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MTSTAEPRTNPEYRSKNKEFGISERYTSAEFARLENEKLWPKVWQVACRVEEVPNPGDYCEYTIADETIL ITRTREGDVKALFNACQHRGMRLKEGRGSCSEIRCPSHSWCWNLDGSLNEVIDPQDFDPEAISPDALRLP ECKVEVWAGFVFINMDPDAVPLTEYLGEAGPRVENFGVHKMVCTRLRSTVIKANWKLCHEAFIETYHAIG THPQSLRYLDDTGMIFEQYGDHGMHRIAPGHMGKPSARLDGDDIDRRDVLMALMGDLAEFDFYNEEDVAQ ANALIETVMDLPEEVTVGTFFAGLRRQQAEAEGVDLSVYSDADLLAGELWNIFPNVTIPCNAGNALTLRF RPNGLNHEECIFDIFYLKVFPEGATIPPMEHEFYPDWQETKVWGTIVTQDFTNLPKWQAGVHSRGYRGPV WGRPDGNVSNLHRVISEYLAQ
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. vanbaalenii PYR-1 | Mvan_0908 | - | - | 100% (441) | Rieske (2Fe-2S) domain-containing protein |
| M. vanbaalenii PYR-1 | Mvan_4962 | - | 3e-50 | 30.43% (437) | Rieske (2Fe-2S) domain-containing protein |
| M. vanbaalenii PYR-1 | Mvan_4319 | - | 2e-36 | 25.06% (423) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 1e-25 | 32.29% (192) | dioxygenase |
| M. gilvum PYR-GCK | Mflv_1788 | - | 3e-50 | 30.82% (438) | Rieske (2Fe-2S) domain-containing protein |
| M. tuberculosis H37Rv | Rv3161c | - | 6e-26 | 32.81% (192) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 2e-15 | 24.88% (205) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 9e-53 | 31.92% (448) | dioxygenase |
| M. avium 104 | MAV_2952 | - | 4e-52 | 32.01% (428) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_5627 | - | 9e-53 | 32.55% (470) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_1336 | - | 2e-51 | 32.51% (446) | Rieske |
| M. ulcerans Agy99 | MUL_3820 | - | 2e-17 | 27.66% (188) | ring-hydroxylating dioxygenase, large terminal subunit |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 MLSTDNRAELG--------DILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv MLSTDNRAELG--------DILTDIGDYLDDNPPALSLPPAAYTSSELWQ
MSMEG_5627|M.smegmatis_MC2_155 MTDTQPR--------------------TDEALATPMTIGVEAYTSEAYAR
TH_1336|M.thermoresistible__bu MTDIDNKPEV----------------AAPEELSEPMLIPVEAYVSEEYAR
Mflv_1788|M.gilvum_PYR-GCK MTHTESDLDAAI---EVEADPDDSTGQIAEDLSDPVTIPVEAYVSEAYAR
MMAR_0307|M.marinum_M MTDHNDGRAEP--------MDAAHHDDVEATLTGAVTFGVDAYTCADYAR
Mvan_0908|M.vanbaalenii_PYR-1 MTSTAEPRTNP-----------------EYRSKNKEFGISERYTSAEFAR
MAV_2952|M.avium_104 MNATPVP--------------------VPWAVQTPDRIPKQRYYDPEFYA
MUL_3820|M.ulcerans_Agy99 MDRDQLIDLTR-------RALKLARDKTTDLAPHQHAVDAREYTSLDRHE
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
* :
Mb3186c|M.bovis_AF2122/97 LERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDG----QL
Rv3161c|M.tuberculosis_H37Rv LERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDG----QL
MSMEG_5627|M.smegmatis_MC2_155 AERDRLWRRVWQQVGRVEELPEVGSYLTYDILDDSILVVRTGPGDSGGSF
TH_1336|M.thermoresistible__bu AERDRLWRKVWLQVGRVEELPEVGSYLTYDILDDSIIVVRTGAGDSGSAF
Mflv_1788|M.gilvum_PYR-GCK AERDKLWRKVWQQVGRVEEIPEVGSYLTYDILDDSIIVVRTGEN----TF
MMAR_0307|M.marinum_M AERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRSAAD----TF
Mvan_0908|M.vanbaalenii_PYR-1 LENEKLWPKVWQVACRVEEVPNPGDYCEYTIADETILITRTREG----DV
MAV_2952|M.avium_104 LEKEMFWPRVWQMACRLEEIPKPGDFVEYEILDESVIVVRLDSQ----TV
MUL_3820|M.ulcerans_Agy99 RDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADR----AV
MAB_4370c|M.abscessus_ATCC_199 QELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNR----RI
: . .:. . : : .::: .
