For questions or suggestions e-mail us at: ioerger@cs.tamu.edu

M. tuberculosis H37Rv Rv3704c (gshA)

annotation: GLUTAMATE--CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE SYNTHETASE) (GAMMA-ECS) (GCS) (GAMMA-GLUTAMYL-L-CYSTEINE SYNTHETASE)
coordinates: 4146888 - 4148186
length: 432

TLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITE
VLEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALANAGLGLVFLGADPLRSPVR
VNPGARYRAMEQFFAASHSGVPGAAMMTSTAAIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLG
GRFSGWQSTRQRVWGQMDSARCGPILGASGDHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWV
DGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLDSVPDEVWPAVVFTLVTLLDDPVAADLAVDAV
EPVATAWDTAARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGIA
SAVTGMMHGAS*

Operon Prediction Model: Genebank

Paralogs

species id gene e-value identity (len) annotation
M. tuberculosis H37Rv Rv3704c gshA - 100% (432) GLUTAMATE--CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE SYNTHETASE) (GAMMA-ECS) (GCS) (GAMMA-GLUTAMYL-L-CYSTEINE SYNTHETASE)

Closest Orthologs (e-value cutoff: 1e-4)

species id gene e-value identity (len) annotation
M. bovis AF2122 / 97 Mb3730c gshA 0.0 100.00% (432) glutamate--cysteine ligase gshA (gamma-glutamylcysteine
M. gilvum PYR-GCK Mflv_1315 - 1e-155 65.61% (410) glutamate--cysteine ligase, GCS2
M. leprae Br4923 - - - - -
M. abscessus ATCC 19977 MAB_0369 - 1e-107 49.75% (398) glutamate--cysteine ligase GshA
M. marinum M MMAR_5215 gshA 0.0 72.56% (430) glutamate--cysteine ligase GshA
M. avium 104 MAV_0399 - 0.0 73.73% (434) putative glutamate--cysteine ligase
M. smegmatis MC2 155 MSMEG_6250 - 1e-157 64.54% (423) glutamate--cysteine ligase
M. thermoresistible (build 8) TH_0772 gshA 1e-160 65.26% (426) GLUTAMATE--CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE
M. ulcerans Agy99 - - - - -
M. vanbaalenii PYR-1 Mvan_5486 - 1e-160 67.65% (408) glutamate--cysteine ligase, GCS2

CLUSTAL 2.0.9 multiple sequence alignment


Mflv_1315|M.gilvum_PYR-GCK          MNLSVLSGRWVKTQRPFHTRRGWLMAHTMASDGVVEHGV---------VP
Mvan_5486|M.vanbaalenii_PYR-1       ------------------------MACTMTSDGVVEQRF---------DP
MSMEG_6250|M.smegmatis_MC2_155      ------------------------MALPARSDSGCAVPV---------E-
TH_0772|M.thermoresistible__bu      ------------------------MTLPVTAEAPAT--------------
Rv3704c|M.tuberculosis_H37Rv        ------------------------MTLAAMTAAASQLDNAAPDD----VE
Mb3730c|M.bovis_AF2122/97           ------------------------MTLAAMTAAASQLDNAAPDD----VE
MMAR_5215|M.marinum_M               ------------------------MTFAPSPAATSQLDSSRAAD----AD
MAV_0399|M.avium_104                ------------------------MTFAPSTAAASRPAGGDPAAELRIAE
MAB_0369|M.abscessus_ATCC_1997      ------------------------MATTATEADTGR-------------T
                                                            *: .                      

