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VRILRLRLQDGFLLPSPVLAKTLRRAPVEVSGAQADLRIGHAKGGKVTAFVSEDPTDDDEEALDARERMA RLIFAPRILAQVVKCVAQFGVADHLATGPCHPADLAAVIGLDASAAGRLLRYCTTVGLVRRDKDGRYHGT ELLATLRSDDPYRLRSFAMAQNGPGQWAVLSRLDEALRTGAPQAAEALGCELYEYYGRPDVADEAAAYRQ GLLGRSVDVQDAVVDFVDTRGLASVLDVGGSAGSMVMALMLANPDLRGAVLDLPDAAPAAEAQARRLGVS ERFEFIAGNFFEEVPGSDLFLLKNVLGNWDDDRCVAILENSRRKALPESRWVIVENAVDESSPKRWAVDV DIVTLVAVGGRVRSVDDYRRLLTRAGLRFVRSASATAGYQLIEAVLR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2213 | - | - | 100% (397) | O-methyltransferase |
| M. smegmatis MC2 155 | MSMEG_5354 | - | 1e-52 | 37.01% (335) | O-methyltransferase, family protein 2 |
| M. smegmatis MC2 155 | MSMEG_0367 | - | 8e-40 | 32.30% (322) | O-demethylpuromycin-O-methyltransferase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0582 | - | 6e-09 | 25.00% (172) | methyltransferase/methylase |
| M. gilvum PYR-GCK | Mflv_3940 | - | 8e-38 | 29.84% (315) | O-methyltransferase family protein |
| M. tuberculosis H37Rv | Rv0567 | - | 2e-08 | 24.42% (172) | methyltransferase/methylase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | - | - | - | - | - |
| M. marinum M | MMAR_0934 | - | 1e-09 | 22.53% (293) | methyltransferase/methylase |
| M. avium 104 | - | - | - | - | - |
| M. thermoresistible (build 8) | TH_3167 | - | 2e-40 | 32.91% (316) | PUTATIVE hydroxyneurosporene-O-methyltransferase |
| M. ulcerans Agy99 | MUL_0686 | - | 1e-09 | 22.87% (293) | methyltransferase/methylase |
| M. vanbaalenii PYR-1 | Mvan_2460 | - | 7e-36 | 28.80% (309) | O-methyltransferase family protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_3940|M.gilvum_PYR-GCK -----------------MTHTSTPRGSAARSAPNRVPPVRLIRAVDRLRA
Mvan_2460|M.vanbaalenii_PYR-1 ----------------------------MRTMLMTPPPVVLA-AVGRFRG
TH_3167|M.thermoresistible__bu LQTEYVEVTRIAPVRSRRYDPITRSGGVVMSARVRVPPARIARTADAARH
Mb0582|M.bovis_AF2122/97 --------------------------------------------------
Rv0567|M.tuberculosis_H37Rv --------------------------------------------------
MMAR_0934|M.marinum_M --------------------------------------------------
MUL_0686|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2213|M.smegmatis_MC2_155 ------MRILRLRLQDGFLLPSPVLAKTLRRAPVEVSGAQADLRIGHAKG
Mflv_3940|M.gilvum_PYR-GCK GLQRLHRSTAPGNVALMELATGAWTT----------QVLYVAATLGIPDE
Mvan_2460|M.vanbaalenii_PYR-1 LLDRLHTLTAPSHIAVLELSLGSWFT----------AALYATVRLGIADA
TH_3167|M.thermoresistible__bu YLLRLQQKLTAPQAVMLEMILAGWTS----------QALTTAADLGVADA
Mb0582|M.bovis_AF2122/97 -------MELS-PDRIMAIGGGYGPS----------KVLLTAVGLGLFTE
Rv0567|M.tuberculosis_H37Rv -------MELS-PDRIMAIGGGYGPS----------KVLLTAVGLGLFTE
MMAR_0934|M.marinum_M -------MDQSSPDRIMAIGAGYGPS----------KVLLTAVGLGLFTE
MUL_0686|M.ulcerans_Agy99 -------MDQSRPDRIMAIGAGYGPS----------KVLLTAVGLGLFTE
MSMEG_2213|M.smegmatis_MC2_155 GKVTAFVSEDPTDDDEEALDARERMARLIFAPRILAQVVKCVAQFGVADH
. : : .: .. :*:
Mflv_3940|M.gilvum_PYR-GCK LADGPAHAADVATRVGADAGAVYRLMRAMVSRGALREERDG---RFALTP
Mvan_2460|M.vanbaalenii_PYR-1 LADGPLRADDVARKVGADPEATYRLMRALASRSVLKLRRDG---RFALTR
TH_3167|M.thermoresistible__bu LADGPLAIGDLARKVDADPERLARLMCALTSRGVFRQRRDG---RYALNA
Mb0582|M.bovis_AF2122/97 LGDEAMTAEAIADRLGLLKRPAIDFLDALVSLDLLARDGDGPGSHYRNTP
Rv0567|M.tuberculosis_H37Rv LGDEAMTAEAIADRLGLLKRPAIDFLDALVSLDLLARDGDGPGSHYRNTP
MMAR_0934|M.marinum_M LGDRAMTAQAIAELLGLVPRPATDFLDALVSLDLLARDGDGPDARYRNTA
MUL_0686|M.ulcerans_Agy99 LGDRAMTAQALAELLGLVPRPATDFLDALVSLDLLARDGDGPDARYRNTA
MSMEG_2213|M.smegmatis_MC2_155 LATGPCHPADLAAVIGLDASAAGRLLRYCTTVGLVRRDKDG---RYHGTE
*. . :* :. :: .: . . ** :: .
