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QIAEFSALSDLELEGQARGWAQAEASASAHKHAAMAEMFIRATSTPSADERRWWFVDPDAAVGAQLGAAQ GITAWAALHQAQRGVALRDRLPKVNEVFAAGLVSEMIVRNICWSTALMLEPDKLALIDAELAAQISGWGK LTLKEIDNTIDDLILKHDPASFRRGRASRRGRYFDIGSPTDAPGVLTVTGRLQAHLGNAYDARIAELIEG VCPDDPRTLNELRHDALGAILDQTTLACECEDPDCVRGREQSSRGHLVVHVIAREDTVTAAQHAATTTNT PDDAPSDTPAADTEPAEPAEPAEPVDDPDIAPAATATETATDTEPDAPAPTDTADSAATETDCDPESVSV TEFTDNSSDTPAAFLDPDEHPLDRVPPAVLFGGGVLPAYALAEIINNATIRPLKHPGDTAPEDRYIPSRA LADYVRCRDLTCRFPGCDKPADRCDLDHTVPYPAGPTHASNLKCLCRFHHLLKTFWTGPRGWTDRQHPDG TIVWTSPSGRQYTTVPGSHRRLSITELAAPTGALDLPATPIPTADPDLRGVKMPKRRRTRAQNTARTIAA ERQLNDDLVAEHNKPPPF*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5106 | - | - | 100% (579) | HNH endonuclease |
| M. smegmatis MC2 155 | MSMEG_0144 | - | 0.0 | 92.96% (597) | HNH endonuclease |
| M. smegmatis MC2 155 | MSMEG_5583 | - | 2e-95 | 36.28% (623) | HNH endonuclease |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2126 | - | 6e-99 | 40.46% (561) | hypothetical protein Mb2126 |
| M. gilvum PYR-GCK | Mflv_1180 | - | 1e-114 | 42.64% (584) | hypothetical protein Mflv_1180 |
| M. tuberculosis H37Rv | Rv2100 | - | 6e-99 | 40.46% (561) | hypothetical protein Rv2100 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4752 | - | 3e-28 | 48.87% (133) | hypothetical protein MAB_4752 |
| M. marinum M | MMAR_5187 | - | 1e-53 | 54.64% (183) | 13e12 repeat-containing protein |
| M. avium 104 | MAV_4456 | - | 4e-55 | 53.88% (206) | 13e12 repeat-containing protein |
| M. thermoresistible (build 8) | TH_0334 | - | 2e-29 | 31.95% (266) | HNH endonuclease |
| M. ulcerans Agy99 | MUL_1813 | - | 3e-42 | 45.50% (222) | 13e12 repeat-containing protein |
| M. vanbaalenii PYR-1 | Mvan_0728 | - | 1e-118 | 43.55% (581) | hypothetical protein Mvan_0728 |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_5187|M.marinum_M ------------------------MFESMGVAAGVDHPCDALAERRQLRT
MAV_4456|M.avium_104 ------------------------------MCEDATAWFDELMER-CYPS
MUL_1813|M.ulcerans_Agy99 --------------------------------------------------
MAB_4752|M.abscessus_ATCC_1997 ------------------------------------------------MF
