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DTLRSVLEFLPPPMRDPVMFAIPFFLLLLTIEWTAARRLEHVDPAEGDRAPAGAFHTRDAWTSVSMGLVS VATTAGWKLLALLGYAVLYAYVAPWQLPADKWYTWVIALLGVDLLWYTYHRVAHRVRLIWATHQAHHSSE YFNFATALRQKWNNSGEIVAWIPLPLLGIPPWVVFAAFSVNLIYQFWVHTERIGKLPRPIEFVFNTPSHH RVHHGSDELYLDRNYGGILIIWDRLFGTFQEEVFRPRYGLTKPVGTYNIWKLQTHEYVAIAHDVRSARRW TDRLGYVFGPPGWTPARTCETTEVTT*
Operon Prediction Model: Genebank
Paralogs
| species | id | gene | e-value | identity (len) | annotation |
| M. smegmatis MC2 155 | MSMEG_3666 | - | - | 100% (307) | C-5 sterol desaturase |
| M. smegmatis MC2 155 | MSMEG_0147 | - | 2e-73 | 45.12% (297) | C-5 sterol desaturase |
| M. smegmatis MC2 155 | MSMEG_2812 | - | 6e-61 | 42.55% (282) | C-5 sterol desaturase |
Closest Orthologs (e-value cutoff: 1e-4)
| species | id | gene | e-value | identity (len) | annotation |
| M. bovis AF2122 / 97 | Mb1844 | erg3 | 1e-133 | 76.41% (284) | membrane-bound C-5 sterol desaturase erg3 |
| M. gilvum PYR-GCK | Mflv_3418 | - | 1e-139 | 75.85% (294) | sterol desaturase-related protein |
| M. tuberculosis H37Rv | Rv1814 | erg3 | 1e-133 | 76.41% (284) | membrane-bound C-5 sterol desaturase erg3 |
| M. leprae Br4923 | - | - | - | - | - |
| M. abscessus ATCC 19977 | MAB_0067 | - | 6e-08 | 28.99% (138) | hypothetical protein MAB_0067 |
| M. marinum M | MMAR_2691 | erg3 | 1e-132 | 73.87% (287) | membrane-bound C-5 sterol desaturase Erg3 |
| M. avium 104 | MAV_2902 | - | 1e-139 | 73.55% (310) | C-5 sterol desaturase |
| M. thermoresistible (build 8) | TH_2226 | - | 1e-143 | 76.61% (295) | MEMBRANE-BOUND C-5 STEROL DESATURASE ERG3 |
| M. ulcerans Agy99 | MUL_3066 | erg3 | 1e-133 | 71.10% (301) | membrane-bound C-5 sterol desaturase Erg3 |
| M. vanbaalenii PYR-1 | Mvan_3171 | - | 1e-143 | 78.62% (290) | sterol desaturase-related protein |
CLUSTAL 2.0.9 multiple sequence alignment
MSMEG_3666|M.smegmatis_MC2_155 -MDTLRSVLEFLPPPMRDPVMFAIPFFLLLLTIEWTAARRLEHVDPAE--
TH_2226|M.thermoresistible__bu VDQVVVPFLDVLPAPMRDPVLFAIPFFLLLLALEWTAARRLAHVEAGEGW
Mflv_3418|M.gilvum_PYR-GCK -----MEFLEFLPPQMREPVLFAIPFFLLLLAIEWTAARKLEHAVEGGA-
Mvan_3171|M.vanbaalenii_PYR-1 -----MEFLDFLPPQMREPVLFAIPFFLLLLTIEWTAARKLEHTAP----
Mb1844|M.bovis_AF2122/97 ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETAAT-
Rv1814|M.tuberculosis_H37Rv ---------------MRDPVLFAIPCFLLLLILEWTAARKLESIETAAT-
MMAR_2691|M.marinum_M ---------------MRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGAT-
MUL_3066|M.ulcerans_Agy99 -MSALPGFLAVLPGEMRDPVLFAIPFFLFLLTVEWAAARKLEKLEAGAT-
MAV_2902|M.avium_104 -MSALSGYLTALPPQLRDPVLVAIPFFLLLLTLEWTAARKLEHLTA----
