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MHGLMQDRPLSLPLLMSGLDGRFSHKTVTTNHPDAPTVATYGAVAQRVRRLAGVLDALAVPVGARVGSFG WNTQRHLELYLAVPCTNRVLHTVNHRLFADDIVYIVNDAADDVLFVDRSLLDVVWPLVGRFTTVRHVIVM NDGSGPQIPDGAHDYEHLLAEARPVARFEVSDERTAAALCYTSGTTGRPKGVLYDHRSIVLHAMSLLMAD AFGIGEADVVMPIVPMFHVNAWGLPYAAVMSGASLVLPGPAMQPASLAETLDTAGVTFAAAVTTVWRNVL PHLSGRSLPSVRRLVSGGGPLPESLSRRYASEIGVPLCSSWGMTETSPLVCSARIPTGHSGDEVETLCLP GVSIPLVQLRLQRDDGSFAPHDGKSSGELQVAGPTIASGYYGATDGSAAFTEDGWLRTGDVATIDAHGLV RIVDRIKDLIKSGGEWISSVELENAIMTCPAVAEAAVVGAPHERWGERPLAFVVPAAGHDATPDLVRDHL QQRVARWWIPDDIVIVDELPKTATGKISKRALRQNLCAPAG
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_5348 | - | - | 100% (531) | medium-chain fatty acid-CoA ligase |
| M. smegmatis MC2 155 | MSMEG_5291 | - | e-111 | 42.83% (537) | acyl-CoA synthetase |
| M. smegmatis MC2 155 | MSMEG_4065 | - | 3e-62 | 33.20% (494) | feruloyl-CoA synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1087 | fadD14 | 1e-119 | 43.58% (530) | acyl-CoA synthetase |
| M. gilvum PYR-GCK | Mflv_1585 | - | 1e-115 | 42.41% (540) | AMP-dependent synthetase and ligase |
| M. tuberculosis H37Rv | Rv1058 | fadD14 | 1e-119 | 43.58% (530) | acyl-CoA synthetase |
| M. leprae Br4923 | MLBr_01051 | xclC | 1e-45 | 35.58% (385) | acyl-CoA synthetase |
| M. abscessus ATCC 19977 | MAB_2080 | - | 1e-111 | 42.61% (528) | acyl-CoA synthetase |
| M. marinum M | MMAR_4412 | fadD14 | 1e-116 | 43.40% (530) | medium chain fatty-acid-CoA ligase FadD14 |
| M. avium 104 | MAV_1186 | - | 1e-119 | 44.80% (538) | acyl-CoA synthetase |
| M. thermoresistible (build 8) | TH_2961 | - | 1e-113 | 43.23% (532) | PUTATIVE AMP-dependent synthetase and ligase |
| M. ulcerans Agy99 | MUL_0229 | fadD14 | 1e-117 | 43.40% (530) | acyl-CoA synthetase |
| M. vanbaalenii PYR-1 | Mvan_0905 | - | 1e-149 | 50.75% (530) | AMP-dependent synthetase and ligase |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_5348|M.smegmatis_MC2_155 MHGLMQDRPLSLPLLMSGLDGRFSHKTVTTNHP-DAPTVATYGAVAQRVR
Mvan_0905|M.vanbaalenii_PYR-1 MLGLMQNRPLVLPHIFHRAERYFGHKQVITATAGGAVAMSTVADVCGQVR
