For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
VTMPDWVTHAIWWQIYPLGFVGAHPADPPPGPEEHRLRRIVEWLDHAVELGASGLALGPVFASRTHGYDT TDHLRIDPRLGDDADFDHVVAEAHRRGLRVLLDGVFNHVGTDFARYRQALGGDADSAAWFRHRDGKFEVF EGHGELIALNHDNPEVVNYTAGVMRHWLGRGADGWRLDAAYAVPDRFWAQVLPIVRSDFGDAWFVGEVIH GDYAATVAASTFDSVTQYELWKAVWSALNDGNFHELDWALQRHNDFLASFVPMTFVGNHDVTRIASQLSD ARHLEHALVILFTTGGIPSVYAGDEWGHQGVKEERRGGDDAVRPEFGERPGPDAPGADVLRLHQYLIGLR RRHPWLHSATTTPLLLTNTQYVYRSAGAAGALVVALNLDDAPLRVALPELGLASGVVVAGSKAPPQEQIE VTEVPPHGWLVIEPR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_3576 | - | - | 100% (435) | alpha-amylase 3 |
| M. smegmatis MC2 155 | MSMEG_4696 | - | 2e-19 | 26.79% (433) | alpha-amylase family protein |
| M. smegmatis MC2 155 | MSMEG_6515 | treS | 4e-13 | 29.47% (207) | trehalose synthase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2498 | aglA | 2e-19 | 26.85% (406) | alpha-glucosidase AglA |
| M. gilvum PYR-GCK | Mflv_3333 | - | 1e-179 | 68.59% (433) | alpha amylase, catalytic region |
| M. tuberculosis H37Rv | Rv2471 | aglA | 3e-19 | 26.85% (406) | alpha-glucosidase AglA |
| M. leprae Br4923 | MLBr_02045 | - | 7e-24 | 63.53% (85) | hypothetical protein MLBr_02045 |
| M. abscessus ATCC 19977 | MAB_1565c | - | 4e-17 | 26.20% (416) | Alpha-amylase family protein |
| M. marinum M | MMAR_2753 | treS_1 | 1e-180 | 69.46% (429) | trehalose synthase TreS_1 |
| M. avium 104 | MAV_2842 | - | 0.0 | 69.48% (439) | alpha-amylase 3 |
| M. thermoresistible (build 8) | TH_2297 | - | 0.0 | 72.69% (443) | alpha-amylase 3 |
| M. ulcerans Agy99 | MUL_3001 | treS_1 | 0.0 | 68.74% (435) | trehalose synthase TreS_1 |
| M. vanbaalenii PYR-1 | Mvan_3059 | - | 0.0 | 71.33% (436) | alpha amylase, catalytic region |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_3576|M.smegmatis_MC2_155 -------------------------MTMPDWVTHAIWWQIYPLGFVGAHP
TH_2297|M.thermoresistible__bu -------------------------VTGPDWVRHTIWWQIYPLGFVGAHP
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 -------------------------MTGPDWVQHVIWWQVYPLGFVGAYP
MMAR_2753|M.marinum_M -------------------------------MQHAIWWQVYPLGFVGAFP
MUL_3001|M.ulcerans_Agy99 -------------------------MSKPTWVQHAIWWQVYPLGFVGAFP
Mflv_3333|M.gilvum_PYR-GCK -------------------------MSDPAWVEHVLWWQVYPLGFVGVHP
