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PATSGTVLVTGGFGLVGSATVRRLAELGRTVVVADLDTPANRAAAERLPAGVTVRWADLTNAEQTSRLVG EVAPAVIIHLAAIIPPAIYKNRALARRVNVDATATLVKIAEAQPDPPRFVQASSNAVYGARNPHKATGPV TADMPMKHSDLYSALKAEAESIVRASSLEWIVLRLGGVLSVDPKAIPFNADALYFESLLPTDNRVHSVDV RDVAWAFAAATTADAAREILLIAGDESHMLTYADITPALAATRGIKGGLVPGRKGNPDDDDAWFVTDWMD TTRAQEVLQFQHYSWQDMLAEASRNAGPSKYVLPLLSPLIRAALKRRGAYWKQPGQYADPWGAIKRRLGD PAPDA*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2425 | - | - | 100% (356) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_1028 | - | 9e-09 | 30.86% (256) | NAD-dependent epimerase/dehydratase |
| M. gilvum PYR-GCK | Mflv_5007 | - | 2e-07 | 30.06% (173) | dTDP-glucose 4,6-dehydratase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1136c | - | 1e-07 | 31.78% (214) | cholesterol dehydrogenase |
| M. tuberculosis H37Rv | Rv1106c | - | 1e-07 | 31.78% (214) | cholesterol dehydrogenase |
| M. leprae Br4923 | MLBr_01942 | - | 1e-05 | 30.72% (166) | putative cholesterol dehydrogenase |
| M. abscessus ATCC 19977 | MAB_4111c | - | 2e-09 | 32.89% (149) | putative epimerase/dehydratase |
| M. marinum M | MMAR_2336 | galE6 | 1e-06 | 28.66% (164) | UDP-glucose 4-epimerase, GalE6 |
| M. avium 104 | MAV_2497 | - | 2e-65 | 40.61% (362) | RmlD substrate binding domain-containing protein |
| M. smegmatis MC2 155 | MSMEG_5233 | - | 1e-107 | 56.90% (355) | RmlD substrate binding domain-containing protein |
| M. thermoresistible (build 8) | TH_1115 | - | 1e-108 | 56.06% (355) | RmlD substrate binding domain superfamily protein |
| M. ulcerans Agy99 | MUL_0172 | - | 7e-07 | 30.95% (210) | cholesterol dehydrogenase |
| M. vanbaalenii PYR-1 | Mvan_4227 | - | 1e-170 | 81.74% (356) | NAD-dependent epimerase/dehydratase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2425|M.gilvum_PYR-GCK ---------MPATSGTVLVTGGFGLVGSATVRRLAELGRTVVVADLD-TP
Mvan_4227|M.vanbaalenii_PYR-1 ---------MSGTSGTVLVTGGFGLVGSATVRRLVELGRSVVVADLD-TP
MSMEG_5233|M.smegmatis_MC2_155 ------------MSDAVLVTGAFGLVGSAVVATLAAQGRPVVATDVG-TP
TH_1115|M.thermoresistible__bu ------------MSDAVLVTGAFGLVGSATVRRLAADGHRVVATDVA-TP
MAV_2497|M.avium_104 -----------MTANTVLVTGAFGQVGKRCTQLLLQRGRTVVAMDLR-ND
Mb1136c|M.bovis_AF2122/97 MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSL
Rv1106c|M.tuberculosis_H37Rv MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSL
MUL_0172|M.ulcerans_Agy99 MLRPMGDASLTTELGRVLVTGGSGFVGSNLVTTLLDRGHHVRSFDRAPSP
MLBr_01942|M.leprae_Br4923 MLRGVGNASLTTELGRVLVTGGSGFVGANLVTALLERGYQVRSFDRAPMP
MAB_4111c|M.abscessus_ATCC_199 ----------------MLISGGAGFIGSALSNRLIQAGYDVAVMDVLHPQ
MMAR_2336|M.marinum_M --------------MHYLITGHTGFKGPWLALLLLSRGHQVSGLALD-SE
*::* * * * * *
Mflv_2425|M.gilvum_PYR-GCK ANRA-AAERLPAG-----VTVRWADLTNAEQTSRLVGEVAPAVIIHLAAI
Mvan_4227|M.vanbaalenii_PYR-1 ANRA-SAAQLPAG-----VTVRWTDLTDAEQTSALVSEVAPAVIIHLAAI
MSMEG_5233|M.smegmatis_MC2_155 ANRK-SATGLPP-----TVEVRWADLTDAAAVDTLVADVAPAAIVHLAAI
TH_1115|M.thermoresistible__bu DTRK-AAAELSADGPG-EVQVRWADLTDPAAVDELMTTVSPAAVIHLAAV
MAV_2497|M.avium_104 NTAA-VATELAAGGYPGTLIPAYTDLLDAEAVRDLVTAHQPDAVVHLAAV
Mb1136c|M.bovis_AF2122/97 LPAH-PQLEVLQG------DITDADVCAAAVDGIDTIFHTAAIIELMGGA
Rv1106c|M.tuberculosis_H37Rv LPAH-PQLEVLQG------DITDADVCAAAVDGIDTIFHTAAIIELMGGA
MUL_0172|M.ulcerans_Agy99 LPPH-PQLEVLQG------DITDAATCATAVDGVDTVFHTAAIIELMGGA
MLBr_01942|M.leprae_Br4923 LPQH-PQLEVLQG------DITDATVCTTAMDSIDTVFHTAAIIELMGGA
MAB_4111c|M.abscessus_ATCC_199 VHARGRAIDLPAS-----VRLFTGDVTHAPDWDAVLRLFEPSQVVHLAAE
MMAR_2336|M.marinum_M QAGLFKRAGLADQ----LVSDLRVDIRESEATTAALQAVAPDVVIHLAAQ
: . . : :..
Mflv_2425|M.gilvum_PYR-GCK IPPAIYKNRALAR-RVNVDATATLVKIAE-AQPDPPRFVQASSNAVYGAR
Mvan_4227|M.vanbaalenii_PYR-1 IPPAIYKNRALAR-RVNVEATATLVRIAE-AQPTPPRFVQASSNAVYGAR
MSMEG_5233|M.smegmatis_MC2_155 IPPFCYMRRELAR-KVNVDATESLLRAAE-ARATPPRFVLASSVAVYGTR
TH_1115|M.thermoresistible__bu IAPFCYSRQALAR-KVNVDATANLIRAAE-AQSSPPRFIQASSIAVYGPR
MAV_2497|M.avium_104 VSPLSYRKPDLAR-RVNVGGTENLLAACT-ALPRPPLFLMASSAAVYGSR
Mb1136c|M.bovis_AF2122/97 SVTDEYRQRSFA---VNVGGTENLLHAGQ-RAGVQRFVYTSSNSVVMGGQ
Rv1106c|M.tuberculosis_H37Rv SVTDEYRQRSFA---VNVGGTENLLHAGQ-RAGVQRFVYTSSNSVVMGGQ
MUL_0172|M.ulcerans_Agy99 SVTDEYRKRSFS---VNVGGTENLVRAGQ-QAGVKRFVYTSSNSVVMGGQ
MLBr_01942|M.leprae_Br4923 SVTDEYRQRSYT---VNVGGTENLLRAGQ-KSGVKRFVYTASNSVVMGGT
MAB_4111c|M.abscessus_ATCC_199 TGTAQSLSEATRHGSVNVVGTTQLLDALSRSALVPDQLVLASSRAVYGEG
MMAR_2336|M.marinum_M SLVRESYRNPRYTYETNAMGTLNVLEAVG-ATPSVRAHVVITTDKVYRNV
.*. .* :: :. *
Mflv_2425|M.gilvum_PYR-GCK NPHKATGPVTADMPMKHSDLYSALKAEAESIVRA-------SSLEWIVLR
Mvan_4227|M.vanbaalenii_PYR-1 NPYKSAGPVTADMPMKHSDLYSAHKAEAEAIVRA-------SSLEWVVLR
MSMEG_5233|M.smegmatis_MC2_155 NPHRITDMLTADTPVNPVDIYGAHKVEAENLVRA-------SRLDWLILR