Mflv_1315|M.gilvum_PYR-GCK          LTSAEDAAQHIVDTCLKDVPPGPVGLEIEAHCYDLSDPFRRPGWDELTAV
Mvan_5486|M.vanbaalenii_PYR-1       LSSAEAAAQHIVDGCLIDGPVGQVGLEVEAHCFDLTDPSRRPGWDELTAT
MSMEG_6250|M.smegmatis_MC2_155      FTSAEQAAAHIGANSLQDGPIGRVGLEIEAHCFDLSNPTRRPSWDELSAV
TH_0772|M.thermoresistible__bu      VDSARRAAAHIEASCLRDGPVGRVGLEIEAHCFDVADPGRRPGWAELTDV
Rv3704c|M.tuberculosis_H37Rv        ITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEV
Mb3730c|M.bovis_AF2122/97           ITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEV
MMAR_5215|M.marinum_M               IAGSAVAAQYIANGCLVDGPIGRVGLEIEAHCYDPADPHRRPSWEEIGDV
MAV_0399|M.avium_104                LTDSAAVAHYIARGCLADAPLGRVGLEVEAHCHDPQDPCRRPGWDEIAAV
MAB_0369|M.abscessus_ATCC_1997      LSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRLREV
                                    . .   .* :*   .: *   * ****:*:*  :   * **  * .:  .

Mflv_1315|M.gilvum_PYR-GCK          IADLPRLPGGSLVTVEPGGAVELSGPPATGTADAVSAMVTDRAVLRDVFA
Mvan_5486|M.vanbaalenii_PYR-1       ITALPPLPGGSVVTVEPGGAVELSGPPLAEPASAISAMAADHTVLRAAFA
MSMEG_6250|M.smegmatis_MC2_155      IADVPPLPGGSRITVEPGGAVELSGPPYDGPLAAVAALQADRAVLRAEFA
TH_0772|M.thermoresistible__bu      IATVGELPGGSAISVEPGGAVELSGPPLDGPVAAATAMLTDQAVLRAAFA
Rv3704c|M.tuberculosis_H37Rv        LEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALA
Mb3730c|M.bovis_AF2122/97           LEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALA
MMAR_5215|M.marinum_M               LEGLGKPPGGSAVTVEPGGAVELSGPPCDGILAAIDAMHRDQAVLRSAFA
MAV_0399|M.avium_104                LDALPALPGGSRVTVEPGGAVELSGPPADGVLAAIDAMRRDQAVLRPAFA
MAB_0369|M.abscessus_ATCC_1997      GDSVPDLPGSSAITFEPGGAVELSGPPCSDVWSAISSMRADHEILTAVYR
                                       :   **.* ::.************      *  ::  *: :*     

Mflv_1315|M.gilvum_PYR-GCK          GAGLGLVLLGADPLRPAGRVNPGSRYAAMERFFAESGTPEAGAAMMTSTA
Mvan_5486|M.vanbaalenii_PYR-1       RAGLGLVLVGADPLRPAARVNPGPRYQAMEAFFRASDSGDAGAAMMTSTA
MSMEG_6250|M.smegmatis_MC2_155      RRNLGLVLLGTDPLRPTRRVNPGARYSAMEQFFTASGTAEAGAAMMTATA
TH_0772|M.thermoresistible__bu      RAGYALVMLGADPVRQPQRVNPGSRYRAMETFFAASGTAAAGSAMMTSTA
Rv3704c|M.tuberculosis_H37Rv        NAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTA
Mb3730c|M.bovis_AF2122/97           NAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTA
MMAR_5215|M.marinum_M               DAGLGLVCLGADPIRPTMRINPGARYQAMEQFFTASQSGMAGAAMMTSTA
MAV_0399|M.avium_104                EAGLGLVFLGADPLRPPQRINPGARYRAMERFFATSRSGEAGAAMMTSTA
MAB_0369|M.abscessus_ATCC_1997      GAGIALASLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMTCTA
                                      . .*. :*:**:* . *:***.** *** .*  :    ..  ***.**

