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M. smegmatis MC2 155 MSMEG_3403 (amiF)

annotation: formamidase
coordinates: 3476015 - 3477016
length: 333

MTGLDGLNPTPGALVLGLVQARVPVIKEPGDLKSTAERLAGMVVGAKKGMPSIDLVVMPEYSINGLDPDT
WLDDSLLCDRNGPEMTLLADACRDAGVWGCFSIMERNRGGAPWNSGIIIDDHGEERLYYRKMHPWVPAEP
WAPGDLGVPVCDGPSGSRLALIICHDGMLPEMAREAAYKGANVILRTAGYTYPIQHSWRITNQTNAFHNL
AYTASVALAGPDQNNIWSQGEAMVCDVDGTILVSGDGTPDRIVTAEVVPSRADEARRTWGVENNIYQLGH
RGYTAVTGGAQDCPYTFMQDLVAGTYRLPWEDEVRHVDGTGAGWGAPETVRAK
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_3403amiF-100% (333)formamidase
M. smegmatis MC2 155MSMEG_6733-1e-1129.32% (266) hydrolase, carbon-nitrogen family protein
M. smegmatis MC2 155MSMEG_0443-9e-0725.30% (249) hydrolase, carbon-nitrogen family protein

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97-----
M. gilvum PYR-GCKMflv_0095-3e-0525.58% (215) Nitrilase/cyanide hydratase and apolipoprotein
M. tuberculosis H37Rv-----
M. leprae Br4923-----
M. abscessus ATCC 19977MAB_0819-3e-1327.67% (253) carbon-nitrogen hydrolase family protein
M. marinum MMMAR_4512-3e-0525.91% (220) amidohydrolase
M. avium 104MAV_4670-5e-0624.86% (173) carbon-nitrogen hydrolase
M. thermoresistible (build 8)-----
M. ulcerans Agy99-----
M. vanbaalenii PYR-1Mvan_3160-1e-0926.22% (267) Nitrilase/cyanide hydratase and apolipoprotein

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_0095|M.gilvum_PYR-GCK          ---------MRSGMRIALAQIQS-STDPAANLTLVEEYTRRAVDAGAS--
MAV_4670|M.avium_104                -------------MRIALAQILS-GTDPAANLALVGEYTRRAAGAGAR--
MAB_0819|M.abscessus_ATCC_1997      ---------MTGRLRLAVVQCSSQAADVQANLARLAETAHAAAAGDAQ--
MMAR_4512|M.marinum_M               ---------MHQVETELVVAVAQPRTEMGAVAANVDAHAALIRRANAR--
MSMEG_3403|M.smegmatis_MC2_155      MTGLDGLNPTPGALVLGLVQARVPVIKEPGDLKSTAERLAGMVVGAKKGM
Mvan_3160|M.vanbaalenii_PYR-1       ----------MAPSTYTLAMAQPRRIAGPDGSANVAHAVRLVEQAAGAD-
                                                     :.                         .     

Mflv_0095|M.gilvum_PYR-GCK          ----LVLFPEATMVRFGV---PLGGVAEPFDGPWANAVRDIAARAGIVVV
MAV_4670|M.avium_104                ----LVVFPEATMCRFGV---PLAPIAEPVDGPWADGVRRIATEANVTVI
MAB_0819|M.abscessus_ATCC_1997      ----LVVFPEMYLTGYNIGEWDIRDLAQSPDGPAMQFIRQTACDADMHIC
MMAR_4512|M.marinum_M               ----LVVFPELSLTGYQL-----KVAPVDVAGPVVDRLVLACAATGCSVL
MSMEG_3403|M.smegmatis_MC2_155      PSIDLVVMPEYSINGLDPDTWLDDSLLCDRNGPEMTLLADACRDAGVWGC
Mvan_3160|M.vanbaalenii_PYR-1       ----LVCFPEFSPG---------PVRAHDTFYDAGPALAAAARACDVNVV
                                        ** :**                           :   .   .    

