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AMKLRPSRPVGHHLAPLAAAVGAVASENPAGSSSDLAAVHVTGVTLRGQDAQPGDLFAALPGASAHGARF AADAVAAGAVAVLTDPSGADLLAGTLDVPVLVHPAPRSVLGEAAAIVYGRPAERLRVVGVTGTSGKTTTT YLAEAGLRAAGRVAGLIGTVGVRIDGTDLPSALTTPEAPALQALLALMVERGVDTVVMEVSSHALAQGRV DGIGFAVGGFTNLSRDHLDFHPTMADYFEAKARLFDPQSPNHAAAAVICVDDQYGVQMAARAETPLTVST SGAAADWRVEKITTAGPGAQEFVLVDPGGMHHQLGVGLPGRYNVANAALAVALLDAVGVSPEQAAPGLRT ATVPGRLESIDLGQDFLALVDYAHKPGALQAVLETLRAAEPRRIAVVFGAGGDRDPGKREPMGRVTAELA DLVVVTDDNPRSEDPATIRSAILAGARAVASDAEIVEIGDRRAAIDHAVAWAQPGDIVLIAGKGHESGQT VGDHTRPFDDRDELAAALEASGKTRDRSS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_4232 | murE | - | 100% (520) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase |
| M. smegmatis MC2 155 | MSMEG_4231 | - | 2e-18 | 25.59% (512) | UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase |
| M. smegmatis MC2 155 | MSMEG_4229 | murD | 6e-14 | 26.37% (531) | UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb2182c | murE | 0.0 | 68.84% (507) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. gilvum PYR-GCK | Mflv_2983 | murE | 0.0 | 73.33% (510) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. tuberculosis H37Rv | Rv2158c | murE | 0.0 | 68.84% (507) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. leprae Br4923 | MLBr_00909 | murE | 0.0 | 64.24% (509) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. abscessus ATCC 19977 | MAB_2001 | murE | 1e-172 | 66.26% (486) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. marinum M | MMAR_3198 | murE | 0.0 | 67.84% (513) | UDP-N-acetylmuramoylalanyl-D-glutamate-2,6- diaminopimelate |
| M. avium 104 | MAV_2331 | murE | 0.0 | 69.14% (512) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. thermoresistible (build 8) | TH_2285 | - | 2e-10 | 26.72% (393) | POSSIBLE LIGASE |
| M. ulcerans Agy99 | MUL_3506 | murE | 0.0 | 67.06% (513) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
| M. vanbaalenii PYR-1 | Mvan_3528 | murE | 0.0 | 74.45% (501) | UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2983|M.gilvum_PYR-GCK ----------------------------------------------MNLR
Mvan_3528|M.vanbaalenii_PYR-1 --------------------------------------------------
MSMEG_4232|M.smegmatis_MC2_155 --------------------------------------------MAMKLR
Mb2182c|M.bovis_AF2122/97 --------------------MSSLARGISR---RRTEVATQVEAAPTGLR
Rv2158c|M.tuberculosis_H37Rv --------------------MSSLARGISR---RRTEVATQVEAAPTGLR
MMAR_3198|M.marinum_M --------------------------------------MSEVESV---LR
MUL_3506|M.ulcerans_Agy99 MGSRRGAGWVRHRVVTGRDWVGCHGRPVSPGVGRRTEVVSEVESV---LR
MAV_2331|M.avium_104 -----------------------------------------MVSVPTGLR
MLBr_00909|M.leprae_Br4923 --------------------------------------MTEVVAVPVRLR