Mb1087|M.bovis_AF2122/97 MYGTMQDFPLTITAIMRHGCGVHGRRTVTTATGEG-YRHSSYRDVGQRAG
Rv1058|M.tuberculosis_H37Rv MYGTMQDFPLTITAIMRHGCGVHGRRTVTTATGEG-YRHSSYRDVGQRAG
MMAR_4412|M.marinum_M MYSTMQDFPLTITAIMRHGCGVHGSRQVITATGDA-YRQTTYREVGQRAA
MUL_0229|M.ulcerans_Agy99 MYSTMQDFPLTITAIMRHGCGVHGSRQVITATGDA-YRQTTYREVGQRAA
MAV_1186|M.avium_104 MDGTMQDFPLTITAIMRHGCSVHGARTVTTATGDG-YRRTSYRELGDRAA
TH_2961|M.thermoresistible__bu MQSTMQPIPLTVAAIMRYAIEVHGDRTVTTATGDGGYRHATYADLGREAA
MAB_2080|M.abscessus_ATCC_1997 ----MQNVPLTVSAIVQHAAAIHGDSEVVTPTGNG-YRRTPYRIVLARVA
Mflv_1585|M.gilvum_PYR-GCK MLGLMQDRPLMISSLIEHAAAFHGDTEIVSRLPEGPVRRTTWREIGERAK
MLBr_01051|M.leprae_Br4923 --MLLASLNPSVVTATDIADAVCVDGVVLSRSG-----------------
: : : :
MSMEG_5348|M.smegmatis_MC2_155 RLAGVLDALAVPVGARVGSFGWNTQRHLELYLAVPCTNRVLHTVNHRLFA
Mvan_0905|M.vanbaalenii_PYR-1 RLATVFDELGISSDARVATFCWNTEEHLALYLAAPCTGRVLHTVNIRLFP
Mb1087|M.bovis_AF2122/97 QLANALRRLGVTGDQRVATFMWNNTEHLVTYFAVPSMGAVLHTLNIRLFP
Rv1058|M.tuberculosis_H37Rv QLANALRRLGVTGDQRVATFMWNNTEHLVTYFAVPSMGAVLHTLNIRLFP
MMAR_4412|M.marinum_M QLANALRGLGISGDQRVATFMWNNAEHLVAYLAVPSMGAVLHTLNIRLFA
MUL_0229|M.ulcerans_Agy99 QLANALRGLGISGDQRVATFMWNNAEHLVTYLAVPSMGAVLHTLNIRLFA
MAV_1186|M.avium_104 QLANALRGLGVTGDQRVATFMWNNAEHLAAYLAVPSMGAVLHTLNIRLFP
TH_2961|M.thermoresistible__bu RLANALRRLGITGDERVATFQWNNQEHLATYVAVPSMGAVLHTLNIRLFP
MAB_2080|M.abscessus_ATCC_1997 RLANALRSLGVTADQRVATFQWSNQEHLEAYCAIPSMGAVLHTLNIRLAP
Mflv_1585|M.gilvum_PYR-GCK QVANALAELGVRHGDRVATLAWNSDRHLALYFGVSGTGAVMHTVNPRLFP
MLBr_01051|M.leprae_Br4923 -----LVGAATSVAERVG------GARLVAVLATPTASTVLAITGCLIAG
: . **. :* . . . *: . :
MSMEG_5348|M.smegmatis_MC2_155 DDIVYIVNDAADDVLFVDRSLLDVVWPLVGRFTTVRHVIVMNDG-SGPQI
Mvan_0905|M.vanbaalenii_PYR-1 EQIAYVVNHARDEIIFVERSLLSVLWPLADQLPEVRYFVVIDDG-TDHEI
Mb1087|M.bovis_AF2122/97 EQIAYVTNEAEDRVILVDLSLARLLAPVLPKLDTVHTVIAVGEGDTTPLR
Rv1058|M.tuberculosis_H37Rv EQIAYVTNEAEDRVILVDLSLARLLAPVLPKLDTVHTVIAVGEGDTTPLR
MMAR_4412|M.marinum_M EQIAYVANEAEDQVVLVDLSLTNLLAPVLPDLGTVHTVIAVGEGDTAPLE
MUL_0229|M.ulcerans_Agy99 EQIAYVANEAEDQVVLVDLSLTNLLAPVLPNLDTVHTVIAVGEGDTAALE
MAV_1186|M.avium_104 EQIAYVANEAGDQVVLVDASLVKLLAPVLPGLHTVHTVIVVGDGDTEPLR
