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MSQRIVPTVNSSTVFRNGVIWSPDGNTTTDALLISGGVVQAVGDAAKAGSADVEVDLEGGFLMPSFGDGH AHPLYGGLESAGPAVRKGKSVDEIVAAVKEYADAHPEQEWIVGASYDGSLASDGLFDARWLDAVVPDRPV VLRAWDYHTFWVNSAALQRAGIAADTPDPVMGEIPRRADGSPLGTLREWGATDLVSAVMPARDESVRVGA LSTAADYYLARGVTWVQDAWVEPADVDTYVAAAQQGALRMRFNLAFYADPRHFDEQVTQYAAARDRVRAA GSDLLTAQTVKFFADGVVENETGALLAPYCSGLHSHGMQVWEGDSLAQAARRVDDLGLQIHIHAIGDAAV RQALDAIEYVVRQNGPRDRRPVIAHAQLVDDADLGRFAELGVIPNMQPLWAQMDALMTVLTIPRLGVERA QKQYRIRSLENSGAALAFGSDWPVSSGAPLDGIAVAVSRRTADGEPEGGWTPHEILPPEAALASYTGAVA YQAFAEKTWGRIVPGASADLVWLDRDPRTVPPLELPAVGVRATYLQGRPAHTAAD
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_0445 | - | - | 100% (545) | amidohydrolase family protein |
| M. smegmatis MC2 155 | MSMEG_4289 | - | 3e-67 | 35.23% (562) | amidohydrolase |
| M. smegmatis MC2 155 | MSMEG_1102 | - | 6e-33 | 26.46% (548) | twin-arginine translocation pathway signal |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb0567 | - | 2e-38 | 27.72% (552) | hypothetical protein Mb0567 |
| M. gilvum PYR-GCK | Mflv_2930 | - | 2e-59 | 31.12% (556) | amidohydrolase 3 |
| M. tuberculosis H37Rv | Rv0552 | - | 2e-38 | 27.72% (552) | hypothetical protein Rv0552 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_2546 | - | 4e-64 | 32.54% (547) | amidohydrolase |
| M. marinum M | MMAR_1131 | - | 3e-36 | 27.63% (561) | metal-dependent hydrolase |
| M. avium 104 | MAV_4591 | - | 2e-39 | 28.73% (550) | amidohydrolase family protein |
| M. thermoresistible (build 8) | TH_0643 | - | 1e-06 | 28.16% (206) | amidohydrolase 3 |
| M. ulcerans Agy99 | MUL_0651 | - | 1e-35 | 27.51% (567) | hypothetical protein MUL_0651 |
| M. vanbaalenii PYR-1 | Mvan_1184 | - | 0.0 | 76.81% (526) | amidohydrolase 3 |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_0445|M.smegmatis_MC2_155 ------------------------------------------------MS
Mvan_1184|M.vanbaalenii_PYR-1 ------------------------------------------------MT
Mflv_2930|M.gilvum_PYR-GCK MCTACEWAPHFAAYGRRNPVTRRTALRAAAVTALAVTAAACATGPDSDTS
MAB_2546|M.abscessus_ATCC_1997 MCTACEWAPHFAALSAKS--TRRTALRAAVALTLAAGAAACSAGTVPAPT
Mb0567|M.bovis_AF2122/97 --------------------------------------------------
Rv0552|M.tuberculosis_H37Rv --------------------------------------------------
MUL_0651|M.ulcerans_Agy99 --------------------------------------------------
MAV_4591|M.avium_104 --------------------------------------------------
MMAR_1131|M.marinum_M --------------------------------------------------
TH_0643|M.thermoresistible__bu --------------------------------------------------
MSMEG_0445|M.smegmatis_MC2_155 --QRIVPTVNSSTVFRNGVIWSPDGNT-TTDALLISGGVVQAVGDAAKA-
Mvan_1184|M.vanbaalenii_PYR-1 NDTEIAVLTAPPTLFTGGTIWTGRGD---TDAVLVDGGVVVALGDEARR-
Mflv_2930|M.gilvum_PYR-GCK VTSAASGAEAPDFVFHNGRVYTVTESAPWAEAVVVTGNSITFVGDNAAAR
MAB_2546|M.abscessus_ATCC_1997 TGSAGPQNTHADFVFRNGRVYTVAGPTEWAQAVAVQGNAITHVGDEAGAM