TH_0334|M.thermoresistible__bu ------------------------------------------------MF
Mflv_1180|M.gilvum_PYR-GCK --------------------------------------MFDNLVPEPAAL
Mvan_0728|M.vanbaalenii_PYR-1 --------------------------------------MFDVLVPDPADL
MSMEG_5106|M.smegmatis_MC2_155 --------------------------------------------MQIAEF
Mb2126|M.bovis_AF2122/97 MAGALFEPSFAAAHPAGLLRRPVTRTVVLSVAATSIAHMFEISLPDPTEL
Rv2100|M.tuberculosis_H37Rv MAGALFEPSFAAAHPAGLLRRPVTRTVVLSVAATSIAHMFEISLPDPTEL
MMAR_5187|M.marinum_M PTAESARWVQRIGAAARAENRAAAAQLIEIGQLFAYR--LSRCSETEDWA
MAV_4456|M.avium_104 ATPESAALVQRIGVFARVENRAAAEQLAAIGELFAYR--LSRCSECEDWA
MUL_1813|M.ulcerans_Agy99 --------MERAREASRREAAAAAERLDVVGALFVLR--CRESGDRAEWA
MAB_4752|M.abscessus_ATCC_1997 DELGDVELIDLIASAQRAEAQACARKLDAIGVLVDRHADLGKPDERDHHA
TH_0334|M.thermoresistible__bu ESLDDGAVVDSIAAASREQSAACGREMLAIGELYARR-APEDDTERANWA
Mflv_1180|M.gilvum_PYR-GCK AGLSDAELVDAAGAAARAENAVCARKLAVMAELFTRRVDLAPED-RLNWW
Mvan_0728|M.vanbaalenii_PYR-1 AALSDAELLDAATATTRAENAVCARKLAVMAELFIRRTTLAPQD-RLDWW
MSMEG_5106|M.smegmatis_MC2_155 SALSDLELEGQARGWAQAEASASAHKHAAMAEMFIRATSTPSADERRWWF
Mb2126|M.bovis_AF2122/97 CRSDDGALVAAIEDCARVEAAASARRLSAIAELTGRRTG---ADQRADWA
Rv2100|M.tuberculosis_H37Rv CRSDDGALVAAIEDCARVEAAASARRLSAIAELTGRRTG---ADQRADWA
: : ... . :. :
MMAR_5187|M.marinum_M VDTMEAVAAEVAAGLRISQGLAASRLRYARALRERLPKTGAVFAAGQIDL
MAV_4456|M.avium_104 VDTEAAVSAEVAAQLRIGQGLAAAKVRYARAMRERLPRVGEVFRAGEIDF
MUL_1813|M.ulcerans_Agy99 TDTWEAVGAQVAAALRTSVAMGSSYLRYAMAMRNRLPKVGQAFRAEDIDY
MAB_4752|M.abscessus_ATCC_1997 LDRWDRVASEVAAAQGITHALASSQMRYAQVLRHRLRAVAERFAAGDLDF
TH_0334|M.thermoresistible__bu IDGHANVVAEISAALNISRGRAAGRLQLAIDLRERLPRVAAVFAAGDIDF
Mflv_1180|M.gilvum_PYR-GCK VDPDAAVAAEIAAAVTITQGLATHQTYRAVVLRDRLPKVGALFLAGLLSE
Mvan_0728|M.vanbaalenii_PYR-1 VDPEAAVTAELAAAHHITQGLATHQAYRAVVLRDRLPKIGALFLQGLLSE
MSMEG_5106|M.smegmatis_MC2_155 VDPDAAVGAQLGAAQGITAWAALHQAQRGVALRDRLPKVNEVFAAGLVSE
Mb2126|M.bovis_AF2122/97 CDFWDCAAAEVAAALTISHGKASGQMHLSLALN-RLPQVAALFLAGHLGA
Rv2100|M.tuberculosis_H37Rv CDFWDCAAAEVAAALTISHGKASGQMHLSLALN-RLPQVAALFLAGHLGA
* . :::.* . . :. ** * :.
