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MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLRTAGERAVRSPAAVVALDEVTLAAPIPR PPSIRDSLCFLDHMRNCQEAMGGGRVLMDTWYRIPAFYFACPSTVLGPYDDAPTAPGSAWQDFELEIAAV IGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLEGQLRIGQAKGKDSGITLGPYLVTPDELEPYCRGGK LSLRVIALVNGTVIGSGSTAQMDWSFGEVIAYASRGVTLTPGDVFGSGTVPTCTLVEHLRPPESFPGWLH DGDVVTLQVEGLGETRQTVRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPFTRGLHEVADRVWAWTLPD GGYGFSNAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAPITDALITHSNGDHTHGTQLLDRSVRI IAAKGTSEEIEHGPAPEMLARIQTADLGPVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAIELGGRRVD LLNLGPAHTTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAPTVVPGHGPVTGP DGIRAVRGYLAHIAEQAEAAYRKGLSLPEAVETIDLGEYASWLDSERVVVNVYQRYRELDPDTPRQDLLA LLVMQAEWAARHCT
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0964 | - | - | 100% (644) | bifunctional 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase/cyclase/dehydrase |
| M. bovis AF2122 / 97 | Mb3017c | - | 7e-21 | 31.58% (247) | 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase |
| M. bovis AF2122 / 97 | Mb1948 | - | 2e-12 | 28.63% (227) | hypothetical protein Mb1948 |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_4230 | - | 2e-20 | 32.24% (245) | 5-carboxymethyl-2-hydroxymuconate Delta-isomerase |
| M. tuberculosis H37Rv | Rv0939 | - | 0.0 | 100.00% (644) | bifunctional 2-hydroxyhepta-2,4-diene-1,7-dioate |
| M. leprae Br4923 | MLBr_01689 | - | 6e-19 | 31.02% (245) | putative hydrolase |
| M. abscessus ATCC 19977 | MAB_2020c | - | 2e-40 | 33.33% (306) | bifunctional HHDD isomerase/cyclase/dehydrase |
| M. marinum M | MMAR_4572 | - | 0.0 | 80.81% (641) | bifunctional enzyme: 2-hydroxyhepta-2,4-diene- 1,7-dioate |
| M. avium 104 | MAV_1057 | - | 0.0 | 81.96% (643) | fumarylacetoacetate hydrolase family protein, putative |
| M. smegmatis MC2 155 | MSMEG_2478 | - | 1e-21 | 32.53% (249) | fumarylacetoacetate hydrolase family protein |
| M. thermoresistible (build 8) | TH_0259 | - | 0.0 | 74.47% (619) | POSSIBLE BIFUNCTIONAL ENZYME: |
| M. ulcerans Agy99 | MUL_1956 | - | 4e-17 | 31.16% (199) | 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase |
| M. vanbaalenii PYR-1 | Mvan_2132 | - | 7e-20 | 32.65% (245) | 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase |
CLUSTAL 2.0.9 multiple sequence alignment
Mb0964|M.bovis_AF2122/97 MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLRTAGERAV
Rv0939|M.tuberculosis_H37Rv MKWVTYRSDHGERTGVLSGDAIYAMPPDVSLLDLVGRGADGLRTAGERAV
MMAR_4572|M.marinum_M MKWVTYRSADGERVGVLSDGSIYAMAPGVALLDLIKRGADGLREAGENVL
MAV_1057|M.avium_104 MKWVTYQGDDGERVGVLRDDTIYAMGSGVTLLDLIARGAEGLRHAGEEAQ
TH_0259|M.thermoresistible__bu ------------------------MPPGTELIDLVALGADGLRDAGERAL
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 RSPAAVVALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAMGGGRVLMDT
Rv0939|M.tuberculosis_H37Rv RSPAAVVALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAMGGGRVLMDT
MMAR_4572|M.marinum_M RSPSEVVALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAVGGGRVLMDT
MAV_1057|M.avium_104 RSPAATVPAARARLLAPIPRPPSIRDSLCFLDHMRNCQAALGAGRVLADT
TH_0259|M.thermoresistible__bu RDPSEVVPLSDVVLRAPIPRPPAIRDCLCFLDHMRNCQEALGGGRVLKDA
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 WYRIPAFYFACPSTVLGPYDDAPTAPGSAWQDFELEIAAVIGTSGKDLTV
Rv0939|M.tuberculosis_H37Rv WYRIPAFYFACPSTVLGPYDDAPTAPGSAWQDFELEIAAVIGTSGKDLTV
MMAR_4572|M.marinum_M WYRIPAFYFACPATVLGPYDDAPMAPGSAWQDFELEIAAVIGTGGQDLSV
MAV_1057|M.avium_104 WYRVPAFYFACPATVLGPYDDAPTAPGSAWQDFELEIAAVIGTGGKDLSV
TH_0259|M.thermoresistible__bu WYRIPAFYFANPSAVFGPYDDVPTAPGSAWQDFELEIAAVIGTGGADLTV
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 EQAERSIIGYTIFNDWSARDLQMLEGQLRIGQAKGKDSGITLGPYLVTPD
Rv0939|M.tuberculosis_H37Rv EQAERSIIGYTIFNDWSARDLQMLEGQLRIGQAKGKDSGITLGPYLVTPD
MMAR_4572|M.marinum_M EQAEQSIIGYTIFNDWSARDLQQLEGQLRIGQAKGKDSGVTLGPYLVTAD
MAV_1057|M.avium_104 EQAEQAIIGYTIFNDWSARDLQQLDSQLGIGQAKGKDSGVTLGPYLVTPD
TH_0259|M.thermoresistible__bu AEAEQAIIGYTIFNDWSARDLQSLESQLGIGQAKGKDSGITLGPYLVTPD
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 ELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDWSFGEVIAYASRGVTLT
Rv0939|M.tuberculosis_H37Rv ELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDWSFGEVIAYASRGVTLT
MMAR_4572|M.marinum_M ELQAYRRDGKLSLQVSALVNDTVIGSGSTATMDWTFGEVISYVSRGVMLA
MAV_1057|M.avium_104 ELEPYRRNGKLDLRVTASVNDTVIGSGSTAQMDWTFGEVISYASRGVTLV
TH_0259|M.thermoresistible__bu ELDEFRTDGRLDLTVTALVNGEVIGSGSTAAMDWTFAEVISYASRGVFLH
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 PGDVFGSGTVPTCTLVEHLRPP--ESFPGWLHDGDVVTLQVEGLGETRQT
Rv0939|M.tuberculosis_H37Rv PGDVFGSGTVPTCTLVEHLRPP--ESFPGWLHDGDVVTLQVEGLGETRQT
MMAR_4572|M.marinum_M PGDVIGSGTVPTCTLVEHLTNP--DSFPGWLRDGDVVTLEVEGLGQTRQR
MAV_1057|M.avium_104 PGDVIGSGTVPTCTLVEHLDPAALESFPGWLHDGDVVTLRVEGLGETRQT
TH_0259|M.thermoresistible__bu PGEVFGSGTVPTCTLVEHLDMTDLAGFRGWLSDGDEVTLQVQGLGETRQT
MAB_2020c|M.abscessus_ATCC_199 --------------------------------------------------
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 VRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPFTRGLHEVADRVWAWTL
Rv0939|M.tuberculosis_H37Rv VRTSGTPFPLALRPNPDAEPDRRGVNPAPTRVPFTRGLHEVADRVWAWTL
MMAR_4572|M.marinum_M VCATPPPQPLAPRVDPNAAPEAARVNPAPPLVPYTRGLHQVADRVWAWTL
MAV_1057|M.avium_104 VRASGAPHPLAARPNPDAPPAAPRVNRAAARVPYTRGLHQVGDRVWAWTL
TH_0259|M.thermoresistible__bu VRHRPAPTLLPPRPNPDAAPAPARVNPAPAKVPYRRGLHQVGENVWAWTL
MAB_2020c|M.abscessus_ATCC_199 ---------------------------MAQDFAFQKGLVPLGGGAHVFLS
Mflv_4230|M.gilvum_PYR-GCK --------------------------------------------------
Mvan_2132|M.vanbaalenii_PYR-1 --------------------------------------------------
MLBr_01689|M.leprae_Br4923 --------------------------------------------------
MUL_1956|M.ulcerans_Agy99 --------------------------------------------------
MSMEG_2478|M.smegmatis_MC2_155 --------------------------------------------------
Mb0964|M.bovis_AF2122/97 PDGGYGFSNAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAPITDA
Rv0939|M.tuberculosis_H37Rv PDGGYGFSNAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAPITDA
MMAR_4572|M.marinum_M PDGGFGWSNAGLVAGDGASLLVDTLFDLALTREMLDAMRPITDAAPITDA
MAV_1057|M.avium_104 PDGGYGWSNAGLIAGDGASLLVDTLFDLALTREMLTAMRPITASAPITDA
TH_0259|M.thermoresistible__bu PDGGYGWSNAGLVAGEGASLLVDTLFDLTLTREMLDAMQSITQRAPITDM
MAB_2020c|M.abscessus_ATCC_199 GDEGFGLANAGLVVSDDESLVVDTLYDVRHAREMLDEFGRHTATAPVRYV
Mflv_4230|M.gilvum_PYR-GCK ------MRLGRIASPDGVAFVAVEGPPDDPAAAVAREIAEHPFG-----T
Mvan_2132|M.vanbaalenii_PYR-1 ------MRLGRIASPDGVAFVAIEGPPDDPGGAVAREIAEHPFG-----N
MLBr_01689|M.leprae_Br4923 --------------------------------MFARELAEYPFG-----E
MUL_1956|M.ulcerans_Agy99 --------------------------------MTAREIAEDPFG-----T
MSMEG_2478|M.smegmatis_MC2_155 ------MRVVGIRRTGSDRVEVAALTDTGDAVRVLAGLDEFWCDPTAHLA
:
Mb0964|M.bovis_AF2122/97 LITHSNGDHTHGTQLLDRSVRIIAAKGTSEEIEHGPAPEMLARIQTADLG
Rv0939|M.tuberculosis_H37Rv LITHSNGDHTHGTQLLDRSVRIIAAKGTSEEIEHGPAPEMLARIQTADLG
MMAR_4572|M.marinum_M LITHSNGDHTHGNQLLSPSVRIIAAKGTAEEIAEDTGPTLLTAMQTIDLG
MAV_1057|M.avium_104 VITHSNGDHTHGNQLLDPAVRIIAAHGTAEEIAHGMAPEMLAMAQTANLG
TH_0259|M.thermoresistible__bu VITHCNGDHTHGNQLLDPSVRIIAAKETKDEIDHEMAPSMLALAQTGDLG
MAB_2020c|M.abscessus_ATCC_199 FNTHTDGDHFFGNQVFADDVEIITTEAARALMVQEHADLTAELLGGSSDP
Mflv_4230|M.gilvum_PYR-GCK PTFTGRQWPLADVRLLAPILA-SKVVCMGKNYAAHIEEMGGGT---FEDP
Mvan_2132|M.vanbaalenii_PYR-1 PTFTGRQWPLADVRLLAPILA-SKVVCMGKNYAAHIEEMGGGT---FEDP
MLBr_01689|M.leprae_Br4923 LTFTGRSWPLADVRLLAPMLA-SKVVCIGKNYADHIVEMGGQTGLASADP
MUL_1956|M.ulcerans_Agy99 PNFTGRSWPLADVRLLAPILA-SKVVCVGKNYTGHIAEMGGQTGPTPADP
MSMEG_2478|M.smegmatis_MC2_155 EEPAGAVLPRDEVTLVPPVLPGAKVICIGLNYLKHVAEGSYGAEPLPEFP
:. : .
Mb0964|M.bovis_AF2122/97 PVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAIELGGRRVDLLNLGPAH
Rv0939|M.tuberculosis_H37Rv PVATRYLRDRFGHFDFSGIKLRNADLTFDRDLAIELGGRRVDLLNLGPAH
MMAR_4572|M.marinum_M PVATRFMRDRFGHFDFSGIRLRNADQTFDHELTIDVGGRRVDLINLGPAH
MAV_1057|M.avium_104 PVATRYTRERFGHFDFSNITLRNADETFDRSLAVEVGGRRVDVLNLGPAH
TH_0259|M.thermoresistible__bu PIATTYARDRFGHFDFSGITIRNADHTFDKRLDLEVGGRQVTLLNLGPAH
MAB_2020c|M.abscessus_ATCC_199 ASNMHVLAPLAKPFHFNEVRVRPADTTFSGERALRVGKLDVELHELGPAH
Mflv_4230|M.gilvum_PYR-GCK IIFLKPNTAIIGPYTPIQLPADADPVHFEGELAAVIG----RPCKDVPAS
Mvan_2132|M.vanbaalenii_PYR-1 IIFLKPNTAIIGPNTPIQLPADANPVHFEGELAAVIG----RPCKDVPAS
MLBr_01689|M.leprae_Br4923 VIFLKPNTAIIGPNVPIRLPANASPVHFEGELALVVG----RSCKDVPAV
MUL_1956|M.ulcerans_Agy99 VIFLKSNTAIVGPNVPIRLPANASPVHFEGELAIVIG----RPCKDVSAA
MSMEG_2478|M.smegmatis_MC2_155 TLFARWPSTLTVDGAQIPVPTDEEGLDWEGEVVAWVG----RELSCATAD
: :. :* . .*
Mb0964|M.bovis_AF2122/97 TTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAP
Rv0939|M.tuberculosis_H37Rv TTADSVVHVADAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAP
MMAR_4572|M.marinum_M TAADSVVHVPDAGVLFAGDLLFIGCTPIVWNGPIANWIAACDAMIALDAP
MAV_1057|M.avium_104 TAADSVVHVPDAGVLFGGDLLFIGCTPIVWAGPIANWVAACDAMIALDAP
TH_0259|M.thermoresistible__bu TAADTVIHIPDAGVLFGGDLLFIGCTPIVWGGPIANWIAACDTMLALDAP
MAB_2020c|M.abscessus_ATCC_199 TVGDAVAFLPEHRVLFSGDLLTRGIVKVVWSGSIPNWIVALERIRAFGAK
Mflv_4230|M.gilvum_PYR-GCK RAAENILGYTIANDVSARDQQKKDGQWMRAKG--------HDTFCPVGPW
Mvan_2132|M.vanbaalenii_PYR-1 RAAENILGYTIANDVSARDQQQKDGQWMRAKG--------HDTFCPVGPW
MLBr_01689|M.leprae_Br4923 QAVDCILGYTIGNDMSARDQQRADGQWTRAKG--------HDTFCPVGPW
MUL_1956|M.ulcerans_Agy99 QAVDNILGYTIGNDVSARDQQKSDGQWTRAKG--------HDTFCPVGPW
MSMEG_2478|M.smegmatis_MC2_155 EALDAVVGYSTFNDVTARRAQKITSQWILGKN--------ADRSGPLGPL
. : : . : . . : ....
