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VTHTESDLDAAIEVEADPDDSTGQIAEDLSDPVTIPVEAYVSEAYARAERDKLWRKVWQQVGRVEEIPEV GSYLTYDILDDSIIVVRTGENTFAAHHNVCMHRGRRLVDVPDGAKNAVGRARKSFVCGFHGWTYGLDGTC THIREQDDWKGTLTPRNTHLAPVQVDTWGGWLFVNMDPDCEPLADYLFPASKILEPFGLENMRYKWRKWL YFDCNWKVAMEAFNETYHVFTTHPEFNKFGEFKGWAKAQGRHSNIGYDAPKGMDETKSKIRLGTGDPRIS TAEMQVYTMEETNATTTQTLVNAAKRLVDELPEGTPADQVLQHWLASARRDDEARGVIWPTIPPDILGQS GTAWQVFPNFQIGQGLTSALCYSARPDPSYDPNKCIFEVAVFELYPKGEEPGTEWAYTPKDSPNWLSVLP QDFSNMAAVQQGMKSAGFPGTLPNPYRERSTVNLHHQLSKYMGTGDPREI
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_1788 | - | - | 100% (470) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2328 | - | 4e-42 | 27.38% (442) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_2470 | - | 8e-32 | 35.43% (223) | Rieske (2Fe-2S) domain-containing protein |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 7e-23 | 28.18% (220) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 5e-23 | 28.18% (220) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 3e-10 | 24.87% (197) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_0307 | - | 0.0 | 71.83% (458) | dioxygenase |
| M. avium 104 | MAV_5207 | - | 0.0 | 86.28% (452) | Rieske (2Fe-2S) domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_5627 | - | 0.0 | 83.55% (456) | Rieske (2Fe-2S) domain-containing protein |
| M. thermoresistible (build 8) | TH_1336 | - | 0.0 | 83.96% (449) | Rieske |
| M. ulcerans Agy99 | MUL_3820 | - | 5e-14 | 28.41% (176) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_4962 | - | 0.0 | 91.18% (465) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3186c|M.bovis_AF2122/97 ----MLSTDNRAELGDILTDIG-DYLDDNP-------PALSLPPAAYTSS
Rv3161c|M.tuberculosis_H37Rv ----MLSTDNRAELGDILTDIG-DYLDDNP-------PALSLPPAAYTSS
Mflv_1788|M.gilvum_PYR-GCK MTHTESDLDAAIEVEADPDDSTGQIAEDLS-------DPVTIPVEAYVSE
Mvan_4962|M.vanbaalenii_PYR-1 ----MTDLDNQPEIDA---------AEELS-------ESMTIPVEAYISE
MAV_5207|M.avium_104 ----MTDVAKDANSAV---------AEELS-------TPMTIGVEAYISE
TH_1336|M.thermoresistible__bu ----MTDIDNKPEVAA---------PEELS-------EPMLIPVEAYVSE
MSMEG_5627|M.smegmatis_MC2_155 ----MTDTQPRT-------------DEALA-------TPMTIGVEAYTSE
MMAR_0307|M.marinum_M ----MTDHNDGRAEPMDAAHHD-DVEATLT-------GAVTFGVDAYTCA
MUL_3820|M.ulcerans_Agy99 -----MDRDQLIDLTRRALKLARDKTTDLAP------HQHAVDAREYTSL
MAB_4370c|M.abscessus_ATCC_199 ----MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDG
. . :