TH_1115|M.thermoresistible__bu NPHTCSDVLTADTPPNPGDLYGGHKYEAEQLVRA-------SNLEWAILR
MAV_2497|M.avium_104 NPYRQPERITPDTPVNPIDQYGQDKVLAEAAIRA-------SGLPYALFR
Mb1136c|M.bovis_AF2122/97 NIAGGDETLPYTDRFN--DLYTETKVVAERFVLAQNGV---DGMLTCAIR
Rv1106c|M.tuberculosis_H37Rv NIAGGDETLPYTDRFN--DLYTETKVVAERFVLAQNGV---DGMLTCAIR
MUL_0172|M.ulcerans_Agy99 IIAGGDETLPYTNRFN--DLYTETKVVAEKFVLSQNGN---NEMLTCAIR
MLBr_01942|M.leprae_Br4923 PITGGDETMPYTKRFN--DLYTETKVVAEKFVLSQNGVPDGETMLTCSIR
MAB_4111c|M.abscessus_ATCC_199 AWQSGAEVFYPRPRSHAQLLAGEWDPQGPAGASATPLS---SCADRTEPR
MMAR_2336|M.marinum_M NQEAG---YVETDPLGGDDPYSASKAMADLLTQSWVRS--FPNCPTAIAR
. . : *
Mflv_2425|M.gilvum_PYR-GCK LGGVLS-VDPKAIPFNADALYFESLLPTDN----RVHSVD--VRDVAWAF
Mvan_4227|M.vanbaalenii_PYR-1 LGGVLS-TDPNAIPFSADALYFESVLPADG----RIHTVD--VRDVAWAF
MSMEG_5233|M.smegmatis_MC2_155 LGGVMS-STF-SVDMDVELISLESVLPADG----RLQTVD--VRDVATAF
TH_1115|M.thermoresistible__bu LGGVLS-AEP-MFDMKPEFLYFEALLPTDG----RLQTVE--VRDVAAAF
MAV_2497|M.avium_104 LGGIIS-PDT-HASINGDYLLLMRSMPSDN----RMHAVD--ARDVALAF
Mb1136c|M.bovis_AF2122/97 PSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSY--VHNLIHGF
Rv1106c|M.tuberculosis_H37Rv PSGIWGNGDQTMFRKLFESVLKGHVKVLVGRKSARLDNSY--VHNLIHGF
MUL_0172|M.ulcerans_Agy99 PSGIWGTGDQLMFRKLFESVIKGHVKVLVGPKSVLLDNSY--VDNLIHGF
MLBr_01942|M.leprae_Br4923 PSGIWGRGDQTMFRKAFESVVSGHVKVLIGSKNAKLDNSY--VHNLVHGL
MAB_4111c|M.abscessus_ATCC_199 PTNIYAGTKLAQEHLLASWAAAHDTNLSVL----RLQNVYGPGQSLTNSY
MMAR_2336|M.marinum_M AGNVIGGGDISRDRLFPDLVDAYARGQAPRLRFPRAVRPWQHVLDCLNGY
.: . . . .
Mflv_2425|M.gilvum_PYR-GCK AAATTADAAR---EILLIAGD-ESHMLTYADITPALAATRGI-KGGLVPG
Mvan_4227|M.vanbaalenii_PYR-1 AAATTADVAR---EILLIAGD-DSHRVLQGDVGRALAESRGL-KGGLVPG
MSMEG_5233|M.smegmatis_MC2_155 AAATTVPVETANHEVLLIGGDPQTHRHTQARVGSEAAQAMGI-RGGLPAG
TH_1115|M.thermoresistible__bu TAAVTAPVAG---QVLLIGGD-DSHRLTQGEIGPSIAAAMGL-VGALPVG
MAV_2497|M.avium_104 ANGVDRAATISG-KVLLIGGN-ESYVLTQRELEDAMMEAVGLGRLGPSAS
Mb1136c|M.bovis_AF2122/97 ILAAAHLVPDGTAPGQAYFIN-DAEPINMFEFARPVLEACGQRWPKMRIS
Rv1106c|M.tuberculosis_H37Rv ILAAAHLVPDGTAPGQAYFIN-DAEPINMFEFARPVLEACGQRWPKMRIS
MUL_0172|M.ulcerans_Agy99 MLAAQHLVPGGSAPGQAYFIN-DAEPINMFDFARPVVEACGEKWPRVRVS
MLBr_01942|M.leprae_Br4923 ILAAEHLVPGGTAPGQAYFIN-DGEPINFFDFMGPIIKACGENWPRVRIS
MAB_4111c|M.abscessus_ATCC_199 TGIVTLFARLAREKQALEVYE-DGRIVRDFVFIDDVVDALFAAVHTPATE
MMAR_2336|M.marinum_M LTLADALLAGSGLGQWNFGPG-RDSFVEVGQVASLAAELWGGGAHWDLDD
. .
