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PDQRSESGRFDVEVDVLVAGSGGGVAGAYTAAREGLSVLLVEATDKFGGTTAFSGGGGMWFPCNPVLERA GTDDTLDEALKYFHAVVGDRTPQELQDAYVTGGAGFIAYLEQDHGFEFAVLPWPDYYGSVPGARNDGYRH IVPKPLPDSALGSYQGLVRGPLDTERLGAPAPDTLIGGRALVGRFLAALDKLPNADCWCEAPLTELITES GRVVGAIVERGGERLRVCARRGVLLASGGFEQNADMRGRYGVPGSATDTMGGPGSTGAAHRAAMAVGADV DLMDQAWWSPGLTHPDGRSAFALWFTGGIFVDQDGKRFVNESAPYDRLGRAVIERLESGRLTLPYWMVYD SRAGDVPPVGATNVSMVDPAEYRAAGLWRSAETISGLAEEIGVPADALEATIQRFNEMATAGHDDDFGRG DEAYDRVFTGGASPLVPIDTPPYHAAAFGLSDLGTKGGLRTDTRARVRGRDGEPIPGLYAAGNTMAAVSG TTYPGGGNPIGASMLFSHFAALDMAAEGTTA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2867 | - | - | 100% (522) | 3-ketosteroid-delta-1-dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_4864 | - | 6e-80 | 36.65% (573) | 3-ketosteroid-delta-1-dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_5941 | - | 1e-79 | 36.11% (565) | 3-ketosteroid-delta-1-dehydrogenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3567 | - | 2e-77 | 36.92% (558) | 3-ketosteroid-delta-1-dehydrogenase |
| M. gilvum PYR-GCK | Mflv_1547 | - | 0.0 | 65.70% (516) | 3-ketosteroid-delta-1-dehydrogenase |
| M. tuberculosis H37Rv | Rv3537 | - | 2e-77 | 36.92% (558) | 3-ketosteroid-delta-1-dehydrogenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0081 | - | 1e-76 | 34.51% (565) | 3-ketosteroid-delta-1-dehydrogenase |
| M. marinum M | MMAR_4775 | - | 2e-80 | 36.75% (566) | dehydrogenase |
| M. avium 104 | MAV_4249 | - | 0.0 | 68.70% (508) | 3-ketosteroid-delta-1-dehydrogenase |
| M. thermoresistible (build 8) | TH_3671 | - | 1e-156 | 80.94% (320) | PUTATIVE fumarate reductase/succinate dehydrogenase |
| M. ulcerans Agy99 | MUL_0346 | - | 2e-81 | 37.17% (565) | 3-ketosteroid-delta-1-dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_2865 | - | 0.0 | 80.59% (505) | 3-ketosteroid-delta-1-dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_2867|M.smegmatis_MC2_155 MPDQRSE-----SGRFDVEVDVLVAGSG-GGVAGAYTAAREGLSVLLVEA
Mvan_2865|M.vanbaalenii_PYR-1 MPD------------FDADFDVVVAGSG-GGIAGAYTAAREGLSVLLVEA
Mflv_1547|M.gilvum_PYR-GCK MAPLESPGEEP-TTTWDEVTDVLVAGSGAGGVTGAYTAAREGLDVILVEA
MAV_4249|M.avium_104 MG----------TVQWTAECDVLVAGSGAGGVTGAYTAAREGLEVLLVEA
TH_3671|M.thermoresistible__bu MSD-------------DIVVDVLVAGSG-GGLAGVYTAARAGLSVLLVEA
Mb3567|M.bovis_AF2122/97 MTVQ--------------EFDVVVVGSGAAGMVAALVAAHRGLSTVVVEK
Rv3537|M.tuberculosis_H37Rv MTVQ--------------EFDVVVVGSGAAGMVAALVAAHRGLSTVVVEK
