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M. ulcerans Agy99 MUL_2799 (plcB)

annotation: membrane-associated phospholipase C 2 PlcB
coordinates: 3125957 - 3127492
length: 511

SRREFMAKLAAASSAGAMMSLAGPVIEKAYGAGPCSGHLTDIEHFVFMLQENRSFDHYFGSLSGINGFDT
PSSAFQQKGWNPQTQSIDPAGVTIPYRFDTTRGPLLNGACANDPGHQWIDMHRAFNNGANDNWLGAQAQA
QTLQGNVPVTMGYYTRQDQPIHYLLADTFTICDGYHCSLIGGTSPNRLYWMSAWVDPNGEQGGPLLVDPN
IQPQGRFSWRTMPDNLSDAGISWKVYQNKLLGALNNTVIGYDGMLNDFVQARDPRSNMARYGIAPTYPLD
FVADVASNRLPQVSWVLPGFQVSEHPGVPVAPAVGGTAIVNILRILLSNPAVWEKTALIVSFDENGGFFD
HVTPTTAPEGTPGEWITVPDINAVTGSGGIRGPIGLGYRVPCFVISPYSRGPLMVHDIFDHTSQLRLLEQ
RFGVPVPNLTTWRRGVTGDMTSTFNFAVPPNASPPNLDHPALKAVPQQVQCVPDTVTVFTKTVPPYRVPF
PQVMPSQETSPTRGIPSGPC*

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. ulcerans Agy99 MUL_2799 plcB - 100% (511) membrane-associated phospholipase C 2 PlcB

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb1784c plcD 0.0 69.47% (511) phospholipase C 4 PLCD
M. gilvum PYR-GCK - - - - -
M. tuberculosis H37Rv Rv2350c plcB 0.0 73.54% (514) membrane-associated phospholipase C
M. leprae Br4923 - - - - -
M. abscessus ATCC 19977 MAB_0555 - 2e-87 36.78% (503) non-hemolytic phospholipase C
M. marinum M MMAR_3566 plcB 0.0 99.61% (511) membrane-associated phospholipase C 2 PlcB
M. avium 104 - - - - -
M. smegmatis MC2 155 - - - - -
M. thermoresistible (build 8) - - - - -
M. vanbaalenii PYR-1 - - - - -

CLUSTAL 2.0.9 multiple sequence alignment


MUL_2799|M.ulcerans_Agy99           ----------MSRREFMAKLAAASSAGAMMSLAGPVIEKAYG-AGPCSGH
MMAR_3566|M.marinum_M               MLVSKHPVDGMSRREFMAKLAAASSAGAMMSLAGPVIEKAYG-AGPCSGH
Rv2350c|M.tuberculosis_H37Rv        ----------MTRRQFFAKAAAATTAGAFMSLAGPIIEKAYG-AGPCPGH
Mb1784c|M.bovis_AF2122/97           --MSQSHIGGVSRREFLAKVAAGG-AGALMSFAGPVIEKAYG-AGPCSGH
MAB_0555|M.abscessus_ATCC_1997      -------MSPLTRRRLLTSAAGTAAFSAAVSLMPANLQRVLAQPAPRSGS
                                              ::**.:::. *.    .* :*:  . :::. . ..* .* 

MUL_2799|M.ulcerans_Agy99           LTDIEHFVFMLQENRSFDHYFGSLSGINGFD--TPSSAFQQKGWNPQTQS
MMAR_3566|M.marinum_M               LTDIEHFVFMLQENRSFDHYFGSLSGINGFD--TPSSAFQQKGWNPQTQS
Rv2350c|M.tuberculosis_H37Rv        LTDIEHIVLLMQENRSFDHYFGTLSDTRGFDDTTPPVVFAQSGWNPMTQA
Mb1784c|M.bovis_AF2122/97           LTDIEHFVFFMQENRSFDHYFGTLSGTDGFN--TVSPLFQQKGWNPMTQA
MAB_0555|M.abscessus_ATCC_1997      LRDVEHVVVLMMENRSFDHYFGTLSGVRGFDDPTAVTLSTGKSVFHQPDP
                                    * *:**.*.:: **********:**.  **:  *       ..    .:.

