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NPNLPLVNAANSAGDNLNISPTTLPGRSHLTSMRPRQTRDRYRVVPEGYHPIPGRNRHPGTMVGNTPVLW VSGDASPVGSADRGFWAKLEGFNPGGMKDRPAMHMVERARARGELAPGAAIVESTSGTLGLGLALAGSVY GHPVTLVTDPGLEPIIARMLTAYGARVDMVTEPHPVGGWQQARKDRVAQLLAADPSAWNPDQYSNPDNVD AYRPLALEMAAQLGRVDVLVCSVGTGGHSSGVARVLREFNPAMRLIGVDTIGSTIFGQPASSRLMRGLGS SIFPRNVDYSAFNEVHWVAPAEAVWACRSMAATHYASGGWSVGAVALVAGWAARTLPSDITIAAVFPDGP QRYFDTVYNDDYCNEHGLLGQRPATEPEVIASPSAAVVQRWTRSATVIDPTQVAA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_4768 | cysK2 | - | 100% (406) | cysteine synthase a CysK2 |
| M. marinum M | MMAR_4390 | cbs | 4e-32 | 31.15% (321) | cystathionine beta-synthase CBS |
| M. marinum M | MMAR_3645 | cysK1 | 7e-26 | 32.38% (281) | cysteine synthase a CysK1 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0871 | cysK2 | 0.0 | 83.11% (373) | cysteine synthase A CysK2 |
| M. gilvum PYR-GCK | Mflv_0899 | - | 1e-156 | 68.88% (376) | pyridoxal-5'-phosphate-dependent enzyme, beta subunit |
| M. tuberculosis H37Rv | Rv0848 | cysK2 | 0.0 | 83.11% (373) | cysteine synthase A CysK2 |
| M. leprae Br4923 | MLBr_02396 | cysM2 | 2e-32 | 30.94% (320) | cystathionine beta-synthase |
| M. abscessus ATCC 19977 | MAB_1273 | - | 1e-150 | 73.98% (342) | cysteine synthase A CysK2 |
| M. avium 104 | MAV_1201 | - | 1e-32 | 30.86% (324) | cystathionine beta-synthase |
| M. smegmatis MC2 155 | MSMEG_5270 | - | 2e-35 | 32.41% (324) | cystathionine beta-synthase |
| M. thermoresistible (build 8) | TH_1371 | - | 3e-33 | 31.69% (325) | cystathionine beta-synthase |
| M. ulcerans Agy99 | MUL_0203 | cbs | 3e-32 | 30.84% (321) | cystathionine beta-synthase CBS |
| M. vanbaalenii PYR-1 | Mvan_5856 | - | 1e-155 | 73.71% (350) | pyridoxal-5'-phosphate-dependent enzyme, beta subunit |
CLUSTAL 2.0.9 multiple sequence alignment
MAV_1201|M.avium_104 --------------------------------------------------
MUL_0203|M.ulcerans_Agy99 --------------------------------------------------
MLBr_02396|M.leprae_Br4923 --------------------------------------------------