Mvan_3059|M.vanbaalenii_PYR-1 -------------------------MTEPDWVDHVVWWQVYPLGFVGAYP
Mb2498|M.bovis_AF2122/97 MDQHQRPDPMGPGSPRASARRPEPDPMGEPWWSRAVFYQVYPRSFADSNG
Rv2471|M.tuberculosis_H37Rv MDQHQRPDPMGPGSPRASARRPEPDPMGEPWWSRAVFYQVYPRSFADSNG
MAB_1565c|M.abscessus_ATCC_199 ---------------MTAAYLGQHD---DPWWSRAVFYQIYPRSFGDSDG
MSMEG_3576|M.smegmatis_MC2_155 -ADP---PPGPEEHRLRRIVEWLDHAVELGASGLALGPVFAS--RTHGYD
TH_2297|M.thermoresistible__bu WTDPTTGPPGPDEHRLRRVLDWLDHVVDLGASGLALGPVFAS--RSHGYD
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 LPQSSE-PPQPEHHRLRRLVDWFDHAIELGASGIALGPIFTS--RTHGYD
MMAR_2753|M.marinum_M ---ANQ-PPDPGEHRLRRLVGWFDHAVELGASGVALGPIFDS--RTHGYD
MUL_3001|M.ulcerans_Agy99 ---ANQ-PPNPGEHRLRRLVGWFDHAVELGASGVALGPIFDS--RTHGYD
Mflv_3333|M.gilvum_PYR-GCK ---ADT-APEPGEHRLRRIVDWLDYAVELGASGVALGPVFHS--RTHGYD
Mvan_3059|M.vanbaalenii_PYR-1 ---ADP-PPPPGEHRLRRIVDWLDYAIELGASGLALGPVFAS--RTHGYD
Mb2498|M.bovis_AF2122/97 ----------DGVGDLDGLASRLDHLQQLGVDAIWINPVTVSPMADHGYD
Rv2471|M.tuberculosis_H37Rv ----------DGVGDLDGLASRLDHLQQLGVDAIWINPVTVSPMADHGYD
MAB_1565c|M.abscessus_ATCC_199 ----------DGVGDIDGISAKLGYLDLLGVEGIWLNPVTRSPMADHGYD
MSMEG_3576|M.smegmatis_MC2_155 TTDHLRIDPRLGDDADFDHVVAEAHRRGLRVLLDGVFNHVGTDFARYRQA
TH_2297|M.thermoresistible__bu TTDHYRIDPRLGTDADFDELVEQAHRRGLRVLLDGVFHHVGTDFARYRDA
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 TTDHYRIDPRLGDDADFDHLIAQAHRRGLRVLLDGVFNHVGVDFPRYRAA
MMAR_2753|M.marinum_M TTDHFRIDPRLGDEGDFDHLVAEAHRRGLRVLLDGVFNHVGMDFHRYRHA
MUL_3001|M.ulcerans_Agy99 TTDHFRIDPRLGDEGDFDHLVAEAHRRGLRVLLDGVFNHVGVDFHSYRHA
Mflv_3333|M.gilvum_PYR-GCK TIDHYRVDPRLGDDSDFDHLIEESHSRGLRVLLDGVFNHVGTEFPRYREA
Mvan_3059|M.vanbaalenii_PYR-1 TTDHYRIDPRLGEDGDFDHLVAEARDRGLRILLDGVFNHVGTDFPRYRDA
Mb2498|M.bovis_AF2122/97 VADPRDIDPLFGGMPAFERLVAAAHRQGIKVTMDVVPNHTSSAHPWFQAA
Rv2471|M.tuberculosis_H37Rv VADPRDIDPLFGGMPAFERLVAAAHRQGIKVTMDVVPNHTSSAHPWFQAA
MAB_1565c|M.abscessus_ATCC_199 VSNPREIDPLFGGSIAMHRLIASAHRHNIKVIMDLVPNHTSSEHPWFLEA
MSMEG_3576|M.smegmatis_MC2_155 LGGD-----------------------------ADSAAWFRHRD-----G
TH_2297|M.thermoresistible__bu AAG-------------------------------GSSGWFRRRG-----D
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 IDSD----------------------------DHAAARWFRGSR-----G
MMAR_2753|M.marinum_M AD------------------------------DPSSAGWFRGRA-----G