MMAR_4775|M.marinum_M MTVGPWGDVDR-------MVDVLVVGSGGGGMTAALTAASSGLDTLVIEK
MUL_0346|M.ulcerans_Agy99 MTVGPWGDVDR-------MVDVLVVGSGGGGMTAALTAASSGLDTLVIEK
MAB_0081|M.abscessus_ATCC_1997 MASAPPGLIPAGTVAGDIEIDLLIVGAG-TGMAAALAASELGLSVLIVEK
* *:::.*:* *:... .*: **..:::*
MSMEG_2867|M.smegmatis_MC2_155 TDKFGGTTAFSGGGGMWFPCNPVLERAGTDDTLDEALKYFHAVVGDRTPQ
Mvan_2865|M.vanbaalenii_PYR-1 TDKFGGTTAYSGGGGTWYPCNPVLRRAGTDDTIAAALDYFHAVVGDRTPR
Mflv_1547|M.gilvum_PYR-GCK TEKFGGTTAYSGGGGMWFPCNPVLMRAGVDDTIEDALEYYTSVVGDRTPR
MAV_4249|M.avium_104 TSKFGGTTAYSGGGGVWFPCNPVLIRAGTDDTIEDALTYYHAVVGDRTPR
TH_3671|M.thermoresistible__bu TDKFGGTTAYSGGGGMWYPCNPVVRRAGTDDTIADALEYFHAVVGDRTPR
Mb3567|M.bovis_AF2122/97 APHYGGSTARSGGG-VWIPNNEVLKRRGVRDTPEAARTYLHGIVGEIVEP
Rv3537|M.tuberculosis_H37Rv APHYGGSTARSGGG-VWIPNNEVLKRRGVRDTPEAARTYLHGIVGEIVEP
MMAR_4775|M.marinum_M SSCFGGSTALSGGG-IWVPGAPAQRRAGYSPNPDDVVGYLRRITDGLVSE
MUL_0346|M.ulcerans_Agy99 SSCFGGSTALSGGG-IWVPGDPAQRRAGYSPNPDDVVGYLRRITNGLVSE
MAB_0081|M.abscessus_ATCC_1997 SHQVGGSTARSGGA-LWLPASPVLRAAGTGDSVERARTYLRSVVAGSAPD
: **:** ***. * * . * . . * :. .
MSMEG_2867|M.smegmatis_MC2_155 ELQDAYVTGGAGFIAYLEQDHG-FEFAVLP-WPDYYGSVPGARNDGYRHI
Mvan_2865|M.vanbaalenii_PYR-1 DLQDTYVRGGAPFIEYLEQDPG-FEFMVLP-WPDYYGSAPGARDDGYRHI
Mflv_1547|M.gilvum_PYR-GCK ALQETYVRSGAPLVEYLEADEL-INFSLLP-WPDYFGAAPKARADGMRHI
MAV_4249|M.avium_104 ELQETYVRGGAPLIEYLEADPH-LAFVPLP-WPDYYGKAPKARLDGQRHI
TH_3671|M.thermoresistible__bu DLQDAYVTGGAGFIEFLEQDPH-FEFTVLP-WPDYYGSAPGARNDGYRHI
Mb3567|M.bovis_AF2122/97 ERIDAYLDRGPEMLSFVLKHTP-LKMCWVPGYSDYYPEAPGGRPGG-RSI
Rv3537|M.tuberculosis_H37Rv ERIDAYLDRGPEMLSFVLKHTP-LKMCWVPGYSDYYPEAPGGRPGG-RSI
MMAR_4775|M.marinum_M ARIRQYVYSAPQMLEFLEGLSAWLEFVWKPGYADYYPELPGGSVLG-STI
MUL_0346|M.ulcerans_Agy99 ARIRQYVYSAPQMLEFLEGLSAWLEFVWKPGYADYYPELPGGSVLG-STI
MAB_0081|M.abscessus_ATCC_1997 ARSDAFLEHEAATVDMLTRLTP-MRFTWCRDYADYHPEEPGGSPTG-RTC
:: . : : : : :.**. * . *
MSMEG_2867|M.smegmatis_MC2_155 VPKPLPDSALGSYQGLVRGPLDTERLG-----------------------
Mvan_2865|M.vanbaalenii_PYR-1 IPLPLPDSDLGRYAGQVRGPLDTERLG-----------------------
Mflv_1547|M.gilvum_PYR-GCK AAKPWKVAAAPHLRELVRGPLDSDRLG-----------------------
MAV_4249|M.avium_104 AAKPLPVAAAPEFRTVIRGPLDTDRLG-----------------------
TH_3671|M.thermoresistible__bu VPVPLPDDALGRHAGLVRGPLDTERLG-----------------------
Mb3567|M.bovis_AF2122/97 EPKPFNARKLGADMAGLEPAYGKVPLN-VVVMQQDYVRLNQLKRHPR-GV