Mflv_1315|M.gilvum_PYR-GCK          SVQVNLEAGHRAGWADRVRLAHALGPTMIAIAANSPVLAGKFTGWHSTRQ
Mvan_5486|M.vanbaalenii_PYR-1       SVQVNLEAGPRAGWADRVRLAHALGPTMIAIAANSPVLGGDFTGWQSTRQ
MSMEG_6250|M.smegmatis_MC2_155      SVQVNLDAGPRDGWAERVRLAHALGPTMIAITANSPMLGGQFTGWCSTRQ
TH_0772|M.thermoresistible__bu      SIQVNVEAGPRTGWAERIRLAHALGPVMVAIAANSPLLGGRFSGWRSSRQ
Rv3704c|M.tuberculosis_H37Rv        AIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQ
Mb3730c|M.bovis_AF2122/97           AIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQ
MMAR_5215|M.marinum_M               SIQVNLDAGPQSGWASRVRLAHALGPTMIAIAANSPMLGGEFSGWRSTRQ
MAV_0399|M.avium_104                SIQVNVDAGPREGWAARVRLAHALGPTMIAMTANSPMLDGDFTGWVSSRQ
MAB_0369|M.abscessus_ATCC_1997      SLQVNVQSGTPRQWRDRFVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQ
                                    ::***:::*    *  *. **: :**.* *::*.**:* *  :*  .:**

Mflv_1315|M.gilvum_PYR-GCK          LVWSQLDSARCGPILG-VDGD-DPASDWARYALRAPVMLVHTP---EAVP
Mvan_5486|M.vanbaalenii_PYR-1       LVWSRLDSARCGPILG-ASGD-DPASDWARYALRAPVMLVHSP---DPTP
MSMEG_6250|M.smegmatis_MC2_155      RVWGQLDSARCGPVLG-VDGD-DPASEWARYALRAPVMLVNSP---DAVP
TH_0772|M.thermoresistible__bu      RIWGQLDSARCGPILGGRDGVRTPGGDWARYALRAPVMLVNGAGDRTAEP
Rv3704c|M.tuberculosis_H37Rv        RVWGQMDSARCGPILG-ASGD-HPGIDWAKYALKAPVMMVRSPDTQDTRA
Mb3730c|M.bovis_AF2122/97           RVWGQMDSARCGPILG-ASGD-HPGIDWAKYALKAPVMMVRSPDTQDTRA
MMAR_5215|M.marinum_M               RVWGQMDDGRCGPILG-ASAD-DPATDWAHYALKAPVMLVHNP---DAVA
MAV_0399|M.avium_104                RVWGRMDSARCGPILG-ASGD-DPGTDWARYALKAPVMLVHTP---DAEA
MAB_0369|M.abscessus_ATCC_1997      WIWDNLDPRRCAPVEI----GVDPTESWVEYALRAPVMLVRNKD--GADA
                                     :*..:*  **.*:         *  .*..***:****:*.      . .

Mflv_1315|M.gilvum_PYR-GCK          VTQWVPFADWADGRVLLDDRRPTLADLDYHLTTLFPPVRPRGFLEVRYLD
Mvan_5486|M.vanbaalenii_PYR-1       VTQWVPFADWADGRVLLDDRPPTLADLDYHLTTLFPPVRPRGFLEVRYLD
MSMEG_6250|M.smegmatis_MC2_155      VTNWVPFADWADGRAVLGGRRPTEADLDYHLTTLFPPVRPRRWLEIRYLD
TH_0772|M.thermoresistible__bu      VTDWVPFADWADGRTALGGRRPTVADLEYHLTTLFPPVRPRGWLEIRYLD
Rv3704c|M.tuberculosis_H37Rv        VTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
Mb3730c|M.bovis_AF2122/97           VTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
MMAR_5215|M.marinum_M               VTHHVPFADWVDGRVLLGDRRPTYADLDYHLTTLFPPVRPRQWLEIRYLD
MAV_0399|M.avium_104                VTRWVPFADWVDGRVLLGGRRPTVADLEYHLTTLFPPVRPRQWLEIRYLD
MAB_0369|M.abscessus_ATCC_1997      VVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRWLELRYLD
                                    *.  *** .*.**   * .* .*  ** ************* :**:****