MAB_2001|M.abscessus_ATCC_1997 -----------------------------------------MAASGTHRF
TH_2285|M.thermoresistible__bu --------------------------------------------------
Mflv_2983|M.gilvum_PYR-GCK PSHPVGMPLGALADLVCAEPAD-----AGPVPDLQVTGVTLRGQHAQAGD
Mvan_3528|M.vanbaalenii_PYR-1 ----------MLADLVAAVAAG-----GGGAPDVQVTGVTLRGQDAQPGD
MSMEG_4232|M.smegmatis_MC2_155 PSRPVGHHLAPLAAAVGAVASENPAGSSSDLAAVHVTGVTLRGQDAQPGD
Mb2182c|M.bovis_AF2122/97 PNAVVGVRLAALADQVGAALAEG-PAQRAVTEDRTVTGVTLRAQDVSPGD
Rv2158c|M.tuberculosis_H37Rv PNAVVGVRLAALADQVGAALAEG-PAQRAVTEDRTVTGVTLRAQDVSPGD
MMAR_3198|M.marinum_M PSAGPGVPLAELAVQVGAVLADG-PHRAAAVPDTLITGMTLRAQAVQPGD
MUL_3506|M.ulcerans_Agy99 PSAGPGVPLAELAVQVGAVLADG-PHRAAAVPDTLITGMTLRAQAVQPGD
MAV_2331|M.avium_104 PSAVPGVRLPALADQVGAVMAG--PDRRAAVPDVTVTGVTLRAQDVLPGD
MLBr_00909|M.leprae_Br4923 PSATAGVRLPELVAQVGAVLADG-PGQAATVPDIPVTGVTLRAQEVLSGD
MAB_2001|M.abscessus_ATCC_1997 LPRTHQHLVSDLASLVSAELHGP--------SATTVGGVTLRAQDARTGD
TH_2285|M.thermoresistible__bu -------------------------------------MVTLRGR------
:***.:
Mflv_2983|M.gilvum_PYR-GCK LFAALPGSKAHGARYAADAVERGAVAVLTDADGVSEIAAH--GGPAVPIL
Mvan_3528|M.vanbaalenii_PYR-1 LFAALPGSRAHGGRYAAEAVGRGAVAVLTDAAGEAEIASR--GAPDVPVL
MSMEG_4232|M.smegmatis_MC2_155 LFAALPGASAHGARFAADAVAAGAVAVLTDPSGADLLA----GTLDVPVL
Mb2182c|M.bovis_AF2122/97 LFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGR----AAVPVL
Rv2158c|M.tuberculosis_H37Rv LFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGR----AAVPVL
MMAR_3198|M.marinum_M LFAALAGSATHGARHAADAIERGAVAVLTDPAGVTAISALGCVPGTVPVL
MUL_3506|M.ulcerans_Agy99 LFAALAGSATHGARHTADAIERGAVAVLTDPAGVTAISALGCVPGTVPVL
MAV_2331|M.avium_104 LFAALPGATTHGARHAAEAIERGAVAVLTDAAGVAQLTGG----RTTPTL
MLBr_00909|M.leprae_Br4923 LFAALAGASTHGARYAGVALERGAVAVLTDVAGVAELTAQ---ASSVPIL
MAB_2001|M.abscessus_ATCC_1997 LFAALPGTSTHGAQFASEAVSAGAAAVLTDAAGAELVAGQG---LDIAVL
TH_2285|M.thermoresistible__bu ----LALAAGTAARWASRATGRGEGAMIGG------------------LI
*. : ..: .. * * *:: . :
Mflv_2983|M.gilvum_PYR-GCK VHPAPRSVLGEVAAAVFGRPSEHLRVIGVTGTSGKTTTTYLVEAGLRSAG
Mvan_3528|M.vanbaalenii_PYR-1 VHPDPRAVLGELASAVYGRPSQHLRVIGVTGTSGKTTTTYLVEAGLRAAG
MSMEG_4232|M.smegmatis_MC2_155 VHPAPRSVLGEAAAIVYGRPAERLRVVGVTGTSGKTTTTYLAEAGLRAAG
Mb2182c|M.bovis_AF2122/97 VHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAG
Rv2158c|M.tuberculosis_H37Rv VHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAG
MMAR_3198|M.marinum_M VHPAPRGVLGEAAALVYGHPSDRLTVVGITGTSGKTTTTYLVEAGLRAAG
MUL_3506|M.ulcerans_Agy99 VHPAPRGVLGEAAALVYGHPSDRLTVVGITGTSGKTTTTYLVEAGLRAAG
MAV_2331|M.avium_104 LHPRPRSVLGELAAAVYAHPSERLTVIGITGTSGKTTTTYLVESGLRAAG
MLBr_00909|M.leprae_Br4923 IHPEPRSVLGGLAAAAYGHPSNRMTVVGITGTSGKTTTTYMVEAGLRAGG
MAB_2001|M.abscessus_ATCC_1997 VHPVPRSVLGELAATVYGEPSRHLQVVGVTGTSGKTTTTYLVEAGLRAAG