MUL_3001|M.ulcerans_Agy99 AD------------------------------DPSSAGWFRGRA-----G
Mflv_3333|M.gilvum_PYR-GCK VD-------------------------------GGDRSWFATRG-----D
Mvan_3059|M.vanbaalenii_PYR-1 AA-------------------------------GGDSAWFRRRG-----G
Mb2498|M.bovis_AF2122/97 LADLPGSPARDRYFFRDGRGPDGSLPPNNWESVFGGPAWTRVREPDGNPG
Rv2471|M.tuberculosis_H37Rv LADLPGSPARDRYFFRDGRGPDGSLPPNNWESVFGGPAWTRVREPDGNPG
MAB_1565c|M.abscessus_ATCC_199 LQSPPGSDARERYIFRDGKGAHGELPPNNWPSVFGGPAWTRVTEADGTPG
MSMEG_3576|M.smegmatis_MC2_155 KFEVFEGHGELIALNHDNPEVVNYTAGVMRHWLGRGADGWRLDAAYAVPD
TH_2297|M.thermoresistible__bu RFETFEGHDELIALDHDNPEVAAYTVDVLRHWLNRGADGWRLDAAYAVPD
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 RFHTFEGHDGLITLNHDNPEVVDYTVDVMAHWLGRGADGWRLDAAYAVPE
MMAR_2753|M.marinum_M NFRTFEGHSELITLNHANPEVADYVVDVMAHWLQRGADGWRLDAAYAVPQ
MUL_3001|M.ulcerans_Agy99 NFRTFEGHSGLITLNHANPEVADYVVDVMAHWLQRGADGWRLDAAYAVPQ
Mflv_3333|M.gilvum_PYR-GCK DFATFEGHEGLITLDHDNADVVDYTVDVMSHWLARGADGWRLDAAYAVPD
Mvan_3059|M.vanbaalenii_PYR-1 DFATFEGHGDLIALDHRSAEVVDYTVDVMSHWLGRGADGWRLDAAYAVPD
Mb2498|M.bovis_AF2122/97 QWYLHLFDTEQPDLNSDNPEILDDFEKTLRFWLDRGVDGFRIDVAHGMAK
Rv2471|M.tuberculosis_H37Rv QWYLHLFDTEQPDLNWDNPEILDDFEKTLRFWLDRGVDGFRIDVAHGMAK
MAB_1565c|M.abscessus_ATCC_199 QWYLHLFAPEQPDVNWDNPEVFDDFADTLRFWLDRGIDGFRLDVAHGMAK
MSMEG_3576|M.smegmatis_MC2_155 R-----------------------------FWAQVLPIVRSDFGDAWFVG
TH_2297|M.thermoresistible__bu R-----------------------------FWARVLPDVRRSHPEAWFVG
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 T-----------------------------FWAATLPRVRDRYPQAWFVG
MMAR_2753|M.marinum_M Q-----------------------------FWAKVLPRVRDRYPRAWFVG
MUL_3001|M.ulcerans_Agy99 Q-----------------------------FWAKVLPRVRDRYPQAWFVG
Mflv_3333|M.gilvum_PYR-GCK R-----------------------------FWAQVLPRVRQAHPAAWFVG
Mvan_3059|M.vanbaalenii_PYR-1 S-----------------------------FWAGVLPRVRERHPQAWFVG
Mb2498|M.bovis_AF2122/97 PPGLPDSPDLGIEVLHHRDDDPRFNHPNVHAIHRDIRTVIDEYPGAVTVG
Rv2471|M.tuberculosis_H37Rv PPGLPDSPDLGIEVLHHRDDDPRFNHPNVHAIHRDIRTVIDEYPGAVTVG
MAB_1565c|M.abscessus_ATCC_199 PPGLPDIEDTETSMLHIADEDPRFNNEGVHEYHRKIRKVLDQYRDVVAVG
MSMEG_3576|M.smegmatis_MC2_155 EVIHGD---YAATVAASTFDSVTQYELWKAVWSALNDGNFHELDWALQRH
TH_2297|M.thermoresistible__bu EVIHGD---YAATVSAASFDSVTQYELWKAVWSSLNDGNFFELDWALQRH