Mb0964|M.bovis_AF2122/97 TVVPGHGPVTGPDGIRAVRG----YLAHIAEQAEAAYRKGLSLPEAVETI
Rv0939|M.tuberculosis_H37Rv TVVPGHGPVTGPDGIRAVRG----YLAHIAEQAEAAYRKGLSLPEAVETI
MMAR_4572|M.marinum_M IVVPGHGPVTDPDGIRAVRG----YFEHVNEQAEAAYRKGLSLAEAIETV
MAV_1057|M.avium_104 TVVPGHGPVTDPDGIRAVRG----YLVHIAEQAEAAYRKGLSWPEAADTI
TH_0259|M.thermoresistible__bu TVVPGHGPITGPEGIHAVRD----YFEYITEQADDAHRRGLSFIEAADTI
MAB_2020c|M.abscessus_ATCC_199 IVVPGHGPLLVDAEVDAAIDRGIAFWSDLHEQANRLYDQGVPAQDATALI
Mflv_4230|M.gilvum_PYR-GCK IVTDI---DPSDLEIRTEVNG---ELKQHSRTSLMIHDIGAIIEWISSVM
Mvan_2132|M.vanbaalenii_PYR-1 IVTDL---DPSDLEIRTEVNG---DVKQRSRTSLMIHDIGAIIEWISAVM
MLBr_01689|M.leprae_Br4923 IVTDLGPLDPADLELRTAVNG---EVKQHSRTSLMIHGIGAIMEWISAVM
MUL_1956|M.ulcerans_Agy99 IVTDV---DPAGLELRTEVNG---AVKQHARTSLMIHDVGAIVEWISAVM
MSMEG_2478|M.smegmatis_MC2_155 VPAHDVGDLRDGLRVRTRVNG---TTVQDASTDEMVYTVGETLAHISRTL
. : : . : * :
Mb0964|M.bovis_AF2122/97 DLGEYASWLDSERVVVNVYQRYR-ELDPDTPRQDLLALLVMQAEWAARHC
Rv0939|M.tuberculosis_H37Rv DLGEYASWLDSERVVVNVYQRYR-ELDPDTPRQDLLALLVMQAEWAARHC
MMAR_4572|M.marinum_M ELGDYAAWLDSERIVVNIYQRYR-ELDPHTPEVERLALLVMQAEWAAKRV
MAV_1057|M.avium_104 DLGEYATWLDAERVVVNVYQRYR-ELDPHTPQLEVMALLVMQAEWLAKRS
TH_0259|M.thermoresistible__bu DLGPYANWLDAERVVVNVYQRYR-ELDPDTPQLGVITLLTMQAEWHAKRG
MAB_2020c|M.abscessus_ATCC_199 DIAKYPEAMVT--LPIIVTAIYH-ERNPEIPFQSLTQALESISGQLAKAA
Mflv_4230|M.gilvum_PYR-GCK TLLPGDLILTGTPEGVG-------PLEHGDTVSITVEGIGTLSNPVVRKG
Mvan_2132|M.vanbaalenii_PYR-1 TLLPGDLILTGTPEGVG-------PLEHGDSVSITVEGIGTLTNPVVRKG
MLBr_01689|M.leprae_Br4923 TLLPGDLILTGTPAGVG-------PVEDGDIVSITVEGIGTLTNPVVRKG
MUL_1956|M.ulcerans_Agy99 TLLPGDLILTGTPAGVG-------PIEDGDTVSITIEGIDTLTNPVVRKG
MSMEG_2478|M.smegmatis_MC2_155 TLRPGDLLATGTPSGVGYARTPAWLLHPGDHVEVEVEKLGVLRNTIVASQ
: : . : .
Mb0964|M.bovis_AF2122/97 T-----
Rv0939|M.tuberculosis_H37Rv T-----
MMAR_4572|M.marinum_M ------
MAV_1057|M.avium_104 ------
TH_0259|M.thermoresistible__bu AR----
MAB_2020c|M.abscessus_ATCC_199 AGPK--
Mflv_4230|M.gilvum_PYR-GCK KS----
Mvan_2132|M.vanbaalenii_PYR-1 RS----
MLBr_01689|M.leprae_Br4923 KS----
MUL_1956|M.ulcerans_Agy99 KS----
MSMEG_2478|M.smegmatis_MC2_155 RRPAHN