For questions or suggestions e-mail us at: ioerger@cs.tamu.edu
KLGPTMEFDHIQPAIRKRFTSAADIPKEVFSDPDVYREELTRIFYGPYWHPIAHRAELAERNAFRTRWLA DVPLLMVRDGDDRIRVFVNSCAHRGTLLEQRRCGVAERFECPYHRWIFNNDGRFAGAPRRMQFRPDFREE DYGLRELHVVEAWGLIFVSMAAQPPPFDDYLGDSADPLRDCMVDDGDLTLLGYQTVVFQSNWKTYIDNDP YHAPLLHSAFKLLNWQGGSGNVLVSEPYGHMSILYDAQPYVDNGFLADPSVVTRMGDDSRARVIALRPVT GIVRHVDTINIRYARPLGVDRTEVRYTFFGHASDSEDFARHRVRQSSNLLGPSGFISIEDAAVYNRVQAT ARDGGYQRFVAGVGRPLSESSQNDEVANTGWWAHYQEVMEFC*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. gilvum PYR-GCK | Mflv_0563 | - | - | 100% (393) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0658 | - | 0.0 | 100.00% (393) | Rieske (2Fe-2S) domain-containing protein |
| M. gilvum PYR-GCK | Mflv_0662 | - | 4e-28 | 33.76% (237) | ring hydroxylating dioxygenase, alpha subunit |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3186c | - | 9e-16 | 26.77% (198) | dioxygenase |
| M. tuberculosis H37Rv | Rv3161c | - | 2e-15 | 26.26% (198) | dioxygenase |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_4370c | - | 3e-25 | 27.15% (361) | putative large terminal subunit of phenylpropionate dioxygenase |
| M. marinum M | MMAR_4744 | - | 1e-15 | 26.27% (255) | dioxygenase |
| M. avium 104 | MAV_1901 | - | 2e-26 | 26.42% (352) | dioxygenase large subunit |
| M. smegmatis MC2 155 | MSMEG_1908 | - | 3e-26 | 24.87% (398) | benzoate 1,2-dioxygenase alpha subunit |
| M. thermoresistible (build 8) | TH_2967 | - | 4e-39 | 28.86% (395) | PUTATIVE putative Rieske (2Fe-2S) domain protein |
| M. ulcerans Agy99 | MUL_3820 | - | 8e-15 | 31.36% (169) | ring-hydroxylating dioxygenase, large terminal subunit |
| M. vanbaalenii PYR-1 | Mvan_0492 | - | 0.0 | 100.00% (393) | Rieske (2Fe-2S) domain-containing protein |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_0563|M.gilvum_PYR-GCK ---------MKLGPTMEFDHIQPAIRKRFTS---AADIPKEVFSDPDVYR
Mvan_0492|M.vanbaalenii_PYR-1 ---------MKLGPTMEFDHIQPAIRKRFTS---AADIPKEVFSDPDVYR
Mb3186c|M.bovis_AF2122/97 --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
Rv3161c|M.tuberculosis_H37Rv --------MLSTDNRAELGDILTDIGDYLDDNPPALSLPPAAYTSSELWQ
TH_2967|M.thermoresistible__bu ---------MPVFPTSD-----------------GSRIPYEVYSSQAVYD
MAB_4370c|M.abscessus_ATCC_199 MASLPRPVAVAQGALCRPQALVRDIDVSGLVDSHGGLLDRVIFTDGELYR
MMAR_4744|M.marinum_M ---------MPHFTKPAAGSWTQNYPELG-----TAPVDYTDSIDPQYFA
MAV_1901|M.avium_104 ------MTTLETAPTLDEFDVSKIVR--------SDRVHGSVYTSPEIFR
MSMEG_1908|M.smegmatis_MC2_155 ---------MTTHLSPDHLENVLADAVIEDPAAGVYRANRRIFTDDEIFE
