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TETADTTGLTGFFRDHDIHSLAADLRNGHTTSVELVEHSLAAITRLDPTLNAFTAVDAEGALDAARHADA ELARGHDRGVLHGIPVAVKDLVDVAGHVTTRGSVVHPGPATADAECVRRLRTAGAVIVGRNVLHEFAFGA TGDRSAHGASRNPWDPSRISGGSSGGSAVATAAGMVPLAVGTDTAGSVRVPAALCGVVGFKPAYGAIPAQ GVWPLAASLDHVGVFARTATGAAAAYTAMAGRSPDPVRAQRVAWLDPACLGPCDPAIIAALSETLRAAQI TVDGTVGLPFAAGEVFEALSVLQSSEAYTEYSAETEQRASDIDPEVLGRLLRGRDTPAWQYVRACRQRDR LRAAADTLLTRFDVLAMPAAPTVATEIDQRAHQIDGHAVEVRSALLSLTCPWNLTGHPALSVPAGVVSGL PVGLQLISAPGNEGMLFDLAARIEHR*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2986 | - | - | 100% (447) | amidohydrolase, AtzE family protein |
| M. smegmatis MC2 155 | MSMEG_1090 | - | 7e-61 | 36.50% (452) | amidase |
| M. smegmatis MC2 155 | MSMEG_1088 | gatA | 3e-60 | 35.81% (472) | glutamyl-tRNA(Gln)/aspartyl-tRNA(Asn) amidotransferase, A |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb3409 | amiD | 2e-53 | 33.79% (441) | amidase AmiD |
| M. gilvum PYR-GCK | Mflv_2712 | - | 3e-53 | 37.95% (448) | amidase |
| M. tuberculosis H37Rv | Rv3375 | amiD | 2e-53 | 33.79% (441) | amidase AmiD |
| M. leprae Br4923 | MLBr_01702 | gatA | 1e-47 | 32.23% (484) | aspartyl/glutamyl-tRNA amidotransferase subunit A |
| M. abscessus ATCC 19977 | MAB_1674c | - | 3e-50 | 34.59% (451) | amidase |
| M. marinum M | MMAR_3673 | amiA2 | 7e-50 | 36.56% (454) | amidase AmiA2 |
| M. avium 104 | MAV_2032 | - | 9e-51 | 36.28% (441) | amidase |
| M. thermoresistible (build 8) | TH_4482 | - | 7e-54 | 37.09% (453) | PUTATIVE Amidase |
| M. ulcerans Agy99 | MUL_3616 | amiA2 | 5e-50 | 36.34% (454) | amidase |
| M. vanbaalenii PYR-1 | Mvan_3824 | - | 3e-53 | 36.69% (447) | amidase |
CLUSTAL 2.0.9 multiple sequence alignment
Mflv_2712|M.gilvum_PYR-GCK -MPDSS-ARSTSRNRFPDRFPTVANQLFQLASGTATSVDLVRRSLVAIEA
Mvan_3824|M.vanbaalenii_PYR-1 -MADTAYSRITSRR----RFPTLGNQLFQLASGTATSVDLVSRSLQAIEA
TH_4482|M.thermoresistible__bu -MVDAYKPPAK--------LPTLTDLLYQLASGAVTSQELVRRSLRAIEA
MMAR_3673|M.marinum_M MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
MUL_3616|M.ulcerans_Agy99 MVGASGFASGAVSGSGGPRLPTLTDLLYQLASGSVTSVELTRRSLREIEV
MAV_2032|M.avium_104 ----------------------------------------MGRSLHAIHA
MAB_1674c|M.abscessus_ATCC_199 ----------------MSGFPTLTSQAAALSDGSESSVSLTLTALNAIKS
