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TITAGSPLTRLSEQELEKLAKEFDAIHDEVFAELGDRDRQYIKTVISAQRQIVVAGRVLLLASRSRTALV LGTACLGLAKILENMELGHNILHGQWDWMNDPDIHSSVWDWDTASSAKAWKHSHNYIHHTYTNILGKDKD LGYEIMRIDPNQKWRPSNLGQPFYNFLLTVLFEWGVAVHDTDVESLFRGEKKLKDLKEDFKSIGGKARQQ IVKDYIGWPLVSAGAFGLAQLALRGRLDQPSHSRIGKLLRRTTGNRRVGGVATLLDRYASGVESTYLRTL GADALANLIRNVWAHAIIFCGHFPDQTWTFTEEEVENETRGGWYVRQLLGAANIEGSPLFHVLSGNLGYQ VEHHLFPDMPSSRYAEIAPKVKDICERYELPYNSGRFGKQWFTVHRTIFRLALPGGTPRPKPGPYRSPER GRSPQGVSEAERFRSRVPAEHPAAGPEHESGGVAVAPPARGND*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_5246 | - | - | 100% (464) | fatty acid desaturase |
| M. gilvum PYR-GCK | Mflv_4698 | - | e-103 | 43.21% (405) | fatty acid desaturase |
| M. gilvum PYR-GCK | Mflv_4814 | - | e-102 | 45.69% (394) | fatty acid desaturase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3258c | - | 1e-97 | 40.49% (405) | linoleoyl-CoA desaturase |
| M. tuberculosis H37Rv | Rv3229c | - | 1e-97 | 40.49% (405) | linoleoyl-CoA desaturase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_3548c | - | 2e-99 | 41.98% (405) | putative fatty acid desaturase |
| M. marinum M | MMAR_0336 | desA3_2 | 1e-102 | 46.84% (395) | linoleoyl-CoA desaturase, DesA3_2 |
| M. avium 104 | MAV_5167 | - | 1e-101 | 46.45% (394) | Fatty acid desaturase |
| M. smegmatis MC2 155 | MSMEG_6835 | - | 0.0 | 85.78% (464) | Fatty acid desaturase |
| M. thermoresistible (build 8) | TH_0650 | desA3 | 4e-97 | 40.99% (405) | POSSIBLE LINOLEOYL-CoA DESATURASE (DELTA(6)-DESATURASE) |
| M. ulcerans Agy99 | MUL_4785 | desA3_2 | 1e-100 | 46.33% (395) | linoleoyl-CoA desaturase, DesA3_2 |
| M. vanbaalenii PYR-1 | Mvan_1768 | - | 1e-104 | 42.08% (442) | fatty acid desaturase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb3258c|M.bovis_AF2122/97 MAITDVDVFAH-----------LTDADIENLAAELDAIRRDVEESRGERD
Rv3229c|M.tuberculosis_H37Rv MAITDVDVFAH-----------LTDADIENLAAELDAIRRDVEESRGERD
TH_0650|M.thermoresistible__bu MAITDVPAYAH-----------LTEVDIDALAVELDAIRRDIEDSLGERD
Mvan_1768|M.vanbaalenii_PYR-1 MAITDVPQFAH-----------LTEADVENLGRELDAIRQDIEDSRGERD
MAB_3548c|M.abscessus_ATCC_199 MAISDIQEFAH-----------LTEADVEALGHELDAIRRDVEESLGAAD
MMAR_0336|M.marinum_M MGNNNIATIAETADTATRANFALTPEQAEAFGRELDAIKERVIADLGEAD
MUL_4785|M.ulcerans_Agy99 MGNNNIATIAETADTATRANFALTPEQAEAFGRELDAIKERVIADLGEAD
MAV_5167|M.avium_104 MTHNKIT---------------LTPEQAEEFGRELDALKERVMADLGEKD
Mflv_5246|M.gilvum_PYR-GCK MTITAGSPLTR-----------LSEQELEKLAKEFDAIHDEVFAELGDRD
MSMEG_6835|M.smegmatis_MC2_155 MVITAPSPLHR-----------LSEQDLEKLAKEFDAIHDEVFAELGERD
* . *: : : :. *:**:: : . * *
Mb3258c|M.bovis_AF2122/97 ARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVAKIIENMEIG
Rv3229c|M.tuberculosis_H37Rv ARYIRRTIAAQRALEVSGRLLLAGSSRRLAWWTGALTLGVAKIIENMEIG
TH_0650|M.thermoresistible__bu ARYIRRTIATQRGLEVAGRLLLAASSRRSAWWAGAVTLAAAKIIENMEIG
Mvan_1768|M.vanbaalenii_PYR-1 ARYIRRTIAIQRGLEVTGRLLLAGSAKRRFWWAGTATLGVAKIIENMEIG