TH_2961|M.thermoresistible__bu EQLQFIAYEAQDRVVIVDASLVPLLAPVLPTMETVHTVIVVGAGDLSPLQ
MAB_2080|M.abscessus_ATCC_1997 EQLAYIANHASDQIVLVDASVAPLLARALPAMASVHTVIVTGEGDLAPLQ
Mflv_1585|M.gilvum_PYR-GCK EQISYIVNHAEDRVLFFDITFAPLVSGLASSFDSVEYFVAMTDREHMPDV
MLBr_01051|M.leprae_Br4923 VPVVPVPADIG-------------VVERRHMLTDSGAQAWLGPGPSADSA
: : . : :
MSMEG_5348|M.smegmatis_MC2_155 P--DGA--HDYEHLLAEARPVA-RFEVSDERTAAALCYTSGTTGRPKGVL
Mvan_0905|M.vanbaalenii_PYR-1 P--DSARILSYSSLLADSTPFDGAFVVDDENTAAAMCYTSGTTGNPKGVV
Mb1087|M.bovis_AF2122/97 E--AGKTVLRFAELIDAE-SPDFGWPQIDENSAAAMCYTSGTTGNPKGVV
Rv1058|M.tuberculosis_H37Rv E--AGKTVLRFAELIDAE-SPDFGWPQIDENSAAAMCYTSGTTGNPKGVV
MMAR_4412|M.marinum_M E--SGKTVLRYDELLDGQ-PTEFDWPHIDENSAAAMCYTSGTTGHPKGVV
MUL_0229|M.ulcerans_Agy99 E--SGKTVLRYDELLDGQ-STEFDWPHIDENSAAAMCYTSGTTGHPKGVV
MAV_1186|M.avium_104 A--SGKTVLRYADVIGAE-PAEFDWPRIDENSAAAMCYTSGTTGNPKGVV
TH_2961|M.thermoresistible__bu V--PGKQVLRYEEILAAE-SDEFDWPELDENSAAAMCYTSGTTGHPKGVV
MAB_2080|M.abscessus_ATCC_1997 G--CGKTVLRYEEVLAGQ-EESFDWPRLDELSAAAMCYTSGTTGDPKGVV
Mflv_1585|M.gilvum_PYR-GCK PGISADRLLCWEDVIGRQ-STHYEWPEFDECSASSLCYTSGTTGNPKGVL
MLBr_01051|M.leprae_Br4923 P----DGLPHIPVQLDAR--SWNRYPEPSPDATAMVIYTSGTTGPPKGVL
: : . ::: : ******* ****:
MSMEG_5348|M.smegmatis_MC2_155 YDHRSIVLHAMSLLMADAFGIGEADVVMPIVPMFHVNAWGLPYAAVMSGA
Mvan_0905|M.vanbaalenii_PYR-1 YSHRSTVLHSLAALAADVLGLSERDVVMPVVPMFHANSWGLPYAALLTGA
Mb1087|M.bovis_AF2122/97 YSHRSSFLHTMAACTTNGIGVGSSDKVLPIVPMFHANGWGLPYAALMAGA
Rv1058|M.tuberculosis_H37Rv YSHRSSFLHTMAACTTNGIGVGSSDKVLPIVPMFHANGWGLPYAALMAGA
MMAR_4412|M.marinum_M YSHRSSFLHSMATCTANGIGVGSNDRVLPIVPMFHANAWGLPYAALMAGA
MUL_0229|M.ulcerans_Agy99 YSHRSSFLHSMATCTANGIGLGSSDRVLPIVPMFHANAWGLPYAALMAGA
MAV_1186|M.avium_104 YSHRSSFLHTMAACTANGIGIGATDSLLPIVPMFHANAWGLPYAALMAGA
TH_2961|M.thermoresistible__bu YSHRSSYLHSTAVCSGNGLGLGFEDRVLPIVPMFHANAWGLPYAALMAGA
MAB_2080|M.abscessus_ATCC_1997 YSHRSTYLHSLTACTANALSVSEADRVLAIVPMFHANAWGLIYAALMSGA
Mflv_1585|M.gilvum_PYR-GCK YSHRSTMLHTLMAAARDTIDIHSGSVILLVVPMFHANAWGSPYTAAMVGA
MLBr_01051|M.leprae_Br4923 LSRRAIAVD--LDALAQAWQWTADDVLVHGLPLFHVHGLVLGLLGSLRIG
.:*: :. : . :: :*:**.:. . : .