Mflv_1315|M.gilvum_PYR-GCK          SVPDAVWPAVVFTLSTLLDDPVAADVAAEATEPVATSWDRAAQLGLDDRR
Mvan_5486|M.vanbaalenii_PYR-1       SVPDATWPAVVYTLATLLDDPAAADVAAEATEEVATAWDRAAQLGLRDRR
MSMEG_6250|M.smegmatis_MC2_155      SVPDALWPAAVFTLTTLLDDPVAAESAAEATRPVATAWDRAARMGLTDRH
TH_0772|M.thermoresistible__bu      CVPDALWPAVVFTLVTLLDDPSAADIAAEATEPVSSAWSRAARDGLADRR
Rv3704c|M.tuberculosis_H37Rv        SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRR
Mb3730c|M.bovis_AF2122/97           SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRR
MMAR_5215|M.marinum_M               SLSDVFWPAVVFTLVTLLDDPGAAELAFEAVQPVATEWDTAARAGLGDPR
MAV_0399|M.avium_104                SVPDTLWPAVVFTLVALLDDPVAAGLAARAVEPVATAWDVAARVGLRDRR
MAB_0369|M.abscessus_ATCC_1997      AAPDWWWPALAFTAVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPA
                                    . .*  *** .:*  : **** .*  *   .. *.. *. **: ** *  

Mflv_1315|M.gilvum_PYR-GCK          LHTAAVRCVYAAADRAPAALVESMELLTRSVEQGRSPGDDFADRVVNDGI
Mvan_5486|M.vanbaalenii_PYR-1       LHAAATRCVQAAADRAPRALEESMGHLVRSVEQGRSPADDFADRVVAHGI
MSMEG_6250|M.smegmatis_MC2_155      LHTAALTCVRLAAERAPAELEESMTLLMRSVQQRRSPADDFSDRVVARGI
TH_0772|M.thermoresistible__bu      LFTAANRCVRTAAERTPAPLLESMERLVRSVERGRCPADDFADLVVRRGI
Rv3704c|M.tuberculosis_H37Rv        LYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
Mb3730c|M.bovis_AF2122/97           LYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
MMAR_5215|M.marinum_M               LHAAASRCVAIAAERAPAELADALQSLLAYVQRGRCPGDDFGDQVTEHGV
MAV_0399|M.avium_104                LYAAANACLAIAAERAPAELAEPMRRLVRLVEQGRCPGDDFADQVIEHGI
MAB_0369|M.abscessus_ATCC_1997      LHAAAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFIDNVTEYGV
                                    *. **   :  *.  .*  *   :  *   *::  .*.*** * *   *:

Mflv_1315|M.gilvum_PYR-GCK          AAAVTRLAQGDM---
Mvan_5486|M.vanbaalenii_PYR-1       ADAVTELAQGEL---
MSMEG_6250|M.smegmatis_MC2_155      AAAVRELAKGEL---
TH_0772|M.thermoresistible__bu      GDAVDRHGRGDW---
Rv3704c|M.tuberculosis_H37Rv        ASAVTGMMHGAS---
Mb3730c|M.bovis_AF2122/97           ASAVTGMMHGAS---
MMAR_5215|M.marinum_M               APAIARLARGEQ---
MAV_0399|M.avium_104                AASVSRAARGPQGGP
MAB_0369|M.abscessus_ATCC_1997      EKAFSGAIG------
                                      :.

species	id	gene	e-value	identity (len)	annotation
M. tuberculosis H37Rv	Rv3704c	gshA	-	100% (432)	GLUTAMATE--CYSTEINE LIGASE GSHA (GAMMA-GLUTAMYLCYSTEINE SYNTHETASE) (GAMMA-ECS) (GCS) (GAMMA-GLUTAMYL-L-CYSTEINE SYNTHETASE)