MAB_3548c|M.abscessus_ATCC_199 ARYIRRTIAAQRTLELTGRILLAASKKRSAWWAGAVTLGVAKIVENMEIG
MMAR_0336|M.marinum_M ADYIRRVIKAQRALEVGGRALLFAGILPPAWLAGTAMLGLSKILDNMEIG
MUL_4785|M.ulcerans_Agy99 ADYIRRVIKAQRALEVGGRALLFAGILPPTWLAGTAMLGLSKILDNMEIG
MAV_5167|M.avium_104 ADYIRRVIKAQRALEVGGRALLF---LPPAWLLGTTMLGLSKILDNMEIG
Mflv_5246|M.gilvum_PYR-GCK RQYIKTVISAQRQIVVAGRVLLLASRSRTALVLGTACLGLAKILENMELG
MSMEG_6835|M.smegmatis_MC2_155 RHYIKTVISVQRQIIVAGRVLLLASRSRTAWVLGTACLSMAKILENMELG
**: .* ** : : ** ** *: *. :**::***:*
Mb3258c|M.bovis_AF2122/97 HNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMD
Rv3229c|M.tuberculosis_H37Rv HNVMHGQWDWMNDPEIHSSTWEWDMSGSSKHWRYTHNFVHHKYTNILGMD
TH_0650|M.thermoresistible__bu HNVMHGQWDWMNDPEIHSSTWEWDMTGASKHWRFTHNFMHHKYTNILGMD
Mvan_1768|M.vanbaalenii_PYR-1 HNVMHGQWDWMNDPEIHSSSWEWDMSGASKHWRFTHNFMHHKYTNILGMD
MAB_3548c|M.abscessus_ATCC_199 HNVMHGQWDWMNDPEIHSSSWEWDMVDPAEHWKQTHNYIHHKYTNVLGMD
MMAR_0336|M.marinum_M HNVMHGQYDWMRDPAISGRSFEWDTACPADQWRHSHNYMHHTHTNIVGMD
MUL_4785|M.ulcerans_Agy99 HNVMHDQYDWMRDPAISGRSFEWDTACPADQWRHSHNYMHHTHTNILGMD
MAV_5167|M.avium_104 HNVMHGQYDWMRDPAISGRSFEWDTACPADQWRHSHNYMHHTHTNIVGMD
Mflv_5246|M.gilvum_PYR-GCK HNILHGQWDWMNDPDIHSSVWDWDTASSAKAWKHSHNYIHHTYTNILGKD
MSMEG_6835|M.smegmatis_MC2_155 HNILHGQWDWMNDPDVHSSVWDWDTASSAKAWKHSHNYIHHTYTNILGKD
**::*.*:***.** : . ::** .:. *: :**::**.:**::* *
Mb3258c|M.bovis_AF2122/97 DDVGYGMLRVTRDQRWKRYNIFNVVWNTILAIGFEWGVALQHLEIGKIFK
Rv3229c|M.tuberculosis_H37Rv DDVGYGMLRVTRDQRWKRYNIFNVVWNTILAIGFEWGVALQHLEIGKIFK
TH_0650|M.thermoresistible__bu DDVGYGLLRVTRDQKWKPFNLGNLLYNTLLALAFEWGVGLQHVELGKIFK
Mvan_1768|M.vanbaalenii_PYR-1 DDVGYGVIRVTRDQRWRPMNLLNLFFNTLLSVAFEWGVGLQHLELGKIFK
MAB_3548c|M.abscessus_ATCC_199 DDVGYGLLRVTRDKKWKPFNLGNLLYNTLLMLLFEWGVGTQHLELGKVAQ
MMAR_0336|M.marinum_M RDIGYGIIRMSEDQPWQPYYLGNPVYAFLLMVLFQYGVALHELETERIRS
MUL_4785|M.ulcerans_Agy99 RDIGYGIIRMSEDQPWQPYYLGNPVYAFLLMVLFQYGVALHELETERIRS
MAV_5167|M.avium_104 RDIGYGILRMSEDQPWQPYFLGNPLYAFLLMVLFQYGVALHELETERIRS
Mflv_5246|M.gilvum_PYR-GCK KDLGYEIMRIDPNQKWRPSNLGQPFYNFLLTVLFEWGVAVHDTDVESLFR
MSMEG_6835|M.smegmatis_MC2_155 KDLGYEIMRIDPHQKWRPGNLGQPLYNLLLMLLFEWGVAVHDTDIEGLIR
*:** ::*: .: *: : : .: :* : *::**. :. : :
Mb3258c|M.bovis_AF2122/97 GRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLS-------------
Rv3229c|M.tuberculosis_H37Rv GRADREAAKTRLREFSAKAGRQVFKDYVAFPALTSLS-------------
TH_0650|M.thermoresistible__bu RRHDPDESRVRVREFLRKAGRQVFKDYVAYPALSSLS-------------
Mvan_1768|M.vanbaalenii_PYR-1 GRDDRKATMGRVKEFGVKAGQQVVKDYVAYPAITSLS-------------
MAB_3548c|M.abscessus_ATCC_199 GRADKEEFRVKRQAALTKMGKQVAKDYVAFPALSALS-------------
MMAR_0336|M.marinum_M GEITLRDKRETLMEIWKKTRRQTLKDYVAFPLLAG---------------
MUL_4785|M.ulcerans_Agy99 GEITLRDKRETLTEIWKKTRRQTLKDYVAFPLLAG---------------
MAV_5167|M.avium_104 GEIRLADKREVLREIWQKTRRQTLKDYVAFPLLAG---------------
Mflv_5246|M.gilvum_PYR-GCK GEKKLKDLKEDFKSIGGKARQQIVKDYIGWPLVSAGAFGLAQLALRGRLD
MSMEG_6835|M.smegmatis_MC2_155 GQKTFADLKDDFKEIGVKARQQIVKDYLGWPMISAGAFALAQLALRGRLP
. * :* ***:.:* ::.