Mflv_0095|M.gilvum_PYR-GCK          AGMFVPSDDGRSFNTLLATGPG--VEARYDKIHLYDAFGFLESKTVAPGR
MAV_4670|M.avium_104                AGMFTPSGDGRVKNTLLVANSDDQAVTHYDKIHLYDAFGFTESRTVAPGR
MAB_0819|M.abscessus_ATCC_1997      VGYPERASDGQIYNSALICDPRGRVVLNYRKSHLFGDT--ERNVFTRPGP
MMAR_4512|M.marinum_M               VGAPVEDRGRRYIAMVLVDSSG--AEVVYRKTHL----GAAEKARFRPGP
MSMEG_3403|M.smegmatis_MC2_155      FSIMERNRGGAPWNSGIIIDDHGEERLYYRKMHP-----WVPAEPWAPG-
Mvan_3160|M.vanbaalenii_PYR-1       WSRTERCDDGRNRLVVYVTGRDGDPVLRYARTHPATIPPGEDGELVSPAD
                                     .      .          .        * : *              *. 

Mflv_0095|M.gilvum_PYR-GCK          EPVVISVDTESGPVTVGLTLCYDIRFPELYVELADRGAEIITAHASWGTG
MAV_4670|M.avium_104                EPVVVGVDG----VRVGLSVCYDIRFPELYTELARRGAQLIAVCASWGAG
MAB_0819|M.abscessus_ATCC_1997      QLPLAVVHG----VSVGLLICYDVEFPENVRALAMARADVVVVPTAN--M
MMAR_4512|M.marinum_M               GPRTIDLDG----WRIGLGICRDTGVPEHVRGTARLGVDLYACGVVHHDW
MSMEG_3403|M.smegmatis_MC2_155      DLGVPVCDGPSG-SRLALIICHDGMLPEMAREAAYKGANVILRTAGY--T
Mvan_3160|M.vanbaalenii_PYR-1       TFGNFEVDG----IPMGIVVCSEMWTPEIARIVALRGAEIILSPAGGG-F
                                           .       :.: :* :   **     *   .::    .     

Mflv_0095|M.gilvum_PYR-GCK          PGKLEQWTLLARARAIDTSSVVAAVGQAYPG--DEIAALG-PTGVGGSLV
MAV_4670|M.avium_104                PGKLDQWTLLARARALDSMSYIAAAGQADPG--GSLSASGAPTGVGGSLV
MAB_0819|M.abscessus_ATCC_1997      EPFHAVCETVVPARAYENQLYVAYANYCGNH--DGLAYCG------GSSI
MMAR_4512|M.marinum_M               ELGEQRHRARTIAATCDAPVAMASFAGPTGG--GYLHTAG------HSAI
MSMEG_3403|M.smegmatis_MC2_155      YPIQHSWRITNQTNAFHNLAYTASVALAGPDQ-NNIWSQG------EAMV
Mvan_3160|M.vanbaalenii_PYR-1       TSLTRNWQIIVSARAIENLCYIGLTNNLWGDEQGAAMITG-----PEHPL
                                                : : .     .          .     *         :

Mflv_0095|M.gilvum_PYR-GCK          ASALGEVLASAGDDP-ELLVCDVDLEAARKARQTVAVMQNRSGFAHRGKA
MAV_4670|M.avium_104                ASPLGEVVASAGAQP-QLVLADIDVDRVAQARKTIAALSNRSDFAQVGRA
MAB_0819|M.abscessus_ATCC_1997      VGPDGTALARAGEGV-GLLVADVDRAVIDRTRSRHCFLEDR-----VARA
MMAR_4512|M.marinum_M               WSATSTLVAEAGAGPDQIAHTTIGRPPPGRAGDDHTPKTA----------
MSMEG_3403|M.smegmatis_MC2_155      CDVDGTILVSGDGTPDRIVTAEVVPSRADEARRTWGVENNIYQLGHRGYT
Mvan_3160|M.vanbaalenii_PYR-1       AHAGRSELVTATVDLDRLRWLRAHDDSMAEPKAFDSIPGLLRARRPELYG
                                           :. .      :           ..                   

Mflv_0095|M.gilvum_PYR-GCK          ESRG---------------------------------------------
MAV_4670|M.avium_104                ESVG---------------------------------------------
MAB_0819|M.abscessus_ATCC_1997      ISP----------------------------------------------
MMAR_4512|M.marinum_M               -------------------------------------------------
MSMEG_3403|M.smegmatis_MC2_155      AVTGGAQDCPYTFMQDLVAGTYRLPWEDEVRHVDGTGAGWGAPETVRAK
Mvan_3160|M.vanbaalenii_PYR-1       ELVAPQADAYDYANGQA--------------------------------