MMAR_5187|M.marinum_M RAFQTIVFRTDLIVDPQVMAAVDALVALNVGRWPSMTKARLSGQIDAIVA
MAV_4456|M.avium_104 RLFATIVYRTDLITDRRVLAAVDAELAATAARWPSMTRARLAARVDAVVA
MUL_1813|M.ulcerans_Agy99 RAFQTIVFRTDLITDAEVLAKVDAQLAALLARCPAVTRGRLSVAVDQVVA
MAB_4752|M.abscessus_ATCC_1997 RTVALIINRTELVDDEAVVARVDAAIVRALPRWARWSHKRITAALDALVL
TH_0334|M.thermoresistible__bu RMVSALVNRSRNVVDPDLLARLDAALAAWSPKWMKLSGPTLNERIDMWVE
Mflv_1180|M.gilvum_PYR-GCK LLVRAIITRTDLITDPELIAAVDADLAADILRWGPQSAKKTEAAIDHIVE
Mvan_0728|M.vanbaalenii_PYR-1 LLVRAIITRTGLITDPDLIAAVDADLATDILDWGPLSVKATEAEIDAIID
MSMEG_5106|M.smegmatis_MC2_155 MIVRNICWSTALMLEPDKLALIDAELAAQISGWGKLTLKEIDNTIDDLIL
Mb2126|M.bovis_AF2122/97 RLFSIIAWRTYLVRDPHALSLLDAALAEHAGAWGPLSAPKLEKAIDSWID
Rv2100|M.tuberculosis_H37Rv RLFSIIAWRTYLVRDPHALSLLDAALAEHAGAWGPLSAPKLEKAIDSWID
. : : : : :: :** :. : :* :
MMAR_5187|M.marinum_M RADADAVRRRKERHADREIWIGQDADG--ISRIDGSMFTIDAHALDRRLS
MAV_4456|M.avium_104 KADADAVRRTEKQAASRHVWIAEMGEG--LSHLEGTVSSPDAHALDKRLD
MUL_1813|M.ulcerans_Agy99 DVDRDVVRRVAEAADGRFVDVDVIADGSGLAALAGSVLATAGRAFDRRLD
MAB_4752|M.abscessus_ATCC_1997 HFDQDAVRRNRSATDKRHLEIRPLGNGEPVAEVWGTLQAVHAVALDKRLD
TH_0334|M.thermoresistible__bu RFDPTGVREPRSPGEDRYVVVTPAGQG--MVDVSARLTFEAGFAFXTRLD
Mflv_1180|M.gilvum_PYR-GCK RHDPDAVRQSRAGDLDRAIEIGNPGDAPGYTTVWARMFAGDAAAGQRTLT
Mvan_0728|M.vanbaalenii_PYR-1 RHDPDAVRKSRAPETGRTVQFGNPGDAPGYTSMWARMYASDAAAADRTLT
MSMEG_5106|M.smegmatis_MC2_155 KHDPASFRRGRASRRGRYFDIGSPTDAPGVLTVTGRLQAHLGNAYDARIA
Mb2126|M.bovis_AF2122/97 RYDPGALRRSRISARTRDLCIGDPDEDAGTAALWGRLYATDAAMLDRRLT
Rv2100|M.tuberculosis_H37Rv RYDPGALRRSRISARTRDLCIGDPDEDAGTAALWGRLYATDAAMLDRRLT
* .*. * . . : : . : . :
MMAR_5187|M.marinum_M ALAATVCAQDPRSREQRRADALGVLAAGGDRLGCRCGLPDC-AAGARPAA
MAV_4456|M.avium_104 ALAATVCEHDPRTGEQRRADALGALAAGADRLGCRCGRADC-AAGTRPAA
MUL_1813|M.ulcerans_Agy99 ELANTVCAADPRTREQRRADAVGALVAGSDRLACGCTSADC-TAAARSAP
MAB_4752|M.abscessus_ATCC_1997 LLAGTVCPADPRSKTQRRADAVGALTQGLNRMRCACGHPDC-SGRD-TAD
TH_0334|M.thermoresistible__bu QIAATVCRDDPRTADQRRADAMAAMSEGQNRLVCGCGSEDCPADPADTAA
Mflv_1180|M.gilvum_PYR-GCK AMAYSVCDNDPRTLDERRNDALTALFHGITTLACRCGHTDCDAAT-NPRP
Mvan_0728|M.vanbaalenii_PYR-1 ALAYSVCDNDPRTLDERRNDAHTALLTGITTLACQCGNPDCHAST-NPRP
