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APLESPGEEPTTTWDEVTDVLVAGSGAGGVTGAYTAAREGLDVILVEATEKFGGTTAYSGGGGMWFPCNP VLMRAGVDDTIEDALEYYTSVVGDRTPRALQETYVRSGAPLVEYLEADELINFSLLPWPDYFGAAPKARA DGMRHIAAKPWKVAAAPHLRELVRGPLDSDRLGAPQPDDYFVGGRALIARLLAAAQRHPNSSARLNTALV ELVVEDGAVVGAVVDADGERRSIRTRRGVLLAAGGFEHNDDMRARYGVPGSSRDTMGPWGNRGLAHQAGI AVGADTDLMDQAWWSPGLTHPDGTSAFALWFTGGIFVDDHGRRFVNESAAYDRLGRAVLAAVDDGRVTLP FWMIYDSAGDRDGVVPPVKATNVSMVEPEKYIAAGLWRSADTLEELAVQIGVPPDNLVDSVARFNDFVRR GVDEDFGRGDEPYDRAFSGGVPPLYAIEKGPFHAAAFGVSDLGTKGGLRTDTAARVLDRADNVIPGLYAA GNTMAAPSGTVYPGGGNPIGTSMVFSHLAVLDMVKGTTP*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1547 | - | - | 100% (530) | 3-ketosteroid-delta-1-dehydrogenase |
| M. gilvum PYR-GCK | Mflv_1519 | - | 4e-77 | 37.70% (565) | 3-ketosteroid-delta-1-dehydrogenase |
| M. gilvum PYR-GCK | Mflv_4567 | - | 1e-68 | 34.29% (560) | 3-ketosteroid-delta-1-dehydrogenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3567 | - | 7e-72 | 35.97% (556) | 3-ketosteroid-delta-1-dehydrogenase |
| M. tuberculosis H37Rv | Rv3537 | - | 7e-72 | 35.97% (556) | 3-ketosteroid-delta-1-dehydrogenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0622c | - | 3e-78 | 37.25% (561) | 3-ketosteroid-delta-1-dehydrogenase |
| M. marinum M | MMAR_5024 | - | 3e-74 | 36.07% (560) | dehydrogenase |
| M. avium 104 | MAV_4249 | - | 0.0 | 73.68% (513) | 3-ketosteroid-delta-1-dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_2867 | - | 0.0 | 65.70% (516) | 3-ketosteroid-delta-1-dehydrogenase |
| M. thermoresistible (build 8) | TH_3671 | - | 1e-121 | 65.77% (336) | PUTATIVE fumarate reductase/succinate dehydrogenase |
| M. ulcerans Agy99 | MUL_4098 | - | 1e-74 | 36.07% (560) | 3-ketosteroid-delta-1-dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_5210 | - | 0.0 | 87.72% (513) | 3-ketosteroid-delta-1-dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
MMAR_5024|M.marinum_M ----------MFYMTSQDYDVVVVGSGGAGMVAALAAAHKGLSTVVIEKA
MUL_4098|M.ulcerans_Agy99 -------------MTSQDYDVVVVGSGGAGMVAALAAAHKGLSTVVIEKA
Mb3567|M.bovis_AF2122/97 -------------MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKA
Rv3537|M.tuberculosis_H37Rv -------------MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKA
MAB_0622c|M.abscessus_ATCC_199 -------------MTEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKA
Mflv_1547|M.gilvum_PYR-GCK MAPLESPGEEPTTTWDEVTDVLVAGSGAGGVTGAYTAAREGLDVILVEAT
Mvan_5210|M.vanbaalenii_PYR-1 MSPNE---------WDEVTDVLVAGSGAGGVTGAYTAAREGLEVILVEAT
MAV_4249|M.avium_104 MGTVQ---------WTAECDVLVAGSGAGGVTGAYTAAREGLEVLLVEAT
MSMEG_2867|M.smegmatis_MC2_155 MPDQRSE----SGRFDVEVDVLVAGSG-GGVAGAYTAAREGLSVLLVEAT
TH_3671|M.thermoresistible__bu MSD------------DIVVDVLVAGSG-GGLAGVYTAARAGLSVLLVEAT
**:*.*** .*:... .**: **..:::* :
MMAR_5024|M.marinum_M AHYGGSTARSGGG-VWIPNNQILKRARVSDTTEAARTYLHGIIGDSSVSG
MUL_4098|M.ulcerans_Agy99 AHYGGSTARSGGG-VWIPNNQILKRARVSDTTEAARTYLHGIIGDSSVSG
Mb3567|M.bovis_AF2122/97 PHYGGSTARSGGG-VWIPNNEVLKRRGVRDTPEAARTYLHGIVG----EI
Rv3537|M.tuberculosis_H37Rv PHYGGSTARSGGG-VWIPNNEVLKRRGVRDTPEAARTYLHGIVG----EI
MAB_0622c|M.abscessus_ATCC_199 PHFGGSTARSGGG-VWIPNNEVLKKARQTDTPEAAREYLYSIIG----DV
Mflv_1547|M.gilvum_PYR-GCK EKFGGTTAYSGGGGMWFPCNPVLMRAGVDDTIEDALEYYTSVVG----DR
Mvan_5210|M.vanbaalenii_PYR-1 DKFGGTTAYSGGGGMWFPCNPVLTRAGADDTLDDALEYYTAVVG----DR
MAV_4249|M.avium_104 SKFGGTTAYSGGGGVWFPCNPVLIRAGTDDTIEDALTYYHAVVG----DR
MSMEG_2867|M.smegmatis_MC2_155 DKFGGTTAFSGGGGMWFPCNPVLERAGTDDTLDEALKYFHAVVG----DR
TH_3671|M.thermoresistible__bu DKFGGTTAYSGGGGMWYPCNPVVRRAGTDDTIADALEYFHAVVG----DR
::**:** **** :* * * :: : ** * * .::* .
MMAR_5024|M.marinum_M VEPERIDTYLERGPEMLSFVLEHTPLKMCWVPGYSDYYPEAPGGRSGG-R
MUL_4098|M.ulcerans_Agy99 VEPERIDTYLERGPEMLSFVLEHTPLKMCWVPGYSDYYSEAPGGRSGG-R
Mb3567|M.bovis_AF2122/97 VEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGG-R
Rv3537|M.tuberculosis_H37Rv VEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGG-R
MAB_0622c|M.abscessus_ATCC_199 VPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYYPEAPGGRAAG-R
Mflv_1547|M.gilvum_PYR-GCK TPRALQETYVRSGAPLVEYLEADELINFSLLP-WPDYFGAAPKARADGMR
Mvan_5210|M.vanbaalenii_PYR-1 TPRALQETYVRSGAPLIEYLESDELLKFSLLP-WPDYFGKAPKARADGMR
MAV_4249|M.avium_104 TPRELQETYVRGGAPLIEYLEADPHLAFVPLP-WPDYYGKAPKARLDGQR
MSMEG_2867|M.smegmatis_MC2_155 TPQELQDAYVTGGAGFIAYLEQDHGFEFAVLP-WPDYYGSVPGARNDGYR
TH_3671|M.thermoresistible__bu TPRDLQDAYVTGGAGFIEFLEQDPHFEFTVLP-WPDYYGSAPGARNDGYR
. ::*: *. :: :: . : : :* :.**: .* .* * *
MMAR_5024|M.marinum_M SIEPKPFDARKLGADMAELEPAYGKIPLNVVVMQQDYVRLNQLKRHPRGV
MUL_4098|M.ulcerans_Agy99 SIEPKPFDARKLGADMAELEPAYGKIPLNVVVMQQDYVRLNQLKRHPRGV
Mb3567|M.bovis_AF2122/97 SIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRGV
Rv3537|M.tuberculosis_H37Rv SIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRGV
MAB_0622c|M.abscessus_ATCC_199 SVEPKPFDAKKLGDELPNLEPPYGKVPLNVVVRQQDYVRLNQLKRHPRGV
Mflv_1547|M.gilvum_PYR-GCK HIAAKPWKVAAAP----------------------------HLRELVRGP
Mvan_5210|M.vanbaalenii_PYR-1 HIAAKPLKVSAAP----------------------------ELRELVRGP
MAV_4249|M.avium_104 HIAAKPLPVAAAP----------------------------EFRTVIRGP
MSMEG_2867|M.smegmatis_MC2_155 HIVPKPLPDSALG----------------------------SYQGLVRGP
TH_3671|M.thermoresistible__bu HIVPVPLPDDALG----------------------------RHAGLVRGP
: . * **
MMAR_5024|M.marinum_M LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRK-GVPVQLNTALT
MUL_4098|M.ulcerans_Agy99 LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRK-GVPVQLNTALT
Mb3567|M.bovis_AF2122/97 LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRA-GVPVELNTAFT
Rv3537|M.tuberculosis_H37Rv LRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRA-GVPVELNTAFT
MAB_0622c|M.abscessus_ATCC_199 LRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDA-GVPVKLNTALT
Mflv_1547|M.gilvum_PYR-GCK LDSDRLGAPQPDDYFVG------GRALIARLLAAAQRHPNSSARLNTALV
Mvan_5210|M.vanbaalenii_PYR-1 LDSDRLGRPRPDDYFVG------GRALIARFLAAARRYPNSSARLNTALT
MAV_4249|M.avium_104 LDTDRLGAEPPSDYYLG------GRALIARFLRAIGEFPTATLRRDTALV
MSMEG_2867|M.smegmatis_MC2_155 LDTERLGAPAP-DTLIG------GRALVGRFLAALDKLPNADCWCEAPLT
TH_3671|M.thermoresistible__bu LDTERLGTPAP-DVLVG------GRALVGRFLAALGGFPDVTCWRNTPLT
* : ::* * **** . : . ::.:.
MMAR_5024|M.marinum_M DLYLEDGMVRGIYVRSTDAQESEEPQLIRARKGVILACGGFDHNEQMRVK
MUL_4098|M.ulcerans_Agy99 DLYLEDGVVRGIYVRSTDAHESEEPQLIRARKGVILACGGFDHNEQMRVK
Mb3567|M.bovis_AF2122/97 DLFVENGVVSGVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIK
Rv3537|M.tuberculosis_H37Rv DLFVENGVVSGVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIK
MAB_0622c|M.abscessus_ATCC_199 DLYIEDGTVRGVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVK
Mflv_1547|M.gilvum_PYR-GCK ELVVEDGAVVGAVVDADGERRS-----IRTRRGVLLAAGGFEHNDDMRAR
Mvan_5210|M.vanbaalenii_PYR-1 ELVVEDGRVVGAIVDTGGGRSA-----IRARRGVLLAAGGFEHNDAMRAR
MAV_4249|M.avium_104 ELVRTDGRVVGAVVETGGERRA-----VRARRGVLLAAGGFEANPDLRRA
MSMEG_2867|M.smegmatis_MC2_155 ELITESGRVVGAIVERGGERLR-----VCARRGVLLASGGFEQNADMRGR
TH_3671|M.thermoresistible__bu ELICDGDRVVGAVVVRDGTPVR-----VRARRGVLLAAGGFERNADMRAR
:* .. * * * : *:**:**.***: * :*
MMAR_5024|M.marinum_M YQRAPITTDWTVGAKANTGDGIVAAEKLGAALDLMEDAWWGP-TVPLVGR