Mflv_3940|M.gilvum_PYR-GCK VGDALRTDSEGSLRDMVLFIGHPARWADWGSLEYSVRTGEPAADMLR-GM
Mvan_2460|M.vanbaalenii_PYR-1 LGHALRVDHPESMAPLIAFVGSRQHWEHWGELLYSVQTGRTAVEKLR-GS
TH_3167|M.thermoresistible__bu LATTLRSDASASLAGAARFYGSRLHREHWSMLTDSVRTGKPGVPTLR-GM
Mb0582|M.bovis_AF2122/97 ETAHFLDEARPTYAGGLLKIWNERNYRFWADLTEALKTGKAQSEVKQTGR
Rv0567|M.tuberculosis_H37Rv ETAHFLDEARPTYAGGLLKIWNERNYRFWADLTEALKTGKAQSEVKQTGR
MMAR_0934|M.marinum_M ETARYLDEASPEYQGGLLKIWDQRNYRFWADLTDALQTGKPQNEIKQTGR
MUL_0686|M.ulcerans_Agy99 ETARYLDEASPEYQGGLLKIWDQRNYRFWADLTDALQTGKPQNEIKQTGR
MSMEG_2213|M.smegmatis_MC2_155 LLATLRSDDPYRLRSFAMAQNGPGQWAVLSRLDEALRTGAP-QAAEALGC
: . . * :::** . *
Mflv_3940|M.gilvum_PYR-GCK PFFDYLDTD--PEFATVFNNAMTAASGLSDDVALQACDFTGARLVVDVGG
Mvan_2460|M.vanbaalenii_PYR-1 EFFEYLDTD--PAFAKVFNESMTGGSRAVIENAIPAYDFSDRRLIVDVGG
TH_3167|M.thermoresistible__bu DWWQFSAAN--PEFGDLFNGAMTSISELSAPVVAAAYDFSPFPTIVDVGG
Mb0582|M.bovis_AF2122/97 PFFEALYAD--PRRLEAFMAAMDAASRRNIELLAKRFPFERYRRLCDVGC
Rv0567|M.tuberculosis_H37Rv PFFEALYAD--PRRLEAFMAAMDAASRRNIELLAKRFPFERYRRLCDVGC
MMAR_0934|M.marinum_M PFFETVYAD--PRRLEVFLGAMDSASRRNCELLVKRFPFQRYGSLCDVGG
MUL_0686|M.ulcerans_Agy99 PFFETVYAD--PRRLEVFLGAMDSASRRNCELLVKRFPFQRYGSLCDVGG
MSMEG_2213|M.smegmatis_MC2_155 ELYEYYGRPDVADEAAAYRQGLLGRSVDVQDAVVDFVDTRGLASVLDVGG
:: . : .: . * : ***
Mflv_3940|M.gilvum_PYR-GCK GHGAVLATILRSAAGARGILFDLPAVVAGAAPLLADAGVADRCTVSGGSF
Mvan_2460|M.vanbaalenii_PYR-1 GEGGLLAAILNRTPTARGVLFDRPSVVAGADSVLGPAGVAARCRTEGGSF
TH_3167|M.thermoresistible__bu GHGRLLSAILAATPTARGVLFDLPDVVADAPALLRAHGVADRVDIVGGSF
Mb0582|M.bovis_AF2122/97 ADGLLSRIVAAAHPHLQCVSLDLPAVTEIARRKLTAEGLGERVQACAGDF
Rv0567|M.tuberculosis_H37Rv ADGLLSRIVAAAHPHLQCVSFDLPAVTEIARRKLTAEGLGERVQACAGDF
MMAR_0934|M.marinum_M ANGLLSRTAAAAHPHLQCISFDLPAVTDITRRKLSEQGLGDRVRAVSGDF