MSMEG_5270|M.smegmatis_MC2_155 --------------------------------------------------
TH_1371|M.thermoresistible__bu --------------------------------------------------
Mb0871|M.bovis_AF2122/97 ---------------------------------MRSRQT-----------
Rv0848|M.tuberculosis_H37Rv ---------------------------------MRSRQT-----------
MMAR_4768|M.marinum_M MNPNLPLVNAANSAGDNLNISPTTLPGRSHLTSMRPRQT-----------
Mflv_0899|M.gilvum_PYR-GCK ---------------------------------MNHALS-----------
Mvan_5856|M.vanbaalenii_PYR-1 ---------------------------------MNHVLS-----------
MAB_1273|M.abscessus_ATCC_1997 MRPARAATSSPISALFGADRPSGTTGFSRHAHTRRHQLPGRFAAALTQGF
MAV_1201|M.avium_104 -----------------MRIAQHISDLIGGTPLVRLN---SVVPDGAGIV
MUL_0203|M.ulcerans_Agy99 -----------------MRIAQHISDLIGGTPLVRLN---AVVPAGIGTV
MLBr_02396|M.leprae_Br4923 -----------------MRIAQHISDLIGGTPLVRLN---SVVPAGTGVV
MSMEG_5270|M.smegmatis_MC2_155 -----------------MRIARHISELIGNTPLVQLN---SVVPEGAGLV
TH_1371|M.thermoresistible__bu -----------------MRYARHVSELIGNTPLVQLN---SVVPEGAGMV
Mb0871|M.bovis_AF2122/97 --RDRYRLLPEGYQVTPG-RNRHPGTMVGNTPVLWIP-ELSGTSDPDRGF
Rv0848|M.tuberculosis_H37Rv --RDRYRLLPEGYQVTPG-RNRHPGTMVGNTPVLWIP-ELSGTSDPDRGF
MMAR_4768|M.marinum_M --RDRYRVVPEGYHPIPG-RNRHPGTMVGNTPVLWVSGDASPVGSADRGF
Mflv_0899|M.gilvum_PYR-GCK --IHHAQDTDRFEQLRLG-RYERPATMVGQTPVLRIG---APFTAAEHGF
Mvan_5856|M.vanbaalenii_PYR-1 --IHHAQLS---EQRQLG-RYERPATMVGQTPVLRIT---VPFTDADRGF
MAB_1273|M.abscessus_ATCC_1997 SMNNSIALSSRRSCTHQGPKFHDPNTLVGNTPVLWVS---EPFSTPGTGF
. ::* **:: : .
MAV_1201|M.avium_104 AAKVEYLNPGGSSKDRIAVRMIDAAEASGALKPGGTIVEPTSGNTGVGLA
MUL_0203|M.ulcerans_Agy99 AAKVEYLNPGGSSKDRIAIKMIDAAEASGALRPGGTIVEPTSGNTGVGLA
MLBr_02396|M.leprae_Br4923 AAKVEYFNSGGSSKDRIAAKMIDAAEASGQLKPGGTIVEPTSGNTGVGLA
MSMEG_5270|M.smegmatis_MC2_155 AAKIEYLNPGGSSKDRIALKMIEAAEVSGELKPGGTIVEPTSGNTGVGLA
TH_1371|M.thermoresistible__bu LAKIEYLNPGGSSKDRIAVKMIEDAEKRGLLKPGGTIVEPTSGNTGVGLA
Mb0871|M.bovis_AF2122/97 WAKLEGFNPGG-MKDRPALYMVECARARGDIAPGAAIVESTSGTLGLGLA
Rv0848|M.tuberculosis_H37Rv WAKLEGFNPGG-MKDRPALYMVECARARGDIAPGAAIVESTGGTLGLGLA
MMAR_4768|M.marinum_M WAKLEGFNPGG-MKDRPAMHMVERARARGELAPGAAIVESTSGTLGLGLA