MSMEG_2986|M.smegmatis_MC2_155 ----MTETADTTGLTGFFRDHDIHSLAADLRNGHTTSVELVEHSLAAITR
Mb3409|M.bovis_AF2122/97 -MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIER
Rv3375|M.tuberculosis_H37Rv -MTDADSAVPPRLDEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIER
MLBr_01702|M.leprae_Br4923 ----------MIGSLHEIIRLDASTLAAKIVAKELSSVEITQACLDQIEA
* *
Mflv_2712|M.gilvum_PYR-GCK SQPTLNAFRVVLAERALADAAEADRLRAAGHDRARRPLLGIPIAVKDDVD
Mvan_3824|M.vanbaalenii_PYR-1 SQPTLNAFRVVLTEQALADAAEADRKRAAGADLSRHPLLGVPIAVKDDVD
TH_4482|M.thermoresistible__bu SQSTLNAFRVVLADRALAEAAEADRKRAAG---EHLPLLGVPIAVKDDVD
MMAR_3673|M.marinum_M SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDDVD
MUL_3616|M.ulcerans_Agy99 SQSTLNAFRVVLTESALADAAAADRRRAAG---DAAPLLGIPIAVKDDVD
MAV_2032|M.avium_104 SQPTLNAFRVVLTESALADAAEADRRRAAG---DTAPLLGIPIAVKDDVD
MAB_1674c|M.abscessus_ATCC_199 SQSTLNAFRVVCTKTALAEATEADKRLARG---ERLPLLGVPIAIKDDTD
MSMEG_2986|M.smegmatis_MC2_155 LDPTLNAFTAVDAEGALDAARHADAELARG--HDRGVLHGIPVAVKDLVD
Mb3409|M.bovis_AF2122/97 LDPQLKSYAFVMPETALAAARAADADIARG--HYEGVLHGVPIGVKDLCY
Rv3375|M.tuberculosis_H37Rv LDPQLKSYAFVMPETALAAARAADADIARG--HYEGVLHGVPIGVKDLCY
MLBr_01702|M.leprae_Br4923 TDDTYRAFLHVAAEKALSAAAAVDKAVAAG-GQLSSTLAGVPLALKDVFT
: .:: * .. ** * .* * * * *:*:.:**
Mflv_2712|M.gilvum_PYR-GCK VAGVPTRFGASGEVPE-AVADAEVVRRLRAAGAVIVGKTNTCELGQWPFT
Mvan_3824|M.vanbaalenii_PYR-1 VAGVPTRFGADGEVPP-AEADAEVVRRLRAAGAVIVGKTNTCELGQWPFT
TH_4482|M.thermoresistible__bu IAGVPTRFGAVGDVPP-ATEDAEVVRRLRAAGAVIVGKTHTCELGQWPFT
MMAR_3673|M.marinum_M VAGVPTAFGTQGYVRP-AAHDSEVVRRLKAAGAVIIGKTNTCELGQWPFT
MUL_3616|M.ulcerans_Agy99 VAGVPTAFGTQGYVQP-AAHDSEVVRRLKAAGAVIIGKTNTCELGQWPFT
MAV_2032|M.avium_104 IAGVPTAFGTEGSVPP-ATHDAEVVRRLKAAGAVIVGKTNTCELGQWPFT
MAB_1674c|M.abscessus_ATCC_199 LAGTPTAFGTAGAVGS-ESEDAELVRRLRAAGAVIVGKTNTCELGQWGFT
MSMEG_2986|M.smegmatis_MC2_155 VAGHVTTRGSVVHPGP-ATADAECVRRLRTAGAVIVGRNVLHEFAFGATG
Mb3409|M.bovis_AF2122/97 TVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYH
Rv3375|M.tuberculosis_H37Rv TVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVIIGKLAMTEGAYLGYH
MLBr_01702|M.leprae_Br4923 TVDMPTTCGSKILQGWHSPYDATVTTRLRAAGIPILGKTNMDEFAMGSST
.. * *: *: . **::** *:*: * .