MUL_3820|M.ulcerans_Agy99 ---------MDRDQLIDLTRRALKLARDKTTDLAPHQHAVDAREYTSLDR
:
Mflv_0563|M.gilvum_PYR-GCK EELTRIFYGPYWHPIAHRAELAERNAFRTRWLADV----PLLMVRDGDDR
Mvan_0492|M.vanbaalenii_PYR-1 EELTRIFYGPYWHPIAHRAELAERNAFRTRWLADV----PLLMVRDGDDR
Mb3186c|M.bovis_AF2122/97 LERERIFN-RSWMLVAHVDQLAKTGDYVTVSVAGE----PVMVVRDVDGQ
Rv3161c|M.tuberculosis_H37Rv LERERIFN-RSWMLVAHVDQVAKTGDYVTVSVAGE----PVMVVRDVDGQ
TH_2967|M.thermoresistible__bu REQERLFRGPIWNFLALEAELPNPFDFKSTFVGDT----PVVVTRDGEGR
MAB_4370c|M.abscessus_ATCC_199 QELRRVFA-PSWLFLAHADQFHRPGDFFTTYMGED----PVIVSMDKNRR
MMAR_4744|M.marinum_M DEQRAIFR-RTWLNVGRIERLPRTGSYFTKELPSTGAGMSVIVVKGKDGV
MAV_1901|M.avium_104 REMDTIFK-TGWVYVAHESEVSERGDYLTRMIGYD----PVVVVRGKDGV
MSMEG_1908|M.smegmatis_MC2_155 LEMKHIFE-GNWVYLAHESQVPNPGDYFTTYIGRQ----PVVITRDRDGD
MUL_3820|M.ulcerans_Agy99 HERDRAMVLDSPQLVGYSSELPGPDTYRTKTIMGR----SILLTRAADRA
* : :. .. : : : .::: :
Mflv_0563|M.gilvum_PYR-GCK IRVFVNSCAHRGTLLEQ------RRCGVAERFECPYHRWIFNNDGRFAGA
Mvan_0492|M.vanbaalenii_PYR-1 IRVFVNSCAHRGTLLEQ------RRCGVAERFECPYHRWIFNNDGRFAGA
Mb3186c|M.bovis_AF2122/97 LHALSPICRHRLMLMVE------PGAGRIDTLTCQYHLWRYGLDGRLRGA
Rv3161c|M.tuberculosis_H37Rv LHALSPICRHRLMLMVE------PGAGRIDTLTCQYHLWRYGLDGRLRGA
TH_2967|M.thermoresistible__bu LAAWVNRCAHRGATVCR------EQTGNTRSHVCVYHQWSYNAAGDLEGV
MAB_4370c|M.abscessus_ATCC_199 IRAFLNSCRHRGARVCR------ADYGVTRGFTCTYHGWAYDLEGKLISV
MMAR_4744|M.marinum_M IRAFHNICRHRGNKLVWNDFPHEETAGTCRQFTCKYHAWRYGLDGELTFI
MAV_1901|M.avium_104 VRVVLNRCTHRANKLCN------AEKGNANSFRCPYHGWTFNNTGALQGV
MSMEG_1908|M.smegmatis_MC2_155 LHCLINACAHRGAMICR------RKTDNRTTLTCPFHGWTFRNDGTLLKV
MUL_3820|M.ulcerans_Agy99 VRAFDNVCLHRQSRVAT-------GCGTALRFTCPYHGWTYDNGGRLVGL
: * ** : . * :* * : * :
Mflv_0563|M.gilvum_PYR-GCK PRRMQFR-----PDFREE--DYGLREL-HVVEAWGLIFVSMAAQP-PPFD
Mvan_0492|M.vanbaalenii_PYR-1 PRRMQFR-----PDFREE--DYGLREL-HVVEAWGLIFVSMAAQP-PPFD
Mb3186c|M.bovis_AF2122/97 P-HMAAN-----LDFNRR--ECRLPQF-AVATWNGLVWINLDADA-EPIA
Rv3161c|M.tuberculosis_H37Rv P-HMAAN-----LDFNRR--ECRLPQF-AVATWNGLVWINLDADA-EPIA
TH_2967|M.thermoresistible__bu PFRRGANGTGMPKSFDPT--QNGLQKL-RVESYRGLVFGTFDEAT-PDLL
MAB_4370c|M.abscessus_ATCC_199 PNEEGYP-----DSFSTD--DWGLVEVPHVDSYHGLIFGTWNPAP-VGLR
MMAR_4744|M.marinum_M QQEDEFF------DVDKS--QYGLKSV-RCEVWEGFIFINLDPAA-APLS
MAV_1901|M.avium_104 PMREGYG-----EAFNEIRAELGLAQAPRVDSYGGFIFASLAAEG-VSLR
MSMEG_1908|M.smegmatis_MC2_155 KDPDGAG---YPDTFNSN-GSHNMTNVARFEGYRGFLFGSLNPDV-LPLT
MUL_3820|M.ulcerans_Agy99 PGREGFP------GIAVG--SDGLTEL-PATEFAGFLWVSLHPAARLDVP
. . : . *::: . .