Mb0567|M.bovis_AF2122/97 --------MADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVA
Rv0552|M.tuberculosis_H37Rv --------MADADLVMTGTVLTVDDARPTAEAIAVADGRVIAVGDRSEVA
MUL_0651|M.ulcerans_Agy99 --------MADADVVITGTVLTVDDTRPTAEAIAVTDGRVVAVGDRSEVA
MAV_4591|M.avium_104 ------MHMGQADLVLTGTVLTVDEARPTAEALAVADGRIVAVGTRAEIA
MMAR_1131|M.marinum_M ------MTGDADAIYTNGDIVTVDDEQPIAEAVAVKDGRIVAVGAHDDVV
TH_0643|M.thermoresistible__bu --------------------------------------------------
MSMEG_0445|M.smegmatis_MC2_155 ----GSADVEVDLEGGFLMPSFGDGHAHPLYGGLESAG-----PAVRKGK
Mvan_1184|M.vanbaalenii_PYR-1 ----RGGAHHVDLDGGFLMPSFADGHAHPLYGGLEAVG-----PPVRRCG
Mflv_2930|M.gilvum_PYR-GCK -ALAGPDTEVIDLAGKLLMPGFVEGHIHPFLGSFLSTG-----VDLQVP-
MAB_2546|M.abscessus_ATCC_1997 -ALVGPNTRVVDLAGRLLMPGFVEGHIHPFFGAFLSGG-----VNLQVS-
Mb0567|M.bovis_AF2122/97 -GLVGANTRVIDLGAGCVMPGFVEAHGHPLLEAVVLSDR-FVDIRPVTMR
Rv0552|M.tuberculosis_H37Rv -GLVGANTRVIDLGAGCVMPGFVEAHGHPLLEAVVLSDR-FVDIRPVTMR
MUL_0651|M.ulcerans_Agy99 -GLIGEGTRTIDLGSGCLMPGFVEAHGHPLMEAIVLSGR-IVDIRPVTVR
MAV_4591|M.avium_104 -AHVGPHTQTVDIGDGCVMPGFVEAHGHPLMEAVALSDR-IVDIRPVTLA
MMAR_1131|M.marinum_M REHLGPHTRRVDLAGNTLLPGFIDPHSHYINALTVANQVNVFAPPAGPAA
TH_0643|M.thermoresistible__bu --------------------------------------------------
MSMEG_0445|M.smegmatis_MC2_155 SVDEIVAAVKEYADAH--PEQEWIVGASYDGSLASDG-LFDARWLDAVVP
Mvan_1184|M.vanbaalenii_PYR-1 SVDEIVRAVQRYAADH--PDEDWIVGASYDSSLAPEG-LFDARWLDAAVP
Mflv_2930|M.gilvum_PYR-GCK TRQDALNAIAAYATDK--ADG-PVRGFGWRVDMFGPQ-GPTRTELDSVLP
MAB_2546|M.abscessus_ATCC_1997 TKEAALAAIAKYAEEN--PVG-PVRGFGWRVDMFGPE-GPTRQELDSVLP
Mb0567|M.bovis_AF2122/97 DADDVVAAIRGEVARR--GPAGAYLVGWDPLLQSGLG-EPTLTWLDSLAP
Rv0552|M.tuberculosis_H37Rv DADDVVAAIRGEVARR--GPAGAYLVGWDPLLQSGLG-EPTLTWLDSLAP
MUL_0651|M.ulcerans_Agy99 DSERVVAEICREAGER--GSAGAYLIGWDPLLQPGLP-EPTLAWLDDIAP
MAV_4591|M.avium_104 NADDVVAAVKSEVGKR--GEAGAYLNGWDPLLQQGLP-QPTLAWLDDIAP
MMAR_1131|M.marinum_M DVEAIVAELKKFRDARDIADGEIIMAYGYDETVMPDGRTLHREDLDADFP
TH_0643|M.thermoresistible__bu --------------------------------------------------
MSMEG_0445|M.smegmatis_MC2_155 DRPVVLRAWDYHTFWVNSAALQRAGIAADTPDPVMG--EIPRRADGSPLG
Mvan_1184|M.vanbaalenii_PYR-1 DRPVVLRAWDYHTVWCNSAALQRAGITAETPEPVLG--EIPRRADGSPLG
Mflv_2930|M.gilvum_PYR-GCK DRPGFFFAIDGHSMWANSKALEMAGISRDSEDPIPGFSYYVRDENGEPTG
MAB_2546|M.abscessus_ATCC_1997 DRPGFFFAVDAHSMWANSKSLEVAGINRETPDPVPGFSYYAKDSHGEPTG
Mb0567|M.bovis_AF2122/97 NGPLVIIHNSGHKAYFNSHAAWLNGLTRDTADPKGA--KYGRDGNGELDG
Rv0552|M.tuberculosis_H37Rv NGPLVIIHNSGHKAYFNSHAAWLNGLTRDTADPKGA--KYGRDGNGELDG
MUL_0651|M.ulcerans_Agy99 NGPLVIIHNSGHKAYFNTHAAWLNGLTRDTPDPKGA--KYGRDANGELDG
MAV_4591|M.avium_104 DGPLVIIHNSGHKAFFNSRAAQRHGLNRDTPDPKGA--RYGRDADGELDG
MMAR_1131|M.marinum_M NNPVLVGHVSLHGAVLNSAAMQKFGISADTETPPGG-VIVRKEGSTEPDG
TH_0643|M.thermoresistible__bu -VPVRVQHRSGVLWTLNSAGLARIGMPDHPDGRLRS--------------
* . . *: . *: .. .
