For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. smegmatis MC2 155 MSMEG_1897 (pcaD)

annotation: 3-oxoadipate enol-lactonase
coordinates: 1976738 - 1977514
length: 258

MSSVAVHHVVTGRSDGPAVVFSNSLGSTHRMWDPQLAAFEERFRVVRYDTRGHGQSPVPEGPYTIDDLAD
DVVALLDTLGIERAHVVGLSLGGMTAMRLSARNPQRVDRIALLCTATHLPPSSAWTERAAAVRTHGSAAV
AESVVGRWFTPGFLAADPGRKAAYQQMIAATPAEGYAGCCEAIAALDLRADLARITAPTLAIAGTDDPAT
PPDRLAEITAAVRGARLVQVTPGAHLANVEQADAITPTLLEHLGTEQS
Operon Prediction Model: Genebank

Paralogs
speciesidgenee-valueidentity (len)annotation
M. smegmatis MC2 155MSMEG_1897pcaD-100% (258)3-oxoadipate enol-lactonase
M. smegmatis MC2 155MSMEG_4707-9e-2028.15% (270) non-heme bromoperoxidase BPO-A2
M. smegmatis MC2 155MSMEG_1352-5e-1828.46% (260) hydrolase, alpha/beta fold family protein

Closest Orthologs (e-value cutoff: 1e-4)
speciesidgenee-valueidentity (len)annotation
M. bovis AF2122 / 97Mb3196chpx3e-1528.26% (276) non-Heme haloperoxidase Hpx
M. gilvum PYR-GCKMflv_1348-7e-9566.01% (253) 3-oxoadipate enol-lactonase
M. tuberculosis H37RvRv3171chpx5e-1628.62% (276) non-Heme haloperoxidase Hpx
M. leprae Br4923MLBr_01899lipG1e-1630.50% (259) putative hydrolase
M. abscessus ATCC 19977MAB_0349c-3e-2030.04% (253) putative hydrolase, alpha/beta fold
M. marinum MMMAR_1388hpx4e-1728.21% (280) non-heme haloperoxidase Hpx
M. avium 104MAV_4589-7e-1728.02% (257) hydrolase, alpha/beta fold family protein
M. thermoresistible (build 8)TH_2963-2e-2732.41% (253) PUTATIVE putative 3-oxoadipate enol-lactonase
M. ulcerans Agy99MUL_2488hpx3e-1728.21% (280) non-heme haloperoxidase Hpx
M. vanbaalenii PYR-1Mvan_0563-8e-3935.83% (254) 4-carboxymuconolactone decarboxylase

CLUSTAL 2.0.9 multiple sequence alignment


MSMEG_1897|M.smegmatis_MC2_155      ---------------------------------MSSVAVHHVVTGRSD-G
Mflv_1348|M.gilvum_PYR-GCK          ---------------------------------MSAVDVHVIESGLPD-G
Mvan_0563|M.vanbaalenii_PYR-1       ---------------------------------MSNPPLTAIHLGGPDDG
Mb3196c|M.bovis_AF2122/97           ----------------------------MTVRAADGTPLHTQVFGPPHGY
Rv3171c|M.tuberculosis_H37Rv        ----------------------------MTVRAADGTPLHTQVFGPPHGY
MMAR_1388|M.marinum_M               MSARKATDGKAHTKAPAALPDVLPPSRSLTVRAADGTALHTQVFGPADGY
MUL_2488|M.ulcerans_Agy99           MSARKATDGKAHTKASAALPDVLPPSRSLTVRAADGTALHTQVFGPADGY
MAB_0349c|M.abscessus_ATCC_199      ------------------------MGSAALQDRYLQSCLGGLHVRIGGEG
MAV_4589|M.avium_104                -------------------------------------MINLAYDDRGSGE
TH_2963|M.thermoresistible__bu      ----------------------------MPRFSRAGTTIDYTDTGAPAGR
MLBr_01899|M.leprae_Br4923          ------------------------MEVRSGYATSGDLKLYYEDMGNVDDP
                                                                          :           

