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EQLAEQVKMVFVYGLPAFLLLIAVEYYSYFRERRENTAPSEPKRGVSSRDTAANISTYLIRLVVSPLEKF IELPIIVIAAAFAPLALPADQWWTWALALFLADATYYVKHRMGHRIRLFWAEHSVHHSSQYFNLSTSLRL PWLVPGSFLKSVVSIPMALIGIPVWMIFLCQAIVLVYQFPIHTERVGRLPRPIEFIFNTPSHHRVHHGSN NPYLDKNYGGILIIFDRMFGSYAEELEPVRYGLTKNIDTNNPIKVNYHEFVLMLRDVWHAETWRGRIGYL LRPPGWQETKTDTAQPELVHTAS*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_0147 | - | - | 100% (304) | C-5 sterol desaturase |
| M. smegmatis MC2 155 | MSMEG_2812 | - | 3e-79 | 50.35% (282) | C-5 sterol desaturase |
| M. smegmatis MC2 155 | MSMEG_3666 | - | 2e-73 | 45.12% (297) | C-5 sterol desaturase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1844 | erg3 | 1e-67 | 44.76% (286) | membrane-bound C-5 sterol desaturase erg3 |
| M. gilvum PYR-GCK | Mflv_3418 | - | 5e-69 | 44.22% (303) | sterol desaturase-related protein |
| M. tuberculosis H37Rv | Rv1814 | erg3 | 1e-67 | 44.76% (286) | membrane-bound C-5 sterol desaturase erg3 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0067 | - | 2e-07 | 22.65% (234) | hypothetical protein MAB_0067 |
| M. marinum M | MMAR_3832 | erg3_2 | 1e-106 | 63.41% (276) | membrane-bound C-5 sterol desaturase Erg3_2 |
| M. avium 104 | MAV_2902 | - | 8e-68 | 44.76% (286) | C-5 sterol desaturase |
| M. thermoresistible (build 8) | TH_2226 | - | 8e-75 | 46.76% (293) | MEMBRANE-BOUND C-5 STEROL DESATURASE ERG3 |
| M. ulcerans Agy99 | MUL_3066 | erg3 | 5e-67 | 44.33% (291) | membrane-bound C-5 sterol desaturase Erg3 |
| M. vanbaalenii PYR-1 | Mvan_3171 | - | 3e-71 | 45.12% (297) | sterol desaturase-related protein |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_0147|M.smegmatis_MC2_155 --------MEQLAEQVKMVFVYGLPAFLLLIAVEYYSY-FRERRENT---
MMAR_3832|M.marinum_M ------------MERAVPIFLTG---FMVLVGLDFLSYRLGKQRNRS---
Mb1844|M.bovis_AF2122/97 ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETA---
Rv1814|M.tuberculosis_H37Rv ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETA---
MAV_2902|M.avium_104 -MSALSGYLTALPPQLRDPVLVAIPFFLLLLTLEWTAARKLEHLT-----
MUL_3066|M.ulcerans_Agy99 -MSALPGFLAVLPGEMRDPVLFAIPFFLFLLTVEWAAARKLEKLEAG---
Mflv_3418|M.gilvum_PYR-GCK -----MEFLEFLPPQMREPVLFAIPFFLLLLAIEWTAARKLEHAVEGGAD
Mvan_3171|M.vanbaalenii_PYR-1 -----MEFLDFLPPQMREPVLFAIPFFLLLLTIEWTAARKLEHTAP----
TH_2226|M.thermoresistible__bu VDQVVVPFLDVLPAPMRDPVLFAIPFFLLLLALEWTAARRLAHVEAGEGW
MAB_0067|M.abscessus_ATCC_1997 -MAIWFVRYGYVPLMLLGINGVAIALAHRPWAPLWMVVLILVAIGLSFLA