MUL_4098|M.ulcerans_Agy99 YQRAPITTDWTVGAKANTGDGIVAAEKLGAALDLMEDAWWGP-TVPLVGR
Mb3567|M.bovis_AF2122/97 YQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWGP-TVPLVGK
Rv3537|M.tuberculosis_H37Rv YQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWGP-TVPLVGK
MAB_0622c|M.abscessus_ATCC_199 YQRAPITTEWTVGAKANTGDGILAAEKLGAALEFMEDSWWGP-TVPLVGR
Mflv_1547|M.gilvum_PYR-GCK YG-VPGSSRDTMGPWGNRGLAHQAGIAVGADTDLMDQAWWSPGLTHPDGT
Mvan_5210|M.vanbaalenii_PYR-1 YG-VPGASRDTMGPEGNQGLAHLAGMAVGADTDLMDQAWWSPGLTHPDGT
MAV_4249|M.avium_104 YG-VPGQARDTMGGPGSRGLALQAGIAVGADTDLLDQAWWSPGLTHPDGR
MSMEG_2867|M.smegmatis_MC2_155 YG-VPGSATDTMGGPGSTGAAHRAAMAVGADVDLMDQAWWSPGLTHPDGR
TH_3671|M.thermoresistible__bu YG-VPGDATDTMGAPGNTGAAHLAAIAAGADTDLMDQAWWSPGLTHPDGR
* .* : *:* .. * . *. ** ::::::**.* . *
MMAR_5024|M.marinum_M PWFALSERNSPGSIIVNMSGQRFMNESMPYVEACHHMYGGQYGQGPGPGE
MUL_4098|M.ulcerans_Agy99 PWFALSERNSPGSIIVNMSGQRFMNESMPYVEACHHMYGGQYGQGPGPGE
Mb3567|M.bovis_AF2122/97 PWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGE
Rv3537|M.tuberculosis_H37Rv PWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGE
MAB_0622c|M.abscessus_ATCC_199 PWFALSERNSPGSIIVNSSGKRFMNESLPYVEATHRMYGGQYGQGPGPGE
Mflv_1547|M.gilvum_PYR-GCK SAFALWFT---GGIFVDDHGRRFVNESAAYDRLGRAVLAAVDDGR----V
Mvan_5210|M.vanbaalenii_PYR-1 SAFALWFT---GGIFVDDHGRRFVNESAPYDRLGRAVLAAIADGT----V
MAV_4249|M.avium_104 SAFALWFT---GGIFVNQSADRFVNESMAYDRAGRQILAHLRDGS----L
MSMEG_2867|M.smegmatis_MC2_155 SAFALWFT---GGIFVDQDGKRFVNESAPYDRLGRAVIERLESGR----L
TH_3671|M.thermoresistible__bu SAFALWFT---GGIFVDQNGRRFVNESAPYDRLGREGXXXAGGHR----V
. *** *.*:*: . **:*** .* . : .
MMAR_5024|M.marinum_M NIPAWLVFD---QRYRDRYIFAGLQPGQRIPRKWLDSGVIVMADTLEELA
MUL_4098|M.ulcerans_Agy99 NIPAWLVFD---QRYRDRYIFAGLQPGQRIPRKWLDSGVIVMADTLEELA
Mb3567|M.bovis_AF2122/97 NIPAWLVFD---QRYRDRYIFAGLQPGQRIPSRWLDSGVIVQADTLAELA
Rv3537|M.tuberculosis_H37Rv NIPAWLVFD---QRYRDRYIFAGLQPGQRIPSRWLDSGVIVQADTLAELA
MAB_0622c|M.abscessus_ATCC_199 NLPAWLVFD---QEYRNRYIFAGLQPGQKIPSKWIESGVIVKADTIEELA
Mflv_1547|M.gilvum_PYR-GCK TLPFWMIYDSAGDRDGVVPPVKATNVSMVEPEKYIAAGLWRSADTLEELA
Mvan_5210|M.vanbaalenii_PYR-1 TLPFWMIYD---DSEGLVPPVKATNVSTVEPERYVSAGLWRTADTLEELA
MAV_4249|M.avium_104 RLPYWMIYD---DKEGAVPPVKATNVSLVETEKYVAAGLWHTADSLPELA
MSMEG_2867|M.smegmatis_MC2_155 TLPYWMVYD---SRAGDVPPVGATNVSMVDPAEYRAAGLWRSAETISGLA
TH_3671|M.thermoresistible__bu GEP----------GQRGAAAVHAEHLGRDLPVDHPAEELH--ADRAHQGS
* . . : . . : *: :
MMAR_5024|M.marinum_M AKAGLPVDELTSTVSRFNGFARSGVDEDYHRGESAYDRYYGDPTNKPNPN
MUL_4098|M.ulcerans_Agy99 AKAGLPVDELTSTVSRFNGFARSGVDEDYHRGESAYDRYYGDPTNKPNPN
Mb3567|M.bovis_AF2122/97 GKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPN
Rv3537|M.tuberculosis_H37Rv GKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPN
MAB_0622c|M.abscessus_ATCC_199 KKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPN
Mflv_1547|M.gilvum_PYR-GCK VQIGVPPDNLVDSVARFNDFVRRGVDEDFGRGDEPYDRAFSGG----VPP
Mvan_5210|M.vanbaalenii_PYR-1 VAIGVPPQNLVETVARFNGFTRTGVDEDFGRGDEPYDRAFSGG----AAP
MAV_4249|M.avium_104 AKIGVPAQRLTATVLRFNGFAAAGVDEDFGRGDEAFDRAFSAG----ASP
MSMEG_2867|M.smegmatis_MC2_155 EEIGVPADALEATIQRFNEMATAGHDDDFGRGDEAYDRVFTGG----ASP
TH_3671|M.thermoresistible__bu GERIVDHGILGAARQRAG-------GRHHGRGG-----ADAGG----QVP
: * : * . . .. **
MMAR_5024|M.marinum_M LGEINHAPYYAAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNV
MUL_4098|M.ulcerans_Agy99 LGEINHAPYYAAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNV
Mb3567|M.bovis_AF2122/97 LGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNV
Rv3537|M.tuberculosis_H37Rv LGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNV
MAB_0622c|M.abscessus_ATCC_199 LGALTKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNV
Mflv_1547|M.gilvum_PYR-GCK LYAIEKGPFHAAAFGVSDLGTKGGLRTDTAARVLDRADNVIPGLYAAGNT
Mvan_5210|M.vanbaalenii_PYR-1 LYPIAKGPFHAAAFGVSDLGTKGGLRTDTAARVLDRADNVIPGLYAAGNT
MAV_4249|M.avium_104 LVPIDTPPYHAAAFGVSDLGTKGGLRTDTAARVLDTAGRPIPGVYAAGNT
MSMEG_2867|M.smegmatis_MC2_155 LVPIDTPPYHAAAFGLSDLGTKGGLRTDTRARVRGRDGEPIPGLYAAGNT
TH_3671|M.thermoresistible__bu GVVVGTRHRRPLTDQCTNPELVS--CHNSSSRLYARFRGPVRAMRASGMT
: : . : .* : .: *:* .
MMAR_5024|M.marinum_M SAPVMG--HTYPGPGGTIGPAMTFGYLAALHIAGDR---
MUL_4098|M.ulcerans_Agy99 SAPVMG--HTYPGPGGTIGPAMTFGYLAALHIAGDR---
Mb3567|M.bovis_AF2122/97 SAPVMG--HTYPGPGGTIGPAMTFGYLAALHIADQAGKR
Rv3537|M.tuberculosis_H37Rv SAPVMG--HTYPGPGGTIGPAMTFGYLAALHIADQAGKR
MAB_0622c|M.abscessus_ATCC_199 STPVMG--HTYAGPGATIGPAMTWGYLAALDIAGK----
Mflv_1547|M.gilvum_PYR-GCK MAAPSG--TVYPGGGNPIGTSMVFSHLAVLDMVKGTTP-
Mvan_5210|M.vanbaalenii_PYR-1 MAAPSG--TVYPGGGNPIGTSMVFSHLAVLDMAGGHS--
MAV_4249|M.avium_104 MAAPSG--TTYPGGGNPIGTSMLFSHLAVLDMRDRRP--
MSMEG_2867|M.smegmatis_MC2_155 MAAVSG--TTYPGGGNPIGASMLFSHFAALDMAAEGTTA
TH_3671|M.thermoresistible__bu TVTLIDCLTRDSAGCASVRAPYLTYRNAGTDQPVWHAAL
.. . .. .: .. * .