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SSVVGQTFEHFTDVLVIGSGGGGMTAALAADASGLDTLVVEKSPRFGGSTALSGGGIWVPGAPSQRREGY VPDPDEVFTYLKEITGGLVSDARLRKYVDAAPEMMEFLEKLSPWLEFVWKPGYADYYPELPGGSALGSTI NVPEIDLRVLGDEEQHLLAPLALAPRGIWFAPKDLRLFYQVRQSWRGKAVLLKLIWRMFRARVFGDRMAA IGQSLSARLRLAMKQQNIPLWLNAPMTELITDTDGPEGRVVGAVIERDGRPIRVRARGGVILASGGFDHD MQWRRQHLPELNRVDELAGCGKDWSFGNPAATGDGIRAGEKVGAATDLLDEAWWFPAICWPDGRLQFMLN ERMMPSQFVVNGDGERFINEAAPYMDFAHAMIEGQRSGVTHIPCWLITDIRSFHRYVVAGHLPIPKVPFA PVPTGWKVPAAWLESGVVKTGSTWEELADQIGVPGTRLRGTAERFNALAAKGHDDDFNRGDSAYDNYYGD PTLPNPNLHPLGAPPYFAFQIILGDLGTSGGLRTDEHARVLRGDDSVIGGLYAVGNASAAVMGRSYAGAG ATIGPAMTFGYVAARHIADSAGGTTDRTTTSTESPGSSRR*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_4243 | - | - | 100% (601) | 3-ketosteroid-delta-1-dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_5237 | - | e-122 | 42.63% (577) | 3-ketosteroid-delta-1-dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_2868 | - | e-111 | 40.91% (572) | 3-ketosteroid-delta-1-dehydrogenase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3567 | - | 1e-126 | 42.86% (581) | 3-ketosteroid-delta-1-dehydrogenase |
| M. gilvum PYR-GCK | Mflv_2403 | - | 0.0 | 88.52% (575) | 3-ketosteroid-delta-1-dehydrogenase |
| M. tuberculosis H37Rv | Rv3537 | - | 1e-126 | 42.86% (581) | 3-ketosteroid-delta-1-dehydrogenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0622c | - | 1e-124 | 42.53% (576) | 3-ketosteroid-delta-1-dehydrogenase |
| M. marinum M | MMAR_4775 | - | 0.0 | 74.04% (570) | dehydrogenase |
| M. avium 104 | MAV_0880 | - | 0.0 | 74.96% (571) | 3-ketosteroid-delta-1-dehydrogenase |
| M. smegmatis MC2 155 | MSMEG_4864 | - | 0.0 | 83.89% (571) | 3-ketosteroid-delta-1-dehydrogenase |
| M. thermoresistible (build 8) | TH_4337 | - | 0.0 | 80.73% (602) | PUTATIVE fumarate reductase/succinate dehydrogenase |
| M. ulcerans Agy99 | MUL_0346 | - | 0.0 | 74.04% (570) | 3-ketosteroid-delta-1-dehydrogenase |
CLUSTAL 2.0.9 multiple sequence alignment
Mvan_4243|M.vanbaalenii_PYR-1 MSS--VVGQTFEHFTDVLVIGSGGGGMTAALAADASGLDTLVVEKSPRFG
Mflv_2403|M.gilvum_PYR-GCK MTERRSTVQSFDHVTDVLVVGSGGGGMTAALAADAAGLDTLVVEKSPRFG
MSMEG_4864|M.smegmatis_MC2_155 MTSTNSPTDGFDHTVDLLVIGSGGGGMTAALAGDASGLDTLVVEKSPQFG
TH_4337|M.thermoresistible__bu -----MTVDTHDQTVDVLVVGSGGGGMTAALTADAAGLDTLLVEKSPQFG
MMAR_4775|M.marinum_M --MTVGPWGDVDRMVDVLVVGSGGGGMTAALTAASSGLDTLVIEKSSCFG
MUL_0346|M.ulcerans_Agy99 --MTVGPWGDVDRMVDVLVVGSGGGGMTAALTAASSGLDTLVIEKSSCFG
MAV_0880|M.avium_104 -------MTDFDHLVDVLIVGSGGGGMTAALTAQAAGLDALIVEKSSHFG