. :
MSMEG_0147|M.smegmatis_MC2_155 ---APSEPKRGVSSRDTAANISTYLIRLVVSPLEKFIELP-IIVIAAAFA
MMAR_3832|M.marinum_M ---RPS-----VSARDTAANLSVYALARLTRSVGSVTAVP-LVLIASALT
Mb1844|M.bovis_AF2122/97 ATGQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
Rv1814|M.tuberculosis_H37Rv ATGQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
MAV_2902|M.avium_104 ----ARPAPGAHRTRDSLTSISMGLVSVATTAGWKTLALFGYAAIYAYLA
MUL_3066|M.ulcerans_Agy99 ATETPRPVSGSYHTRDSVATISMGLVSLVTIAFWKTMALLGYAAIYTYVA
Mflv_3418|M.gilvum_PYR-GCK EDGT--PAAGAYHTRDSWASLSMGLVSTVTMSVWKFFALLGYAAIYAYLA
Mvan_3171|M.vanbaalenii_PYR-1 DGGS--PAVGAYHTRDSWASVSMGLVSTVTMSVWKFLALLGYAALYAYVA
TH_2226|M.thermoresistible__bu EEGDRRPPPGAFHTPDARASISMGLVSIATMGAWKFLALLGYVAIYAYLA
MAB_0067|M.abscessus_ATCC_1997 ERVAPYSPEWNESMGDARRDAAHAFVN-ETALLLTVLVVPAVALLSPFHT
*: : : . . : : . :
MSMEG_0147|M.smegmatis_MC2_155 PLALPADQWWTWALALFLADATYYVKHRMGHRIRLFWAEHSVHHSSQYFN
MMAR_3832|M.marinum_M PIHLPTAQWWTWACALVLTDLAYYGRHRMTHRIRVFWAAHHVHHSSQYLN
Mb1844|M.bovis_AF2122/97 PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSSEYFN
Rv1814|M.tuberculosis_H37Rv PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSSEYFN
MAV_2902|M.avium_104 PWHLPATRWYTWAIPILGVDLLYYAYHRIAHRVRLIWATHQAHHSSEYYN
MUL_3066|M.ulcerans_Agy99 PWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWATHQAHHSSEYFN
Mflv_3418|M.gilvum_PYR-GCK PWQLSPTQWYTWVIAIVGVDLLFYVYHRTAHRVRLVWATHQAHHSSRYFN
Mvan_3171|M.vanbaalenii_PYR-1 PWQLSATQWYTWVIALVGVDLLFYVYHRMAHRVRIVWATHQAHHSSRYFN
TH_2226|M.thermoresistible__bu PWHLPADRWYTWVIALVGVDLLFYVYHRMAHRVQVVWATHQAHHSSEYFN
MAB_0067|M.abscessus_ATCC_1997 WWPSSIPFVLQVLIAAAVADFGITLMHYASHKVPALWRLHAVHHSIKRFY
. . .* * *:: .* * .*** .
MSMEG_0147|M.smegmatis_MC2_155 LSTSLRLPWLVPG-SFLKSVVSIPMALIGIPVWMIFLCQAIVLVYQFPIH
MMAR_3832|M.marinum_M LSTGMRTPWLVPGWLFISAVVYVPLALIGFPVWMIFACHAIVLFYQFPIH
Mb1844|M.bovis_AF2122/97 FATALRQKWNNSG----EILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVH
Rv1814|M.tuberculosis_H37Rv FATALRQKWNNSG----EILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVH
MAV_2902|M.avium_104 FATALRQKWNNSG----EILMWIPLPLLGIPPWMVFFSFSLNLIYQFWIH
MUL_3066|M.ulcerans_Agy99 FATALRQKWNNSG----EVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVH
Mflv_3418|M.gilvum_PYR-GCK FATALRQKWNNSG----EILMWIPLPLLGVPPWMVFVAFSISLIYQFWIH
Mvan_3171|M.vanbaalenii_PYR-1 FATALRQKWNNSG----EILMWIPLPLLGVPPWMVFFAFSVSLIYQFWIH
TH_2226|M.thermoresistible__bu LATALRQKWNNSG----EVLLWIPLPLLGVPPWLVFFSFSISLIYQFWIH
