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VPESVPASSAHQPATYDLVIHRAHVFDGYQAHTGLRDVGVNGGQIADVSAEPLRGETEVDAAEGWVLPGL IDTHVHFYDVAAVTDPASMRTFEENELADRLKSFLRHGITTIKSVGDPTDEVLRTRSRIAAGTLRGPRFL ATGCGITGRDGHPASTVFANNPWARQRFTREVDSAAQMQDVVHHLADRKVDAIKLLSEGACACATSPEYL WRNPAFPTAVELTRLSPELLKAGIDTAHERGLRVTVHTTQQAAAREAIEAGADGLEHGVTVEPITDHALV DLMLERDTTYTPTLWIDDAAHPDTRGNLKKVADAGVTIVMGSDTFSGRGLFGANTLEEAELMVAAGMTPL QSLVAGTSAAARQCVRPDLGAVAPGKRADLVVLTADPTRDIGNLRNLAMTILDGEIVVDNRQGTT
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_6887 | - | - | 100% (415) | amidohydrolase family protein |
| M. smegmatis MC2 155 | MSMEG_4392 | - | 4e-27 | 28.38% (437) | organophopsphate acid anhydrase |
| M. smegmatis MC2 155 | MSMEG_4842 | - | 1e-20 | 24.62% (398) | amidohydrolase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0076 | - | 5e-20 | 26.07% (399) | hypothetical protein Mb0076 |
| M. gilvum PYR-GCK | Mflv_2437 | - | 3e-21 | 26.63% (398) | amidohydrolase |
| M. tuberculosis H37Rv | Rv0074 | - | 5e-20 | 26.07% (399) | hypothetical protein Rv0074 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_3236c | - | 3e-14 | 26.25% (381) | amidohydrolase |
| M. marinum M | MMAR_4746 | - | 1e-23 | 27.90% (405) | imidazolonepropionase |
| M. avium 104 | MAV_0903 | - | 5e-22 | 26.62% (402) | Xaa-Pro dipeptidase |
| M. thermoresistible (build 8) | TH_3200 | - | 4e-26 | 28.57% (434) | PUTATIVE amidohydrolase |
| M. ulcerans Agy99 | MUL_0705 | - | 5e-18 | 25.30% (411) | imidazolonepropionase |
| M. vanbaalenii PYR-1 | Mvan_4215 | - | 1e-23 | 27.39% (398) | amidohydrolase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2437|M.gilvum_PYR-GCK ------------MPLTTLKAAGLLDVDAGEIIRPGIVHVDGDRIVSIGGD
Mvan_4215|M.vanbaalenii_PYR-1 --------------MLTLKAAGLLDVVSGEIIRPGVVTVDGDRIVSLGGT
MMAR_4746|M.marinum_M --------------MLTLKAAGLLDVDAGEILAPAVVRVEGDRIAGVGGD
MAV_0903|M.avium_104 --------------MQTIKAAGLFDVDAGEIVRPGLLRVDGDRIVGVGDS
MUL_0705|M.ulcerans_Agy99 ----------MEPNELHISNVKVFDSVDGRITGPVDVTVRGPVIASIAPA
TH_3200|M.thermoresistible__bu -----------VVSKLLLHNALLIDGTGVPPVRDAAVLIDDGRIGWAGPG
Mb0076|M.bovis_AF2122/97 MGDLSISQVSARPGRIGIRARQMFDGYR-FQRGPVLVVVEDGRISAVDFA
Rv0074|M.tuberculosis_H37Rv MGDLSISQVSARPGRIGIRARQMFDGYR-FQRGPVLVVVEDGRISAVDFA
MAB_3236c|M.abscessus_ATCC_199 -------------------MRAHLRGRTLPSEEPIDLWVHDGVISIEPIA