MSMEG_1897|M.smegmatis_MC2_155      PAVVFSNSLG-STHRMWD--PQLAAFEERFRVVRYDTRGHGQSPVP----
Mflv_1348|M.gilvum_PYR-GCK          PAVVLSNSLG-ATHRMWD--AQLDALERRFRVLRYDTRGHGASPVP----
Mvan_0563|M.vanbaalenii_PYR-1       PLLLLGPSLGTTTATLWT--GVAQRLVDHVRVVGWDLPGHGRGRR-----
Mb3196c|M.bovis_AF2122/97           PIVLTHGFVC--AIRAWA--YQIADLAGDYRVIAFDHRGHGRSGVPR---
Rv3171c|M.tuberculosis_H37Rv        PIVLTHGFVC--AIRAWA--YQIADLAGDYRVIAFDHRGHGRSGVPR---
MMAR_1388|M.marinum_M               PIVLTHGFVC--SIRAWA--YQIADLATDYRVIAFDHRGHGRSGIPR---
MUL_2488|M.ulcerans_Agy99           PIVLTHGFVC--SIRAWA--YQIADLATDYRVIAFDHRGHGRSGIPR---
MAB_0349c|M.abscessus_ATCC_199      PVILFWPSLL-MTGSMWA--AQAEYFADRYTVILVDPPGHG-DSQSL---
MAV_4589|M.avium_104                PVVFIAGHGG--AGRTWHPYQVPAFLAAGYRVITFDNRGIGATEN-----
TH_2963|M.thermoresistible__bu      LEAPTILFGHGLLFSGWQFNLQIYALRRYYRCLAIDWRGQGASSG-A---
MLBr_01899|M.leprae_Br4923          PVLLIMGLGA--QLVLWRTAFCEKLVAQGLRVVRYDNRDVGLSSRTDSTE
                                                    *        .      :  *  . *         

MSMEG_1897|M.smegmatis_MC2_155      ---------------------EGPYTIDDLADDVVALLDT--LGIERAHV
Mflv_1348|M.gilvum_PYR-GCK          ---------------------DGPYSIDDLADDVVGILDR--RGIDTAHL
Mvan_0563|M.vanbaalenii_PYR-1       ---------------------AHPFTIADLAAAVLVIADD--LNVETFHY
Mb3196c|M.bovis_AF2122/97           ---------------------RGAYSLNHLAADLDSVLDATLAPRERAVV
Rv3171c|M.tuberculosis_H37Rv        ---------------------RGAYSLNHLAADLDSVLDATLAPRERAVV
MMAR_1388|M.marinum_M               ---------------------RGGYTLEHLASDLDSVLDATLAPHERALI
MUL_2488|M.ulcerans_Agy99           ---------------------RGGYTLEHLASDLDSVLDATLAPHERALI
MAB_0349c|M.abscessus_ATCC_199      ---------------------TRMFRFEDCAHAITQILDS--LGIERAHV
MAV_4589|M.avium_104                ---------------------AQGFTTETMVADTAALIEG--LNAAPARI
TH_2963|M.thermoresistible__bu      ---------------------ARPYDMDTLTEDAAHLVRS--LGVAPAHY
MLBr_01899|M.leprae_Br4923          QPCPSQPLTARLIRFWLGQRNACAYTLEDMTDDAVALLDH--LSIERAHI
                                                            :     .     :             

MSMEG_1897|M.smegmatis_MC2_155      VGLSLGGMTAMRLSARNPQRVDR------------------IALLCTATH
Mflv_1348|M.gilvum_PYR-GCK          VGLSLGGMTAMRVAARNPDRVNR------------------MVLLCTAAE
Mvan_0563|M.vanbaalenii_PYR-1       AGDSVGGCVGLQLLLDAPQRVSS------------------ATLLCTGAA
Mb3196c|M.bovis_AF2122/97           AGHSMGGITIAAWSDRYRHKVRRRTDAVALINTTTGDLVRKVKLLSVPRE
Rv3171c|M.tuberculosis_H37Rv        AGHSMGGITIAAWSDRYRHKVRRRTDAVALINTTTGDLVRKVKLLSVPRE
MMAR_1388|M.marinum_M               AGHSMGGIAISAWSERYRHKVHRRADAVALINTATGDLLRKIRMLSVPRE
MUL_2488|M.ulcerans_Agy99           AGHSMGGIAISAWSERYRHKVHRRADAVALINTATGDLLRKIRMLSVPRE
MAB_0349c|M.abscessus_ATCC_199      VGNSWGGMIGGTFTAMYPHRAGAS---------------VLMNATASRAS
MAV_4589|M.avium_104                VGMSMGAFIAQELMLARPELVSA------------------AVLMGTRGR
TH_2963|M.thermoresistible__bu      VGLSMGGFVGIRLAARHPELVRS---------------LVLMNTSAGPED
MLBr_01899|M.leprae_Br4923          VGASMGGMIAQIFAARFPTRTRS------------------LAVFFSSNN
                                    .* * *.             .                             