Mflv_0899|M.gilvum_PYR-GCK WAKLEGFNPGG-MKDRPALHMVERARSRGKLVPGARIVESTSGTLGLGLA
Mvan_5856|M.vanbaalenii_PYR-1 WAKLEGFNPGG-MKDRPALHMVQRARAAGALKPGARIVESTSGTLGLGLA
MAB_1273|M.abscessus_ATCC_1997 WAKLEGHNPNG-MKDRPALHMIEQARARGDLKPGAMIVESTSGTLGLGLA
**:* *..* *** * *:: *. * : **. ***.*.*. *:***
MAV_1201|M.avium_104 LVAQQRGYKCVFVCPDKVGEDKRNVLMAYGAEVVVCPTAVP-PEHPDSYY
MUL_0203|M.ulcerans_Agy99 LVAQHRGYKCVFVCPDKVSEDKRNVLRAYGAEVVVCPTAVP-PEDPDSYY
MLBr_02396|M.leprae_Br4923 LVAQHRGYKCVFVCPDKVSEDKRNVLLAYGAEVVVCPTAVP-PDDPDSYY
MSMEG_5270|M.smegmatis_MC2_155 LVAQQRGYKCVFVCPDKVSEDKRNVLRAYGAEVVVCPTAVP-PDHPDSYY
TH_1371|M.thermoresistible__bu IVAQQRGYKCIFVCPDKVSEDKQNVLRAYGAEVVVCPTAVP-PEHPDSYY
Mb0871|M.bovis_AF2122/97 LAGKVYRHPVTLVTDPGLEPIIARMLTAYGAGVDMGTQPHPVGGWQQARK
Rv0848|M.tuberculosis_H37Rv LAGKVYRHPVTLVTDPGLEPIIARMLTAYGAGVDMVTQPHPVGGWQQARK
MMAR_4768|M.marinum_M LAGSVYGHPVTLVTDPGLEPIIARMLTAYGARVDMVTEPHPVGGWQQARK
Mflv_0899|M.gilvum_PYR-GCK LAGTVYGHPVTLVTDPGMEPMIQHMLAAFGAHIELVTEPHPRGGWQQART
Mvan_5856|M.vanbaalenii_PYR-1 LAGTGYGHPVTLVTDPGLEPIVARMLTAFGAHIELVVEPHQHGGWQQARR
MAB_1273|M.abscessus_ATCC_1997 LAGIIYRHPVTLVTDPGLEPIVSSMLNAYGAQVDIVTEPHPIGGWQQARK
:.. : :* : :* *:** : : . ::
MAV_1201|M.avium_104 SVSDRLVTEIEGAWKPDQYANPEGPASHYATTGPEIWADTDGKVTHFVAG
MUL_0203|M.ulcerans_Agy99 SVSDRLVREIDGAWKPDQYANPEGPASHYATTGPEIWADTDGKITHFVAG
MLBr_02396|M.leprae_Br4923 SVSDRLVAKIDGAWKPDQYANPAGPASHYETTGPEIWADTDGRVTHFVAG
MSMEG_5270|M.smegmatis_MC2_155 SVSNRLVTEIEGAWKPDQYSNQMGPESHYETTGPEIWADTEGKITHFVAG
TH_1371|M.thermoresistible__bu SVSDRLTREIDGAWKPDQYSNQMGPQSHYETTGPEIWNDTDGKVTHFVAG
Mb0871|M.bovis_AF2122/97 DRVAQLMAEYPGAWNPNQYGNPDN-VGAYRSLALELVAQLG-RIDVLVCS
Rv0848|M.tuberculosis_H37Rv DRVAQLMAEYPGAWNPNQYGNPDN-VGAYRSLALELVAQLG-RIDVLVCS
MMAR_4768|M.marinum_M DRVAQLLAADPSAWNPDQYSNPDN-VDAYRPLALEMAAQLG-RVDVLVCS
Mflv_0899|M.gilvum_PYR-GCK ERVHEILGTDPQAWHPDQYNNPDN-VEAYRGLAMELIEQLG-SVDVLVCS
Mvan_5856|M.vanbaalenii_PYR-1 ERVQQILSSDPHAWHPDQYNNPDN-VEAYRGLAHELIDQLG-SVDVLVCS
MAB_1273|M.abscessus_ATCC_1997 DRVAQLLAAEPDSWCPDQYQNPDN-VDAYRGLAEELIDQLG-TVDTLVCS
. .: :* *:** * . * . *: : : :*..