Rv3537|M.tuberculosis_H37Rv EPKPFNARKLGADMAGLEPAYGKVPLN-VVVMQQDYVRLNQLKRHPR-GV
MMAR_4775|M.marinum_M NVPPIDLRKLGDDQPRLLTPLALAPRG-IWLGPKELRSF-YRIRQSWAGK
MUL_0346|M.ulcerans_Agy99 NVPPIDLRKLGDDQPRLLTPLALAPRG-IWLGPKELRSF-YRIRQSWAGK
MAB_0081|M.abscessus_ATCC_1997 ECRPFDASVLGEYRTRLQPGVMKPPAVPMPVTSADYRWMNLMARLPRKAL
* :
MSMEG_2867|M.smegmatis_MC2_155 -------------APAP-DTLIGGRALVGRFLAALDKLPNADCWCEAPLT
Mvan_2865|M.vanbaalenii_PYR-1 -------------TPAP-DVLAGGRALVGRFLAALDKLPNVTCWRNAPLE
Mflv_1547|M.gilvum_PYR-GCK -------------APQPDDYFVGGRALIARLLAAAQRHPNSSARLNTALV
MAV_4249|M.avium_104 -------------AEPPSDYYLGGRALIARFLRAIGEFPTATLRRDTALV
TH_3671|M.thermoresistible__bu -------------TPAP-DVLVGGRALVGRFLAALGGFPDVTCWRNTPLT
Mb3567|M.bovis_AF2122/97 LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTD
Rv3537|M.tuberculosis_H37Rv LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTD
MMAR_4775|M.marinum_M AVLLKLVTRMIRARVFGEQIAAIGQSLAARLRLAMKELGVALWLDAPLVE
MUL_0346|M.ulcerans_Agy99 AVLLKLVTRMIRARVFGEQIAAIGQSLAARLRLAMKELGVALWLDAPLVE
MAB_0081|M.abscessus_ATCC_1997 PLIVKRIAQGVGGAVLGRRYLAGGQALAAGLFAGVLRAGIPIWTGTSLVR
*::* . : . .
MSMEG_2867|M.smegmatis_MC2_155 ELITESGRVVGAIVERGGERL-----RVCARRGVLLASGGFEQNADMRGR
Mvan_2865|M.vanbaalenii_PYR-1 ELITDDGRVVGAMIVHDGTPV-----RIRTRRGVLLASGGFEQNADMRTA
Mflv_1547|M.gilvum_PYR-GCK ELVVEDGAVVGAVVDADGERR-----SIRTRRGVLLAAGGFEHNDDMRAR
MAV_4249|M.avium_104 ELVRTDGRVVGAVVETGGERR-----AVRARRGVLLAAGGFEANPDLRRA
TH_3671|M.thermoresistible__bu ELICDGDRVVGAVVVRDGTPV-----RVRARRGVLLAAGGFERNADMRAR
Mb3567|M.bovis_AF2122/97 LFVENG-VVSGVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIK
Rv3537|M.tuberculosis_H37Rv LFVENG-VVSGVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIK
MMAR_4775|M.marinum_M LLTDAGGAVTGAVVQTEGGRE-----RIGVRGGVILASGGFDHDLAWRKE
MUL_0346|M.ulcerans_Agy99 LLTDAGGAVTGAVVQTEGGRE-----RIGVRGGVILASGGFDHDLAWRKE
MAB_0081|M.abscessus_ATCC_1997 -LTTAGGRVTGAVVALAGREF-----TVTVSRGVVLAAGGFDHDMEMRHK
: . * *. : : . **:**.***: : *
MSMEG_2867|M.smegmatis_MC2_155 YG-VPGSATDTMGGPGSTGAAHRAAMAVGADVDLMDQAWWSPGLT-HPDG
Mvan_2865|M.vanbaalenii_PYR-1 YG-VPGSANDTMGGPGSTGGAHRAAIAVGADLDLMDQAWWSPGMT-HPDG
Mflv_1547|M.gilvum_PYR-GCK YG-VPGSSRDTMGPWGNRGLAHQAGIAVGADTDLMDQAWWSPGLT-HPDG
MAV_4249|M.avium_104 YG-VPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLT-HPDG
TH_3671|M.thermoresistible__bu YG-VPGDATDTMGAPGNTGAAHLAAIAAGADTDLMDQAWWSPGLT-HPDG
Mb3567|M.bovis_AF2122/97 YQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWGPTVP--LVG