MSMEG_1897|M.smegmatis_MC2_155      LPPSSAWTERAAAVRTHG--SAAVAESVVGRWFTPGFLAA----DPGRKA
Mflv_1348|M.gilvum_PYR-GCK          LAPAQAWTDRAAAVRAGG--AESVAPAVIARWFTLSHLET----HPAERA
Mvan_0563|M.vanbaalenii_PYR-1       IGTPDGWLARAATVRAGG--VDTMLAGAAERWFAPGFVDR----EPGTAS
Mb3196c|M.bovis_AF2122/97           LSPVRVLAGRSLVNTFGG--FPLPGAARALSRHVISTLAVAADADPSATR
Rv3171c|M.tuberculosis_H37Rv        LSPVRVLAGRSLVNTFGG--FPLPGAARALSRHVISTLAVAADADPSATR
MMAR_1388|M.marinum_M               LSAARDFAGRGLINAFGG--FPLPSAVRIPTQYLIAMMAVGADAHPTAAR
MUL_2488|M.ulcerans_Agy99           LSAARDFAGRGLINAFGG--FPLPSAVRIPTQYLIAMMAVGADAHPTAAR
MAB_0349c|M.abscessus_ATCC_199      MRQRVEFRLLTEIVRILGR-FREPLTTRAVKAFVGPTIMR---ERPHVEQ
MAV_4589|M.avium_104                MDRARQFFRDAEAELADG--GVALPASYEAKIRLLENFSRKTLNDDTAVA
TH_2963|M.thermoresistible__bu      PAKADRYRTMARIYRFTG--IGPLRRAVLPLMFGPAFLADP--DSEDVID
MLBr_01899|M.leprae_Br4923          RPFLPPPAPRALLALLTGPPTGSRRDVVVDNVVRVTKITGSPLYRMPEEQ
                                                     *                   .            

MSMEG_1897|M.smegmatis_MC2_155      AYQQMIAATPAEGYAG--CCEAIAALD--LRADLAR-ITAPTLAIAGTDD
Mflv_1348|M.gilvum_PYR-GCK          EWVDMIAATPAEGYAS--CCEAIAELD--LREQLPT-ITAPTLAIAGADD
Mvan_0563|M.vanbaalenii_PYR-1       ALLDALSHTDAESYAQ--VCEALAVFD--VTNQLSE-IVTPVLAVAGSVD
Mb3196c|M.bovis_AF2122/97           LVYELFTQMSAAGRGG--CAKMLVEEVGSAHLNLDG-LTVPTLVIGGVRD
Rv3171c|M.tuberculosis_H37Rv        LVYELFTQTSAAGRGG--CAKMLVEEVGSAHLNLDG-LTVPTLVIGGVRD
MMAR_1388|M.marinum_M               LLYELFAQTSAAGRGG--CARMLVGEVGSRHISLDG-LTVPTLVIGSERD
MUL_2488|M.ulcerans_Agy99           LLYELFAQTSAAGRGG--CARMLVGEVGSRHISLDG-LTVPTLVIGSERD
MAB_0349c|M.abscessus_ATCC_199      SIRAELRDLRVDSVYW--AVNSVVPARPDQRELLGR-ITTPILVIAGRED
MAV_4589|M.avium_104                DWIAMFSTWPVKSTPG--MRAQLDVAPYTNRLSAYRSIATPVLVIGFSDD
TH_2963|M.thermoresistible__bu      EWERRLRRYPRSSVRD--AVLAVANRAG-VEDELAA-IDVPTLVIGGAQD
MLBr_01899|M.leprae_Br4923          VRTNAAEIYDRSFYPLGVSRQFSAILGSGSLLHYNQRIIAPTVVIHGRAD
                                                                         : .* :.:    *