Mb3186c|M.bovis_AF2122/97 ELWQLERERIFNRSWMLVAHVDQLAKTGDYVTVSVAGEPVMVVRDVDG--
Rv3161c|M.tuberculosis_H37Rv ELWQLERERIFNRSWMLVAHVDQVAKTGDYVTVSVAGEPVMVVRDVDG--
Mflv_1788|M.gilvum_PYR-GCK AYARAERDKLWRKVWQQVGRVEEIPEVGSYLTYDILDDSIIVVRTGEN--
Mvan_4962|M.vanbaalenii_PYR-1 EYARAERDRLWRKVWQQVGRVEEIPEVGSYLTYDILDDSIIVVRTGAD--
MAV_5207|M.avium_104 DYARAERDKLWRKVWQQVGRVEELPEVGSYLTYDILDDSIIVVRTGAN--
TH_1336|M.thermoresistible__bu EYARAERDRLWRKVWLQVGRVEELPEVGSYLTYDILDDSIIVVRTGAGDS
MSMEG_5627|M.smegmatis_MC2_155 AYARAERDRLWRRVWQQVGRVEELPEVGSYLTYDILDDSILVVRTGPGDS
MMAR_0307|M.marinum_M DYARAERDKLWRKVWQQVGRVEDLPKVGSYLTYDILDDSIVVVRSAAD--
MUL_3820|M.ulcerans_Agy99 DRHERDRAMVLD-SPQLVGYSSELPGPDTYRTKTIMGRSILLTRAADR--
MAB_4370c|M.abscessus_ATCC_199 ELYRQELRRVFAPSWLFLAHADQFHRPGDFFTTYMGEDPVIVSMDKNR--
. : : :. .:. . : * : .:::
Mb3186c|M.bovis_AF2122/97 --QLHALSPICRHRLMLMVEPGAG-------RIDTLTCQYHLWRYGLDGR
Rv3161c|M.tuberculosis_H37Rv --QLHALSPICRHRLMLMVEPGAG-------RIDTLTCQYHLWRYGLDGR
Mflv_1788|M.gilvum_PYR-GCK --TFAAHHNVCMHRGRRLVDVPDGAKNAVGRARKSFVCGFHGWTYGLDGT
Mvan_4962|M.vanbaalenii_PYR-1 --TFAAHHNVCMHRGRRLVDTPEGCKNAVGRARKSFVCGFHGWTYGLDGT
MAV_5207|M.avium_104 --EFRAHHNVCMHRGRRLIDTPEGAKNALGRTRKSFVCGFHGWTYGLDGA
TH_1336|M.thermoresistible__bu GSAFAAHHNVCMHRGRRLIDTPEGAKNACGRTRRSFVCGFHGWTYGLDGA
MSMEG_5627|M.smegmatis_MC2_155 GGSFAAHHNVCMHRGRRLVDTPAGAKNACARTRKSFMCGFHGWTYDLTGA
MMAR_0307|M.marinum_M --TFKAHHNVCMHRGRRLVDIPPGAKNVCG-HKKSFVCGFHGWTYDQDGN
MUL_3820|M.ulcerans_Agy99 --AVRAFDNVCLHR-QSRVATGCG-------TALRFTCPYHGWTYDNGGR
MAB_4370c|M.abscessus_ATCC_199 --RIRAFLNSCRHRGARVCRADYG-------VTRGFTCTYHGWAYDLEGK
. * * ** * : * :* * *. *
Mb3186c|M.bovis_AF2122/97 LRGAP-HMAANLDFNRRECRLPQFA-VATWNGLVWINLDADA----EPIA
Rv3161c|M.tuberculosis_H37Rv LRGAP-HMAANLDFNRRECRLPQFA-VATWNGLVWINLDADA----EPIA
Mflv_1788|M.gilvum_PYR-GCK CTHIREQDDWKGTLTPRNTHLAPVQ-VDTWGGWLFVNMDPDC----EPLA
Mvan_4962|M.vanbaalenii_PYR-1 CTHIREQDDWKGKLTPRNTHLAPVR-VDTWGGWLFVNMDPDC----EPLA
MAV_5207|M.avium_104 CTHIREQQDWRQALTPDNTHLRPVR-VDTWGGWLWINMDPDC----EPLA
TH_1336|M.thermoresistible__bu CTHIREQDDWKGALTPENTRLAPVN-VDTWGGWLWINMDPAC----EPLA
MSMEG_5627|M.smegmatis_MC2_155 CTHIREQDDWQGTLTPENTHLPRVH-VDTWGGWLFINMDPDP----EPLA
MMAR_0307|M.marinum_M CIHIPEKPDWQGALTPENTHLAAVN-VDTWGGWIWINMDPAA----QPLR
MUL_3820|M.ulcerans_Agy99 LVGLPGREGFPG-IAVGSDGLTELP-ATEFAGFLWVSLHPAAR---LDVP
MAB_4370c|M.abscessus_ATCC_199 LISVPNEEGYPDSFSTDDWGLVEVPHVDSYHGLIFGTWNPAPVGLRESLG
