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VWSCRRWAPTDHGPPPTTPPRWGGSTFHSEWELAAAQRHWDSAGHLLPFQPASFRDFMLYERHAVDAARG MVGRFMPAAAPVGAAFEKASGRTFAPFKPKPLFYRQPIYYMSNHLSFVPSGTPVAFPSYTEALDYELELG FVLRAPLRNATPREALDAIGAFVVVNDFSARDVQRAEMVTGLGPQKAKHFCSSMSEVAVTAGEVLPVAER LTGSVSINGTTVSMVSSAGPQWSIGDMLAHASRDETLYAGELFAAGTLPGGSGMETGHWLRPGDHLVLTI DGIGEVSHQIVP
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_2168 | - | - | 100% (292) | fumarylacetoacetate (FAA) hydrolase |
| M. gilvum PYR-GCK | Mflv_4230 | - | 2e-09 | 25.13% (191) | 5-carboxymethyl-2-hydroxymuconate Delta-isomerase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0964 | - | 2e-17 | 30.95% (168) | bifunctional 2-hydroxyhepta-2,4-diene-1,7-dioate |
| M. tuberculosis H37Rv | Rv0939 | - | 2e-17 | 30.95% (168) | bifunctional 2-hydroxyhepta-2,4-diene-1,7-dioate |
| M. leprae Br4923 | MLBr_01689 | - | 1e-10 | 25.13% (187) | putative hydrolase |
| M. abscessus ATCC 19977 | MAB_4361 | - | 2e-12 | 29.27% (164) | fumarylacetoacetate hydrolase family protein |
| M. marinum M | MMAR_4572 | - | 5e-15 | 25.87% (201) | bifunctional enzyme: 2-hydroxyhepta-2,4-diene- 1,7-dioate |
| M. avium 104 | MAV_1057 | - | 2e-17 | 26.37% (201) | fumarylacetoacetate hydrolase family protein, putative |
| M. smegmatis MC2 155 | MSMEG_6588 | - | 1e-102 | 64.44% (284) | fumarylacetoacetate |
| M. thermoresistible (build 8) | TH_0259 | - | 4e-18 | 28.43% (204) | POSSIBLE BIFUNCTIONAL ENZYME: |
| M. ulcerans Agy99 | MUL_1956 | - | 5e-10 | 24.47% (188) | 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase |
| M. vanbaalenii PYR-1 | Mvan_4532 | - | 1e-117 | 73.05% (282) | fumarylacetoacetate (FAA) hydrolase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0964|M.bovis_AF2122/97 -------MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLR
Rv0939|M.tuberculosis_H37Rv -------MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLR
MMAR_4572|M.marinum_M -------MKWVTYRSADGERVGVLSDGSIYAMAPGVALLDLIKRGADGLR
MAV_1057|M.avium_104 -------MKWVTYQGDDGERVGVLRDDTIYAMGSGVTLLDLIARGAEGLR
TH_0259|M.thermoresistible__bu -------------------------------MPPGTELIDLVALGADGLR
MLBr_01689|M.leprae_Br4923 -----------------------------------MFARELAEYPFGELT