MSMEG_0445|M.smegmatis_MC2_155 TLREWGATDLVSAVMPARDESVRVGALSTAADYYLARGVTWVQDAWV---
Mvan_1184|M.vanbaalenii_PYR-1 TLREWGATDLVMNAMPPRDEAVRVGALGTAADYYLSRGVTWVQDAWV---
Mflv_2930|M.gilvum_PYR-GCK YVLEVDAVLGLVNAIEPISTDSMEQLLRAWLPKASEAGITSVFDAGVPPI
MAB_2546|M.abscessus_ATCC_1997 FILEISAILDLVNAIEPVTAQSLQRALEDWLPNAAAAGITSVFDAGVPAV
Mb0567|M.bovis_AF2122/97 TAEEIGAILPLLAGVAD--PSNFGAMLRAECARLNRAGLTTCSEMAFDP-
Rv0552|M.tuberculosis_H37Rv TAEEIGAILPLLAGVAD--PSNFGAMLRAECARLNRAGLTTCSEMAFDP-
MUL_0651|M.ulcerans_Agy99 TAEETGAVLPLLAGVTD--PSNYPAMLRAECARLNRAGLTTCLEMAFDP-
MAV_4591|M.avium_104 TAEETGAVFPLLDGAID--TAGYPRMLHAECARLNRAGLTTCSEMAFDP-
MMAR_1131|M.marinum_M LIMET-AFLPIFASLPKPTPEQEVQWSIAGQLLYAAVGITTAHEGLTHAA
TH_0643|M.thermoresistible__bu ------ADPDWSNSLQR--RETRIADLSAELTRYGVTGVTDATPGLDVG-
* *:*
MSMEG_0445|M.smegmatis_MC2_155 ---EPADVDTYVAAAQQGALRMRFNLAFYADPRHFDEQVTQYAAARDRVR
Mvan_1184|M.vanbaalenii_PYR-1 ---EPADVATYVAAAGQGALRMRFNLALYADPRHFDAQVTQFAEQRALVE
Mflv_2930|M.gilvum_PYR-GCK GGDQGALIELYTNVESRGELPFRVVASYSVKSPPVDDAVAELTGIRDRIS
MAB_2546|M.abscessus_ATCC_1997 AS-QAALIGLYGDIERRGRLPFRVVASYTVKAPPFGDVVAKFAETRDQVS
Mb0567|M.bovis_AF2122/97 -----------------GYRPMVEAVRAELTVRLCTYEISNARMCTDATP
Rv0552|M.tuberculosis_H37Rv -----------------GYRPMVEAVRAELTVRLCTYEISNARMCTDATP
MUL_0651|M.ulcerans_Agy99 -----------------GFRPLVEQLRDELTVRLRTYEISNAQLSTEASP
MAV_4591|M.avium_104 -----------------RFRPMVEQLRGQLTVRLRSYEISNPQLRTDATP
MMAR_1131|M.marinum_M D-----------VALLRRAAAGGADLIDVIAYPFILELDEVLPENPADTF
TH_0643|M.thermoresistible__bu --------------------------------------------------
MSMEG_0445|M.smegmatis_MC2_155 AAGSDLLTAQTVKFFADGVVENETGALLAPYCSGLHSHG---------MQ
Mvan_1184|M.vanbaalenii_PYR-1 AARSPLLTAQTVKFFADGVVENETGALLAPYCTGLHDHAG--------QH
Mflv_2930|M.gilvum_PYR-GCK ---SDLVQVNVVKIIGDGTQEGYTAWLLEPYADKPDSIG---------GS
MAB_2546|M.abscessus_ATCC_1997 ---TDLVRVGAVKIMGDGTQGGYTAWLIDPYADKPDSIG---------TS
Mb0567|M.bovis_AF2122/97 GQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTPATRAIGVPPGSRGCA
Rv0552|M.tuberculosis_H37Rv GQGDDMLRQVGIKIWVDGSPWVGNIDLTFPYLDTPATRAIGVPPGSRGCA
MUL_0651|M.ulcerans_Agy99 GDGDDMLRQVGIKIWVDGSPWIGNIDLSFPYLDTAATRTIGVTPGSCGCA
MAV_4591|M.avium_104 GEGDDMLRQVGIKIWVDGSPWIGNIALSFPYLDTEATRTIGVTPGSCGCA
MMAR_1131|M.marinum_M GTYHNRLKLGGVKITLDGSPQGRTAFFTAPYLADGPGGEKNWSG----EL
TH_0643|M.thermoresistible__bu ----DVVRLTGMNRRGNLSQRVNCLAPGKRILHD---------------D
: :: :
MSMEG_0445|M.smegmatis_MC2_155 VWEGDSLAQAARRVDDLGLQIHIHAIGDAAVRQALDAIEYVVRQNGPRDR
Mvan_1184|M.vanbaalenii_PYR-1 NWEGESLAEAARRVDELGLQIHIHAIGDAAVRQALDAIEYVAAQNGPRDR
Mflv_2930|M.gilvum_PYR-GCK PFTDDQWRQLVREVDAAGFDVHIHACGERTARTALDSIEAAAKVNPARER
MAB_2546|M.abscessus_ATCC_1997 PFTEQEWGELVGRIDSAGIDMHVHACGERTARVALDAFEAAIANNPARDR
Mb0567|M.bovis_AF2122/97 NYTREQLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRNPRDDH
Rv0552|M.tuberculosis_H37Rv NYTREQLAEIVGAYFPRGWQIACHVHGDGGVDTILDVYEEALRRNPRDDH
MUL_0651|M.ulcerans_Agy99 NYTREQLAETVGAYFPLGWPIACHVQGDAGVDTTLDVYEEALRSNPRPDH
MAV_4591|M.avium_104 NYTTEQLREIVGAYFPLGWQLACHVQGDAGVDTILDVYEEALKRHPRDDH
MMAR_1131|M.marinum_M PFSQETVNGWFKRVYDLGLPLNIHANGDGAIDVLLAAHEYAAADDPTKDR
TH_0643|M.thermoresistible__bu RLDLTELTEWIATRHAAGGAVAIHCVTAAQLVVTLTALRAVGTHPQDR--
* : * * . .
MSMEG_0445|M.smegmatis_MC2_155 RPVIAHAQLVDDADLGRFAELGVIPNMQPLWAQMDALMTVLTIPRLGVER
Mvan_1184|M.vanbaalenii_PYR-1 RAVIAHCQLVDRDDLDRFAALSVIPNMQPLWAQLDALMTVLTIPRLGADR
Mflv_2930|M.gilvum_PYR-GCK RHTVAHLVYLEEPDSRRFGDLGVIAEFSANWFSADPDTVINLAARYGAPR
MAB_2546|M.abscessus_ATCC_1997 RHTVAHLIYVDDADSARFAKLGVVAQFSANWFSADPDTVVNMAARYGAPR
Mb0567|M.bovis_AF2122/97 RLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYWGDVIVD--DLFGAQR
Rv0552|M.tuberculosis_H37Rv RLRLEHVGAIRPDQLRRAAELGVTCSIFVDQIHYWGDVIVD--DLFGAQR
MUL_0651|M.ulcerans_Agy99 RLRLEHVGAIRPEQLRRAADLGVTCSIFVDQIHYWGDAIVD--GLFGPER
MAV_4591|M.avium_104 RLRLEHVGAIRPDQLQRAADLGVTCSIFVDQIHYWGDVLVD--GLFGEER
MMAR_1131|M.marinum_M HTTVIHSQFVRRDQLAKYVEYNLIPSLFTEHAFYFGDTHVR---LRGKEQ
TH_0643|M.thermoresistible__bu ---IEHAAVVPDDSLEEIAALGLTVVTQPNFIAERGDQYRT---DVPADE
: * : . . .: .
