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MSTLAEQTRWMDATDQAALVAAGEASPSELLEAAIERIERINPALNAVVIRWFDHARETASADLPGGPFR GVPFLIKDLYALYAGQPISNGNLAFKQAHVIADADTTLVGRYRAAGLVIAGRTNSPELGSLPTTEPVAWG PTRNPWDTSRTPGGSSGGSAAAVASGMVPFAHASDGGGSIRIPASCCGLVGLKPSQGRITLGPLREESGM SVEHCLSRTVRDTAALLDATRGPGVGDTVIAPPPIRPYTEELGADPGRLRIGILDHHPRGGPVDAEVTHA TQAVGRLLESLGHHVDVAWPAALEDASFDSTFGALWSTNMGLAQRRFEEQLGRPLGEDEFELVNRAQADF AKHFTSVDYALALSAIAAFRRAIQSWWHDGWDLLLTPTVASLPVKLGTIVSTPEHPMAAMQRAGEFVPFT PPFNTSGQPAISLPLEWTPEGLPLGVQLVAAYGREDVLLRIASQLEQAKPWAQRTPDI
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_3565 | amiC_1 | - | 100% (478) | amidase AmiC_1 |
| M. marinum M | MMAR_4954 | amiC_2 | 1e-84 | 40.41% (490) | amidase, AmiC_2 |
| M. marinum M | MMAR_4176 | amiB2 | 3e-65 | 37.16% (479) | amidase AmiB2 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1294 | amiB2 | 3e-65 | 37.24% (478) | amidase |
| M. gilvum PYR-GCK | Mflv_1016 | - | 2e-89 | 41.88% (499) | amidase |
| M. tuberculosis H37Rv | Rv1263 | amiB2 | 3e-65 | 37.24% (478) | amidase |
| M. leprae Br4923 | MLBr_01596 | - | 2e-65 | 37.18% (468) | amidase |
| M. abscessus ATCC 19977 | MAB_2181c | - | 3e-86 | 41.87% (492) | putative amidase |
| M. avium 104 | MAV_0676 | - | 3e-89 | 41.45% (497) | 6-aminohexanoate-cyclic-dimer hydrolase |
| M. smegmatis MC2 155 | MSMEG_6673 | - | 3e-91 | 41.16% (498) | 6-aminohexanoate-cyclic-dimer hydrolase |
| M. thermoresistible (build 8) | TH_2520 | - | 3e-62 | 36.23% (472) | PUTATIVE Amidase |
| M. ulcerans Agy99 | MUL_2798 | amiC_1 | 0.0 | 99.37% (478) | amidase AmiC_1 |
| M. vanbaalenii PYR-1 | Mvan_5822 | - | 1e-92 | 42.71% (501) | amidase |
CLUSTAL 2.0.9 multiple sequence alignment
MLBr_01596|M.leprae_Br4923 MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEAVNPALG
TH_2520|M.thermoresistible__bu MQRIHAFGDDALSDLDAVGLAAELRARRVSVPELIRAAIARTEAVNPALN
MSMEG_6673|M.smegmatis_MC2_155 ------MNFDEYRTFDATGLAELVADKQVTAAELLEVAQARAEAVNPRIN
Mvan_5822|M.vanbaalenii_PYR-1 -----MVDFQEYRAQDATGLARLVAEKQVTADELLALAQARAAQVNPRIN
Mflv_1016|M.gilvum_PYR-GCK ------MDFLEYRSHDATGLAKLVADGDVTADELLTLARERAAAVNPKIN
MAB_2181c|M.abscessus_ATCC_199 ------MDFSDYSQHDATGLAELVATKQVTPAELLAAARARAADVNNKIG
MAV_0676|M.avium_104 ------------MALDATALAGLVADKQVTPAELLALARQRADAVNPRLN
MMAR_3565|M.marinum_M ----MSTLAEQTRWMDATDQAALVAAGEASPSELLEAAIERIERINPALN
MUL_2798|M.ulcerans_Agy99 ----MSTLAEQTRWMDATDQAALVVAGEASPSELLEAAIERIERINPALN
Mb1294|M.bovis_AF2122/97 ------MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLR
Rv1263|M.tuberculosis_H37Rv ------MDPTDLAFAGAAAQARMLADGALTAPMLLEVYLQRIERLDSHLR
.*. * : : :: * :: :
MLBr_01596|M.leprae_Br4923 GLAYEAFQWARQTASKAGS-------GFFSGVPTFIKDN-IDVAGQPTMR