Mb3567|M.bovis_AF2122/97 ---------MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYG
Rv3537|M.tuberculosis_H37Rv ---------MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYG
MAB_0622c|M.abscessus_ATCC_199 ---------MTEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFG
: *::::***..**.***.. **.::::**:. :*
Mvan_4243|M.vanbaalenii_PYR-1 GSTALSGGGIWVPGAPSQRREGYVPDPDEVFTYLKEITGGLVSDARLRKY
Mflv_2403|M.gilvum_PYR-GCK GSTALSGGGIWVPGAPSQRREGHVVDPEQVFTYLKQITGGLVSDARLRKY
MSMEG_4864|M.smegmatis_MC2_155 GSTALSGGGIWVPGAPAQRREGHVPSPDGVFEYLKQITGGLVSDARLRQY
TH_4337|M.thermoresistible__bu GSTALSGGGIWVPGAPAQRREGYSPDPDDVFEYLRQITGGLVSDARLRTY
MMAR_4775|M.marinum_M GSTALSGGGIWVPGAPAQRRAGYSPNPDDVVGYLRRITDGLVSEARIRQY
MUL_0346|M.ulcerans_Agy99 GSTALSGGGIWVPGDPAQRRAGYSPNPDDVVGYLRRITNGLVSEARIRQY
MAV_0880|M.avium_104 GSTALSGGGIWVPGAPAQRREGYSPSPEGVVEYLKNITDGLVSEARIRQY
Mb3567|M.bovis_AF2122/97 GSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVGEIVEPERIDAY
Rv3537|M.tuberculosis_H37Rv GSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVGEIVEPERIDAY
MAB_0622c|M.abscessus_ATCC_199 GSTARSGGGVWIPNNEVLKKARQTDTPEAAREYLYSIIGDVVPREKIDTY
**** ****:*:*. :: *: . ** * . :* :: *
Mvan_4243|M.vanbaalenii_PYR-1 VDAAPEMMEFLEKLSPWLEFVWKPGYADYYPELPGGSALGSTINVPEIDL
Mflv_2403|M.gilvum_PYR-GCK VEAAPEMMEFLENLSPWLEFVWKPGYADYYPELPGGSALGSTINVPEIDL
MSMEG_4864|M.smegmatis_MC2_155 VDSAPEMMDFLERTSNWMEFVWKPGYADYYPELPGGSALGSTINVPAIDL
TH_4337|M.thermoresistible__bu VDEAPKMMEFLEQLSPWCEFVWKPGYADYYPELPGGSPLGSTINVPAIDL
MMAR_4775|M.marinum_M VYSAPQMLEFLEGLSAWLEFVWKPGYADYYPELPGGSVLGSTINVPPIDL
MUL_0346|M.ulcerans_Agy99 VYSAPQMLEFLEGLSAWLEFVWKPGYADYYPELPGGSVLGSTINVPPIDL
MAV_0880|M.avium_104 VETAPQMLAFLENLSGWFEFVWKPGYADYYPELPGGSELGSTINVPPIDL
Mb3567|M.bovis_AF2122/97 LDRGPEMLSFVLKHTP-LKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNA
Rv3537|M.tuberculosis_H37Rv LDRGPEMLSFVLKHTP-LKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNA
MAB_0622c|M.abscessus_ATCC_199 LDRGPEMLSFVLANSP-LKLDWVPNYSDYYPEAPGGRAAGRSVEPKPFDA
: .*:*: *: : :: * *.*:***** *** * ::: ::
Mvan_4243|M.vanbaalenii_PYR-1 RVLGDEEQHLLAPLALAPRGIWFAPKDLRLFYQVRQSWRGKAVLLKLIWR
Mflv_2403|M.gilvum_PYR-GCK RTLGDEEQNLLTPLALAPKGIWFAPKDLRLFYQVRQSWKGKAVLVKLIWR
MSMEG_4864|M.smegmatis_MC2_155 RKLGDEERNLLTPLALAPKGIWFAPKDLRLFYQIRQNWRGKAVLLKLIWR
TH_4337|M.thermoresistible__bu RRLGDEEARLLQPLALAPRGIWFAPKDLRLFYQVRQNWRGKAVLVKLIWR
MMAR_4775|M.marinum_M RKLGDDQPRLLTPLALAPRGIWLGPKELRSFYRIRQSWAGKAVLLKLVTR
MUL_0346|M.ulcerans_Agy99 RKLGDDQPRLLTPLALAPRGIWLGPKELRSFYRIRQSWAGKAVLLKLVTR