MSMEG_5106|M.smegmatis_MC2_155 ELIEGVCPDDPRTLNELRHDALGAILD-QTTLACECEDPDCVRGR-EQSS
Mb2126|M.bovis_AF2122/97 EMAHGVCEDDPRTLAQRRADALGALAAGADHLACGCGKPDCPSGAGNDER
Rv2100|M.tuberculosis_H37Rv EMAHGVCEDDPRTLAQRRADALGALAAGADHLACGCGKPDCPSGAGNDER
: ** ***: : * ** .: : * * **
MMAR_5187|M.marinum_M QP-VVIHVIAERATLDG---------------------------------
MAV_4456|M.avium_104 GP-VVIHLIAERASVEG---------------------------------
MUL_1813|M.ulcerans_Agy99 SK-VVIHVVADQSTVDG---------------------------------
MAB_4752|M.abscessus_ATCC_1997 VP-VVVNVVINQSTLAG---------------------------------
TH_0334|M.thermoresistible__bu TR-LVINVIAEQATVEG---------------------------------
Mflv_1180|M.gilvum_PYR-GCK PRELTVYALTDQTTADSASEEPRG----DATDADASAGDAQPATPTAPKP
Mvan_0728|M.vanbaalenii_PYR-1 PRDITVYALTDHTTTT------------DTGDADTTT-DTKPPTPPAPQP
MSMEG_5106|M.smegmatis_MC2_155 RGHLVVHVIAREDTVTAAQHAATTTNTPDDAPSDTPAADTEPAEPAEPAE
Mb2126|M.bovis_AF2122/97 AAGVVIHVVADASALDAQPDPHLSG---DEPPSRPLTPETTLFEALTPDP
Rv2100|M.tuberculosis_H37Rv AAGVVIHVVADASALDAQPDPHLSG---DEPPSRPLTPETTLFEALTPDP
:.: : :
MMAR_5187|M.marinum_M DGGAP---------------------------------------------
MAV_4456|M.avium_104 TGGGP---------------------------------------------
MUL_1813|M.ulcerans_Agy99 TGHTP---------------------------------------------
MAB_4752|M.abscessus_ATCC_1997 SDDCP---------------------------------------------
TH_0334|M.thermoresistible__bu RSDNP---------------------------------------------
Mflv_1180|M.gilvum_PYR-GCK RRSKPTLLPGR---------------------------------------
Mvan_0728|M.vanbaalenii_PYR-1 RRSKPILIPGR---------------------------------------
MSMEG_5106|M.smegmatis_MC2_155 PVDDPDIAPAATATETATDTEPDAPAPTDTADSAATETDCDPESVSVTEF
Mb2126|M.bovis_AF2122/97 EPDPPATHAPA---------------------------------------
Rv2100|M.tuberculosis_H37Rv EPDPPATHAPA---------------------------------------
*
MMAR_5187|M.marinum_M ------------------------AWEVGADGLVTPELVAELAKSAKLVP
MAV_4456|M.avium_104 ------------------------AVEVGAEGLLPAQLVAELAASARLMP
MUL_1813|M.ulcerans_Agy99 ------------------------AVLLGAEGLIPASLIAELAKVATLRP
MAB_4752|M.abscessus_ATCC_1997 ------------------------AHVPGFG-----VLAAESARAAVAGT
TH_0334|M.thermoresistible__bu ------------------------GYLPGYG-AVPASILRAKAATATLRP