MSMEG_6887|M.smegmatis_MC2_155 ----MPESVPASSAHQPATYDLVIHRAHVFDGYQAHTGLRDVGVNGGQIA
: : . :
Mflv_2437|M.gilvum_PYR-GCK AP--PDAE---VIDLGDAILMPGLMDMEVNLLMGGRGETPGLSQVQDDPA
Mvan_4215|M.vanbaalenii_PYR-1 PP--PDVE---VIDLGDSILLPGLMDMEVNLLMGGRGENPGLSQVQDDPA
MMAR_4746|M.marinum_M ----PEGD---VIDLGDQILLPGLMDMEVNLLMGGRGEKPGLSQVQDDPP
MAV_0903|M.avium_104 GPTGADDN---VIDLGEAILLPGLMDMEVNLLMGGRGEKPGLSQVQDDPP
MUL_0705|M.ulcerans_Agy99 GAAALSGP---AIDGTGKTLLPGLIDAHWHAMFT---TIPAALAQVSEFG
TH_3200|M.thermoresistible__bu DDIPPSADGATRIDVGGNAVCPGFFDCHVHFSLPGTRGSVYERALVTES-
Mb0076|M.bovis_AF2122/97 GSACPDMN---LVDLGESTLLPGLVDAHAHLCWDPDGRPEDLAG--DPHA
Rv0074|M.tuberculosis_H37Rv GSACPDMN---LVDLGESTLLPGLVDAHAHLCWDPDGRPEDLAG--DPHA
MAB_3236c|M.abscessus_ATCC_199 DADTVFDG---------GWLLPGLVDAHCHVGIG------------PQGP
MSMEG_6887|M.smegmatis_MC2_155 DVSAEPLRGETEVDAAEGWVLPGLIDTHVHFYDVAAVTDPASMR-----T
: **:.* . :
Mflv_2437|M.gilvum_PYR-GCK TRVLRAVGNARRTLRAGFTTVRNLGLFVKTGGYLLDVALGKAIAAGWIDG
Mvan_4215|M.vanbaalenii_PYR-1 TRVLRAVGNARRTLRAGFTTVRNLGLFVKTGGYLLDVALGKAIDAGWIDG
MMAR_4746|M.marinum_M TRMLRAVGNARRTLRAGFTTVRNLGLFVKTGGYLLDVALGKAIDAGWIDG
MAV_0903|M.avium_104 TRVLRAVGNARRTLRAGFTTVRNLGLFVKTGGYLLDVALAKAIDAGWIDG
MUL_0705|M.ulcerans_Agy99 YVVARAVVSARETLLRGFTTVRDLGGPVFG--------VKQAIDEGSIPG
TH_3200|M.thermoresistible__bu YHTFQLLDRLKVTLENGVTTARDLMGIDTG--------VRQAVADGLIPG
Mb0076|M.bovis_AF2122/97 VLVGRARRHAAAALRSGITTIRDLGDRDYAA-----LALREEYRQKTTVG
Rv0074|M.tuberculosis_H37Rv VLVGRARRHAAAALRSGITTIRDLGDRDYAA-----LALREEYRQKTTVG
MAB_3236c|M.abscessus_ATCC_199 TSLEEAVAQAEAEREVGALLLRDAGSPVDTRG-----------FDEREDL
MSMEG_6887|M.smegmatis_MC2_155 FEENELADRLKSFLRHGITTIKSVGDPTDEVLR-----TRSRIAAGTLRG
. * :.
Mflv_2437|M.gilvum_PYR-GCK PRVIPAGHAITPTGGHLDPTMFAAFMPGVL------ELTIEEGIANGVDE
Mvan_4215|M.vanbaalenii_PYR-1 PRVIPAGHAITPTGGHLDPTMFAAFMPGVL------ELTVEEGIANGVDE
MMAR_4746|M.marinum_M PRIVPAGHAITPTGGHLDPTMFAAFAPHVL------ELTIEEGIANGVDE
MAV_0903|M.avium_104 PRVVPAGHAITPTGGHLDPTMFAAFAPHAL------ELTVEEGIANGIDE
MUL_0705|M.ulcerans_Agy99 PRIYPAGGFISQSGGHGDFRLPNEVPRGVCGHLSYTEIIGAAVIADGEAE
TH_3200|M.thermoresistible__bu PRLQVAVNMLSQTAGHADFRLPSGIDATAMMG---------GARVDSVDD
Mb0076|M.bovis_AF2122/97 PELVVSGPPLTRSGGHCWFLG---------------------GVADSVEE
Rv0074|M.tuberculosis_H37Rv PELVVSGPPLTRSGGHCWFLG---------------------GVADSVEE
MAB_3236c|M.abscessus_ATCC_199 PRIIRAGRHLAKPKRYIPGLP---------------------LDIEDDAQ