MUL_1956|M.ulcerans_Agy99 -----------------------------------MTAREIAEDPFGTPN
MAB_4361|M.abscessus_ATCC_1997 -----------------MRLYTTDRGIGREDRHGILALLDLPHRDLGELL
Mflv_2168|M.gilvum_PYR-GCK -----MWSCRRWAPTDHGP-----PPTTPPRWGGSTFHSEWELAAAQRHW
Mvan_4532|M.vanbaalenii_PYR-1 MKLRRVIADGRLELQSLGPDGSWAPAPDPSVLGGPVFDPAWELAAAQRHH
MSMEG_6588|M.smegmatis_MC2_155 MKLRRVRTAADLRVESLDADGNWVPHDDVAPLGGRVFDAEWLAAAADRQL
Mb0964|M.bovis_AF2122/97 TAG---ERAVRSPAAVVALDEVTLAAPIPRP-PSIRDSLCFLDHMRNCQE
Rv0939|M.tuberculosis_H37Rv TAG---ERAVRSPAAVVALDEVTLAAPIPRP-PSIRDSLCFLDHMRNCQE
MMAR_4572|M.marinum_M EAG---ENVLRSPSEVVALDEVTLAAPIPRP-PSIRDSLCFLDHMRNCQE
MAV_1057|M.avium_104 HAG---EEAQRSPAATVPAARARLLAPIPRP-PSIRDSLCFLDHMRNCQA
TH_0259|M.thermoresistible__bu DAG---ERALRDPSEVVPLSDVVLRAPIPRP-PAIRDCLCFLDHMRNCQE
MLBr_01689|M.leprae_Br4923 FTG-----------RSWPLADVRLLAPMLAS-KVVCIGKNYADH------
MUL_1956|M.ulcerans_Agy99 FTG-----------RSWPLADVRLLAPILAS-KVVCVGKNYTGH------
MAB_4361|M.abscessus_ATCC_1997 SDTGLDPARDATVTDEVALTDVAVLAPVIRPGKILIIGLNYPSHAKEALA
Mflv_2168|M.gilvum_PYR-GCK DSAGHLLPFQPASFRDFMLYERHAVDAARGMVGRFMPAAAPVGAAFEKAS
Mvan_4532|M.vanbaalenii_PYR-1 GLAGCVLPFQPASFRDFMLYEDHAIDAARGLVRRFHPAQSRAAETVEKLT
MSMEG_6588|M.smegmatis_MC2_155 QHSPHLLPFQPVSFRDFMLYEQHNIDAARGLIRRFHPGLYRATSAFERLT
. .
Mb0964|M.bovis_AF2122/97 AMGGGRVLMDTWYRIPAFYFACPSTVLGP-YDDAPTAPGSAWQDFELEIA
Rv0939|M.tuberculosis_H37Rv AMGGGRVLMDTWYRIPAFYFACPSTVLGP-YDDAPTAPGSAWQDFELEIA
MMAR_4572|M.marinum_M AVGGGRVLMDTWYRIPAFYFACPATVLGP-YDDAPMAPGSAWQDFELEIA
MAV_1057|M.avium_104 ALGAGRVLADTWYRVPAFYFACPATVLGP-YDDAPTAPGSAWQDFELEIA
TH_0259|M.thermoresistible__bu ALGGGRVLKDAWYRIPAFYFANPSAVFGP-YDDVPTAPGSAWQDFELEIA
MLBr_01689|M.leprae_Br4923 -IVEMGGQTGLASADPVIFLKPNTAIIGPNVPIRLPANAS-PVHFEGELA
MUL_1956|M.ulcerans_Agy99 -IAEMGGQTGPTPADPVIFLKSNTAIVGPNVPIRLPANAS-PVHFEGELA
MAB_4361|M.abscessus_ATCC_1997 AFAAMGRDDIVMPTEPNMQLIAGSATTGTDHTIKLPAIAPDEVDYEGELA
Mflv_2168|M.gilvum_PYR-GCK GRTFAPFKPKPLFYRQPIYYMSNHLSFVPSGTPVAFPSYTEALDYELELG
Mvan_4532|M.vanbaalenii_PYR-1 RKPFPLFKPKPLFYRQPIYYMSNHLSFVPSGAPVAFPSYSTALDFELELG
MSMEG_6588|M.smegmatis_MC2_155 GRPVPQFKPKPLFYRQPIYYMSNAQTFVPTGTPVPVPDYSQALDFELEIG
: . . . .:* *:.
Mb0964|M.bovis_AF2122/97 AVIGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLEG----QLRIGQAK
Rv0939|M.tuberculosis_H37Rv AVIGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLEG----QLRIGQAK
MMAR_4572|M.marinum_M AVIGTGGQDLSVEQAEQSIIGYTIFNDWSARDLQQLEG----QLRIGQAK
MAV_1057|M.avium_104 AVIGTGGKDLSVEQAEQAIIGYTIFNDWSARDLQQLDS----QLGIGQAK
TH_0259|M.thermoresistible__bu AVIGTGGADLTVAEAEQAIIGYTIFNDWSARDLQSLES----QLGIGQAK
MLBr_01689|M.leprae_Br4923 LVVGRSCKDVPAVQAVDCILGYTIGNDMSARDQQR------ADGQWTRAK
MUL_1956|M.ulcerans_Agy99 IVIGRPCKDVSAAQAVDNILGYTIGNDVSARDQQK------SDGQWTRAK
MAB_4361|M.abscessus_ATCC_1997 VVIGRSTHAVSVEEAWKHVAGLTIANDVGARDIQRRAMTGDAAASIGVAK
Mflv_2168|M.gilvum_PYR-GCK FVLRAPLRNATPREALDAIGAFVVVNDFSARDVQRAEM----VTGLGPQK
Mvan_4532|M.vanbaalenii_PYR-1 FVLKAPLFNASSAEAVDAVGAFVVVNDFSARDVQRAEM----ATGLGPQK
MSMEG_6588|M.smegmatis_MC2_155 FVLAAPLFNATPNEATAAIGAFVVLNDFSARDVQRAEM----TSGFGPQK
*: . :* : . .: ** .*** * *
Mb0964|M.bovis_AF2122/97 GKDSGITLGPYLVTPDELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDW
Rv0939|M.tuberculosis_H37Rv GKDSGITLGPYLVTPDELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDW
MMAR_4572|M.marinum_M GKDSGVTLGPYLVTADELQAYRRDGKLSLQVSALVNDTVIGSGSTATMDW
MAV_1057|M.avium_104 GKDSGVTLGPYLVTPDELEPYRRNGKLDLRVTASVNDTVIGSGSTAQMDW
TH_0259|M.thermoresistible__bu GKDSGITLGPYLVTPDELDEFRTDGRLDLTVTALVNGEVIGSGSTAAMDW
MLBr_01689|M.leprae_Br4923 GHDTFCPVGPWIVTDLGPL-----DPADLELRTAVNGEVKQHSRTSLMIH
MUL_1956|M.ulcerans_Agy99 GHDTFCPVGPWIVTDV--------DPAGLELRTEVNGAVKQHARTSLMIH
MAB_4361|M.abscessus_ATCC_1997 SFDTFKPLGPCLVTADEFS-----DGVDLTLQTYVNGELRQDDRTGSFIY
Mflv_2168|M.gilvum_PYR-GCK AKHFCSSMSEVAVTAGEVLP----VAERLTGSVSINGTTVSMVSSAGPQW
Mvan_4532|M.vanbaalenii_PYR-1 AKHFVSSMSAVAVTADEILP----ILESLSGSVTINGRTVSTVSSAGPQW
MSMEG_6588|M.smegmatis_MC2_155 SKHFASSMSDTAVTADEVLP----RIDALTGSVAVNGEIVSTVDSAGMQH
. . .:. ** * . :*. :.