TH_2285|M.thermoresistible__bu AMTLDRSILRQLG--------QGRRTALVTGTNGKSTTTRMLAAALGTVG
. *.:* . . :***.**:*** : :.* : *
Mflv_2983|M.gilvum_PYR-GCK RVAGLIGTVGVRIDGRAQPGGLTTPEAPDLQALLAAMLEQGVDTVVMEVS
Mvan_3528|M.vanbaalenii_PYR-1 RVAGLIGTVGVRIDGRQQPGGLTTPEAPELQALLATMLEQGVDTVVMEVS
MSMEG_4232|M.smegmatis_MC2_155 RVAGLIGTVGVRIDGTDLPSALTTPEAPALQALLALMVERGVDTVVMEVS
Mb2182c|M.bovis_AF2122/97 RVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVS
Rv2158c|M.tuberculosis_H37Rv RVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVS
MMAR_3198|M.marinum_M RVAGLIGTIGIRIDGADIPSTLTTPEAPALQAMLAAMVERGVDTVVMEVS
MUL_3506|M.ulcerans_Agy99 RVAGLIGTIGIRIDGADIPSTLTTPEAPTLQAMLAAMVERGVDTVVMEVS
MAV_2331|M.avium_104 RTAGLIGTVGIRIDGADIPSALTTPEAPALQALLAAMAEQRVDTVVMEVS
MLBr_00909|M.leprae_Br4923 RVVGLVGTIGIRIDGADIPSFLTTPEAPALQAMLAAMVERGVETVVMEVS
MAB_2001|M.abscessus_ATCC_1997 KRVGLIGTVGVRIDGEDVPSALTTPEAPALQELLAEMVQRGVDTVVMEVS
TH_2285|M.thermoresistible__bu GVA--TNAEGANMDAGLVSALAVNRDAGLAALEVDEMHTPQVADALRPAV
. .: * .:.. .. .. :* : * * .: .
Mflv_2983|M.gilvum_PYR-GCK SHALTLGRVDGVSF-AVGGFTNLSRDHLDFHPTMQDYFEAKARLVDPTSP
Mvan_3528|M.vanbaalenii_PYR-1 SHALTLGRVDGVSF-AVGGFTNLSRDHLDFHPTMEDYFDAKARLFDPACA
MSMEG_4232|M.smegmatis_MC2_155 SHALAQGRVDGIGF-AVGGFTNLSRDHLDFHPTMADYFEAKARLFDPQSP
Mb2182c|M.bovis_AF2122/97 SHALALGRVDGTRF-AVGAFTNLSRDHLDFHPSMADYFEAKASLFDPDSA
Rv2158c|M.tuberculosis_H37Rv SHALALGRVDGTRF-AVGAFTNLSRDHLDFHPSMADYFEAKASLFDPDSA
MMAR_3198|M.marinum_M SHALTLGRVDGIRF-AVGGFTNLSRDHLDFHPTMADYFEAKALLFDPASA
MUL_3506|M.ulcerans_Agy99 SHALTLGRVDGIRF-AVGGFTNLSRDHLDFHPTMADYFEAKALLFDPASA
MAV_2331|M.avium_104 SHALALGRVDGTRF-AVGGFTNLSRDHLDFHPTMADYFEAKALLFDPNSP
MLBr_00909|M.leprae_Br4923 SHALSLGRVEGTQF-AVAGFTNLSRDHLDFHPDMEEYFEAKAVLFDPHSL
MAB_2001|M.abscessus_ATCC_1997 SHALALNRVDGTRF-AVGAFTNLSRDHLDFHPDMQDYFATKARLFRRDSP
TH_2285|M.thermoresistible__bu VVLLNLSRDQLDRVGEINHIERTLRAGLARHPDAVVVANCDDVLMTSAAY
* .* : . : : . * * ** . *. .
Mflv_2983|M.gilvum_PYR-GCK THAARSVICVDDEWGRAMAARADAP----VTVS-VTG-DADWWVQEVRAL
Mvan_3528|M.vanbaalenii_PYR-1 THAARSVICVDDEWGRAMAARARRP----VTVS-TTG-PADWRAEQVRSA
MSMEG_4232|M.smegmatis_MC2_155 NHAAAAVICVDDQYGVQMAARAETP----LTVS-TSGAAADWRVEKITTA
Mb2182c|M.bovis_AF2122/97 LRARTAVVCIDDDAGRAMAARAADA----ITVS-AADRPAHWRATDVAPT
Rv2158c|M.tuberculosis_H37Rv LRARTAVVCIDDDAGRAMAARAADA----ITVS-AADRPAHWRATDVAPT
MMAR_3198|M.marinum_M LRGHSVVVCIDDEAGRAMADRAGAA----ITVS-ATGQPAHWRATDVAPV
MUL_3506|M.ulcerans_Agy99 LRGHSVVVCIDDEAGRAMADRAGVA----ITVS-ATGQPAHWRATDVAPV
MAV_2331|M.avium_104 LRAHRAVVCIDDEAGREMAARAGDA----VTVS-AEGQPAHWRAVEVAPL
MLBr_00909|M.leprae_Br4923 LRARTVVVCIDDDAGRATAARAGDA----ITVS-ALGQPASWRATDIRSP
MAB_2001|M.abscessus_ATCC_1997 QRAHRSVVCIDDDAGRAMAELAREAGPDVTTVSSLDGGVADWRCSNVAVG
TH_2285|M.thermoresistible__bu DSPNVVWVAAGAGWANDSVSCPRSGE---VIVRDGAHWYSTGTDFKRPTP
:. . . . . * : .