MAB_0067|M.abscessus_ATCC_1997 -MAIWFVRYGYVPLMLLGINGVAIALAHRPWAPLWMVVLILVAIGLSFLA
: .**. * .. *
MSMEG_3666|M.smegmatis_MC2_155 --GDR-APAGAFHTRDAWTSVSMGLVSVATTAGWKLLALLGYAVLYAYVA
TH_2226|M.thermoresistible__bu EEGDRRPPPGAFHTPDARASISMGLVSIATMGAWKFLALLGYVAIYAYLA
Mflv_3418|M.gilvum_PYR-GCK -DEDGTPAAGAYHTRDSWASLSMGLVSTVTMSVWKFFALLGYAAIYAYLA
Mvan_3171|M.vanbaalenii_PYR-1 --DGGSPAVGAYHTRDSWASVSMGLVSTVTMSVWKFLALLGYAALYAYVA
Mb1844|M.bovis_AF2122/97 --GQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
Rv1814|M.tuberculosis_H37Rv --GQPRPASGAYLTRDSVASISMGLVSIATTAGWKSLALLGYAAIYAYLA
MMAR_2691|M.marinum_M --ETPRPASGSYHTRDSVASISMGLVSLVTIAFWKTLALLGYAAIYTYVA
MUL_3066|M.ulcerans_Agy99 --ETPRPVSGSYHTRDSVATISMGLVSLVTIAFWKTMALLGYAAIYTYVA
MAV_2902|M.avium_104 -----RPAPGAHRTRDSLTSISMGLVSVATTAGWKTLALFGYAAIYAYLA
MAB_0067|M.abscessus_ATCC_1997 ERVAPYSPEWNESMGDARRDAAHAFVN-ETALLLTVLVVPAVALLSPFHT
. *: : .:*. * . :.: . . : .: :
MSMEG_3666|M.smegmatis_MC2_155 PWQLPADKWYTWVIALLGVDLLWYTYHRVAHRVRLIWATHQAHHSS-EYF
TH_2226|M.thermoresistible__bu PWHLPADRWYTWVIALVGVDLLFYVYHRMAHRVQVVWATHQAHHSS-EYF
Mflv_3418|M.gilvum_PYR-GCK PWQLSPTQWYTWVIAIVGVDLLFYVYHRTAHRVRLVWATHQAHHSS-RYF
Mvan_3171|M.vanbaalenii_PYR-1 PWQLSATQWYTWVIALVGVDLLFYVYHRMAHRVRIVWATHQAHHSS-RYF
Mb1844|M.bovis_AF2122/97 PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSS-EYF
Rv1814|M.tuberculosis_H37Rv PWQLSAHRWYTWVIAIVGVDLLYYSYHRIAHRVRLIWATHQAHHSS-EYF
MMAR_2691|M.marinum_M PWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWATHQAHHSS-EYF
MUL_3066|M.ulcerans_Agy99 PWHLSPGRWYTWVIAIVGVDLLYYTYHRIAHRVRLIWATHQAHHSS-EYF
MAV_2902|M.avium_104 PWHLPATRWYTWAIPILGVDLLYYAYHRIAHRVRLIWATHQAHHSS-EYY
MAB_0067|M.abscessus_ATCC_1997 WWPSSIPFVLQVLIAAAVADFGITLMHYASHKVPALWRLHAVHHSIKRFY
* . *. .*: * :*:* :* * .*** .::
MSMEG_3666|M.smegmatis_MC2_155 NFATALRQKWNNSGEIVAWIPLPLLGIPPWVVFAAFSVNLIYQFWVHTER
TH_2226|M.thermoresistible__bu NLATALRQKWNNSGEVLLWIPLPLLGVPPWLVFFSFSISLIYQFWIHTER
Mflv_3418|M.gilvum_PYR-GCK NFATALRQKWNNSGEILMWIPLPLLGVPPWMVFVAFSISLIYQFWIHTEH
Mvan_3171|M.vanbaalenii_PYR-1 NFATALRQKWNNSGEILMWIPLPLLGVPPWMVFFAFSVSLIYQFWIHTER
Mb1844|M.bovis_AF2122/97 NFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTER
Rv1814|M.tuberculosis_H37Rv NFATALRQKWNNSGEILMWVPLPLMGLPPWMVFCSWSLNLIYQFWVHTER
MMAR_2691|M.marinum_M NFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTER
MUL_3066|M.ulcerans_Agy99 NFATALRQKWNNSGEVLMWVPLPLLGLPPWMVFFGFSVSLIYQFWVHTER
MAV_2902|M.avium_104 NFATALRQKWNNSGEILMWIPLPLLGIPPWMVFFSFSLNLIYQFWIHTER
MAB_0067|M.abscessus_ATCC_1997 GFNGLMKHPVHGGLELMAGTAPLLIMGLPQAVAEALTVLIAVQLLLQHSN
.: ::: :.. *:: . *: * * . :: : *: :: ..
MSMEG_3666|M.smegmatis_MC2_155 IGKLPRPIEFVFNTPSHHRVHHGSDELYLDRNYGGILIIWDRLFGTFQEE
TH_2226|M.thermoresistible__bu IGKLPAPIEFVFNTPSHHRVHHGMDPEYLDKNYGGILIIWDRLFGTFCEE
Mflv_3418|M.gilvum_PYR-GCK IGKLWRPFEFVFNTPSHHRVHHGMDPLYLDKNYGGILIIWDRLFGTFQPE
Mvan_3171|M.vanbaalenii_PYR-1 IGKLWLPVEFIFNTPSHHRVHHGMDKLYLDKNYGGILIIWDRLFGTFQPE
Mb1844|M.bovis_AF2122/97 IDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPE
Rv1814|M.tuberculosis_H37Rv IDRLPRWFEFVFNTPSHHRVHHGMDPVYLDKNYGGILIIWDRLFGSFQPE
MMAR_2691|M.marinum_M INKLPRWFEFIFNTPSHHRVHHGMDHMYLDKNYGGILIIWDRLFRSFQAE
MUL_3066|M.ulcerans_Agy99 INKLPRWFEFIFNTPSHHRVHHGMDQMYLDKNYGGILIIWDRLFRSFQAE
MAV_2902|M.avium_104 IDKLPRPFEFVFNTPSHHRVHHGMDKVYLDKNYGGILIVWDRLFGTFQAE
MAB_0067|M.abscessus_ATCC_1997 ADYRIGRLRVVLALNEGHRFHHLKWAGIGDVNFGLFTLVWDHLLRTFSFD
. ...:: . **.** * *:* : ::**:*: :* :
MSMEG_3666|M.smegmatis_MC2_155 VFRPRYGLTKPVGTYNIWKLQTHEYVAIAHDVRSARRWTDRLGYVFGPPG
TH_2226|M.thermoresistible__bu KFRPRYGLTKPVDTYNIWRLQTHEYLAIARDVAKARNWRERLGYIFGPPG
Mflv_3418|M.gilvum_PYR-GCK VFRPHYGLTKPVDTFNIWKLQTHEYAAIWRDARAARGVRAKLGYIFGPPG
Mvan_3171|M.vanbaalenii_PYR-1 VFRPHYGLTKPVDTFNIWKLQTHEYVAIGRDVRAARGLRAKLGYIFGPPG
Mb1844|M.bovis_AF2122/97 LFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPG
Rv1814|M.tuberculosis_H37Rv LFRPHYGLTKRVDTFNIWKLQTREYVAIVRDWRSATRLRDRLGYVFGPPG
MMAR_2691|M.marinum_M TARPHYGLTKPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPG
MUL_3066|M.ulcerans_Agy99 TARPHYGLTKPVDTFNIWKLQTREYVAIARDWRSATRLRDRLGYAFGPPG
MAV_2902|M.avium_104 LFRPHYGLTKQVDTFNVWKLQTREYVAIARDWRSASRLRDRLGYVFGPPG
MAB_0067|M.abscessus_ATCC_1997 PARRFHSDDLGMAAKPNYPAGYTAQLAEPFRTSGACHFDG-LQSEVTATG
* :. : : : * * * . ..*
MSMEG_3666|M.smegmatis_MC2_155 WTPAR-TCETTEVTT----------
TH_2226|M.thermoresistible__bu WQPAP-AGAPTPAPARD--------
Mflv_3418|M.gilvum_PYR-GCK WQP---AQVQTPAPVAP--------
Mvan_3171|M.vanbaalenii_PYR-1 WEPSTAPGARAAAHATP--------
Mb1844|M.bovis_AF2122/97 WEPRTID--KSNAAASLVTSR----
Rv1814|M.tuberculosis_H37Rv WEPRTID--KSNAAASLVTSR----
MMAR_2691|M.marinum_M WQPSTTATPRAEDAAAVTTSG----
MUL_3066|M.ulcerans_Agy99 WQPSTTATPRAEDAATVTTSGWRPT
MAV_2902|M.avium_104 WAPRSAG--RTAAGAPVVTSL----
MAB_0067|M.abscessus_ATCC_1997 RQPST--------------------
*