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 ELIHGD---YAAVVRAAGFDSATQYELWKAIWSSLNDGNFYELDWALQRH
MMAR_2753|M.marinum_M EIIHGD---YAAAVQGAGFDSVTQYELWKAIWSGLNDGNFFELDWALQRH
MUL_3001|M.ulcerans_Agy99 EIIHGD---YAAAVQGAGFDSVTQYELWKAIWSGLNDGNFFELDWALQRH
Mflv_3333|M.gilvum_PYR-GCK EVIHGD---YVARVRDSGFDSVTQYELWKAVWSSINDANFHELDWALTRH
Mvan_3059|M.vanbaalenii_PYR-1 EVIHGD---YPARVRDSGFDSVTQYELWKAIWSSLNDGNFHELDWALTRH
Mb2498|M.bovis_AF2122/97 EVWVHDNARWAEYLRPDELHLGFNFRLARTEFDAAEIRDAVANSLAAAAL
Rv2471|M.tuberculosis_H37Rv EVWVHDNARWAEYLRPDELHLGFNFRLARTEFDAAEIRDAVANSLAAAAL
MAB_1565c|M.abscessus_ATCC_199 EIWVNDNTRFAEYVRPDELHLGFNFKLVEADFDADQIRAAIQNSLAAVDS
MSMEG_3576|M.smegmatis_MC2_155 NDFLASFVPMTFVGNHDVTRIASQLSDARHLEHALVILFTTGGIPSVYAG
TH_2297|M.thermoresistible__bu NDFLAVFTPLTFIGNHDVTRIASRLADRRHVEHAVVLLLTVGGVPSIYAG
MLBr_02045|M.leprae_Br4923 -----------------MSRIASQLGQPEHLTHALIILLTVAGVPSVYAG
MAV_2842|M.avium_104 NAFLGRFSPLTFIGNHDVTRIASQLNNPAHLAHALVLLLTVGGVPSVYAG
MMAR_2753|M.marinum_M NEFVASFAPLTFIGNHDVTRIASRLERPEHLAHALVILMTVGGIPSIYAG
MUL_3001|M.ulcerans_Agy99 NEFVASFAPLTFIGNHDVTRIASRLERPEHLAHALVILMTVGGIPSIYAG
Mflv_3333|M.gilvum_PYR-GCK NEFLDTFVPLTFIGNHDVTRIASRLTDPAHVEHALVVLLTTGGTPSVYAG
Mvan_3059|M.vanbaalenii_PYR-1 DEFLDTFVPMTFVGNHDVTRIASQLTDPTHVEHALVLLMTVGGTPSVYAG
Mb2498|M.bovis_AF2122/97 QNATPTWTLANHDVGREVSRYGGGEIGLRRAKAMAVVMLALPGVVFLYNG
Rv2471|M.tuberculosis_H37Rv QNATPTWTLANHDVGREVSRYGGGEIGLRRAKAMAVVMLALPGVVFLYNG
MAB_1565c|M.abscessus_ATCC_199 VGATPTWTLSNHDVEREVTRYGDGQIGQWRARAMALVMLALPGTVFIYNG
::* .. : ::::: * :* *
MSMEG_3576|M.smegmatis_MC2_155 DEWGHQGVKE----------ERRGGDDAVRPEFGERP---------GPDA
TH_2297|M.thermoresistible__bu DEAGYLGVKE----------ERFSGDDAIRPEFGSPPIVS----GAGENT
MLBr_02045|M.leprae_Br4923 DEFGFRGLKE----------ERYGVDDAVLPEFFSPP--------MQLDV
MAV_2842|M.avium_104 DELGFRGVKE----------ERFGGDDAVRPEFGSPP--------IPLDD
MMAR_2753|M.marinum_M DEFGWRAVKE----------ERYGGDDAIRPEFGSPP--------LQLTH
MUL_3001|M.ulcerans_Agy99 DEFGWRAVKE----------ERCGGDDAIRPEFGSPP--------FELTH
Mflv_3333|M.gilvum_PYR-GCK DEVAYRGVKE----------ERFGGDDAVRPEFRSPF--------DGVGE
Mvan_3059|M.vanbaalenii_PYR-1 DEVAYRGVKE----------ERFGGDDAVRPEFGSPF--------EGVGE
Mb2498|M.bovis_AF2122/97 QELGLPDVDLPDEVLQDPTWERSGRTERGRDGCRVPIPWSGNIPPFGFST
Rv2471|M.tuberculosis_H37Rv QELGLPDVDLPDEVLQDPTWERSGRTERGRDGCRVPIPWSGNIPPFGFST