Rv3537|M.tuberculosis_H37Rv YQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWGPTVP--LVG
MMAR_4775|M.marinum_M HQ-PLVEQDWSFGNPAALGDGIRAGQRVGAAADLLDEAWWFPAIQ-WPDG
MUL_0346|M.ulcerans_Agy99 HQ-PLVEQDWSFGNPAALGDGIRAGQRVGAAADLLDEAWWFPAIQ-WPDG
MAB_0081|M.abscessus_ATCC_1997 FQSAALGENMSLGADTNTGDALRIGQDCGAAIDSMNQAWWFPAVAPLPGG
. :.* * . . ** * :::*** * : *
MSMEG_2867|M.smegmatis_MC2_155 RSAFALWFT---GGIFVDQDGKRFVNESAPYDRLGRAVIERLESGRL---
Mvan_2865|M.vanbaalenii_PYR-1 RSAFALWFT---GGIFVNQDGHRFVNESAPYDRLGREVIRQIDSGAT---
Mflv_1547|M.gilvum_PYR-GCK TSAFALWFT---GGIFVDDHGRRFVNESAAYDRLGRAVLAAVDDGRV---
MAV_4249|M.avium_104 RSAFALWFT---GGIFVNQSADRFVNESMAYDRAGRQILAHLRDGSL---
TH_3671|M.thermoresistible__bu RSAFALWFT---GGIFVDQNGRRFVNESAPYDRLGREGXXXAGGHRV---
Mb3567|M.bovis_AF2122/97 KPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPG
Rv3537|M.tuberculosis_H37Rv KPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPG
MMAR_4775|M.marinum_M RMQFMLNERMMPAQFIVNGAGKRFINEAAPYMDFGHAMIAGQKSGVT---
MUL_0346|M.ulcerans_Agy99 RMQFMLNERMMPAQFIVNGAGKRFINEAAPYMDFGHAMIAGQKSGVT---
MAB_0081|M.abscessus_ATCC_1997 APKVMLAERSLPGSFIVDHTGNRFINEATDYMSFGQRVLERERSGDP---
. * . ::*: . **:**: * : .
MSMEG_2867|M.smegmatis_MC2_155 -TLPYWMVYD---SRAGDVPPVGATNVSMVD--------PAEYRAAGLWR
Mvan_2865|M.vanbaalenii_PYR-1 -TPPFWMVYD---DRAGDIPPVSATNVSMVE--------AAQYREAGLWH
Mflv_1547|M.gilvum_PYR-GCK -TLPFWMIYDSAGDRDGVVPPVKATNVSMVE--------PEKYIAAGLWR
MAV_4249|M.avium_104 -RLPYWMIYD---DKEGAVPPVKATNVSLVE--------TEKYVAAGLWH
TH_3671|M.thermoresistible__bu -GEP----------GQRGAAAVHAEHLGRDL--------PVDHPAEELH-
Mb3567|M.bovis_AF2122/97 ENIPAWLVFDQRYRDRYIFAGLQPGQRIP-----------SRWLDSGVIV
Rv3537|M.tuberculosis_H37Rv ENIPAWLVFDQRYRDRYIFAGLQPGQRIP-----------SRWLDSGVIV
MMAR_4775|M.marinum_M -HIPCWLVTDHRSFNRYVVAGHLPIPKIPGAPVPTGRKIPTAWLESGVVK
MUL_0346|M.ulcerans_Agy99 -HIPCWLVTDHRSFNRYVVAGHLPIPKIPGAPVPTGRKIPTAWLESGVVK
MAB_0081|M.abscessus_ATCC_1997 -AESMWIIFDQQYRNSYVFAGQL-YPRMP---------VPVSWFEARIAY
. . :
MSMEG_2867|M.smegmatis_MC2_155 SAETISGLAEEIGVPADALEATIQRFNEMATAGHDDDFGRGDEAYDRVFT
Mvan_2865|M.vanbaalenii_PYR-1 TADTIEGLAEAIGVPPAELKYTIERFNTLAAAGVDEDFGRGGEAYDRVFT
Mflv_1547|M.gilvum_PYR-GCK SADTLEELAVQIGVPPDNLVDSVARFNDFVRRGVDEDFGRGDEPYDRAFS
MAV_4249|M.avium_104 TADSLPELAAKIGVPAQRLTATVLRFNGFAAAGVDEDFGRGDEAFDRAFS
TH_3671|M.thermoresistible__bu -ADRAHQGSGERIVDHGILGAARQRAG-------GRHHGRGG-----ADA