. : . * . . : * :: . .. :
Mb3186c|M.bovis_AF2122/97 AHLDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGHENYHVLGLHR
Rv3161c|M.tuberculosis_H37Rv AHLDLTDDEFAGYRLGEMVQVESWSHEWRANWKVAAENGHENYHVLGLHR
Mflv_1788|M.gilvum_PYR-GCK DYLFPASKILEPFGLENMRYKWRKWLYFDCNWKVAMEAFNETYHVFTTHP
Mvan_4962|M.vanbaalenii_PYR-1 DYLFPAAKILDPFGLENMRYKWRKWLYFDCNWKVALEAFNETYHVFTTHP
MAV_5207|M.avium_104 DYLFPAAKILEPFGLENMRYKWRKWLYFDCNWKVALEAFNETYHVYTTHP
TH_1336|M.thermoresistible__bu DYLFPADKLLDHFGLENMRYKWRRWLDFDCNWKVALEAFNETYHVYTTHP
MSMEG_5627|M.smegmatis_MC2_155 DYLFPAAKILDPFGLENMRYKWRKWLYFDCNWKVALEAFNETYHVFTTHP
MMAR_0307|M.marinum_M QYLEPAATMLDPFRLQDMRCKWRKWLTFQCNWKVAMEAFNETYHVATTHP
MUL_3820|M.ulcerans_Agy99 AHLGPLAGELQSWGIGRWSPLGEKVLGSPIDWKLATDTFAENYHFATVHR
MAB_4370c|M.abscessus_ATCC_199 DMAWYMDAMLDHDSEGTVVVGGVHKWVLDGNWKLAAEQFATDWYHVNMSH
: :**:* : ::
Mb3186c|M.bovis_AF2122/97 QT-----LEPFVPGGGDLDVRQYSR--------WALRLRVPFTVPVEAKS
Rv3161c|M.tuberculosis_H37Rv QT-----LEPFVPGGGDLDVRQYSR--------WALRLRVPFTVPVEAKS
Mflv_1788|M.gilvum_PYR-GCK EFNKFGEFKGWAKAQGRHSNIGYDAPKGMDETKSKIRLGTG-DPRISTAE
Mvan_4962|M.vanbaalenii_PYR-1 EFNRFGEFKGWAKAQGKHSNIGYDAPKGMDETKSKIRLGTG-DPRISTAE
MAV_5207|M.avium_104 EFNKFGEFKGWAKAQGRHSNIGYDAPEDMEATKSKIRLGIGADPRVSTAE
TH_1336|M.thermoresistible__bu EFNRFGEFKGWAKAQGRHSNIGYDAPKDLEATKSKIRLGTGDDPRVSTAE
MSMEG_5627|M.smegmatis_MC2_155 EFNKFGEFKGWAKAQGKHSNIGYDAPKDMEATKSKIRLGTGDDPRVSTAE
MMAR_0307|M.marinum_M QFNKFGNFRGWAAAHGRHSNIGYDAPKDLAQTKSKIRLGTGADPRVSTAE
MUL_3820|M.ulcerans_Agy99 RT---------FATIARSNCTVFDSYG----PHHRLIFPLNTILELENVP
MAB_4370c|M.abscessus_ATCC_199 AS----ALMVMAPQGKAPKDEIVHRTGRQYVDPNGHGAGFPVHPKNRFDA
.
Mb3186c|M.bovis_AF2122/97 LQLNEVQKSNLVVLWTFPNSALAIAGER----------------------
Rv3161c|M.tuberculosis_H37Rv LQLNEVQKSNLVVLWTFPNSALAIAGER----------------------
Mflv_1788|M.gilvum_PYR-GCK MQVYTMEETNATTTQTLVNAAKRLVDELPEGTPADQVLQHWLASARRDDE
Mvan_4962|M.vanbaalenii_PYR-1 MQVYTMEETNATTTETLVNAAKRLVDELPEGTPADQVLQHWLASARRDDE
MAV_5207|M.avium_104 MQVYTMEETNATTTQTLVNAAKRLVDELPEGTPADKVLEHWLASARRDDE
TH_1336|M.thermoresistible__bu MQMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDE
MSMEG_5627|M.smegmatis_MC2_155 MQMYTWEQTNATTTETLVNAAKRLVDELPEGTPPDKVLEHWLASARRDDE
MMAR_0307|M.marinum_M MQRYTLEETNATTTRTLVDAALRLVEELPEDAPAGQVLDHWLTSARRDDA
MUL_3820|M.ulcerans_Agy99 EGQWDPFHHMVVIYALFPNVVLSVTIAN----------------------
MAB_4370c|M.abscessus_ATCC_199 RVVHQWTDYGALRERLGEARVQGPLTNG----------------------
. .