Mflv_1180|M.gilvum_PYR-GCK -----------------------SGYLFGSGFMSAPLFDALLDG-AKIRE
Mvan_0728|M.vanbaalenii_PYR-1 -----------------------PAYLFGAGFMPAPLFDALLAE-ATIRE
MSMEG_5106|M.smegmatis_MC2_155 TDNSSDTPAAFLDPDEHPLDRVPPAVLFGGGVLPAYALAEIINN-ATIRP
Mb2126|M.bovis_AF2122/97 -----------------------ELITTGGGVVPAPLLAELIRGGATISQ
Rv2100|M.tuberculosis_H37Rv -----------------------ELITTGGGVVPAPLLAELIRGGATISQ
* : *
MMAR_5187|M.marinum_M LRHPG-DVPPEPGYVPSKALADFVRCRDLTCRWPGCDHPALNCDLDHTIP
MAV_4456|M.avium_104 LVHPA-DAPPENRYTPSAALADFVRCRDLTCRWPGCDRPATECDLDHTIP
MUL_1813|M.ulcerans_Agy99 LMRPS-GRP-ESGYAPSAGLAEFVRCRDLTCRARGCDQPAVCCDLDHTVA
MAB_4752|M.abscessus_ATCC_1997 PTRNL-TDSRSTGYRPSAKLAAFVRSRDQLCRFPGCEAVADRADIDHTVP
TH_0334|M.thermoresistible__bu VALP--QPCAEAGYRPSAALTRFIRCRDLTCRFPGCDAPAAVCQIDHTIP
Mflv_1180|M.gilvum_PYR-GCK IIHPG-QAGPEPRYTPSTALADFIRCRDLTCRFPGCDTPATEADIDHTVA
Mvan_0728|M.vanbaalenii_PYR-1 IIHPGNRSAPEPRYTPSQALAEFIRCRDLTCRFPGCDKPATEADIDHTVP
MSMEG_5106|M.smegmatis_MC2_155 LKHPG-DTAPEDRYIPSRALADYVRCRDLTCRFPGCDKPADRCDLDHTVP
Mb2126|M.bovis_AF2122/97 VRHPG-DLAAEPHYRPSAKLAEFVRMRDLTCRFPGCDVPAEFCDIDHSAP
Rv2100|M.tuberculosis_H37Rv VRHPG-DLAAEPHYRPSAKLAEFVRMRDLTCRFPGCDVPAEFCDIDHSAP
. * ** *: ::* ** ** **: * .::**: .
MMAR_5187|M.marinum_M YAQGGPTHASNLKCYCRTHHLVKTFWG----WHDQQLPDGTLILTSPAGH
MAV_4456|M.avium_104 YAEGGPTHASNLKCYCRTHHLVKTFWG----WREQQLPDATLILTSPAGR
MUL_1813|M.ulcerans_Agy99 YGDGGHTHASNLKCLCRKHHLLKTFWG----WRDLQLPDGTVIWLLPDGH
MAB_4752|M.abscessus_ATCC_1997 WATGGPTTGANLKALCRKHHLLKTFGQ----WTELQEPDGTVIWKSPTGH
TH_0334|M.thermoresistible__bu YPAG-PTHPSNLKLLCVFHCRQAELRF----THDQHKHQSSCLPAYFQRP
Mflv_1180|M.gilvum_PYR-GCK HPVG-PTHASNLKCLCRFHHLLKTFWTGPGGWHDRQHPDGTITWTSPTGH
Mvan_0728|M.vanbaalenii_PYR-1 YPIG-PTHPSNLKCLCRFHHLLKTFWLGATGWHDRQHPDGTITWTAPTGH
MSMEG_5106|M.smegmatis_MC2_155 YPAG-PTHASNLKCLCRFHHLLKTFWTGPRGWTDRQHPDGTIVWTSPSGR
Mb2126|M.bovis_AF2122/97 WPLG-PTHPSNLKCACRKHHLLKTFWTG---WRDVQLPDGTVIWTAPNGH
Rv2100|M.tuberculosis_H37Rv WPLG-PTHPSNLKCACRKHHLLKTFWTG---WRDVQLPDGTVIWTAPNGH
* * :*** * * : : : :.:
MMAR_5187|M.marinum_M TYVTTPGS--ALLFPSLCHSTGGLPAAEADPP----------SDHCD---
MAV_4456|M.avium_104 TYVTTPGS--ALLFPSLCVPTG-EPARPVAAG----------SQPCG---