Mflv_2712|M.gilvum_PYR-GCK SGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGSDGAGSVRIPA
Mvan_3824|M.vanbaalenii_PYR-1 SGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVAAAIGSDGAGSVRIPA
TH_4482|M.thermoresistible__bu SGPAFGHTRNPWSPRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSVRIPA
MMAR_3673|M.marinum_M SGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRVPA
MUL_3616|M.ulcerans_Agy99 SGPGFGHTRNPWARRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRVPA
MAV_2032|M.avium_104 SGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPA
MAB_1674c|M.abscessus_ATCC_199 SGPGFGHTRNPWSRGHTPGGSSGGSAAAVAAGLVAGAIGSDGAGSVRIPA
MSMEG_2986|M.smegmatis_MC2_155 DRSAHGASRNPWDPSRISGGSSGGSAVATAAGMVPLAVGTDTAGSVRVPA
Mb3409|M.bovis_AF2122/97 --PSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPT
Rv3375|M.tuberculosis_H37Rv --PSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIRFPT
MLBr_01702|M.leprae_Br4923 ENSAYGPTRNPWNVDRVPGGSGGGSAAALAAFQAPLAIGSDTGGSIRQPA
.. . *** .* *..*...* ** ::*:* .**:* *:
Mflv_2712|M.gilvum_PYR-GCK AWTHLVGIKPQRGRISTSPLPEAFNGITVNGVLARTVSDAAMVLDAAAGN
Mvan_3824|M.vanbaalenii_PYR-1 AWTHLVGIKPQRGRISTWPLTEAFNGITVHGVLARTVSDAALLLDAASGN
TH_4482|M.thermoresistible__bu AWTHLVGIKPQRGRISTWPLPEAFNGITVHGVLARTVTDAALVLDAASGN
MMAR_3673|M.marinum_M AWTHLVGIKPQRGRISTWPWPETFNGITVNGVLARTVADAALVLDAASGN
MUL_3616|M.ulcerans_Agy99 AWTHLVGIKPQRGRISTWPWPETFNGITVNGVLARTVADAALVLDAASGN
MAV_2032|M.avium_104 AWTHLVGIKPQRGRISTWPLPEAFNGITVNGVLARTVADAALVLDAASGN
MAB_1674c|M.abscessus_ATCC_199 AWTHLIGIKPQRGRISTWPFAEAFNGITVHGPLARTVADAALLLDAASGN
MSMEG_2986|M.smegmatis_MC2_155 ALCGVVGFKPAYGAIPAQGVWPLAASLDHVGVFARTATGAAAAYTAMAG-
Mb3409|M.bovis_AF2122/97 SMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGS
Rv3375|M.tuberculosis_H37Rv SMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGS
MLBr_01702|M.leprae_Br4923 ALTATVGVKPTYGTVSRYGLVACASSLDQGGPCARTVLDTALLHAVIAGH
: *.** * :. . * :*:. .:* . :*
Mflv_2712|M.gilvum_PYR-GCK AAGDLHTPAPVTVSDFVSRAPGP------LRVAVSTRFPFTGFRATLHPE
Mvan_3824|M.vanbaalenii_PYR-1 APGELHTPPPVRVSDYVSRAPGP------LRIAMSTKFPFTVFRAGLHPE
TH_4482|M.thermoresistible__bu APGDLHQPPPVRVSDYVGQAPGP------LRIAVSTKFPFTLFRAKLHPE
MMAR_3673|M.marinum_M VEGDLHKPAPLTASDYVGIAPGP------LKIAMSTRFPYTGFRAKLHPE
MUL_3616|M.ulcerans_Agy99 AEGDLHKPAPLTASDYVGIAPGP------LKIAMSTRFPYTGFRAKLHPE
MAV_2032|M.avium_104 VDGDRHKPPPITASDYVGKAPGP------LNIALSTRFPYTGFRPKLHPE
MAB_1674c|M.abscessus_ATCC_199 VPGDLHKPPHVRVLDAVHEDPGQ------LRVALSLKVPFSGFPAQLHPT
MSMEG_2986|M.smegmatis_MC2_155 ------------------RSPDP------VRAQRVAWLDPACLGPCDPAI
Mb3409|M.bovis_AF2122/97 DIHDPSCSAEPVPDYAADLALTR------IPRVGVDWSQTTSFDEDTTAM
Rv3375|M.tuberculosis_H37Rv DIHDPSCSAEPVPDYAADLALTR------IPRVGVDWSQTTSFDEDTTAM
MLBr_01702|M.leprae_Br4923 DARDSTSVEAAVPDIVGAAKAGESGDLHGVRVGVVRQLRGEGYQSGVLAS
: .
Mflv_2712|M.gilvum_PYR-GCK IRSALDGVADQLSQLGHTVLNADPDYTLGMSWNFLARSTSGIPEWMDR--
Mvan_3824|M.vanbaalenii_PYR-1 IRTALQNVADQLTQLGHTIVAADPDYHLRMSWNFLSRSTSGIPEWMDR--
TH_4482|M.thermoresistible__bu IRAAVQTVADQLEQLGHTVFPADPEYTVGMSWSFLSRSTAGLLDWSRR--
MMAR_3673|M.marinum_M ILAATRRVGEQLQLLGHTVERGNPDYGLRLSWDFLARSTAGLWEWAEC--
MUL_3616|M.ulcerans_Agy99 ILASTRRVGEQLQLLGHTVERGNPDYGLRLSWDFLARSTAGLWEWAEC--
MAV_2032|M.avium_104 ILAATRAVGKQLELLGHTVVPGNPDYGMRLSWDFLARSTAGLRDWEER--
MAB_1674c|M.abscessus_ATCC_199 IEAATRYVAHQLRSLGHMVSEGDPDYGVGLGLNFLPRATAGLLPWRDR--
MSMEG_2986|M.smegmatis_MC2_155 IAALSETLRAAQITVDGTVGLPFAAGEVFEALSVLQSSEAYTEYSAET--
Mb3409|M.bovis_AF2122/97 LADVVKTLDDIGWPVIDVKLPALAP--MVAAFGKMRAVETAIAHADTY--
Rv3375|M.tuberculosis_H37Rv LADVVKTLDDIGWPVIDVKLPALAP--MVAAFGKMRAVETAIAHADTY--
MLBr_01702|M.leprae_Br4923 FQAAVEQLIALGATVSEVDCPHFDYALAAYYLILPSEVSSNLARFDAMRY
: : : :
Mflv_2712|M.gilvum_PYR-GCK -----------------LGHRVTWDSRTRSNARMGRLLSQHTLRKARARE
Mvan_3824|M.vanbaalenii_PYR-1 -----------------LGRGVNWDSRTRSNARMGWLLSQNVLRKARAGE
TH_4482|M.thermoresistible__bu -----------------LGDNVLWDKRTVANMRLGRLLSESALRKARARE
MMAR_3673|M.marinum_M -----------------LGDGVTLDPRTVTNMRAGHALSQAILRNARHHE
MUL_3616|M.ulcerans_Agy99 -----------------LGNGVTLDPRTVTNMRAGHALSQAILRNARHHE
MAV_2032|M.avium_104 -----------------LGDGVVLDPRTVANLRLGHLLGQAILRSARRHE
MAB_1674c|M.abscessus_ATCC_199 -----------------LDAGAHWDPRTVTNMRTGRRLAGWALRRARAAE
MSMEG_2986|M.smegmatis_MC2_155 -----------------EQRASDIDPEVLGRLLRGRDTPAWQYVRACRQR
Mb3409|M.bovis_AF2122/97 -----------------PARADEYGPIMRAMIDAGHRLAAVEYQTLTERR
Rv3375|M.tuberculosis_H37Rv -----------------PARADEYGPIMRAMIDAGHRLAAVEYQTLTERR
MLBr_01702|M.leprae_Br4923 GLRVGDDGSRSAEEVIALTRAAGFGPEVKRRIMIGAYALSAGYYDSYYSQ
. * .
Mflv_2712|M.gilvum_PYR-GCK AAAQQRIGWIFNLADVVVAPTTAQPPPEAHAFDGLGGLATDRMMIRACPV
Mvan_3824|M.vanbaalenii_PYR-1 AASQQRIGWIFNLADVVLAPTTALPPPEVRAFDDLGGLATDRMMIKACPV
TH_4482|M.thermoresistible__bu AAAQRRMSWIFNIADVVLAPTTALPPPEVHAFDQLGGLATDRLMIKACPV
MMAR_3673|M.marinum_M AAAQRRVGSIFDIVDVVLAPTTAQPPPLARAFDDMGGLATDRAIIAACPL
MUL_3616|M.ulcerans_Agy99 AAAQRRVGSIFDIVDVVLAPTTAQPPPLARAFDDMGGLATDRAIIAACPL
MAV_2032|M.avium_104 AADQRRVGSIFDIVDVVLAPTTAQPPPLARAFDRLSGFGTDRAMIAACPL
MAB_1674c|M.abscessus_ATCC_199 PRLQARVGKIFGSFNVVLAPTTAQPPTGIYDFDDVGSLATDRGQVGACPM
MSMEG_2986|M.smegmatis_MC2_155 DRLRAAADTLLTRFDVLAMPAAPTVATEIDQRAHQIDGHAVEVRSALLSL
Mb3409|M.bovis_AF2122/97 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLG--QDPELTARLAMP
Rv3375|M.tuberculosis_H37Rv LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLG--QDPELTARLAMP
MLBr_01702|M.leprae_Br4923 AQKVRTLIARDLDKAYRSVDVLVSPATPTTAFSLGEKADDPLAMYLFDLC
..
Mflv_2712|M.gilvum_PYR-GCK TWPWNLLGWPSINVPAGFTSDG-LPIGVQLMGPADSEPLLISLAAALEAI
Mvan_3824|M.vanbaalenii_PYR-1 TWPWNLLGWPSINVPAGFTSDG-LPIGVQLMGPANSEPLLVSLAAALEAI
TH_4482|M.thermoresistible__bu TWPWNLLGWPSINVPAGFTSDG-LPIGVQLMGPANSEPLLVSLAAALEGI
MMAR_3673|M.marinum_M TWPWNLLGWPSINVPAGFTVDG-LPIGVQLMGPAESEGLLISLAAELEAV
MUL_3616|M.ulcerans_Agy99 TWPWNLLGWPSINVPAGFTVDG-LPIGVQLMGPAESEGLLISLAAELEAV
MAV_2032|M.avium_104 TFPWNVLGWPSINVPAGFTSDG-LPIGVQLMGPANSEGMLISLAAELEAV
MAB_1674c|M.abscessus_ATCC_199 TWPWNVLGWPSINVPAGFTADG-LPIGVQLMGPANSEPLLISLAAQLESI
MSMEG_2986|M.smegmatis_MC2_155 TCPWNLTGHPALSVPAGVVSG--LPVGLQLISAPGNEGMLFDLAARIEHR
Mb3409|M.bovis_AF2122/97 TAPFNVSGNPAICLPAGTTARG-TPLGVQFIGREFDEHLLVRAGHAFQQV
Rv3375|M.tuberculosis_H37Rv TAPFNVSGNPAICLPAGTTARG-TPLGVQFIGREFDEHLLVRAGHAFQQV
MLBr_01702|M.leprae_Br4923 TLPLNLAGHCGMSVPSGLSSDDDLPVGLQIMAPALADDRLYRVGAAYEAA
* * *: * .: :*:* *:*:*::. : * . :
Mflv_2712|M.gilvum_PYR-GCK NGWATLQPENWWAPQPPTEPDL----TAPDSVTGQVA
Mvan_3824|M.vanbaalenii_PYR-1 NGWAVHQPEDWWTPQPPPEPDLGSNVAAPASVEGQVA
TH_4482|M.thermoresistible__bu NGWAARQPRPWWLT-PPAAPXX----XASAAAS----
MMAR_3673|M.marinum_M SGWATKQPKVWWNTGTSTPPIH----SVRPPRR----
MUL_3616|M.ulcerans_Agy99 SGWATKQPKVWWNTGTSTPPIH----SVRPPRR----
MAV_2032|M.avium_104 SGWASKQPQPWWDQSADTPPAP----GQPAPTG----
MAB_1674c|M.abscessus_ATCC_199 NNWASEIPDPWW-------------------------
MSMEG_2986|M.smegmatis_MC2_155 -------------------------------------
Mb3409|M.bovis_AF2122/97 TGYHRRRPPV---------------------------
Rv3375|M.tuberculosis_H37Rv TGYHRRRPPV---------------------------
MLBr_01702|M.leprae_Br4923 RGPLPSAI-----------------------------