Mb3186c|M.bovis_AF2122/97 HALSPICRHRLMLMVEPGAGR-------IDTLTCQYHLWRYGLDG---RL
Rv3161c|M.tuberculosis_H37Rv HALSPICRHRLMLMVEPGAGR-------IDTLTCQYHLWRYGLDG---RL
MSMEG_5627|M.smegmatis_MC2_155 AAHHNVCMHRGRRLVDTPAGAKNACARTRKSFMCGFHGWTYDLTGACTHI
TH_1336|M.thermoresistible__bu AAHHNVCMHRGRRLIDTPEGAKNACGRTRRSFVCGFHGWTYGLDGACTHI
Mflv_1788|M.gilvum_PYR-GCK AAHHNVCMHRGRRLVDVPDGAKNAVGRARKSFVCGFHGWTYGLDGTCTHI
MMAR_0307|M.marinum_M KAHHNVCMHRGRRLVDIPPGAKNVCG-HKKSFVCGFHGWTYDQDGNCIHI
Mvan_0908|M.vanbaalenii_PYR-1 KALFNACQHRGMRLKEGRGSCS--------EIRCPSHSWCWNLDGSLNEV
MAV_2952|M.avium_104 RAYHNACRHRGVKLVEGNGNRR--------TFVCPFHGWCWSLDGRNTFV
MUL_3820|M.ulcerans_Agy99 RAFDNVCLHRQSRVATGCGTALR--------FTCPYHGWTYDNGGRLVGL
MAB_4370c|M.abscessus_ATCC_199 RAFLNSCRHRGARVCRADYGVTR-------GFTCTYHGWAYDLEGKLISV
* * ** : : * * * :. * :
Mb3186c|M.bovis_AF2122/97 RGAPHMAANLDFNRRECRLPQFA-VATWNGLVWINLDADA----EPIAAH
Rv3161c|M.tuberculosis_H37Rv RGAPHMAANLDFNRRECRLPQFA-VATWNGLVWINLDADA----EPIAAH
MSMEG_5627|M.smegmatis_MC2_155 REQDDWQG--TLTPENTHLPRVH-VDTWGGWLFINMDPDP----EPLADY
TH_1336|M.thermoresistible__bu REQDDWKG--ALTPENTRLAPVN-VDTWGGWLWINMDPAC----EPLADY
Mflv_1788|M.gilvum_PYR-GCK REQDDWKG--TLTPRNTHLAPVQ-VDTWGGWLFVNMDPDC----EPLADY
MMAR_0307|M.marinum_M PEKPDWQG--ALTPENTHLAAVN-VDTWGGWIWINMDPAA----QPLRQY
Mvan_0908|M.vanbaalenii_PYR-1 IDPQDFDP-EAISPDALRLPECK-VEVWAGFVFINMDPDA----VPLTEY
MAV_2952|M.avium_104 LRPETFAQ-ENLAAADLRLVSVR-CELWGGCAWINLDDDA----PALRDW
MUL_3820|M.ulcerans_Agy99 PGREGFPG---IAVGSDGLTELP-ATEFAGFLWVSLHPAAR---LDVPAH
MAB_4370c|M.abscessus_ATCC_199 PNEEGYPD--SFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLGDM
: * : * : . . :
Mb3186c|M.bovis_AF2122/97 LDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQ
Rv3161c|M.tuberculosis_H37Rv LDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGH-ENYHVLGLHRQ
MSMEG_5627|M.smegmatis_MC2_155 LFPAAKILDPFGLENMRYKWRKWLYFDCNWKVALEAFN-ETYHVFTTHPE
TH_1336|M.thermoresistible__bu LFPADKLLDHFGLENMRYKWRRWLDFDCNWKVALEAFN-ETYHVYTTHPE
Mflv_1788|M.gilvum_PYR-GCK LFPASKILEPFGLENMRYKWRKWLYFDCNWKVAMEAFN-ETYHVFTTHPE
MMAR_0307|M.marinum_M LEPAATMLDPFRLQDMRCKWRKWLTFQCNWKVAMEAFN-ETYHVATTHPQ
Mvan_0908|M.vanbaalenii_PYR-1 LGEAGPRVENFGVHKMVCTRLRSTVIKANWKLCHEAFI-ETYHAIGTHPQ
MAV_2952|M.avium_104 MEPFASTYDAWRVESLRVEWWQSCRLPVNWKLATAAFM-EGYHVPQTHPQ
MUL_3820|M.ulcerans_Agy99 LGPLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFA-ENYHFATVHRR
MAB_4370c|M.abscessus_ATCC_199 AWYMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSHAS
:**:. : ** *
Mb3186c|M.bovis_AF2122/97 T-----LEPFVPGGGDLDVRQYSR--------WALRLRVPFTVPVEAKSL
Rv3161c|M.tuberculosis_H37Rv T-----LEPFVPGGGDLDVRQYSR--------WALRLRVPFTVPVEAKSL
MSMEG_5627|M.smegmatis_MC2_155 FNKFGEFKGWAKAQGKHSNIGYDAPKDMEATKSKIRLGTGDDPRVSTAEM
TH_1336|M.thermoresistible__bu FNRFGEFKGWAKAQGRHSNIGYDAPKDLEATKSKIRLGTGDDPRVSTAEM
Mflv_1788|M.gilvum_PYR-GCK FNKFGEFKGWAKAQGRHSNIGYDAPKGMDETKSKIRLGTGD-PRISTAEM
MMAR_0307|M.marinum_M FNKFGNFRGWAAAHGRHSNIGYDAPKDLAQTKSKIRLGTGADPRVSTAEM
Mvan_0908|M.vanbaalenii_PYR-1 SLRYLDDTGMIFEQYGDHGMHRIAPGHMGKPSARLDGDDIDRRDVLMALM
MAV_2952|M.avium_104 LLPGAQTGEPSAAVHPVVASSLYFMRTLGEGMAGMTHQNDIRIAEGLQTM
MUL_3820|M.ulcerans_Agy99 T---------FATIARSNCTVFDSYG----PHHRLIFPLNTILELENVPE
MAB_4370c|M.abscessus_ATCC_199 ALMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDARVVHQW
Mb3186c|M.bovis_AF2122/97 QLNEVQKSNLVVLWTFPNSALAIAGER-----------------------
Rv3161c|M.tuberculosis_H37Rv QLNEVQKSNLVVLWTFPNSALAIAGER-----------------------
MSMEG_5627|M.smegmatis_MC2_155 QMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDEA
TH_1336|M.thermoresistible__bu QMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDEA
Mflv_1788|M.gilvum_PYR-GCK QVYTMEETNATTTQTLVNAAKRLVDELPEGTPADQVLQHWLASARRDDEA
MMAR_0307|M.marinum_M QRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHWLTSARRDDAA
Mvan_0908|M.vanbaalenii_PYR-1 GDLAEFDFYNEEDVAQANALIETVMDLPEEVTVG----TFFAGLRRQQAE
MAV_2952|M.avium_104 RLPSDPAAAMAAWRSALNDAVVDWHRAR----------------------
MUL_3820|M.ulcerans_Agy99 GQWDPFHHMVVIYALFPNVVLSVTIAN-----------------------
MAB_4370c|M.abscessus_ATCC_199 TDYGALRERLGEARVQGPLTNGHATVFPNLSYLP---------------V
Mb3186c|M.bovis_AF2122/97 --VVWFGFIPQSIDR----------VQVLGGVLTTPELAADAAATAQTSQ
Rv3161c|M.tuberculosis_H37Rv --VVWFGFIPQSIDR----------VQVLGGVLTTPELAADAAATAQTSQ
MSMEG_5627|M.smegmatis_MC2_155 RGVIWPTIPADILGQAGTAWQIFPNFQIGQGLTTALCYSAKPHPSYNPDK
TH_1336|M.thermoresistible__bu RGVIWPTIPPEVLGEAGTAWQIFPNFQIGQGLTTALCYSARPHPSYDPNK
Mflv_1788|M.gilvum_PYR-GCK RGVIWPTIPPDILGQSGTAWQVFPNFQIGQGLTSALCYSARPDPSYDPNK
MMAR_0307|M.marinum_M RGVLWPTIDPEHLAQSGTAWQIFPNFQIGQGLTTALCYSARPHG-YDPDQ
Mvan_0908|M.vanbaalenii_PYR-1 AEGVDLSVYSDADLLAGELWNIFPNVTIPCNAGNALTLRFRPNG-LNHEE