MSMEG_5348|M.smegmatis_MC2_155 SLVLPGPAMQPASLAETLDTAGVTFAAAVTTVWRNVLPHLS---GRSLPS
Mvan_0905|M.vanbaalenii_PYR-1 GVVLPGPNMSPKALLGLLADHRVTVTAGVPTIWMGAAPLLA---DYDLSA
Mb1087|M.bovis_AF2122/97 DLVLPDRHLDARSLIHMVETLKPTLAGAVPTIWNDVMHYLEKDPDHDMSS
Rv1058|M.tuberculosis_H37Rv DLVLPDRHLDARSLIHMVETLKPTLAGAVPTIWNDVMHYLEKDPDHDMSS
MMAR_4412|M.marinum_M DLVLPDRHLDARSLVHMVENLRPTRAGAVPTIWNDVMHYLEKNPGHDVSS
MUL_0229|M.ulcerans_Agy99 DLVLPDRHLDARSLVHMVENLRPTRAGAVPTIWNDVMHYLEKNPGHDVSS
MAV_1186|M.avium_104 DLVLPDCHLDPRSLVRMVEDLRPTVTGAVPTIWNAVLHHLEDDPDHDMSS
TH_2961|M.thermoresistible__bu DLVMPDRYLDAKSLVNLIETQRPTVAGAVPTIWNDVLHHLDSEPDHDISS
MAB_2080|M.abscessus_ATCC_1997 DLVLPDRYLQAEPLVSIIEDTRPTVAGAVPTIWNDVDRYLDSHPERDISS
Mflv_1585|M.gilvum_PYR-GCK KLVMPGPHLDGESVYQLMKAEGVNQSQGVPTVWMMLFAYLDEHPEIDAHE
MLBr_01051|M.leprae_Br4923 NRFVHTGKPTPTAYAQACSEAGGSLYFGVPTVWSRLVADEAVARALRPAR
.: . . .*.*:*
MSMEG_5348|M.smegmatis_MC2_155 VRR--LVSGGGPLPESLSRRYASEIGVPLCSSWGMTETSPLVCSARIPTG
Mvan_0905|M.vanbaalenii_PYR-1 LRY--ILCGGSAVPKALSEEYRQAIGVSITQVWGMTETSPIAAACTPRTQ
Mb1087|M.bovis_AF2122/97 LRL--VACGGSAVPESLMRTFEDKHDVQIRQLWGMTETSPLATMAWPPPG
Rv1058|M.tuberculosis_H37Rv LRL--VACGGSAVPESLMRTFEDKHDVQIRQLWGMTETSPLATMAWPPPG
MMAR_4412|M.marinum_M LGL--VACGGSAVPESLMRTFEEKYGVQIRQLWGMTETSPLATMAWPPPG
MUL_0229|M.ulcerans_Agy99 LGL--VACGGSAVPESLMRTFEEKYGVQIRQLWGMTETSPLATMAWPPPG
MAV_1186|M.avium_104 LRL--VVCGGSAVPVSLMRTFEEKHGVQIRQLWGMTETSPLATMAWPPPG
TH_2961|M.thermoresistible__bu LRL--VGCGGSAVPVSLMQSFEEKFGVQIRQLWGMTETSPVCTLAWPAPN
MAB_2080|M.abscessus_ATCC_1997 LRL--VACGGSAVPLSLMRAFEEKYNVPIVQAWGMTETSPLATVARPAHR
Mflv_1585|M.gilvum_PYR-GCK LGLRYAGTGGAALPLSMIERFEKDFGAEAYQGWGMTETSPLCVVGRPLPK
MLBr_01051|M.leprae_Br4923 LLV----SGSASLPVPVFDRLAHLTGHRPIERYGSTES--LITLSTLADG
: *...:* .: . . :* **: :
MSMEG_5348|M.smegmatis_MC2_155 ----HSGDEVETLCLPGVSIPLVQLRLQR-DDGSFAPHDGKSSGELQVAG
Mvan_0905|M.vanbaalenii_PYR-1 YAELTTQEKAVALARQGQAVPLVDLRLVDPDTGADVPWDNHSTGEVQAAG
