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EVADAVLRRIEKVNPLLNAFVFHNPEQVLRDARRLTEDLTKRTTLPPLYGIPYSVKEVCAVAATPVTSGV VAFKDMVADRDEPVSARLRASGGLFLGKTNIAEGGYKASSDNHLYGSTRNPWHPGMTAGGSSSGAGAAVA AGMGQLAQGTDGGGSIRIPAALNGVVGFKPSLGRIPQTRLAGRFHTFAFHGPITRTVADAALMLTTMAGF DDEDPLSLPSDGVDYLAALDQPIESGRMAWSPDLGISKCDSEIQGICEEALIAFVEMGWTVEEDRPDWTD PAKTMWEGVWGPVYAGKLDAANWDDQAGHVDQELVQVIRDGARLTTVQVQRADAARGKMVDTLREFLRRF DFLVTPVTTAQAFGHDEFCPAVLAGAPLLERLMGWVLTYPFNMTTNPAISIPVGFTRNGVPVGLQIVGRL RADADVLRLAAAFEAVRPWAQRRPSDAVLNGENP*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. abscessus ATCC 19977 | MAB_4643c | - | - | 100% (455) | amidase family protein |
| M. abscessus ATCC 19977 | MAB_4415 | - | 2e-59 | 36.12% (443) | putative amidase |
| M. abscessus ATCC 19977 | MAB_1674c | - | 1e-48 | 31.90% (442) | amidase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3409 | amiD | 7e-52 | 31.58% (456) | amidase AmiD |
| M. gilvum PYR-GCK | Mflv_2712 | - | 7e-47 | 31.94% (454) | amidase |
| M. tuberculosis H37Rv | Rv3375 | amiD | 7e-52 | 31.58% (456) | amidase AmiD |
| M. leprae Br4923 | MLBr_01596 | - | 8e-40 | 29.39% (456) | amidase |
| M. marinum M | MMAR_3673 | amiA2 | 8e-47 | 32.61% (463) | amidase AmiA2 |
| M. avium 104 | MAV_0676 | - | 2e-47 | 31.20% (468) | 6-aminohexanoate-cyclic-dimer hydrolase |
| M. smegmatis MC2 155 | MSMEG_2497 | - | 3e-62 | 33.70% (457) | putative amidase |
| M. thermoresistible (build 8) | TH_4482 | - | 2e-47 | 31.71% (451) | PUTATIVE Amidase |
| M. ulcerans Agy99 | MUL_3616 | amiA2 | 2e-47 | 32.14% (473) | amidase |
| M. vanbaalenii PYR-1 | Mvan_3824 | - | 6e-47 | 31.03% (464) | amidase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2712|M.gilvum_PYR-GCK MP-DSS-ARSTSRNRFPDRFPTVANQLFQLASGTATSVDLVRRSLVAIEA
Mvan_3824|M.vanbaalenii_PYR-1 MA-DTAYSRITSRR----RFPTLGNQLFQLASGTATSVDLVSRSLQAIEA
TH_4482|M.thermoresistible__bu MV-DAYKPPAK--------LPTLTDLLYQLASGAVTSQELVRRSLRAIEA
MMAR_3673|M.marinum_M MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
MUL_3616|M.ulcerans_Agy99 MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
Mb3409|M.bovis_AF2122/97 -MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIER
Rv3375|M.tuberculosis_H37Rv -MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIER
MAB_4643c|M.abscessus_ATCC_199 -------------------------------------MEVADAVLRRIEK
MSMEG_2497|M.smegmatis_MC2_155 ------------------MIPTSTDLVEAYRRKEMSPVEATQAALDAIDS
MLBr_01596|M.leprae_Br4923 ------MQRVHAFGDDALGDLDAVGLADAIRAGWVSRADVIEAAIVRTEA
MAV_0676|M.avium_104 ------------------MALDATALAGLVADKQVTPAELLALARQRADA
: :
Mflv_2712|M.gilvum_PYR-GCK SQPTLNAFRVVLAERALADAAEADRLRAAGHDRARRPLLGIPIAVKDD-V
Mvan_3824|M.vanbaalenii_PYR-1 SQPTLNAFRVVLTEQALADAAEADRKRAAGADLSRHPLLGVPIAVKDD-V
TH_4482|M.thermoresistible__bu SQSTLNAFRVVLADRALAEAAEADRKRAAG---EHLPLLGVPIAVKDD-V
MMAR_3673|M.marinum_M SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDD-V
MUL_3616|M.ulcerans_Agy99 SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDD-V
Mb3409|M.bovis_AF2122/97 LDPQLKSYAFVMPETALAAARAADADIARGH--YEGVLHGVPIGVKDL-C
Rv3375|M.tuberculosis_H37Rv LDPQLKSYAFVMPETALAAARAADADIARGH--YEGVLHGVPIGVKDL-C
MAB_4643c|M.abscessus_ATCC_199 VNPLLNAFVFHNPEQVLRDARRLTEDLTKRT--TLPPLYGIPYSVKEV-C
MSMEG_2497|M.smegmatis_MC2_155 YDKSVNAFVLVDHEGALAAAKESEARWFSGE--QLGPADGVPTSIKDA-L
MLBr_01596|M.leprae_Br4923 VNPALGGLAYEAFQWA-RQTASKAGS---------GFFSGVPTFIKDN-I
MAV_0676|M.avium_104 VNPRLNAIVCRLDDVADRQAADPNLS---------GPFAGVPFLLKDLGQ
: : . : . : *:* :*:
Mflv_2712|M.gilvum_PYR-GCK DVAGVPTRFG--ASGEVPEAVADAEVVRRLRAAGAVIVGKTNTCELGQWP
Mvan_3824|M.vanbaalenii_PYR-1 DVAGVPTRFG--ADGEVPPAEADAEVVRRLRAAGAVIVGKTNTCELGQWP
TH_4482|M.thermoresistible__bu DIAGVPTRFG--AVGDVPPATEDAEVVRRLRAAGAVIVGKTHTCELGQWP
MMAR_3673|M.marinum_M DVAGVPTAFG--TQGYVRPAAHDSEVVRRLKAAGAVIIGKTNTCELGQWP
MUL_3616|M.ulcerans_Agy99 DVAGVPTAFG--TQGYVQPAAHDSEVVRRLKAAGAVIIGKTNTCELGQWP
Mb3409|M.bovis_AF2122/97 YTVDAPTAAGTTIFRDFRP-AYDATVVARLRAAGAVIIGKLAMTEGAYLG
Rv3375|M.tuberculosis_H37Rv YTVDAPTAAGTTIFRDFRP-AYDATVVARLRAAGAVIIGKLAMTEGAYLG
MAB_4643c|M.abscessus_ATCC_199 AVAATPVTSGVVAFKDMVA-DRDEPVSARLRASGGLFLGKTNIAEGGYKA
MSMEG_2497|M.smegmatis_MC2_155 WTRGWPTLRGSTLIDEAGPWDEDAPSVARLREAGAVIIGKTTTPEYSWKG
MLBr_01596|M.leprae_Br4923 DVAGQPTMRGS-DAWVPRNAFDDGEFTRLYLATGPVSLGKTQLSEFGFSA
MAV_0676|M.avium_104 EYRGFPTSCGS-RSLANDVADRHALVTQRFLDAGLVIFGKTNTPEFGAKG
*. * . :* : .** * .
Mflv_2712|M.gilvum_PYR-GCK FTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGSDGAGSVRI
Mvan_3824|M.vanbaalenii_PYR-1 FTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGSDGAGSVRI
TH_4482|M.thermoresistible__bu FTSGPAFGHTRNPWSPRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSVRI
MMAR_3673|M.marinum_M FTSGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRV
MUL_3616|M.ulcerans_Agy99 FTSGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRV
Mb3409|M.bovis_AF2122/97 --YHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRF
Rv3375|M.tuberculosis_H37Rv --YHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRF
MAB_4643c|M.abscessus_ATCC_199 SSDNHLYGSTRNPWHPGMTAGGSSSGAGAAVAAGMGQLAQGTDGGGSIRI
MSMEG_2497|M.smegmatis_MC2_155 VTDSYRYGATVNPWDTTKTAGGSSGGSAAAVGLGMGPWSVGTDGGGSVRI
MLBr_01596|M.leprae_Br4923 SAEHMRLGPVRNPWDTDYTAGASSSGSGAFVAAGVVPMAHANDGGGSIRI
MAV_0676|M.avium_104 VTEPDYWGPARNPWDTERTPGGSSGGSGAAVAAGIVPAAGANDGGGSIRI
*** .* **.*... .. *: : ..* .**:*.
Mflv_2712|M.gilvum_PYR-GCK PAAWTHLVGIKPQRGRISTSPLPEAFNG-ITVNGVLARTVSDAAMVLDAA
Mvan_3824|M.vanbaalenii_PYR-1 PAAWTHLVGIKPQRGRISTWPLTEAFNG-ITVHGVLARTVSDAALLLDAA
TH_4482|M.thermoresistible__bu PAAWTHLVGIKPQRGRISTWPLPEAFNG-ITVHGVLARTVTDAALVLDAA
MMAR_3673|M.marinum_M PAAWTHLVGIKPQRGRISTWPWPETFNG-ITVNGVLARTVADAALVLDAA
MUL_3616|M.ulcerans_Agy99 PAAWTHLVGIKPQRGRISTWPWPETFNG-ITVNGVLARTVADAALVLDAA
Mb3409|M.bovis_AF2122/97 PTSMCGVTGIKPTWGRVSRHGVVELAAS-YDHVGPITRSAHDAAVLLSVI
Rv3375|M.tuberculosis_H37Rv PTSMCGVTGIKPTWGRVSRHGVVELAAS-YDHVGPITRSAHDAAVLLSVI
MAB_4643c|M.abscessus_ATCC_199 PAALNGVVGFKPSLGRIPQTRLAGRFHT-FAFHGPITRTVADAALMLTTM
MSMEG_2497|M.smegmatis_MC2_155 PGGFTGTVALKPTYGLVPLY-PMSAFGS-LAHVGPMTRSVRDCAALLDVI
MLBr_01596|M.leprae_Br4923 PASCNGLVGLKPSRGRLPLDSELRRLPVGIVVNGVLTRSVRDTAAFYR--
MAV_0676|M.avium_104 PAACNGLVGLKPSRGLAPYGPQTGELMLGMATQGVVTRTVRDSAALLDAI
* . ..:** * . * ::*:. * * .
Mflv_2712|M.gilvum_PYR-GCK AGNAAGDLHTPAPVTVSDFVSRAPGPLRVAVSTRFPFTGFRATLHP-EIR
Mvan_3824|M.vanbaalenii_PYR-1 SGNAPGELHTPPPVRVSDYVSRAPGPLRIAMSTKFPFTVFRAGLHP-EIR
TH_4482|M.thermoresistible__bu SGNAPGDLHQPPPVRVSDYVGQAPGPLRIAVSTKFPFTLFRAKLHP-EIR
MMAR_3673|M.marinum_M SGNVEGDLHKPAPLTASDYVGIAPGPLKIAMSTRFPYTGFRAKLHP-EIL
MUL_3616|M.ulcerans_Agy99 SGNAEGDLHKPAPLTASDYVGIAPGPLKIAMSTRFPYTGFRAKLHP-EIL
Mb3409|M.bovis_AF2122/97 AG-SDIHDPSCSAEPVPDYAADLALTRIPRVGVDWS---QTTSFDE-DTT
Rv3375|M.tuberculosis_H37Rv AG-SDIHDPSCSAEPVPDYAADLALTRIPRVGVDWS---QTTSFDE-DTT
MAB_4643c|M.abscessus_ATCC_199 AG-FDDEDPLSLPSDGVDYLAALDQP-IESGRMAWSPDLGISKCDS-EIQ
MSMEG_2497|M.smegmatis_MC2_155 SR-FDGRDGTAMPTPTSSFLDGLDAG-VAGLRVAYSPNLGYVHNDP-EVD
MLBr_01596|M.leprae_Br4923 ------EAERIWHNPKLPPVGDVTQPGRQRLRIAVVTRSVQRECSP-ELR
MAV_0676|M.avium_104 IG-HHPDASYRVALPDKPFAQQITRPPGP-LTIGYSARSALNPRPDREAV
:
Mflv_2712|M.gilvum_PYR-GCK SALDGVADQLSQLGHTVLNADPDYTLGMSWNFLARS---TSGIPEWMDRL
Mvan_3824|M.vanbaalenii_PYR-1 TALQNVADQLTQLGHTIVAADPDYHLRMSWNFLSRS---TSGIPEWMDRL
TH_4482|M.thermoresistible__bu AAVQTVADQLEQLGHTVFPADPEYTVGMSWSFLSRS---TAGLLDWSRRL
MMAR_3673|M.marinum_M AATRRVGEQLQLLGHTVERGNPDYGLRLSWDFLARS---TAGLWEWAECL
MUL_3616|M.ulcerans_Agy99 ASTRRVGEQLQLLGHTVERGNPDYGLRLSWDFLARS---TAGLWEWAECL
Mb3409|M.bovis_AF2122/97 AMLADVVKTLDDIGWPVIDVKLPALAPMVAAFGKMR-AVETAIAHADTYP
Rv3375|M.tuberculosis_H37Rv AMLADVVKTLDDIGWPVIDVKLPALAPMVAAFGKMR-AVETAIAHADTYP
MAB_4643c|M.abscessus_ATCC_199 GICEEALIAFVEMGWTVEEDRPDWTDPAKTMWEGVWGPVYAGKLDAANWD
MSMEG_2497|M.smegmatis_MC2_155 AAVRAAVDVIAAAGAEVEEVDPGFADP-VEAFHVLWFSGEAHVLRGYG-D
MLBr_01596|M.leprae_Br4923 ELTLKSARLLEELGHRVERVAEPPVPPNFPDDFLLYWGLLAAMQVRTGRL
MAV_0676|M.avium_104 AAVENSAALLSALGHRVREVAPPYDDAALGRDFLTIWFAQLHQQVADIRR
: * :
Mflv_2712|M.gilvum_PYR-GCK GHRVTWDSRTRSNARMG----RLLSQHTLRKARAREAAAQQRIGWIFNLA
Mvan_3824|M.vanbaalenii_PYR-1 GRGVNWDSRTRSNARMG----WLLSQNVLRKARAGEAASQQRIGWIFNLA
TH_4482|M.thermoresistible__bu GDNVLWDKRTVANMRLG----RLLSESALRKARAREAAAQRRMSWIFNIA
MMAR_3673|M.marinum_M GDGVTLDPRTVTNMRAG----HALSQAILRNARHHEAAAQRRVGSIFDIV
MUL_3616|M.ulcerans_Agy99 GNGVTLDPRTVTNMRAG----HALSQAILRNARHHEAAAQRRVGSIFDIV
Mb3409|M.bovis_AF2122/97 ARADEYGPIMRAMIDAG----HRLAAVEYQTLTERRLEFTRSLRRVFHDV
Rv3375|M.tuberculosis_H37Rv ARADEYGPIMRAMIDAG----HRLAAVEYQTLTERRLEFTRSLRRVFHDV
MAB_4643c|M.abscessus_ATCC_199 DQAGHVDQELVQVIRDG----ARLTTVQVQRADAARGKMVDTLREFLRRF
MSMEG_2497|M.smegmatis_MC2_155 AVDDRVDPGLRRTAAIG----ATFSAADYLDATAVRMDLGLRMGRFHQTY
MLBr_01596|M.leprae_Br4923 AFGNTFDRTKLDSLTLGLDRHASRNMHRLPKAIMRLRRLRRRTADFFATY
MAV_0676|M.avium_104 RTGARDNDFEADTLAIS-ELGRSGGVLPVMSALENRNTYIQSLAAFHEGY
. . .
Mflv_2712|M.gilvum_PYR-GCK DVVVAPTTAQPPPEAHAFDGLGGLATDRMMIRA-----------------
Mvan_3824|M.vanbaalenii_PYR-1 DVVLAPTTALPPPEVRAFDDLGGLATDRMMIKA-----------------
TH_4482|M.thermoresistible__bu DVVLAPTTALPPPEVHAFDQLGGLATDRLMIKA-----------------
MMAR_3673|M.marinum_M DVVLAPTTAQPPPLARAFDDMGGLATDRAIIAA-----------------
MUL_3616|M.ulcerans_Agy99 DVVLAPTTAQPPPLARAFDDMGGLATDRAIIAA-----------------
Mb3409|M.bovis_AF2122/97 DILLMPSAGIASPTLETMR-GLGQDPELTARLA-----------------
Rv3375|M.tuberculosis_H37Rv DILLMPSAGIASPTLETMR-GLGQDPELTARLA-----------------
MAB_4643c|M.abscessus_ATCC_199 DFLVTPVTTAQAFGHDEFCPAVLAGAPLLERLMG----------------
MSMEG_2497|M.smegmatis_MC2_155 DLLLTPTLPLPAFPAGQDVPDGWHSPDWTSWTP-----------------
MLBr_01596|M.leprae_Br4923 DVLLTPTVADETPRIGYLTPTDYQQVMDRLMG------------------
MAV_0676|M.avium_104 DFFLTPTLATPPLVVGATSTSAALQRAARLVSRVRGGKLMRLSGILDDLI
*..: * .
Mflv_2712|M.gilvum_PYR-GCK ------CPVTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPADSEPLLIS
Mvan_3824|M.vanbaalenii_PYR-1 ------CPVTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEPLLVS
TH_4482|M.thermoresistible__bu ------CPVTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEPLLVS
MMAR_3673|M.marinum_M ------CPLTWPWNLLGWPSINVPAGFTVDGLPIGVQLMGPAESEGLLIS
MUL_3616|M.ulcerans_Agy99 ------CPLTWPWNLLGWPSINVPAGFTVDGLPIGVQLMGPAESEGLLIS
Mb3409|M.bovis_AF2122/97 -------MPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVR
Rv3375|M.tuberculosis_H37Rv -------MPTAPFNVSGNPAICLPAGTTARGTPLGVQFIGREFDEHLLVR
MAB_4643c|M.abscessus_ATCC_199 ------WVLTYPFNMTTNPAISIPVGFTRNGVPVGLQIVGRLRADADVLR
MSMEG_2497|M.smegmatis_MC2_155 --------YTYPFNLSQQPALTVPCGFTSAGLPIGLQVVGPRHSDSLVLR
MLBr_01596|M.leprae_Br4923 -----WVAFTPLQNVTGEPAISLPLAQSADGMPVGMMFTADFGQEAQLLE
MAV_0676|M.avium_104 RESLGWVPYTQLANLTGRPAISVPLHWTDDGLPLGVQFVGKLGADGDLLQ
* *: *:: :* : * *:*: . . : ::
Mflv_2712|M.gilvum_PYR-GCK LAAALEAINGWATLQPENWWAPQPPTEPDL----TAPDSVTGQVA
Mvan_3824|M.vanbaalenii_PYR-1 LAAALEAINGWAVHQPEDWWTPQPPPEPDLGSNVAAPASVEGQVA
TH_4482|M.thermoresistible__bu LAAALEGINGWAARQPRPWWLT-PPAAPXX----XASAAAS----
MMAR_3673|M.marinum_M LAAELEAVSGWATKQPKVWWNTGTSTPPIHS---VRPPRR-----
MUL_3616|M.ulcerans_Agy99 LAAELEAVSGWATKQPKVWWNTGTSTPPIHS---VRPPRR-----
Mb3409|M.bovis_AF2122/97 AGHAFQQVTGYHRRRPPV---------------------------
Rv3375|M.tuberculosis_H37Rv AGHAFQQVTGYHRRRPPV---------------------------
MAB_4643c|M.abscessus_ATCC_199 LAAAFEAVRPWAQRRPSDAVLNGENP-------------------
MSMEG_2497|M.smegmatis_MC2_155 VGQAYQSATDWHLKTPKVLTGEGH---------------------
MLBr_01596|M.leprae_Br4923 LAFELEEARPWARIQIGD---------------------------
MAV_0676|M.avium_104 LAAQLEQARPWAQRHPAPVTPPN----------------------
. : :