Mb0964|M.bovis_AF2122/97 SFGEVIAYASRGVTLTPGDVFGSGTVPTCTLVEHLRPP--ESFPGWLHDG
Rv0939|M.tuberculosis_H37Rv SFGEVIAYASRGVTLTPGDVFGSGTVPTCTLVEHLRPP--ESFPGWLHDG
MMAR_4572|M.marinum_M TFGEVISYVSRGVMLAPGDVIGSGTVPTCTLVEHLTNP--DSFPGWLRDG
MAV_1057|M.avium_104 TFGEVISYASRGVTLVPGDVIGSGTVPTCTLVEHLDPAALESFPGWLHDG
TH_0259|M.thermoresistible__bu TFAEVISYASRGVFLHPGEVFGSGTVPTCTLVEHLDMTDLAGFRGWLSDG
MLBr_01689|M.leprae_Br4923 GIGAIMEWISAVMTLLPGDLILTGTPAGVGPVE---------------DG
MUL_1956|M.ulcerans_Agy99 DVGAIVEWISAVMTLLPGDLILTGTPAGVGPIE---------------DG
MAB_4361|M.abscessus_ATCC_1997 PIPELIAHLSRYQTLQPGDVICTGTPCGAGAFTGRFLR----------SG
Mflv_2168|M.gilvum_PYR-GCK SIGDMLAHASRDETLYAGELFAAGTLPGGSGMETGHWLR---------PG
Mvan_4532|M.vanbaalenii_PYR-1 TIGDMLAHASRDEKLYPGELFATGTLPGGSGMETGHWLR---------PG
MSMEG_6588|M.smegmatis_MC2_155 SLGEVLAHASRCEPLYPGELFGTGTLPGGSGMETGHWLR---------PG
. :: * * .*::: :** . *
Mb0964|M.bovis_AF2122/97 DVVTLQVEGLGETRQTVRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPF
Rv0939|M.tuberculosis_H37Rv DVVTLQVEGLGETRQTVRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPF
MMAR_4572|M.marinum_M DVVTLEVEGLGQTRQRVCATPPPQPLAPRVDPNAAPEAARVNPAPPLVPY
MAV_1057|M.avium_104 DVVTLRVEGLGETRQTVRASGAPHPLAARPNPDAPPAAPRVNRAAARVPY
TH_0259|M.thermoresistible__bu DEVTLQVQGLGETRQTVRHRPAPTLLPPRPNPDAAPAPARVNPAPAKVPY
MLBr_01689|M.leprae_Br4923 DIVSITVEGIGTLTNPVVRKGKS---------------------------
MUL_1956|M.ulcerans_Agy99 DTVSITIEGIDTLTNPVVRKGKS---------------------------
MAB_4361|M.abscessus_ATCC_1997 DTVEVAIERIGLLRNRVSAA------------------------------
Mflv_2168|M.gilvum_PYR-GCK DHLVLTIDGIGEVSHQIVP-------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 DRITLSIEQVGEISHQITS-------------------------------
MSMEG_6588|M.smegmatis_MC2_155 DTLTLILDDIGRIEHTIG--------------------------------
* : : :: :. : :
Mb0964|M.bovis_AF2122/97 TRGLHEVADRVWAWTLPDGGYGFSNAGLVAGDGASLLVDTLFDLALTREM
Rv0939|M.tuberculosis_H37Rv TRGLHEVADRVWAWTLPDGGYGFSNAGLVAGDGASLLVDTLFDLALTREM
MMAR_4572|M.marinum_M TRGLHQVADRVWAWTLPDGGFGWSNAGLVAGDGASLLVDTLFDLALTREM
MAV_1057|M.avium_104 TRGLHQVGDRVWAWTLPDGGYGWSNAGLIAGDGASLLVDTLFDLALTREM
TH_0259|M.thermoresistible__bu RRGLHQVGENVWAWTLPDGGYGWSNAGLVAGEGASLLVDTLFDLTLTREM
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 LAAMKPVTERAPITDALITHSNGDHTHGTQLLDRSVRIIAAKGTSEEIEH
Rv0939|M.tuberculosis_H37Rv LAAMKPVTERAPITDALITHSNGDHTHGTQLLDRSVRIIAAKGTSEEIEH
MMAR_4572|M.marinum_M LDAMRPITDAAPITDALITHSNGDHTHGNQLLSPSVRIIAAKGTAEEIAE
MAV_1057|M.avium_104 LTAMRPITASAPITDAVITHSNGDHTHGNQLLDPAVRIIAAHGTAEEIAH
TH_0259|M.thermoresistible__bu LDAMQSITQRAPITDMVITHCNGDHTHGNQLLDPSVRIIAAKETKDEIDH
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 GPAPEMLARIQTADLGPVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAI
Rv0939|M.tuberculosis_H37Rv GPAPEMLARIQTADLGPVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAI
MMAR_4572|M.marinum_M DTGPTLLTAMQTIDLGPVATRFMRDRFGHFDFSGIRLRNADQTFDHELTI
MAV_1057|M.avium_104 GMAPEMLAMAQTANLGPVATRYTRERFGHFDFSNITLRNADETFDRSLAV
TH_0259|M.thermoresistible__bu EMAPSMLALAQTGDLGPIATTYARDRFGHFDFSGITIRNADHTFDKRLDL
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 ELGGRRVDLLNLGPAHTTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPI
Rv0939|M.tuberculosis_H37Rv ELGGRRVDLLNLGPAHTTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPI
MMAR_4572|M.marinum_M DVGGRRVDLINLGPAHTAADSVVHVPDAGVLFAGDLLFIGCTPIVWNGPI
MAV_1057|M.avium_104 EVGGRRVDVLNLGPAHTAADSVVHVPDAGVLFGGDLLFIGCTPIVWAGPI
TH_0259|M.thermoresistible__bu EVGGRQVTLLNLGPAHTAADTVIHIPDAGVLFGGDLLFIGCTPIVWGGPI
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 ANWVAACDAMIALDAPTVVPGHGPVTGPDGIRAVRGYLAHIAEQAEAAYR
Rv0939|M.tuberculosis_H37Rv ANWVAACDAMIALDAPTVVPGHGPVTGPDGIRAVRGYLAHIAEQAEAAYR
MMAR_4572|M.marinum_M ANWIAACDAMIALDAPIVVPGHGPVTDPDGIRAVRGYFEHVNEQAEAAYR
MAV_1057|M.avium_104 ANWVAACDAMIALDAPTVVPGHGPVTDPDGIRAVRGYLVHIAEQAEAAYR
TH_0259|M.thermoresistible__bu ANWIAACDTMLALDAPTVVPGHGPITGPEGIHAVRDYFEYITEQADDAHR
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 KGLSLPEAVETIDLGEYASWLDSERVVVNVYQRYRELDPDTPRQDLLALL
Rv0939|M.tuberculosis_H37Rv KGLSLPEAVETIDLGEYASWLDSERVVVNVYQRYRELDPDTPRQDLLALL
MMAR_4572|M.marinum_M KGLSLAEAIETVELGDYAAWLDSERIVVNIYQRYRELDPHTPEVERLALL
MAV_1057|M.avium_104 KGLSWPEAADTIDLGEYATWLDAERVVVNVYQRYRELDPHTPQLEVMALL
TH_0259|M.thermoresistible__bu RGLSFIEAADTIDLGPYANWLDAERVVVNVYQRYRELDPDTPQLGVITLL
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MAB_4361|M.abscessus_ATCC_1997 --------------------------------------------------
Mflv_2168|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_4532|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_6588|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 VMQAEWAARHCT-
Rv0939|M.tuberculosis_H37Rv VMQAEWAARHCT-
MMAR_4572|M.marinum_M VMQAEWAAKRV--
MAV_1057|M.avium_104 VMQAEWLAKRS--
TH_0259|M.thermoresistible__bu TMQAEWHAKRGAR
MLBr_01689|M.leprae_Br4923 -------------
MUL_1956|M.ulcerans_Agy99 -------------
MAB_4361|M.abscessus_ATCC_1997 -------------
Mflv_2168|M.gilvum_PYR-GCK -------------
Mvan_4532|M.vanbaalenii_PYR-1 -------------
MSMEG_6588|M.smegmatis_MC2_155 -------------