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RTENQVLERIDQFRQRRPRLLDDVVTLAHGAGGKSSAALVDAVFLEAFRNPELEQLGDAALVRTPSGDTL AFTTDSYVVAPHRFPGGSIGHVAVHGTINDLAVSGARPQWLSAGFVIEEGFPIAELREIVADMSEAAARA GVHIVTGDTKVVGKGAADGLYICTAGVGVVPAGRRLSRADVRPGDQVVLSGTIGEHGMAVMLARGDLAID ADIESDTAPVHDIVEILLAAAPSTRWMRDATRGGVGTVSNELAKDAPYAVILDEQRLPVAPQVLGACDML GIDPLYVANEGKFLAVVPHDEAAAAVAALRAHPQGANAAIVGEITSEPAGIVALRTSFGGSRIVDMLVGD PLPRIC*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_2705 | hypE | - | 100% (357) | hydrogenase expression/formation protein HypE |
| M. smegmatis MC2 155 | MSMEG_2276 | hypE | 6e-81 | 47.31% (334) | hydrogenase expression/formation protein HypE |
| M. smegmatis MC2 155 | MSMEG_5798 | purM | 9e-08 | 30.67% (150) | phosphoribosylaminoimidazole synthetase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0832 | purM | 2e-07 | 31.15% (122) | phosphoribosylaminoimidazole synthetase |
| M. gilvum PYR-GCK | Mflv_3982 | - | 2e-77 | 43.98% (332) | hydrogenase expression/formation protein HypE |
| M. tuberculosis H37Rv | Rv0809 | purM | 2e-07 | 31.15% (122) | phosphoribosylaminoimidazole synthetase |
| M. leprae Br4923 | MLBr_02205 | purM | 6e-08 | 30.43% (184) | phosphoribosylaminoimidazole synthetase |
| M. abscessus ATCC 19977 | MAB_0728 | - | 6e-10 | 34.75% (118) | phosphoribosylaminoimidazole synthetase |
| M. marinum M | MMAR_1859 | hypE | 1e-146 | 71.47% (354) | hydrogenase maturation factor, HypE |
| M. avium 104 | MAV_0748 | purM | 3e-08 | 33.05% (118) | phosphoribosylaminoimidazole synthetase |
| M. thermoresistible (build 8) | TH_1944 | hypE | 1e-159 | 79.10% (354) | hydrogenase expression/formation protein HypE |
| M. ulcerans Agy99 | MUL_0418 | purM | 6e-09 | 33.06% (121) | phosphoribosylaminoimidazole synthetase |
| M. vanbaalenii PYR-1 | Mvan_2404 | - | 4e-86 | 47.89% (332) | hydrogenase expression/formation protein HypE |
CLUSTAL 2.0.9 multiple sequence alignment
MAV_0748|M.avium_104 --------------------MTDPGKSP---GRHSGSQGITYASAGVDIE
MUL_0418|M.ulcerans_Agy99 MRGARVADTGGERRPVAFIAMTDPEKSPESSGRGPASQGVTYAAAGVDIE
MLBr_02205|M.leprae_Br4923 --------------------MMDPGKSP---GGQLGNHGITYASTGVDIE
Mb0832|M.bovis_AF2122/97 --------------------MTDLAKGP---GKDPGSRGITYASAGVDIE
Rv0809|M.tuberculosis_H37Rv --------------------MTDLAKGP---GKDPGSRGITYASAGVDIE
MAB_0728|M.abscessus_ATCC_1997 --------------------MPDMSERA----DKPSN---SYAAAGVDIE
MSMEG_2705|M.smegmatis_MC2_155 -----MRT-------------ENQVLERIDQFRQRRPRLLDDVVTLAHGA
TH_1944|M.thermoresistible__bu -----MSTDA---------AREEQVRNRIDRFRRRRPRLLDEVVTLAHGA
MMAR_1859|M.marinum_M -----MSKSAGEYLSSGPRFAEGDVIGRIESFRRRRPRLLDEHVTLAHGA
Mflv_3982|M.gilvum_PYR-GCK -MPDTEPEASIETEPEASIETEPEASIDMESWVCPLPLRDAPAIVTGHGG
Mvan_2404|M.vanbaalenii_PYR-1 -MP--EPDQRIR------------AAIDVESWVCPAPLRDAPNIVMGHGG
. .