MAB_1565c|M.abscessus_ATCC_199 SELGLPNVELPDEALQDPVWERSGHTERGRDGCRVPIPWEGTDPPYGFSS
.* . :. ** . :
MSMEG_3576|M.smegmatis_MC2_155 PGADVLRLHQYLIGLRRRHPWLHSATTTPLLLTNTQYVYRSAGAAGALVV
TH_2297|M.thermoresistible__bu VAADLVRLHQHLIGLRRRNPWLHTATTAPLHLTNTQYVYESRSGEHALVV
MLBr_02045|M.leprae_Br4923 FGVHAWALHQYLIGLRRRHP------------------------------
MAV_2842|M.avium_104 FGTEVWRLHQYLIGLRRRHPWLHAATTTALLLQNRHYVYETRSGDEALLI
MMAR_2753|M.marinum_M DQASVWALHQYLVGLRRRYPWLHTASTSALRLRNRQYVYQTRSADNALVV
MUL_3001|M.ulcerans_Agy99 DQASVWALHQYLVGLRRRYPWLHTASTSALRLRNRQYVYQTRSADNALVV
Mflv_3333|M.gilvum_PYR-GCK QGHQMFRSHQHLIGLRRRHPWLHEARTTALSVTNTGYVYITRSGDDALVV
Mvan_3059|M.vanbaalenii_PYR-1 HGHQVFRSHQHLIGLRRRNPWLHTARTTPLRVTNRGYVYTTRHGDDALVV
Mb2498|M.bovis_AF2122/97 CPDTWLPMPPEWAALTAEKQRADAGSTLSFFRLALRLRRERNEFDG-DVD
Rv2471|M.tuberculosis_H37Rv CPDTWLPMPPEWAALTAEKQRADAGSTLSFFRLALRLRRERNEFDG-DVD
MAB_1565c|M.abscessus_ATCC_199 NAQTWLPMPHGWAEYTVERQLEHTDSMLSLYRRAIELRKSRKEFTGTSLE
.
MSMEG_3576|M.smegmatis_MC2_155 ALNLDDAPLRVALPELGLASGVVVAGSKAPPQEQIEVTEVPPHGWLVIEP
TH_2297|M.thermoresistible__bu ALNIDTAPLPLRLSEIGLGAGRVVAGSGAPPAADLTNVDVAPHGWLIIEP
MLBr_02045|M.leprae_Br4923 --------------------------------------------------
MAV_2842|M.avium_104 VLNIGDEPLRVSLDELGGRRGRVIGGSAAPPPEVLDTVVVEPQGWRILSP
MMAR_2753|M.marinum_M ILNADDEPLRMSLPDLGIPRAEVLAGSAAPPQEVTDEVLVEGHGWRILQP
MUL_3001|M.ulcerans_Agy99 ILNADDEPLRMSLPDLGIPHAEVLAGSAAPPQEVTDEVLVEGHGWRILQP
Mflv_3333|M.gilvum_PYR-GCK ALNVADEPLPVALSGLGLGHAEIVAGSGAPPAEVVPDIVVPPHGWVILNP
Mvan_3059|M.vanbaalenii_PYR-1 ALNIDDDAMPVSLAEFGLARAEVVAGSGAPPAQVVADTVVPAHGWLILAP
Mb2498|M.bovis_AF2122/97 WLAAPDDALIFRRHGGGLVCALNAAERPLALPAGEPILASAPLTDATLPP
Rv2471|M.tuberculosis_H37Rv WLAAPDDALIFRRHGGGLVCALNAAERPLALPAGEPILASAPLTDATLPP
MAB_1565c|M.abscessus_ATCC_199 WYGSPPGALAFRVKGGGLTCALNVSSDLVDLPEGEVILTSGPLVNGKLPR
MSMEG_3576|M.smegmatis_MC2_155 R---------------
TH_2297|M.thermoresistible__bu AR--------------
MLBr_02045|M.leprae_Br4923 ----------------
MAV_2842|M.avium_104 I---------------
MMAR_2753|M.marinum_M A---------------
MUL_3001|M.ulcerans_Agy99 ASRFSCASVSGSSRRA
Mflv_3333|M.gilvum_PYR-GCK R---------------
Mvan_3059|M.vanbaalenii_PYR-1 R---------------
Mb2498|M.bovis_AF2122/97 NAAAWLV---------
Rv2471|M.tuberculosis_H37Rv NAAAWLV---------
MAB_1565c|M.abscessus_ATCC_199 NTAAWIV---------