MAB_0067|M.abscessus_ATCC_1997 GFNGLMKHPVHGG--LELMAGTAPLLIMGLP-QAVAEALTVLIAVQLLLQ
..: * *: ::*.* : :: : *: ::
MSMEG_0147|M.smegmatis_MC2_155 TERVGRLPRPIEFIFNTPSHHRVHHGSNNPYLDKNYGGILIIFDRMFGSY
MMAR_3832|M.marinum_M TERINRLPRPIEYLFNTPSHHRVHHGANNPYLNKNYGGILIVWDRLFGSY
Mb1844|M.bovis_AF2122/97 TERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSF
Rv1814|M.tuberculosis_H37Rv TERIDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSF
MAV_2902|M.avium_104 TERIDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTF
MUL_3066|M.ulcerans_Agy99 TERINKLPRWFEFIFNTPSHHRVHHGMDQMYLDKNYGGILIIWDRLFRSF
Mflv_3418|M.gilvum_PYR-GCK TEHIGKLWRPFEFVFNTPSHHRVHHGMDPLYLDKNYGGILIIWDRLFGTF
Mvan_3171|M.vanbaalenii_PYR-1 TERIGKLWLPVEFIFNTPSHHRVHHGMDKLYLDKNYGGILIIWDRLFGTF
TH_2226|M.thermoresistible__bu TERIGKLPAPIEFVFNTPSHHRVHHGMDPEYLDKNYGGILIIWDRLFGTF
MAB_0067|M.abscessus_ATCC_1997 HSNADYRIGRLRVVLALNEGHRFHHLKWAGIGDVNFGLFTLVWDHLLRTF
.. . .. :: . **.** : *:* : :::*::: ::
MSMEG_0147|M.smegmatis_MC2_155 AEELEPVRYGLTKNIDTNNPIKVNYHEFVLMLRDVWHAETWRGRIGYLLR
MMAR_3832|M.marinum_M AEEDEPVRYGLQQNIATHNPIKLNYHEFVAMLSDVGRAKTWRGRVGYLLR
Mb1844|M.bovis_AF2122/97 QPELFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFG
Rv1814|M.tuberculosis_H37Rv QPELFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFG
MAV_2902|M.avium_104 QAELFRPHYGLTKQVDTFNVWKLQTREYVAIARDWRSASRLRDRLGYVFG
MUL_3066|M.ulcerans_Agy99 QAETARPHYGLTKPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFG
Mflv_3418|M.gilvum_PYR-GCK QPEVFRPHYGLTKPVDTFNIWKLQTHEYAAIWRDARAARGVRAKLGYIFG
Mvan_3171|M.vanbaalenii_PYR-1 QPEVFRPHYGLTKPVDTFNIWKLQTHEYVAIGRDVRAARGLRAKLGYIFG
TH_2226|M.thermoresistible__bu CEEKFRPRYGLTKPVDTYNIWRLQTHEYLAIARDVAKARNWRERLGYIFG
MAB_0067|M.abscessus_ATCC_1997 SFDPARRFHSDDLGMAAKPNYPAGYTAQLAEPFRTSGACHFDG-LQSEVT
: :. : : * : .
MSMEG_0147|M.smegmatis_MC2_155 PPGWQETKTDTAQP-----------ELVHTAS----
MMAR_3832|M.marinum_M PPGWSEVAETPAPRSHDSDDHAVPVDGQHWAAAGNG
Mb1844|M.bovis_AF2122/97 PPGWEPRTID--KSNAAASLVTSR------------
Rv1814|M.tuberculosis_H37Rv PPGWEPRTID--KSNAAASLVTSR------------
MAV_2902|M.avium_104 PPGWAPRSAG--RTAAGAPVVTSL------------
MUL_3066|M.ulcerans_Agy99 PPGWQPSTTATPRAEDAATVTTSGWRPT--------
Mflv_3418|M.gilvum_PYR-GCK PPGWQP---AQVQTPAPVAP----------------
Mvan_3171|M.vanbaalenii_PYR-1 PPGWEPSTAPGARAAAHATP----------------
TH_2226|M.thermoresistible__bu PPGWQPAP-AGAPTPAPARD----------------
MAB_0067|M.abscessus_ATCC_1997 ATGRQPST----------------------------
..*