For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. smegmatis MC2 155 MSMEG_2496 (-)

annotation: NAD-binding protein
coordinates: 2581723 - 2582772
length: 349

LVPTLDRVPTPNQSGAAGGRAVRDRRPAVAVLCAQESDRPPGLEALDVEFRYCTADTLAEAVRGARAMLL
WDFFSKAVRDVWSAAGSVEWIHVTAAGVDTLLFDELRDSDVVVTNAHGVFDRPIAEYVLGAVIAHAKDTR
RSLDLQRQHRWQHRETRRVTGSRALVVGTGGIGRETARLLRAAGLEVRGAGRTARDDDPDFGRVVASSDL
SAEVGWCDHLILAVPLTDATRGLVDAAVLGAMKPDAHLVNIARGPVVDEAALIAALTEHRIGGATLDAFD
VEPLPADHPLWDVPNVTITAHMSGDVVGWRDTLAEQYAENVRRWLAGEPLHNVVDKKLGYISQAGRGVR

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. smegmatis MC2 155 MSMEG_2496 - - 100% (349) NAD-binding protein
M. smegmatis MC2 155 MSMEG_3422 - 2e-36 38.34% (253) D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding
M. smegmatis MC2 155 MSMEG_0604 - 4e-31 33.65% (312) glyoxylate reductase

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3020c serA1 1e-21 29.29% (297) D-3-phosphoglycerate dehydrogenase
M. gilvum PYR-GCK Mflv_4768 - 7e-28 36.04% (222) D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding
M. tuberculosis H37Rv Rv2996c serA1 1e-21 29.29% (297) D-3-phosphoglycerate dehydrogenase
M. leprae Br4923 MLBr_01692 serA 2e-20 28.73% (268) D-3-phosphoglycerate dehydrogenase
M. abscessus ATCC 19977 MAB_4414 - 1e-71 45.86% (314) putative NAD-binding protein (D-isomer specific 2-hydroxyacid
M. marinum M MMAR_5282 serA4 3e-27 33.33% (249) D-3-phosphoglycerate dehydrogenase SerA4
M. avium 104 MAV_0349 - 7e-29 40.00% (205) D-isomer specific 2-hydroxyacid dehydrogenase family protein
M. thermoresistible (build 8) TH_3383 - 1e-26 31.69% (325) PUTATIVE D-isomer specific 2-hydroxyacid dehydrogenase,
M. ulcerans Agy99 MUL_4355 serA4 4e-27 33.33% (249) D-3-phosphoglycerate dehydrogenase SerA4
M. vanbaalenii PYR-1 Mvan_0404 - 5e-29 33.44% (302) D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

CLUSTAL 2.0.9 multiple sequence alignment


TH_3383|M.thermoresistible__bu      VDRPAVALSANRRRHCPGMLRGDMSGVNSRSALRVLAHFRPG---PKVLE
Mvan_0404|M.vanbaalenii_PYR-1       --------------------------------MRVLAHFVPGGPDSKVIE
Mb3020c|M.bovis_AF2122/97           ----------------------------------MSLPVVLIADKLAPST
Rv2996c|M.tuberculosis_H37Rv        ----------------------------------MSLPVVLIADKLAPST
MLBr_01692|M.leprae_Br4923          ----------------------------------MDLPVVLIADKLAQST
Mflv_4768|M.gilvum_PYR-GCK          ----------------------------------MQVELPRHRKRIEDLG
MMAR_5282|M.marinum_M               ----------------MSQPASHGFGQDHVPRVAILDDYARVATDLADWS
MUL_4355|M.ulcerans_Agy99           ----------------MSQPASHGFGQDHVPRVAILDDYARVATDLADWS
MAV_0349|M.avium_104                -----------------------------MPRLAILDDYARVALQVADWS
MSMEG_2496|M.smegmatis_MC2_155      ----------MVPTLDRVPTPNQSGAAGGRAVRDRRPAVAVLCAQESDRP
MAB_4414|M.abscessus_ATCC_1997      --------------------------MNEQLVR--RPVIAVQHSPDS-VP
                                                                                      

TH_3383|M.thermoresistible__bu      FLEPESGWLDVRFCDEDDDETFHRELPHAEVIWHVLRPLSGEDLTRATRC
Mvan_0404|M.vanbaalenii_PYR-1       FLAPCRDWLDVRFCAEDDDDTFYRELADAEVIWHVLRPLSGEDLARAPRL
Mb3020c|M.bovis_AF2122/97           VAALGDQVEVRWVDGPDRDKLLAAVPEADALLVRSATTVDAEVLAAAPKL
Rv2996c|M.tuberculosis_H37Rv        VAALGDQVEVRWVDGPDRDKLLAAVPEADALLVRSATTVDAEVLAAAPKL
MLBr_01692|M.leprae_Br4923          VAALGDQVEVRWVDGPDRTKLLAAVPEADALLVRSATTVDAEVLAAAPKL
Mflv_4768|M.gilvum_PYR-GCK          FEVLAPELGGRQQFSSSE---LLEYSSRLIGIIAGDDELDADFFAGAGKL
MMAR_5282|M.marinum_M               PVRGRATLTVFDRHLGQAEAAEALADFDAVCTMRERMAFPRSLLERLPRL
MUL_4355|M.ulcerans_Agy99           PVRGRATLTVFDRHLGQAEAAEALADFDAVCTMRERMAFPRSLLERLPRL
MAV_0349|M.avium_104                RVQKRCDITVFDEHLSEQQAVDALRPFDVVCTMRERMAFPRSLIERLPHL
MSMEG_2496|M.smegmatis_MC2_155      PGLEALD--VEFRYCTADTLAEAVRGARAMLLWDFFSKAVRDVWSAAGSV
MAB_4414|M.abscessus_ATCC_1997      ERLAPVTAAAEVRLVESDRLATALPGAEVLFVYDFRSSALRETWSAADSL
                                                                             .        

TH_3383|M.thermoresistible__bu      RLVHKLGTGVNTIDVDTATERGIAVANMPGAN------AASVAEGTVMLM
Mvan_0404|M.vanbaalenii_PYR-1       RLVHKLGAGVNTIDVDAATARGVAVANMPGAN------AESVAEGTLLLM
Mb3020c|M.bovis_AF2122/97           KIVARAGVGLDNVDVDAATARGVLVVNAPTSN------IHSAAEHALALL
Rv2996c|M.tuberculosis_H37Rv        KIVARAGVGLDNVDVDAATARGVLVVNAPTSN------IHSAAEHALALL
MLBr_01692|M.leprae_Br4923          KIVARAGVGLDNVDVDAATARGVLVVNAPTSN------IHSAAEHALALL
Mflv_4768|M.gilvum_PYR-GCK          KTVIRWGIGMDSVDHEAARRHGVTVRNTPGVF------GYEVADSAFGYI
MMAR_5282|M.marinum_M               KLITIVGMNLPNLDLAAATERGVLVAHSNFAHPRFRAARDATAEFAWGLL
MUL_4355|M.ulcerans_Agy99           KLITIVGMNLPNLDLAAATERGVLVAHSNFAHPRFRAARDATAEFAWGLL
MAV_0349|M.avium_104                RLLTIVGRQLPNLDMAAATEHGIAVARSEFAHPRFAALRDATPELAWGLM
MSMEG_2496|M.smegmatis_MC2_155      EWIHVTAAGVDTLLFDELRDSDVVVTNAHGVF------DRPIAEYVLGAV
MAB_4414|M.abscessus_ATCC_1997      RWVQIASTGVDPVLFDEFVASDVVLTNSRGIF------ERPIAEYVLAQI
                                    . :   .  :  :        .: : .               .: .   :

TH_3383|M.thermoresistible__bu      LAALRRLVELDRATRAGTGWPTDPSLGETVRDIGGSTVGLIGYGNVARRV
Mvan_0404|M.vanbaalenii_PYR-1       LAALRRLPVLDRATREGRGWPSDPTLGETVRDIGGCTVGLVGYGNIAKQV
Mb3020c|M.bovis_AF2122/97           LAASRQIPAADASLREH-TWKRSSFSGTEIF---GKTVGVVGLGRIGQLV
Rv2996c|M.tuberculosis_H37Rv        LAASRQIPAADASLREH-TWKRSSFSGTEIF---GKTVGVVGLGRIGQLV
MLBr_01692|M.leprae_Br4923          LAASRQIAEADASLRAH-IWKRSSFSGTEIF---GKTVGVVGLGRIGQLV
Mflv_4768|M.gilvum_PYR-GCK          LNLARGYMAVDAAVRRG-EWP--KVEGITLD---GSRLGIVGFGAIGREI
MMAR_5282|M.marinum_M               IATVRNLAEEHRQLRAG-GWQT--TTGMTLS---GKTLGLLGLGRVGKRM
MUL_4355|M.ulcerans_Agy99           IATVHNLAEEHRQVRAG-GWQT--TTGMTLS---GKTLGLLGLGRVGKRM
MAV_0349|M.avium_104                IATVRHLADEHRQMRAG-GWQR--TAGMTLS---GKTLGLLGLGRVGSRM
MSMEG_2496|M.smegmatis_MC2_155      IAHAKDTRRSLDLQRQH-RWQHRETRRVTGS-----RALVVGTGGIGRET
MAB_4414|M.abscessus_ATCC_1997      LAFAKDIRRSTRAQAAL-SWRHFESESIEGT-----PVGVLGTGPIGQAI
                                    :   :              *                   ::* * :.   

TH_3383|M.thermoresistible__bu      EQIVAAMGAEVLHTS------TADDGH-PNWRPLNDLLARSDIVSIHVPL
Mvan_0404|M.vanbaalenii_PYR-1       ERILVAMGADVIHTS------TRDDGS-AGWRSLPDLLSACDIVSLHLPL
Mb3020c|M.bovis_AF2122/97           AQRIAAFGAYVVAYDPYVSPARAAQLG-IELLSLDDLLARADFISVHLPK
Rv2996c|M.tuberculosis_H37Rv        AQRIAAFGAYVVAYDPYVSPARAAQLG-IELLSLDDLLARADFISVHLPK
MLBr_01692|M.leprae_Br4923          AARIAAFGAHVIAYDPYVAPARAAQLG-IELMSFDDLLARADFISVHLPK
Mflv_4768|M.gilvum_PYR-GCK          AKRGAGFGQEVVAFDPFVK---ASPAG-VSMVELDELLATSRFVVLACPL
MMAR_5282|M.marinum_M               TEYAKAFGMDVIAWSQNLTEEAAAAHG-VRRVDKPVLFAQSDVVSIHVVL
MUL_4355|M.ulcerans_Agy99           TEYAKAFGMDVIAWSQNLTEEAAAAHG-VRRVDKPVLFAQSDMVSIHVVL
MAV_0349|M.avium_104                AGFAAAFGMEVIAWSQNLTDDAAAAVG-ARRVSKAALFESADVVSVHLVL
MSMEG_2496|M.smegmatis_MC2_155      ARLLRAAGLEVRGAGRTARDDDPDFGRVVASSDLSAEVGWCDHLILAVPL
MAB_4414|M.abscessus_ATCC_1997      STLLGAVGMRVTVLGR------------AESADLSSHVGEFEYLVLAAPL
                                         . *  *   .                      .     : :    

TH_3383|M.thermoresistible__bu      TEKTLGLLDATALSRMKPAAVLVNTARGAIVDEIALTEALHARRLAAAGL
Mvan_0404|M.vanbaalenii_PYR-1       TDATAGLLGRDALARMKSDAVLVNTSRGPIVDEEALADALRTGKLAAAGL
Mb3020c|M.bovis_AF2122/97           TPETAGLIDKEALAKTKPGVIIVNAARGGLVDEAALADAITGGHVRAAGL
Rv2996c|M.tuberculosis_H37Rv        TPETAGLIDKEALAKTKPGVIIVNAARGGLVDEAALADAITGGHVRAAGL
MLBr_01692|M.leprae_Br4923          TPETAGLIDKEALAKTKPGVIIVNAARGGLVDEVALADAVRSGHVRAAGL
Mflv_4768|M.gilvum_PYR-GCK          TPETFHLIDADRLAGMRSDSFLVNVARGPVVLEAALIDALKAGRLAGAAL
MMAR_5282|M.marinum_M               SERTRALVGAGELAAMKPGAYLINTSRGPIVDEGALIGALEAGRIAGAGL
MUL_4355|M.ulcerans_Agy99           SERTRALVGAGELAAMKPGAYLINTSRGPIVDEGALIGALEAGRIAGAGL
MAV_0349|M.avium_104                SERTRGLVGEPELALMKPSAYLINTARGPIVDEPALIAALTAGRIAGAGL
MSMEG_2496|M.smegmatis_MC2_155      TDATRGLVDAAVLGAMKPDAHLVNIARGPVVDEAALIAALTEHRIGGATL
MAB_4414|M.abscessus_ATCC_1997      TPQTRGIVNARVLSAMRPTARLINVGRGELVGTWDLVSALNRGGIAGAAL
                                    :  *  ::.   *.  :.   ::* .** :*    *  *:    : .* *

