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MRDPVLFAIPCFLLLLILEWTAARKLESIETAATGQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLA LLGYAAIYAYLAPWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSSEYFNFATALRQK WNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTERIDRLPRWFEFVFNTPSHHRVHHGMDPVYL DKNYGGILIIWDRLFGSFQPELFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPP GWEPRTIDKSNAAASLVTSR
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. tuberculosis H37Rv | Rv1814 | erg3 | - | 100% (300) | MEMBRANE-BOUND C-5 STEROL DESATURASE ERG3 (STEROL-C5-DESATURASE) |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1844 | erg3 | 0.0 | 100.00% (300) | membrane-bound C-5 sterol desaturase erg3 |
| M. gilvum PYR-GCK | Mflv_3418 | - | 1e-135 | 77.08% (288) | sterol desaturase-related protein |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0067 | - | 4e-07 | 28.15% (135) | hypothetical protein MAB_0067 |
| M. marinum M | MMAR_2691 | erg3 | 1e-149 | 81.40% (301) | membrane-bound C-5 sterol desaturase Erg3 |
| M. avium 104 | MAV_2902 | - | 1e-149 | 81.27% (299) | C-5 sterol desaturase |
| M. smegmatis MC2 155 | MSMEG_3666 | - | 1e-133 | 76.41% (284) | C-5 sterol desaturase |
| M. thermoresistible (build 8) | TH_2226 | - | 1e-130 | 73.87% (287) | MEMBRANE-BOUND C-5 STEROL DESATURASE ERG3 |
| M. ulcerans Agy99 | MUL_3066 | erg3 | 1e-149 | 80.40% (301) | membrane-bound C-5 sterol desaturase Erg3 |
| M. vanbaalenii PYR-1 | Mvan_3171 | - | 1e-135 | 76.53% (294) | sterol desaturase-related protein |
CLUSTAL 2.0.9 multiple sequence alignment
Rv1814|M.tuberculosis_H37Rv ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETAAT-
Mb1844|M.bovis_AF2122/97 ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETAAT-
MMAR_2691|M.marinum_M ---------------MRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGAT-
MUL_3066|M.ulcerans_Agy99 -MSALPGFLAVLPGEMRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGAT-
MAV_2902|M.avium_104 -MSALSGYLTALPPQLRDPVLVAIPFFLLLLTLEWTAARKLEHLTA----
MSMEG_3666|M.smegmatis_MC2_155 -MDTLRSVLEFLPPPMRDPVMFAIPFFLLLLTIEWTAARRLEHVDPAE--
TH_2226|M.thermoresistible__bu VDQVVVPFLDVLPAPMRDPVLFAIPFFLLLLALEWTAARRLAHVEAGEGW
Mflv_3418|M.gilvum_PYR-GCK -----MEFLEFLPPQMREPVLFAIPFFLLLLAIEWTAARKLEHAVEGGA-
Mvan_3171|M.vanbaalenii_PYR-1 -----MEFLDFLPPQMREPVLFAIPFFLLLLTIEWTAARKLEHTAP----
MAB_0067|M.abscessus_ATCC_1997 -MAIWFVRYGYVPLMLLGINGVAIALAHRPWAPLWMVVLILVAIGLSFLA
: .**. * .. *
Rv1814|M.tuberculosis_H37Rv --GQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
Mb1844|M.bovis_AF2122/97 --GQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
MMAR_2691|M.marinum_M --ETPRPASGSYHTRDSVASISMGLVSLVTIAFWKTLALLGYAAIYTYVA
MUL_3066|M.ulcerans_Agy99 --ETPRPVSGSYHTRDSVATISMGLVSLVTIAFWKTMALLGYAAIYTYVA
MAV_2902|M.avium_104 -----RPAPGAHRTRDSLTSISMGLVSVATTAGWKTLALFGYAAIYAYLA
MSMEG_3666|M.smegmatis_MC2_155 --GDR-APAGAFHTRDAWTSVSMGLVSVATTAGWKLLALLGYAVLYAYVA
TH_2226|M.thermoresistible__bu EEGDRRPPPGAFHTPDARASISMGLVSIATMGAWKFLALLGYVAIYAYLA
Mflv_3418|M.gilvum_PYR-GCK -DEDGTPAAGAYHTRDSWASLSMGLVSTVTMSVWKFFALLGYAAIYAYLA
Mvan_3171|M.vanbaalenii_PYR-1 --DGGSPAVGAYHTRDSWASVSMGLVSTVTMSVWKFLALLGYAALYAYVA
MAB_0067|M.abscessus_ATCC_1997 ERVAPYSPEWNESMGDARRDAAHAFVN-ETALLLTVLVVPAVALLSPFHT
. *: : .:*. * . :.: . . : .: :
Rv1814|M.tuberculosis_H37Rv PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSS-EYF
Mb1844|M.bovis_AF2122/97 PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSS-EYF
MMAR_2691|M.marinum_M PWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWATHQAHHSS-EYF
MUL_3066|M.ulcerans_Agy99 PWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWATHQAHHSS-EYF
MAV_2902|M.avium_104 PWHLPATRWYTWAIPILGVDLLYYAYHRIAHRVRLIWATHQAHHSS-EYY
MSMEG_3666|M.smegmatis_MC2_155 PWQLPADKWYTWVIALLGVDLLWYTYHRVAHRVRLIWATHQAHHSS-EYF
TH_2226|M.thermoresistible__bu PWHLPADRWYTWVIALVGVDLLFYVYHRMAHRVQVVWATHQAHHSS-EYF
Mflv_3418|M.gilvum_PYR-GCK PWQLSPTQWYTWVIAIVGVDLLFYVYHRTAHRVRLVWATHQAHHSS-RYF
Mvan_3171|M.vanbaalenii_PYR-1 PWQLSATQWYTWVIALVGVDLLFYVYHRMAHRVRIVWATHQAHHSS-RYF
MAB_0067|M.abscessus_ATCC_1997 WWPSSIPFVLQVLIAAAVADFGITLMHYASHKVPALWRLHAVHHSIKRFY
* . *. .*: * :*:* :* * .*** .::
Rv1814|M.tuberculosis_H37Rv NFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTER
Mb1844|M.bovis_AF2122/97 NFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTER
MMAR_2691|M.marinum_M NFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTER
MUL_3066|M.ulcerans_Agy99 NFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTER
MAV_2902|M.avium_104 NFATALRQKWNNSGEILMWIPLPLLGIPPWMVFFSFSLNLIYQFWIHTER
MSMEG_3666|M.smegmatis_MC2_155 NFATALRQKWNNSGEIVAWIPLPLLGIPPWVVFAAFSVNLIYQFWVHTER
TH_2226|M.thermoresistible__bu NLATALRQKWNNSGEVLLWIPLPLLGVPPWLVFFSFSISLIYQFWIHTER
Mflv_3418|M.gilvum_PYR-GCK NFATALRQKWNNSGEILMWIPLPLLGVPPWMVFVAFSISLIYQFWIHTEH
Mvan_3171|M.vanbaalenii_PYR-1 NFATALRQKWNNSGEILMWIPLPLLGVPPWMVFFAFSVSLIYQFWIHTER
MAB_0067|M.abscessus_ATCC_1997 GFNGLMKHPVHGGLELMAGTAPLLIMGLPQAVAEALTVLIAVQLLLQHSN
.: ::: :.. *:: . *: * * . :: : *: :: ..
Rv1814|M.tuberculosis_H37Rv IDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPE
Mb1844|M.bovis_AF2122/97 IDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPE
MMAR_2691|M.marinum_M INKLPRWFEFIFNTPSHHRVHHGMDHMYLDKNYGGILIIWDRLFRSFQAE
MUL_3066|M.ulcerans_Agy99 INKLPRWFEFIFNTPSHHRVHHGMDQMYLDKNYGGILIIWDRLFRSFQAE
MAV_2902|M.avium_104 IDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTFQAE
MSMEG_3666|M.smegmatis_MC2_155 IGKLPRPIEFVFNTPSHHRVHHGSDELYLDRNYGGILIIWDRLFGTFQEE
TH_2226|M.thermoresistible__bu IGKLPAPIEFVFNTPSHHRVHHGMDPEYLDKNYGGILIIWDRLFGTFCEE
Mflv_3418|M.gilvum_PYR-GCK IGKLWRPFEFVFNTPSHHRVHHGMDPLYLDKNYGGILIIWDRLFGTFQPE
Mvan_3171|M.vanbaalenii_PYR-1 IGKLWLPVEFIFNTPSHHRVHHGMDKLYLDKNYGGILIIWDRLFGTFQPE
MAB_0067|M.abscessus_ATCC_1997 ADYRIGRLRVVLALNEGHRFHHLKWAGIGDVNFGLFTLVWDHLLRTFSFD
. ...:: . **.** * *:* : ::**:*: :* :
Rv1814|M.tuberculosis_H37Rv LFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPG
Mb1844|M.bovis_AF2122/97 LFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPG
MMAR_2691|M.marinum_M TARPHYGLTKPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPG
MUL_3066|M.ulcerans_Agy99 TARPHYGLTKPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPG
MAV_2902|M.avium_104 LFRPHYGLTKQVDTFNVWKLQTREYVAIARDWRSASRLRDRLGYVFGPPG
MSMEG_3666|M.smegmatis_MC2_155 VFRPRYGLTKPVGTYNIWKLQTHEYVAIAHDVRSARRWTDRLGYVFGPPG
TH_2226|M.thermoresistible__bu KFRPRYGLTKPVDTYNIWRLQTHEYLAIARDVAKARNWRERLGYIFGPPG
Mflv_3418|M.gilvum_PYR-GCK VFRPHYGLTKPVDTFNIWKLQTHEYAAIWRDARAARGVRAKLGYIFGPPG
Mvan_3171|M.vanbaalenii_PYR-1 VFRPHYGLTKPVDTFNIWKLQTHEYVAIGRDVRAARGLRAKLGYIFGPPG
MAB_0067|M.abscessus_ATCC_1997 PARRFHSDDLGMAAKPNYPAGYTAQLAEPFRTSGACHFDG-LQSEVTATG
* :. : : : * * * . ..*
Rv1814|M.tuberculosis_H37Rv WEPRTID--KSNAAASLVTSR----
Mb1844|M.bovis_AF2122/97 WEPRTID--KSNAAASLVTSR----
MMAR_2691|M.marinum_M WQPSTTATPRAEDAAAVTTSG----
MUL_3066|M.ulcerans_Agy99 WQPSTTATPRAEDAATVTTSGWRPT
MAV_2902|M.avium_104 WAPRSAG--RTAAGAPVVTSL----
MSMEG_3666|M.smegmatis_MC2_155 WTPAR-TCETTEVTT----------
TH_2226|M.thermoresistible__bu WQPAP-AGAPTPAPARD--------
Mflv_3418|M.gilvum_PYR-GCK WQP---AQVQTPAPVAP--------
Mvan_3171|M.vanbaalenii_PYR-1 WEPSTAPGARAAAHATP--------
MAB_0067|M.abscessus_ATCC_1997 RQPST--------------------
*