TH_2520|M.thermoresistible__bu GLAYEAFDRALARAEAGPV------PGFFGGIPTYVKDN-AAVAGMPTME
MSMEG_6673|M.smegmatis_MC2_155 AIVREVPAAPADAA-----------PGPFTGVPFLIKDIGQDYAGVPSSR
Mvan_5822|M.vanbaalenii_PYR-1 AIVRDVPAAPAGIA-----------DGPFAGVPFLIKDLAQDYAGLPSSR
Mflv_1016|M.gilvum_PYR-GCK AIVRDVPAPVGGNR-----------GGPFAGVPFLIKDLAQDYAGLPTAS
MAB_2181c|M.abscessus_ATCC_199 AIVRTLPEADQQVQGDL--------TGPFAGVPFLIKDLGQDYKGHPTSG
MAV_0676|M.avium_104 AIVCRLDDVADRQAADPNL------SGPFAGVPFLLKDLGQEYRGFPTSC
MMAR_3565|M.marinum_M AVVIRWFDHARETASADLP----GG--PFRGVPFLIKDLYALYAGQPISN
MUL_2798|M.ulcerans_Agy99 AVVIRWFDHARETASADLP----GG--PFRGVPFLIKDLYALYAGQPISN
Mb1294|M.bovis_AF2122/97 AYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAIKDDVDI-AGEVTTY
Rv1263|M.tuberculosis_H37Rv AYRVVQFDRARAEAEAAQQRLDAGERLPLLGVPIAIKDDVDI-AGEVTTY
. : *:* :** *
MLBr_01596|M.leprae_Br4923 GSDAWVPRN--AFDDGEFTRLYLATGPVSLGKTQLSEFGFSASAEHMRLG
TH_2520|M.thermoresistible__bu GTDAWDPRP--QPAHGDFARLYLGTGVIPLGKTQMSEFGFSASAEHPRLG
MSMEG_6673|M.smegmatis_MC2_155 GSRALRSIP--VTEHATVVQRWIEAGLVIFGKTNTPEFGAKGITEPEAWG
Mvan_5822|M.vanbaalenii_PYR-1 GSRALMSLP--AAEHSTIVQRWIDAGVVIFGKTNTPEFGAKGITEPVAWG
Mflv_1016|M.gilvum_PYR-GCK GSRSLMSRP--AGEHATVVQRWLDAGLVIFGKTNTPEFGAKGITEPQAYG
MAB_2181c|M.abscessus_ATCC_199 GCRALSTLP--APEHSTIVRRWLDAGLVIFGKTNTPEFGSKGITEPELFG
MAV_0676|M.avium_104 GSRSLANDV--ADRHALVTQRFLDAGLVIFGKTNTPEFGAKGVTEPDYWG
MMAR_3565|M.marinum_M GNLAFKQAHVIADADTTLVGRYRAAGLVIAGRTNSPELGSLPTTEPVAWG
MUL_2798|M.ulcerans_Agy99 GNLAFKQAHVIADADTTLVGRYRAAGLVIAGRTNSPELGSLPTTEPVAWG
Mb1294|M.bovis_AF2122/97 GSAGHGPA---ATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFG
Rv1263|M.tuberculosis_H37Rv GSAGHGPA---ATSDAEVVRRLRAAGAVIIGKTNVPELMIMPFTESLAFG
* . . .. :* : *:*: .*: :* *
MLBr_01596|M.leprae_Br4923 PVRNPWDTDYTAGASSSGSGAFVAAGVVPMAHANDGGGSIRIPASCNGLV
TH_2520|M.thermoresistible__bu PVRNPWHPEHTAGASSSGSGAFVAAGVVPIAHANDGGGSIRIPAACNGLV
MSMEG_6673|M.smegmatis_MC2_155 PTRNPWNLTHTPGGSSGGSAAAVAAGIVPCAGANDGGGSIRIPAACCGLV
Mvan_5822|M.vanbaalenii_PYR-1 PTRNPWDVTRTPGGSSGGSAAAVAAGIVPCAGANDGGGSIRIPAACCGLV
Mflv_1016|M.gilvum_PYR-GCK PARNPWDLTRSPGGSSGGSAAAVAAGITPCAGASDGGGSIRIPAACCGLV
MAB_2181c|M.abscessus_ATCC_199 ASRNPWDLGRTPGGSSGGSAAAVAAGIVPVAGASDGGGSIRIPAGCCGLV
MAV_0676|M.avium_104 PARNPWDTERTPGGSSGGSGAAVAAGIVPAAGANDGGGSIRIPAACNGLV
MMAR_3565|M.marinum_M PTRNPWDTSRTPGGSSGGSAAAVASGMVPFAHASDGGGSIRIPASCCGLV
MUL_2798|M.ulcerans_Agy99 PTRNPWDTSRTPGGSSGGSAAAVASGMVPFAHASDGGGSIRIPASCCGLV
Mb1294|M.bovis_AF2122/97 ATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSIRIPCTWCGLF
Rv1263|M.tuberculosis_H37Rv ATRNPWCLNRTPGGSSGGSAAAVAAGLAPVALGSDGGGSIRIPCTWCGLF
. **** :.*.**.**.* **:*:.* * ..*********. **.