Mb3258c|M.bovis_AF2122/97 ---------------------------------PGATYRSTLTANVVANV
Rv3229c|M.tuberculosis_H37Rv ---------------------------------PGATYRSTLTANVVANV
TH_0650|M.thermoresistible__bu ---------------------------------PAASYRSTLTANAMANV
Mvan_1768|M.vanbaalenii_PYR-1 ---------------------------------PRATFRSTLTANAVANV
MAB_3548c|M.abscessus_ATCC_199 ---------------------------------PAGSYRKTVTANLLANV
MMAR_0336|M.marinum_M ---------------------------------PFAPF--VFAGNLTANL
MUL_4785|M.ulcerans_Agy99 ---------------------------------PFAPF--VFAGNLTANL
MAV_5167|M.avium_104 ---------------------------------PFAPF--VFTGNLTANL
Mflv_5246|M.gilvum_PYR-GCK QPSHSRIGKLLRRTTGNRRVGGVATLLDRYASGVESTYLRTLGADALANL
MSMEG_6835|M.smegmatis_MC2_155 RPPHSRLGNRLRRITGNRRVGTVAGLLDRHVAGVESTYLRTLAANALANV
..: .. .: **:
Mb3258c|M.bovis_AF2122/97 IRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGP
Rv3229c|M.tuberculosis_H37Rv IRNVWSNAVIFCGHFPDGAEKFTKTDMIGEPKGQWYLRQMLGSANFNAGP
TH_0650|M.thermoresistible__bu IRNVWSNAVIFCGHFPDGAEKFTKTDMVGESKGQWYLRQMLGSANFEGGW
Mvan_1768|M.vanbaalenii_PYR-1 IRNVWANAVIFCGHFPDGAEKFTKTDMVGESRGQWYLRQMLGSANFEGGP
MAB_3548c|M.abscessus_ATCC_199 IRNIWSNAVIFCGHFPDGAEKFTKQDLENETQGQWYLRQMLGSANFDAGP
MMAR_0336|M.marinum_M MRNVWSYMIIFCGHFPDGTQEFSVEETQNESRGQWYFRQVLGSANLTGGK
MUL_4785|M.ulcerans_Agy99 MRNVWSYMIIFCGHFPDGTQEFSVEETQNESRGQWYFRQVLGSANLTGGK
MAV_5167|M.avium_104 IRNVWSYMIIFCGHFPDGTQEFTVEETQDESRGMWYFRQILGSANLTGGK
Mflv_5246|M.gilvum_PYR-GCK IRNVWAHAIIFCGHFPDQTWTFTEEEVENETRGGWYVRQLLGAANIEGSP
MSMEG_6835|M.smegmatis_MC2_155 IRNVWAHAIIFCGHFPDQTWTFTEEEVENETRGGWYVRQLLGAANIEGSP
:**:*: :******** : *: : .*.:* **.**:**:**: ..
