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M. marinum M MMAR_0284 (plcB_3)

annotation: membrane-associated phospholipase C 2 PlcB_3
coordinates: 325297 - 327039
length: 580

VAWGVIAAAVQWGNGNGPLGIEFKFLRTNSKNKRDTRVGAPFQLAEYVYSEPNAQGGYGLSNGSVDGMSR
RQFMAKLAAASSAGALMSLAGPVIEKAYGAGPCSGHLTDIEHIVLLMQENRSFDHYFGTLSGVRGFDDTT
DPAIFDQKGWDPRTQSIDPAGITSPFRFDTTRGPLLNGECVNDPVEGWVAMHTAWNNGANDTWLPSQYSQ
IRQGNIPACMGYYTRADLPIHYMLADTFTICDDYYCSMLTGTAPNRLYWLSAWIDPDGTNGGPLLNEPNF
LPLQPFSWRIMPENLEDAGISWKVYQNKFFGHYINSPISDNGLVQAFKQTADPRSNLARFGIAPTYPQDF
VLDVKANRLPKVSWVVPNIIQSEHPALPVNVGAVAIVNILRTLLSNPAVWEKTALIISYDECGSFFDHVT
PPTAPPGTPGEYVTVPDIDAVSGSGGIRGPIGLGYRVPCIVISPYSRGPLVVSDTFDHTSQLRLIETRFG
VPVPNLTPWRRSVTEDMTSAFNFAVPPNPSRPKLDPPLLNALPRLPQCVPNAVLGSLGGAAVANPYRVPY
PQVPPTQETTPARGTPSGPC

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. marinum M MMAR_0284 plcB_3 - 100% (580) membrane-associated phospholipase C 2 PlcB_3
M. marinum M MMAR_3657 plcB_4 0.0 72.94% (521) membrane-associated phospholipase C 2 PlcB_4
M. marinum M MMAR_3823 plcB_1 0.0 71.46% (522) membrane-associated phospholipase C 2 PlcB_1

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb1784c plcD 0.0 69.29% (521) phospholipase C 4 PLCD
M. gilvum PYR-GCK - - - - -
M. tuberculosis H37Rv Rv2350c plcB 0.0 73.98% (515) membrane-associated phospholipase C
M. leprae Br4923 - - - - -
M. abscessus ATCC 19977 MAB_0555 - 8e-90 37.45% (518) non-hemolytic phospholipase C
M. avium 104 - - - - -
M. smegmatis MC2 155 - - - - -
M. thermoresistible (build 8) - - - - -
M. ulcerans Agy99 MUL_2799 plcB 0.0 70.74% (516) membrane-associated phospholipase C 2 PlcB
M. vanbaalenii PYR-1 - - - - -

CLUSTAL 2.0.9 multiple sequence alignment


Rv2350c|M.tuberculosis_H37Rv        --------------------------------------------------
MUL_2799|M.ulcerans_Agy99           --------------------------------------------------
MMAR_0284|M.marinum_M               MAWGVIAAAVQWGNGNGPLGIEFKFLRTNSKNKRDTRVGAPFQLAEYVYS
Mb1784c|M.bovis_AF2122/97           --------------------------------------------------
MAB_0555|M.abscessus_ATCC_1997      --------------------------------------------------
                                                                                      

Rv2350c|M.tuberculosis_H37Rv        -----------------MTRRQFFAKAAAATTAGAFMSLAGPIIEKAYG-
MUL_2799|M.ulcerans_Agy99           -----------------MSRREFMAKLAAASSAGAMMSLAGPVIEKAYG-
MMAR_0284|M.marinum_M               EPNAQGGYGLSNGSVDGMSRRQFMAKLAAASSAGALMSLAGPVIEKAYG-
Mb1784c|M.bovis_AF2122/97           ---------MSQSHIGGVSRREFLAKVAAGG-AGALMSFAGPVIEKAYG-
MAB_0555|M.abscessus_ATCC_1997      --------------MSPLTRRRLLTSAAGTAAFSAAVSLMPANLQRVLAQ
                                                     ::**.:::. *.    .* :*:  . :::. . 