MAV_1201|M.avium_104 IGTGGTITGAGRYLKEVSG----GRVRVIGADPEGSVYSGGTGRPYLVEG
MUL_0203|M.ulcerans_Agy99 IGTGGTITGAGCYLKEVSG----GKVRVIGADPEGSVYSGGTGRPYLVEG
MLBr_02396|M.leprae_Br4923 IGTGGTITGVGRYLKEVSG----GLVRIIGADPEGSVYSGGTGRPYLVEG
MSMEG_5270|M.smegmatis_MC2_155 VGTGGTITGTGRYLKEVSG----GKVKVIGADPEGSVYSGGTGRPYLVEG
TH_1371|M.thermoresistible__bu VGTGGTITGAGRYLKEVSGRRPEGPVRVVGADPEGSVYSGGTGRPYLVEG
Mb0871|M.bovis_AF2122/97 VGTGGHSAGVARVLREFNP-----DMRLIGVDTIGSTIFGQPASNRLMRG
Rv0848|M.tuberculosis_H37Rv VGTGGHSAGVARVLREFNP-----DMRLIGVDTIGSTIFGQPASNRLMRG
MMAR_4768|M.marinum_M VGTGGHSSGVARVLREFNP-----AMRLIGVDTIGSTIFGQPASSRLMRG
Mflv_0899|M.gilvum_PYR-GCK VGTGGHSAGVARVLREYNP-----GLRLIGVDTVGSTIFGQPATTRLMRG
Mvan_5856|M.vanbaalenii_PYR-1 VGTGGHSAGVARVLRETDP-----QLRLIGVDTVGSTIFGQPAGARLMRG
MAB_1273|M.abscessus_ATCC_1997 VGTGGHSSGVGRVLREHNP-----NLRLVGVDTIGSTIFGQPAGSRLMRG
:**** :*.. *:* . ::::*.*. **. * .. *:.*
MAV_1201|M.avium_104 VGEDFWPQAYDRTVPDEIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVV
MUL_0203|M.ulcerans_Agy99 VGEDFWPATYDHAVPDEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVV
MLBr_02396|M.leprae_Br4923 VGEDFWPAAYDPMVPDQIIAVSDSDSFNMTRRLAREEAMLVGGSCGMAVV
MSMEG_5270|M.smegmatis_MC2_155 VGEDFWPTAYDPSVPDEIIAVSDADSFEMTRRLAREEALLVGGSCGMAVV
TH_1371|M.thermoresistible__bu VGEDFWPSVYDPSVPDEIIAVSDADSFEMTRRLAREEGLLVGGSCGMAVV
Mb0871|M.bovis_AF2122/97 LGSSIYPRNVDYRAFDEVHWVAPPEAVWACRSLAATHYASGGWSVGAVAL
Rv0848|M.tuberculosis_H37Rv LGSSIYPRNVDYRAFDEVHWVAPPEAVWACRSLAATHYASGGWSVGAVAL
MMAR_4768|M.marinum_M LGSSIFPRNVDYSAFNEVHWVAPAEAVWACRSMAATHYASGGWSVGAVAL
Mflv_0899|M.gilvum_PYR-GCK LGSSIYPGNVDYAAFNEVHWVAPADAVWACRTLAATHYASGGWSVGAVAV
Mvan_5856|M.vanbaalenii_PYR-1 LGSSIYPGNVDYAAFDEVHWVAPAEAVWACRTLAATHYASGGWSVGAVAL
MAB_1273|M.abscessus_ATCC_1997 LGSSIYPGNVDYPAFDEAHWVAPNEAVWAARTLAATYHASGGWSVGAVAL
:*..::* * . :: *: ::. * :* * * * ..:
MAV_1201|M.avium_104 AALRVAEQAGPDALVVVLLPDGGRGYMAKIFNDAWMSSYGFLRTRLDGST
MUL_0203|M.ulcerans_Agy99 AALRVAEQAGPDALVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSRLDGRT
MLBr_02396|M.leprae_Br4923 AALQVAEEAGPAALVVVLLPDGGRGYMSKIFNDAWMSSYGFLRSRLDGST
MSMEG_5270|M.smegmatis_MC2_155 AAIEVARKAGPDAVVVVLLPDGGRGYLSKIFNDAWMSSYGFLRTRLDGSV
TH_1371|M.thermoresistible__bu AANKVALEAGPDAVVVVLLPDGGRGYLSKIFNDEWMSSYGFLRTPLDGKV
Mb0871|M.bovis_AF2122/97 VAGWAARNLPADTTIAAVFPDGPQRYFDTIYNDAYCNEHELLGGQPP---
Rv0848|M.tuberculosis_H37Rv VAGWAARNLPADTTIAAVFPDGPQRYFDTIYNDAYCNEHELLGGQPP---
MMAR_4768|M.marinum_M VAGWAARTLPSDITIAAVFPDGPQRYFDTVYNDDYCNEHGLLGQRPA---
Mflv_0899|M.gilvum_PYR-GCK VAGWAARGSDPGTTVAAIFPDGPQRYFDTIYNDEYCRTHGLLDKAPP---
Mvan_5856|M.vanbaalenii_PYR-1 VAGWAARNSPPATTVAAIFPDGPQRYFDTVYNDDYCRAHHLLGGAPP---