MSMEG_0445|M.smegmatis_MC2_155 AQKQYRIRSLENSGAALAFGSDWPVS----SGAPLDGIAVAVSRRTADGE
Mvan_1184|M.vanbaalenii_PYR-1 ADSQYPIRTLDASGAPLAFGSDWPVS----SGAPLDGVAVAASRTTSDGD
Mflv_2930|M.gilvum_PYR-GCK KDNLYRIQDVLSSGGRVSLGTDWPAAGYFSTYKPLDSIQIGVTRQLIGDP
MAB_2546|M.abscessus_ATCC_1997 KDNFYRIQDILRTGAHVALGTDWPAAGYFSTYKPLESIQIGVTRQLIGDP
Mb0567|M.bovis_AF2122/97 GSRWMPAGSAVAAGMRISLHNDPPVT----PEEPLRNISVAATRVAPSG-
Rv0552|M.tuberculosis_H37Rv GSRWMPAGSAVAAGMRISLHNDPPVT----PEEPLRNISVAATRVAPSG-
MUL_0651|M.ulcerans_Agy99 GSRWMPAGSAVATGMRISLHNDPPVT----PEEPLRNISVAATRVAPSD-
MAV_4591|M.avium_104 GSRWMPAGSAVATGMRISLHNDPPVT----PEEPLRNISVAATRKAPSG-
MMAR_1131|M.marinum_M AHFLSPMRAAIDMGLRPTNHTDFNVT-------PLDQMFVLWTAVNRVSR
TH_0643|M.thermoresistible__bu HHELWRLKSLLDAGIPTALSTDAPFG----DADPWAAMRAAVHRTTGSG-
* : .* * : .
MSMEG_0445|M.smegmatis_MC2_155 PEGGWTPH-EILPPEAALASYTGAVAYQAFAEKTWGRIVPGASADLVWLD
Mvan_1184|M.vanbaalenii_PYR-1 PPGGWTPH-EILPVERALTAYTAAVAHQAFAENTWGIIEPGFSADMVWLD
Mflv_2930|M.gilvum_PYR-GCK AAEVLSPADQKLTVEQAVHANTLGAAYQLRLDGLVGSVETGKRADLIVVD
MAB_2546|M.abscessus_ATCC_1997 GAQVLRPHDQKLSVAEAVRANTLGAAYQIRMDNKVGSVEVGKLADLIVLD
Mb0567|M.bovis_AF2122/97 --RVLAPE-ERLTVEQAIRAQTIDAAWQLFAEDAIGSLQVGKYADMVVLS
Rv0552|M.tuberculosis_H37Rv --RVLAPE-ERLTVEQAIRAQTIDAAWQLFAEDAIGSLQVGKYADMVVLS
MUL_0651|M.ulcerans_Agy99 --RVLAPE-ERLTVEQAIRAQTIDAAWQMFADDIIGSLEVGKYADMVVLS
MAV_4591|M.avium_104 --RVLAPE-ERLTVEQAIRAQTLDAAWQLFADDVIGSLEVGKYADLVVLS
MMAR_1131|M.marinum_M SGEVIGAD-QRVTALEALKAITINAAYQYSEEQSKGSITVGKLADLVIVD
TH_0643|M.thermoresistible__bu --AVLGAD-ERIDAGTALRMFFGTADRPTRPR----TVEPGQPADLCVLS
. : : *: . : * **: :.
MSMEG_0445|M.smegmatis_MC2_155 RDPRTVPPLELPAVGVRATYLQGRPAHTAAD----
Mvan_1184|M.vanbaalenii_PYR-1 RDPRLTPPLELPSTSVVGTYLRGTLASPS------
Mflv_2930|M.gilvum_PYR-GCK RNIFEIDPHDIHSAAVTLTMMDGEIRHRV------
MAB_2546|M.abscessus_ATCC_1997 RNILECDPQDIHTATVLMTMMNGQVRHELQGLKS-
Mb0567|M.bovis_AF2122/97 ADPRTVPPEQIADLAVRATFLAGRQVYRR------
Rv0552|M.tuberculosis_H37Rv ADPRTVPPEQIADLAVRATFLAGRQVYRR------
MUL_0651|M.ulcerans_Agy99 ADPRTVPPEEIADLEVRATFLAGRQVYRK------
MAV_4591|M.avium_104 ADPRAVPPERIADLQVRATYLAGRRVYPQ------
MMAR_1131|M.marinum_M NNPLTVDPMKLKDIAVLETIKEGRTIYREPATQSS
TH_0643|M.thermoresistible__bu APPDRVLR-ELSAELVSATIVGGKPVYQKPV----
: * * *