TH_3383|M.thermoresistible__bu      DVFATEPVRPNNPLLTLDNVVLTPHVSWYTVDTMRRYLTHAVDNCRRLRD
Mvan_0404|M.vanbaalenii_PYR-1       DVFAVEPVPADNPLLRLPNVVLTPHVTWYTADTMRRYLEFAVDNCERLRD
Mb3020c|M.bovis_AF2122/97           DVFATEPC-TDSPLFELAQVVVTPHLGASTAEAQDRAGTDVAESVRLALA
Rv2996c|M.tuberculosis_H37Rv        DVFATEPC-TDSPLFELAQVVVTPHLGASTAEAQDRAGTDVAESVRLALA
MLBr_01692|M.leprae_Br4923          DVFATEPC-TDSPLFELSQVVVTPHLGASTAEAQDRAGTDVAESVRLALA
Mflv_4768|M.gilvum_PYR-GCK          DVFEVEPLPLDSELRTLPNVVLGAHNGSNTREGVVRASNAAVEFLIEELA
MMAR_5282|M.marinum_M               DVYDVEPLPVDHRLRSLPNVTLSPHLGYVTREMLSACYADTVEAVVAWLD
MUL_4355|M.ulcerans_Agy99           DVYDVEPLPVDHRLRSLPNVTLSPHLGYVTREMLSVCYADTVEAVVAWLD
MAV_0349|M.avium_104                DVYDVEPLPGDHPLRSLPNVTLSPHLGYVTREMLGAFYADTVESVLAWLD
MSMEG_2496|M.smegmatis_MC2_155      DAFDVEPLPADHPLWDVPNVTITAHMSGDVVGWRDTLAEQYAENVRRWLA
MAB_4414|M.abscessus_ATCC_1997      DVTDPEPLPVGHPLWRTPNTHITPHNSGDVRGWSDRLQDQFVVNFERYLR
                                    *.   **   .  *    :. : .*    .           .        

TH_3383|M.thermoresistible__bu      GEQLADVVNGITGRRPG---------------------------------
Mvan_0404|M.vanbaalenii_PYR-1       GRNLVNVVNDVAG-------------------------------------
Mb3020c|M.bovis_AF2122/97           GEFVPDAVNVGGGVVNEEVAPWLDLVRKLGVLAGVLSDELPVSLSVQVRG
Rv2996c|M.tuberculosis_H37Rv        GEFVPDAVNVGGGVVNEEVAPWLDLVRKLGVLAGVLSDELPVSLSVQVRG
MLBr_01692|M.leprae_Br4923          GEFVPDAVNVDGGVVNEEVAPWLDLVCKLGVLVAALSDELPASLSVHVRG
Mflv_4768|M.gilvum_PYR-GCK          R-------------------------------------------------
MMAR_5282|M.marinum_M               GAPIRIANPQAVQRPPS---------------------------------
MUL_4355|M.ulcerans_Agy99           GAPIRIANPQAVRRPPS---------------------------------
MAV_0349|M.avium_104                GTPIRIANPEVLQRD-----------------------------------
MSMEG_2496|M.smegmatis_MC2_155      GEPLHNVVDKKLGYISQAGRGVR---------------------------
MAB_4414|M.abscessus_ATCC_1997      GEELLNIVDKNRMHRHA---------------------------------
                                                                                      

TH_3383|M.thermoresistible__bu      --------------------------------------------------
Mvan_0404|M.vanbaalenii_PYR-1       --------------------------------------------------
Mb3020c|M.bovis_AF2122/97           ELAAEEVEVLRLSALRGLFSAVIEDAVTFVNAPALAAERGVTAEICKASE
Rv2996c|M.tuberculosis_H37Rv        ELAAEEVEVLRLSALRGLFSAVIEDAVTFVNAPALAAERGVTAEICKASE
MLBr_01692|M.leprae_Br4923          ELASEDVEILRLSALRGLFSTVIEDAVTFVNAPALAAERGVSAEITTGSE
Mflv_4768|M.gilvum_PYR-GCK          --------------------------------------------------
MMAR_5282|M.marinum_M               --------------------------------------------------
MUL_4355|M.ulcerans_Agy99           --------------------------------------------------
MAV_0349|M.avium_104                --------------------------------------------------
MSMEG_2496|M.smegmatis_MC2_155      --------------------------------------------------
MAB_4414|M.abscessus_ATCC_1997      --------------------------------------------------
                                                                                      

TH_3383|M.thermoresistible__bu      --------------------------------------------------
Mvan_0404|M.vanbaalenii_PYR-1       --------------------------------------------------
Mb3020c|M.bovis_AF2122/97           SPNHRSVVDVRAVGADGSVVTVSGTLYGPQLSQKIVQINGRHFDLRAQGI
Rv2996c|M.tuberculosis_H37Rv        SPNHRSVVDVRAVGADGSVVTVSGTLYGPQLSQKIVQINGRHFDLRAQGI
MLBr_01692|M.leprae_Br4923          SPNHRSVVDVRAVASDGSVVNIAGTLSGPQLVQKIVQVNGRNFDLRAQGM
Mflv_4768|M.gilvum_PYR-GCK          --------------------------------------------------
MMAR_5282|M.marinum_M               --------------------------------------------------
MUL_4355|M.ulcerans_Agy99           --------------------------------------------------
MAV_0349|M.avium_104                --------------------------------------------------
MSMEG_2496|M.smegmatis_MC2_155      --------------------------------------------------
MAB_4414|M.abscessus_ATCC_1997      --------------------------------------------------
                                                                                      

TH_3383|M.thermoresistible__bu      --------------------------------------------------
Mvan_0404|M.vanbaalenii_PYR-1       --------------------------------------------------
Mb3020c|M.bovis_AF2122/97           NLIIHYVDRPGALGKIGTLLGTAGVNIQAAQLSEDAEGPGATILLRLDQD
Rv2996c|M.tuberculosis_H37Rv        NLIIHYVDRPGALGKIGTLLGTAGVNIQAAQLSEDAEGPGATILLRLDQD
MLBr_01692|M.leprae_Br4923          NLVIRYVDQPGALGKIGTLLGAAGVNIQAAQLSEDTEGPGATILLRLDQD
Mflv_4768|M.gilvum_PYR-GCK          --------------------------------------------------
MMAR_5282|M.marinum_M               --------------------------------------------------
MUL_4355|M.ulcerans_Agy99           --------------------------------------------------
MAV_0349|M.avium_104                --------------------------------------------------
MSMEG_2496|M.smegmatis_MC2_155      --------------------------------------------------
MAB_4414|M.abscessus_ATCC_1997      --------------------------------------------------
                                                                                      

TH_3383|M.thermoresistible__bu      ------------------------
Mvan_0404|M.vanbaalenii_PYR-1       ------------------------
Mb3020c|M.bovis_AF2122/97           VPDDVRTAIAAAVDAYKLEVVDLS
Rv2996c|M.tuberculosis_H37Rv        VPDDVRTAIAAAVDAYKLEVVDLS
MLBr_01692|M.leprae_Br4923          VPGDVRSAIVAAVSANKLEVVNLS
Mflv_4768|M.gilvum_PYR-GCK          ------------------------
MMAR_5282|M.marinum_M               ------------------------
MUL_4355|M.ulcerans_Agy99           ------------------------
MAV_0349|M.avium_104                ------------------------
MSMEG_2496|M.smegmatis_MC2_155      ------------------------
MAB_4414|M.abscessus_ATCC_1997      ------------------------

species	id	gene	e-value	identity (len)	annotation
M. smegmatis MC2 155	MSMEG_2496	-	-	100% (349)	NAD-binding protein
M. smegmatis MC2 155	MSMEG_3422	-	2e-36	38.34% (253)	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding
M. smegmatis MC2 155	MSMEG_0604	-	4e-31	33.65% (312)	glyoxylate reductase