Mflv_2983|M.gilvum_PYR-GCK DGGVQDFLAVDPAGVHHALEIGLPGGYNVANCLLAVALLDAVDVSPEQAA
Mvan_3528|M.vanbaalenii_PYR-1 GGGAQDFVAVDPAGVHHGLEIGLPGGYNVANCLLAIALLDAVGVSPEQAA
MSMEG_4232|M.smegmatis_MC2_155 GPGAQEFVLVDPGGMHHQLGVGLPGRYNVANAALAVALLDAVGVSPEQAA
Mb2182c|M.bovis_AF2122/97 DAGGQQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAV
Rv2158c|M.tuberculosis_H37Rv DAGGQQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAV
MMAR_3198|M.marinum_M GAGGQEFTALDPAGVGHRVRIGLPGHYNVANCLLALAILDAVGVSPEQAA
MUL_3506|M.ulcerans_Agy99 GAGGQEFTALDPAGVGHRVRIGSPGHYNVSNCLLALAILDAVGVSPEQAA
MAV_2331|M.avium_104 GTGGQQFTVIDPAGVQHRAGIRLPGHYNVANCLVALALLDAVGVSPEQAA
MLBr_00909|M.leprae_Br4923 GVGAQEFTAVDPAGVQHRVGIRLPGRYNVANCLVALAILDVVGVSPEQAS
MAB_2001|M.abscessus_ATCC_1997 HDGGQRFVANGP-DRDTELTIALPGHYNVANCLVAIAILDVLGVSAATAA
TH_2285|M.thermoresistible__bu QWWFDDTSIHGPDGLCLPMRLALPGVVNRGNATQAVAAAVAMGADPGAAV
: .* . : ** * .*. *:* .:.... *
Mflv_2983|M.gilvum_PYR-GCK PGLR-DATVPGRLEAVDRGQPFLALVDYAHKPGALAAVLQTLREQCAGRL
Mvan_3528|M.vanbaalenii_PYR-1 PGVR-DATVPGRLESVDRGQPFLALVDYAHKPGALRAVLQTLREQCTGRL
MSMEG_4232|M.smegmatis_MC2_155 PGLR-TATVPGRLESIDLGQDFLALVDYAHKPGALQAVLETLRAAEPRRI
Mb2182c|M.bovis_AF2122/97 PGLR-EIRVPGRLEQIDRGQGFLALVDYAHKPEALRSVLTTLAHP-DRRL
Rv2158c|M.tuberculosis_H37Rv PGLR-EIRVPGRLEQIDRGQGFLALVDYAHKPEALRSVLTTLAHP-DRRL
MMAR_3198|M.marinum_M PGLR-ETRVPGRLEQVDRGQDFLALVDYAHKPGALREVLSTLRNP-QRRL
MUL_3506|M.ulcerans_Agy99 PGLR-ETRVPGRLEQVDRGQDFLALVDYAHKPGALREVLSTLRNP-QRRL
MAV_2331|M.avium_104 PGLL-QTRVPGRMEEIDAGQDFLALVDYAHKPGALRAVLSSLKRP-DRRL
MLBr_00909|M.leprae_Br4923 LGFR-DIRIPGRLERIDCGQDFLALVDYAHKPGALHSVLTALLQP-DHRL
MAB_2001|M.abscessus_ATCC_1997 PGIA-TAGVPGRMEAVAAGQPFLAVVDYAHKPGALRAVLESLRSHRDGRL
TH_2285|M.thermoresistible__bu SAVSGVDEVAGRYRTVSLG-DHTVRVLLAKNPAGWQESLSMVDKTAAGVV
.. :.** . : * . . * *::* . * : :
Mflv_2983|M.gilvum_PYR-GCK AVVFGAGGDRDQGKREPMGRVAAELADLVVVTDDNPRGEDPAVIRAAILR
Mvan_3528|M.vanbaalenii_PYR-1 AVVFGAGGNRDQGKREPMGRVAAELADLVVVTDDNPRDEDPAVIRAAILA
MSMEG_4232|M.smegmatis_MC2_155 AVVFGAGGDRDPGKREPMGRVTAELADLVVVTDDNPRSEDPATIRSAILA
Mb2182c|M.bovis_AF2122/97 AVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILA
Rv2158c|M.tuberculosis_H37Rv AVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILA
MMAR_3198|M.marinum_M AVVFGAGGDRDPGKRGPMGRIAAELADLVVVTDDNPRGEDPGAIRRTIVA
MUL_3506|M.ulcerans_Agy99 AVVFGAGGDRDPGKRGPMGRIAAELADLVVVTDDNPRGEDPGAIRRTIVA
MAV_2331|M.avium_104 AVVFGAGGERDPGKRAPMGATAAELADLVVVTDDNPRGEDPAAIRREILA
MLBr_00909|M.leprae_Br4923 AVVFGAGGERDPGKRAPMGEIAAELADLVVVTDDNPRGEDPAAIRRDILT
MAB_2001|M.abscessus_ATCC_1997 AVVFGAGGNRDRGKRADMGKVAAELADLVVVTDDNPRNEEPAAIRTEIIS
TH_2285|M.thermoresistible__bu IAVNGQVPDGE--DLSWLWDVRFEHFERTSVVAAGERGTDLAVR------
.* * : : . : : : . *. . *. : .
Mflv_2983|M.gilvum_PYR-GCK GASG----AQTVEIGDRRAAIVHAVQWAREGDIVLIAGKGHEAGQTSQGR
Mvan_3528|M.vanbaalenii_PYR-1 GAAGAR--AEVVEIGDRRAAIGHAAGWARAGDIVLIAGKGHEAGQTSGGQ
MSMEG_4232|M.smegmatis_MC2_155 GARAVASDAEIVEIGDRRAAIDHAVAWAQPGDIVLIAGKGHESGQTVGDH
Mb2182c|M.bovis_AF2122/97 GAAEVGGDAQVVEIADRRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGR
Rv2158c|M.tuberculosis_H37Rv GAAEVGGDAQVVEIADRRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGR
MMAR_3198|M.marinum_M GAAESGGAAQVVEIADRGEAIGYAVDWARTGDVVLIAGKGHETGQRGGAE
MUL_3506|M.ulcerans_Agy99 GAAESGGAAQVVEIADRGEAIGYAVDWARTGDVVLIAGKGHETGQRGGAE
MAV_2331|M.avium_104 GATESGCAAEVIEIGDRRAAIRHAVAWAGPGDVVLVAGKGHETGQRTGEH
MLBr_00909|M.leprae_Br4923 GTVAAGGAAQVVEIGDRRAAIQYAVAWAGPDDVVLVAGKGHETGQRGAAE
MAB_2001|M.abscessus_ATCC_1997 GTTGS--EAQIIEIGDRRAAIDFATLWARPGDTVLVAGKGHESGQKTGDV
TH_2285|M.thermoresistible__bu -----------LTYADVAHTLVHDTVAAIKS-----CPPGHVEVIANYTA
: .* :: . . * . . **
Mflv_2983|M.gilvum_PYR-GCK TRPFDDRDELAAAL-ETLQTSGTSGASQ---
Mvan_3528|M.vanbaalenii_PYR-1 TRPFDDRDELAAAL-EARGARGAGG------
MSMEG_4232|M.smegmatis_MC2_155 TRPFDDRDELAAAL-EASGKTRDRSS-----
Mb2182c|M.bovis_AF2122/97 VRPFDDRVELAAAL-EALERRA---------
Rv2158c|M.tuberculosis_H37Rv VRPFDDRVELAAAL-EALERRA---------
MMAR_3198|M.marinum_M VRPFDDRVELARAL-EALESCT---------
MUL_3506|M.ulcerans_Agy99 VRPFDDRVELARAL-EALESCT---------
MAV_2331|M.avium_104 TRPFDDRVELAEALREAVGPRKAQG------
MLBr_00909|M.leprae_Br4923 TCPFDDRVELARAL-QVRDARLLPAPGRACQ
MAB_2001|M.abscessus_ATCC_1997 TVPFDDRVELRAAL-EALGSLDD--------
TH_2285|M.thermoresistible__bu FLHLNRRLS----------------------
:: * .