MAB_1273|M.abscessus_ATCC_1997 VAGWVARTSDPGARIAAIFPDGPMRYHGTIYDDDYCRSHDLLDRPPA---
.* .* . :..::*** * .:::* : : :*
MAV_1201|M.avium_104 DQSRVGDVLRRKSGELPDLVHTHPSETVRDAIGILREYGVSQMPVVGAEP
MUL_0203|M.ulcerans_Agy99 DTSTVGDVLRRKSGALPALVHTHPSETVRDAIGILREYGVSQMPVVGAEP
MLBr_02396|M.leprae_Br4923 EQPIVGDVLRRKSGALPDLVHTHPSETVRDAIGILREYAVSQIPVVGAEP
MSMEG_5270|M.smegmatis_MC2_155 EK-TVGDVLRGKSGALPDLVHTHPSETVRDAIGILREYGVSQMPVVGAEP
TH_1371|M.thermoresistible__bu HQPTVGDVLRGKSGELPDLVHTHPSETIRDAINILREYGVSQMPVVGAEP
Mb0871|M.bovis_AF2122/97 -----------------------------------------------TEP
Rv0848|M.tuberculosis_H37Rv -----------------------------------------------TEP
MMAR_4768|M.marinum_M -----------------------------------------------TEP
Mflv_0899|M.gilvum_PYR-GCK -----------------------------------------------NEP
Mvan_5856|M.vanbaalenii_PYR-1 -----------------------------------------------SHP
MAB_1273|M.abscessus_ATCC_1997 -----------------------------------------------QDP
.*
MAV_1201|M.avium_104 PVMAGEVAGSVSERELLSAVFEGRAKLADAVSLHMSPPLPMIGAGESVSA
MUL_0203|M.ulcerans_Agy99 PVMAGEVAGSVSERELLSAVFSGKAKLADAVSQHMSPPLKLIGAGELVGV
MLBr_02396|M.leprae_Br4923 PVMAGEVVGSVSERELLSAVFEGRASLADAVSEHMGPPLRLIGDGELVSV
MSMEG_5270|M.smegmatis_MC2_155 PVMAGEVAGSVSERELLSAVFEGRAKLADAVAEHMSPPLPLIGAGELVST
TH_1371|M.thermoresistible__bu PVMAGEVAGSVTERELLSAVYEGRAKLTDAVAEHMGAPLPLIGAGEPVQA
Mb0871|M.bovis_AF2122/97 DEIASPLDAVVTRWTRSTTVIDPTQVVS----------------------
Rv0848|M.tuberculosis_H37Rv DEIASPLDAVVTRWTRSTTVIDPTQVVS----------------------
MMAR_4768|M.marinum_M EVIASPSAAVVQRWTRSATVIDPTQVAA----------------------
Mflv_0899|M.gilvum_PYR-GCK ATIDHPLRHAVKAWTRCPVVVDPTQVVPR---------------------
Mvan_5856|M.vanbaalenii_PYR-1 ATIADPLAQVATAWSRCTRIVDPTRVMPR---------------------
MAB_1273|M.abscessus_ATCC_1997 DVIDDPSERVVDRWTRLTRVIDPIGRRA----------------------
: . . : . .
MAV_1201|M.avium_104 AGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEGPRGSGGRR
MUL_0203|M.ulcerans_Agy99 AGKALRDWDALMVVEEGKPVGVITRYDLLGFLSEG----ALRR
MLBr_02396|M.leprae_Br4923 AGEALRDSDALMVVEKGKLVGVITRYDLLGFLSEG----APLR
MSMEG_5270|M.smegmatis_MC2_155 AAKTLRESDAVMVVEDGKPVGVLTRHDLLGFLSEG----GYRH
TH_1371|M.thermoresistible__bu AAKMLRECDAVMVVEEGKPAGVLTRHDLLGFLSES----TAAH
Mb0871|M.bovis_AF2122/97 -------------------------------------------
Rv0848|M.tuberculosis_H37Rv -------------------------------------------
MMAR_4768|M.marinum_M -------------------------------------------
Mflv_0899|M.gilvum_PYR-GCK -------------------------------------------
Mvan_5856|M.vanbaalenii_PYR-1 -------------------------------------------
MAB_1273|M.abscessus_ATCC_1997 -------------------------------------------