Mb1087|M.bovis_AF2122/97 TPDDQ---HWAFRITQGQPVCGVETRIVD-DDGQVLPNDGNAVGEVEVRG
Rv1058|M.tuberculosis_H37Rv TPDDQ---HWAFRITQGQPVCGVETRIVD-DDGQVLPNDGNAVGEVEVRG
MMAR_4412|M.marinum_M TPPDR---HWAIRSTQGQPVCGVETRIVD-DNGEALPNDGQAVGEVEVRG
MUL_0229|M.ulcerans_Agy99 TPPDR---HWAIRSTQGQPVCGVETRIVD-DNGEALPNDGQAVGEVEVRG
MAV_1186|M.avium_104 TPEDQ---HWAYRGTQGQPVCGVQMRIVD-DDGRVLPNDGTAVGEVEVRG
TH_2961|M.thermoresistible__bu TPADR---HWEVRGSQGRPICGVEARIVD-DEGNVLPRDNEAVGELEVRG
MAB_2080|M.abscessus_ATCC_1997 ADATR---RWEMRASQGRPICGVEIRLRD-DDGKDVPWDGQSVGEIQARG
Mflv_1585|M.gilvum_PYR-GCK HDALDPADRRSLRLKQGRGIWGVEIKIVD-DDGNRLPWDGKAFGEVWVRG
MLBr_01051|M.leprae_Br4923 ERRAG---------WVGLPLAGVQTRLVD-ESGGPVPYDGETVGRLQVRS
* : *: :: : * * *. : *.: . .
MSMEG_5348|M.smegmatis_MC2_155 PTIASGYYGATDG-SAAFTEDGWLRTGDVATIDAHGLVRIVDR-IKDLIK
Mvan_0905|M.vanbaalenii_PYR-1 PWIASSYYNSDDQ-QSSFTDDGWLRTGDVGVCDEFGSLLLVDR-TKDLVK
Mb1087|M.bovis_AF2122/97 PWIAGSYYGGRD--ESKFDS-GWLRTGDVGRIDEQGFITLTDR-AKDVIK
Rv1058|M.tuberculosis_H37Rv PWIAGSYYGGRD--ESKFDS-GWLRTGDVGRIDEQGFITLTDR-AKDVIK
MMAR_4412|M.marinum_M PWITGAYYLGYD--QSKFDS-GWLRTGDVGRIDELGFITLTDR-AKDVIK
MUL_0229|M.ulcerans_Agy99 PWITGAYYLGYD--QSEFDS-GWLRTGDVGRIDELGFITLTDR-AKDVIK
MAV_1186|M.avium_104 PWIAGSYYLGRD--DSKFDS-GWLRTGDVGRIDERGFVTLTDR-AKDVIK
TH_2961|M.thermoresistible__bu PWITGSYYHDAD--PSKFEN-GWLRTGDVGRMDAQSYITLTDR-AKDVIK
MAB_2080|M.abscessus_ATCC_1997 PWITGSYFGDND--TEKFDE-GWLRTGDVGKIDAEGYLTLTDR-SKDVIK
Mflv_1585|M.gilvum_PYR-GCK PWIASGYYRGEG--GDKLDGEGFFPTGDVATIDPDGYLQLVDR-TKDVIK
MLBr_01051|M.leprae_Br4923 PTMFGGYLNRPEATAEAFDEDGWYRTGDVAVVDSGGMHRIVGRESVDLIK
* : ..* : *: ****. * . :..* *::*
MSMEG_5348|M.smegmatis_MC2_155 SGGEWISSVELENAIMTCPAVAEAAVVGAPHERWGERPLAFVVPAAGHDA
Mvan_0905|M.vanbaalenii_PYR-1 SGGEWISSVQLENEIMAHPKVSEAAVIAIPHERWVERPLACVVVKQGESM
Mb1087|M.bovis_AF2122/97 SGGEWISSVELENCLIAHPDVLEAAVVGVPDERWQERPLAVVVVREGATV