MUL_1813|M.ulcerans_Agy99 TYVTTPGS--ALLFPNLCVPTGELPPIVGTAGKPPRWPSGEDADPCPNPR
MAB_4752|M.abscessus_ATCC_1997 RYATTPVS--WFLFPALT--------------------------------
TH_0334|M.thermoresistible__bu HGPAT---------------------------------------------
Mflv_1180|M.gilvum_PYR-GCK TYITHPGS--RLLFPTLCKPTATL-WTGNPPQNP----------------
Mvan_0728|M.vanbaalenii_PYR-1 TYTTHPGS--RLLFPTLCQPTATL-WTGEPPQVP----------------
MSMEG_5106|M.smegmatis_MC2_155 QYTTVPGSHRRLSITELAAPTGALDLPATPIPTA----------------
Mb2126|M.bovis_AF2122/97 TYTTHPGS--RIFFPTWHTTTAEL--PQTSTAAV----------------
Rv2100|M.tuberculosis_H37Rv TYTTHPGS--RIFFPTWHTTTAEL--PQTSTAAV----------------
:
MMAR_5187|M.marinum_M --------QRSAMMP-----RRQRTR-------AQDRARRVATERRQNHH
MAV_4456|M.avium_104 --------ERTAMMP-----TRRRTR-------GQNRAARVAAERRANRA
MUL_1813|M.ulcerans_Agy99 TKPGESHRHRAPMQSQGTGSRRRRPRRPVRHPGAGNRSRRAAALLAATSL
MAB_4752|M.abscessus_ATCC_1997 ---------------------RHHTR-------QQARDRRRRTERNLNRE
TH_0334|M.thermoresistible__bu ---------------------RRPPS---------ARGLREAVPRQGVDP
Mflv_1180|M.gilvum_PYR-GCK -----TNNNRGSMMP-----RRTRTR-------AQNRATYITAERTRNAA
Mvan_0728|M.vanbaalenii_PYR-1 -----QSQQRGAMMP-----RRRNTR-------AHNHAAYITAERRRNAQ
MSMEG_5106|M.smegmatis_MC2_155 -----DPDLRGVKMP-----KRRRTR-------AQNTARTIAAERQLNDD
Mb2126|M.bovis_AF2122/97 -----NVDARGLMMP-----RRRRTR-------AAELAHRINAERALNDA
Rv2100|M.tuberculosis_H37Rv -----NVDARGLMMP-----RRRRTR-------AAELAHRINAERALNDA
* . .
MMAR_5187|M.marinum_M ARSTQPGGQPRSAAPPTPPQSEPQS-----------HPPPF---
MAV_4456|M.avium_104 ARLARATRPAPAASQSWPAGHGPDA-----------DPPPF---
MUL_1813|M.ulcerans_Agy99 GTCQIPRRIAPRPGPSRSSRGCTDSGTRMGESSMSARPRACVAG
MAB_4752|M.abscessus_ATCC_1997 QRLQREAALAALAAK---------------------DPPPF---
TH_0334|M.thermoresistible__bu RRVSGQ--------------------------------------
Mflv_1180|M.gilvum_PYR-GCK HRTDS--------PR-------------------GNDPPPF---
Mvan_0728|M.vanbaalenii_PYR-1 TRYATTGNTPPYLPRDHIPYQSSTG---WREPDYGNDPPPF---
MSMEG_5106|M.smegmatis_MC2_155 LVAEH------------------------------NKPPPF---
Mb2126|M.bovis_AF2122/97 YMAER------------------------------NKPPSF---
Rv2100|M.tuberculosis_H37Rv YMAER------------------------------NKPPSF---