MAV_0748|M.avium_104 AGDRAVELFKPLATKATRPEVRGGLGGFAGLFALRGEYREPLLAASTDGV
MUL_0418|M.ulcerans_Agy99 AGDRAVELFKPLATRATRPEVRGGLGGFAGLFALRNDYREPVLAASTDGV
MLBr_02205|M.leprae_Br4923 AGDRSVELFKPMAVKAIRTEVLGGLGGFAGLFALRSGYREPVLAASTDGV
Mb0832|M.bovis_AF2122/97 AGDRAIDLFKPLASKATRPEVRGGLGGFAGLFTLRGDYREPVLAASSDGV
Rv0809|M.tuberculosis_H37Rv AGDRAIDLFKPLASKATRPEVRGGLGGFAGLFTLRGDYREPVLAASSDGV
MAB_0728|M.abscessus_ATCC_1997 AGDRAVELFKKSVAKTHRPEVLGGIGGFAGLFALKGGYREPVLAASTDGV
MSMEG_2705|M.smegmatis_MC2_155 GGKSSAALVDAVFLEAFRNPELEQLG-DAALVRTPSGDTLAFTTDS----
TH_1944|M.thermoresistible__bu GGKSSAALTDAVFLDAFRNAELEQLD-DAAVLATASGQRLAFSTDS----
MMAR_1859|M.marinum_M GGKASAALIDAVFMEAFRNPLLESLG-DGAILTLPSGERVAMSTDS----
Mflv_3982|M.gilvum_PYR-GCK GGAMSGELVEHLFLPAFGSAAEAGLA-DSAVVEIG-GARLAFSTDS----
Mvan_2404|M.vanbaalenii_PYR-1 GGAMSGELIEHLFLPAFGPAADAAMG-DAAVVEIG-GTRLAFSTDS----
.* : * . : : ..:. .. : *
MAV_0748|M.avium_104 GTKLAVAQAMDKHDTVGLDLVAMVVDDLVVCGAEPLFLQDYIAVGR-TVP
MUL_0418|M.ulcerans_Agy99 GTKLAVAQAMDKHDTVGLDLVAMVVDDLVVCGAEPLFLQDYIAVGR-TVP
MLBr_02205|M.leprae_Br4923 GTKLAVAQAMDKHDTVGLDLVAMVVDDLVVCGAEPLFLQDYIAVGR-TVP
Mb0832|M.bovis_AF2122/97 GTKLAIAQAMDKHDTVGLDLVAMVVDDLVVCGAEPLFLLDYIAVGR-IVP
Rv0809|M.tuberculosis_H37Rv GTKLAIAQAMDKHDTVGLDLVAMVVDDLVVCGAEPLFLLDYIAVGR-IVP
MAB_0728|M.abscessus_ATCC_1997 GTKIAVAQAMDKRDTVGLDLVAMVVDDLVVCGAEPLFLQDYIAVGK-VVP
MSMEG_2705|M.smegmatis_MC2_155 ---YVVAPHRFPGGSIGHVAVHGTINDLAVSGARPQWLSAGFVIEEGFPI
TH_1944|M.thermoresistible__bu ---YVVQPLRFPGGSIGELAVHGTVNDLAVSGAIPQWLAAGFVIEEGFPI
MMAR_1859|M.marinum_M ---FVVQPRRFPGGSIGELAVHGTCNDLAVAGAVPSWISAAFVLEEGFPI
Mflv_3982|M.gilvum_PYR-GCK ---FVVKPMVFPGGTIGDLAVNGTVNDLAMAGAVPAVLSTAFILEEGTAL
Mvan_2404|M.vanbaalenii_PYR-1 ---FVVKPMVFPGGSIGDLAVNGTVNDLAMAGAVPQVLSTAFILEEGTPL
.: .::* * . :**.:.** * : : : .