Rv1058|M.tuberculosis_H37Rv SGGEWISSVELENCLIAHPDVLEAAVVGVPDERWQERPLAVVVVREGATV
MMAR_4412|M.marinum_M SGGEWISSVELENCLIAHPDVVEAAVVGVPDERWQERPLAVVVRREGATV
MUL_0229|M.ulcerans_Agy99 SGGEWISSVELENCLIAHPDVVEAAVVGVPDERWQERPLAVVVRREGATV
MAV_1186|M.avium_104 SGGEWISSVELENCLIGHPDVVEAAVVGVPDERWEERPLAVVVVKDGASV
TH_2961|M.thermoresistible__bu SGGEWISSVELENHLIGHPKVREAAVVGVPDDRWQERPLAAVVLHEGQQA
MAB_2080|M.abscessus_ATCC_1997 SGGEWISSVELENTLIGHPAVYEAAVVGVADDKWQERPLALVVVHPGTDV
Mflv_1585|M.gilvum_PYR-GCK SGGEWVSSIDLENAAMGHPAIAEAAVIGVPHPKWQERPLLVAVLRKGHSA
MLBr_01051|M.leprae_Br4923 LGGYRIGAGEIEMALLGHPDVREVAVVGLPDEDLGQRIVAFVVG--AEAL
** :.: ::* : * : *.**:. .. :* : .* .
MSMEG_5348|M.smegmatis_MC2_155 TPDLVRDHLQQRVARWWIPDDIVIVDELPKTATGKISKRALR---QNLCA
Mvan_0905|M.vanbaalenii_PYR-1 TTEEVIRHLSARVAKWWLPDAVEFIDAVPKTSVGKFSKKTLRARFENYRF
Mb1087|M.bovis_AF2122/97 SAGDLRAFLADKVVRWWLPERWAFVDEIPRTSVGKYDKKAIRSRYAEGAY
Rv1058|M.tuberculosis_H37Rv SAGDLRAFLADKVVRWWLPERWAFVDEIPRTSVGKYDKKAIRSRYAEGAY
MMAR_4412|M.marinum_M SAAELRKFLADRVVRWWLPERWAFADQVPRTSVGKYDKKTIRAGYAEGVY
MUL_0229|M.ulcerans_Agy99 SAAELRKFLADRVVRWWLPERWAFADQVPRTSVGKYDKKTIRAGYAESVY
MAV_1186|M.avium_104 DADQLRKFLADKVVRWWLPERWTFVDEIPRTSVGKYDKKAVRSRYADGGY
TH_2961|M.thermoresistible__bu TPEELREFLSDKVVRWWLPERWTFVEEIPRTSVGKYDKKTIRARYADGAY
MAB_2080|M.abscessus_ATCC_1997 DIDQLRSFLADKVAKWWIPERWSFVSDIPRTSVGKYDKKAIRARHSAGEY
Mflv_1585|M.gilvum_PYR-GCK TREDILEFLAERVVRWWLPDDVVFVDELPHTATGKVLKITLREQYRDHEL
MLBr_01051|M.leprae_Br4923 DADELINYVAQQLSIHKRPREVRFVDALPRNAMGKVLKKQLLFEG-----
: .: :: * : . :*:.: ** * :
MSMEG_5348|M.smegmatis_MC2_155 PAG----
Mvan_0905|M.vanbaalenii_PYR-1 PDQGAPV
Mb1087|M.bovis_AF2122/97 QITEVHT
Rv1058|M.tuberculosis_H37Rv QITEVHT
MMAR_4412|M.marinum_M QVTEVHN
MUL_0229|M.ulcerans_Agy99 QVTDVHN
MAV_1186|M.avium_104 RVIEARD
TH_2961|M.thermoresistible__bu QVTHLGG
MAB_2080|M.abscessus_ATCC_1997 LIEIETS
Mflv_1585|M.gilvum_PYR-GCK SQPE---
MLBr_01051|M.leprae_Br4923 -------