Mflv_0563|M.gilvum_PYR-GCK DYLGDSADPLRDCMVDD--GDLTLLG-YQTVVFQSNWKTYID---NDPYH
Mvan_0492|M.vanbaalenii_PYR-1 DYLGDSADPLRDCMVDD--GDLTLLG-YQTVVFQSNWKTYID---NDPYH
Mb3186c|M.bovis_AF2122/97 AHLDLTDDEFAGYRLG----EMVQVE-SWSHEWRANWKVAAE---NGHEN
Rv3161c|M.tuberculosis_H37Rv AHLDLTDDEFAGYRLG----EMVQVE-SWSHEWRANWKVAAE---NGHEN
TH_2967|M.thermoresistible__bu AYLGNQTPGLDRIFHK----PIEYLG-CTRQRIRGNWKLYLENV-RDGYH
MAB_4370c|M.abscessus_ATCC_199 ESLGDMAWYMDAMLDHDSEGTVVVGG-VHKWVLDGNWKLAAEQFATDWYH
MMAR_4744|M.marinum_M EYLGSMAKGLQGYPFH----EMTEVY-SYRAEVGSNWKLFIDAF-AEFYH
MAV_1901|M.avium_104 EHLGNATHAIDRLLNLSPNRQIDLRANWMKHLHHANWKMVVENN-VDGYH
MSMEG_1908|M.smegmatis_MC2_155 EHLGDTTTVIDMLVDQSPDGLEVLRG-ASTYTYDGNWKVQAENG-ADGYH
MUL_3820|M.ulcerans_Agy99 AHLGPLAGELQSWGIG----RWSPLG-EKVLGSPIDWKLATDTF-AENYH
*. : :** : :
Mflv_0563|M.gilvum_PYR-GCK APLLHSAFKLLNWQGGS------------------GNVLVSEPYGHMSIL
Mvan_0492|M.vanbaalenii_PYR-1 APLLHSAFKLLNWQGGS------------------GNVLVSEPYGHMSIL
Mb3186c|M.bovis_AF2122/97 YHVLGLHRQTLEPFVPG------------------GGDLDVRQYSRWALR
Rv3161c|M.tuberculosis_H37Rv YHVLGLHRQTLEPFVPG------------------GGDLDVRQYSRWALR
TH_2967|M.thermoresistible__bu ASLLHAFHSTFNLVRSGQRVKIVVGDTHEVHNC--GDLFYTEDPTKGAAY
MAB_4370c|M.abscessus_ATCC_199 VNMSHASALMVMAPQGKAPKDEIVHRTGRQYVD--PNGHGAGFPVHPKNR
MMAR_4744|M.marinum_M APVLHQGQYTKEEAAKIMKYG---FEALHYELA--GPHAMISVWGGQSPP
MAV_1901|M.avium_104 ALFTHASVYDAIKPAKVSHVPTKVDVLVRDLGNGHSEIDYSDEYRKLDEE
MSMEG_1908|M.smegmatis_MC2_155 VTATHWNYAATTSRRTCGESKNDTKALDAGSWGKSGGGYWSFPHGHLCLW
MUL_3820|M.ulcerans_Agy99 FATVHRRTFATIAR---------------------SNCTVFDSYG-----
Mflv_0563|M.gilvum_PYR-GCK ---YDAQPYVDNGFLADPSVVTRMGDDSRAR----VIALRPVTGIVRHVD
Mvan_0492|M.vanbaalenii_PYR-1 ---YDAQPYVDNGFLADPSVVTRMGDDSRAR----VIALRPVTGIVRHVD
Mb3186c|M.bovis_AF2122/97 ---LRVPFTVP---VEAKSLQLNEVQKSNLV----VLWTFPNSALAIAGE
Rv3161c|M.tuberculosis_H37Rv ---LRVPFTVP---VEAKSLQLNEVQKSNLV----VLWTFPNSALAIAGE
TH_2967|M.thermoresistible__bu ---ANVTSFKEGLRLENPELVENWQEFDEIIT-NHLQCIFPQLVVMQIQN
MAB_4370c|M.abscessus_ATCC_199 ---FDARVVHQWTDYGALRERLGEARVQGPLT-NGHATVFPNLSYLPVNG
MMAR_4744|M.marinum_M ---ADLKMVKPMDRVLRSGLFGPWDRPAPIT---DLEELPPGINPTKAKQ
MAV_1901|M.avium_104 ---FVWYGRIPRAKLAKYVDALEKAYGPEQAH-DALVVGPPHTLIWPNLF
MSMEG_1908|M.smegmatis_MC2_155 TWAANPQDRPLWDRLDELKARFGDAKGEFMVKGSRNLCLYPNVYVMDQFS