Mflv_2425|M.gilvum_PYR-GCK RKGNPDDDDAWFVTDWMD--TTRAQEVLQFQHYSWQDMLAEASRNAGPSK
Mvan_4227|M.vanbaalenii_PYR-1 RNGDPNSDENWFVTDWMD--TRRAQEALQFQHYSWQNMLDEAQRRAGASR
MSMEG_5233|M.smegmatis_MC2_155 RPGNPDDDHAWFHTDWMD--TTRAQEVLTFQHHSWPQLLADTRANAGWKR
TH_1115|M.thermoresistible__bu RKGNPHSDTDWFATDWLD--PGPAQELLKFQHYSWPDILAETADRVGWKR
MAV_2497|M.avium_104 LPGNPADDRGWSFTGWYD--TTEAQQLLDFQEHDWSQTVAWLAESQGRSR
Mb1136c|M.bovis_AF2122/97 GPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGY
Rv1106c|M.tuberculosis_H37Rv GPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGY
MUL_0172|M.ulcerans_Agy99 GPMVHRAMTGWQRLHFRFGLPAPLLEPLAVERLYLDNYFSVDKARRDLGY
MLBr_01942|M.leprae_Br4923 GRLVRNVMAVWQRLHFGFGLPKPPMEPLAVERVYLDNYFSIEKAHKELGY
MAB_4111c|M.abscessus_ATCC_199 R---RTLDIGSGTATTIHELARKVADICAAPEPVVVGKFRDGDVRAASCD
MMAR_2336|M.marinum_M GDHPHEAN------------LLALDAAKAQAELGWRNRLGFRDAVAWTID
. .
Mflv_2425|M.gilvum_PYR-GCK YVLPLLSPLIRAALKRRGAYWKQP---GQYADPWGAIKRRLGDPAPDA--
Mvan_4227|M.vanbaalenii_PYR-1 YVLPVFAPLVRAVLKRRSAYWKQP---GQYADPWGAIKRGIGDPSPDS--
MSMEG_5233|M.smegmatis_MC2_155 YPLGLAAPLIRAFLRSKSAYKNYP---GRYADVWNAVEKRWGNPRPDRSE
TH_1115|M.thermoresistible__bu YPLRLIAPLAGLYLRRQSPYHNHP---GQYADPWGAIRRKWGEPGLDTDP
MAV_2497|M.avium_104 LALRLLGPLLRPLLRTILIVQRRLEGRGPYADPWTLIEKKYGTAALATAD
Mb1136c|M.bovis_AF2122/97 EPLFTTQQALTECLP-------------YYVSLFEQMKNEARAEKTAATV
Rv1106c|M.tuberculosis_H37Rv EPLFTTQQALTECLP-------------YYVSLFEQMKNEARAEKTAATV
MUL_0172|M.ulcerans_Agy99 EPKFTTEQALKECLP-------------YYVDLFRQMKSEVKAGGR----
MLBr_01942|M.leprae_Br4923 RPLFTTEQAMAECLP-------------YYTELFEQIKVAAKPHLASIAA
MAB_4111c|M.abscessus_ATCC_199 IAPAVEGLGWSPKWT--------------LEDGLHALLEWIDKDCPNRF-
MMAR_2336|M.marinum_M WERQVHG----------------------GADPLAVTRDQIAAFESLE--
.
Mflv_2425|M.gilvum_PYR-GCK -----
Mvan_4227|M.vanbaalenii_PYR-1 -----
MSMEG_5233|M.smegmatis_MC2_155 A----
TH_1115|M.thermoresistible__bu EDGR-
MAV_2497|M.avium_104 -----
Mb1136c|M.bovis_AF2122/97 KP---
Rv1106c|M.tuberculosis_H37Rv KP---
MUL_0172|M.ulcerans_Agy99 -----
MLBr_01942|M.leprae_Br4923 TPRPE
MAB_4111c|M.abscessus_ATCC_199 -----
MMAR_2336|M.marinum_M -----