Mb3186c|M.bovis_AF2122/97 ---VVWFGFIPQSIDR----------VQVLGGVLTTPELAADAAATAQTS
Rv3161c|M.tuberculosis_H37Rv ---VVWFGFIPQSIDR----------VQVLGGVLTTPELAADAAATAQTS
Mflv_1788|M.gilvum_PYR-GCK ARGVIWPTIPPDILGQSGTAWQVFPNFQIGQGLTSALCYSARPDPSYDPN
Mvan_4962|M.vanbaalenii_PYR-1 ARGVIWPTIPPDILGQSGTAWQIFPNFQIGQGLTSALCYSARPDPSYDPN
MAV_5207|M.avium_104 ARGVIWPTIPPDILGQAGTAWQIFPNFQIGQGLTSALCYGARPHPSYNPD
TH_1336|M.thermoresistible__bu ARGVIWPTIPPEVLGEAGTAWQIFPNFQIGQGLTTALCYSARPHPSYDPN
MSMEG_5627|M.smegmatis_MC2_155 ARGVIWPTIPADILGQAGTAWQIFPNFQIGQGLTTALCYSAKPHPSYNPD
MMAR_0307|M.marinum_M ARGVLWPTIDPEHLAQSGTAWQIFPNFQIGQGLTTALCYSARPHG-YDPD
MUL_3820|M.ulcerans_Agy99 --GELFRIYPGDRPGSS----------ITVHQNSTPLDLSDESVATGAQA
MAB_4370c|M.abscessus_ATCC_199 -HATVFPNLSYLPVNGSVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQR
:: : .
Mb3186c|M.bovis_AF2122/97 QFVMAMINDEDRLG------------------LEAVQVGAGSRFAERGHL
Rv3161c|M.tuberculosis_H37Rv QFVMAMINDEDRLG------------------LEAVQVGAGSRFAERGHL
Mflv_1788|M.gilvum_PYR-GCK KCIFEVAVFELYPKGEEPGTEWAYTPKDSPNWLSVLPQDFSNMAAVQQGM
Mvan_4962|M.vanbaalenii_PYR-1 KCIFEVAVFELYPKGGEPETEWVYTPKDSPNWLSVLPQDFSNMAAVQQGM
MAV_5207|M.avium_104 KCIFEVSVFELYPKGEEPQTEWEYTPVGDPRWRSVLPQDFSNMAAVQQGM
TH_1336|M.thermoresistible__bu KCLFEVSVLELFPKGEEPQTEWEYTPVGDPRWLSVLPQDFSNMAAVQQGM
MSMEG_5627|M.smegmatis_MC2_155 KCIFEVATFELFPKGQEPRTEWEYTPADSPNWLSVLPQDFSNMAAVQQGM
MMAR_0307|M.marinum_M QCVFEASCYELYPKGEEPHTEWVYAPPDDPGWRSVLPQDFSNMAAVQQGM
MUL_3820|M.ulcerans_Agy99 VFDYAHATVR-----------------DEDYRLAETLQTNLESGARQRLV
MAB_4370c|M.abscessus_ATCC_199 --LYNLRTFGPTGIFEQDDGENWSEVQSISRGFVTNSVALNYQMGLDSER
.
Mb3186c|M.bovis_AF2122/97 SSKEWPG----------MLAFYRNLAMALVGDHPGAS--------
Rv3161c|M.tuberculosis_H37Rv SSKEWPG----------MLAFYRNLAMALVGDHPGAS--------
Mflv_1788|M.gilvum_PYR-GCK KSAGFPGTLPNPYRERSTVNLHHQLSKYMGTGDPREI--------
Mvan_4962|M.vanbaalenii_PYR-1 KSAGFPGTLPNPYRERSTVNLHHQLSKYMGTGEPREL--------
MAV_5207|M.avium_104 KSLGFPGTKPNPYRERSTVNLHYQLSRYMGTGAPRELSDKEHPLA
TH_1336|M.thermoresistible__bu KSLGFPGPRPNPYRERSTVNLHHQLSKYMGTGEPREI--------
MSMEG_5627|M.smegmatis_MC2_155 KSMGFPGTKPNPYRERSTVNLHYQLSKYMGTGEPRDLQENA----
MMAR_0307|M.marinum_M KSGGFGGPKPNPYMERSTANLHRNLAEYMGTGAPREL--------
MUL_3820|M.ulcerans_Agy99 FGRNEPG------------LQHRHITWAQALETPG----------
MAB_4370c|M.abscessus_ATCC_199 EDGRHPGLTSELYSDAAGRSFYARWRDLMNTKAWHEEHV------
. * : .