MUL_3820|M.ulcerans_Agy99 ---PHHRLIFPLNTILELENVP-EGQWDPFHHMVVIYALFPNVVLSVTIA
*
Mflv_0563|M.gilvum_PYR-GCK T-INIRYARPLGVDRTEVRYTFFGHASDSEDFARHRVRQSSNLLGPSGFI
Mvan_0492|M.vanbaalenii_PYR-1 T-INIRYARPLGVDRTEVRYTFFGHASDSEDFARHRVRQSSNLLGPSGFI
Mb3186c|M.bovis_AF2122/97 R-VVWFGFIPQSIDRVQV----LGGVLTTPELAADAAATAQTSQFVMAMI
Rv3161c|M.tuberculosis_H37Rv R-VVWFGFIPQSIDRVQV----LGGVLTTPELAADAAATAQTSQFVMAMI
TH_2967|M.thermoresistible__bu V-LAVRNVLPKGVDEFELIFNFFGYADDTPEMRRHRIAQFN-LVGPAGLI
MAB_4370c|M.abscessus_ATCC_199 ---SVRIWHPKGPDKMEVWAWTIVDRSMPEEVRDAQRLYNLRTFGPTGIF
MMAR_4744|M.marinum_M WGIDSFLFFPNFMLLIWEPGWYLTYHYWPTTVDKHIFETTLYFVPPRNAR
MAV_1901|M.avium_104 LAEMNVMFVEPQAPDRTIAYTTAVLIPGQDELNERTLRRSEGAMGPAGFL
MSMEG_1908|M.smegmatis_MC2_155 T--QIRHFRPIAPDKTEVTIYCIAPKGESAAARAHRIRQYEDFFNASGMA
MUL_3820|M.ulcerans_Agy99 N----GELFRIYPGDRPGSSITVHQNSTPLDLSDESVATGAQAVFDYAHA
Mflv_0563|M.gilvum_PYR-GCK S-IEDAAVYNRVQATARDG--------GYQR-FVAGVGRP-LSESSQ---
Mvan_0492|M.vanbaalenii_PYR-1 S-IEDAAVYNRVQATARDG--------GYQR-FVAGVGRP-LSESSQ---
Mb3186c|M.bovis_AF2122/97 N-DEDRLGLEAVQVGAGSR--------FAERGHLSSKEWPGMLAFYR---
Rv3161c|M.tuberculosis_H37Rv N-DEDRLGLEAVQVGAGSR--------FAERGHLSSKEWPGMLAFYR---
TH_2967|M.thermoresistible__bu S-MEDGEAIELVQRATAVGPA------GEAIALMALEADEEHRNQHP---
MAB_4370c|M.abscessus_ATCC_199 E-QDDGENWSEVQSISRGFVTN-----SVALNYQMGLDSEREDGRHPGLT
MMAR_4744|M.marinum_M ERLAQELAAVTFKEYALQDSNT-----LEATQTMIGTGVVKDFPLCDQEI
MAV_1901|M.avium_104 IADDGEIGMRNQAGLAAELPEW-----LVLARGTENDITDDSGIINRD--
MSMEG_1908|M.smegmatis_MC2_155 TPDDLEEFRSCQLTFRAQAAPWNDMSRGAQHWLSGPDPVAESLGMHGIVS
MUL_3820|M.ulcerans_Agy99 TVRDEDYRLAETLQTNLES--------GARQRLVFGRNEPGLQHRHIT--
Mflv_0563|M.gilvum_PYR-GCK ---NDEVA-----NTGWWAHYQEVMEFC-------
Mvan_0492|M.vanbaalenii_PYR-1 ---NDEVA-----NTGWWAHYQEVMEFC-------
Mb3186c|M.bovis_AF2122/97 ---NLAMA-----LVGDHPGAS-------------
Rv3161c|M.tuberculosis_H37Rv ---NLAMA-----LVGDHPGAS-------------
TH_2967|M.thermoresistible__bu ---ISEDA-----IRRFWVGYQKLMGL--------
MAB_4370c|M.abscessus_ATCC_199 SELYSDAA-----GRSFYARWRDLMNTKAWHEEHV
MMAR_4744|M.marinum_M LLRHLHKM-----TGDYVKEYRNNGHAK-------
MAV_1901|M.avium_104 --KSAETP-----QRGFYAQWAAVVGGGVRA----
MSMEG_1908|M.smegmatis_MC2_155 SGLRNEDEGLYPVQHGYWLETLRRAVTSNPTEH--
MUL_3820|M.ulcerans_Agy99 -----------------WAQALETPG---------