MAV_0880|M.avium_104 RKLGPDEHKLLQPLALAPKGIWLGPKELRTFYRIRQSWAGKAVLLKLIAR
Mb3567|M.bovis_AF2122/97 RKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRGVLRSMKVGAR
Rv3537|M.tuberculosis_H37Rv RKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRGVLRSMKVGAR
MAB_0622c|M.abscessus_ATCC_199 KKLGDELPNLEPPYGKVPLNVVVRQQDYVRLNQLKRHPRGVLRSLRVGVR
: ** : * . . .* .: . :: : :::: * ::: *
Mvan_4243|M.vanbaalenii_PYR-1 MFRARVFGDRMAAIGQSLSARLRLAMKQQNIPLWLNAPMTELITDTDGPE
Mflv_2403|M.gilvum_PYR-GCK MFRARVFGDRMAAIGQSLSARLRLAMKQQDIPLWLNSPMTELITDHDGPD
MSMEG_4864|M.smegmatis_MC2_155 MFRARVFGDRMAAIGQSLAARLRLALKEQGIPLWLNSPMTELITADDG--
TH_4337|M.thermoresistible__bu MFRARVFGDRMAAIGQSLAARLRLAMRQQGVPLWLNAPMTELITDADG--
MMAR_4775|M.marinum_M MIRARVFGEQIAAIGQSLAARLRLAMKELGVALWLDAPLVELLTDAGG--
MUL_0346|M.ulcerans_Agy99 MIRARVFGEQIAAIGQSLAARLRLAMKELGVALWLDAPLVELLTDAGG--
MAV_0880|M.avium_104 MVRARVFGERMAAIGQSLAARMRLALRDRGVPLWLDSPMVRLLTDPQG--
Mb3567|M.bovis_AF2122/97 TMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
Rv3537|M.tuberculosis_H37Rv TMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
MAB_0622c|M.abscessus_ATCC_199 TFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVR
. *: *..:..:*::* . :*:.:: .:.: *::.:. *
Mvan_4243|M.vanbaalenii_PYR-1 GRVVGAVIERD-GRPIRVRARGGVILASGGFDHDMQWRRQHLPELNRVDE
Mflv_2403|M.gilvum_PYR-GCK ARVIGAVVERD-GRTLRVRADRGVVLASGGFDHDMQWRLQHLPELARVRE
MSMEG_4864|M.smegmatis_MC2_155 -AVIGAVVERD-GTPMRIGARRGVILASGGFDHDMAWRRQYLPILE----
TH_4337|M.thermoresistible__bu -AVVGAVVEKD-GRAVRIRARHGVILAAGGFDHDMDRRREHLPLLE----
MMAR_4775|M.marinum_M -AVTGAVVQTE-GGRERIGVRGGVILASGGFDHDLAWRKEHQPLVE----
MUL_0346|M.ulcerans_Agy99 -AVTGAVVQTE-GGRERIGVRGGVILASGGFDHDLAWRKEHQPLVE----
MAV_0880|M.avium_104 -AVSGAVVERE-GKPQRIGARFGVILASGGFDHDLAWRKELLPEVE----
Mb3567|M.bovis_AF2122/97 GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPIT---
Rv3537|M.tuberculosis_H37Rv GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPIT---
MAB_0622c|M.abscessus_ATCC_199 GVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPIT---
: . : **:**.***:*: * :
Mvan_4243|M.vanbaalenii_PYR-1 LAGCGKDWSFGNPAATGDGIRAGEKVGAATDLLDEAWWFPAICWPDGRLQ
Mflv_2403|M.gilvum_PYR-GCK MAPEGKDWSFGNPAAMGDGIRAGEKVGGATELLDEAWWFPAICWPDGRLQ
MSMEG_4864|M.smegmatis_MC2_155 -----KDWSFGNPAATGDGIRAGEKVGGATELLDEAWWFPAICWPDGRLQ
TH_4337|M.thermoresistible__bu -----KDWSFGNPAATGDGIRAGEKVGGATELLDEAWWFPAICWPDGRLQ
MMAR_4775|M.marinum_M -----QDWSFGNPAALGDGIRAGQRVGAAADLLDEAWWFPAIQWPDGRMQ
MUL_0346|M.ulcerans_Agy99 -----QDWSFGNPAALGDGIRAGQRVGAAADLLDEAWWFPAIQWPDGRMQ
MAV_0880|M.avium_104 -----QDWSFGNPAAMGDGIRAGQNVGAATDLLDEAWWFPAIQWPDGRMQ
Mb3567|M.bovis_AF2122/97 -----TEWTVGASANTGDGILAAEKLGAALDLMDDAWWGPTVPLV-GKPW
Rv3537|M.tuberculosis_H37Rv -----TEWTVGASANTGDGILAAEKLGAALDLMDDAWWGPTVPLV-GKPW
MAB_0622c|M.abscessus_ATCC_199 -----TEWTVGAKANTGDGILAAEKLGAALEFMEDSWWGPTVPLV-GRPW
:*:.* * **** *.:.:*.* ::::::** *:: *:
Mvan_4243|M.vanbaalenii_PYR-1 FMLNERMMPSQFVVNGDGERFINEAAPYMDFAHAMIEGQRSG--------
Mflv_2403|M.gilvum_PYR-GCK FMLNERMMPAQFIVNGAGKRFINEAAPYMDFAHAMIEGQRSG--------
MSMEG_4864|M.smegmatis_MC2_155 FMLNERMMPSQFVVNGEGKRFINEAAPYMDFAHAMIEGQQSG--------
TH_4337|M.thermoresistible__bu FMLNERMMPSQFIVNGAGRRFINEAAPYMDFAHAMIEGERAYRDSHGENG
MMAR_4775|M.marinum_M FMLNERMMPAQFIVNGAGKRFINEAAPYMDFGHAMIAGQKSG--------
MUL_0346|M.ulcerans_Agy99 FMLNERMMPAQFIVNGAGKRFINEAAPYMDFGHAMIAGQKSG--------
MAV_0880|M.avium_104 FMLNERMMPAQFIVNGAGRRFINEAAPYMDFGHAMIEGQRSG--------
Mb3567|M.bovis_AF2122/97 FALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGP-----G
Rv3537|M.tuberculosis_H37Rv FALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGP-----G
MAB_0622c|M.abscessus_ATCC_199 FALSERNSPGSIIVNSSGKRFMNESLPYVEATHRMYGGQYGQGP-----G
* *.** *..::** *.**:**: **:: * * *: .
Mvan_4243|M.vanbaalenii_PYR-1 -VTHIPCWLITDIRSFHRYVVAGHLPIPKVPFAPVPTGWKVPAAWLESGV
Mflv_2403|M.gilvum_PYR-GCK -VTHIPCWLITDIRSFHRYVVGGHLPIPKVPFAPVPTGWKVPQAWLDSGV
MSMEG_4864|M.smegmatis_MC2_155 -VTHIPCWLITDIDSFHRYVVAGHLPIPKIPFAPVPTGRKVPQAWLESGV
TH_4337|M.thermoresistible__bu TAQHIPCWLITDIRSFRRYVVAGHLPIPKIPFAPVPTGRKVPREWLESGV
MMAR_4775|M.marinum_M -VTHIPCWLVTDHRSFNRYVVAGHLPIPKIPGAPVPTGRKIPTAWLESGV
MUL_0346|M.ulcerans_Agy99 -VTHIPCWLVTDHRSFNRYVVAGHLPIPKIPGAPVPTGRKIPTAWLESGV
MAV_0880|M.avium_104 -VTHIPCWLITDHRSWNRYVIGGHLPLPKIPGAPVPTGRKIPPAWLESGV
Mb3567|M.bovis_AF2122/97 PGENIPAWLVFDQRYRDRYIFAG-----------LQPGQRIPSRWLDSGV
Rv3537|M.tuberculosis_H37Rv PGENIPAWLVFDQRYRDRYIFAG-----------LQPGQRIPSRWLDSGV
MAB_0622c|M.abscessus_ATCC_199 PGENLPAWLVFDQEYRNRYIFAG-----------LQPGQKIPSKWIESGV
::*.**: * **:..* : .* ::* *::***
Mvan_4243|M.vanbaalenii_PYR-1 VKTGSTWEELADQIGVPGTRLRGTAERFNALAAKGHDDDFNRGDSAYDNY
Mflv_2403|M.gilvum_PYR-GCK VKQGHTWEDLAREIGVPGTELRNTAERFNALAAKGHDDDFCRGDSAYDNY
MSMEG_4864|M.smegmatis_MC2_155 VQEGRTMEELATKIGVPPQNLRATAERFDQLARAGHDDDFNRGDSAYDNY
TH_4337|M.thermoresistible__bu VHEGHSWEELAAKINVPADQLRATAERFNMLAAKGHDDDFNRGDSAYDNY
MMAR_4775|M.marinum_M VKAATSWAELATKVGVPGGQLQATAERFNELAVKGHDEDFNRGDSVYDNY
MUL_0346|M.ulcerans_Agy99 VKAATSWAELATKIGVPGGQLQATAERFNELAVYGHDDDFNRGDSVYDNY
MAV_0880|M.avium_104 VKAAMTWDEMAAQIGVPAGELAETARRFNELARKGHDDDFNRGDSVYDNY