MSMEG_6887|M.smegmatis_MC2_155 PRFLATGCGITGRDGHPASTVFANNPWARQR---------FTREVDSAAQ
*.. : :: : :. :
Mflv_2437|M.gilvum_PYR-GCK IRKAVRYQIKHGAELIKVCCSGGVMSLTG------------EAGAQHYSD
Mvan_4215|M.vanbaalenii_PYR-1 IRKAVRYQIKHGAQLIKVCCSGGVMSLTG------------EAGAQHYSD
MMAR_4746|M.marinum_M IRKAVRYQIKHGAQLIKVCCSGGVMSLTG------------PPGAQHYSD
MAV_0903|M.avium_104 IRKAVRYQIKHGAELIKVCCSGGIMSLTG------------PPGAQHYSD
MUL_0705|M.ulcerans_Agy99 VLRGAREMLRRGASQLKLMAGGGVTSAYD------------PLDVAQFTE
TH_3200|M.thermoresistible__bu VRRRVRDLIAQGADLIKVASSGGVSSPTD------------QPDWLGMRT
Mb0076|M.bovis_AF2122/97 LVDAVQERAARGADWIKVMATGGFVTTAS------------DPWQPQYGS
Rv0074|M.tuberculosis_H37Rv LVDAVQERAARGADWIKVMATGGFVTTAS------------DPWQPQYGS
MAB_3236c|M.abscessus_ATCC_199 LPEAVALQARWGDGWVKLVG--DWIDRAV------------GDLAPLWSD
MSMEG_6887|M.smegmatis_MC2_155 MQDVVHHLADRKVDAIKLLSEGACACATSPEYLWRNPAFPTAVELTRLSP
: . :*:
Mflv_2437|M.gilvum_PYR-GCK EELRVIVDEAHRRGLR-VAAHTHGAEAVKHAIDCGIDCIEHGFLMDDEAI
Mvan_4215|M.vanbaalenii_PYR-1 EELRVIVDEAHRRGLR-VAAHTHGAEAVKHAVECGIDCIEHGFLMDDEAI
MMAR_4746|M.marinum_M EELRAIVDEAHRRGLR-VAAHTHGADAVKHAVIAGIDCIEHGFLVDDEAI
MAV_0903|M.avium_104 EELRAIVDEAHRRGMR-VAAHTHGADAVKHAVAAGIDCIEHGFLIDDEAI
MUL_0705|M.ulcerans_Agy99 TEMRAAVEAAENWGTY-VTVHAYTPRAIRAAIAAGVRCVEHGQLIDEPTA
TH_3200|M.thermoresistible__bu EMVAAVVEECQAYGGRPVAAHAIGYAGVKAAVEGGVTSIEHGYALDDELR
Mb0076|M.bovis_AF2122/97 GQLAAVVAAAEQVGLP-VTAHAHATAGIAAAVAAGVDGIEHCTFLSEGSA
Rv0074|M.tuberculosis_H37Rv GQLAAVVAAAEQVGLP-VTAHAHATAGIAAAVAAGVDGIEHCTFLSEGSA
MAB_3236c|M.abscessus_ATCC_199 KILKQAIDAAHANGAR-VTAHVFGEDALPGLIHAGIDCIEHGTGLTDDTI
MSMEG_6887|M.smegmatis_MC2_155 ELLKAGIDTAHERGLR-VTVHTTQQAAAREAIEAGADGLEHGVTVEPITD
: : .. * *:.*. . : * :** :
Mflv_2437|M.gilvum_PYR-GCK QMLVDNDRFLVSTRRLAEY----MDVSKAPKELQAKAAEMFPKARTSIKA
Mvan_4215|M.vanbaalenii_PYR-1 QMLVDNDRFLVSTRRLAEY----MDVSKAPPELQAKAAEMFPKARTSIKA
MMAR_4746|M.marinum_M AMMVEHGTFLVSTRRLADADA--MDISHAPPELQAKAAEMFPRSRESILA
MAV_0903|M.avium_104 ASMVEHGTFLVSTRRLAEG----MDVSHAPPELQAKAAQMFPKSRTSILA
MUL_0705|M.ulcerans_Agy99 ALLAEKDIWWCLQPFLDDE-----DAVAVPPPSVPKFKQMVAGTDTAYQL
TH_3200|M.thermoresistible__bu RRMVDQGTYLVPTLLETMH------DVEVSPAAKAKSAHWHEIAHRAVGE
Mb0076|M.bovis_AF2122/97 AASPDVVEAIVAQGVWCGMT-----IPRVYPEMPENLVAVVQDGWRNIRR
Rv0074|M.tuberculosis_H37Rv AASPDVVEAIVAQGVWCGMT-----IPRVYPEMPENLVAVVQDGWRNIRR