MUL_0686|M.ulcerans_Agy99 ANGLLSRTAAAAHPHLQCISFDLPAVTDITRRKLSEQGLGDRVRAVSGDF
MSMEG_2213|M.smegmatis_MC2_155 SAGSMVMALMLANPDLRGAVLDLPDAAPAAEAQARRLGVSERFEFIAGNF
. * : . : :* * .. : *:. * .*.*
Mflv_3940|M.gilvum_PYR-GCK LESVPAGGDVYVMKNIVHDWDDTDAATILRNVRTAIAEGGKLVLLEMVLP
Mvan_2460|M.vanbaalenii_PYR-1 FEAVPAGGDAYVMKAIIHDWGDDQSLSILRNVRTAIADDGRLLLFEMVLP
TH_3167|M.thermoresistible__bu FDAVPDG-DAYVLKNVIHDWDDDEALPILGNIRSALSDRGAILLVEMVIP
Mb0582|M.bovis_AF2122/97 LADPLPAADVITMGQILHDWNLDRKQQLVAKAYEALSKEGAFIVIETLID
Rv0567|M.tuberculosis_H37Rv LADPLPAADVITMGQILHDWNLDRKQQLVAKAYEALSKEGAFIVIETLID
MMAR_0934|M.marinum_M FADPLPSADVITMGQILHDWNLDGKQRLIAKAYEALPEGGAFIAIETLID
MUL_0686|M.ulcerans_Agy99 FADPLPSADVITMGQILHDWNLDGKQRLIAKAYEALPEGGAFTAIETLID
MSMEG_2213|M.smegmatis_MC2_155 FEEVPGS-DLFLLKNVLGNWDDDRCVAILENSRRKALPESRWVIVENAVD
: . * : :: :*. :: : . .* :
Mflv_3940|M.gilvum_PYR-GCK ERAS-SFIGHMLDLEMLLML-RGKERTRAQYSELLEAAGFQLTRVIPTVS
Mvan_2460|M.vanbaalenii_PYR-1 ERAP-AHLGFMVDLEMLVTA-GGRERTASQYAKLLADSGFRMTRVIPTAS
TH_3167|M.thermoresistible__bu SHHR-EFLGNMVDLEMLNGG-NARERTADEYRSLLARANLRMTRVVATAG
Mb0582|M.bovis_AF2122/97 DARRENTTGLMMSLNMLIEFGDAFDYSAADFRGWCGEAGFRSFEVIPLAG
Rv0567|M.tuberculosis_H37Rv DARRENTTGLMMSLNMLIEFGDAFDYSAADFRGWCGEAGFRSFEVIPLAG
MMAR_0934|M.marinum_M DARRKNTAGLMMSLNMLIEFGDAFDYTGADFRKWCGAAGFRSFEVIALAA
MUL_0686|M.ulcerans_Agy99 DARRKNTAGLMMSLNMLIEFGDAFDYTGADFRKWCGAAGFRSFEVIALAA
MSMEG_2213|M.smegmatis_MC2_155 ESSPKRWAVDVDIVTLVAVG--GRVRSVDDYRRLLTRAGLRFVRSASATA
. : : :: . : :: :.:: . . ..
Mflv_3940|M.gilvum_PYR-GCK PISVIEARAV
Mvan_2460|M.vanbaalenii_PYR-1 PLSIVEAVPA
TH_3167|M.thermoresistible__bu PYSVIEARAA
Mb0582|M.bovis_AF2122/97 GSSAAVAYK-
Rv0567|M.tuberculosis_H37Rv GSSAAVAYK-
MMAR_0934|M.marinum_M GSSAAVAYK-
MUL_0686|M.ulcerans_Agy99 GSSAAVAYK-
MSMEG_2213|M.smegmatis_MC2_155 GYQLIEAVLR
. *