MAV_0748|M.avium_104 ERLSAIVSGIAEGCVRAGCALLGGETAEHPGLMEPDHYDISATGVGVVEA
MUL_0418|M.ulcerans_Agy99 ERVSAIVSGIAEGCVRAGCALLGGETAEHPGLMAPDHYDISATGVGVVEA
MLBr_02205|M.leprae_Br4923 ERLSAIVDGIAEGCMLAGCALLGGETAEHPGLMEVDHYDIAATGVGVVEA
Mb0832|M.bovis_AF2122/97 ERLSAIVAGIADGCMRAGCALLGGETAEHPGLIEPDHYDISATGVGVVEA
Rv0809|M.tuberculosis_H37Rv ERLSAIVAGIADGCMRAGCALLGGETAEHPGLIEPDHYDISATGVGVVEA
MAB_0728|M.abscessus_ATCC_1997 ERVSELVAGIAEGCAIAGCALLGGETAEHPGLMAPDDFDLSATGVGIVEA
MSMEG_2705|M.smegmatis_MC2_155 AELREIVADMSEAAARAGVHIVTGDTKVVG-KGAADGLYICTAGVGVVPA
TH_1944|M.thermoresistible__bu ADLRAIVADMAAAAARAGVLIVTGDTKVVG-RGAADGVYITTTGVGVIPE
MMAR_1859|M.marinum_M EELKQIVADMADAAAKAGVQIVTGDTKVVP-KGAADGLFITTTGTGIIPT
Mflv_3982|M.gilvum_PYR-GCK DHLARVAQAVGTAALAAGVKLVTGDTKVVD-SGHGDGVYINTTGIGLVDP
Mvan_2404|M.vanbaalenii_PYR-1 DELARVAQAVGTAAMAAGVRLVTGDTKVVD-SGHGDGVYINTAGIGLVDD
: :. :. .. ** :: *:* * : ::* *::
MAV_0748|M.avium_104 DDVLGPDRVKPGDVIIAMGSSGLHSNGYSLARTVLLEIDRMNLEGYVEEF
MUL_0418|M.ulcerans_Agy99 DNVLGPERVKPGDVIIAMGSSGLHSNGYSLARNVLLEIDRMNLAGYVEEF
MLBr_02205|M.leprae_Br4923 DDTLGPDRVKPGDVIIALRSSGLHSNGYSLARSVLLEIDRMNLAGYVEEF
Mb0832|M.bovis_AF2122/97 DNVLGPDRVKPGDVIIAMGSSGLHSNGYSLVRKVLLEIDRMNLAGHVEEF
Rv0809|M.tuberculosis_H37Rv DNVLGPDRVKPGDVIIAMGSSGLHSNGYSLVRKVLLEIDRMNLAGHVEEF
MAB_0728|M.abscessus_ATCC_1997 DRVLGPDRVRPGDVVIAMGSSGLHSNGYSLARHVLLEIDRMDLFGQVEEF
MSMEG_2705|M.smegmatis_MC2_155 GRRLSRADVRPGDQVVLSGTIGEHGMAVMLAR-GDLAIDADIESDTAPVH
TH_1944|M.thermoresistible__bu GRHLARERIRVGDKVVLSGSIGAHGMAVMLAR-GDLAIDADIVSDTAPVH
MMAR_1859|M.marinum_M GRALSADSVRPGDKVLLSGSMGDHGMAVMLAR-GDLAIEADIHSDTASVS
Mflv_3982|M.gilvum_PYR-GCK RVDIRPQRAAPGDVVIVSGDLGVHGIAVLSCR-EGLEFATSVASDAAPLH
Mvan_2404|M.vanbaalenii_PYR-1 RTDIRPQRAAPGDAVIVSGDIGVHGVAVLSCR-EGLEFGTAVASDTAPLH
: ** :: * *. . * * : . .