Mb3567|M.bovis_AF2122/97 IVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRY
Rv3537|M.tuberculosis_H37Rv IVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRY
MAB_0622c|M.abscessus_ATCC_199 IVKADTIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRY
: . : ::* : .:* .* * .**: :* * *:*: **.*.**.*
Mvan_4243|M.vanbaalenii_PYR-1 YGDPT-LPNPNLHPLGAPPYFAFQIILGDLGTSGGLRTDEHARVLRGDDS
Mflv_2403|M.gilvum_PYR-GCK YGDQT-LPNPNLYPLGKPPYYAFQIILGDLGTSGGLRTDEFARVLRSDDS
MSMEG_4864|M.smegmatis_MC2_155 YGDPT-LPNPNLRPLGKPPYYAFQIILGDLGTSGGLRTDEFARVLRSDET
TH_4337|M.thermoresistible__bu YGDPT-LPNPNLHPLGRPPYYAFQIILGDLGTSGGLRTDEHARVLRVDDS
MMAR_4775|M.marinum_M YGDPT-LPNPNLYPLGDPPYYAFRIVLGDLGTSGGLRTDEYARVLRPDDT
MUL_0346|M.ulcerans_Agy99 YGDPT-LPNPNLYPLGDPPYYAFRIVLGDLGTSGGLRTDEYARVLRPDDT
MAV_0880|M.avium_104 YGDPT-LPNPNLYPLGDPPYYAFRIVLGDLGTSGGLRTDEHARVLRADDT
Mb3567|M.bovis_AF2122/97 YGDPSNKPNPNLGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGS
Rv3537|M.tuberculosis_H37Rv YGDPSNKPNPNLGEVGHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGS
MAB_0622c|M.abscessus_ATCC_199 YGDPTNKPNPNLGALTKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNS
*** : ***** : .*::. ::: *****.**:*** .*.** * :
Mvan_4243|M.vanbaalenii_PYR-1 VIGGLYAVGNASAAVMGRSYAGAGATIGPAMTFGYVAARHIADSAGGTT-
Mflv_2403|M.gilvum_PYR-GCK AIPGLYAVGNASSAVMGRSYAGAGATIGPAMAFGYVAARHIAETETLTAL
MSMEG_4864|M.smegmatis_MC2_155 VVSGLYAVGNASGAVMGRSYAGAGATIGPAMTFGYVAARHAAAGESAVPN
TH_4337|M.thermoresistible__bu VVPGLYAVGNNSAAVMGRSYAGAGATIGPAMTFGYVAARHIAERAAPATT
MMAR_4775|M.marinum_M TVGGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGFVAAKHLAAQLDARRA
MUL_0346|M.ulcerans_Agy99 TVGGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGFVAAKHLAARLDARRA
MAV_0880|M.avium_104 VVPGLYAVGNTSAPVMGRSYAGAGATIGPAMTFGYVAAKHVAAQVANRAA
Mb3567|M.bovis_AF2122/97 IIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQAGKR--
Rv3537|M.tuberculosis_H37Rv IIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQAGKR--
MAB_0622c|M.abscessus_ATCC_199 VIEGLYAAGNVSTPVMGHTYAGPGATIGPAMTWGYLAALDIAGK------
: ****.** * .***::*.*.*.******::*::** . *
Mvan_4243|M.vanbaalenii_PYR-1 -DRTTTSTESPGSSRR
Mflv_2403|M.gilvum_PYR-GCK DDQSLQAPDLQGGNQ-
MSMEG_4864|M.smegmatis_MC2_155 PDNALQHRIS------
TH_4337|M.thermoresistible__bu SSPTRPSTSKSNVS--
MMAR_4775|M.marinum_M TPTTHLHGR-------
MUL_0346|M.ulcerans_Agy99 TPTTHFHGR-------
MAV_0880|M.avium_104 N--THRR---------
Mb3567|M.bovis_AF2122/97 ----------------
Rv3537|M.tuberculosis_H37Rv ----------------
MAB_0622c|M.abscessus_ATCC_199 ----------------