Mb3258c|M.bovis_AF2122/97 ALRFMSGNLCHQIEHHLYPDLPSNRLHEISVRVREVCDRYDLPYTTGSFL
Rv3229c|M.tuberculosis_H37Rv ALRFMSGNLCHQIEHHLYPDLPSNRLHEISVRVREVCDRYDLPYTTGSFL
TH_0650|M.thermoresistible__bu LLRFMSGNLCHQIEHHLYPDLPSNRLHEASIRVREVCDKYDLPYTTGPFL
Mvan_1768|M.vanbaalenii_PYR-1 LLRFMSGNLSQQIEHHLFPDLPSNRYYEISLRVRELCDKYDLPYTTGNFL
MAB_3548c|M.abscessus_ATCC_199 AMAFMSGNLCHQIEHHLFPDLPSNRLSAVSVRVRELCDKYDLPYTSGPFL
MMAR_0336|M.marinum_M TFHLLSGNLSHQIEHHLFPDIPARRYAEIAPEVQEICERYGIPYNRGPLL
MUL_4785|M.ulcerans_Agy99 TFHLLSGNLSHQREHHLFPDIPARRYAEIAPEVQEICELYGIPYNRGPLL
MAV_5167|M.avium_104 LFHLLSGNLSHQIEHHLFPDMPARRYAEIAPEVQAICQRYGVAYNRGPLL
Mflv_5246|M.gilvum_PYR-GCK LFHVLSGNLGYQVEHHLFPDMPSSRYAEIAPKVKDICERYELPYNSGRFG
MSMEG_6835|M.smegmatis_MC2_155 LFHIISGNLGYQVEHHLFPDMPSSRYAEIAPKVKDICERYELPYNSGPFG
: .:**** * ****:**:*: * : .*: :*: * :.*. * :
Mb3258c|M.bovis_AF2122/97 VQYGKTWRTLAKLSLPDKYLR--DNADDAPETRSERMFAGLGPGFAGADP
Rv3229c|M.tuberculosis_H37Rv VQYGKTWRTLAKLSLPDKYLR--DNADDAPETRSERMFAGLGPGFAGADP
TH_0650|M.thermoresistible__bu VQYAKTWRTLAKLSLPDRFLK--ATADNAPETRSERMFAELGPGFAGVDP
Mvan_1768|M.vanbaalenii_PYR-1 VQYGKSWRTIAKLSLPDRFLT--DTADDAPETRSERMFDGLEP-------
MAB_3548c|M.abscessus_ATCC_199 VQYAKAWRTIAKLSLPDKYLT--YTADNAQETRSVKMFDELEP-------
MMAR_0336|M.marinum_M RQFGTVVRKIVKLAFR--------GSDR-QEAHALA--------------
MUL_4785|M.ulcerans_Agy99 RQFGTVVRQIVKLAFR--------GSDR-QEAHALA--------------
MAV_5167|M.avium_104 RQFGTVVRKIVKLSFPNAWI---PGAAKPADREPVAA-------------
Mflv_5246|M.gilvum_PYR-GCK KQWFTVHRTIFRLALPGGTPRPKPGPYRSPERGRSPQGVSEAERFRSRVP
MSMEG_6835|M.smegmatis_MC2_155 HQWFTVQRTIFRLAFPGGTPRPKPGPYRSPDREHAPKGPSEAARFRGRVP
*: . * : :*:: . :
Mb3258c|M.bovis_AF2122/97 VTGRRRGLKTAIAAVRGRRRSKRMAKS-----------------------
Rv3229c|M.tuberculosis_H37Rv VTGRRRGLKTAIAAVRGRRRSKRMAKS-----------------------
TH_0650|M.thermoresistible__bu ATGRRRGLKSAFAAVRNWRRDKQAAKAXXXGELYPRPGTLRRDVGVRLRR
Mvan_1768|M.vanbaalenii_PYR-1 --SERRGLRSAIAAVRKRRRAKAAEKA-----------------------
MAB_3548c|M.abscessus_ATCC_199 --HERRGLKSAFAAVRARRKARHKV-------------------------
MMAR_0336|M.marinum_M --------------------------------------------------
MUL_4785|M.ulcerans_Agy99 --------------------------------------------------
MAV_5167|M.avium_104 --------------------------------------------------
Mflv_5246|M.gilvum_PYR-GCK AEHPAAGPEHESGGVAVAPPARGND-------------------------
MSMEG_6835|M.smegmatis_MC2_155 AEHPDAGPEHASGGVAVQLPPRGND-------------------------
Mb3258c|M.bovis_AF2122/97 --VTEPDDLAA------------------------------------
Rv3229c|M.tuberculosis_H37Rv --VTEPDDLAA------------------------------------
TH_0650|M.thermoresistible__bu GGVPGPAGLWSGEHDRRGHRPPRSGPQRIRRPRGHAVLRRRDRAVRR
Mvan_1768|M.vanbaalenii_PYR-1 -----AVKLAA------------------------------------
MAB_3548c|M.abscessus_ATCC_199 -----------------------------------------------
MMAR_0336|M.marinum_M -----------------------------------------------
MUL_4785|M.ulcerans_Agy99 -----------------------------------------------
MAV_5167|M.avium_104 -----------------------------------------------
Mflv_5246|M.gilvum_PYR-GCK -----------------------------------------------
MSMEG_6835|M.smegmatis_MC2_155 -----------------------------------------------