MLBr_01596|M.leprae_Br4923 GLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYREAERIWHN--
TH_2520|M.thermoresistible__bu GLKPSRGRLPLDRHMRQMPLRIVANGVLTRSVRDTAAFYREAELLTGR--
MSMEG_6673|M.smegmatis_MC2_155 GLKPGRGLTPMGPALSESVHGATVQGVVSRTVRDTAAMLDVISGGEPSGP
Mvan_5822|M.vanbaalenii_PYR-1 GLKPGRGLTPMGPELGESMHGSAVQGVVSRTVRDTAAMLDVISGGEPWGP
Mflv_1016|M.gilvum_PYR-GCK GLKPGRGLTPSGPASGEAMHGAAVQGVVTRTVRDSAAMLDVISGGEPYGP
MAB_2181c|M.abscessus_ATCC_199 GLKPGRGITPMGPTAGEPMHGAAVNGTVSRTVRDTASMLDIIAGPEPTSP
MAV_0676|M.avium_104 GLKPSRGLAPYGPQTGELMLGMATQGVVTRTVRDSAALLDAIIGHHPDAS
MMAR_3565|M.marinum_M GLKPSQGRITLGPLR--EESGMSVEHCLSRTVRDTAALLDATRGPGVGDT
MUL_2798|M.ulcerans_Agy99 GLKPSQGRITLGPLR--EESGMSVEHCLSRTVRDTAALLDATRGPGVGDT
Mb1294|M.bovis_AF2122/97 GLKPQRDRISLEPHDG-AWQGLSVNGPIARSVMDAALLLDAT--------
Rv1263|M.tuberculosis_H37Rv GLKPQRDRISLEPHDG-AWQGLSVNGPIARSVMDAALLLDAT--------
**** :. . .: ::*:* *:* :
MLBr_01596|M.leprae_Br4923 --PKLP--PVGDVTQPGRQRLRIAVVTR-SVQRECSP--ELRELTLKSAR
TH_2520|M.thermoresistible__bu --TRLP--PIGDITRPGAERLRIAVCTR-SINRDASP--EVRELTLKTAA
MSMEG_6673|M.smegmatis_MC2_155 YAPKLPGTPFASAVGADPGKLRIGVRVPSAINPNPHR--EAYAAVEATAQ
Mvan_5822|M.vanbaalenii_PYR-1 FTPGMPNAPFASCVGQDPGRLRIGVRVPTAITPVPHR--EAFAAVEATVQ
Mflv_1016|M.gilvum_PYR-GCK FVPALPEASFASCVGTDPGTLRIGVRVPSAITPQPHR--EAFAAVEATTR
MAB_2181c|M.abscessus_ATCC_199 YLSAMPQDSYASQVGRDPGRLRIGVRVPTVITPNPDP--AAVAAVETAIR
MAV_0676|M.avium_104 YRVALPDKPFAQQITRPPGPLTIGYSARSALNPRPDR--EAVAAVENSAA
MMAR_3565|M.marinum_M VIAPPPIRPYTEELGADPGRLRIGILDHHPRGGPVDA--EVTHATQAVGR
MUL_2798|M.ulcerans_Agy99 VIAPPPIRPYTEELGADPGRLRIGILDHHPRGGPVDA--EVTHATQAVCR
Mb1294|M.bovis_AF2122/97 TTVPGPEGEFVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGA
Rv1263|M.tuberculosis_H37Rv TTVPGPEGEFVAAAARQPGRLRIALSTRVPTPLPVRCGKQELAAVHQAGA
* * *. .