MAB_3236c|M.abscessus_ATCC_199 ALMVEHGTALVPTVINIENFPGIADKAGKYPKYAQHMRDLYAGLHTTFGN
MSMEG_6887|M.smegmatis_MC2_155 HALVDLMLERDTT----------------YTPTLWIDDAAHPDTRGNLKK
:
Mflv_2437|M.gilvum_PYR-GCK AYEAGVKIAVGTDAP---AIPHGKNADELVTLVDWG-MPEAAVLRAATVV
Mvan_4215|M.vanbaalenii_PYR-1 AYEAGVKIAVGTDAP---AIPHGKNADELVTLVDWG-MPPAAVLRAATVV
MMAR_4746|M.marinum_M AYQAGVKIAIGTDAP---AIPHGRNADELVTLVHWG-MSPMAVLRAATVT
MAV_0903|M.avium_104 AHRAGVKIAVGTDAP---AIPHGRNADELVTLVEWG-LPPVAVLRAATVT
MUL_0705|M.ulcerans_Agy99 AIKHGVKIAFGTDTLFD-AKLASRQGAQLAKLTRW--FTPAEVLQQATIR
TH_3200|M.thermoresistible__bu SAAAGVKIALGTDAG-L-SPDHGTNLAELGLLVRFGGLTPMEAIVAGTRT
Mb0076|M.bovis_AF2122/97 LIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHG-FTSTEVLTGATAA
Rv0074|M.tuberculosis_H37Rv LIDAGARVALSTDAGVAPGRRHDVLPDDLVYLSRHG-FTSTEVLTGATAA
MAB_3236c|M.abscessus_ATCC_199 AYDAGIPIYAGTDAG--GSIAHGRISDEVAALTRIG-MSPTDALGAASWR
MSMEG_6887|M.smegmatis_MC2_155 VADAGVTIVMGSDTFSGRGLFGANTLEEAELMVAAG-MTPLQSLVAGTSA
* : .:*: . : : :. : .:
Mflv_2437|M.gilvum_PYR-GCK AADLINRSDS-------LGRLAEGYIADIIAVPGDPLADIRVTQA----V
Mvan_4215|M.vanbaalenii_PYR-1 AADLINRSDS-------LGRIAEGYLADIIAVPGDPLADIGVTKN----V
MMAR_4746|M.marinum_M AAELINVTD--------RGRLAAGQLADIIAVAGNPLDDITVTRN----V
MAV_0903|M.avium_104 AAELINSSD--------RGRLAEGYLADVIAVPGNPLEDITVTQH----V
MUL_0705|M.ulcerans_Agy99 NAELLAMSGPRNPYPGRLGVVAEGALADLILVDGDPIADISLIARPDETF
TH_3200|M.thermoresistible__bu SAQLCGLDR-------ELGTLEPGKLADVVVVRGNPLDDIASVGNPD-NI
Mb0076|M.bovis_AF2122/97 AAASCGLGHR-------KGRIAPGYDADLLAVAAGVDHDPAGLCD----V
Rv0074|M.tuberculosis_H37Rv AAASCGLGHR-------KGRIAPGYDADLLAVAAGVDHDPAGLCD----V
MAB_3236c|M.abscessus_ATCC_199 ARSWLGRPG-----------LEHGAPADLLGYRADPRTGPGVLAS----P
MSMEG_6887|M.smegmatis_MC2_155 AARQCVRPD--------LGAVAPGKRADLVVLTADPTRDIGNLRN----L
: * **:: .. .
Mflv_2437|M.gilvum_PYR-GCK NFVMKDGKVFKNDVTH-------
Mvan_4215|M.vanbaalenii_PYR-1 TFVMKGGKVYKNEHATDTN----
MMAR_4746|M.marinum_M GFVMKGGKVYVHQD---------
MAV_0903|M.avium_104 SFVMKGGKVYVHNQN--------
MUL_0705|M.ulcerans_Agy99 TTIIKNGRVVKGAAS--------
TH_3200|M.thermoresistible__bu LLVLKEGRPAKDRGGFFDQRH--
Mb0076|M.bovis_AF2122/97 KAVWRSGTQVPLQASAVGYNTPS
Rv0074|M.tuberculosis_H37Rv KAVWRSGTQVPLQASAVGYNTPS
MAB_3236c|M.abscessus_ATCC_199 DLVMLRGQIR-------------
MSMEG_6887|M.smegmatis_MC2_155 AMTILDGEIVVDNRQGTT-----
*