Mb3567|M.bovis_AF2122/97 QADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYG
Rv3537|M.tuberculosis_H37Rv QADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYG
MMAR_4775|M.marinum_M AATSWAELATKVGVPGGQLQATAERFNELAVKGHDEDFNRGDSVYDNYYG
MUL_0346|M.ulcerans_Agy99 AATSWAELATKIGVPGGQLQATAERFNELAVYGHDDDFNRGDSVYDNYYG
MAB_0081|M.abscessus_ATCC_1997 RADSLADLAAMIGIPRDAFLATVCRFNESAAAGDDGDFHRGRSSYDRYYG
* : : : : * . . .. **
MSMEG_2867|M.smegmatis_MC2_155 GGAS----PLVPIDTPPYHAAAFGLSDLGTKGGLRTDTRARVRGRDGEPI
Mvan_2865|M.vanbaalenii_PYR-1 GGSS----PLVPIDTPPYHAAAFGLSDLGTKGGLRTDTRARVLTADGTPI
Mflv_1547|M.gilvum_PYR-GCK GGVP----PLYAIEKGPFHAAAFGVSDLGTKGGLRTDTAARVLDRADNVI
MAV_4249|M.avium_104 AGAS----PLVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPI
TH_3671|M.thermoresistible__bu GGQV----PGVVVGTRHRRPLTDQCTNPELVS--CHNSSSRLYARFRGPV
Mb3567|M.bovis_AF2122/97 DPSNKPNPNLGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSII
Rv3537|M.tuberculosis_H37Rv DPSNKPNPNLGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSII
MMAR_4775|M.marinum_M DPTL-PNPNLYPLGDPPYYAFRIVLGDLGTSGGLRTDEYARVLRPDDTTV
MUL_0346|M.ulcerans_Agy99 DPTL-PNPNLYPLGDPPYYAFRIVLGDLGTSGGLRTDEYARVLRPDDTTV
MAB_0081|M.abscessus_ATCC_1997 DPTVTPNPNLRPLRRGPFYAVRMVLSDLGTCGGLRADERARVLREDGSVI
: : . : .* :
MSMEG_2867|M.smegmatis_MC2_155 PGLYAAGNTMAAVSG--TTYPGGGNPIGASMLFSHFAALDMAAEGTTA--
Mvan_2865|M.vanbaalenii_PYR-1 PGLYAAGNTMAAVSG--TTYPGGGNPIGASMLFSHLAALDMTGSPR----
Mflv_1547|M.gilvum_PYR-GCK PGLYAAGNTMAAPSG--TVYPGGGNPIGTSMVFSHLAVLDMVKGTTP---
MAV_4249|M.avium_104 PGVYAAGNTMAAPSG--TTYPGGGNPIGTSMLFSHLAVLDMRDRRP----
TH_3671|M.thermoresistible__bu RAMRASGMTTVTLIDCLTRDSAGCASVRAPYLTYRNAGTDQPVWHAAL--
Mb3567|M.bovis_AF2122/97 DGLYAAGNVSAPVMG--HTYPGPGGTIGPAMTFGYLAALHIADQAGKR--
Rv3537|M.tuberculosis_H37Rv DGLYAAGNVSAPVMG--HTYPGPGGTIGPAMTFGYLAALHIADQAGKR--
MMAR_4775|M.marinum_M GGLYAVGNTSAPVMG--RSYAGAGATIGPAMTFGFVAAKHLAAQLDARRA
MUL_0346|M.ulcerans_Agy99 GGLYAVGNTSAPVMG--RSYAGAGATIGPAMTFGFVAAKHLAARLDARRA
MAB_0081|M.abscessus_ATCC_1997 KGLYAIGNTAANAFG--ATYPGAGATIAQGLVYGYIAAHDAAAR------
.: * * . . . .. .: * .
MSMEG_2867|M.smegmatis_MC2_155 ---------
Mvan_2865|M.vanbaalenii_PYR-1 ---------
Mflv_1547|M.gilvum_PYR-GCK ---------
MAV_4249|M.avium_104 ---------
TH_3671|M.thermoresistible__bu ---------
Mb3567|M.bovis_AF2122/97 ---------
Rv3537|M.tuberculosis_H37Rv ---------
MMAR_4775|M.marinum_M TPTTHLHGR
MUL_0346|M.ulcerans_Agy99 TPTTHFHGR
MAB_0081|M.abscessus_ATCC_1997 ---------