MUL_2799|M.ulcerans_Agy99           IDPAGVTIPYRFDTTRGPLLNGACANDPGHQWIDMHRAFNNGANDNWLGA
MMAR_3566|M.marinum_M               IDPAGVTIPYRFDTTRGPLLNGACVNDPGHQWIDMHRAFNNGANDNWLGA
Rv2350c|M.tuberculosis_H37Rv        VDPAGVTLPYRFDTTRGPLVAGECVNDPDHSWIGMHNSWNGGANDNWLPA
Mb1784c|M.bovis_AF2122/97           LDATGVTMPYRFDTTRGPFLDGACVNDPDHSWVAMHESWNGGVNDNWLPA
MAB_0555|M.abscessus_ATCC_1997      QNPDGYLLPFHLDTAVNSVQK---IPTTSHAWSVQHDSWNHGKMDNWMAA
                                     :. *  :*:::**: ...        ..* *   * ::* *  ***: *

MUL_2799|M.ulcerans_Agy99           QAQAQTLQGNVPVTMGYYTRQDQPIHYLLADTFTICDGYHCSLIGGTSPN
MMAR_3566|M.marinum_M               QAQAQTLQGNVPVTMGYYTRQDQPIHYLLADTFTICDGYHCSLIGGTSPN
Rv2350c|M.tuberculosis_H37Rv        QVPFSPLQGNVPVTMGFYTRRDLPIHYLLADTFTVCDGYFCSLLGGTTPN
Mb1784c|M.bovis_AF2122/97           QAKTRSAA-HTPTVMGYYTRQDIPIHYLLADAFTVCDRYFCSVLGPTLPN
MAB_0555|M.abscessus_ATCC_1997      HRKADGEH--YPYVMGYYERADIPFHFALAEAFTVCDNFHSSVMGPTWPN
                                    :          * .**:* * * *:*: **::**:** :..*::* * **

MUL_2799|M.ulcerans_Agy99           RLYWMSAWVDPNGEQGGPLLVDPNIQPQGRFSWRTMPDNLSDAGISWKVY
MMAR_3566|M.marinum_M               RLYWMSAWIDPNGEQGGPLLVDPNIQPQGRFSWRTMPDNLSDAGISWKVY
Rv2350c|M.tuberculosis_H37Rv        RLYWMSAWIDPDGTDGGPVLIEPNIQPLQHYSWRIMPENLEDAGVSWKVY
Mb1784c|M.bovis_AF2122/97           RLYWLSATIDPDGQNGGPELQSPTFQPVRRFGWRIMPQNLSDAGVSWKVY
MAB_0555|M.abscessus_ATCC_1997      RMMWMTGTIDPDAAHGGPIIRNTPVS--GGYGWTTYAERLEAAGVSWRVY
                                    *: *::. :**:. .*** : .. ..    :.*   .:.*. **:**:**

MUL_2799|M.ulcerans_Agy99           QN-KLLGALNNTVIGYDGMLNDFVQARDPRSNMARYGIAPTYPLDFVADV
MMAR_3566|M.marinum_M               QN-KLLGALNNTVIGYDGMLNDFVQARDPRSNMARYGIAPTYPLDFVADV
Rv2350c|M.tuberculosis_H37Rv        QN-KLLGALNNTVVGYNGLVNDFKQAADPRSNLARFGISPTYPLDFAADV
Mb1784c|M.bovis_AF2122/97           RN-KTLGPIS-SVLTYGSLVTSFKQSADPRSDLVRFGVAPSYPASFAADV
MAB_0555|M.abscessus_ATCC_1997      AEEDDYGGCN-----VLKLFSRFQRSR-PGEPLYDKGIAPVRATQFEDDA
                                     : .  *  .        :.. * ::  * . :   *::*  . .*  *.

MUL_2799|M.ulcerans_Agy99           ASNRLPQVSWVLPGFQVSEHPGVPVAPAVGGTAIVNILRILLSNPAVWEK
MMAR_3566|M.marinum_M               ASNRLPQVSWVLPGFQVSEHPGVPVAPAVGGTAIVNILRILLSNPAVWEK
Rv2350c|M.tuberculosis_H37Rv        RNNRLPKVSWVLPGFLLSEHPAFPVN--VGAVAIVDALRILLSNPAVWEK
Mb1784c|M.bovis_AF2122/97           LANRLPRVSWVIPNVLESEHPAVPAA--AGAFAIVNILRILLANPAVWEK
MAB_0555|M.abscessus_ATCC_1997      RAGRLPAVSWLIMTSTASEHPDYRPA--DGAAYIASKIDAIAANPEVWAK
                                      .*** ***::     ****        *.  *.. :  : :** ** *

MUL_2799|M.ulcerans_Agy99           TALIVSFDENGGFFDHVTPTTAPEGTPGEWITVPDINAVTGSGGIRGPIG
MMAR_3566|M.marinum_M               TALIVSFDENGGFFDHVTPTTAPEGTPGEWITVPDINAVTGSGGIRGPIG
Rv2350c|M.tuberculosis_H37Rv        TALIVNYDENGGFFDHVVPPTPPPGTPGEFVTVPDIDSVPGSGGIRGPIG
Mb1784c|M.bovis_AF2122/97           TALIVSYDENGGFFDHVVPATAPAGTPGEYVTVPDIDQVPGSGGIRGPIG
MAB_0555|M.abscessus_ATCC_1997      TVFILNYDENDGLFDHVVPPTPPPGTPGEWVKG-------------VPIG
                                    *.:*:.:***.*:****.*.*.* *****::.               ***

MUL_2799|M.ulcerans_Agy99           LGYRVPCFVISPYSRGPLMVHDIFDHTSQLRLLEQRFGVPVPNLTTWRRG
MMAR_3566|M.marinum_M               LGYRVPCFVISPYSRGPLMVHDIFDHTSQLRLLEQRFGVPVPNLTTWRRG
Rv2350c|M.tuberculosis_H37Rv        LGFRVPCLVISPYSRGPLMVHDTFDHTSTLKLIRARFGVPVPNLTAWRDA
Mb1784c|M.bovis_AF2122/97           LGFRVPCFVISPYSRGPQMVHDTFDHTSQLRLLETRFGVPVPNLTAWRRS
MAB_0555|M.abscessus_ATCC_1997      GGFRVPAIVISPWSVGGWVAGESFDHTSVLRFLEEFTGVREPNISGWRRK
                                     *:***.:****:* *  :. : ***** *:::.   **  **:: **  

MUL_2799|M.ulcerans_Agy99           VTGDMTSTFNFAVPPNASPPNLDHPALKAVPQQVQCVPDTVT-VFTKTVP
MMAR_3566|M.marinum_M               VTGDMTSTFNFAVPPNASPPNLDHPALKAVPQQVQCVPDTVT-VFTKTVP
Rv2350c|M.tuberculosis_H37Rv        TVGDMTSTFNFAAPPNPSKPNLDHPRLNALPKLPQCVPNAVLGTVTKTAI
Mb1784c|M.bovis_AF2122/97           VTGDMTSTFNFAVPPNSSWPNLDYPGLHALSTVPQCVPNAALGTINR-GI
MAB_0555|M.abscessus_ATCC_1997      TFGNLLSAFGFDSPVRPAPELPDTAAVVAAADRTDTRP-----------P
                                    . *:: *:*.*  * ..:    * . : * .   :  *            

MUL_2799|M.ulcerans_Agy99           PYRVPFPQVMPSQE-TSPTRGIPSGPC
MMAR_3566|M.marinum_M               PYRVPFPQVMPSQE-TSPTRGIPSGPC
Rv2350c|M.tuberculosis_H37Rv        PYRVPFPQSMPTQE-TAPTRGIPSGLC
Mb1784c|M.bovis_AF2122/97           PYRVPDPQIMPTQE-TTPTRGIPSGPC
MAB_0555|M.abscessus_ATCC_1997      PVIPPAPQTSPVQEPGSRPRRYPRR--
                                    *   * **  * **  : .*  *

species	id	gene	e-value	identity (len)	annotation
M. ulcerans Agy99	MUL_2799	plcB	-	100% (511)	membrane-associated phospholipase C 2 PlcB