Rv2350c|M.tuberculosis_H37Rv        AGPCPGHLTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDTTPPVVFAQSG
MUL_2799|M.ulcerans_Agy99           AGPCSGHLTDIEHFVFMLQENRSFDHYFGSLSGINGFD--TPSSAFQQKG
MMAR_0284|M.marinum_M               AGPCSGHLTDIEHIVLLMQENRSFDHYFGTLSGVRGFDDTTDPAIFDQKG
Mb1784c|M.bovis_AF2122/97           AGPCSGHLTDIEHFVFFMQENRSFDHYFGTLSGTDGFN--TVSPLFQQKG
MAB_0555|M.abscessus_ATCC_1997      PAPRSGSLRDVEHVVVLMMENRSFDHYFGTLSGVRGFDDPTAVTLSTGKS
                                    ..* .* * *:**.*.:: **********:**.  **:  *       ..

Rv2350c|M.tuberculosis_H37Rv        WNPMTQAVDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGA
MUL_2799|M.ulcerans_Agy99           WNPQTQSIDPAGVTIPYRFDTTRGPLLNGACANDPGHQWIDMHRAFNNGA
MMAR_0284|M.marinum_M               WDPRTQSIDPAGITSPFRFDTTRGPLLNGECVNDPVEGWVAMHTAWNNGA
Mb1784c|M.bovis_AF2122/97           WNPMTQALDATGVTMPYRFDTTRGPFLDGACVNDPDHSWVAMHESWNGGV
MAB_0555|M.abscessus_ATCC_1997      VFHQPDPQNPDGYLLPFHLDTAVNSVQK---IPTTSHAWSVQHDSWNHGK
                                        .:. :. *   *:::**: ...        . . *   * ::* * 

Rv2350c|M.tuberculosis_H37Rv        NDNWLPAQVPFSPLQGNVPVTMGFYTRRDLPIHYLLADTFTVCDGYFCSL
MUL_2799|M.ulcerans_Agy99           NDNWLGAQAQAQTLQGNVPVTMGYYTRQDQPIHYLLADTFTICDGYHCSL
MMAR_0284|M.marinum_M               NDTWLPSQYS-QIRQGNIPACMGYYTRADLPIHYMLADTFTICDDYYCSM
Mb1784c|M.bovis_AF2122/97           NDNWLPAQAK-TRSAAHTPTVMGYYTRQDIPIHYLLADAFTVCDRYFCSV
MAB_0555|M.abscessus_ATCC_1997      MDNWMAAHRK--ADGEHYPYVMGYYERADIPFHFALAEAFTVCDNFHSSV
                                     *.*: ::        : *  **:* * * *:*: **::**:** :..*:

Rv2350c|M.tuberculosis_H37Rv        LGGTTPNRLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDA
MUL_2799|M.ulcerans_Agy99           IGGTSPNRLYWMSAWVDPNGEQGGPLLVDPNIQPQGRFSWRTMPDNLSDA
MMAR_0284|M.marinum_M               LTGTAPNRLYWLSAWIDPDGTNGGPLLNEPNFLPLQPFSWRIMPENLEDA
Mb1784c|M.bovis_AF2122/97           LGPTLPNRLYWLSATIDPDGQNGGPELQSPTFQPVRRFGWRIMPQNLSDA
MAB_0555|M.abscessus_ATCC_1997      MGPTWPNRMMWMTGTIDPDAAHGGPIIRNTPVS--GGYGWTTYAERLEAA
                                    :  * ***: *::. :**:. .*** : .. .     :.*   .:.*. *

Rv2350c|M.tuberculosis_H37Rv        GVSWKVYQN-KLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYP
MUL_2799|M.ulcerans_Agy99           GISWKVYQN-KLLGALNNTVIGYDGMLNDFVQARDPRSNMARYGIAPTYP
MMAR_0284|M.marinum_M               GISWKVYQN-KFFGHYINSPISDNGLVQAFKQTADPRSNLARFGIAPTYP
Mb1784c|M.bovis_AF2122/97           GVSWKVYRN-KTLGPIS-SVLTYGSLVTSFKQSADPRSDLVRFGVAPSYP
MAB_0555|M.abscessus_ATCC_1997      GVSWRVYAEEDDYGGCN--VLK---LFSRFQRSR-PGEPLYDKGIAPVRA
                                    *:**:** : .  *      :    :.  * ::  * . :   *::*  .

Rv2350c|M.tuberculosis_H37Rv        LDFAADVRNNRLPKVSWVLPGFLLSEHPAFPVN--VGAVAIVDALRILLS
MUL_2799|M.ulcerans_Agy99           LDFVADVASNRLPQVSWVLPGFQVSEHPGVPVAPAVGGTAIVNILRILLS
MMAR_0284|M.marinum_M               QDFVLDVKANRLPKVSWVVPNIIQSEHPALPVN--VGAVAIVNILRTLLS
Mb1784c|M.bovis_AF2122/97           ASFAADVLANRLPRVSWVIPNVLESEHPAVPAA--AGAFAIVNILRILLA
MAB_0555|M.abscessus_ATCC_1997      TQFEDDARAGRLPAVSWLIMTSTASEHPDYRPA--DGAAYIASKIDAIAA
                                     .*  *.  .*** ***::     ****        *.  *.. :  : :

Rv2350c|M.tuberculosis_H37Rv        NPAVWEKTALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSG
MUL_2799|M.ulcerans_Agy99           NPAVWEKTALIVSFDENGGFFDHVTPTTAPEGTPGEWITVPDINAVTGSG
MMAR_0284|M.marinum_M               NPAVWEKTALIISYDECGSFFDHVTPPTAPPGTPGEYVTVPDIDAVSGSG
Mb1784c|M.bovis_AF2122/97           NPAVWEKTALIVSYDENGGFFDHVVPATAPAGTPGEYVTVPDIDQVPGSG
MAB_0555|M.abscessus_ATCC_1997      NPEVWAKTVFILNYDENDGLFDHVVPPTPPPGTPGEWVKG----------
                                    ** ** **.:*:.:** ..:****.*.*.* *****::.           

Rv2350c|M.tuberculosis_H37Rv        GIRGPIGLGFRVPCLVISPYSRGPLMVHDTFDHTSTLKLIRARFGVPVPN
MUL_2799|M.ulcerans_Agy99           GIRGPIGLGYRVPCFVISPYSRGPLMVHDIFDHTSQLRLLEQRFGVPVPN
MMAR_0284|M.marinum_M               GIRGPIGLGYRVPCIVISPYSRGPLVVSDTFDHTSQLRLIETRFGVPVPN
Mb1784c|M.bovis_AF2122/97           GIRGPIGLGFRVPCFVISPYSRGPQMVHDTFDHTSQLRLLETRFGVPVPN
MAB_0555|M.abscessus_ATCC_1997      ---VPIGGGFRVPAIVISPWSVGGWVAGESFDHTSVLRFLEEFTGVREPN
                                        *** *:***.:****:* *  :. : ***** *:::.   **  **

Rv2350c|M.tuberculosis_H37Rv        LTAWRDATVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLG
MUL_2799|M.ulcerans_Agy99           LTTWRRGVTGDMTSTFNFAVPPNASPPNLDHPALKAVPQQVQCVPDTVT-
MMAR_0284|M.marinum_M               LTPWRRSVTEDMTSAFNFAVPPNPSRPKLDPPLLNALPRLPQCVPNAVLG
Mb1784c|M.bovis_AF2122/97           LTAWRRSVTGDMTSTFNFAVPPNSSWPNLDYPGLHALSTVPQCVPNAALG
MAB_0555|M.abscessus_ATCC_1997      ISGWRRKTFGNLLSAFGFDSPVRPAPELPDTAAVVAAADRTDTRP-----
                                    :: **  .  :: *:*.*  * ..:    * . : * .   :  *     

Rv2350c|M.tuberculosis_H37Rv        TVTKTAIP--YRVPFPQSMPTQE-TAPTRGIPSGLC
MUL_2799|M.ulcerans_Agy99           VFTKTVPP--YRVPFPQVMPSQE-TSPTRGIPSGPC
MMAR_0284|M.marinum_M               SLGGAAVANPYRVPYPQVPPTQE-TTPARGTPSGPC
Mb1784c|M.bovis_AF2122/97           TINR-GIP--YRVPDPQIMPTQE-TTPTRGIPSGPC
MAB_0555|M.abscessus_ATCC_1997      ---PPVIP-----PAPQTSPVQEPGSRPRRYPRR--
                                           .     * **  * **  : .*  *

species	id	gene	e-value	identity (len)	annotation
M. marinum M	MMAR_0284	plcB_3	-	100% (580)	membrane-associated phospholipase C 2 PlcB_3
M. marinum M	MMAR_3657	plcB_4	0.0	72.94% (521)	membrane-associated phospholipase C 2 PlcB_4
M. marinum M	MMAR_3823	plcB_1	0.0	71.46% (522)	membrane-associated phospholipase C 2 PlcB_1