For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
MTELCHLSAHELVGLMSTGSVSCREVIQQHLARIRAVNPALNALVEADDPERCLRQADHADECVARGAPL GAAHGLPVVIKDVMQVAGLACSGGSPGLRAVADTDATVVSRLRAQGAIVLGMTNVPEMSRGGESNNNLYG RTNNPYDLTRTPGGSSGGSAALVAAGGAALSVGSDGGGSIRQPCHNTGIAGLKPTHGRIPRTGSVFGDAL GVFSPFVCYGPLARSVRDLFLGLSIMNGPDLADPYTAPAPLGSPDDVELGTLRVAAYLDDGISPPSEEVT AVVTAAVEALREVVAVIDHAVPPCLNRTTDLLWTSIFLGGDRGRGFEEDLGAIGTTAPSEELAEFTAQAR QVDFSLTEARRRLVDIDHYRIQMLAFMADYDVIIGPAMPTPAKPHHHGLVEISDFSHLMVHNLTGWPAAV VRCGTSPGGLPIGVQIAARPWHDGTALAVASHLEAIFGGWQAPGLLVT
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. marinum M | MMAR_2765 | - | - | 100% (468) | amidase |
| M. marinum M | MMAR_3565 | amiC_1 | 4e-46 | 33.12% (477) | amidase AmiC_1 |
| M. marinum M | MMAR_4176 | amiB2 | 2e-40 | 32.30% (483) | amidase AmiB2 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3200 | - | 9e-52 | 31.46% (499) | amidase |
| M. gilvum PYR-GCK | Mflv_2712 | - | 1e-41 | 31.75% (463) | amidase |
| M. tuberculosis H37Rv | Rv3175 | - | 1e-51 | 31.46% (499) | amidase |
| M. leprae Br4923 | MLBr_01702 | gatA | 7e-36 | 30.08% (502) | aspartyl/glutamyl-tRNA amidotransferase subunit A |
| M. abscessus ATCC 19977 | MAB_4643c | - | 9e-46 | 31.60% (462) | amidase family protein |
| M. avium 104 | MAV_1414 | - | 3e-41 | 31.60% (481) | amidase |
| M. smegmatis MC2 155 | MSMEG_3970 | - | 1e-65 | 36.97% (476) | glutamyl-tRNA(Gln) amidotransferase subunit A |
| M. thermoresistible (build 8) | TH_4482 | - | 6e-40 | 29.98% (477) | PUTATIVE Amidase |
| M. ulcerans Agy99 | MUL_2798 | amiC_1 | 8e-46 | 33.12% (477) | amidase AmiC_1 |
| M. vanbaalenii PYR-1 | Mvan_3824 | - | 1e-37 | 29.72% (461) | amidase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3200|M.bovis_AF2122/97 MAMSAKASDDIA-------------WLPATAQLAVLAAKKVSSAELVELY
Rv3175|M.tuberculosis_H37Rv MAMSAKASDDIA-------------WLPATAQLAVLAAKKVSSAELVELY
MMAR_2765|M.marinum_M -------MTELC-------------HLSAHELVGLMSTGSVSCREVIQQH
MSMEG_3970|M.smegmatis_MC2_155 ------MSTDII-------------YSDATGLAELIRSRQLSPVEVVQAH
Mflv_2712|M.gilvum_PYR-GCK -------MPDSS-ARSTSRNRFPDRFPTVANQLFQLASGTATSVDLVRRS
Mvan_3824|M.vanbaalenii_PYR-1 -------MADTAYSRITSRR----RFPTLGNQLFQLASGTATSVDLVSRS
TH_4482|M.thermoresistible__bu -------MVDAYKPPAK--------LPTLTDLLYQLASGAVTSQELVRRS
MAV_1414|M.avium_104 -------MNDLA-------------FAGAAAQARMLANGDLSAPELLEVY
MUL_2798|M.ulcerans_Agy99 -------MSTLAEQTR---------WMDATDQAALVVAGEASPSELLEAA