MLBr_01596|M.leprae_Br4923 LLEELGHRVERVAEPPVPPN-FPDDFLLYWGLLAAMQVR--TGRLAFGNT
TH_2520|M.thermoresistible__bu LLEELGHHVTPIDN-PVPRQ-FQDDFLLYWAFLAMAVVR--SGRRTFGPG
MSMEG_6673|M.smegmatis_MC2_155 TLTELGHHVEELPQAPFDDAALAREFLLSWFVYTAWELE--EAKRISGAG
Mvan_5822|M.vanbaalenii_PYR-1 TLTDLGHHVDELPQAPFDDAALAREFLLTWFVYTAWELA--DAKRLTGAG
Mflv_1016|M.gilvum_PYR-GCK LLTDLGHEVTELAEAPYDDAVLARDFLLTWFVTLAWEVA--EAKRLTGAG
MAB_2181c|M.abscessus_ATCC_199 LLTHLGHEVEEVS-APYDDGQLARDFLLTWFVHAAYEVD--YTKRLTGAG
MAV_0676|M.avium_104 LLSALGHRVREVA-PPYDDAALGRDFLTIWFAQLHQQVA--DIRRRTGAR
MMAR_3565|M.marinum_M LLESLGHHVDVAWPAALEDASFDSTFGALWSTNMGLAQRRFEEQLGRPLG
MUL_2798|M.ulcerans_Agy99 LLESLGHHVEVAWPAALEDASFDSTFGALWSTNMGLAQRRFEEQLGRPLG
Mb1294|M.bovis_AF2122/97 LLRDLGHDVVVRDPDYPAST---------YANYLPRFFRGISDDADAQAH
Rv1263|M.tuberculosis_H37Rv LLRDLGHDVVVRDPDYPAST---------YANYLPRFFRGISDDADAQAH
* *** * :
MLBr_01596|M.leprae_Br4923 FDRTKLDSLTLGLDRHASRNMHRLPKAIMRLRRLRRRTADFFATYDVLLT
TH_2520|M.thermoresistible__bu FDRDRLDNLTLGLDSHAARNLHRLPAAMVRLSRLRRVTARLTRDFDVVLT
MSMEG_6673|M.smegmatis_MC2_155 DEAFERDTLILAAIGRATSSVAYVDAVQRRHDHTRRLTT-FFESYDLLMT
Mvan_5822|M.vanbaalenii_PYR-1 DDAFERDTLILAAIGRATSSVDYLDAVQRRHDHTRRLTR-FFDSYDLLLT
Mflv_1016|M.gilvum_PYR-GCK DDQFETDTLVMAALGRATSSVDYVDAVQRRHEHTRRLTR-FFESYDLLLT
MAB_2181c|M.abscessus_ATCC_199 DEGFERDTLLIAALGRTTSGPEYVAAVERRHDYVRELAT-FFGDYDLLLT
MAV_0676|M.avium_104 DNDFEADTLAISELGRSGGVLPVMSALENRNTYIQSLAA-FHEGYDFFLT
MMAR_3565|M.marinum_M EDEFELVNRAQADFAKHFTSVDYALALSAIAAFRRAIQSWWHDGWDLLLT
MUL_2798|M.ulcerans_Agy99 EDEFELVNRAQADFAKHFTSVDYALALSAIAAFRRAIQSWWHDGWDLLLT
Mb1294|M.bovis_AF2122/97 PDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDD-VDVVVT
Rv1263|M.tuberculosis_H37Rv PDRLEARTRAIARLGSFFSDRRMAALRAAEVVLSSRIQSIFDD-VDVVVT
: . . . *..:*
MLBr_01596|M.leprae_Br4923 PTVADETPRIGYLTPT-DYQQV----------------------MDRLMG
TH_2520|M.thermoresistible__bu PTLADVPPRIGHLDPTADYQQI----------------------IDRLVD
MSMEG_6673|M.smegmatis_MC2_155 PTLATPPPRIGEFELPELLKRGSDVLLKTRTAGLLRFTKILDDSVDANLA
Mvan_5822|M.vanbaalenii_PYR-1 PSLATPPPAIGEFDLPVALQKASDVLLKTRTAHLLRFTKIVDDIVDKNLG
Mflv_1016|M.gilvum_PYR-GCK PTLATPPPRIGEFDMPAVMQRAADVLLKTRTARLLRHTGIVDDMVDQNLG
MAB_2181c|M.abscessus_ATCC_199 PTLAKAPPRIGEFNLPAPLAAVSDLLLRTKTARVLKYANIVDSMINDNLG
MAV_0676|M.avium_104 PTLATPPLVVGATSTSAALQRAARLVSRVRGGKLMRLSGILDDLIRESLG
MMAR_3565|M.marinum_M PTVASLPVKLGTIVSTPEHPMAA---------------------MQRAGE
MUL_2798|M.ulcerans_Agy99 PTVASLPVKLGTIVSTPEHPMAA---------------------MQRAGE
Mb1294|M.bovis_AF2122/97 PGAATGPSRIG--AYQRRGAVST---------------------LLLVVQ
Rv1263|M.tuberculosis_H37Rv PGAATGPSRIG--AYQRRGAVST---------------------LLLVVQ
* * . :* :
MLBr_01596|M.leprae_Br4923 WVAFTPLQNVTGEPAISLPLAQSADGMPVGMMFTADFGQEAQLLELAFEL
TH_2520|M.thermoresistible__bu WVAFTPLHNVTGEPAVSLPLAQSRDGLPVGMMFAAALGEEARLLRLAYEL
MSMEG_6673|M.smegmatis_MC2_155 WVPYTQLANITGRPAVTLPLHWTADGLPLGVQFVAPLEGESLLIRLGAQL
Mvan_5822|M.vanbaalenii_PYR-1 WVPYTQLANITGRPAISLPLHWTEEGLPLGVQFVAPLGGESLLVRLAAQL
Mflv_1016|M.gilvum_PYR-GCK WVPYTQLANITGRPAISLPLHWTADGLPLGVQFVAPLAGEALLLRLAGQL
MAB_2181c|M.abscessus_ATCC_199 WVPYTQLANLTGRPAISLPLYQTPKGLPLGVQFVAPLGGESLLIRLAAQL
MAV_0676|M.avium_104 WVPYTQLANLTGRPAISVPLHWTDDGLPLGVQFVGKLGADGDLLQLAAQL
MMAR_3565|M.marinum_M FVPFTPPFNTSGQPAISLPLEWTPEGLPLGVQLVAAYGREDVLLRIASQL
MUL_2798|M.ulcerans_Agy99 FVPFTPPFNTSGQPAISLPLEWTPEGLPLGVQLVAAYGREDVLLRIASQL
Mb1294|M.bovis_AF2122/97 RVPYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQI
Rv1263|M.tuberculosis_H37Rv RVPYFQVWNLTGQPAAVVPWDFDGDGLPMSVQLVGRPYDEATLLALAAQI
*.: * :*.** :* .*:*:.: :.. : *: :. ::
MLBr_01596|M.leprae_Br4923 EEARPWARIQIGD-----
TH_2520|M.thermoresistible__bu EEARPWPRIDQRS-----
MSMEG_6673|M.smegmatis_MC2_155 EEAMPWKDRVAPV-----
Mvan_5822|M.vanbaalenii_PYR-1 EQAMPWRDRVAPV-----
Mflv_1016|M.gilvum_PYR-GCK EQAAPWAHRTAAL-----
MAB_2181c|M.abscessus_ATCC_199 EATEPWAHRRPTL-----
MAV_0676|M.avium_104 EQARPWAQRHPAPVTPPN
MMAR_3565|M.marinum_M EQAKPWAQRTPDI-----
MUL_2798|M.ulcerans_Agy99 EQAKPWAQRTPDI-----
Mb1294|M.bovis_AF2122/97 ESARPWAHRRPSVS----
Rv1263|M.tuberculosis_H37Rv ESARPWAHRRPSVS----
* : **