MAV_2952|M.avium_104 GSGMPDLNELDRRGITDAIGFAFPHHFILPTYSSASSYRIRPLG---PEE
MUL_3820|M.ulcerans_Agy99 -GELFRIYPGDRPGSS------------ITVHQNSTPLDLSDESVATGAQ
MAB_4370c|M.abscessus_ATCC_199 NGSVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFG----PT
:
Mb3186c|M.bovis_AF2122/97 FVMAMINDEDRLG---------------------LEAVQVGAGSRFAERG
Rv3161c|M.tuberculosis_H37Rv FVMAMINDEDRLG---------------------LEAVQVGAGSRFAERG
MSMEG_5627|M.smegmatis_MC2_155 CIFEVATFELFPKGQEP---RTEWEYTPADSPNWLSVLPQDFSNMAAVQQ
TH_1336|M.thermoresistible__bu CLFEVSVLELFPKGEEP---QTEWEYTPVGDPRWLSVLPQDFSNMAAVQQ
Mflv_1788|M.gilvum_PYR-GCK CIFEVAVFELYPKGEEP---GTEWAYTPKDSPNWLSVLPQDFSNMAAVQQ
MMAR_0307|M.marinum_M CVFEASCYELYPKGEEP---HTEWVYAPPDDPGWRSVLPQDFSNMAAVQQ
Mvan_0908|M.vanbaalenii_PYR-1 CIFDIFYLKVFPEGATIPPMEHEFYPDWQETKVWGTIVTQDFTNLPKWQA
MAV_2952|M.avium_104 TLFEIWSLTRLPGDASAG-KPTPPEPMAPDDPRWPPIPAQDFSNLPRQQK
MUL_3820|M.ulcerans_Agy99 AVFDYAH---------------------------ATVRDEDYRLAETLQT
MAB_4370c|M.abscessus_ATCC_199 GIFEQDDGENWSEVQSIS--------------RGFVTNSVALNYQMGLDS
::
Mb3186c|M.bovis_AF2122/97 HLSSKEWPG----------MLAFYRNLAMALVGDHPGAS-----------
Rv3161c|M.tuberculosis_H37Rv HLSSKEWPG----------MLAFYRNLAMALVGDHPGAS-----------
MSMEG_5627|M.smegmatis_MC2_155 GMKSMGFPGTKPNPYRERSTVNLHYQLSKYMGTGEPRDLQENA-------
TH_1336|M.thermoresistible__bu GMKSLGFPGPRPNPYRERSTVNLHHQLSKYMGTGEPREI-----------
Mflv_1788|M.gilvum_PYR-GCK GMKSAGFPGTLPNPYRERSTVNLHHQLSKYMGTGDPREI-----------
MMAR_0307|M.marinum_M GMKSGGFGGPKPNPYMERSTANLHRNLAEYMGTGAPREL-----------
Mvan_0908|M.vanbaalenii_PYR-1 GVHSRGYRGPVWGR-PDGNVSNLHRVISEYLAQ-----------------
MAV_2952|M.avium_104 GLHSKSFEFMRLSDRIEGLISNFERVIDGFLAGLRYDALLPAIHKTNTTI
MUL_3820|M.ulcerans_Agy99 NLESGARQRLVFGR---NEPGLQHRHITWAQALETPG-------------
MAB_4370c|M.abscessus_ATCC_199 EREDGRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV---------
.
Mb3186c|M.bovis_AF2122/97 ---------------
Rv3161c|M.tuberculosis_H37Rv ---------------
MSMEG_5627|M.smegmatis_MC2_155 ---------------
TH_1336|M.thermoresistible__bu ---------------
Mflv_1788|M.gilvum_PYR-GCK ---------------
MMAR_0307|M.marinum_M ---------------
Mvan_0908|M.vanbaalenii_PYR-1 ---------------
MAV_2952|M.avium_104 DVPVVDLGFGAVEAR
MUL_3820|M.ulcerans_Agy99 ---------------
MAB_4370c|M.abscessus_ATCC_199 ---------------