MSMEG_1897|M.smegmatis_MC2_155      PATPPDRLAEITAAVRGARLVQVTPGAHLANVEQADAITPTLLEHLGTEQ
Mflv_1348|M.gilvum_PYR-GCK          PATPPAKLEQIVSAIPGARLLVVDGAAHLANAEQPEQITPAIIEHL--EQ
Mvan_0563|M.vanbaalenii_PYR-1       VPTPPESLRRIASDVKDGDLVVLEGVGHLAPAEAPQRVAGLIAEIVGVPQ
Mb3196c|M.bovis_AF2122/97           RLTPISQSRRIARTAPNVVGLVELPGGHCSMLERHQEVNSHLRALAESVT
Rv3171c|M.tuberculosis_H37Rv        RLTPISQSRRIARTAPNVVGLVELPGGHCSMLERHQEVNSHLRALAESVT
MMAR_1388|M.marinum_M               RLTPISQTRKIASTAPNVFDLVELPGGHCSMLEQHHEVNRHLRTLVDSVG
MUL_2488|M.ulcerans_Agy99           RLTPISQTRKIASTAPNVFDLVELPGGHCSMLEQHHEVNRHLRTLVDSVG
MAB_0349c|M.abscessus_ATCC_199      PTFPVAETELMAEAIPGAELVIMENTGHLAALERPEEVNALIDDFLKRHP
MAV_4589|M.avium_104                VLTPPYLGREVADALPNGRYVQIPDTGHLGFFERPDAVNAAMLKFFGEQH
TH_2963|M.thermoresistible__bu      AATPPAHAQALAAGIRGARLEIIDDCGHSSPLERPDVVTELLRGFLLEHT
MLBr_01899|M.leprae_Br4923          KLVRPSSGRAVARAITGARLVLFDGMGHDLPQQLWDQAVGVLMSNFAKAS
                                              :.    .         .*    :  .     :        

MSMEG_1897|M.smegmatis_MC2_155      S-------------------------------------------------
Mflv_1348|M.gilvum_PYR-GCK          R-------------------------------------------------
Mvan_0563|M.vanbaalenii_PYR-1       PPSKTLEDVHRAGMAVRREVLGEAHVDRAVAGTTDLTADFQHMITQYAWG
Mb3196c|M.bovis_AF2122/97           RHVRDRRISS----------------------------------------
Rv3171c|M.tuberculosis_H37Rv        RHVRDRRISS----------------------------------------
MMAR_1388|M.marinum_M               GRSRAR-ISS----------------------------------------
MUL_2488|M.ulcerans_Agy99           GRSRAR-ISS----------------------------------------
MAB_0349c|M.abscessus_ATCC_199      IED-----------------------------------------------
MAV_4589|M.avium_104                --------------------------------------------------
TH_2963|M.thermoresistible__bu      --------------------------------------------------
MLBr_01899|M.leprae_Br4923          --------------------------------------------------
                                                                                      

MSMEG_1897|M.smegmatis_MC2_155      --------------------------------------------------
Mflv_1348|M.gilvum_PYR-GCK          --------------------------------------------------
Mvan_0563|M.vanbaalenii_PYR-1       TIWTRPGLDLRSRSMITLTALVARGHHEELAMHLRAARRNGLSNDEIKEL
Mb3196c|M.bovis_AF2122/97           --------------------------------------------------
Rv3171c|M.tuberculosis_H37Rv        --------------------------------------------------
MMAR_1388|M.marinum_M               --------------------------------------------------
MUL_2488|M.ulcerans_Agy99           --------------------------------------------------
MAB_0349c|M.abscessus_ATCC_199      --------------------------------------------------
MAV_4589|M.avium_104                --------------------------------------------------
TH_2963|M.thermoresistible__bu      --------------------------------------------------
MLBr_01899|M.leprae_Br4923          --------------------------------------------------
                                                                                      

MSMEG_1897|M.smegmatis_MC2_155      -----------------------------------
Mflv_1348|M.gilvum_PYR-GCK          -----------------------------------
Mvan_0563|M.vanbaalenii_PYR-1       LMQTAIYCGVPDANSAFRIAAEVLPEFDEHPGAPS
Mb3196c|M.bovis_AF2122/97           -----------------------------------
Rv3171c|M.tuberculosis_H37Rv        -----------------------------------
MMAR_1388|M.marinum_M               -----------------------------------
MUL_2488|M.ulcerans_Agy99           -----------------------------------
MAB_0349c|M.abscessus_ATCC_199      -----------------------------------
MAV_4589|M.avium_104                -----------------------------------
TH_2963|M.thermoresistible__bu      -----------------------------------
MLBr_01899|M.leprae_Br4923          -----------------------------------