MAB_4643c|M.abscessus_ATCC_199 -------------------------------------------MEVADAV
MLBr_01702|M.leprae_Br4923 ---MIGSLHEIIR-------------LDASTLAAKIVAKELSSVEITQAC
::
Mb3200|M.bovis_AF2122/97 LSRIDTYNASLNAIVTVD-PDAARRVAKRSDAARARGD--ELGPLHGLPI
Rv3175|M.tuberculosis_H37Rv LSRIDTYNASLNAIVTVD-PDAARRVAKRSDAARARGD--ELGPLHGLPI
MMAR_2765|M.marinum_M LARIRAVNPALNALVEADDPERCLRQADHADECVARGA--PLGAAHGLPV
MSMEG_3970|M.smegmatis_MC2_155 LDRIEAVNPKINAIVTVA--ERALAQARSAEAAVMRGD--DLPPLHGVPF
Mflv_2712|M.gilvum_PYR-GCK LVAIEASQPTLNAFRVVL-AERALADAAEADRLRAAGHDRARRPLLGIPI
Mvan_3824|M.vanbaalenii_PYR-1 LQAIEASQPTLNAFRVVL-TEQALADAAEADRKRAAGADLSRHPLLGVPI
TH_4482|M.thermoresistible__bu LRAIEASQSTLNAFRVVL-ADRALAEAAEADRKRAAG---EHLPLLGVPI
MAV_1414|M.avium_104 LERIARLDSQLRCYRVVL-ADRARREAAAAQSRLDAG---ERLPLLGVPV
MUL_2798|M.ulcerans_Agy99 IERIERINPALNAVVIRW-FDHARETASADLP---------GGPFRGVPF
MAB_4643c|M.abscessus_ATCC_199 LRRIEKVNPLLNAFVFHN-PEQVLRDARRLTEDLTKRT--TLPPLYGIPY
MLBr_01702|M.leprae_Br4923 LDQIEATDDTYRAFLHVA-AEKALSAAAAVDKAVAAGG-QLSSTLAGVPL
: * : .. : * . *:*
Mb3200|M.bovis_AF2122/97 TVKDSYETAG---MRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTN
Rv3175|M.tuberculosis_H37Rv TVKDSYETAG---MRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTN
MMAR_2765|M.marinum_M VIKDVMQVAG---LACSGGSPGLR-AVADTDATVVSRLRAQGAIVLGMTN
MSMEG_3970|M.smegmatis_MC2_155 TVKDSIDTAD---VLTQRGSPIFAGRIPQTDAVSVARLKAAGAIVLAKTN
Mflv_2712|M.gilvum_PYR-GCK AVKDDVDVAG----VPTRFGASGEVPEAVADAEVVRRLRAAGAVIVGKTN
Mvan_3824|M.vanbaalenii_PYR-1 AVKDDVDVAG----VPTRFGADGEVPPAEADAEVVRRLRAAGAVIVGKTN
TH_4482|M.thermoresistible__bu AVKDDVDIAG----VPTRFGAVGDVPPATEDAEVVRRLRAAGAVIVGKTH
MAV_1414|M.avium_104 AIKDDVDVAG----EVTTFGSGGHGPAVSSDAEVVRRLRAAGAVIIGKTN
MUL_2798|M.ulcerans_Agy99 LIKDLYALYAGQPISNGNLAFKQAHVIADADTTLVGRYRAAGLVIAGRTN
MAB_4643c|M.abscessus_ATCC_199 SVKEVCAVAAT---PVTSGVVAFKDMVADRDEPVSARLRASGGLFLGKTN
MLBr_01702|M.leprae_Br4923 ALKDVFTTVD---MPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTN
:*: * * : * . . *:
Mb3200|M.bovis_AF2122/97 MPTGNQDVQASNPVFGRTNNPWDAARTSGGSAGGGAAATAAGLTSFDYGS
Rv3175|M.tuberculosis_H37Rv MPTGNQDVQASNPVFGRTNNPWDAARTSGGSAGGGAAATAAGLTSFDYGS
MMAR_2765|M.marinum_M VPEMSRGGESNNNLYGRTNNPYDLTRTPGGSSGGSAALVAAGGAALSVGS
MSMEG_3970|M.smegmatis_MC2_155 LPEFSYSTETDNLLTGRSNNPWNLDRTPGGSSGGESAAIAAGLSPLGLGT
Mflv_2712|M.gilvum_PYR-GCK TCELGQWPFTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGS
Mvan_3824|M.vanbaalenii_PYR-1 TCELGQWPFTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGS
TH_4482|M.thermoresistible__bu TCELGQWPFTSGPAFGHTRNPWSPRHTPGGSSGGSAAAVAAGLVTAAIGS
MAV_1414|M.avium_104 VPELMMMPYTESLTFGATRNPWDPRRTPGGSSGGSAAAVAAGLAPLALGS
MUL_2798|M.ulcerans_Agy99 SPELGSLPTTEPVAWGPTRNPWDTSRTPGGSSGGSAAAVASGMVPFAHAS
MAB_4643c|M.abscessus_ATCC_199 IAEGGYKASSDNHLYGSTRNPWHPGMTAGGSSSGAGAAVAAGMGQLAQGT
MLBr_01702|M.leprae_Br4923 MDEFAMGSSTENSAYGPTRNPWNVDRVPGGSGGGSAAALAAFQAPLAIGS
:. * :.**: ..***..* .* *: .:
Mb3200|M.bovis_AF2122/97 EIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAG
Rv3175|M.tuberculosis_H37Rv EIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAG
MMAR_2765|M.marinum_M DGGGSIRQPCHNTGIAGLKPTHGRIPRTGSVFGDALGV----FSPFVCYG
MSMEG_3970|M.smegmatis_MC2_155 DLAISVRGPAAQTGIAALKATHGRIPMTG-VWPRVP-------RRFWHVG
Mflv_2712|M.gilvum_PYR-GCK DGAGSVRIPAAWTHLVGIKPQRGRISTSP---------LPEAFNGITVNG
Mvan_3824|M.vanbaalenii_PYR-1 DGAGSVRIPAAWTHLVGIKPQRGRISTWP---------LTEAFNGITVHG
TH_4482|M.thermoresistible__bu DGAGSVRIPAAWTHLVGIKPQRGRISTWP---------LPEAFNGITVHG
MAV_1414|M.avium_104 DGGGSIRIPSTWCGLFGLKPQRDRVSLEP---------HDDAWYGLSVNG
MUL_2798|M.ulcerans_Agy99 DGGGSIRIPASCCGLVGLKPSQGRITLGP---------LREES-GMSVEH
MAB_4643c|M.abscessus_ATCC_199 DGGGSIRIPAALNGVVGFKPSLGRIPQTR---------LAGRFHTFAFHG
MLBr_01702|M.leprae_Br4923 DTGGSIRQPAALTATVGVKPTYGTVSRYG---------LVACASSLDQGG
: . * * *. . *. :. :
Mb3200|M.bovis_AF2122/97 VQVRGARDIIPALEATVGPMRADG----GFSYALAPPRAG----ALKDFR
Rv3175|M.tuberculosis_H37Rv VQVRGARDIIPALEATVGPMRADG----GFSYALAPPRAG----ALKDFR
MMAR_2765|M.marinum_M PLARSVRDLFLGLSIMNGPDLADPY---TAPAPLGSPDDV----ELGTLR
MSMEG_3970|M.smegmatis_MC2_155 PMARTVRDLALAYELLAGPDGADAF---AISPVRFDAGTA----AVDSLR
Mflv_2712|M.gilvum_PYR-GCK VLARTVSDAAMVLDAAAGNAAGD--LHTPAPVTVSDFVSR----APGPLR
Mvan_3824|M.vanbaalenii_PYR-1 VLARTVSDAALLLDAASGNAPGE--LHTPPPVRVSDYVSR----APGPLR
TH_4482|M.thermoresistible__bu VLARTVTDAALVLDAASGNAPGD--LHQPPPVRVSDYVGQ----APGPLR
MAV_1414|M.avium_104 PIARTVIDAAVFLDATS-TVPG-------PEGEFVAAATR----EPGRLR
MUL_2798|M.ulcerans_Agy99 CLSRTVRDTAALLDATRGPGVGDTVIAPPPIRPYTEELGA----DPGRLR
MAB_4643c|M.abscessus_ATCC_199 PITRTVADAALMLTTMAGFDDEDPLSLPSDGVDYLAALDQ----PIESGR
MLBr_01702|M.leprae_Br4923 PCARTVLDTALLHAVIAGHDARDSTSVEAAVPDIVGAAKAGESGDLHGVR
* . * *
Mb3200|M.bovis_AF2122/97 VAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAVSHN
Rv3175|M.tuberculosis_H37Rv VAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAVSHN
MMAR_2765|M.marinum_M VAAYLDDGISPPSEEVTAVVTAAVEALREVVAVIDHA-----VPPCLNRT
MSMEG_3970|M.smegmatis_MC2_155 VGWLVEPGFGPIDAEVAATVRAAAEALSSIGLAVEPV-----RIPALERD
Mflv_2712|M.gilvum_PYR-GCK VAVSTRFPFTGFRATLHPEIRSALDGVADQLSQLGHT-----VLNADPDY
Mvan_3824|M.vanbaalenii_PYR-1 IAMSTKFPFTVFRAGLHPEIRTALQNVADQLTQLGHT-----IVAADPDY
TH_4482|M.thermoresistible__bu IAVSTKFPFTLFRAKLHPEIRAAVQTVADQLEQLGHT-----VFPADPEY
MAV_1414|M.avium_104 IALSTKVPTPLPVRVGKPEL-AAVQQAGALLRDLGHD-----VVTADPEY
MUL_2798|M.ulcerans_Agy99 IGILDHHPR---GGPVDAEVTHATQAVCRLLESLGHH-----VEVAWPAA
MAB_4643c|M.abscessus_ATCC_199 MAWSPDLGISKCDSEIQGICEEALIAFVEMGWTVEED-------RPDWTD
MLBr_01702|M.leprae_Br4923 VGVVRQLRGEGYQSGVLASFQAAVEQLIALGATVSEVDCPHFDYALAAYY
:. * :
Mb3200|M.bovis_AF2122/97 IFQSLVFGAFAVDRSTL------------SPASAAALGLRAVRHPRGEAA
Rv3175|M.tuberculosis_H37Rv IFQSLVFGAFAVDRSTL------------SPASAAALGLRAVRHPRGEAA
MMAR_2765|M.marinum_M TDLLWTSIFLGGDRGRG------------FEEDLGAIGTTAPSEELAEFT
MSMEG_3970|M.smegmatis_MC2_155 FALDVFMRLHVMELKPA------------FAEATAGRGD---EELFAISK
Mflv_2712|M.gilvum_PYR-GCK -TLGMSWNFLARSTSG-------------IPEWMDRLGHRVTWDSRTRSN
Mvan_3824|M.vanbaalenii_PYR-1 -HLRMSWNFLSRSTSG-------------IPEWMDRLGRGVNWDSRTRSN
TH_4482|M.thermoresistible__bu -TVGMSWSFLSRSTAG-------------LLDWSRRLGDNVLWDKRTVAN
MAV_1414|M.avium_104 PAAAILGNYLPRYLRG-------------ICDDADSLAHPERLEARTRNM
MUL_2798|M.ulcerans_Agy99 LEDASFDSTFGALWSTNMG-----LAQRRFEEQLGRPLGEDEFELVNRAQ
MAB_4643c|M.abscessus_ATCC_199 PAKTMWEGVWGPVYAG--------------KLDAANWDDQAGHVDQELVQ
MLBr_01702|M.leprae_Br4923 LILPSEVSSNLARFDAMRYGLRVGDDGSRSAEEVIALTRAAGFGPEVKRR
Mb3200|M.bovis_AF2122/97 NALGATLQSH----RAWLFADAARHEMRDRWAGFFNEFDVLLLPVTPTPA
Rv3175|M.tuberculosis_H37Rv NALGATLQSH----RAWLFADAARHEMRDRWAGFFNEFDVLLLPVTPTPA
MMAR_2765|M.marinum_M AQARQVDFSL----TEARRRLVDIDHYRIQMLAFMADYDVIIGPAMPTPA
MSMEG_3970|M.smegmatis_MC2_155 RILATPDTSI----QDFVEAEQAGERLRDGFADYFSRYDALLAPVLPVPA
Mflv_2712|M.gilvum_PYR-GCK ARMGRLLSQH----TLRKARAREAAAQQRIG-WIFNLADVVVAPTTAQPP
Mvan_3824|M.vanbaalenii_PYR-1 ARMGWLLSQN----VLRKARAGEAASQQRIG-WIFNLADVVLAPTTALPP
TH_4482|M.thermoresistible__bu MRLGRLLSES----ALRKARAREAAAQRRMS-WIFNIADVVLAPTTALPP
MAV_1414|M.avium_104 ARMGSFFSDR----RMAAIRAGEGPLIARIQ-AIFDDVDVVVTPGTAAGP
MUL_2798|M.ulcerans_Agy99 ADFAKHFTSV----DYALALSAIAAFRRAIQSWWHDGWDLLLTPTVASLP
MAB_4643c|M.abscessus_ATCC_199 VIRDGARLTT----VQVQRADAARGKMVDTLREFLRRFDFLVTPVTTAQA
MLBr_01702|M.leprae_Br4923 IMIGAYALSAGYYDSYYSQAQKVRTLIARDLDKAYRSVDVLVSPATPTTA
* :: * . .
Mb3200|M.bovis_AF2122/97 PLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTA
Rv3175|M.tuberculosis_H37Rv PLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTA
MMAR_2765|M.marinum_M KPHH-------HGLVEIS----DFSHLM----VHNLTGWPAAVVRCGTSP
MSMEG_3970|M.smegmatis_MC2_155 HEHR-------AKEFDINGQTVDATYIMGATVPFNVTGLPALSLRFGTSS
Mflv_2712|M.gilvum_PYR-GCK PEAH----AFDGL--GGLATDRMMIRACPVTWPWNLLGWPSINVPAGFTS
Mvan_3824|M.vanbaalenii_PYR-1 PEVR----AFDDL--GGLATDRMMIKACPVTWPWNLLGWPSINVPAGFTS
TH_4482|M.thermoresistible__bu PEVH----AFDQL--GGLATDRLMIKACPVTWPWNLLGWPSINVPAGFTS
MAV_1414|M.avium_104 SRVG----AYQRR--GAVSTLLRVGQCVPYQQIWNLTGQPAAVVPWDFDA
MUL_2798|M.ulcerans_Agy99 VKLG----TIVSTPEHPMAAMQRAGEFVPFTPPFNTSGQPAISLPLEWTP
MAB_4643c|M.abscessus_ATCC_199 FGHD-----EFCPAVLAGAPLLERLMGWVLTYPFNMTTNPAISIPVGFTR
MLBr_01702|M.leprae_Br4923 FSLG-----------EKADDPLAMYLFDLCTLPLNLAGHCGMSVPSGLSS
* . :
Mb3200|M.bovis_AF2122/97 TG-LPIGIQAMGPAGGDRTTVEFAALLTEVLGG-FRVPPL----------
Rv3175|M.tuberculosis_H37Rv TG-LPIGIQAMGPAGGDRTTVEFAALLTEVLGG-FRVPPL----------
MMAR_2765|M.marinum_M GG-LPIGVQIAARPWHDGTALAVASHLEAIFGG-WQAPGLLVT-------
MSMEG_3970|M.smegmatis_MC2_155 AG-LPINVQLISTWLAESTVLNLASLLESVSPVRNQHPGI----------
Mflv_2712|M.gilvum_PYR-GCK DG-LPIGVQLMGPADSEPLLISLAAALEAINGWATLQPENWWAPQPPTEP
Mvan_3824|M.vanbaalenii_PYR-1 DG-LPIGVQLMGPANSEPLLVSLAAALEAINGWAVHQPEDWWTPQPPPEP
TH_4482|M.thermoresistible__bu DG-LPIGVQLMGPANSEPLLVSLAAALEGINGWAARQPRPWWLT-PPAAP
MAV_1414|M.avium_104 DG-LPMSVQLVGRPYDEATLLSLSAQIEAARPWALRRPPVC---------
MUL_2798|M.ulcerans_Agy99 EG-LPLGVQLVAAYGREDVLLRIASQLEQAKPWAQRTPDI----------
MAB_4643c|M.abscessus_ATCC_199 NG-VPVGLQIVGRLRADADVLRLAAAFEAVRPWAQRRPSDAVLNGENP--
MLBr_01702|M.leprae_Br4923 DDDLPVGLQIMAPALADDRLYRVGAAYEAARGPLPSAI------------
. :*:.:* . : ..:
Mb3200|M.bovis_AF2122/97 -----------------
Rv3175|M.tuberculosis_H37Rv -----------------
MMAR_2765|M.marinum_M -----------------
MSMEG_3970|M.smegmatis_MC2_155 -----------------
Mflv_2712|M.gilvum_PYR-GCK DL----TAPDSVTGQVA
Mvan_3824|M.vanbaalenii_PYR-1 DLGSNVAAPASVEGQVA
TH_4482|M.thermoresistible__bu XX----XASAAAS----
MAV_1414|M.avium_104 -----------------
MUL_2798|M.ulcerans_Agy99 -----------------
MAB_4643c|M.abscessus_ATCC_199 -----------------
MLBr_01702|M.leprae_Br4923 -----------------