MAV_0748|M.avium_104 GRTLGEELLEPTRIYAKDCLALAAETHVRTFCHVTGGGLAGNLARVIPHG
MUL_0418|M.ulcerans_Agy99 GRTLGEELLEPTRIYAKDCLALTAETHVRTFCHVTGGGLAGNLERVIPHG
MLBr_02205|M.leprae_Br4923 GRTLGEELLEPTRIYAKDCLALAAETHVRTFCHVTGGGLAGNLQRVIPHG
Mb0832|M.bovis_AF2122/97 GRTLGEELLEPTRIYAKDCLALAAETRVRTFCHVTGGGLAGNLQRVIPHG
Rv0809|M.tuberculosis_H37Rv GRTLGEELLEPTRIYAKDCLALAAETRVRTFCHVTGGGLAGNLQRVIPHG
MAB_0728|M.abscessus_ATCC_1997 GRTLGEELLEPTRIYAKDCLALAAETEVRTFCHVTGGGLAGNLARVIPDG
MSMEG_2705|M.smegmatis_MC2_155 DIVEILLAAAPSTRWMRDATRGGVGTVSNELAKDAPYAVILDEQRLP---
TH_1944|M.thermoresistible__bu ELVEALLAAAPSTRWMRDATRGGVATVCNELVSGAPFAVVLAEDRVP---
MMAR_1859|M.marinum_M PLVELLMAAAPGTRWLRDATRGGVGTVCNELAQACGFGVLLDEQRLP---
Mflv_3982|M.gilvum_PYR-GCK GLVAAMVETGADIHTLRDPTRGGLAATLNEIARAAGVGVSIADTALP---
Mvan_2404|M.vanbaalenii_PYR-1 GLVAAMVATGADVHALRDPTRGGMAATLNEIAKAAKVGVTIDERALP---
. . :* : . : .: :
MAV_0748|M.avium_104 LVAELDRGTWTPAPVFAMIAQRGRVTREEMEKTFNMGVGMIAVVAPEDTD
MUL_0418|M.ulcerans_Agy99 LVAEIDRGTWTPAPVFAMIAQRGRVVREEMEKTFNMGVGMIAVVAPEDTD
MLBr_02205|M.leprae_Br4923 LVAEVDRGTWTPAPVFAMIAQRGRVAREEMEKTFNMGVGMIAVVAPEDTD
Mb0832|M.bovis_AF2122/97 LIAEVDRGTWTPAPVFTMIAQRGRVRRTEMEKTFNMGVGMIAVVAPEDTT
Rv0809|M.tuberculosis_H37Rv LIAEVDRGTWTPAPVFTMIAQRGRVRRTEMEKTFNMGVGMIAVVAPEDTT
MAB_0728|M.abscessus_ATCC_1997 LVAELDRGSWTPGPIFAMIAQRGRIERAEMEKTFNMGVGMVAVVAPEDVD
MSMEG_2705|M.smegmatis_MC2_155 VAPQVLGACDMLGIDPLYVANEGKFLAVVPHDEAAAAVAALRAHPQGANA
TH_1944|M.thermoresistible__bu VQPQVLGACDLLGIDPLYVANEGAFIAVVPADEAAAGVAALHDHPSGRHA
MMAR_1859|M.marinum_M VAPMVNGACELLGIDPLYVANEGKFIAVVAPEEAEAGLAALQSHPLGADA
Mflv_3982|M.gilvum_PYR-GCK IPQEVRDACSMLGLDPLYVANEGKLVAFVPAADADEVLAAMHAHPLGAQS
Mvan_2404|M.vanbaalenii_PYR-1 IPSEVRDACSMLGLDPLYVANEGKLVAFVPAADAGKVLAAMRAHPLGAHA
: : . . :*:.* . :. : .
MAV_0748|M.avium_104 RALAILTARHLDCWVLGTVGKGGKAG--PRAKLVGQHPRF-
MUL_0418|M.ulcerans_Agy99 RALAILTARHLDCWVLGTVCKGGKEG--PRATLVGQHPRF-
MLBr_02205|M.leprae_Br4923 RALAILTARHLDCWVLGTVCKGSKVG--PRAKLVGQHPRF-
Mb0832|M.bovis_AF2122/97 RALAVLTARHLDCWVLGTVCKGGKQG--PRAKLVGQHPRF-
Rv0809|M.tuberculosis_H37Rv RALAVLTARHLDCWVLGTVCKGGKQG--PRAKLVGQHPRF-
MAB_0728|M.abscessus_ATCC_1997 RALAVLTARHIDCWTLGTIKKSGKDSGSESALLVGQHPRF-
MSMEG_2705|M.smegmatis_MC2_155 AIVGEITSEPAGIVALRTSFGGSRIV---DMLVGDPLPRIC
TH_1944|M.thermoresistible__bu AVVGEVVGEPTGIVALRTSFGGSRIV---DMLVGDPLPRIC
MMAR_1859|M.marinum_M AEVGEIIAEPAETVILRTGFGGTRIV---DMLVGDPLPRIC
Mflv_3982|M.gilvum_PYR-GCK AVIGTCVPDHPGMVVAKTALGGNRVV---DLPIGEQLPRIC
Mvan_2404|M.vanbaalenii_PYR-1 VIIGACVADHPGMVVAKTALGGTRVV---DLPIGEQLPRIC
:. * . : : **: