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IDLLASPQAMTATATEVAEIGLAINQADLAVAGPTTGLAAAAADEVSEAIATLFDNYAQGYQAVSRQAAA FHDAFAQTLFAASDSYVRAEAAAVKALAGLTEPVANLFAPVLSGPESVAASATALLSGAPLKPVEAALIL SESGVPTPSASYISDVYTKYVEPNFPSATAPQGVSTPNGLYPFTGIKDLTLDISLARGATILNDAILQQL AALPAGSSVAVLGYSQSAVLSSLVMPKLLAEGVTSSQVNFVLLGNPMNPNGGVLARFPDLTLPSLGFTFY GATPDNAFPTVSYTLEYDGFADFPRYPINLLADINAVMGIPFVHADYQHLTQAQLDSAVQLPTEGPTQST YYMIPTEHLPLLAPLRFIPYVGDPLAELLEPNLRVLVNLGYGDPAYGYDTGPANVPTPFGLFPPVSPLTV AEHLAAGTQQGIVNSGAALQAQGPPSIPLSDIAHTLTANPPWAGTPALPPSTSPASSIEDFILAVQAANS SISRGFVKTLSTAYATLLPTTDIASELVFTLPSYNLNLFLDGVIQAINGQPIEGLINAIGNPIAASTGLI TLAGGFQLIVISYALDTILFGTPHPLP*
Operon Prediction Model: Genebank
Paralogs
species | id | gene | e-value | identity (len) | annotation |
M. marinum M | MMAR_4321 | - | - | 100% (588) | PE family protein |
M. marinum M | MMAR_0371 | - | e-138 | 47.97% (590) | PE family protein |
M. marinum M | MMAR_0370 | - | e-135 | 48.29% (584) | PE family protein |
Closest Orthologs (e-value cutoff: 1e-4)
species | id | gene | e-value | identity (len) | annotation |
M. bovis AF2122 / 97 | Mb0156c | PE1 | 1e-134 | 48.28% (580) | PE family protein |
M. gilvum PYR-GCK | Mflv_1918 | - | 1e-33 | 35.02% (317) | hypothetical protein Mflv_1918 |
M. tuberculosis H37Rv | Rv0151c | PE1 | 1e-134 | 48.28% (580) | PE family protein |
M. leprae Br4923 | MLBr_01232 | - | 3e-11 | 25.09% (267) | hypothetical protein MLBr_01232 |
M. abscessus ATCC 19977 | MAB_4141 | - | 1e-31 | 31.49% (362) | PE/PPE family protein |
M. avium 104 | MAV_1346 | - | 1e-13 | 32.84% (204) | PE family protein |
M. smegmatis MC2 155 | MSMEG_5455 | - | 2e-35 | 33.33% (300) | PE family protein |
M. thermoresistible (build 8) | TH_3724 | - | 1e-42 | 37.15% (288) | PUTATIVE putative PE-PPE, C-terminal domain protein |
M. ulcerans Agy99 | MUL_0967 | - | 0.0 | 98.90% (543) | PE family protein |
M. vanbaalenii PYR-1 | Mvan_4726 | - | 1e-39 | 31.22% (442) | hypothetical protein Mvan_4726 |
CLUSTAL 2.0.9 multiple sequence alignment Mflv_1918|M.gilvum_PYR-GCK -------------------------------------------------- MSMEG_5455|M.smegmatis_MC2_155 -------------------------------------------------- TH_3724|M.thermoresistible__bu -------------------------------------------------- MMAR_4321|M.marinum_M ------------------------------MID--------LLASPQAMT MUL_0967|M.ulcerans_Agy99 ----------------------------MAVID--------LLASPQAMT Mb0156c|M.bovis_AF2122/97 MAPFGFTPKARHNRGVALRSTYRLDRWVMGPVDKEGWGLSYVFAQPSVLA Rv0151c|M.tuberculosis_H37Rv MAPFGFTPKARHNRGVALRSTYRLDGWVMGPVDKEGWGLSYVFAQPSVLA Mvan_4726|M.vanbaalenii_PYR-1 -------------------------------------------------M MAV_1346|M.avium_104 -------------------------------------------------- MAB_4141|M.abscessus_ATCC_1997 -------------------------------------------------- MLBr_01232|M.leprae_Br4923 -------------------------------------------------- Mflv_1918|M.gilvum_PYR-GCK ------MGKGDFGLKASEFTGRSSTRFADSVRRRPGGATYGLYRHDGLGV MSMEG_5455|M.smegmatis_MC2_155 ------MGRLLHSLSTAFLVFVAVVVLCVSTS------------------ TH_3724|M.thermoresistible__bu ----------VVAVGALTWGGXGVASPAGQPE------------------ MMAR_4321|M.marinum_M ATATEVAEIGLAINQADLAVAGPTTGLAAAAADEVSEAIATLFDNYAQGY MUL_0967|M.ulcerans_Agy99 ATATEVAEIGLAINQADLAVAGPTTGLAAAAAAEVSEAIATLFDNYAQGY Mb0156c|M.bovis_AF2122/97 AAATDLAGIGSAINQATAAVAAPTTGLAAAAADEVSTALATLFGAYGQQF Rv0151c|M.tuberculosis_H37Rv AAATDLAGIGSAINQATAAVAAPTTGLAAAAADEVSTALATLFGAYGQQF Mvan_4726|M.vanbaalenii_PYR-1 RRRLRSAAWGLLSLVIAVLFAWGSVVTSTSAG------------------ MAV_1346|M.avium_104 ---------------MSFVTTRPEALLA---------------------- MAB_4141|M.abscessus_ATCC_1997 -----MAGQYSVPGKRHSLRRYVATITAVAAT------------------ MLBr_01232|M.leprae_Br4923 --MKRLLATTLAALTVGTVSGFGSTVASSEPGE----------------- . . Mflv_1918|M.gilvum_PYR-GCK GTWAWVFARSSRSFPRSRRCWRYPRGVPTPVAV----------------- MSMEG_5455|M.smegmatis_MC2_155 --------------------------MPTAAHL----------------- TH_3724|M.thermoresistible__bu ---------------------------RAAALR----------------- MMAR_4321|M.marinum_M QAVSRQAAAFHDAFAQTLFAASDSYVRAEAAAVKALAGLTEPVANLFAPV MUL_0967|M.ulcerans_Agy99 QAVSRQTAAFHDAFAQTLFAASDSYVRAEAAAVKALAGLTEPVANLFAPV Mb0156c|M.bovis_AF2122/97 QAISAQVAAFHNEFTQRLAAAANAFVNAEATNTSALV--QEATAGLFKP- Rv0151c|M.tuberculosis_H37Rv QAISAQVAAFHNEFTQRLAAAANAFVNAEATNTSALV--QEATAGLFKP- Mvan_4726|M.vanbaalenii_PYR-1 -------------------------------------------------- MAV_1346|M.avium_104 -------------------------------------------------- MAB_4141|M.abscessus_ATCC_1997 -------------------------------------------------- MLBr_01232|M.leprae_Br4923 -----------------------PWLPPSPVPVR---------------- Mflv_1918|M.gilvum_PYR-GCK -------------------LVANTTALVMGGTFHPLTGPRDSPEFVTAYL MSMEG_5455|M.smegmatis_MC2_155 -------------------LLNHPHALVIGGTGTPVP----PPEYVNGQT TH_3724|M.thermoresistible__bu -------------------LTATADAIIMPGTKIGYIP---SQDEFTAYA MMAR_4321|M.marinum_M LSGPESVAASATALLSGAPLKPVEAALILSESGVPTP----SASYISDVY MUL_0967|M.ulcerans_Agy99 LSGPESVAASATALLSGAPLKPVEAALILSESGVPTP----SASYISDVY Mb0156c|M.bovis_AF2122/97 ---------TSPPVLP--PMFNQNTAIIMGGTGSPIP----TPSYVNAIT Rv0151c|M.tuberculosis_H37Rv ---------TSPPVLP--PMFNQNTAIIMGGTGSPIP----TPSYVNAIT Mvan_4726|M.vanbaalenii_PYR-1 ----------------------AGTALVMGGNGHPLSVPQDTPDYINKYV MAV_1346|M.avium_104 ------------------------VASMLEGLGSSMD----AQNAAAAAP MAB_4141|M.abscessus_ATCC_1997 ------------------CTVLAPLAMESRTIASTLASYVIGIGGNNDGA MLBr_01232|M.leprae_Br4923 ------------------ENSPAKIVYALGGARPPTFDWDYYTIRAGDEF . Mflv_1918|M.gilvum_PYR-GCK DNAVDNQISAAYGYAVTNAVAV-YTPEEFFPLGG---LTFDDSVAEGLAN MSMEG_5455|M.smegmatis_MC2_155 DNYIGAEFPGEYRYAVH-------TPQNFWPVYGS--MSFDESTVAGLAA TH_3724|M.thermoresistible__bu TAVIGATAGAPGAVDYLG------VPGEFWPATGLDSLTFDASVAXGQAM MMAR_4321|M.marinum_M TKYVEPNFPSATAPQGVS------TPNGLYPFTGIKDLTLDISLARGATI MUL_0967|M.ulcerans_Agy99 TKYVEPNFPSATAPQGVS------TPNGLYPFTGIKDLSLDISLARGATI Mb0156c|M.bovis_AF2122/97 TLFIDP-VVSNPVVKALV------TPEELYPITGVKSLPFQTSVQLGLQI Rv0151c|M.tuberculosis_H37Rv TLFIDP-VVSNPVVKALV------TPEELYPITGVKSLPFQTSVQLGLQI Mvan_4726|M.vanbaalenii_PYR-1 TAADDAFIAPSGLCGPQCSLVATYTPAQIRFVNGIFDMTYDESVAVGQAN MAV_1346|M.avium_104 TTSIAPAAADEVSALQAA----------QFSAYG----TWYQQVSAQAKA MAB_4141|M.abscessus_ATCC_1997 SANIPRKLGGAYSVDYHPVS----YPGAIWPVSGLTAPTFKTSVAQGRTN MLBr_01232|M.leprae_Br4923 FPDVNRKLIDYPARAPFR------YVPTFLVPGPRDEVTIGEAIAVATKN . Mflv_1918|M.gilvum_PYR-GCK LGRCLRAEPGCVFNADPAVAARVGTVAPRHDDTFVVFGYSQSAAVASLLK MSMEG_5455|M.smegmatis_MC2_155 LQAEMAKEG-------------------HFGSPLVILGYSSSTRILTADK TH_3724|M.thermoresistible__bu LTPMVDGAIES-------------------DTPIVVFGHSQSAVIATKEK MMAR_4321|M.marinum_M LNDAILQQLAAL----------------PAGSSVAVLGYSQSAVLSSLVM MUL_0967|M.ulcerans_Agy99 LNDAILQQLAAL----------------PAGSSVAVLGYSQSAVLSSRVM Mb0156c|M.bovis_AF2122/97 LDGAIWEQIN-------------------AGNHVTVFGYSQSAVIASLEM Rv0151c|M.tuberculosis_H37Rv LDGAIWEQIN-------------------AGNHVTVFGYSQSAVIASLEM Mvan_4726|M.vanbaalenii_PYR-1 LNDCIQGSDCTATLAPYSETG----PHTVADSTYVIYAYSQSATVAANQK MAV_1346|M.avium_104 IHQTLVQNLD---------------------TNADSYGATEAANRVN--- MAB_4141|M.abscessus_ATCC_1997 LNDEIVTAGN---------------------QPITIVGYSLGAVVVSKSH MLBr_01232|M.leprae_Br4923 LNQAIHRGT----------------------EPAAVVGLSQGSLALDTEQ : : . : .: Mflv_1918|M.gilvum_PYR-GCK RQLIDAP--GPEPAATTFMLAANPMRPNGG----VLGRF-PALTIPILGI MSMEG_5455|M.smegmatis_MC2_155 NRRIAD---GDYAVDINYVLVSDVAKGNGG----VMARF-PGWHIPILGV TH_3724|M.thermoresistible__bu QRLLAEYPDPDTAPDVTFVMVANPNRPNGG----LLARF-PGLYIPILGV MMAR_4321|M.marinum_M PKLLAEG---VTSSQVNFVLLGNPMNPNGG----VLARF-PDLTLPSLGF MUL_0967|M.ulcerans_Agy99 PKLLAEG---VTSSQVNFVLLGNPMNPNGG----VLARF-PDLTLPSLGF Mb0156c|M.bovis_AF2122/97 QHLISLGPNAPSPSQLNFILIGNEMNPNGG----ILARI-PGLNVTTLGL Rv0151c|M.tuberculosis_H37Rv QHLISLGPNAPSPSQLNFILIGNEMNPNGG----ILARI-PGLNVTTLGL Mvan_4726|M.vanbaalenii_PYR-1 LQLIAHP----VASTVSFLLVANPNRPDGG----ILERF-VGAYIPLLGI MAV_1346|M.avium_104 ------------TSAAALQGLAPNVIPNAA----PAADP-PPAGTTSLGT MAB_4141|M.abscessus_ATCC_1997 SDLLQRYP--GGNPNIQFVLMGSANTPNGG----IFARF-PWLKIPLADI MLBr_01232|M.leprae_Br4923 EQLATDP-TAPPPDQLTFNTFGDPSGYHGFGKSVLASIFRPGDYIPLIDY . .. . . Mflv_1918|M.gilvum_PYR-GCK TFHGATPTAG-VDGAYRTVDVVRQYDGLGGDFPVRPLNLLATVNALLGYG MSMEG_5455|M.smegmatis_MC2_155 TFDGATRTDS-DGYAFDTKSISFVHDGWS-DFPVYPLNVLALANAVAGIA TH_3724|M.thermoresistible__bu TFNGATPAPA-DG-PFDSYDVAREFDGWA-DFPRYPLNLVATANAILGIY MMAR_4321|M.marinum_M TFYGATPD-----NAFPTVSYTLEYDGFA-DFPRYPINLLADINAVMGIP MUL_0967|M.ulcerans_Agy99 TFYGATPD-----NAFPTVSYTLEYDGFA-DFPRYPINLLADINAVMGIP Mb0156c|M.bovis_AF2122/97 PFYGATPD-----NPYPTTTYTLEYDGFA-DFPRYPLNVLSDINAVFGIL Rv0151c|M.tuberculosis_H37Rv PFYGATPD-----NPYPTTTYTLEYDGFA-DFPRYPLNVLSDINAVFGIL Mvan_4726|M.vanbaalenii_PYR-1 TFNGSTPTFSPQPRPLTTVDVARQYDGWA-DFPTNPLNLLADLNAIMGTI MAV_1346|M.avium_104 SIG-----------------WAQNVGAAASDFITLGEGQFAPVPGAAGIP MAB_4141|M.abscessus_ATCC_1997 ESNGAWEP-----DQFKTKVINTEYDTYG-DFPAY-FNPLALANSIAAIQ MLBr_01232|M.leprae_Br4923 TMPQRMDS------QYDSNRVVAAYDGLS-EFPDRADNLLAQLNCFAGGA . . :* . .: . Mflv_1918|M.gilvum_PYR-GCK IMHG--ETVDVPF--------AQARFQGREGDTDYYLIGTDLLPLLQPLL MSMEG_5455|M.smegmatis_MC2_155 VLHP--TYPELDR--------PDLVPVGSTGDTEYFVIGTDIMPLLMPLE TH_3724|M.thermoresistible__bu YLHG--GYYELLTENPDILDDPERTIVQQYGDTTYYLIRTEQLPLLRPLR MMAR_4321|M.marinum_M FVHA--DYQHLTQAQLDS---AVQLPTEGPTQSTYYMIPTEHLPLLAPLR MUL_0967|M.ulcerans_Agy99 FVHA--DYQDLTQAQLDS---AVQLPTEGPTQSTYYMIPTEHLPLLAPLR Mb0156c|M.bovis_AF2122/97 TVHT--TYADLTPAQIAS---ATQLPTQGTTSNTYYIIETEHLPLLAPLR Rv0151c|M.tuberculosis_H37Rv TVHT--TYADLTPAQIAS---ATQLPTQGTTSNTYYIIETEHLPLLAPLR Mvan_4726|M.vanbaalenii_PYR-1 LLHP--NYSDAGT----------PQLQGQYQDSTYYLIPAPITPVLVPLT MAV_1346|M.avium_104 NFNP--GLTAATTAG----------------------------------- MAB_4141|M.abscessus_ATCC_1997 YAHPD-AYYDRIDPAALDDPDNPNVVKTVQGNTTYYLVRAEHLPLLQPLR MLBr_01232|M.leprae_Br4923 ISHTPSGFFNPEDVPPQN---IRTTVNSRGAKTTTYLIPVNHLPLTLPLR : Mflv_1918|M.gilvum_PYR-GCK LF---VPRPILSALDAP-LRVLVEDAYDR------DAGPGDPTTARWWP- MSMEG_5455|M.smegmatis_MC2_155 TIG--VPRPILLAVDEP-LRVLVEAGYRR------DIAPGAPTPARLIP- TH_3724|M.thermoresistible__bu DLG--VPEEFVDALEPY-VRRIVDLGYDR------NTPYGEPAPVRVLP- MMAR_4321|M.marinum_M FIP--YVGDPLAELLEPNLRVLVNLGYGDPAYGYDTGPANVPTPFGLFP- MUL_0967|M.ulcerans_Agy99 FIP--YVGDPLAELLEPNLRVLVNFGYGDPAYGYDTGPANVPTPFGLFP- Mb0156c|M.bovis_AF2122/97 AIP--VIGPPLAALVEPNLEVIVNLGYGDPRFGYSTSPANVPTPFGLFP- Rv0151c|M.tuberculosis_H37Rv AIP--VIGPPLAALVEPNLEVIVNLGYGDPRFGYSTSPANVPTPFGLFP- Mvan_4726|M.vanbaalenii_PYR-1 WVP--IVGKPLAIALDPAVRVLVEMGYDR------TVNPGQPTPARYLY- MAV_1346|M.avium_104 ------PPAPAAPAAAAGAPVLASMGRGP-------SIGGLSVPPSWAP- MAB_4141|M.abscessus_ATCC_1997 DLTNPIGASFVLDAIEPTLRVFIDMAYDR------DTGPGTTTQFSFFTP MLBr_01232|M.leprae_Br4923 YLG---WSDALVDQIDAVLQPKIDAAYAYN-----DNPLNKPISVDPVNG . . . . Mflv_1918|M.gilvum_PYR-GCK VKDFAGTAAKLIASVPVAVDNLTEGFGLGRVL----------------GT MSMEG_5455|M.smegmatis_MC2_155 IVNPITLTRNFIAAIPVGVDDAMEELGHGRPL----------------GT TH_3724|M.thermoresistible__bu PLTPAPG--------PATQHNAGQGITVASAT----------------ST MMAR_4321|M.marinum_M PVSPLTVAEHLAAGTQQGIVNSGAALQAQGPP----------------SI MUL_0967|M.ulcerans_Agy99 PVSPLTVAEHLAAGTQQGIVNSGAALQAQGPP----------------SI Mb0156c|M.bovis_AF2122/97 DVPASVVADALVAGTQQGVNDFMVELPA---------------------- Rv0151c|M.tuberculosis_H37Rv DVPASVVADALVAGTQQGVNDFMVELPA---------------------- Mvan_4726|M.vanbaalenii_PYR-1 VPNLIKAALNFAMAIPTGWDDAISYLTGDPDCR------------PFHTT MAV_1346|M.avium_104 GGVAPAANATPAQLVGAGFTSAAPDTAP---------------------- MAB_4141|M.abscessus_ATCC_1997 SKNIVDAFNKLPTAIAEGAHNVENGLAGATPKQSASPEKPASPPVPQPGP MLBr_01232|M.leprae_Br4923 MDPIAGIDAELRDSILNVFAQLRSILPPPPG------------------- . Mflv_1918|M.gilvum_PYR-GCK PVPGVFGVGG-----------------PDLPDVDEGGAVTSDIDEDAVAQ MSMEG_5455|M.smegmatis_MC2_155 QPSGVFGVGGEDTELKGLPAGVISLAKPTLPVSSHTTVVEKTERKDLAKP TH_3724|M.thermoresistible__bu P-------------------------------DKRTPVVEAPVEE---PP MMAR_4321|M.marinum_M PLSDIAHT-LTANPP--------WAGTPALPPSTSPASSIEDFILAVQAA MUL_0967|M.ulcerans_Agy99 PLSDIAHT-LTANPP--------WAGTPALPPSTSPASSIEDFILAVQAA Mb0156c|M.bovis_AF2122/97 ALNTLPQTPMPAFPP--------YVPT-LLPPPPPPQP----------AT Rv0151c|M.tuberculosis_H37Rv ALNTLPQTPMPAFPP--------YVPT-LLPPPPPPQP----------AT Mvan_4726|M.vanbaalenii_PYR-1 VPASPFGVGGPPVYTGAIDPYGEPTPYLAPPVETHGEAAPGSGSPAAHPA MAV_1346|M.avium_104 -------------------------------------------------- MAB_4141|M.abscessus_ATCC_1997 TNPGSATTQIPAPEAPTPSVVSLSTAEPPAPPASAKAADPTAISVSTAGA MLBr_01232|M.leprae_Br4923 -------------------------------------------------- Mflv_1918|M.gilvum_PYR-GCK RDSASSTERSAPAEESR--SAVGGADSDGRDD------VVDPEPAAPEAE MSMEG_5455|M.smegmatis_MC2_155 AAEPEPVVEDEPAVEDE--TPVADTKKASPKP------LRPKVRGPISFD TH_3724|M.thermoresistible__bu VQTGRSTLKREKPVSAR--HLVRNSLRAVPGL------RRGEPAATPTPD MMAR_4321|M.marinum_M NSSISRGFVKTLSTAYA--TLLPTTDIASELV------FTLPSYNLNLFL MUL_0967|M.ulcerans_Agy99 NSSISRGFVKTLSTAYA--TLLPTTDIASELV------FTLPSYNLNLFL Mb0156c|M.bovis_AF2122/97 LINIADTFASVVSTGYS--ILLPTADLGLAFV------TILPAYDLTLFV Rv0151c|M.tuberculosis_H37Rv LINIADTFASVVSTGYS--ILLPTADLGLAFV------TILPAYDLTLFV Mvan_4726|M.vanbaalenii_PYR-1 GTEPPETVTFSPTTESTPSTLLPETPAESEDMPPDFVENDTPDSDEVSPT MAV_1346|M.avium_104 VTAVPGGVPSMATAGRG-------------------------GYGLGAPR MAB_4141|M.abscessus_ATCC_1997 STARDQDFQPDTKPDPKSTPPPTKDSVGEADKSKNTLKPTLRLPKLPVAQ MLBr_01232|M.leprae_Br4923 -------------------------------------------------- Mflv_1918|M.gilvum_PYR-GCK TSEAETVTETEADD---------ADDADDGADADDEDIDADGADADGEDT MSMEG_5455|M.smegmatis_MC2_155 APKLPTIRKATGDRPLKRLLSALTPKRPKPASSHDAGPAAGNKDTDNADK TH_3724|M.thermoresistible__bu VTPDVTPDAPAGQP--------------EPAEPNESAAPPAAAEADDASE MMAR_4321|M.marinum_M DGVIQAINGQPIEGLINAIGNPIAASTGLITLAGGFQLIVISYALDTILF MUL_0967|M.ulcerans_Agy99 DGVIQAINGQPIGD------------------------------------ Mb0156c|M.bovis_AF2122/97 NQLAAGNLRAAIEL-------PLAATIGLAALGGMIEFIAIVVTLADITQ Rv0151c|M.tuberculosis_H37Rv NQLAAGNLRAAIEL-------PLAATIGLAALGGMIEFIAIVVTLADITQ Mvan_4726|M.vanbaalenii_PYR-1 EDDALKDTETINESQDGPTESASGVSGEDAADADSRSADADSLSADAGDS MAV_1346|M.avium_104 YGVKPTVMPKPAI------------------------------------- MAB_4141|M.abscessus_ATCC_1997 PGTAPKSPKFPSLKNHLKSVPGLSEKKNPAADASSKHSQPANAPAHTSDK MLBr_01232|M.leprae_Br4923 -------------------------------------------------- Mflv_1918|M.gilvum_PYR-GCK AGNTAEAA----- MSMEG_5455|M.smegmatis_MC2_155 PAARADDAA---- TH_3724|M.thermoresistible__bu ------------- MMAR_4321|M.marinum_M GTPHPLP------ MUL_0967|M.ulcerans_Agy99 ------------- Mb0156c|M.bovis_AF2122/97 QLQSFSI------ Rv0151c|M.tuberculosis_H37Rv QLQSFSI------ Mvan_4726|M.vanbaalenii_PYR-1 APADAAAADTAA- MAV_1346|M.avium_104 ------------- MAB_4141|M.abscessus_ATCC_1997 TSDHASETGRGAA MLBr_01232|M.leprae_Br4923 -------------