKQTGRPFFETVYADP
                                                  .   *. *  ::::*.      :    :::    : 

Mflv_3940|M.gilvum_PYR-GCK          EFATVFNNAMTAASGLSDDVALQACDFTGARLVVDVGGGHGAVLATILRS
Mvan_2460|M.vanbaalenii_PYR-1       AFAKVFNESMTGGSRAVIENAIPAYDFSDRRLIVDVGGGEGGLLAAILNR
TH_3167|M.thermoresistible__bu      EFGDLFNGAMTSISELSAPVVAAAYDFSPFPTIVDVGGGHGRLLSAILAA
MSMEG_5354|M.smegmatis_MC2_155      EQAGHFTEAMSNLSAAAAVEVAEVLDTRGVDYALDVGGANGELVRALMRA
Mb0582|M.bovis_AF2122/97            RRLEAFMAAMDAASRRNIELLAKRFPFERYRRLCDVGCADGLLSRIVAAA
Rv0567|M.tuberculosis_H37Rv         RRLEAFMAAMDAASRRNIELLAKRFPFERYRRLCDVGCADGLLSRIVAAA
MMAR_0934|M.marinum_M               RRLEVFLGAMDSASRRNCELLVKRFPFQRYGSLCDVGGANGLLSRTAAAA
MUL_0686|M.ulcerans_Agy99           RRLEVFLGAMDSASRRNCELLVKRFPFQRYGSLCDVGGANGLLSRTAAAA
                                         *  :*   *                    *** ..* :       

Mflv_3940|M.gilvum_PYR-GCK          AAGARGILFDLPAVVAGAAPLLADAGVADRCTVSGGSFLESVPAGGDVYV
Mvan_2460|M.vanbaalenii_PYR-1       TPTARGVLFDRPSVVAGADSVLGPAGVAARCRTEGGSFFEAVPAGGDAYV
TH_3167|M.thermoresistible__bu      TPTARGVLFDLPDVVADAPALLRAHGVADRVDIVGGSFFDAVPDG-DAYV
MSMEG_5354|M.smegmatis_MC2_155      NPELRGGVFDLPHVVADAVEAAHRDGLQGRFTARGGDFFESVPPA-DLYT
Mb0582|M.bovis_AF2122/97            HPHLQCVSLDLPAVTEIARRKLTAEGLGERVQACAGDFLADPLPAADVIT
Rv0567|M.tuberculosis_H37Rv         HPHLQCVSFDLPAVTEIARRKLTAEGLGERVQACAGDFLADPLPAADVIT
MMAR_0934|M.marinum_M               HPHLQCISFDLPAVTDITRRKLSEQGLGDRVRAVSGDFFADPLPSADVIT
MUL_0686|M.ulcerans_Agy99           HPHLQCISFDLPAVTDITRRKLSEQGLGDRVRAVSGDFFADPLPSADVIT
                                     .  :   :* * *.  :       *:  *    .*.*:     . *  .

Mflv_3940|M.gilvum_PYR-GCK          MKNIVHDWDDTDAATILRNVRTAIAEGGKLVLLEMVLPERASSFIG-HML
Mvan_2460|M.vanbaalenii_PYR-1       MKAIIHDWGDDQSLSILRNVRTAIADDGRLLLFEMVLPERAPAHLG-FMV
TH_3167|M.thermoresistible__bu      LKNVIHDWDDDEALPILGNIRSALSDRGAILLVEMVIPSHHREFLG-NMV
MSMEG_5354|M.smegmatis_MC2_155      LKYILHDWDDESCVRILKTCRAALQDGGRIVVIDHLVGDLDAADTT-TMM
Mb0582|M.bovis_AF2122/97            MGQILHDWNLDRKQQLVAKAYEALSKEGAFIVIETLIDDARRENTTGLMM
Rv0567|M.tuberculosis_H37Rv         MGQILHDWNLDRKQQLVAKAYEALSKEGAFIVIETLIDDARRENTTGLMM
MMAR_0934|M.marinum_M               MGQILHDWNLDGKQRLIAKAYEALPEGGAFIAIETLIDDARRKNTAGLMM
MUL_0686|M.ulcerans_Agy99           MGQILHDWNLDGKQRLIAKAYEALPEGGAFTAIETLIDDARRKNTAGLMM
                                    :  ::***.      :: .   *: . * :  .: :: .         *:

Mflv_3940|M.gilvum_PYR-GCK          DLEMLLML-RGKERTRAQYSELLEAAGFQLTRVIPTVSPISVIEARAV
Mvan_2460|M.vanbaalenii_PYR-1       DLEMLVTA-GGRERTASQYAKLLADSGFRMTRVIPTASPLSIVEAVPA
TH_3167|M.thermoresistible__bu      DLEMLNGG-NARERTADEYRSLLARANLRMTRVVATAGPYSVIEARAA
MSMEG_5354|M.smegmatis_MC2_155      DVNMLVMT-GGRERDIAEFDALFAAAGLRRTEVG-RAGQFAIIETVAV
Mb0582|M.bovis_AF2122/97            SLNMLIEFGDAFDYSAADFRGWCGEAGFRSFEVIPLAGGSSAAVAYK-
Rv0567|M.tuberculosis_H37Rv         SLNMLIEFGDAFDYSAADFRGWCGEAGFRSFEVIPLAGGSSAAVAYK-
MMAR_0934|M.marinum_M               SLNMLIEFGDAFDYTGADFRKWCGAAGFRSFEVIALAAGSSAAVAYK-
MUL_0686|M.ulcerans_Agy99           SLNMLIEFGDAFDYTGADFRKWCGAAGFRSFEVIALAAGSSAAVAYK-
                                    .::**     . :    ::      :.::  .*   ..  :   :

species	id	gene	e-value	identity (len)	annotation
M. smegmatis MC2 155	MSMEG_5354	-	-	100% (341)	O-methyltransferase, family protein 2
M. smegmatis MC2 155	MSMEG_2213	-	1e-52	37.01% (335)	O-methyltransferase
M. smegmatis MC2 155	MSMEG_0367	-	1e-47	34.63% (335)	O-demethylpuromycin-O-methyltransferase